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Lipolaser

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https://www.readbyqxmd.com/read/27196312/efficient-chemoenzymatic-synthesis-of-4-nitrophenyl-%C3%AE-d-apiofuranoside-and-its-use-in-screening-of-%C3%AE-d-apiofuranosidases
#1
Peter Kis, Elena Potocká, Vladimír Mastihuba, Mária Mastihubová
4-Nitrophenyl β-d-apiofuranoside as a chromogenic probe for detection of β-d-apiofuranosidase activity was prepared in 61% yield from 2,3-isopropylidene-α,β-d-apiofuranose through a sequence of five reactions. The synthesis involves one regioselective enzymatic step-benzoylation of primary hydroxyl of 2,3-isopropylidene-α,β-d-apiofuranose catalysed by Lipolase 100T and stereoselective β-d-apiofuranosylation of p-nitrophenol using BF3⋅OEt2/Et3N. The product was used for screening of β-d-apiofuranosidase activity in 61 samples of crude commercial enzymes and plant materials...
July 22, 2016: Carbohydrate Research
https://www.readbyqxmd.com/read/26471666/physical-functional-and-structural-characterization-of-the-cell-wall-fractions-from-baker-s-yeast-saccharomyces-cerevisiae
#2
Chema Borchani, Fabienne Fonteyn, Guilhem Jamin, Michel Paquot, Philippe Thonart, Christophe Blecker
The yeast cell wall of Saccharomyces cerevisiae is an important source of β-d-glucan, a glucose homopolymer with many functional, nutritional and human health benefits. In the present study, the yeast cell wall fractionation process involving enzymatic treatments (savinase and lipolase enzymes) affected most of the physical and functional characteristics of extracted fractions. Thus, the fractionation process showed that β-d-glucan fraction F4 had significantly higher swelling power and fat binding capacity compared to other fractions (F1, F2 and F3)...
March 1, 2016: Food Chemistry
https://www.readbyqxmd.com/read/26026940/structural-insights-into-methanol-stable-variants-of-lipase-t6-from-geobacillus-stearothermophilus
#3
Adi Dror, Margarita Kanteev, Irit Kagan, Shalev Gihaz, Anat Shahar, Ayelet Fishman
Enzymatic production of biodiesel by transesterification of triglycerides and alcohol, catalyzed by lipases, offers an environmentally friendly and efficient alternative to the chemically catalyzed process while using low-grade feedstocks. Methanol is utilized frequently as the alcohol in the reaction due to its reactivity and low cost. However, one of the major drawbacks of the enzymatic system is the presence of high methanol concentrations which leads to methanol-induced unfolding and inactivation of the biocatalyst...
November 2015: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/25116359/key-residues-responsible-for-enhancement-of-catalytic-efficiency-of-thermomyces-lanuginosus-lipase-lip-revealed-by-complementary-protein-engineering-strategy
#4
Xiao-Jun Li, Ren-Chao Zheng, Hong-Ye Ma, Jian-Feng Huang, Yu-Guo Zheng
The variant Lip-T (S88T/A99N/V116D) of lipase Lip from Thermomyces lanuginosus has been proved to be a potential biocatalyst for kinetic resolution of 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester (CNDE) to produce valuable chiral intermediate of Pregabalin. In this study, random, site-directed and site-saturation mutagenesis were performed to further enhance the activity of Lip-T, and the key residues responsible for catalytic efficiency were revealed. A mutant S63L/D232A with improved activity toward CNDE was obtained after screening of approximately 2500 clones from random-mutant libraries...
October 20, 2014: Journal of Biotechnology
https://www.readbyqxmd.com/read/24979527/covalent-attachment-of-lipases-on-glyoxyl-agarose-beads-application-in-fruit-flavor-and-biodiesel-synthesis
#5
Adriano A Mendes, Heizir F de Castro, Raquel L C Giordano
The aim of this work was to prepare biocatalysts to catalyze the synthesis of butyl butyrate by esterification reaction, and the synthesis of biodiesel by transesterification of palm and babassu oils with ethanol. Lipase preparations Lipolase® (TLL1) and Lipex® 100 L (TLL2) from Thermomyces lanuginosus and Lipase AK from Pseudomonas fluorescens (PFL) were immobilized on glyoxyl-agarose beads prepared by activation with glycidol (Gly) and epichlorohydrin (Epi). The influence of immobilization time, lipase source and activating agents on the catalytic activity of the biocatalysts were evaluated in both aqueous and organic media...
September 2014: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/23953194/donor-specificity-and-regioselectivity-in-lipolase-mediated-acylations-of-methyl-%C3%AE-d-glucopyranoside-by-vinyl-esters-of-phenolic-acids-and-their-analogues
#6
Mária Mastihubová, Vladimír Mastihuba
Methyl α-D-glucopyranoside as a model acceptor was acylated by several phenolic and non-phenolic vinyl esters using immobilised Lipolase. Donor specificity and regioselectivity of reaction were investigated. Conversion and rate of acylation by structurally varied donors indicates that the synthetic reactivity of Lipolase corresponds to the hydrolytic activity of feruloyl esterase type A. Lipolase exhibited remarkable regioselectivity for primary position of methyl α-D-glucopyranoside. The acylation occurred exclusively at 6-O primary position when vinyl esters of phenolic acids (hydroxybenzoates, hydroxyphenylalkanoates and hydroxycinnamates) served as acyl donors (5-77%)...
October 1, 2013: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/23923547/methods-for-inhibition-of-residual-lipase-activity-in-colorimetric-assay-a-comparative-study
#7
COMPARATIVE STUDY
Shamsher Singh Kanwar, Rajeev Kumar Kaushal, Arshad Jawed, Reena Gupta, Swapandeep Singh Chimni
A comparative study of various treatments for inhibition of the residual activity of a lipase (obtained from Bacillus coagulans MTCC-6375) in a colorimetric assay using p-nitrophenyl palmitate (pNPP) was made. Direct chilling of contents of reaction mixture or addition of chilled mixture of ethanol : acetone (1:1) decreased the residual lipase activity by 94.0 and 95.0% respectively, as compared to lipase incubated at 45 degrees C for 20 min (control). Amongst various ionic and non-ionic detergents, Triton X-100 (0...
August 2005: Indian Journal of Biochemistry & Biophysics
https://www.readbyqxmd.com/read/22676042/lipase-applications-in-oil-hydrolysis-with-a-case-study-on-castor-oil-a-review
#8
REVIEW
Debajyoti Goswami, Jayanta Kumar Basu, Sirshendu De
Lipase (triacylglycerol acylhydrolase) is a unique enzyme which can catalyze various types of reactions such as hydrolysis, esterification, alcoholysis etc. In particular, hydrolysis of vegetable oil with lipase as a catalyst is widely studied. Free lipase, lipase immobilized on suitable support, lipase encapsulated in a reverse micelle and lipase immobilized on a suitable membrane to be used in membrane reactor are the most common ways of employing lipase in oil hydrolysis. Castor oil is a unique vegetable oil as it contains high amounts (90%) of a hydroxy monounsaturated fatty acid named ricinoleic acid...
March 2013: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/22178764/gene-cloning-and-molecular-characterization-of-the-talaromyces-thermophilus-lipase-catalyzed-efficient-hydrolysis-and-synthesis-of-esters
#9
Ines Belhaj-Ben Romdhane, Fakher Frikha, Inès Maalej-Achouri, Ali Gargouri, Hafedh Belghith
A genomic bank from Talaromyces thermophilus fungus was constructed and screened using a previously isolated fragment lipase gene as probe. From several clones isolated, the nucleotide sequence of the lipase gene (TTL gene) was completed and sequenced. The TTL coding gene consists of an open reading frame (ORF) of 1083bp encoding a protein of 269 Aa with an estimated molecular mass of 30kDa. The TTL belongs to the same gene family as Thermomyces lanuginosus lipase (TLL, Lipolase®), a well known lipase with multiple applications...
February 15, 2012: Gene
https://www.readbyqxmd.com/read/20108090/nd-yag-laser-assisted-liposuction-for-an-hiv-patient
#10
M G Onesti, P Fioramonti, S Carella, G Spinelli, N Scuderi
Active antiretroviral therapy has reduced the mortality of patients with acquired immune deficiency syndrome (AIDS) and increased both the quality of life and the longevity of patients infected with human immunodeficiency virus (HIV). The long-term effects of HIV infection are increasingly observed, particularly changes in fat distribution or "lipodystrophy." Strategies to prevent, mitigate, or reverse HIV-associated lipodystrophy have been difficult to develop. The medical management of fat redistribution usually is ineffective, and surgical approaches to HIV-associated lipodystrophy have already been described, but major drawbacks include recurrence of the fat accumulation...
August 2010: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/18623477/comparison-of-hydrolysis-and-esterification-behavior-of-humicola-lanuginosa-and-rhizomucor-miehei-lipases-in-aot-stabilized-water-in-oil-microemulsions-ii-effect-of-temperature-on-reaction-kinetics-and-general-considerations-of-stability-and-productivity
#11
G E Crooks, G D Rees, B H Robinson, M Svensson, G R Stephenson
Humicola lanuginosa lipase (HIL) and Rhizomucor miehei lipase (RrnL), isolated from commercial preparations of Lipolase and Lipozyme, respectively, were solubilized in AOT-stabilized water-in-oil (w/o) microemulsions in n-heptane and aspects of their hydrolysis and condensation activity examined. The temperature dependence of HIL hydrolysis activity in unbuffered R = 10 microemulsions matched very closely that for tributyrin hydrolysis by Lipolase in an aqueous emulsion assay. Apparent activation energies were measured as 13 +/- 2 and 15 +/- 2 kJ mol / respectively...
November 5, 1995: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/18623462/comparison-of-hydrolysis-and-esterification-behavior-of-humicola-lanuginosa-and-rhizomucor-miehei-lipases-in-aot-stabilized-water-in-oil-microemulsions-i-effect-of-ph-and-water-content-on-reaction-kinetics
#12
G E Crooks, G D Rees, B H Robinson, M Svensson, G R Stephenson
Lipolase and Lipozyme are produced in large quantities (as a result of genetic engineering and overexpression) for the detergents market and provide a cheap source of highly active biocatalysts. Humicola lanuginosa lipase (HIL) and Rhizomucor miehei lipase (RmL) have been isolated in partially purified form from commercial preparations of Lipolase and Lipozyme, respectively. These lipases were solubilized in Aerosol-OT (AOT)-stabilized water-in-oil (w/o) microemulsions in n-heptane. HIL and RmL activity in these microemulsions was assayed by spectrophotometric measurement of the initial rate of p-nitophenyl butyrate hydrolysis, and by chromatographic determination of the initial rate of octyl decanoate synthesis from 1-octanol and decanoic acid...
October 5, 1995: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/17658538/comparison-of-chromatographic-ion-exchange-resins-vi-weak-anion-exchange-resins
#13
COMPARATIVE STUDY
Arne Staby, Randi Holm Jensen, Matthias Bensch, Jürgen Hubbuch, Dorte L Dünweber, Janus Krarup, Jacob Nielsen, Mette Lund, Steffen Kidal, Thomas Budde Hansen, Inge Holm Jensen
A comparative study on weak anion exchangers was performed to investigate the pH dependence, binding strength, particle size distribution, and static and dynamic capacity of the chromatographic resins. The resins tested included: DEAE Sepharose FF, Poros 50 D, Fractogel EMD DEAE (M), MacroPrep DEAE Support, DEAE Ceramic HyperD 20, and Toyopearl DEAE 650 M. Testing was performed with five different model proteins: Anti-FVII mAb (immunoglobulin G), aprotinin, bovine serum albumin (BSA), Lipolase (Novozymes), and myoglobin...
September 14, 2007: Journal of Chromatography. A
https://www.readbyqxmd.com/read/17625272/synthesis-of-isoamyl-laurate-and-isoamyl-stearate-in-supercritical-carbon-dioxide
#14
COMPARATIVE STUDY
Mahesh N Varma, Giridhar Madras
The synthesis of isoamyl laurate and isoamyl stearate was studied in supercritical carbon dioxide with three lipases, Novozym 435, Lipolase 100T, and Candida rugosa. The maximum conversion of 37% and 53%, respectively for isoamyl laurate and isoamyl stearate was obtained when Novozym 435 was used. The effect of various parameters such as molar ratio of alcohol to acid, presence of water, time and temperature was investigated. An optimum temperature of 40-45 degrees C was observed for all reactions. The kinetics of reactions was fast and equilibrium was achieved in 2-3 h...
April 2007: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/16856169/vcd-spectroscopic-investigation-of-enantiopure-cyclic-beta-lactams-obtained-through-lipolase-catalyzed-enantioselective-ring-opening-reaction
#15
Elemér Vass, Miklós Hollósi, Eniko Forró, Ferenc Fülöp
A direct enzymatic method for the preparation of cyclic beta-lactams and beta-amino acids was recently developed, involving the Lipolase-catalyzed enantioselective hydrolysis of racemic beta-lactams in an organic solvent. Vibrational circular dichroism (VCD) spectroscopy combined with quantum chemical calculations at ab initio (DFT) level of theory has now been applied to determine the absolute configuration and conformation of a series of cyclic beta-lactams (1-10). The absolute configuration of 8 was derived from X-ray crystallography...
September 2006: Chirality
https://www.readbyqxmd.com/read/16382480/an-efficient-enzymatic-synthesis-of-benzocispentacin-and-its-new-six-and-seven-membered-homologues
#16
Eniko Forró, Ferenc Fülöp
A very efficient enzymatic method was developed for the synthesis of new enantiomeric benzocispentacin and its six- and seven-membered homologues through the Lipolase (lipase B from Candida antarctica) catalyzed enantioselective (E > 200) ring opening of 3,4-benzo-6-azabicyclo[3.2.0]heptan-7-one, 4,5-benzo-7-azabicyclo[4.2.0]octan-8-one, and 5,6-benzo-8-azabicyclo[5.2.0]nonan-9-one with H2O in iPr2O at 60 degrees C. The (1R,2R)-beta-amino acids (ee > or = 96%, yields > or = 40%) and (1S,6S)-, (1S,7S)-, and (1S,8S)-beta-lactams (ee > 99%, yields > or = 44%) produced could be easily separated...
March 8, 2006: Chemistry: a European Journal
https://www.readbyqxmd.com/read/15711795/single-step-purification-of-lipase-from-burkholderia-multivorans-using-polypropylene-matrix
#17
Namita Gupta, Pooja Rathi, Rajni Singh, Vineet Kumar Goswami, Rani Gupta
Lipase from Burkholderia multivorans was purified with high yields directly from fermentation broth by a single-step purification protocol involving adsorption and desorption. The crude enzyme (lyophilized powder) from B. multivorans was loaded on Accurel (Membrana, Germany), a polypropylene matrix, using butanol as the solvent in a buffer at pH 9.0 and ambient temperature for a period of 12 h. The enzyme adsorbed onto the matrix with high specific activity (33 units mg(-1) protein). This was followed by desorption of the enzyme from the matrix using Triton X-100 as the eluent...
June 2005: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/14505171/hydrolases-in-supercritical-co2-and-their-use-in-a-high-pressure-membrane-reactor
#18
Z Knez, M Habulin, M Primozic
The thermal stability and activity of enzymes in supercritical carbon dioxide (SC CO(2)) and near-critical propane were studied at a pressure of 300 bar in the temperature range 20-90 degrees C. Proteinase from Carica papaya was incubated in microaqueous SC CO(2) at atmospheric pressure in a nonaqueous system. Lipase stability in an aqueous medium at atmospheric pressure and in SC CO(2) as well as near-critical propane at 100 bar and 40 degrees C was studied. In order to investigate the impact of solvent on lipases, these were chosen from different sources: Pseudomonas fluorescences, Rhizpous javanicus, Rhizopus niveus and porcine pancreas...
March 2003: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/12688721/lipase-catalyzed-enantioselective-ring-opening-of-unactivated-alicyclic-fused-beta-lactams-in-an-organic-solvent
#19
Eniko Forró, Ferenc Fülöp
[reaction: see text] A highly efficient and very simple method was developed for the synthesis of enantiopure beta-amino acids (e.g. cispentacin) and beta-lactams through the enzyme-catalyzed enantioselective ring opening of unactivated alicyclic beta-lactams in organic media. High enantioselectivity (E > 200) was observed when the Lipolase (lipase B from Candida antarctica)-catalyzed reactions were performed with H(2)O (1 equiv) in diisopropyl ether at 60 degrees C. The resolved products, obtained in good chemical yield (36-47%), could be easily separated...
April 17, 2003: Organic Letters
https://www.readbyqxmd.com/read/12530560/lipase-catalyzed-hydrolysis-of-tg-containing-acetylenic-fa
#20
Marcel S F Lie Ken Jie, Xun Fua, Maureen M L Lau, M L Chye
Hydrolysis of symmetrical acetylenic TG of type AAA [viz., glycerol tri-(4-decynoate), glycerol tri-(6-octadecynoate), glycerol tri-(9-octadecynoate), glycerol tri-(10-undecynoate), and glycerol tri-(13-docosynoate)] in the presence of eight microbial lipases was studied. Novozyme 435 (Candida antarctica), an efficient enzyme for esterification, showed a significant resistance in the hydrolysis of glycerol tri-(9-octadecynoate) and glycerol tri-(13-docosynoate). Hydrolysis of acetylenic TG with Lipolase 100T (Humicola lanuginosa) was rapidly accomplished...
October 2002: Lipids
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