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https://www.readbyqxmd.com/read/27909576/zika-antiviral-chemotherapy-identification-of-drugs-and-promising-starting-points-for-drug-discovery-from-an-fda-approved-library
#1
Bruno S Pascoalino, Gilles Courtemanche, Marli T Cordeiro, Laura H V G Gil, Lucio Freitas-Junior
Background The recent epidemics of Zika virus (ZIKV) implicated it as the cause of serious and potentially lethal congenital conditions such microcephaly and other central nervous system defects, as well as the development of the Guillain-Barré syndrome in otherwise healthy patients. Recent findings showed that anti-Dengue antibodies are capable of amplifying ZIKV infection by a mechanism similar to antibody-dependent enhancement, increasing the severity of the disease. This scenario becomes potentially catastrophic when the global burden of Dengue and the advent of the newly approved anti-Dengue vaccines in the near future are taken into account...
2016: F1000Research
https://www.readbyqxmd.com/read/27906190/robust-high-throughput-kinetic-analysis-of-apoptosis-with-real-time-high-content-live-cell-imaging
#2
Jesse D Gelles, Jerry Edward Chipuk
Quantitative and kinetic analyses of apoptotic cell death are integral components of exploring cell biology, measuring cellular stress responses, and performing high-throughput genomic/RNAi/drug screens. Here, we present a detailed method that integrates robust kinetic real-time high-content imaging with Annexin V labelling to provide a highly sensitive, accurate, simple and zero-handling approach to quantify extrinsic and intrinsic inducers of apoptosis. The sensitivity of this non-toxic method outperforms previous high-throughput methodologies using viability dyes or caspase-activation reporters...
December 1, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27905887/a-new-measure-for-infant-mental-health-screening-development-and-initial-validation
#3
Janni Ammitzbøll, Bjørn E Holstein, Lisbeth Wilms, Anette Andersen, Anne Mette Skovgaard
BACKGROUND: Mental health problems are a major public health challenges, and strategies of early prevention are needed. Effective prevention depends on feasible and validated measures of screening and intervention. Previous research has demonstrated potentials for infant mental health screening by community health nurses (CHN) in existing service settings in Denmark. This study was conducted to describe the development of a service setting based measure to screen for infant mental health problems, to investigate problems identified by the measure and assess the validity and feasibility in existing public health settings...
December 1, 2016: BMC Pediatrics
https://www.readbyqxmd.com/read/27905431/the-snd-proteins-constitute-an-alternative-targeting-route-to-the-endoplasmic-reticulum
#4
Naama Aviram, Tslil Ast, Elizabeth A Costa, Eric C Arakel, Silvia G Chuartzman, Calvin H Jan, Sarah Haßdenteufel, Johanna Dudek, Martin Jung, Stefan Schorr, Richard Zimmermann, Blanche Schwappach, Jonathan S Weissman, Maya Schuldiner
In eukaryotes, up to one-third of cellular proteins are targeted to the endoplasmic reticulum, where they undergo folding, processing, sorting and trafficking to subsequent endomembrane compartments. Targeting to the endoplasmic reticulum has been shown to occur co-translationally by the signal recognition particle (SRP) pathway or post-translationally by the mammalian transmembrane recognition complex of 40 kDa (TRC40) and homologous yeast guided entry of tail-anchored proteins (GET) pathways. Despite the range of proteins that can be catered for by these two pathways, many proteins are still known to be independent of both SRP and GET, so there seems to be a critical need for an additional dedicated pathway for endoplasmic reticulum relay...
November 30, 2016: Nature
https://www.readbyqxmd.com/read/27900744/-developing-and-verifying-the-validity-and-reliability-of-the-electronic-menopausal-health-screen-system
#5
Pei-Shan Lee, Chyi-Long Lee, Chieh-Yu Liu, Wen-Chin Chen, Yen-Yen Yu, Lee-Ing Tsao
BACKGROUND: Menopausal women are in a transitional phase between health and sickness. Although the highest standards of menopausal care include clinical assessment and patient education on menopausal symptoms, current practices lack integrated care that aim to prevent chronic diseases for which menopause is a predisposing factor. PURPOSE: To integrate menopausal disturbances; to evaluate the risk factors for osteoporosis, cardiovascular disease, and diabetes; and to create a reliable and effective electronic menopausal health screen system (EMHSS)...
December 2016: Hu Li za Zhi the Journal of Nursing
https://www.readbyqxmd.com/read/27900687/cloning-and-expression-of-metagenomic-dna-in-streptomyces-lividans-and-subsequent-fermentation-for-optimized-production
#6
Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, Jozef Anné
The choice of an expression system for the metagenomic DNA of interest is of vital importance for the detection of any particular gene or gene cluster. Most of the screens to date have used the gram-negative bacterium Escherichia coli as a host for metagenomic gene libraries. However, the use of E. coli introduces a potential host bias since only 40 % of the enzymatic activities may be readily recovered by random cloning in E. coli. To recover some of the remaining 60 %, alternative cloning hosts such as Streptomyces spp...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27899692/jenkins-ci-an-open-source-continuous-integration-system-as-a-scientific-data-and-image-processing-platform
#7
Ioannis K Moutsatsos, Imtiaz Hossain, Claudia Agarinis, Fred Harbinski, Yann Abraham, Luc Dobler, Xian Zhang, Christopher J Wilson, Jeremy L Jenkins, Nicholas Holway, John Tallarico, Christian N Parker
High-throughput screening generates large volumes of heterogeneous data that require a diverse set of computational tools for management, processing, and analysis. Building integrated, scalable, and robust computational workflows for such applications is challenging but highly valuable. Scientific data integration and pipelining facilitate standardized data processing, collaboration, and reuse of best practices. We describe how Jenkins-CI, an "off-the-shelf," open-source, continuous integration system, is used to build pipelines for processing images and associated data from high-content screening (HCS)...
November 29, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27899116/the-correlation-between-antimutagenic-activity-and-total-phenolic-content-of-extracts-of-31-plant-species-with-high-antioxidant-activity
#8
Tshepiso Jan Makhafola, Esameldin Elzein Elgorashi, Lyndy Joy McGaw, Luc Verschaeve, Jacobus Nicolaas Eloff
BACKGROUND: Antimutagenic activity of plant extracts is important in the discovery of new, effective cancer preventing agents. There is increasing evidence that cancer and other mutation-related diseases can be prevented by intake of DNA protective agents. The identification of antimutagenic agents present in plants presents an effective strategy to inhibit pathogenic processes resulting from exposure to mutagenic and/or carcinogenic substances present in the environment. There are no reports on the antimutagenic activities of the plant species investigated in this study...
November 29, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27897091/inducing-mutations-through-%C3%AE-irradiation-in-seeds-of-mucuna-pruriens-for-developing-high-l-dopa-yielding-genotypes
#9
Susheel Kumar Singh, Deepti Yadav, Raj Kishori Lal, Madan M Gupta, Sunita Singh Dhawan
PURPOSE: To develop elite genotypes in Mucuna pruriens (L.) DC with high L-DOPA (L-3, 4 dihydroxyphenylalanine) yields, with non-itching characteristics and better adaptability by applying γ-irradiation. Molecular and chemical analysis was performed for screening based on specific characteristics desired for developing suitable genotypes. MATERIALS AND METHODS: Developed, mutant populations were analyzed for L-DOPA % in seeds through TLC (thin layer chromatography), and the results obtained were validated with the HPLC (High performance liquid chromatography)...
November 29, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27896580/highly-improved-acarbose-production-of-actinomyces-through-the-combination-of-artp-and-penicillin-susceptible-mutant-screening
#10
Fei Ren, Long Chen, Qunyi Tong
Atmospheric and room temperature plasma (ARTP) was first employed to generate mutants of Actinomyces JN537 for improving acarbose production. To obtain higher acarbose producing strains, the method of screening the strains for susceptibility to penicillin was used after treatment with ARTP. The rationale for the strategy was that mutants showing penicillin susceptibility were likely to be high acarbose producers, as their ability to synthesize cell walls was weak which might enhance metabolic flux to the pathway of acarbose biosynthesis...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27892811/simultaneous-determination-of-glycyrrhizin-and-liquiritin-in-licorice-roots-and-kampo-medicines-by-combination-enzyme-linked-immunosorbent-assay-using-anti-glycyrrhizin-and-anti-liquiritin-monoclonal-antibodies
#11
Shunsuke Fujii, Osamu Morinaga, Takuhiro Uto, Shuichi Nomura, Yukihiro Shoyama
Immunoassay systems using monoclonal antibodies (mAbs) are one of the most useful techniques in the analytical, biochemical, and clinical fields. In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines...
November 28, 2016: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/27890701/evaluation-for-substitution-of-stem-bark-with-small-branches-of-myrica-esculenta-for-medicinal-use-a-comparative-phytochemical-study
#12
Bhavana Srivastava, Vikas C Sharma, Pramila Pant, N K Pandey, A D Jadhav
BACKGROUND: Over exploitation of many traditional medicinal plants like Myrica esculenta has become a threat and in the near future, many medicinal plants may be unavailable for use of industry. OBJECTIVE: Present study outlines the concept of plant part substitution. Stem bark and small branches of M. esculenta are compared on the basis of physicochemical analysis, phytochemical analysis, total phenolic contents, total flavonoid contents and high performance thin layer chromatography (HPTLC) to evaluate the possibilities of using small branches in place of stem bark...
November 25, 2016: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/27889618/cell-death-mechanistic-study-of-photodynamic-therapy-against-breast-cancer-cells-utilizing-liposomal-delivery-of-5-10-15-20-tetrakis-benzo-b-thiophene-porphyrin
#13
Geewoo Nam, Sabarinathan Rangasamy, Hee Ju, Annie Agnes Suganya Samson, Joon Myong Song
5,10,15,20-Tetrakis(benzo[b]thiophene) porphyrin (BTP) is a newly synthesized hydrophobic photosensitizer with fluorescence quantum yield in toluene: ΦF=0.062. Previously, its limitations in solubility had hindered scientific experimentation regarding its photodynamic effects on cancer cells. By utilizing various compositions of liposomes in order to alter the solubility of BTP, the photocytotoxicity, reactive oxygen species generation, and subcellular localization of the liposomal BTP were identified in this work...
November 12, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27889160/evaluation-of-baker-s-yeast-in-honey-using-a-real-time-pcr-assay
#14
Christina Kast, Alexandra Roetschi
Occasionally, melissopalynological analysis reveals the presence of baker's yeast (Saccharomyces cerevisiae) in honey sediments. A field experiment reproducing a common spring bee feeding practice, using sugar paste containing baker's yeast, was performed to understand how S. cerevisiae are introduced into honey. Apart from classical microscopy, a real-time quantitative PCR (qPCR) system specific for S. cerevisiae was established for quantification of S. cerevisiae in honeys. Results showed that S. cerevisiae cells are stored in the honey of the brood combs and are also transferred into honey in the supers...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27885812/a-whole-animal-chemical-screen-approach-to-identify-modifiers-of-intestinal-neutrophilic-inflammation
#15
Stefan H Oehlers, Maria Vega Flores, Christopher J Hall, Liuyang Wang, Dennis C Ko, Kathryn E Crosier, Philip S Crosier
By performing two high-content small molecule screens on DSS- and TNBS-induced zebrafish enterocolitis models of inflammatory bowel disease, we have identified novel anti-inflammatory drugs from the John Hopkins Clinical Compound Library that suppress neutrophilic inflammation. Live imaging of neutrophil distribution was used to assess the level of acute inflammation and concurrently screen for off-target drug effects. Supporting the validity of our screening strategy, most of the anti-inflammatory drug hits were known antibiotics or anti-inflammatory agents...
November 25, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27883365/educational-interventions-for-the-management-of-cancer-related-fatigue-in-adults
#16
REVIEW
Sally Bennett, Amanda Pigott, Elaine M Beller, Terry Haines, Pamela Meredith, Christie Delaney
BACKGROUND: Cancer-related fatigue is reported as the most common and distressing symptom experienced by patients with cancer. It can exacerbate the experience of other symptoms, negatively affect mood, interfere with the ability to carry out everyday activities, and negatively impact on quality of life. Educational interventions may help people to manage this fatigue or to cope with this symptom, and reduce its overall burden. Despite the importance of education for managing cancer-related fatigue there are currently no systematic reviews examining this approach...
November 24, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27879398/spectral-unmixing-plate-reader-high-throughput-high-precision-fret-assays-in-living-cells
#17
Tory M Schaaf, Kurt C Peterson, Benjamin D Grant, David D Thomas, Gregory D Gillispie
We have developed a microplate reader that records a complete high-quality fluorescence emission spectrum on a well-by-well basis under true high-throughput screening (HTS) conditions. The read time for an entire 384-well plate is less than 3 min. This instrument is particularly well suited for assays based on fluorescence resonance energy transfer (FRET). Intramolecular protein biosensors with genetically encoded green fluorescent protein (GFP) donor and red fluorescent protein (RFP) acceptor tags at positions sensitive to structural changes were stably expressed and studied in living HEK cells...
November 22, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27876705/genome-wide-gain-of-function-screen-for-genes-that-induce-epithelial-to-mesenchymal-transition-in-breast-cancer
#18
Dubravka Škalamera, Mareike Dahmer-Heath, Alexander J Stevenson, Cletus Pinto, Esha T Shah, Sheena M Daignault, Nur Akmarina B M Said, Melissa Davis, Nikolas K Haass, Elizabeth D Williams, Brett G Hollier, Erik W Thompson, Brian Gabrielli, Thomas J Gonda
Epithelial to mesenchymal transition (EMT) is a developmental program that has been implicated in progression, metastasis and therapeutic resistance of some carcinomas. To identify genes whose overexpression drives EMT, we screened a lentiviral expression library of 17000 human open reading frames (ORFs) using high-content imaging to quantitate cytoplasmic vimentin. Hits capable of increasing vimentin in the mammary carcinoma-derived cell line MDA-MB-468 were confirmed in the non-tumorigenic breast-epithelial cell line MCF10A...
August 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27876688/online-concerns-of-parents-suspecting-autism-spectrum-disorder-in-their-child-content-analysis-of-signs-and-automated-prediction-of-risk
#19
Ayelet Ben-Sasson, Elad Yom-Tov
BACKGROUND: Online communities are used as platforms by parents to verify developmental and health concerns related to their child. The increasing public awareness of autism spectrum disorders (ASD) leads more parents to suspect ASD in their child. Early identification of ASD is important for early intervention. OBJECTIVE: To characterize the symptoms mentioned in online queries posed by parents who suspect that their child might have ASD and determine whether they are age-specific...
November 22, 2016: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/27875504/screening-for-psychosocial-and-family-risk-in-pediatric-gastrointestinal-disorders
#20
Carin Cunningham, Elise Cho, Michele L Shaffer, Miranda C Bradford, Tonya Palermo
OBJECTIVES: Screening for psychosocial comorbidity is recommended for pediatric patients presenting at an initial gastroenterology (GI) outpatient consultation. We developed and evaluated the psychometric properties of the GI Screener to address the need for a screening tool specific to pediatric GI patients. METHODS: 128 patients (8-18 years old, 63% female) and 126 parents completed age-specific versions of the GI Screener and 3 validated psychosocial comparison instruments (The Behavioral Assessment System for Children, The Functional Disability Inventory, The General Functioning scale of the Family Assessment Device) at their initial GI consultation...
December 2016: Journal of Pediatric Gastroenterology and Nutrition
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