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https://www.readbyqxmd.com/read/29158634/aluminum-exclusion-from-root-zone-and-maintenance-of-nutrient-uptake-are-principal-mechanisms-of-al-tolerance-in-pisum-sativum-l
#1
Natalia E Kichigina, Jan V Puhalsky, Aleksander I Shaposhnikov, Tatiana S Azarova, Natalia M Makarova, Svyatoslav I Loskutov, Vera I Safronova, Igor A Tikhonovich, Margarita A Vishnyakova, Elena V Semenova, Irina A Kosareva, Andrey A Belimov
Our study aimed to evaluate intraspecific variability of pea (Pisum sativum L.) in Al tolerance and to reveal mechanisms underlying genotypic differences in this trait. At the first stage, 106 pea genotypes were screened for Al tolerance using root re-elongation assay based on staining with eriochrome cyanine R. The root re-elongation zone varied from 0.5 mm to 14 mm and relationships between Al tolerance and provenance or phenotypic traits of genotypes were found. Tolerance index (TI), calculated as a biomass ratio of Al-treated and non-treated contrasting genotypes grown in hydroponics for 10 days, varied from 30% to 92% for roots and from 38% to 90% for shoots...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29157415/attention-in-action-and-perception-unitary-or-separate-mechanisms-of-selectivity
#2
James T Enns, Allison A Brennan, Robert L Whitwell
What is the relation between the two visual stream hypothesis and selective visual attention? In this chapter, we first consider this question at a theoretical level before presenting an example of work from our lab that examines the question: Under what conditions does the emotional content of a visual object influence visually guided action? Previous research has demonstrated that fear can influence perception, both consciously and unconsciously, but it is unclear when fear influences visually guided action...
2017: Progress in Brain Research
https://www.readbyqxmd.com/read/29145931/in-vitro-and-in-silico-approaches-to-study-cytochrome-p450-mediated-interactions
#3
Boon Hooi Tan, Yan Pan, Amelia Nathania Dong, Chin Eng Ong
In vitro and in silico models of drug metabolism are utilized regularly in the drug research and development as tools for assessing pharmacokinetic variability and drug-drug interaction risk. The use of in vitro and in silico predictive approaches offers advantages including guiding rational design of clinical drug-drug interaction studies, minimization of human risk in the clinical trials, as well as cost and time savings due to lesser attrition during compound development process. This article gives a review of some of the current in vitro and in silico methods used to characterize cytochrome P450(CYP)-mediated drug metabolism for estimating pharmacokinetic variability and the magnitude of drug-drug interactions...
2017: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29145487/defining-external-factors-that-determine-neuronal-survival-apoptosis-and-necrosis-during-excitotoxic-injury-using-a-high-content-screening-imaging-platform
#4
Ujval Anilkumar, Petronela Weisova, Jasmin Schmid, Tytus Bernas, Heinrich J Huber, Heiko Düssmann, Niamh M C Connolly, Jochen H M Prehn
Cell death induced by excessive glutamate receptor overactivation, excitotoxicity, has been implicated in several acute and chronic neurological disorders. While numerous studies have demonstrated the contribution of biochemically and genetically activated cell death pathways in excitotoxic injury, the factors mediating passive, excitotoxic necrosis are less thoroughly investigated. To address this question, we developed a high content screening (HCS) based assay to collect high volumes of quantitative cellular imaging data and elucidated the effects of intrinsic and external factors on excitotoxic necrosis and apoptosis...
2017: PloS One
https://www.readbyqxmd.com/read/29143866/haplotype-analysis-of-the-germacrene-a-synthase-gene-and-association-with-cynaropicrin-content-and-biological-activities-in-cynara-cardunculus
#5
Ana Margarida Ferro, Patrícia Ramos, Ângela Guerra, Paula Parreira, Teresa Brás, Olinda Guerreiro, Eliana Jerónimo, Carmen Capel, Juan Capel, Fernando J Yuste-Lisbona, Maria F Duarte, Rafael Lozano, M Margarida Oliveira, Sónia Gonçalves
Cynara cardunculus: L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of this work was to screen a collection of C. cardunculus, from different origins, for new allelic variants in germacrene A synthase (GAS) gene involved in the cynaropicrin biosynthesis and correlate them with improved cynaropicrin content and biological activities...
November 16, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29143601/morphological-description-and-dna-barcoding-study-of-sand-rice-agriophyllum-squarrosum-chenopodiaceae-collected-in-kazakhstan
#6
Yuliya Genievskaya, Saule Abugalieva, Aibatsha Zhubanysheva, Yerlan Turuspekov
BACKGROUND: Sand rice (Agriophyllum squarrosum (L.) Moq.) is an annual shrub-like plant adapted to the mobile sand dunes in desert and semi-desert regions of Asia. It has a balanced nutrient composition with relatively high concentration of lipids and proteins, which results in its nutrition being similar to legumes. Sand rice's proteins contain the full range of essential amino acids. However, calories content is more similar to wheat. These features together with desert stress resistance make sand rice a potential food crop resilient to ongoing climate change...
November 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29143146/screening-transferable-microsatellite-markers-across-genus-phalaenopsis-orchidaceae
#7
Ya-Zhu Ko, Huei-Chuan Shih, Chi-Chu Tsai, Hsing-Hua Ho, Pei-Chun Liao, Yu-Chung Chiang
BACKGROUND: Molecular identification based on microsatellite loci is an important technology to improve the commercial breeding of the moth orchid. There are more than 30,000 cultivars have been enrolled at the Royal Horticultural Society (RHS). In this study, genomic microsatellite primer sets were developed from Phalaenopsis aphrodite subsp. formosana to further examine the transferability of across 21 Phalaenopsis species. METHODS AND RESULTS: Twenty-eight polymorphic microsatellite markers were obtained using the magnetic bead enrichment method, with high transferability of the 21 species of the genus Phalaenopsis, especially in the subgenus Phalaenopsis...
November 15, 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/29142439/screening-of-norharmane-from-seven-cyanobacteria-by-high-performance-liquid-chromatography
#8
Tunay Karan, Ramazan Erenler
Background: Cyanobacteria, including pharmaceutically and medicinally valuable compounds attract the great attention lately. Norharmane (9H-pyrido (3,4-b) indole found in some cyanobacteria revealed a great number of biological effects. Objective: Seven cyanobacteria were isolated and identified from Yesilirmak River and Gaziosmanpasa University Campus to determine the norharmane content. Materials and Methods: Cyanobacteria collected from Tokat, Turkey were isolated and identified by morphologically...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142429/antioxidant-and-hepatoprotective-potential-of-swaras-and-hima-extracts-of-tinospora-cordifolia-and-boerhavia-diffusa-in-swiss-albino-mice
#9
Amit Kaushik, Athar Husain, Harshika Awasthi, Dewasya Pratap Singh, Raziuddin Khan, Dayanandan Mani
Background: In Ayurveda, five basic extraction procedures are mentioned in order of their decreasing potency. Swaras is considered as the most potent followed by, kalka, kwatha, fanta and hima. Objective: Present study was carried out to investigate the antioxidant and hepatoprotective potential of swaras and hima extracts of T.cordifolia and B. diffusa. Materials and Methods: Swaras and hima extracts of T. cordifolia and B. diffusa were prepared...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142414/growth-arresting-activity-of-acmella-essential-oil-and-its-isolated-component-d-limonene-1-8-p-mentha-diene-against-trichophyton-rubrum-microbial-type-culture-collection-296
#10
Diptikanta Padhan, Smaranika Pattnaik, Ajaya Kumar Behera
Background: Spilanthes acmella is used as a remedy in toothache complaints by the tribal people of Western part of Odisha, India. Objective: The objective of this study was to study the growth-arresting activity of an indigenous Acmella essential oil (EO) (S. acmella Murr, Asteraceae) and its isolated component, d-limonene against Trichophyton rubrum (microbial type culture collection 296). Materials and Methods: The EO was extracted from flowers of indigenous S...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29136861/quality-by-design-approach-in-the-development-of-an-ultra-high-performance-liquid-chromatography-method-for-bexsero-meningococcal-group-b-vaccine
#11
Luca Nompari, Serena Orlandini, Benedetta Pasquini, Cristiana Campa, Michele Rovini, Massimo Del Bubba, Sandra Furlanetto
Bexsero is the first approved vaccine for active immunization of individuals from 2 months of age and older to prevent invasive disease caused by Neisseria meningitidis serogroup B. The active components of the vaccine are Neisseria Heparin Binding Antigen, factor H binding protein, Neisseria adhesin A, produced in Escherichia coli cells by recombinant DNA technology, and Outer Membrane Vesicles (expressing Porin A and Porin B), produced by fermentation of Neisseria meningitidis strain NZ98/254. All the Bexsero active components are adsorbed on aluminum hydroxide and the unadsorbed antigens content is a product critical quality attribute...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29132906/a-selective-knockout-method-for-discovery-of-minor-active-components-from-plant-extracts-feasibility-and-challenges-as-illustrated-by-an-application-to-salvia-miltiorrhiza
#12
Xue-Yan Li, Hong-Jin Tang, Liu Zhang, Lin Yang, Ping Li, Jun Chen
Natural products have been recognized to play an invaluable role in drug discovery. However, efficient discovery of minor active constituents from natural sources is challenging due to the low abundance and complex matrices. In this study, we developed a selective knockout method to discover minor bioactive components from complex phytochemical mixtures, using a Chinese medicine as an example. Based on the chromatographic fingerprint, six major components in the ethyl acetate extract of the root of Salvia miltiorrhiza (EASM) were selectively knocked out via high-resolution peak fraction (HRPF) approach...
October 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29132358/ftir-spectroscopy-as-a-unified-method-for-simultaneous-analysis-of-intra-and-extracellular-metabolites-in-high-throughput-screening-of-microbial-bioprocesses
#13
Gergely Kosa, Volha Shapaval, Achim Kohler, Boris Zimmermann
BACKGROUND: Analyses of substrate and metabolites are often bottleneck activities in high-throughput screening of microbial bioprocesses. We have assessed Fourier transform infrared spectroscopy (FTIR), in combination with high throughput micro-bioreactors and multivariate statistical analyses, for analysis of metabolites in high-throughput screening of microbial bioprocesses. In our previous study, we have demonstrated that high-throughput (HTS) FTIR can be used for estimating content and composition of intracellular metabolites, namely triglyceride accumulation in oleaginous filamentous fungi...
November 13, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29131641/qcm-d-investigation-of-swelling-behavior-of-layer-by-layer-thin-films-upon-exposure-to-monovalent-ions
#14
Joshua T O'Neal, Ethan Y Dai, Yanpu Zhang, Kyle B Clark, Kathryn G Wilcox, Ian M George, Nandha E Ramasamy, Daisy Enriquez, Piotr Batys, Maria Sammalkorpi, Jodie L Lutkenhaus
Polyelectrolyte multilayers and layer-by-layer assemblies are susceptible to structural changes in response to ionic environment. By altering the salt type and ionic strength, structural changes can be induced by disruption of intrinsically bound ion pairs within the multilayer network via electrostatic screening. Notably, high salt concentrations have been used for the purposes of salt-annealing and self-healing of LbL assemblies with KBr, in particular, yielding a remarkably rapid response. However, to date, the structural and swelling effects of various monovalent ion species on the behavior of LbL assemblies remain unclear, including a quantitative view of ion content in the LbL assembly and thickness changes over a wide concentration window...
November 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29131211/pharmaco-genomic-investigations-of-organo-iridium-anticancer-complexes-reveal-novel-mechanism-of-action
#15
Jessica M Hearn, George M Hughes, Isolda Romero-Canelón, Alison F Munro, Belén Rubio-Ruiz, Zhe Liu, Neil O Carragher, Peter J Sadler
Resistance to platinum drugs (used in >50% of cancer chemotherapies) is a clinical problem. Other precious metal complexes with distinct mechanisms of action might overcome this. Half-sandwich organometallic complexes containing arene or cyclopentadienyl (Cp) ligands show promise. We screened two iridium(iii) complexes [Ir(Cp(Xbiph))(ppy)Cl] (ZL49, 1, ppy = phenylpyridine) and [Ir(Cp(Xph))(azpyNMe2)Cl]PF6 (ZL109, 2, azpyNMe2 = N,N-dimethylphenylazopyridine) in 916 cancer cell lines from 28 tissue types. On average, complex 2 was 78× more potent than 1, 36× more active than cisplatin (CDDP), and strongly active (nanomolar) in patient-derived ovarian cancer cell lines...
November 13, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29130161/detection-of-2-alkyl-4-quinolones-using-biosensors
#16
Matthew P Fletcher, Stephen P Diggle, Miguel Cámara, Paul Williams
2-Alkyl-4-quinolones (AQs) such as 2-heptyl-3-hydroxy-4-quinolone (PQS) and 2-heptyl-4-hydroxyquinoline (HHQ) are quorum-sensing signal molecules. Here we describe two methods for AQ detection and quantification that employ thin-layer chromatography (TLC) and microtiter plate assays in combination with a lux-based Pseudomonas aeruginosa AQ biosensor strain. For TLC detection, organic solvent extracts of bacterial cells or spent culture supernatants are chromatographed on TLC plates, which are then dried and overlaid with the AQ biosensor...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29128994/radiologists-preferences-regarding-content-of-prostate-mri-reports-a-survey-of-the-society-of-abdominal-radiology
#17
Benjamin Spilseth, Daniel J Margolis, Sangeet Ghai, Nayana U Patel, Andrew B Rosenkrantz
PURPOSE: To evaluate radiologist preferences regarding specific content that warrants inclusion in prostate MRI reports. METHODS: Sixty-one members of the Society of Abdominal Radiology responded to a 74-item survey regarding specific content warranted in prostate MRI reports, conducted in August 2016. RESULTS: General items deemed essential report content by ≥ 50% of respondents were prostate volume (80%), extent of prostate hemorrhage (74%), TURP defects (69%), coil type (64%), BPH (61%), contrast dose (61%), contrast agent (59%), medications administered (59%), and magnet strength (54%)...
November 11, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/29125598/a-mir-20a-mapk1-c-myc-regulatory-feedback-loop-regulates-breast-carcinogenesis-and-chemoresistance
#18
Wengong Si, Jiaying Shen, Chengyong Du, Danni Chen, Xidong Gu, Chenggong Li, Minya Yao, Jie Pan, Junchi Cheng, Donghai Jiang, Liang Xu, Chang Bao, Peifen Fu, Weimin Fan
Chemoresistance often leads to the failure of breast cancer treatment. MicroRNAs (miRNAs) play an important role in the progression and chemoresistance of cancer. However, because of the complexity of the mechanisms of chemoresistance and the specificity of miRNA regulation in different cell types, the function of miR-20a in breast cancer chemoresistance is still unclear. Here, by using miRNA microarray and high-content screening techniques, we found that miR-20a/b were significantly downregulated in breast cancer tissues compared with normal breast tissues, and low miR-20a/b expression was correlated with poor survival in breast cancer patients...
November 10, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29123601/education-in-cardiopulmonary-resuscitation-in-russia-a-systematic-review-of-the-available-evidence
#19
REVIEW
Alexei Birkun, Maksim Glotov
BACKGROUND: To summarise and appraise cumulative published scientific evidence relevant to cardiopulmonary resuscitation (CPR) education in Russia. DATA RESOURCES: We searched Medline, Scopus, Science Direct and Russian Science Citation Index databases from December 1991 to December 2016 to identify studies pertaining to the field of CPR education that were carried out by Russian researchers and/or investigated the topic of interest for Russia/Russian population...
2017: World Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29119250/microscopy-based-high-throughput-assays-enable-multi-parametric-analysis-to-assess-adverse-effects-of-nanomaterials-in-various-cell-lines
#20
Iris Hansjosten, Juliane Rapp, Luisa Reiner, Ruben Vatter, Susanne Fritsch-Decker, Ravindra Peravali, Taina Palosaari, Elisabeth Joossens, Kirsten Gerloff, Peter Macko, Maurice Whelan, Douglas Gilliland, Isaac Ojea-Jimenez, Marco P Monopoli, Louise Rocks, David Garry, Kenneth Dawson, Peter J F Röttgermann, Alexandra Murschhauser, Joachim O Rädler, Selina V Y Tang, Pete Gooden, Marie-France A Belinga-Desaunay, Abdullah O Khan, Sophie Briffa, Emily Guggenheim, Anastasios Papadiamantis, Iseult Lynch, Eugenia Valsami-Jones, Silvia Diabaté, Carsten Weiss
Manufactured nanomaterials (MNMs) selected from a library of over 120 different MNMs with varied compositions, sizes, and surface coatings were tested by four different laboratories for toxicity by high-throughput/-content (HT/C) techniques. The selected particles comprise 14 MNMs composed of CeO2, Ag, TiO2, ZnO and SiO2 with different coatings and surface characteristics at varying concentrations. The MNMs were tested in different mammalian cell lines at concentrations between 0.5 and 250 µg/mL to link physical-chemical properties to multiple adverse effects...
November 8, 2017: Archives of Toxicology
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