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https://www.readbyqxmd.com/read/28922619/identification-of-angiogenesis-inhibitors-using-a-co-culture-cell-model-in-a-high-content-and-high-throughput-screening-platform
#1
Shuaizhang Li, Chia-Wen Hsu, Srilatha Sakamuru, Chaozhong Zou, Ruili Huang, Menghang Xia
Angiogenesis is an important hallmark of cancer, contributing to tumor formation and metastasis. In vitro angiogenesis models for analyzing tube formation serve as useful tools to study these processes. However, current in vitro co-culture models using primary cells have limitations in usefulness and consistency. Therefore, in the present study, an in vitro co-culture assay system was optimized in a 1536-well format for high-throughput screening using human telomerase reverse transcriptase (hTERT)-immortalized mesenchymal stem cells and aortic endothelial cells...
September 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28920118/hybrid-magnetic-iron-oxide-nanoparticles-with-tunable-field-directed-self-assembly
#2
Vikash Malik, Antara Pal, Olivier Pravaz, Jérôme J Crassous, Simon Granville, Bernard Grobety, Ann M Hirt, Hervé Dietsch, Peter Schurtenberger
We describe the synthesis of hybrid magnetic ellipsoidal nanoparticles that consist of a mixture of two different iron oxide phases, hematite (α-Fe2O3) and maghemite (γ-Fe2O3), and characterize their magnetic field-driven self-assembly. We demonstrate that the relative amount of the two phases can be adjusted in a continuous way by varying the reaction time during the synthesis, leading to strongly varying magnetic properties of the particles. Not only does the saturation magnetization increase dramatically as the composition of the spindles changes from hematite to maghemite, but also the direction of the induced magnetic moment changes from being parallel to the short axis of the spindle to being perpendicular to it...
September 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/28918139/comparison-of-milk-protein-composition-and-rennet-coagulation-properties-in-native-swedish-dairy-cow-breeds-and-high-yielding-swedish-red-cows
#3
Nina A Poulsen, Maria Glantz, Anette K Rosengaard, Marie Paulsson, Lotte B Larsen
Recent studies have reported a very high frequency of noncoagulating milk in Swedish Red cows. The underlying factors are not fully understood. In this study, we explored rennet-induced coagulation properties and relative protein profiles in milk from native Swedish Mountain and Swedish Red Polled cows and compared them with a subset of noncoagulating (NC) and well-coagulating (WC) milk samples from modern Swedish Red cows. The native breeds displayed a very low prevalence of NC milk and superior milk coagulation properties compared with Swedish Red cows...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28916948/viewer-discretion-advised-is-youtube-a-friend-or-foe-in-surgical-education
#4
H Alejandro Rodriguez, Monica T Young, Hope T Jackson, Brant K Oelschlager, Andrew S Wright
BACKGROUND: In the current era, trainees frequently use unvetted online resources for their own education, including viewing surgical videos on YouTube. While operative videos are an important resource in surgical education, YouTube content is not selected or organized by quality but instead is ranked by popularity and other factors. This creates a potential for videos that feature poor technique or critical safety violations to become the most viewed for a given procedure. METHODS: A YouTube search for "Laparoscopic cholecystectomy" was performed...
September 15, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28914485/match-of-psychosocial-risk-and-psychosocial-care-in-families-of-a-child-with-cancer
#5
S M Sint Nicolaas, S A Schepers, E M M van den Bergh, Y de Boer, I Streng, E M van Dijk-Lokkart, M A Grootenhuis, C M Verhaak
OBJECTIVE: The Psychosocial Assessment Tool (PAT) was developed to screen for psychosocial risk, aimed to be supportive in directing psychosocial care to families of a child with cancer. This study aimed to determine (i) the match between PAT risk score and provided psychosocial care with healthcare professionals blind to outcome of PAT assessment, and (ii) the match between PAT risk score and team risk estimation. METHODS: Eighty-three families of children with cancer from four pediatric oncology centers in the Netherlands participated (59% response rate)...
September 15, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28911653/assessment-of-rosehips-based-on-the-content-of-their-biologically-active-compounds
#6
Apurva Bhave, Vera Schulzova, Hana Chmelarova, Libor Mrnka, Jana Hajslova
In this study, an in-depth analysis of the unique set of rosehip samples from 71 Rosa genotypes was conducted with the aim to identify the most suitable ones for applications in the food and pharmaceutical industries based on the content of biologically active compounds. In the first part of our experiments, the antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl assay and the genotypes with the highest values were selected for the follow-up analysis. In the second part of experiments, the major classes of biologically active compounds in rosehips such as carotenoids, tocopherols, flavonoids, and triterpenoic acids were further quantified using liquid chromatography-based techniques...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28903629/a-multidisciplinary-approach-for-developing-an-assessment-tool-for-touch-screen-devices
#7
Alexandra Danial-Saad, Lorenzo Chiari
AIM: The aim of this study was to describe the processes of reaching consensus regarding the assessment of the user's skills required to operate various touch screen devices. A five-step procedure was used to collect and validate the required skills by a multidisciplinary team of 52 experts. Content validity was calculated to determine the agreement levels between the experts. A comparison was made between the discipline groups in order to test correlation between each group and their choice of specific clusters of tasks...
September 14, 2017: Disability and Rehabilitation. Assistive Technology
https://www.readbyqxmd.com/read/28903093/chlorination-chloramination-and-ozonation-of-carbamazepine-enhance-cytotoxicity-and-genotoxicity-multi-endpoint-evaluation-and-identification-of-its-genotoxic-transformation-products
#8
Yingnan Han, Mei Ma, Na Li, Rui Hou, Chao Huang, Yoshimitsu Oda, Zijian Wang
Investigations have focused on the removal and transformation of pharmaceuticals during drinking water and wastewater treatment. In the present study, we investigated for the first time the changes of the cytotoxicity and genotoxicity based on different modes of action (MoAs) during chlorination, chloramination and ozonation processes of the anti-epileptic drug carbamazepine (CBZ). The results illustrated that ozonation enhanced the cytotoxicity and the chromosome damage effects on CHO-K1 cells detected by cytokinesis-block micronucleus (CBMN) assay based on high-content screening technique, though ozonation showed the highest removal efficiency for CBZ...
September 1, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28900089/-high-content-sirna-screen-of-the-kinome-identifies-kinases-involved-in-git2-induced-mesenchymal-epithelial-transition
#9
M G Cao, J Xu, Q F Yang, Z P Guo, K B Zhang, X-B Li, S Q Wu, W Zhou
Epithelial-mesenchymal transition (EMT) and its reverse process mesenchymal-epithelial transition (MET) programs are involced in the metastatic process. More and more evidence confirms that EMT is vital for the initiation and dissemination of cancer cells whereas MET is critical for successful metastatic colonization of a secondary organ. The regulating mechanism of EMT mediated cancer progression and metastasis has been deeply investigated. However, what processes are dependent on MET in metastatic cascades remains unclear...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28899711/colorectal-cancer-screening-associations-between-information-provision-attitudes-and-intended-participation
#10
Stephanie D Brandhof, Angela Fagerlin, Sarah Hawley, Esther Toes-Zoutendijk, Lyndal Trevena, Kirsten McCaffery, Ida J Korfage
OBJECTIVE: Properly informing target audiences is crucial in population-based screening programs. We aimed to evaluate the impact of information about CRC screening on attitudes and intended participation in a screening-naive population. METHODS: 614 persons (aged 55-75 years) received a survey. Information on CRC and screening was provided piece by piece, and per piece its impact on attitudes and intended participation was assessed. All persons received the same information content, but the sequence of information differed per condition: information on the high mortality rate of colorectal cancer was presented in the first or the second piece...
September 9, 2017: Patient Education and Counseling
https://www.readbyqxmd.com/read/28899388/a-review-of-grey-and-academic-literature-of-evaluation-guidance-relevant-to-public-health-interventions
#11
Sarah Denford, Charles Abraham, Margaret Callaghan, Peter Aighton, Frank De Vocht, Steven Arris
BACKGROUND: Public Health evaluation is essential to understanding what does and does not work, and robust demonstration of effectiveness may be crucial to securing future funding. Despite this, programs are often implemented with poor, incomplete or no evaluation. Public health practitioners are frequently required to provide evidence for the effectiveness of their services; thus, there is a growing need for evaluation guidance on how to evaluate public health programs. The aim of this study is to identify accessible high-quality, evaluation guidance, available to researchers and practitioners and to catalogue, summarise and categorise the content of a subset of accessible, quality guides to evaluation...
September 12, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28895613/-the-g-score-a-short-questionnaire-for-the-measurement-of-subjective-physical-health
#12
Juliana Schneider, Markus Zenger, Elmar Brähler, Yve Stöbel-Richter, Hendrik Berth
Aim The measurement of subjective physical health is important in clinical settings as well as for research purposes. In the present paper, the psychometric properties of the G-Score, a 4 item screening questionnaire for the self-assessment of one's physical health, is explored. Methods The Objectivity, validity and reliability were estimated. Moreover, an item analysis and a suitable cut-off-value for the differentiation between healthy and presumably ill subjects were conducted. Data from the Saxony Longitudinal Study ("Sächsische Längsschnittstudie") 1998-2013 were analysed (N=324-417 healthy individuals)...
September 11, 2017: Psychotherapie, Psychosomatik, Medizinische Psychologie
https://www.readbyqxmd.com/read/28895594/determination-of-spectral-markers-of-cytotoxicity-and-genotoxicity-using-in-vitro-raman-microspectroscopy-cellular-responses-to-polyamidoamine-dendrimer-exposure
#13
Esen Efeoglu, Alan Casey, Hugh J Byrne
Although consumer exposure to nanomaterials is ever increasing, with potential increased applications in areas such as drug and/or gene delivery, contrast agents and diagnosis, the determination of the cyto- and geno-toxic effects of nanomaterials on human health and the environment still remains challenging. Although many techniques have been established and adapted to determine the cytotoxicity and genotoxicity of nano-sized materials, these techniques remain limited by the number of assays required, total cost, and use of labels and they struggle to explain the underlying interaction mechanisms...
September 12, 2017: Analyst
https://www.readbyqxmd.com/read/28895365/-expression-of-leafy-cotyledon-2-from-arabidopsis-increased-the-content-of-lipid-in-chlorella-sorokiniana
#14
Changbin Liu, Shaoming Tong, Wenlei Zhang, Hesheng Hou
The low lipid content is one of the major bottlenecks to realize the industrialization of the algae biodiesel. Improvement of lipid content through global regulation to get high-yield generating algae is a good strategy. Leafy Cotyledon 2 (LEC2) is an important transcription factor for seed maturation and oil accumulation in Arabidopsis. However, there are few reports regarding adoption of LEC2 for lipid accumulation until now. In this study, LEC2 from Arabidopsis was cloned into the plant expression vector pCIMBIA1300 and transformed into C...
June 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28892710/instability-mechanisms-and-early-warning-indicators-for-mesophilic-anaerobic-digestion-of-vegetable-waste
#15
Dong Li, Lin Chen, Xiaofeng Liu, Zili Mei, Haiwei Ren, Qin Cao, Zhiying Yan
In order to elucidate the instability mechanism, screen early warning indicators, and propose control measures, the mesophilic digestion of vegetable waste (VW) was carried out at organic loading rates (OLR) of 0.5, 1.0, and 1.5g volatile solid (VS)/(Ld). The process parameters, including biogas components, volatile fatty acids (VFA), ammonia, pH, total alkalinity (TA), bicarbonate alkalinity (BA), and intermediate alkalinity (IA), were monitored every day. Digestion was inhibited at OLR of 1.5gVS/(Ld). The primary causes of instability are a high sugar and negligible ammonia content, in addition to the feed without effluent recirculation, which led to BA loss...
July 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28890768/isochrysis-maritima-billard-and-gayral-isolated-from-penang-national-park-coastal-waters-as-a-potential-microalgae-for-aquaculture
#16
Mohammad Basri Eshak, Wan Maznah Wan Omar
The importance of polyunsaturated fatty acid (PUFA) in microalgae was widely reported. In this study, six isolated microalgae from Teluk Aling, Penang National Park were screened for PUFA contents. Isochrysis maritima showed the best polyunsaturated fatty acids essential for aquaculture species compared to other microalgal species tested. This species is a good choice as aquaculture feed due to its small size (3-7 μm), which is appropriate size for ingestion. The maximum specific growth rate of this species was also high (0...
July 2017: Tropical Life Sciences Research
https://www.readbyqxmd.com/read/28890364/sequential-screening-by-clonepix-fl-and-intracellular-staining-facilitate-isolation-of-high-producer-cell-lines-for-monoclonal-antibody-manufacturing
#17
Gargi Roy, Guillermo Miro-Quesada, Li Zhuang, Tom Martin, Jie Zhu, Herren Wu, Marcello Marelli, Michael A Bowen
The generation of stable cell lines that express therapeutic monoclonal antibodies (mAbs) for manufacturing purposes is a time and labor intensive process. Since cell line development is on the critical path to clinical testing, timely generation of highly productive manufacturing cell lines is essential to remain competitive in the biopharmaceutical industry. To aid this essential function we have developed a methodology for the selection of antibody expressing cells using fluorescence based ClonePix FL colony isolation and flow cytometry analysis following intracellular staining for immunoglobulin G (IgG)...
September 7, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28884359/development-of-a-shear-stress-free-microfluidic-gradient-generator-capable-of-quantitatively-analyzing-single-cell-morphology
#18
David Barata, Giulia Spennati, Cristina Correia, Nelson Ribeiro, Björn Harink, Clemens van Blitterswijk, Pamela Habibovic, Sabine van Rijt
Microfluidics, the science of engineering fluid streams at the micrometer scale, offers unique tools for creating and controlling gradients of soluble compounds. Gradient generation can be used to recreate complex physiological microenvironments, but is also useful for screening purposes. For example, in a single experiment, adherent cells can be exposed to a range of concentrations of the compound of interest, enabling high-content analysis of cell behaviour and enhancing throughput. In this study, we present the development of a microfluidic screening platform where, by means of diffusion, gradients of soluble compounds can be generated and sustained...
September 7, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28884260/barriers-and-facilitators-of-responding-to-problem-gambling-perspectives-from-australian-mental-health-services
#19
S N Rodda, V Manning, N A Dowling, S J Lee, D I Lubman
Despite high rates of comorbidity between problem gambling and mental health disorders, few studies have examined barriers or facilitators to the implementation of screening for problem gambling in mental health services. This exploratory qualitative study identified key themes associated with screening in mental health services. Semi-structured interviews were undertaken with 30 clinicians and managers from 11 mental health services in Victoria, Australia. Major themes and subthemes were identified using qualitative content analysis...
September 7, 2017: Journal of Gambling Studies
https://www.readbyqxmd.com/read/28884067/optimization-of-thermostable-organic-solvent-tolerant-lipase-production-by-thermotolerant-rhizopus-sp-using-solid-state-fermentation-of-palm-kernel-cake
#20
Fatimah Azizah Riyadi, Md Zahangir Alam, Md Noor Salleh, Hamzah Mohd Salleh
This study enhanced the production of thermostable organic solvent-tolerant (TS-OST) lipase by locally isolated thermotolerant Rhizopus sp. strain using solid-state fermentation (SSF) of palm kernel cake (PKC). The optimum conditions were achieved using a series of statistical approaches. The cultivation parameters, which include fermentation time, moisture content, temperature, pH, inoculum size, various carbon and nitrogen sources, as well as other supplements, were initially screened by the definitive screening design, and one-factor-at-a-time using PKC as the basal medium...
October 2017: 3 Biotech
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