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Nicotinamide adenine dinucleotide

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https://www.readbyqxmd.com/read/28820483/a-microsomal-proteomics-view-of-h%C3%A2-o%C3%A2-and-aba-dependent-responses
#1
May Alqurashi, Ludivine Thomas, Chris Gehring, Claudius Marondedze
The plant hormone abscisic acid (ABA) modulates a number of plant developmental processes and responses to stress. In planta, ABA has been shown to induce reactive oxygen species (ROS) production through the action of plasma membrane-associated nicotinamide adenine dinucleotide phosphate (NADPH)-oxidases. Although quantitative proteomics studies have been performed to identify ABA- or hydrogen peroxide (H₂O₂)-dependent proteins, little is known about the ABA- and H₂O₂-dependent microsomal proteome changes...
August 18, 2017: Proteomes
https://www.readbyqxmd.com/read/28816059/atherothrombosis-and-oxidative-stress-mechanisms-and-management-in-elderly
#2
Francesco Violi, Lorenzo Loffredo, Roberto Carnevale, Pasquale Pignatelli, Daniele Pastori
SIGNIFICANCE The incidence of cardiovascular events increases by aging, representing the main cause of death in old population. Aging is associated with over-production of reactive oxygen species (ROS), which may affect clotting and platelet activation, and impair endothelial function so predisposing elderly patients to thrombotic complications. RECENT ADVANCES There are increasing evidences to suggest that aging is associated with an imbalance between oxidative stress and antioxidant status. Thus, up-regulation of ROS-producing enzymes such as nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and myeloperoxidase, along with down-regulation of antioxidant enzymes, such as superoxide dismutase and glutathione peroxidase, occur during aging...
August 17, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28815642/nox4-and-nrf2-mediated-oxidative-stress-induced-by-silver-nanoparticles-in-vascular-endothelial-cells
#3
Xia Sun, Yi Yang, Junpeng Shi, Chengcheng Wang, Zhenfeng Yu, Hongwu Zhang
It has been widely reported that silver nanoparticles (AgNPs) induce oxidative stress in various cell lines. However, the mechanism for this effect and its consequences for cellular signaling are poorly understood. In this study, human umbilical vein endothelial cells (HUVECs) were used to assess the toxicity and investigate the associated molecular mechanisms caused by exposure to AgNPs. We demonstrated that AgNP exposure significantly and dose-dependently decreased the cell viability, induced reactive oxygen species (ROS) generation and led to early apoptosis in HUVECs...
August 16, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28813682/circadian-and-feeding-rhythms-orchestrate-the-diurnal-liver-acetylome
#4
Daniel Mauvoisin, Florian Atger, Loïc Dayon, Antonio Núñez Galindo, Jingkui Wang, Eva Martin, Laetitia Da Silva, Ivan Montoliu, Sebastiano Collino, Francois-Pierre Martin, Joanna Ratajczak, Carles Cantó, Martin Kussmann, Felix Naef, Frédéric Gachon
Lysine acetylation is involved in various biological processes and is considered a key reversible post-translational modification in the regulation of gene expression, enzyme activity, and subcellular localization. This post-translational modification is therefore highly relevant in the context of circadian biology, but its characterization on the proteome-wide scale and its circadian clock dependence are still poorly described. Here, we provide a comprehensive and rhythmic acetylome map of the mouse liver...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28812382/can-elevated-lactate-and-ldh-produce-a-false-positive-enzymatic-ethanol-result-in-live-patients-presenting-to-the-emergency-department
#5
Nicholas Nacca, Michael J Hodgman, Kirselle Lao, Matthew Elkins, Michael G Holland
BACKGROUND: There have been allegations in the courtroom that elevated serum lactic acid in trauma victims can yield a falsely elevated serum ethanol assay. Most hospitals utilize an indirect method of ethanol measurement where a serum sample is added to a mix of alcohol dehydrogenase and oxidized nicotinamide adenine dinucleotide (NAD+). This allows any ethanol in the patient's serum to be metabolized to acetaldehyde, and in the process results in the reduction of NAD + to NADH. NADH is then measured using spectrophotometry...
August 16, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28812311/nad-replenishment-with-nicotinamide-mononucleotide-protects-blood-brain-barrier-integrity-and-attenuates-delayed-tpa-induced-haemorrhagic-transformation-after-cerebral-ischemia
#6
Chun-Chun Wei, Yuan-Yuan Kong, Xia-Hua, Guo-Qiang Li, Si-Li Zheng, Ming-He Cheng, Pei Wang, Chao-Yu Miao
BACKGROUND AND PURPOSE: Tissue plasminogen activator (tPA) is the only approved pharmacological therapy for acute brain ischemia; however, a major limitation of tPA is the haemorrhagic transformation followed by tPA treatment. Here, we determine whether nicotinamide mononucleotide (NMN), a key intermediate of nicotinamide adenine dinucleotide biosynthesis, would affect tPA-induced haemorrhagic transformation. EXPERIMENTAL APPROACH: Middle cerebral artery occlusion (MCAO) was achieved in CD1 mice by introducing a filament to the left MCA for 5 hours...
August 15, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28810820/quantitative-label-free-evaluation-of-tissue-engineered-skeletal-muscle-through-multiphoton-microscopy
#7
Brian C Syverud, Mary-Ann Mycek, Lisa Marie Larkin
The lack of tools for assessing engineered tissue viability and function in a non-invasive manner is a major regulatory and translational challenge facing tissue engineers. Label-free, nonlinear optical molecular imaging (OMI) has utilized endogenous nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) fluorescence to indicate metabolic activity. Similarly, second harmonic generation (SHG) signals from myosin and collagen can measure overall muscle structural integrity and function...
August 16, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28808714/a-complicated-biocomputing-system-based-on-multi-responsive-p-nipam-co-apba-copolymer-film-electrodes-and-electrocatalysis-of-nadh
#8
Jiying Liang, Xue Yu, Tiangang Yang, Menglu Li, Li Shen, Yue Jin, Hongyun Liu
In this paper, poly(N-isopropylacrylamide-co-3-aminophenylboronic acid) (P(NIPAM-co-APBA)) copolymer films were successfully electropolymerized on the Au electrode surface. The electroactive probe ferrocene carboxylic acid (FCA) in solution showed reversible thermal-, glucose- and pH-responsive on-off cyclic voltammetric (CV) behaviors at the film electrodes. The comparative experiments demonstrated that the thermo-responsive property of the film electrode was ascribed to the PNIPAM component of the films, whereas the glucose- and pH-sensitive behaviors came from the PAPBA constituent...
August 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28808418/emerging-roles-of-sirt1-in-fatty-liver-diseases
#9
REVIEW
Ren-Bo Ding, Jiaolin Bao, Chu-Xia Deng
Fatty liver diseases, which are commonly associated with high-fat/calorie diet, heavy alcohol consumption and/or other metabolic disorder causes, lead to serious medical concerns worldwide in recent years. It has been demonstrated that metabolic homeostasis disruption is most likely to be responsible for this global epidemic. Sirtuins are a group of conserved nicotinamide adenine dinucleotide (NAD(+)) dependent histone and/or protein deacetylases belonging to the silent information regulator 2 (Sir2) family...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28807785/blocking-the-cd38-cadpr-pathway-plays-a-double-edged-role-in-lps-stimulated-microglia
#10
Yi-Min Wang, Zhi-Yong Liu, Yu-Hang Ai, Li-Na Zhang, Yu Zou, Qian-Yi Peng
Whether the CD38/cyclic ADP-ribose (cADPR) pathway plays a protective or detrimental role in neuroinflammation remains controversial. This study aimed to determine the role of CD38 in neuroinflammation using lipopolysaccharide (LPS)-stimulated BV2 microglial cells and co-cultured Neuro-2a (N2a) cells. In monoculture experiments, BV2 cells were divided into control, CD38 interference (CD38Ri), negative control (NC), LPS, CD38Ri+LPS, NC+LPS and 8-Br-cADPR+LPS groups. In co-culture experiments, N2a cells were co-cultured with BV2 cells for 48h...
August 12, 2017: Neuroscience
https://www.readbyqxmd.com/read/28792876/nad-deficiency-congenital-malformations-and-niacin-supplementation
#11
Hongjun Shi, Annabelle Enriquez, Melissa Rapadas, Ella M M A Martin, Roni Wang, Julie Moreau, Chai K Lim, Justin O Szot, Eddie Ip, James N Hughes, Kotaro Sugimoto, David T Humphreys, Aideen M McInerney-Leo, Paul J Leo, Ghassan J Maghzal, Jake Halliday, Janine Smith, Alison Colley, Paul R Mark, Felicity Collins, David O Sillence, David S Winlaw, Joshua W K Ho, Gilles J Guillemin, Matthew A Brown, Kazu Kikuchi, Paul Q Thomas, Roland Stocker, Eleni Giannoulatou, Gavin Chapman, Emma L Duncan, Duncan B Sparrow, Sally L Dunwoodie
BACKGROUND: Congenital malformations can be manifested as combinations of phenotypes that co-occur more often than expected by chance. In many such cases, it has proved difficult to identify a genetic cause. We sought the genetic cause of cardiac, vertebral, and renal defects, among others, in unrelated patients. METHODS: We used genomic sequencing to identify potentially pathogenic gene variants in families in which a person had multiple congenital malformations...
August 10, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/28783606/photosensitized-oxidation-of-nicotinamide-adenine-dinucleotide-by-diethoxyphosphorus-v-tetraphenylporphyrin-and-its-fluorinated-derivative-possibility-of-chain-reaction
#12
Kazutaka Hirakawa, Atsushi Murata
Water-soluble porphyrins, diethoxyphosphorus(V)tetraphenylporphyrin (EtP(V)TPP) and its fluorinated analogue (FEtP(V)TPP), decreased the typical absorption around 340nm of nicotinamide adenine dinucleotide (NADH) under visible light irradiation, indicating oxidative decomposition. A singlet oxygen quencher, sodium azide, and a triplet quencher, potassium iodide, slightly inhibited photosensitized NADH oxidation. However, these inhibitory effects were very small. Furthermore, the fluorescence lifetime of these P(V)porphyrins was decreased by NADH, suggesting the contribution of electron transfer to the singlet excited (S1) state of P(V)porphyrin...
July 31, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28772201/effective-immobilization-of-alcohol-dehydrogenase-on-carbon-nanoscaffolds-for-ethanol-biofuel-cell
#13
Yogeswaran Umasankar, Bal-Ram Adhikari, Aicheng Chen
An efficient approach for immobilizing alcohol dehydrogenase (ADH) while enhancing its electron transfer ability has been developed using poly(2-(trimethylamino)ethyl methacrylate) (MADQUAT) cationic polymer and carbon nanoscaffolds. The carbon nanoscaffolds were comprised of single-walled carbon nanotubes (SWCNTs) wrapped with reduced graphene oxide (rGO). The ADH entrapped within the MADQUAT that was present on the carbon nanoscaffolds exhibited a high electron exchange capability with the electrode through its cofactor β-nicotinamide adenine dinucleotide hydrate and β-nicotinamide adenine dinucleotide reduced disodium salt hydrate (NAD(+)/NADH) redox reaction...
July 26, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28770679/the-stomatogastric-and-enteric-nervous-system-of-the-pulmonate-snail-megalobulimus-abbreviatus-a-neurochemical-analysis
#14
Malcon Andrei Martinez-Pereira, Raphaela da Cunha Franceschi, Bárbara Paranhos Coelho, Denise M Zancan
Chemical coding of stomatogastric nervous system (STNS) and enteric nervous system (ENS) of midgut and hindgut in the snail Megalobulimus abbreviatus was investigated using histochemistry, histofluorescence, and immunohistochemistry. The gastrointestinal plexuses, constituted by intrinsic neurons and fibers originating from the subesophageal ganglia and/or STNS, showed intense acetylcholinesterase (AChE) and nicotinamide adenine dinucleotide diaphorase (NADPHd) activity. The enteric neurons and fibers with AChE activity are scattered in the submucosa and between both muscular layers of gastrointestinal tract, whereas NADPHd neurons and fibers are more abundant between muscular layers than in the submucosa...
August 2017: Zoological Science
https://www.readbyqxmd.com/read/28766915/metabolomics-analysis-reveals-distinct-profiles-of-nonmuscle-invasive-and-muscle-invasive-bladder-cancer
#15
Divya Sahu, Yair Lotan, Bryan Wittmann, Bruce Neri, Donna E Hansel
Urothelial carcinoma is the most common form of bladder cancer, but pathway changes that occur with stage-wise progression have not been well defined. We used a metabolomics approach to identify potential metabolic pathways uniquely altered in normal urothelium, nonmuscle-invasive bladder cancer (NMIBC), and muscle-invasive bladder cancer (MIBC). We performed global metabolomic profiling using GC-mass spectrometry (MS) and LC-MS platforms to identify metabolite signatures between normal urothelium and high-grade urothelial carcinoma of different stages...
August 1, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28763287/role-of-nadph-oxidase-as-a-mediator-of-oxidative-damage-in-low-dose-irradiated-and-hindlimb-unloaded-mice
#16
Xiao Wen Mao, Nina C Nishiyama, Mary Campbell-Beachler, Peter Gifford, Kristine E Haynes, Daila S Gridley, Michael J Pecaut
The purpose of this study was to determine whether nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-derived stress can account for unloading- and radiation-induced endothelial damage and neurovascular remodeling in a mouse model. Wild-type (WT, Nox2(+/+)) C57BL/6 mice or Nox2(-/-) (B6.129S6-CYBBM) knockout (KO) mice were placed into one of the following groups: age-matched control; hindlimb unloading (HLU); low-dose/low-dose-rate radiation (LDR); or HLU with LDR simultaneously for 21 days. The mice were then sacrificed one month later...
August 1, 2017: Radiation Research
https://www.readbyqxmd.com/read/28761050/non-labeling-multiphoton-excitation-microscopy-as-a-novel-diagnostic-tool-for-discriminating-normal-tissue-and-colorectal-cancer-lesions
#17
Takahiro Matsui, Hiroki Mizuno, Takao Sudo, Junichi Kikuta, Naotsugu Haraguchi, Jun-Ichiro Ikeda, Tsunekazu Mizushima, Hirofumi Yamamoto, Eiichi Morii, Masaki Mori, Masaru Ishii
Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28760603/simultaneous-determination-of-intracellular-nucleotides-and-coenzymes-in-yarrowia-lipolytica-producing-lipid-and-lycopene-by-capillary-zone-electrophoresis
#18
Chen Zhao, Yan Yang, Linfang Wei, Qinghua Guo, Hao Fang, Shaolin Chen, Qiang Hua
Yarrowia lipolytica is an oleaginous yeast with promise in producing terpenoids such as lycopene. Though methods for analyzing primary metabolic intermediates have been established, further work is needed to better analyze nucleotides and coenzymes. Here, we presented an optimized method for the separation of nucleotides and coenzymes in Y. lipolytica using the capillary electrophoresis. The separation of twelve metabolites including four coenzymes, five nucleotides and three nucleosides was achieved within 32min using a voltage of 15kV and 70mM sodium carbonate/hydrogencarbonate buffer with 1...
July 24, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28759281/balanced-caloric-restriction-minimizes-changes-caused-by-aging-on-the-colonic-myenteric-plexus
#19
Renata de Britto Mari, Sandra Regina Stabille, Haroldo Garcia de Faria, Joice Naiara Bertaglia Pereira, Juliana Plácido Guimarães, Gabriela Pustiglione Marinsek, Romeu Rodrigues de Souza
Aging can promote significant morphofunctional changes in the gastrointestinal tract (GIT). Regulation of GIT motility is mainly controlled by the myenteric neurons of the enteric nervous system. Actions that aim at decreasing the aging effects in the GIT include those related to diet, with caloric restriction (CR). The CR is achieved by controlling the amount of food or by manipulating the components of the diet. Therefore, the objective of this study was to evaluate different levels of CR on the plasticity of nicotinamide adenine dinucleotide phosphate- (NADPH-) reactive myenteric neurons in the colon of Wistar rats during the aging process using ultrastructural (transmission electron microscopy) and morphoquantitative analysis...
July 31, 2017: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/28758645/fluorometric-gas-imaging-system-sniff-cam-using-the-extinction-of-nadh-with-an-adh-reverse-reaction-for-acetaldehyde-in-the-gas-phase
#20
Kenta Iitani, Toshiyuki Sato, Munire Naisierding, Yuuki Hayakawa, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi
A gas-imaging system (sniff-cam) that allows fluorometric visualization of a two-dimensional (2-D) distribution of gaseous acetaldehyde (AcH) was developed. It employed a reverse reaction of a nicotinamide adenine dinucleotide (NADH) dependent enzyme that led to consumption of NADH in that reaction. The system was constructed with a highly sensitive camera, an ultraviolet light emitting diode array sheet, two band pass filters and an alcohol dehydrogenase (ADH)-immobilized mesh that was used for AcH detection...
July 31, 2017: Analyst
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