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https://www.readbyqxmd.com/read/29929120/diurnal-concentrations-sources-and-cancer-risk-assessments-of-pm-2-5-bound-pahs-npahs-and-opahs-in-urban-marine-and-mountain-environments
#1
Junmei Zhang, Lingxiao Yang, Abdelwahid Mellouki, Jianmin Chen, Xiangfeng Chen, Ying Gao, Pan Jiang, Yanyan Li, Hao Yu, Wenxing Wang
Ambient measurements of PM2.5 -bounded polycyclic aromatic hydrocarbons (PAHs), nitro-PAHs (NPAHs), and oxy-PAHs (OPAHs) were conducted during the summer in Jinan, China, an urban site, and at Tuoji island and Mt. Tai, two background locations. 3.5 h and 11.5 h sampling intervals in daytime and nighttime were utilized to research the diurnal variations of PAHs, NPAHs, and OPAHs. The concentrations of PAHs, NPAHs, and OPAHs were highest at the urban site and lowest at the marine site. The diurnal patterns of PAHs and NPAHs at the urban and marine sites were dissimilar to those observed at the mountain site partly due to the influence of the boundary layer...
June 14, 2018: Chemosphere
https://www.readbyqxmd.com/read/29928874/17%C3%AE-estradiol-protects-serum-deprivation-induced-apoptosis-and-expression-of-mmp-3-and-mmp-13-through-promoting-autophagy-in-rat-nucleus-pulposus-cells
#2
Peng Ao, Wenzhou Huang, Jian Li, Tianlong Wu, Libiao Xu, Zhongbo Deng, Wenjie Chen, Changchang Yin, Xigao Cheng
Serum deprivation is a likely contributor to intervertebral disc (IVD) degeneration (IVDD).17β-estradiol (E2) have been noted to protect nucleus pulposus cells (NPCs) against apoptosis. Autophagy and apoptosis play a paramount role in maintaining the homeostasis of IVD. So far, little research has been published on whether autophagy could take place at this very moment when E2 provides protection for NPCs. The aim of this study is to understand whether autophagy is involved in the protective effect of E2 against serum deprivation-induced cell apoptosis and expression of matrix metalloproteinase (MMP)-3 and MMP-13...
June 18, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29928497/a-superior-bright-nir-luminescent-nanoparticle-preparation-and-indicating-calcium-signaling-detection-in-cells-and-small-animals
#3
Jian Zhang, Joseph R Lakowicz
Background: Near-field fluorescence (NFF) effects were employed to develop a novel near-infrared (NIR) luminescent nanoparticle (LNP) with superior brightness. The LNP is used as imaging contrast agent for cellular and small animal imaging and furthermore suggested to use for detecting voltage-sensitive calcium in living cells and animals with high sensitivity. Results: NIR Indocyanine green (ICG) dye was conjugated with human serum albumin (HSA) followed by covalently binding to gold nanorod (AuNR)...
2018: Cell & Bioscience
https://www.readbyqxmd.com/read/29928153/evaluation-of-pre-deliric-prediction-of-delirium-in-icu-patients-delirium-prediction-model-for-the-patients-in-the-intensive-care-unit
#4
Gabrielė Linkaitė, Mantas Riauka, Ignė Bunevičiūtė, Saulius Vosylius
Introduction: Delirium not only compromises patient care, but is also associated with poorer outcomes: increased duration of mechanical ventilation, higher mortality, and greater long-term cognitive dysfunction. The PRE-DELIRIC model is a tool used to calculate the risk of the development of delirium. The classification of the patients into groups by risk allows efficient initiation of preventive measures. The goal of this study was to validate the PRE-DELIRIC model using the CAM-ICU (The Confusion Assessment Method for the Intensive Care Unit) method for the diagnosis of delirium...
2018: Acta Medica Lituanica
https://www.readbyqxmd.com/read/29927940/vector-competence-of-aedes-aegypti-culex-tarsalis-and-culex-quinquefasciatus-from-california-for-zika-virus
#5
Bradley J Main, Jay Nicholson, Olivia C Winokur, Cody Steiner, Kasen K Riemersma, Jackson Stuart, Ryan Takeshita, Michelle Krasnec, Christopher M Barker, Lark L Coffey
Zika virus (ZIKV) has emerged since 2013 as a significant global human health threat following outbreaks in the Pacific Islands and rapid spread throughout South and Central America. Severe congenital and neurological sequelae have been linked to ZIKV infections. Assessing the ability of common mosquito species to transmit ZIKV and characterizing variation in mosquito transmission of different ZIKV strains is important for estimating regional outbreak potential and for prioritizing local mosquito control strategies for Aedes and Culex species...
June 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29927833/critical-ischemia-time-perfusion-and-drainage-function-of-vascularized-lymph-nodes
#6
Chin-Yu Yang, Olivia A Ho, Ming-Huei Cheng, Hui-Yi Hsiao
BACKGROUND: Vascularized lymph node transfer is a promising surgical treatment for lymphedema. This study investigates the effect of ischemia on the lymphatic drainage efficiency of vascularized lymph node flaps and the critical ischemia time of lymph nodes. METHODS: Twenty-four lymph nodes containing groin flaps in twelve Sprague-Dawley rats were dissected. Clamping of the vascular pedicle was performed for 0, 1, 3, 5, 6, 7 hours, then each allowed to re-perfuse via the vascular pedicle for one hour...
June 12, 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29927766/role-of-nursing-informatics-in-the-automation-of-pneumonia-quality-measure-data-elements
#7
Megha Kalsy, Jau-Huei Lin, Bruce E Bray, Katherine A Sward
Core measures are standard metrics to reflect the processes of care provided by hospitals. Hospitals in the United States are expected to extract data from electronic health records, automated computation of core measures, and electronic submission of the quality measures data. Traditional manual calculation processes are time intensive and susceptible to error. Automated calculation has the potential to provide timely, accurate information, which could guide quality-of-care decisions, but this vision has yet to be achieved...
June 21, 2018: Computers, Informatics, Nursing: CIN
https://www.readbyqxmd.com/read/29927731/rats-with-higher-intrinsic-exercise-capacities-exhibit-greater-preoptic-dopamine-levels-and-greater-mechanical-and-thermoregulatory-efficiencies-while-running
#8
Patricia C R Rabelo, Letícia Maria Souza Cordeiro, Nayara Soares Sena Aquino, Bruno B B Fonseca, Cândido Celso Coimbra, Samuel Penna Wanner, Raphael Escorsim Szawka, Danusa Dias Soares
The present study investigated whether intrinsic exercise capacity affects the changes in thermoregulation, metabolism and central dopamine (DA) induced by treadmill running. Male Wistar rats were subjected to three incremental exercises and ranked as low-performance (LP), standard-performance (SP) and high-performance (HP) rats. In the first experiment, abdominal (TABD ) and tail (TTAIL ) temperatures were registered in these rats during submaximal exercise (SE) at 60% of maximal speed. Immediately after SE, rats were euthanized and concentrations of DA and 3,4-dihydroxyphenylacetic acid (DOPAC) were determined in the preoptic area (POA)...
June 21, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/29927555/assessment-of-topographic-memory-in-mice-in-a-complex-environment-using-the-hamlet-test
#9
Lucie Crouzier, Tangui Maurice
Here we provide instructions to measure topographic memory in mice using the Hamlet test, a complex environment. The apparatus mimics a small hamlet with a central agora and five houses, which are functionalized since mice can drink, eat pellets, hide within a small maze, run in an activity wheel, or interact with a stranger mouse behind a grid. The houses are interconnected through a network of streets in a five-arm star shape, and a video tracking system takes information from the activity in each house or follows a single mouse by trajectometry...
June 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29927460/are-high-flow-arteriovenous-accesses-associated-with-worse-haemodialysis
#10
Ivo Laranjinha, Patrícia Matias, Ana Azevedo, David Navarro, Carina Ferreira, Tiago Amaral, Marco Mendes, Inês Aires, Cristina Jorge, Célia Gil, Anibal Ferreira
INTRODUCTION: An arteriovenous (AV) access flow (Qa) of 400 mL/min is usually sufficient for an effective hemodialysis (HD), but some accesses continue developing and become high flow accesses (HFA). Some authors postulated that an HFA might shift a significant portion of dialyzed blood from the cardiac output, which could decrease HD efficiency and lead to volume overload. OBJECTIVE: The aim of our study was to evaluate if HFA is associated with reduced HD efficiency and/or volume overload in prevalent HD patients...
May 28, 2018: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
https://www.readbyqxmd.com/read/29927123/synthesis-characterization-and-function-of-an-rna-based-transfection-reagent
#11
Harsh V Jain, Jessica F Boehler, Kanneboyina Nagaraju, Serge L Beaucage
A synthetic 8-mer, amphipathic, trans-acting poly-2'-O-methyluridylic thiophosphate triester RNA element (2'-OMeUtaPS) can be prepared using solid-phase synthesis protocols. 2'-OMeUtaPS efficiently mediates the delivery of uncharged polyA-tailed phosphorodiamidate morpholino (PMO) sequences in HeLa pLuc 705 cells, as evidenced by flow cytometry measurements. In this cell line, 2'-OMeUtaPS-mediated transfection of an antisense polyA-tailed PMO sequence induces alternative splicing of an aberrant luciferase pre-mRNA splice site, leading to restoration of functional luciferase, as quantitatively measured using a typical luciferase assay...
March 2018: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/29926723/thick-layered-semiconductor-devices-with-water-top-gates-high-on-off-ratio-field-effect-transistors-and-aqueous-sensors
#12
Yuan Huang, Eli Sutter, Liangmei Wu, Hong Xu, Lihong Bao, Hong-Jun Gao, Xingjiang Zhou, Peter Sutter
Layered semiconductors show promise as channel materials for field-effect transistors (FETs). Usually, such devices incorporate solid back or top gate dielectrics. Here, we explore de-ionized (DI) water as a solution top gate for field-effect switching of layered semiconductors including SnS2, MoS2, and black phosphorus. The DI water gate is easily fabricated, can sustain rapid bias changes, and its efficient coupling to layered materials provides high on-off current ratios, near-ideal sub-threshold swing, and enhanced short-channel behavior even for FETs with thick, bulk-like channels where such control is difficult to realize with conventional back-gating...
June 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29926704/a-simple-enzymatic-method-for-quantitation-of-2-fucosyllactose
#13
Emine Seydametova, Jonghyeok Shin, Jiwon Yu, Dae-Hyuk Kweon
2'-Fucosyllactose (2'-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2'-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2'-FL in a microplate format. The method involves two steps: (1) release of L-fucose from 2'-FL by α-(1-2,3,4,6)-L-fucosidase and (2) measurement of NADPH formed during oxidation of L-fucose by L-fucose dehydrogenase...
May 11, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29926518/on-surface-fenton-and-fenton-like-reactions-appraised-by-paper-spray-ionization-mass-spectrometry
#14
S F Resende, B S Oliveira, R Augusti
On-surface degradation of sildenafil (an adequate substrate as it contains assorted functional groups in its structure) promoted by the Fenton (Fe2+ / H2 O2 ) and Fenton-like (Mn+ / H2 O2 ; Mn+ = Fe3+ , Co2+ , Cu2+ , Mn2+ ) systems was investigated by using paper spray ionization mass spectrometry (PS-MS). The performance of each system was compared by measuring the ratio between the relative intensities of the ions of m/z 475 (protonated sildenafil) and m/z 235 (protonated lidocaine, used as a convenient internal standard and added to the paper just before the PS-MS analyzes)...
June 21, 2018: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29926441/comparing-computer-adaptive-testing-stopping-rules-under-the-generalized-partial-credit-model
#15
Rose E Stafford, Christopher R Runyon, Jodi M Casabianca, Barbara G Dodd
An important consideration of any computer adaptive testing (CAT) program is the criterion used for ending item administration-the stopping rule, which ensures that all examinees are assessed to the same standard. Although various stopping rules exist, none of them have been compared under the generalized partial-credit model (Muraki in Applied Psychological Measurement, 16, 159-176, 1992). In this simulation study we compared the performance of three variable-length stopping rules-standard error (SE), minimum information (MI), and change in theta (CT)-both in isolation and in combination with requirements of minimum and maximum numbers of items, as well as a fixed-length stopping rule...
June 20, 2018: Behavior Research Methods
https://www.readbyqxmd.com/read/29926255/light-acclimation-of-shade-tolerant-and-sun-resistant-tradescantia-species-photochemical-activity-of-psii-and-its-sensitivity-to-heat-treatment
#16
Michael A Benkov, Anton M Yatsenko, Alexander N Tikhonov
In this work, we have compared photosynthetic characteristics of photosystem II (PSII) in Tradescantia leaves of two contrasting ecotypes grown under the low light (LL) and high light (HL) regimes during their entire growth period. Plants of the same genus, T. fluminensis (shade-tolerant) and T. sillamontana (sun-resistant), were cultivated at 50-125 µmol photons m-2  s-1 (LL) or at 875-1000 µmol photons m-2  s-1 (HL). Analyses of intrinsic PSII efficiency was based on measurements of fast chlorophyll (Chl) a fluorescence kinetics (the OJIP test)...
June 20, 2018: Photosynthesis Research
https://www.readbyqxmd.com/read/29926246/relationship-focused-vs-structural-activities-in-medical-home-measurement-in-pediatrics
#17
Christopher J Stille, Jean L Raphael, Adam C Carle, David M Keller, Renee M Turchi, Colleen A Kraft, Marie Y Mann, Dana Bright
Objectives The Family-Centered Medical Home (FCMH) has become a model of effective and efficient primary care. However, efforts to measure the FCMH may ignore its complexity. We sought to determine whether U.S. primary care pediatricians view structure-focused and relationship-focused practice activities of the FCMH as distinct constructs and how these constructs are associated with practice functions. Methods We analyzed data from the 2012 American Academy of Pediatrics Periodic Survey of Fellows #79 (n = 572) addressing opinions and practice activities related to the FCMH...
June 20, 2018: Maternal and Child Health Journal
https://www.readbyqxmd.com/read/29925872/aerosol-optical-microphysical-chemical-and-radiative-properties-of-high-aerosol-load-cases-over-the-arctic-based-on-aeronet-measurements
#18
Yisong Xie, Zhengqiang Li, Li Li, Richard Wagener, Ihab Abboud, Kaitao Li, Donghui Li, Ying Zhang, Xingfeng Chen, Hua Xu
Columnar mass concentrations of aerosol components over the Arctic are estimated using microphysical parameters derived from direct sun extinction and sky radiance measurements of Aerosol Robotic Network. Aerosol optical, microphysical, chemical and radiative properties show that Arctic aerosols are dominated by fine mode particles, especially for high aerosol load cases. The average aerosol optical depth (AOD) of the selected Arctic sites in the sampling period is approximately 0.08, with 75% composed of fine mode particles...
June 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29925746/a-new-method-for-determining-the-mycelial-weight-of-the-koji-mold-aspergillus-oryzae-by-measuring-its-glycosylceramide-content
#19
Jannatul Ferdouse, Miyuki Miyagawa, Mikako Hirano, Yuka Kitajima, Shigeki Inaba, Hiroshi Kitagaki
At present, the quantitation of the mycelial weight of the industrially important non-pathogenic fungus Aspergillus oryzae, which is used for manufacturing koji, is performed by quantitating N-acetylglucosamine. However, since N-acetylglucosamine is a cell wall component, the extraction procedure is costly and tedious, and its quantitative performance is poor. Here, we report a novel method for the quantitation of A. oryzae mycelial weight. The amount of glycosylceramide significantly correlated with both the mycelial weight of A...
June 21, 2018: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29925495/the-impact-of-implementation-of-a-clinically-integrated-problem-based-neonatal-electronic-health-record-on-documentation-metrics-provider-satisfaction-and-hospital-reimbursement-a-quality-improvement-project
#20
William Liu, Thomas Walsh
BACKGROUND: A goal of effective electronic health record provider documentation platforms is to provide an efficient, concise, and comprehensive notation system that will effectively reflect the clinical course, including the diagnoses, treatments, and interventions. OBJECTIVE: The aim is to fully redesign and standardize the provider documentation process, seeking improvement in documentation based on ongoing All Patient Refined Diagnosis Related Group-based coding records, while maintaining noninferiority comparing provider satisfaction to our existing documentation process...
June 20, 2018: JMIR Medical Informatics
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