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https://www.readbyqxmd.com/read/28531129/rapid-and-cost-effective-quantification-of-glucosinolates-and-total-phenolic-content-in-rocket-leaves-by-visible-near-infrared-spectroscopy
#1
Eva María Toledo-Martín, Rafael Font, Sara Obregón-Cano, Antonio De Haro-Bailón, Myriam Villatoro-Pulido, Mercedes Del Río-Celestino
The potential of visible-near infrared spectroscopy to predict glucosinolates and total phenolic content in rocket (Eruca vesicaria) leaves has been evaluated. Accessions of the E. vesicaria species were scanned by NIRS as ground leaf, and their reference values regressed against different spectral transformations by modified partial least squares (MPLS) regression. The coefficients of determination in the external validation (R²VAL) for the different quality components analyzed in rocket ranged from 0.59 to 0...
May 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28530605/screening-of-passiflora-l-mineral-content-using-principal-component-analysis-and-kohonen-self-organizing-maps
#2
Cleber Galvão Novaes, Ivana Lago da Silva Romão, Berlane Gomes Santos, Jéssica Palhares Ribeiro, Marcos Almeida Bezerra, Erik Galvão Paranhos da Silva
A method using digestion with diluted nitric acid and inductively coupled plasma optical emission spectrometry was applied to determine metal ions in the peel, pulp, and seed of Passiflora edulis f. flavicarpa and Passiflora cincinnata cultivated in the Bahia state, Brazil. Plasma parameters (radio frequency power and argon flow rates) were optimized. The quantification limits (µg g(-1)) were: Ca (43.0); Co (1.3); Cu (10.0); Fe (10.0); K (20.0); Mg (26.6); Mn (0.33); Na (56.6) and Zn (10.0). The accuracy of the method was evaluated by the analysis of certified reference materials (NIST 1567a Wheat Flour and NIST 1577b Bovine Liver)...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28523436/hrm-and-snapshot-as-alternative-forensic-snp-genotyping-methods
#3
Bhavik Mehta, Runa Daniel, Dennis McNevin
Single nucleotide polymorphisms (SNPs) have been widely used in forensics for prediction of identity, biogeographical ancestry (BGA) and externally visible characteristics (EVCs). Single base extension (SBE) assays, most notably SNaPshot® (Thermo Fisher Scientific), are commonly used for forensic SNP genotyping as they can be employed on standard instrumentation in forensic laboratories (e.g. capillary electrophoresis). High resolution melt (HRM) analysis is an alternative method and is a simple, fast, single tube assay for low throughput SNP typing...
May 18, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28518086/ptr-tof-ms-coupled-with-an-automated-sampling-system-and-tailored-data-analysis-for-food-studies-bioprocess-monitoring-screening-and-nose-space-analysis
#4
Vittorio Capozzi, Sine Yener, Iuliia Khomenko, Brian Farneti, Luca Cappellin, Flavia Gasperi, Matteo Scampicchio, Franco Biasioli
Proton Transfer Reaction (PTR), combined with a Time-of-Flight (ToF) Mass Spectrometer (MS) is an analytical approach based on chemical ionization that belongs to the Direct-Injection Mass Spectrometric (DIMS) technologies. These techniques allow the rapid determination of volatile organic compounds (VOCs), assuring high sensitivity and accuracy. In general, PTR-MS requires neither sample preparation nor sample destruction, allowing real time and non-invasive analysis of samples. PTR-MS are exploited in many fields, from environmental and atmospheric chemistry to medical and biological sciences...
May 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28512456/proline-based-cyclic-dipeptides-from-korean-fermented-vegetable-kimchi-and-from-leuconostoc-mesenteroides-lbp-k06-have-activities-against-multidrug-resistant-bacteria
#5
Rui Liu, Andrew H Kim, Min-Kyu Kwak, Sa-Ouk Kang
Lactobacillus plantarum and Leuconostoc mesenteroides play a prominent role as functional starters and predominant isolates in the production of various types of antimicrobial compound-containing fermented foods, especially including kimchi. In the case of the bioactive cyclic dipeptides, their racemic diastereomers inhibitory to bacteria and fungi have been suggested to come solely from Lactobacillus spp. of these strains. We previously demonstrated the antifungal and antiviral activities of proline-based cyclic dipeptides, which were fractionated from culture filtrates of Lb...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28511186/search-for-internal-cancers-in-mice-tattooed-with-inks-of-high-contents-of-potential-carcinogens-a-one-year-autopsy-study-of-red-and-black-tattoo-inks-banned-in-the-market
#6
Mitra Sepehri, Catharina M Lerche, Katrina Hutton Carlsen, Jørgen Serup
BACKGROUND AND OBJECTIVE: Tattoo ink stock products often contain potential carcinogens, which on large-scale population exposure may be clinically relevant. The aim of this autopsy study in mice was to screen major organs for clinical and subclinical cancers. METHODS: Mice were tattooed on their backs. In total, 48 mice were included and divided into 4 groups; 11 mice tattooed black, 10 tattooed red, and 5 mice serving as untreated controls. A group of 22 mice with black tattoos and exposed to ultraviolet radiation (UVR) were also studied...
May 17, 2017: Dermatology: International Journal for Clinical and Investigative Dermatology
https://www.readbyqxmd.com/read/28510247/characterization-and-development-of-56-est-ssr-markers-derived-from-the-transcriptome-of-odontobutis-potamophila
#7
P P Wang, M Ren, S Q Chen, S W Yin, C Zhao, H Y Zhang, X R Li, Q Q Cao, G Q Zhou
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two individuals from a wild population area (Jiande, Zhejiang Province, China). The number of alleles per locus varied from two to eight, the observed heterozygosity (HO) ranged from 0...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28505176/systematic-multiparametric-analysis-of-mycobacterium-tuberculosis-intracellular-infection-offers-insight-into-coordinated-virulence
#8
Amy K Barczak, Roi Avraham, Shantanu Singh, Samantha S Luo, Wei Ran Zhang, Mark-Anthony Bray, Amelia E Hinman, Matthew Thompson, Raymond M Nietupski, Aaron Golas, Paul Montgomery, Michael Fitzgerald, Roger S Smith, Dylan W White, Anna D Tischler, Anne E Carpenter, Deborah T Hung
A key to the pathogenic success of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is the capacity to survive within host macrophages. Although several factors required for this survival have been identified, a comprehensive knowledge of such factors and how they work together to manipulate the host environment to benefit bacterial survival are not well understood. To systematically identify Mtb factors required for intracellular growth, we screened an arrayed, non-redundant Mtb transposon mutant library by high-content imaging to characterize the mutant-macrophage interaction...
May 15, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28501082/a-high-content-screening-technology-for-quantitatively-studying-podocyte-dynamics
#9
REVIEW
Jochen Reiser, Ha Won Lee, Vineet Gupta, Mehmet M Altintas
Podocytes form the visceral layer of a kidney glomerulus and express a characteristic octopus-like cellular architecture specialized for the ultrafiltration of blood. The cytoskeletal dynamics and structural elasticity of podocytes rely on the self-organization of highly interconnected actin bundles, and the maintenance of these features is important for the intact glomerular filtration. Development of more differentiated podocytes in culture has dramatically increased our understanding of the molecular mechanisms regulating podocyte actin dynamics...
May 2017: Advances in Chronic Kidney Disease
https://www.readbyqxmd.com/read/28500405/est-ssr-marker-revealed-effective-over-biochemical-and-morphological-scepticism-towards-identification-of-specific-turmeric-curcuma-longa-l-cultivars
#10
Ambika Sahoo, Sudipta Jena, Basudeba Kar, Suprava Sahoo, Asit Ray, Subhashree Singh, Raj Kumar Joshi, Laxmikanta Acharya, Sanghamitra Nayak
Turmeric (Curcuma longa L., family Zingiberaceae) is one of the most economically important plants for its use in food, medicine, and cosmetic industries. Cultivar identification is a major constraint in turmeric, owing to high degree of morphological similarity that in turn, affects its commercialization. The present study addresses this constraint, using EST-SSR marker based, molecular identification of 8 elite cultivars and 88 accessions in turmeric. Fifty EST-SSR primers were screened against eight cultivars of turmeric (Suroma, Roma, Lakadong, Megha, Alleppey Supreme, Kedaram, Pratibha, and Suvarna); out of which 11 primers showed polymorphic banding pattern...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28498012/unmet-expectations-of-medications-and-care-providers-among-patients-with-heart-failure-assessed-to-be-poorly-adherent-results-from-the-chronic-heart-failure-intervention-to-improve-medication-adherence-chime-study
#11
Inger Ekman, Axel Wolf, Victoria Vaughan Dickson, Hayden B Bosworth, Bradi B Granger
BACKGROUND: Ineffective medication management contributes to repeated hospitalisation and death among patients with heart failure. The meaning ascribed to medications and the influence of meaning on how patients manage medications is unknown. The purpose of this study was to explore the meaning and expectations associated with medication use in high-risk, non-adherent patients with heart failure. METHODS AND RESULTS: Patients ( n=265) with heart failure were screened for adherence to prescribed medication using the Morisky medication adherence scale (MMAS)...
May 1, 2017: European Journal of Cardiovascular Nursing
https://www.readbyqxmd.com/read/28497952/generalized-subset-designs-in-analytical-chemistry
#12
Izabella Maria Surowiec, Ludvig Vikström, Gustaf Hector, Erik Johansson, Conny Vikström, Johan Trygg
Design of experiments (DOE) is an established methodology in research, development, manufacturing and production for screening, optimization and robustness testing. Two level fractional factorial designs remain the preferred approach due to high information content while keeping the number of experiments low. These types of designs, however, have never been extended to a generalized multi-level reduced design type that would be capable to include both qualitative and quantitative factors. In this paper we describe a novel generalized fractional factorial design...
May 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28494853/in-vitro-antioxidant-assessment-and-a-rapid-hptlc-bioautographic-method-for-the-detection-of-anticholinesterase-inhibitory-activity-of-geophila-repens
#13
Umesh Chandra Dash, Atish Kumar Sahoo
OBJECTIVE: Geophila repens (L.) I. M. Johnst. (Rubiaceae), a small, creeping, perennial herb, is claimed to have memory-enhancing property. The goal of this study was to assess its antioxidant and anticholinesterase activity and conduct a rapid bioautographic enzyme assay for screening acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition of G. repens extracts. METHODS: Antioxidant activity of G. repens extracts was assessed by performing 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), superoxide (SOD), hydroxyl (OH) and total antioxidant capacity (TAC) assays...
May 2017: Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28494548/-the-role-of-cytochrome-p450-in-nonalcoholic-fatty-liver-induced-by-high-fat-diet-a-gene-expression-profile-analysis
#14
Y Liu, F Cheng, Y X Luo, P Hu, H Ren, M L Peng
Objective: To clarify the role of cytochrome P450 in nonalcoholic fatty liver disease (NAFLD) by RNA-Seq and bioinformatics analysis. Methods: A total of 20 male C57BL/6 mice were used. Ten mice were fed with high-fat diet (D12492, 60% kcal fat) for 16 weeks to establish a mouse model of NAFLD, and the other 10 mice were fed with low-fat diet (D12450B, 10% kcal fat) as control group. At the end of the experiment, the body weight, liver weight, and hepatic triglyceride (TG) content were measured. Meanwhile, HE staining and RNA-Seq analysis were performed for the liver tissues...
April 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28490437/high-throughput-crispri-phenotyping-identifies-new-essential-genes-in-streptococcus-pneumoniae
#15
Xue Liu, Clement Gallay, Morten Kjos, Arnau Domenech, Jelle Slager, Sebastiaan P van Kessel, Kèvin Knoops, Robin A Sorg, Jing-Ren Zhang, Jan-Willem Veening
Genome-wide screens have discovered a large set of essential genes in the opportunistic human pathogen Streptococcus pneumoniae However, the functions of many essential genes are still unknown, hampering vaccine development and drug discovery. Based on results from transposon sequencing (Tn-seq), we refined the list of essential genes in S. pneumoniae serotype 2 strain D39. Next, we created a knockdown library targeting 348 potentially essential genes by CRISPR interference (CRISPRi) and show a growth phenotype for 254 of them (73%)...
May 10, 2017: Molecular Systems Biology
https://www.readbyqxmd.com/read/28484750/in-vitro-field-potential-monitoring-on-a-multi-microelectrode-array-for-the-electrophysiological-long-term-screening-of-neural-stem-cell-maturation
#16
Diana Seidel, Heinz-Georg Jahnke, Beate Englich, Mathilde Girard, Andrea A Robitzki
Due to the lack of appropriate cell models as well as automated electrophysiology monitoring technologies, the standardized identification of neurotoxic or protective effects in vitro remains a major problem in today's pharmaceutical ingredient development. Over the past few years, in vivo-like human pluripotent stem cell-derived neuronal networks have turned out to be a promising physiological cell source, if the establishment of robust and time-saving functional maturation strategies based on stable and expandable neural progenitor populations can be achieved...
May 9, 2017: Analyst
https://www.readbyqxmd.com/read/28477112/image-based-high-content-screening-automating-the-quantification-process-for-dna-damage-induced-foci
#17
Yi Chieh Lim
Visual inspection of cellular activities based on conventional fluorescence microscope is a fundamental tool to study the role of DNA damage response (DDR). In the context of drug discovery where the capture of thousands of images is required across parallel experiments, this presents a challenge to data collection and analysis. Manual scoring is laborious and often reliant on trained personnel to intuit biological meaning through visual reasoning. On the other hand, high content screening combines the automation of microscopy image acquisition and analysis in a single platform to quantify cellular events of interests...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28477071/discrimination-and-support-from-friends-and-family-members-experienced-by-people-with-mental-health-problems-findings-from-an-australian-national-survey
#18
Amy J Morgan, N J Reavley, A F Jorm, R Beatson
PURPOSE: To investigate the scope and nature of discrimination and positive treatment experienced by adults with mental health problems from their friends and family in a population-based survey. METHODS: An Australian telephone-survey of 5220 adults included 1381 individuals who reported a mental health problem or scored high on a screening questionnaire. Respondents were interviewed about their experience of discrimination and positive treatment from their friends, spouse and other family members...
May 5, 2017: Social Psychiatry and Psychiatric Epidemiology
https://www.readbyqxmd.com/read/28476626/nile-red-fluorescence-spectrum-decomposition-enables-rapid-screening-of-large-protein-aggregates-in-complex-biopharmaceutical-formulations-like-influenza-vaccines
#19
Ziya Sahin, Senem Akkoc, Ronald Neeleman, Jonathan Haines, Veysel Kayser
The extensive presence of large (high molecular weight) protein aggregates in biopharmaceutical formulations is a concern for formulation stability and possibly safety. Tests to screen large aggregate content in such bioformulations are therefore needed for rapid and reliable quality control in industrial settings. Herein, non-commercial seasonal influenza split-virus vaccine samples, produced using various strains and extracted from selected industrial processing steps, were used as model complex bioformulations...
May 2, 2017: Vaccine
https://www.readbyqxmd.com/read/28476101/community-based-randomized-controlled-trial-of-diabetes-prevention-study-for-high-risk-individuals-of-type-2-diabetes-lifestyle-intervention-using-web-based-system
#20
Seon-Ah Cha, Sun-Young Lim, Kook-Rye Kim, Eun-Young Lee, Borami Kang, Yoon-Hee Choi, Kun-Ho Yoon, Yu-Bae Ahn, Jin-Hee Lee, Seung-Hyun Ko
BACKGROUND: The trend of increasing numbers of patients with type 2 diabetes emphasizes the need for active screening of high-risk individuals and intensive lifestyle modification (LSM). METHODS/DESIGN: The community-based Korean Diabetes Prevention Study (C-KDPS) is a randomized controlled clinical trial to prevent type 2 diabetes by intensive LSM using a web-based program. The two public healthcare centers in Korea are involved, and 420 subjects are being recruited for 6 months and will be followed up for 22 months...
May 5, 2017: BMC Public Health
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