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Drosophila multidendritic neuron

Seol Hee Im, Atit A Patel, Daniel N Cox, Michael J Galko
Diabetes-associated nociceptive hypersensitivity affects diabetic patients with hard-to-treat chronic pain. Because multiple tissues are affected by systemic alterations in insulin signaling, the functional locus of insulin signaling in diabetes-associated hypersensitivity remains obscure. Here, we used Drosophila nociception/nociceptive sensitization assays to investigate the role of Insulin receptor (Insulin-like receptor, InR) in nociceptive hypersensitivity. InR mutant larvae exhibited mostly normal baseline thermal nociception (absence of injury) and normal acute thermal hypersensitivity following UV-induced injury...
May 10, 2018: Disease Models & Mechanisms
Chien-Po Liao, Hsun Li, Hsiu-Hsiang Lee, Cheng-Ting Chien, Chun-Liang Pan
Self-avoidance allows sister dendrites from the same neuron to form non-redundant coverage of the sensory territory and is important for neural circuitry functions. Here, we report an unexpected, cell-autonomous role of the Wnt-secretory factor MIG-14/Wntless in mediating dendrite self-avoidance in the C. elegans multidendritic PVD neurons. Similar findings in Drosophila suggest that this novel function of Wntless is conserved. The mig-14 mutant shows defects in dendrite self-avoidance, and ectopic MIG-14 expression triggers dendrite repulsion...
April 18, 2018: Neuron
Taylor L Follansbee, Kayla J Gjelsvik, Courtney L Brann, Aidan L McParland, Colin A Longhurst, Michael J Galko, Geoffrey K Ganter
Nociceptive sensitization is a common feature in chronic pain, but its basic cellular mechanisms are only partially understood. The present study used the Drosophila melanogaster model system and a candidate gene approach to identify novel components required for modulation of an injury-induced nociceptive sensitization pathway presumably downstream of Hedgehog. This study demonstrates that RNAi silencing of a member of the Bone Morphogenetic Protein (BMP) signaling pathway, Decapentaplegic (Dpp), specifically in the Class IV multidendritic nociceptive neuron, significantly attenuated ultraviolet injury-induced sensitization...
August 30, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Atit A Patel, Daniel N Cox
To investigate cellular, molecular and behavioral mechanisms of noxious cold detection, we developed cold plate behavioral assays and quantitative means for evaluating the predominant noxious cold-evoked contraction behavior. To characterize neural activity in response to noxious cold, we implemented a GCaMP6-based calcium imaging assay enabling in vivo studies of intracellular calcium dynamics in intact Drosophila larvae. We identified Drosophila class III multidendritic (md) sensory neurons as multimodal sensors of innocuous mechanical and noxious cold stimuli and to dissect the mechanistic bases of multimodal sensory processing we developed two independent functional assays...
July 5, 2017: Bio-protocol
Yumiko Tokusumi, Tsuyoshi Tokusumi, Robert A Schulz
In vertebrates, interaction between the nervous system and immune system is important to protect a challenged host from stress inputs from external sources. In this study, we demonstrate that sensory neurons are involved in the cellular immune response elicited by wasp infestation of Drosophila larvae. Multidendritic class IV neurons sense contacts from external stimuli and induce avoidance behaviors for host defense. Our findings show that inactivation of these sensory neurons impairs the cellular response against wasp parasitization...
May 13, 2017: Biochemical and Biophysical Research Communications
Heather N Turner, Kevin Armengol, Atit A Patel, Nathaniel J Himmel, Luis Sullivan, Srividya Chandramouli Iyer, Surajit Bhattacharya, Eswar Prasad R Iyer, Christian Landry, Michael J Galko, Daniel N Cox
The basic mechanisms underlying noxious cold perception are not well understood. We developed Drosophila assays for noxious cold responses. Larvae respond to near-freezing temperatures via a mutually exclusive set of singular behaviors-in particular, a full-body contraction (CT). Class III (CIII) multidendritic sensory neurons are specifically activated by cold and optogenetic activation of these neurons elicits CT. Blocking synaptic transmission in CIII neurons inhibits CT. Genetically, the transient receptor potential (TRP) channels Trpm, NompC, and Polycystic kidney disease 2 (Pkd2) are expressed in CIII neurons, where each is required for CT...
December 5, 2016: Current Biology: CB
Siddharth Jayakumar, Shlesha Richhariya, O Venkateswara Reddy, Michael J Texada, Gaiti Hasan
Neuronal circuits are known to integrate nutritional information, but the identity of the circuit components is not completely understood. Amino acids are a class of nutrients that are vital for the growth and function of an organism. Here, we report a neuronal circuit that allows Drosophila larvae to overcome amino acid deprivation and pupariate. We find that nutrient stress is sensed by the class IV multidendritic cholinergic neurons. Through live calcium imaging experiments, we show that these cholinergic stimuli are conveyed to glutamatergic neurons in the ventral ganglion through mAChR...
August 5, 2016: ELife
Yali V Zhang, Timothy J Aikin, Zhengzheng Li, Craig Montell
Food texture has enormous effects on food preferences. However, the mechanosensory cells and key molecules responsible for sensing the physical properties of food are unknown. Here, we show that akin to mammals, the fruit fly, Drosophila melanogaster, prefers food with a specific hardness or viscosity. This food texture discrimination depends upon a previously unknown multidendritic (md-L) neuron, which extends elaborate dendritic arbors innervating the bases of taste hairs. The md-L neurons exhibit directional selectivity in response to mechanical stimuli...
August 17, 2016: Neuron
Simona Antonacci, Daniel Forand, Margaret Wolf, Courtney Tyus, Julia Barney, Leah Kellogg, Margo A Simon, Genevieve Kerr, Kristen L Wells, Serena Younes, Nathan T Mortimer, Eugenia C Olesnicky, Darrell J Killian
The regulation of dendritic branching is critical for sensory reception, cell-cell communication within the nervous system, learning, memory, and behavior. Defects in dendrite morphology are associated with several neurologic disorders; thus, an understanding of the molecular mechanisms that govern dendrite morphogenesis is important. Recent investigations of dendrite morphogenesis have highlighted the importance of gene regulation at the posttranscriptional level. Because RNA-binding proteins mediate many posttranscriptional mechanisms, we decided to investigate the extent to which conserved RNA-binding proteins contribute to dendrite morphogenesis across phyla...
April 2015: G3: Genes—Genomes—Genetics
Stephanie E Mauthner, Richard Y Hwang, Amanda H Lewis, Qi Xiao, Asako Tsubouchi, Yu Wang, Ken Honjo, J H Pate Skene, Jörg Grandl, W Daniel Tracey
The Drosophila gene pickpocket (ppk) encodes an ion channel subunit of the degenerin/epithelial sodium channel (DEG/ENaC) family. PPK is specifically expressed in nociceptive, class IV multidendritic (md) neurons and is functionally required for mechanical nociception responses. In this study, in a genome-wide genetic screen for other ion channel subunits required for mechanical nociception, we identify a gene that we name balboa (also known as CG8546, ppk26). Interestingly, the balboa locus encodes a DEG/ENaC ion channel subunit highly similar in amino acid sequence to PPK...
December 15, 2014: Current Biology: CB
Josh S Titlow, Jordan Rice, Zana R Majeed, Emily Holsopple, Stephanie Biecker, Robin L Cooper
Afferent neurons commonly exhibit a somatotopic arrangement in the central nervous system that organizes spatially discrete sensory input. We are interested in how that spatial input gets integrated into motor commands. With resources for screening genes and neural circuits, and given that the cells and ion channels that transduce tactile stimuli in Drosophila larvae are remarkably well-characterized, larval mechanosensation is an ideal system for investigating how specific behaviors emerge from localized sensory input...
June 2014: Neuroscience Research
Tomoko Ohyama, Tihana Jovanic, Gennady Denisov, Tam C Dang, Dominik Hoffmann, Rex A Kerr, Marta Zlatic
All organisms react to noxious and mechanical stimuli but we still lack a complete understanding of cellular and molecular mechanisms by which somatosensory information is transformed into appropriate motor outputs. The small number of neurons and excellent genetic tools make Drosophila larva an especially tractable model system in which to address this problem. We developed high throughput assays with which we can simultaneously expose more than 1,000 larvae per man-hour to precisely timed noxious heat, vibration, air current, or optogenetic stimuli...
2013: PloS One
Myung Jun Kim, Joshua A Ainsley, Justin W Carder, Wayne A Johnson
Reactive oxygen species (ROS) in excess have been implicated in numerous chronic illnesses, including asthma, diabetes, aging, cardiovascular disease, and neurodegenerative illness. However, at lower concentrations, ROS can also serve essential routine functions as part of cellular signal transduction pathways. As products of atmospheric oxygen, ROS-mediated signals can function to coordinate external environmental conditions with growth and development. A central challenge has been a mechanistic distinction between the toxic effects of oxidative stress and endogenous ROS functions occurring at much lower concentrations...
December 2013: Journal of Neurogenetics
Satoko Hakeda, Takashi Suzuki
Precise refinement of axonal and dendritic patterns is essential for the maturation of functional neuronal circuits. Although several transmembrane molecules have been shown to control the development of both axons and dendrites, the molecular mechanisms that regulate these different processes are poorly understood. Golden Goal (Gogo) is one of the molecules that are known to control the development of axons in the Drosophila visual system. In this study, we analyzed Gogo function in dendritic field formation of dorsal multidendritic arborization (md-da) neurons of the Drosophila Peripheral Nervous System...
November 2013: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Akira Fushiki, Hiroshi Kohsaka, Akinao Nose
Neuronal circuits are formed according to a genetically predetermined program and then reconstructed in an experience-dependent manner. While the existence of experience-dependent plasticity has been demonstrated for the visual and other sensory systems, it remains unknown whether this is also the case for motor systems. Here we examined the effects of eliminating sensory inputs on the development of peristaltic movements in Drosophila embryos and larvae. The peristalsis is initially slow and uncoordinated, but gradually develops into a mature pattern during late embryonic stages...
2013: PloS One
Volodymyr Kucher, Benjamin A Eaton, James D Stockand, Nina Boiko
Electrophysiological studies provide essential clues about the regulation and physiological function of ion channel proteins. Probing ion channel activity in vivo, though, often is challenging. This can limit the usefulness of such model organisms as Drosophila for electrophysiological studies. This is unfortunate because these genetically tractable organisms represent powerful research tools that facilitate elaboration of complex questions of physiology. Here, we describe a recently developed method for recording ion channel activity in Drosophila sensory neurons...
2013: Methods in Molecular Biology
Kathleen M Zelle, Beika Lu, Sarah C Pyfrom, Yehuda Ben-Shahar
Degenerin/epithelial sodium channels (DEG/ENaC) represent a large family of animal-specific membrane proteins. Although the physiological functions of most family members are not known, some have been shown to act as nonvoltage gated, amiloride-sensitive sodium channels. The DEG/ENaC family is exceptionally large in genomes of Drosophila species relative to vertebrates and other insects. To elucidate the evolutionary history of the DEG/ENaC family in Drosophila, we took advantage of the genomic and genetic information available for 12 Drosophila species that represent all the major species groups in the Drosophila clade...
March 2013: G3: Genes—Genomes—Genetics
Daisuke Satoh, Ritsuko Suyama, Ken-ichi Kimura, Tadashi Uemura
Neuronal circuits that are formed in early development are reorganized at later developmental stages to support a wide range of adult behaviors. At Drosophila pupal stages, one example of this reorganization is dendritic remodeling of multidendritic neurons, which is accomplished by pruning and subsequent regeneration of branches in environments quite distinct from those in larval life. Here, we used long-term in vivo time-lapse recordings at high spatiotemporal resolution and analyzed the dynamics of two adjacent cell types that remodel dendritic arbors, which eventually innervate the lateral plate of the adult abdomen...
December 2012: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Asako Tsubouchi, Jason C Caldwell, W Daniel Tracey
BACKGROUND: Among the Aristotelian senses, the subcellular and molecular mechanisms involved in the sense of touch are the most poorly understood. RESULTS: We demonstrate that specialized sensory neurons, the class II and class III multidendritic (md) neurons, are gentle touch sensors of Drosophila larvae. Genetic silencing of these cells significantly impairs gentle touch responses, optogenetic activation of these cells triggers behavioral touch-like responses, and optical recordings from these neurons show that they respond to force...
November 20, 2012: Current Biology: CB
Nina Boiko, Volodymyr Kucher, James D Stockand, Benjamin A Eaton
The molecular transformation of an external stimulus into changes in sensory neuron activity is incompletely described. Although a number of molecules have been identified that can respond to stimuli, evidence that these molecules can transduce stimulation into useful neural activity is lacking. Here we demonstrate that pickpocket1 (ppk1), a Drosophila homolog of mammalian Degenerin/epithelial sodium channels, encodes an acid-sensing sodium channel that conducts a transient depolarizing current in multidendritic sensory neurons of Drosophila melanogaster...
November 16, 2012: Journal of Biological Chemistry
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