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Lukasz P Kozlowski
BACKGROUND: Accurate estimation of the isoelectric point (pI) based on the amino acid sequence is useful for many analytical biochemistry and proteomics techniques such as 2-D polyacrylamide gel electrophoresis, or capillary isoelectric focusing used in combination with high-throughput mass spectrometry. Additionally, pI estimation can be helpful during protein crystallization trials. RESULTS: Here, I present the Isoelectric Point Calculator (IPC), a web service and a standalone program for the accurate estimation of protein and peptide pI using different sets of dissociation constant (pKa) values, including two new computationally optimized pKa sets...
October 21, 2016: Biology Direct
N S Mehl, S Srisuwatanasagul, T Swangchan-Uthai, S Sirivaidyapong, M Khalid
Effect of a GnRH-agonist (deslorelin) was studied on reproductive function and ovarian luteinizing hormone receptor (LHR) and follicle stimulating hormone receptor (FSHR) expression in prepubertal female cats that were either implanted with 4.7-mg deslorelin (implanted: n = 6) or not (controls: n = 18) or ovariohysterectomized at prepubertal age (prepubertal OVH: n = 6). Body weights, fecal estradiol, and sexual behavior of implanted and control cats were monitored for 48 weeks followed by collection of ovaries and uteri...
September 9, 2016: Theriogenology
Preeti Sharma, Vijay Wadhwan, Pooja Aggarwal, Anamika Sharma
BACKGROUND: Oral verrucous hyperplasia (OVH) and oral verrucous carcinoma (OVC) are two distinct clinicopathologic verrucous lesions. However, the distinction between the two lesions still remains enigmatic. It is almost impossible to distinguish them clinically. Thus, the final diagnosis rests on the histopathological characteristics of both lesions, being distinguished from each other by an exophytic and endophytic growth pattern, respectively. METHODS: This institutional study was planned to review retrospectively two series of patients with histologic diagnoses of VH (n = 27) and VC (n = 27) to investigate their clinicopathological features and to analyze the role of immunohistochemical (IHC) marker p53 protein in distinguishing between the two verrucous lesions...
September 2016: Journal of Oral and Maxillofacial Pathology: JOMFP
Zhengdong Zhou, Wenwen Zhang, Shaolin Guan
To effectively calculate an overlap volume histogram (OVH) descriptor and improve intensity modulated radiation treatment (IMRT) planning by basing it on previous plans with similar features, a method based on morphology for OVH calculation was proposed and a novel similarity measurement was employed for retrieval of a suitable IMRT plan. First, the minimum and maximum distances between the tumor and organs at risk (OARs) were calculated as the start and end points for contraction or expansion, and a suitable step size for contraction or expansion was determined according to these distances...
September 10, 2016: Physica Medica: PM
L Soler, N García, A Unzueta, M Piñeiro, M A Álava, F Lampreave
Inter-α-trypsin inhibitor heavy chain 4 (ITIH4) and C-reactive protein (CRP) have been isolated from acute phase dog sera by affinity chromatography with insolubilized polyclonal antibodies anti pig Major Acute phase Protein (Pig-MAP) and with p-Aminophenyl Phosphoryl Choline, respectively. Isolated proteins were used to prepare specific polyclonal rabbit antisera that have allowed quantifying their concentration in serum samples by single radial immunodifussion. Both proteins were quantified in sera from female dogs that had undergone ovariohysterectomy (OVH, n=9) or mastectomy (n=10)...
October 15, 2016: Veterinary Immunology and Immunopathology
Heyman Luckraz, Prabhjeet Kaur, Moninder Bhabra, Pankaj Kumar Mishra, Kumaresan Nagarajan, Nelam Kumari, Kamran Saleem, Alan M Nevill
BACKGROUND: A cost-benefit analysis of endoscopic vein harvesting (EVH) versus open vein harvest (OVH) was performed in patients at high risk for wound complications. METHODS: Risk factors for leg wound infection were identified as age older than 75 years, being a woman, body mass index > 28, having diabetes, being a smoker, and diagnosis of peripheral vascular disease. Patients who had at least 2 of these risk factors were selected for a pilot use of EVH and were matched to patients undergoing OVH (n = 50 patients/group)...
August 30, 2016: American Journal of Infection Control
Wei-Hua Zhang, Jie Li, Xin Zhang, Hong Luo, Ning Ma, Dong-Hai Liu, Chen-Hui Qiao, Ji-Feng Li, Yao-Bin Zhu
OBJECTIVE: To evaluate the mid-term clinical outcome of endoscopic greater saphenous vein harvesting (EVH) in coronary artery bypass grafting (CABG). Method A total of 205 patients receiving off-pump CABG between July, 2012 and April, 2013 at our department were enrolled in this study, including 66 patients (35 male and 31 female patients with a mean age of 60.3±7.92 years) undergoing EVH and 139 patients (109 male and 30 female patients with a mean age of 59.20±8.37 years) undergoing open greater saphenous vein harvesting (OVH)...
June 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Yibing Wang, Sebastiaan Breedveld, Ben Heijmen, Steven F Petit
IMRT planning with commercial Treatment Planning Systems (TPSs) is a trial-and-error process. Consequently, the quality of treatment plans may not be consistent among patients, planners and institutions. Recently, different plan quality assurance (QA) models have been proposed, that could flag and guide improvement of suboptimal treatment plans. However, the performance of these models was validated using plans that were created using the conventional trail-and-error treatment planning process. Consequently, it is challenging to assess and compare quantitatively the accuracy of different treatment planning QA models...
June 7, 2016: Physics in Medicine and Biology
Emma K Read, Andrea Vallevand, Robin M Farrell
This paper describes the development and evaluation of training intended to enhance students' performance on their first live-animal ovariohysterectomy (OVH). Cognitive task analysis informed a seven-page lab manual, 30-minute video, and 46-item OVH checklist (categorized into nine surgery components and three phases of surgery). We compared two spay simulator models (higher-fidelity silicone versus lower-fidelity cloth and foam). Third-year veterinary students were randomly assigned to a training intervention: lab manual and video only; lab manual, video, and $675 silicone-based model; lab manual, video, and $64 cloth and foam model...
2016: Journal of Veterinary Medical Education
Sikandar Z Khan, Mariel Rivero, Brian McCraith, Linda M Harris, Maciej L Dryjski, Hasan H Dosluoglu
OBJECTIVE: Although endoscopic vein harvest (EVH) has been reported to reduce the morbidity and length of stay compared with open vein harvest (OVH) for infrainguinal bypass procedures, there have been concerns about decreased graft patency and increased rates of reinterventions with EVH compared with OVH. We started using EVH in 2008, and currently it is our preferred approach. The goal of this study was to see if EVH is comparable to OVH in terms of graft patency and limb salvage and associated with fewer wound complications...
June 2016: Journal of Vascular Surgery
Ahmad Amouzeshi, Mohamad Abbassi Teshnisi, Nahid Zirak, Alireza Sepehri Shamloo, Hamid Hoseinikhah, Behzad Alizadeh, Aliasghar Moeinipour
INTRODUCTION: Harvesting of the greater saphenous vein is almost an inevitable part of coronary artery bypass grafting (CABG) operations, and it is done by two main techniques, i.e., conventional or open vein harvesting (OVH) and the minimally-invasive endoscopic vein harvesting (EVH). This study aimed to compare these two techniques in off-pump CABG procedures with respect to clinical and pathological outcomes. METHODS: This cohort study was conducted on CABG candidates during a one-year period from October 2013 through September 2014 in the Department of Cardiac Surgery at Mashhad University of Medical Sciences...
January 2016: Electronic Physician
Leon N Warne, Thierry Beths, Jennifer E Carter, Ted Whittem, Sébastien H Bauquier
OBJECTIVE: To evaluate the influence of atipamezole on postoperative pain scores in cats. STUDY DESIGN: Controlled, randomized, masked clinical trial. ANIMALS: Twelve healthy female domestic cats. METHODS: Cats admitted for ovariohysterectomy (OVH) surgery were randomly allocated to group atipamezole (n = 6) or group saline (n = 6) and were premedicated with buprenorphine 20 μg kg(-1) intramuscularly (IM) and alfaxalone 3...
July 2016: Veterinary Anaesthesia and Analgesia
Devin B Lemmex, Yohei Ono, Carol R Reno, David A Hart, Ian K Y Lo, Gail M Thornton
This study investigated whether ovariohysterectomy (OVH) surgery to induce menopause resulted in changes to modulus, failure strain and lubricin/proteoglycan 4 (PRG4) gene expression in rabbit medial collateral ligaments (MCLs), similar to aging (Thornton et al., 2015a). The MCLs from adult rabbits that underwent OVH surgery as adolescents (15-week-old) and adults (1-year-old) were compared by evaluating mechanical behaviour (adolescent OVH, n=8; adult OVH, n=7; normal, n=7), gene expression (adolescent OVH, n=9; adult OVH, n=8; normal, n=8), and collagen and glycosaminoglycan (adolescent OVH, n=9; adult OVH, n=8; normal, n=8) and water (adolescent OVH, n=9; adult OVH, n=8; normal, n=8) content...
February 8, 2016: Journal of Biomechanics
D Shin, S Kang, D Kim, T Kim, K Kim, M Cho, T Suh
PURPOSE: The dose difference between three-dimensional dose (3D dose) and 4D dose which considers motion due to respiratory can be varied according to geometrical relationship between planning target volume (PTV) and organ at risk (OAR). The purpose of the study is to investigate the dose difference between 3D and 4D dose using overlap volume histogram (OVH) which is an indicator that quantify geometrical relationship between a PTV and an OAR. METHODS: Five liver cancer patients who previously treated stereotactic body radiotherapy (SBRT) were investigated...
June 2015: Medical Physics
S Kang, S Kim, D Kim, T Kim, S Park, D Shin, M Cho, K Kim, T Suh
PURPOSE: End-of-exhale (EOE) phase is generally preferred for gating window because tumor position is more reproducible. However, other gating windows might be more appropriate for dose distribution perspective. In this pilot study, we proposed to utilize overlap volume histogram (OVH) to search optimized gating window and demonstrated its feasibility. METHODS: We acquired 4DCT of 10 phases for 3 lung patients (2 with a target at right middle lobe and 1 at right upper lobe)...
June 2015: Medical Physics
Z Cheng, J Moore, L Rosati, O Mian, A Narang, J Herman, T McNutt
PURPOSE: In radiotherapy, size, location and proximity of the target to critical structures influence treatment decisions. It has been shown that proximity of the target predicts dosimetric sparing of critical structures. In addition to dosimetry, precise location of disease has further implications such as tumor invasion, or proximity to major arteries that inhibit surgery. Knowledge of which patients can be converted to surgical candidates by radiation may have high impact on future treat/no-treat decisions...
June 2015: Medical Physics
Shankargouda Patil, Saman Warnakulasuriya, Thirumal Raj, D S Sanketh, Roopa S Rao
OBJECTIVE: Exophytic oral verrucous hyperplasia (OVH) is a new entity described by an expert working group from South Asia. First reported in Taiwan, there are no reports so far from an Indian population. The aim was to use the microscopic features described by the expert group to differentiate OVH from other oral verruco-papillary lesions in an Indian archive. MATERIALS AND METHODS: In a retrospective multicentre study, using pathology archives, 188 verruco-papillary lesions were retrieved from pathology archives...
June 15, 2015: Journal of Investigative and Clinical Dentistry
Jan J Andreasen, Henrik Vadmann, Lars Oddershede, Hans-Henrik Tilsted, Jens B Frøkjær, Svend E Jensen
OBJECTIVES: Endoscopic vein harvest has gained widespread use in coronary artery bypass surgery. However, potential negative mid- and long-term effects following endoscopic vein harvest have been described. We aimed to compare long-term clinical outcomes following endoscopic and open vein graft harvesting. DESIGN: This study was a clinical follow-up with additional computed tomographic coronary angiography among 126 first-time bypass patients originally included in a randomized study comparing early leg wound complications and cosmetic results...
2015: Scandinavian Cardiovascular Journal: SCJ
Filipa Mira, Alexandra Costa, Eva Mendes, Pedro Azevedo, L Miguel Carreira
OBJECTIVES: This pilot study aimed to investigate whether and how music and musical genres may influence the depth of anaesthesia, as measured using changes in arterial blood pressure (ABP), including systolic blood pressure (SBP), and heart rate (HR) across three different surgical time points. METHODS: This work focused on a sample of 12 female cats (Felis catus) that were subjected to an elective ovariohysterectomy (OVH), and three different surgical time points were considered (T1, coeliotomy; T2, ligature placement and transection of the ovarian pedicle; and T3, ligature placement and transection of the uterine body)...
August 2016: Journal of Feline Medicine and Surgery
Jiazhou Wang, Xiance Jin, Kuaike Zhao, Jiayuan Peng, Jiang Xie, Junchao Chen, Zhen Zhang, Matthew Studenski, Weigang Hu
PURPOSE: To investigate the feasibility of the dosimetric Pareto front (PF) prediction based on patient's anatomic and dosimetric parameters for esophageal cancer patients. METHODS: Eighty esophagus patients in the authors' institution were enrolled in this study. A total of 2928 intensity-modulated radiotherapy plans were obtained and used to generate PF for each patient. On average, each patient had 36.6 plans. The anatomic and dosimetric features were extracted from these plans...
February 2015: Medical Physics
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