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https://www.readbyqxmd.com/read/28812237/pharmacological-effects-on-ceroid-lipofuscin-and-neuronal-structure-in-cln3-%C3%A2-ex7-8-mouse-brain-cultures
#1
Douglas E Brenneman, David A Pearce, Attila Kovacs, Shawn DeFrees
Juvenile Batten disease (JBD) is an inherited disorder that is characterized by the development of blindness, seizures, and progressive motor, psychiatric, and cognitive impairment. A model of JBD expressing the predominant human mutation (Cln3 (∆ex7/8) ) has been explored. Dissociated brain cultures from Cln3 (∆ex7/8) knock-in mice were compared to wild type (WT) for effects on granules of ceroid lipofuscin (CL) and neuronal structure. Utilizing high content image analysis of CL granules identified with antibodies to mitochondrial ATP synthase subunit c or tripeptidyl peptidase-1, significant increases in the areas for both immunoreactive granules were observed in Cln3 (∆ex7/8) cultures in comparison to WT...
August 15, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/28809727/models-of-non-alcoholic-fatty-liver-disease-and-potential-translational-value-the-effects-of-3-5-l-diiodothyronine
#2
Elena Grasselli, Laura Canesi, Piero Portincasa, Adriana Voci, Laura Vergani, Ilaria Demori
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in industrialized countries and is associated with increased risk of cardiovascular, hepatic and metabolic diseases. Molecular mechanisms on the root of the disrupted lipid homeostasis in NAFLD and potential therapeutic strategies can benefit of in vivo and in vitro experimental models of fatty liver. Here, we describe the high fat diet (HFD)-fed rat in vivo model, and two in vitro models, the primary cultured rat fatty hepatocytes or the FaO rat hepatoma fatty cells, mimicking human NAFLD...
August 8, 2017: Annals of Hepatology
https://www.readbyqxmd.com/read/28806985/parents-and-teachers-views-on-sexual-health-education-and-screening-for-sexually-transmitted-infections-among-in-school-adolescent-girls-in-kenya-a-qualitative-study
#3
George Wanje, Linnet Masese, Ethel Avuvika, Anisa Baghazal, Grace Omoni, R Scott McClelland
BACKGROUND: To successfully develop and implement school-based sexual health interventions for adolescent girls, such as screening for Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis, it is important to understand parents' and teachers' attitudes towards sexual health education and acceptability of sexually transmitted infection (STI) screening interventions. METHODS: In this qualitative study, we approached parents and teachers from three high schools to participate in in-depth interviews (IDIs) and focus-group discussions (FGDs)...
August 14, 2017: Reproductive Health
https://www.readbyqxmd.com/read/28805537/high-prevalence-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-wild-fish-from-the-mediterranean-sea-in-algeria
#4
Soumia Brahmi, Abdelaziz Touati, Catherine Dunyach-Remy, Albert Sotto, Alix Pantel, Jean-Philippe Lavigne
AIM: We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among wild fish from the coast of Bejaia (Algeria) in the Mediterranean Sea. RESULTS: From March 2012 to August 2013, gut and gill samples of wild fish were screened for the presence of ESBL-producing Enterobacteriaceae. Strains were characterized with regard to antibiotic resistance, β-lactamase content, plasmid-mediated quinolone resistance, aminoglycoside resistance genes, and clonality (repetitive sequence-based polymerase chain reaction profiles and multilocus sequence typing)...
August 14, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28801827/rapid-screening-and-identification-of-ace-inhibitors-in-snake-venoms-using-at-line-nanofractionation-lc-ms
#5
Marija Mladic, Tessa de Waal, Lindsey Burggraaff, Julien Slagboom, Govert W Somsen, Wilfried M A Niessen, R Manjunatha Kini, Jeroen Kool
This study presents an analytical method for the screening of snake venoms for inhibitors of the angiotensin-converting enzyme (ACE) and a strategy for their rapid identification. The method is based on an at-line nanofractionation approach, which combines liquid chromatography (LC), mass spectrometry (MS), and pharmacology in one platform. After initial LC separation of a crude venom, a post-column flow split is introduced enabling parallel MS identification and high-resolution fractionation onto 384-well plates...
August 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28801713/cross-reactivity-profiles-of-legumes-and-tree-nuts-using-the-xmap-%C3%A2-multiplex-food-allergen-detection-assay
#6
Chung Y Cho, Carolyn Oles, William Nowatzke, Kerry Oliver, Eric A E Garber
The homology between proteins in legumes and tree nuts makes it common for individuals with food allergies to be allergic to multiple legumes and tree nuts. This propensity for allergenic and antigenic cross-reactivity means that commonly employed commercial immunodiagnostic assays (e.g., dipsticks) for the detection of food allergens may not always accurately detect, identify, and quantitate legumes and tree nuts unless additional orthogonal analytical methods or secondary measures of analysis are employed...
August 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28801302/improving-patient-centered-care-for-young-people-in-general-practice-with-a-codesigned-screening-app-mixed-methods-study
#7
Marianne Julie Webb, Greg Wadley, Lena Amanda Sanci
BACKGROUND: Despite experiencing a high prevalence and co-occurrence of mental health disorders and health-compromising behaviors, young people tend not to seek professional help for these concerns. However, they do regularly attend primary care, making primary care providers ideally situated to identify and discuss mental health and lifestyle issues as part of young people's routine health care. OBJECTIVE: The aim was to investigate whether using a codesigned health and lifestyle-screening app, Check Up GP, in general practice influenced young people's assessment of the quality of their care (measures of patient-centered care and youth friendliness), and their disclosure of sensitive issues...
August 11, 2017: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/28800244/exploiting-analysis-of-heterogeneity-to-increase-the-information-content-extracted-from-fluorescence-micrographs-of-transgenic-zebrafish-embryos
#8
Tongying Shun, Albert H Gough, Subramaniam Sanker, Neil A Hukriede, Andreas Vogt
Zebrafish embryos are a near-ideal animal model for drug discovery because of their high genetic and physiological similarity to mammals, small size, high fecundity, and optical transparency. The latter properties make zebrafish at larval stages especially suited for high-content analysis and high throughput screening (HTS). However, inherent biological complexity and the inability to screen multiple specimens in a single well present a challenge for HTS because limiting replicates and high variability often prevent assays from reaching the stringent performance criteria demanded of large-scale screening assays...
August 11, 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28795916/beliefs-perceptions-and-health-seeking-behaviours-in-relation-to-cervical-cancer-a-qualitative-study-among-women-in-uganda-following-completion-of-an-hpv-vaccination-campaign
#9
Olivia Topister Hasahya, Vanja Berggren, Douglas Sematimba, Rose Chalo Nabirye, Edward Kumakech
Background Cervical cancer remains a leading cause of morbidity and mortality in Uganda. Despite earlier information campaigns to introduce human papilloma virus (HPV) vaccination, which also targeted cervical cancer, misinterpretation and misunderstanding of the subject remain high. Women in Uganda present with cervical cancer at an advanced stage due to poor health-seeking behaviours, with an associated high mortality rate. This project explored beliefs, attitudes, perceptions, and health-seeking behaviours in relation to cervical cancer among women in Uganda after an HPV vaccination project had been rolled out...
December 2016: Global Health Action
https://www.readbyqxmd.com/read/28794627/in-situ-imaging-of-quantum-dot-azd4547-conjugates-for-tracking-the-dynamic-behavior-of-fibroblast-growth-factor-receptor-3
#10
Gyoyeon Hwang, Hyeonhye Kim, Hojong Yoon, Chiman Song, Dong-Kwon Lim, Taebo Sim, Jiyeon Lee
Fibroblast growth factor receptors (FGFRs) play an important role in determining cell proliferation, differentiation, migration, and survival. Although a variety of small-molecule FGFR inhibitors have been developed for cancer therapeutics, the interaction between FGFRs and FGFR inhibitors has not been well characterized. The FGFR-inhibitor interaction can be characterized using a new imaging probe that has strong, stable signal properties for in situ cellular imaging of the interaction without quenching. We developed a kinase-inhibitor-modified quantum dot (QD) probe to investigate the interaction between FGFR and potential inhibitors...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28794014/iron-affects-ire1-clustering-propensity-and-the-amplitude-of-endoplasmic-reticulum-stress-signaling
#11
Nir Cohen, Michal Breker, Anush Bakunts, Kristina Pesek, Ainara Chas, Josepmaria Argemí, Andrea Orsi, Lihi Gal, Silvia Chuartzman, Yoav Wigelman, Felix Jonas, Peter Walter, Robert Ernst, Tomás Aragón, Eelco van Anken, Maya Schuldiner
The unfolded protein response (UPR) allows cells to adjust secretory pathway capacity according to need. Ire1, the endoplasmic reticulum (ER) stress sensor and central activator of the UPR is conserved from the budding yeast Saccharomyces cerevisiae to humans. Under ER stress conditions, Ire1 clusters into foci that enable optimal UPR activation. To discover factors that affect Ire1 clustering, we performed a high-content screen using a whole-genome yeast mutant library expressing Ire1-mCherry. We imaged the strains following UPR induction and found 154 strains that displayed alterations in Ire1 clustering...
August 9, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28790022/usability-testing-of-the-branch-smartphone-app-designed-to-reduce-harmful-drinking-in-young-adults
#12
Joanna Milward, Paolo Deluca, Colin Drummond, Rod Watson, Jacklyn Dunne, Andreas Kimergård
BACKGROUND: Electronic screening and brief intervention (eSBI) apps demonstrate potential to reduce harmful drinking. However, low user engagement rates with eSBI reduce overall effectiveness of interventions. As "Digital Natives," young adults have high expectations of app quality. Ensuring that the design, content, and functionality of an eSBI app are acceptable to young adults is an integral stage to the development process. OBJECTIVE: The objective of this study was to identify usability barriers and enablers for an app, BRANCH, targeting harmful drinking in young adults...
August 8, 2017: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/28789994/high-content-analysis-of-phagocytic-activity-and-cell-morphology-with-puntomorph
#13
Hassan Al-Ali, Han Gao, Camilla Dalby-Hansen, Vanessa Ann Peters, Yan Shi, Roberta Brambilla
BACKGROUND: Phagocytosis is essential for maintenance of normal homeostasis and healthy tissue. As such, it is a therapeutic target for a wide range of clinical applications. The development of phenotypic screens targeting phagocytosis has lagged behind, however, due to the difficulties associated with image-based quantification of phagocytic activity. NEW METHOD: We present a robust algorithm and cell-based assay system for high content analysis of phagocytic activity...
August 5, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28783636/unified-blind-quality-assessment-of-compressed-natural-graphic-and-screen-content-images
#14
Xiongkuo Min, Kede Ma, Ke Gu, Guangtao Zhai, Zhou Wang, Weisi Lin
Digital images in the real-world are created by a variety of means and have diverse properties. A photographical natural scene image (NSI) may exhibit substantially different characteristics from a computer graphic image (CGI) or a screen content image (SCI). This casts major challenges to objective image quality assessment, for which existing approaches lack effective mechanisms to capture such content type variations, and thus are difficult to generalize from one type to another. To tackle this problem, we first construct a cross-content-type (CCT) database which contains 1,320 distorted NSIs, CGIs, and SCIs, compressed using the high efficiency video coding (HEVC) intra coding method and the screen content compression (SCC) extension of HEVC...
August 2, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28783477/a-high-content-live-cell-viability-assay-and-its-validation-on-a-diverse-12k-compound-screen
#15
Jeanne Chiaravalli, J Fraser Glickman
We have developed a new high-content cytotoxicity assay using live cells, called "ImageTOX." We used a high-throughput fluorescence microscope system, image segmentation software, and the combination of Hoechst 33342 and SYTO 17 to simultaneously score the relative size and the intensity of the nuclei, the nuclear membrane permeability, and the cell number in a 384-well microplate format. We then performed a screen of 12,668 diverse compounds and compared the results to a standard cytotoxicity assay. The ImageTOX assay identified similar sets of compounds to the standard cytotoxicity assay, while identifying more compounds having adverse effects on cell structure, earlier in treatment time...
August 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28775673/the-image-data-resource-a-bioimage-data-integration-and-publication-platform
#16
Eleanor Williams, Josh Moore, Simon W Li, Gabriella Rustici, Aleksandra Tarkowska, Anatole Chessel, Simone Leo, Bálint Antal, Richard K Ferguson, Ugis Sarkans, Alvis Brazma, Rafael E Carazo Salas, Jason R Swedlow
Access to primary research data is vital for the advancement of science. To extend the data types supported by community repositories, we built a prototype Image Data Resource (IDR) that collects and integrates imaging data acquired across many different imaging modalities. IDR links data from several imaging modalities, including high-content screening, super-resolution and time-lapse microscopy, digital pathology, public genetic or chemical databases, and cell and tissue phenotypes expressed using controlled ontologies...
August 2017: Nature Methods
https://www.readbyqxmd.com/read/28771372/high-content-assay-multiplexing-for-vascular-toxicity-screening-in-induced-pluripotent-stem-cell-derived-endothelial-cells-and-human-umbilical-vein-endothelial-cells
#17
Yasuhiro Iwata, William D Klaren, Connie S Lebakken, Fabian A Grimm, Ivan Rusyn
Endothelial cells (ECs) play a major role in blood vessel formation and function. While there is longstanding evidence for the potential of chemical exposures to adversely affect EC function and vascular development, the hazard potential of chemicals with respect to vascular effects is not routinely evaluated in safety assessments. Induced pluripotent stem cell (iPSC)-derived ECs promise to provide a physiologically relevant, organotypic culture model that is amenable for high-throughput (HT) EC toxicant screening and may represent a viable alternative to traditional in vitro models, including human umbilical vein endothelial cells (HUVECs)...
August 3, 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28770923/exploring-the-upper-limit-of-single-walled-carbon-nanotube-purity-by-multiple-cycle-aqueous-two-phase-separation
#18
Li Wei, Benjamin S Flavel, Wenshan Li, Ralph Krupke, Yuan Chen
Ultrahigh purity semiconducting single-walled carbon nanotubes (S-SWCNTs) are required for high-performance transistors. Aqueous two-phase (ATP) separation is an attractive method to obtain such SWCNTs due to its simplicity and scalability. This work targeted two questions; namely what is the upper limit of S-SWCNT purity that can be achieved by multiple cycles of ATP separation from the most commonly used polyethylene glycol and dextran system and how accurately can commonly used methods characterize the improvement in purity? SWCNT purity in nanotube dispersions obtained by multi-cycle ATP separation (2, 4, 6 and 8 cycles) was evaluated by three methods, including UV-vis-NIR absorption spectroscopy analysis, performance of thin-film field effect transistors (FETs) prepared by drop casting and short-channel FET devices prepared by dielectrophoresis deposition...
August 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28769790/phenotypic-screening-identifies-synergistically-acting-natural-product-enhancing-the-performance-of-biomaterial-based-wound-healing
#19
Srinivasan Sivasubramanian, Gayathri Chandrasekar, Sara Svensson Akusjärvi, Ramar Thangam, Malairaj Sathuvan, R B S Kumar, Hawraa Hussein, Savariar Vincent, Balaraman Madhan, Palani Gunasekaran, Satish S Kitambi
The potential of multifunctional wound heal biomaterial relies on the optimal content of therapeutic constituents as well as the desirable physical, chemical, and biological properties to accelerate the healing process. Formulating biomaterials such as amnion or collagen based scaffolds with natural products offer an affordable strategy to develop dressing material with high efficiency in healing wounds. Using image based phenotyping and quantification, we screened natural product derived bioactive compounds for modulators of types I and III collagen production from human foreskin derived fibroblast cells...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28769026/chemical-composition-antioxidant-anti-inflammatory-and-antiproliferative-activities-of-the-plant-lebanese-crataegus-azarolus-l
#20
Hany Kallassy, Mohammad Fayyad-Kazan, Rawan Makki, Yolla El-Makhour, Eva Hamade, Hasan Rammal, David Y Leger, Vincent Sol, Hussein Fayyad-Kazan, Bertrand Liagre, Bassam Badran
BACKGROUND In the present study, phytochemical screening, antioxidant, anti-inflammatory, and antiproliferative capacities of 3 extracts from leaves of Lebanese Crataegus azarolus L. were evaluated. MATERIAL AND METHODS Fresh leaves were dissolved in 3 different solvents: distilled water, ethanol, and methanol. The chemical composition was determined using high-performance liquid chromatography (HPLC) and the content of essential oil of this plant was examined by gas chromatography (GC) coupled with mass spectrometry (MS)...
August 3, 2017: Medical Science Monitor Basic Research
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