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Bombyx mori

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https://www.readbyqxmd.com/read/28646211/increasing-cell-density-globally-enhances-the-biogenesis-of-piwi-interacting-rnas-in-bombyx-mori-germ-cells
#1
Shozo Honda, Phillipe Loher, Keisuke Morichika, Megumi Shigematsu, Takuya Kawamura, Yoriko Kirino, Isidore Rigoutsos, Yohei Kirino
Piwi proteins and their bound Piwi-interacting RNAs (piRNAs) are predominantly expressed in the germline and play crucial roles in germline development by silencing transposons and other targets. Bombyx mori BmN4 cells are culturable germ cells that equip the piRNA pathway. Because of the scarcity of piRNA-expressing culturable cells, BmN4 cells are being utilized for the analyses of piRNA biogenesis. We here report that the piRNA biogenesis in BmN4 cells is regulated by cell density. As cell density increased, the abundance of Piwi proteins and piRNA biogenesis factors was commonly upregulated, resulting in an increased number of perinuclear nuage-like granules where Piwi proteins localize...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28630398/hcf-1-encoded-by-baculovirus-acmnpv-is-required-for-productive-nucleopolyhedrovirus-infection-of-non-permissive-tn368-cells
#2
Ami Tachibana, Rina Hamajima, Moe Tomizaki, Takuya Kondo, Yoshie Nanba, Michihiro Kobayashi, Hayato Yamada, Motoko Ikeda
Baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) replicates in both Spodoptera frugiperda Sf21 and Trichoplusia ni Tn368 cells, whereas AcMNPV defective in hcf-1 (host cell-factor 1) gene productively infects only Sf21 cells, indicating that HCF-1 is indispensable for the AcMNPV productive infection of Tn368 cells. Here, we demonstrated that HCF-1 protein transiently expressed in Tn368 cells promotes the DNA synthesis of Hyphantria cunea MNPV (HycuMNPV), Orygia pseudotsugata MNPV and Bombyx mori NPV, which are normally unable to replicate in Tn368 cells...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28629377/quantitative-phosphoproteome-on-the-silkworm-bombyx-mori-cells-infected-with-baculovirus
#3
Jauharotus Shobahah, Shengjie Xue, Dongbing Hu, Cui Zhao, Ming Wei, Yanping Quan, Wei Yu
BACKGROUND: Bombyx mori has become an important model organism for many fundamental studies. Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen to Bombyx mori, yet also an efficient vector for recombinant protein production. A previous study indicated that acetylation plays many vital roles in several cellular processes of Bombyx mori while global phosphorylation pattern upon BmNPV infection remains elusive. METHOD: Employing tandem mass tag (TMT) labeling and phosphorylation affinity enrichment followed by high-resolution LC-MS/MS analysis and intensive bioinformatics analysis, the quantitative phosphoproteome in Bombyx mori cells infected by BmNPV at 24 hpi with an MOI of 10 was extensively examined...
June 19, 2017: Virology Journal
https://www.readbyqxmd.com/read/28627423/severe-developmental-timing-defects-in-the-prothoracicotropic-hormone-ptth-deficient-silkworm-bombyx-mori
#4
Miwa Uchibori-Asano, Takumi Kayukawa, Hideki Sezutsu, Tetsuro Shinoda, Takaaki Daimon
The insect neuropeptide prothoracicotropic hormone (PTTH) triggers the biosynthesis and release of the molting hormone ecdysone in the prothoracic gland (PG), thereby controlling the timing of molting and metamorphosis. Despite the well-documented physiological role of PTTH and its signaling pathway in the PG, it is not clear whether PTTH is an essential hormone for ecdysone biosynthesis and development. To address this question, we established and characterized a PTTH knockout line in the silkworm, Bombyx mori...
June 13, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28624519/itraq-based-quantitative-proteomics-analysis-of-molecular-mechanisms-associated-with-bombyx-mori-lepidoptera-larval-midgut-response-to-bmnpv-in-susceptible-and-near-isogenic-strains
#5
Haizhong Yu, Xueyang Wang, Jiaping Xu, Yan Ma, Shangzhi Zhang, Dong Yu, Dongqiong Fei, Azharuddin Muhammad
Bombyx mori nucleopolyhedrovirus (BmNPV) has been identified as a major pathogen responsible for severe economic loss. Most silkworm strains are susceptible to BmNPV, with only a few highly resistant strains thus far identified. Here we investigated the molecular basis of silkworm resistance to BmNPV using susceptible (the recurrent parent P50) and resistant (near-isogenic line BC9) strains and a combination of iTRAQ-based quantitative proteomics, reverse-transcription quantitative PCR and Western blotting...
June 14, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28623733/a-novel-immune-related-gene-hdd1-of-silkworm-bombyx-mori-is-involved-in-bacterial-response
#6
Kui Zhang, Guangzhao Pan, Yuzu Zhao, Xiangwei Hao, Chongyang Li, Li Shen, Rui Zhang, Jingjing Su, Hongjuan Cui
Insects have evolved an effective immune system to respond to various challenges. In this study, a novel immune-related gene, called BmHDD1, was first charactered in silkworm, Bombyx mori. BmHDD1 contained an ORF of 837bp and encoding a deduced protein of 278 amino acids. BmHDD1 was specifically expressed in hemocytes, and highly expressed at the molting and metamorphosis stages under normal physiological conditions. Our results suggested that BmHDD1 was mainly generated by hemocytes and secreted into hemolymph...
June 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28621470/the-pou-homeodomain-transcription-factor-poum2-and-broad-complex-isoform-2-transcription-factor-induced-by-20-hydroxyecdysone-collaboratively-regulate-vitellogenin-gene-expression-and-egg-formation-in-the-silkworm-bombyx-mori
#7
Y Lin, H Liu, C Yang, J Gu, G Shen, H Zhang, E Chen, C Han, Y Zhang, Y Xu, J Wu, Q Xia
Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates BmVg expression was not clarified. In this study, cell transfection experiments showed that the BmVg promoter containing the POU homeodomain transcription factor POUM2 (POUM2) and BrC-Z2 cis-response elements (CREs) showed a more significant response to 20E than that harbouring only the BrC-Z2 or POUM2 CRE...
June 16, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28618068/molecular-cloning-and-analysis-of-a-c-type-lectin-from-silkworm-bombyx-mori
#8
Toufeeq Shahzad, Ming-Yue Zhan, Pei-Jin Yang, Xiao-Qiang Yu, Xiang-Jun Rao
C-type lectins (CTLs) play a variety of roles in plants and animals. They are involved in animal development, pathogen recognition, and the activation of immune responses. CTLs carry one or more non-catalytic carbohydrate-recognition domains (CRDs) to bind specific carbohydrates reversibly. Here, we report the molecular cloning and functional analysis of a single-CRD CTL, named C-type lectin-S2 (BmCTL-S2) from the domesticated silkmoth Bombyx mori (Lepidoptera: Bombycidae). The ORF of CTL-S2 is 666 bp, which encodes a putative protein of 221 amino acids...
June 15, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28617839/comparative-transcriptomes-analysis-of-the-wing-disc-between-two-silkworm-strains-with-different-size-of-wings
#9
Jing Zhang, Danso Blessing, Chenyu Wu, Na Liu, Juan Li, Sheng Qin, Muwang Li
Wings of Bombyx mori (B. mori) develop from the primordium, and different B. mori strains have different wing types. In order to identify the key factors influencing B. mori wing development, we chose strains P50 and U11, which are typical for normal wing and minute wing phenotypes, respectively. We dissected the wing disc on the 1st-day of wandering stage (P50D1 and U11D1), 2nd-day of wandering stage (P50D2 and U11D2), and 3rd-day of wandering stage (P50D3 and U11D3). Subsequently, RNA-sequencing (RNA-Seq) was performed on both strains in order to construct their gene expression profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28611386/effects-of-transient-high-temperature-treatment-on-the-intestinal-flora-of-the-silkworm-bombyx-mori
#10
Zhenli Sun, Dhiraj Kumar, Guangli Cao, Liyuan Zhu, Bo Liu, Min Zhu, Zi Liang, Sulan Kuang, Fei Chen, Yongjie Feng, Xiaolong Hu, Renyu Xue, Chengliang Gong
The silkworm Bombyx mori is a poikilotherm and is therefore sensitive to various climatic conditions. The influence of temperature on the intestinal flora and the relationship between the intestinal flora and gene expression in the silkworm remain unknown. In the present study, changes of the intestinal flora at 48, 96 and 144 h following transient high temperature treatment (THTT) of 37 °C for 8 h were investigated. According to principal component analysis, the abundances of Enterococcus and Staphylococcus showed a negative correlation with other dominant genera...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28610907/insulin-like-growth-factor-igf-like-peptide-and-20-hydroxyecdysone-regulate-the-growth-and-development-of-the-male-genital-disk-through-different-mechanisms-in-the-silkmoth-bombyx-mori
#11
Daiki Fujinaga, Yusuke Kohmura, Naoki Okamoto, Hiroshi Kataoka, Akira Mizoguchi
It is well established that ecdysteroids play pivotal roles in the regulation of insect molting and metamorphosis. However, the mechanisms by which ecdysteroids regulate the growth and development of adult organs after pupation are poorly understood. Recently, we have identified insulin-like growth factor (IGF)-like peptides (IGFLPs), which are secreted after pupation under the control of 20-hydroxyecdysone (20E). In the silkmoth, Bombyx mori, massive amounts of Bombyx-IGFLP (BIGFLP) are present in the hemolymph during pupal-adult development, suggesting its importance in the regulation of adult tissue growth...
June 10, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28607081/bioengineered-silkworms-with-butterfly-cytotoxin-modified-silk-glands-produce-sericin-cocoons-with-a-utility-for-a-new-biomaterial
#12
Ryosuke Otsuki, Masafumi Yamamoto, Erika Matsumoto, Shin-Ichi Iwamoto, Hideki Sezutsu, Masumi Suzui, Keiko Takaki, Keiji Wakabayashi, Hajime Mori, Eiji Kotani
Genetically manipulated organisms with dysfunction of specific tissues are crucial for the study of various biological applications and mechanisms. However, the bioengineering of model organisms with tissue-specific dysfunction has not progressed because the challenges of expression of proteins, such as cytotoxins, in living cells of individual organisms need to be overcome first. Here, we report the establishment of a transgenic silkworm (Bombyx mori) with posterior silk glands (PSGs) that was designed to express the cabbage butterfly (Pieris rapae) cytotoxin pierisin-1A (P1A)...
June 12, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28597953/a-clip-domain-serine-protease-involved-in-moulting-in-the-silkworm-bombyx-mori-cloning-characterization-expression-patterns-and-functional-analysis
#13
H-W Liu, L-L Wang, Z Meng, X Tang, Y-S Li, Q-Y Xia, P Zhao
Clip domain serine proteases (CLIPs), characterized by one or more conserved clip domains, are essential components of extracellular signalling cascades in various biological processes, especially in innate immunity and the embryonic development of insects. Additionally, CLIPs may have additional non-immune functions in insect development. In the present study, the clip domain serine protease gene Bombyx mori serine protease 95 (BmSP95), which encodes a 527-residue protein, was cloned from the integument of B...
June 9, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28595904/characterization-of-armitage-and-yb-containing-granules-and-their-relationship-to-nuage-in-ovary-derived-cultured-silkworm-cell
#14
Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe
PIWI-interacting RNAs (piRNAs) are a class of endogenous small non-coding RNAs, which are mostly 24-32 nucleotides in length and interact specifically with PIWI subfamily of argonaute proteins. Despite the significant research progress in germ line piRNA pathway, its role in somatic cell is not well known. In Drosophila ovarian somatic cell, maturation of primary piRNA and its loading onto Piwi occurs at perinuclear Yb body. The Armitage (Armi) and Yb proteins are the major components of Yb body and specially expressed in ovarian somatic cell...
June 5, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28583832/yorkie-ca-overexpression-in-the-posterior-silk-gland-improves-silk-yield-in-bombyx-mori
#15
Panli Zhang, Shumin Liu, Hong-Sheng Song, Guozheng Zhang, Qiangqiang Jia, Sheng Li
The traditional hybrid breeding techniques can no longer meet the increasing demands for silk production by the silkworm, Bombyx mori, and further improvement of the silk yield will depend on modern molecular breeding techniques. Here, we report improved silk yield in transgenic silkworms overexpressing the oncogene Yorkie(CA) specifically in the posterior silk gland (PSG). The Yorkie(CA) cDNA was ligated downstream of the hr3 enhancer and the fibroin L-chain (Fil) promoter, then inserted into a piggyBac vector for transgene...
June 2, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28577927/deletion-of-the-bombyx-mori-odorant-receptor-co-receptor-bmorco-impairs-olfactory-sensitivity-in-silkworms
#16
Qun Liu, Wei Liu, Baosheng Zeng, Guirong Wang, Dejun Hao, Yongping Huang
Olfaction plays an essential role in many important insect behaviors such as feeding and reproduction. To detect olfactory stimuli, an odorant receptor co-receptor (Orco) is required. In this study, we deleted the Orco gene in the Lepidopteran model insect, Bombyx mori, using a binary transgene-based clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas9 system. We initially generated somatic mutations in two targeted sites, from which we obtained homozygous mutants with deletion of a 866 base pair sequence...
May 31, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28569426/structure-and-expression-of-sulfatase-and-sulfatase-modifying-factor-genes-in-the-diamondback-moth-plutella-xylostella-l
#17
Xiao-Li Ma, Wei-Yi He, Wei Chen, Xue-Jiao Xu, Wei-Ping Qi, Ming-Min Zou, Yan-Chun You, Simon W Baxter, Ping Wang, Min-Sheng You
The diamondback moth, Plutella xylostella (L.), uses sulfatases (SULF) to counteract the glucosinolate-myrosinase defensive system that cruciferous plants have evolved to deter insect feeding. Sulfatase activity is regulated by post-translational modification of a cysteine residue by sulfatase modifying factor 1 (SUMF1). We identified 12 SULF genes (PxylSulfs) and two SUMF1 genes (PxylSumf1s) in the P. xylostella genome. Phylogenetic analysis of SULFs and SUMFs from P. xylostella, Bombyx mori, Manduca sexta, Heliconius melpomene, Danaus plexippus, Drosophila melanogaster, Tetranychus urticae and Homo sapiens showed that the SULFs were clustered into five groups, and the SUMFs could be divided into two groups...
June 1, 2017: Insect Science
https://www.readbyqxmd.com/read/28569067/comparative-proteomic-analysis-of-posterior-silk-glands-of-wild-and-domesticated-silkworms-reveals-functional-evolution-during-domestication
#18
Jian-Ying Li, Fang Cai, Xiao-Gang Ye, Jian-She Liang, Jianke Li, Mei-Yu Wu, Dan Zhao, Zhen-Dong Jiang, Zheng-Ying You, Bo-Xiong Zhong
The wild silkworm Bombyx mandarina was domesticated to produce silk in China approximately 5000 years ago. Silk production is greatly improved in the domesticated silkworm B. mori, but the molecular basis of the functional evolution of silk gland remains elusive. We performed shotgun proteomics with label-free quantification analysis and identified 1012 and 822 proteins from the posterior silk glands (PSGs) of wild silkworms on the 3rd and 5th days of the fifth instar, respectively, with 128 of these differentially expressed...
June 1, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28556443/swath-based-quantitative-proteomics-reveals-the-mechanism-of-enhanced-bombyx-mori-nucleopolyhedrovirus-resistance-in-silkworm-reared-on-uv-b-treated-mulberry-leaves
#19
Jin Hu, Wei Zhu, Yaohan Li, Qijie Guan, Haijian Yan, Jiaojiao Yu, Zhirong Fu, Xingmeng Lu, Jingkui Tian
Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most acute infectious diseases in silkworm, which has led to great economic loss in sericulture. Previous study showed that the content of secondary metabolites in mulberry leaves, particularly for moracin N, was increased after UV-B irradiation. In this study, the BmNPV resistance of silkworms reared on UV-B treated and moracin N spreaded mulberry leaves was improved. To uncover the mechanism of enhanced BmNPV resistance, silkworm midguts from UV-B treated mulberry leaves (BUM) and moracin N (BNM) groups were analyzed by SWATH-based proteomic technique...
May 27, 2017: Proteomics
https://www.readbyqxmd.com/read/28549565/transient-leg-deformations-during-eclosion-out-of-a-tight-confinement-a-comparative-study-on-seven-species-of-flies-moths-ants-and-bees
#20
Leonid Frantsevich, Iryna Kozeretska, Yuriy Dubrovsky, Tetyana Markina, Iryna Shumakova, Stanislav Stukalyuk
Legs in dipteran pupae are tightly packed in a zigzag configuration. Changes in the shape or configuration of long podomeres during eclosion have been overlooked because they occur rapidly (in a few minutes) and the legs are hidden inside a tight opaque confinement: the puparium in the Cyclorrhapha, the obtect pupa in mosquitoes. We fixed insects at different times during eclosion and obtained a temporal description of changes in leg shape. At the start of eclosion in Calliphora vicina and Drosophila melanogaster, femora are buckled in between the joints...
June 2, 2017: Arthropod Structure & Development
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