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https://www.readbyqxmd.com/read/29793044/isolation-of-ferritin-and-its-interaction-with-bmnpv-in-the-silkworm-bombyx-mori
#1
Dong-Qiong Fei, Hai-Zhong Yu, Jia-Ping Xu, Shang-Zhi Zhang, Jie Wang, Bing Li, Li-Ang Yang, Pei Hu, Xin Xu, Kang Zhao, Toufeeq Shahzad
Ferritin is a ubiquitous iron storage protein that plays an important role in host defence against pathogen infections. In the present study, native ferritin was isolated from the hemolymph of Bombyx mori using native-polyacrylamide gel electrophoresis (native-PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed that ferritin consisted of two subunits, designated as BmFerHCH and BmFerLCH. Previously integrated previous transcriptome and iTRAQ data showed that the two subunits were down-regulated in resistant silkworm strain BC9 and there was no obvious change in the expression levels of the subunits in susceptible silkworm strain P50 after BmNPV infection...
May 9, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29778904/c-lysozyme-contributes-to-antiviral-immunity-in-bombyx-mori-against-nucleopolyhedrovirus-infection
#2
Ting-Ting Chen, Li-Rong Tan, Nan Hu, Zhan-Qi Dong, Zhi-Gang Hu, Ya-Ming Jiang, Peng Chen, Min-Hui Pan, Cheng Lu
Lysozymes is a ubiquitous immune effector that is widely distributed in both vertebrates and invertebrates. Previous reports have shown that lysozymes significantly inhibit viral infections in vertebrates. However, the antiviral effects of lysozymes in invertebrates remain unclear. Here, we investigated the role of lysozymes in Bombyx mori (B. mori) response to viral infection by overexpressing B. mori C-lysozyme (BmC-LZM) in larvae and cells. We found that BmC-LZM was up-regulated in cells in response to viral infection...
May 17, 2018: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29760316/unique-sex-determination-system-in-the-silkworm-bombyx-mori-current-status-and-beyond
#3
Susumu Katsuma, Takashi Kiuchi, Munetaka Kawamoto, Toshiaki Fujimoto, Ken Sahara
The silkworm Bombyx mori has been used for silk production for over 5,000 years. In addition to its contribution to sericulture, B. mori has played an important role in the field of genetics. Classical genetic studies revealed that a gene(s) with a strong feminizing activity is located on the W chromosome, but this W-linked feminizing gene, called Feminizer (Fem), had not been cloned despite more than 80 years of study. In 2014, we discovered that Fem is a precursor of a single W chromosome-derived PIWI-interacting RNA (piRNA)...
2018: Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
https://www.readbyqxmd.com/read/29753046/identification-of-two-isoforms-of-pop-in-the-domestic-silkworm-bombyx-mori-cloning-characterization-and-expression-analysis
#4
Ping Fu, Wei Sun, Juan Lai, Yi-Hong Shen, Ze Zhang
Two isoforms, Bmpop-a and Bmpop-b, were cloned and characterized, which were found to encode prolyl oligopeptidase (Pop) of the domestic silkworm Bombyx mori. The full lengths of Bmpop-a and Bmpop-b were 2497 and 2508 bp, deducing 707 and 740 amino acids, respectively. Both of them, possessing the typical characteristics of the Pop family of serine proteinase, were detected to be expressed among different tissues and development stages at the transcription and translation levels. Soluble recombinant BmPop-a (rBmPop-a) had oligopeptidase activity toward the substrates, Z-Gly-Pro-pNA, Z-Gly-Pro-AMC and angiotensin I...
May 9, 2018: Gene
https://www.readbyqxmd.com/read/29743532/a-single-amino-acid-substitution-in-the-bombyx-specific-mucin-like-membrane-protein-causes-resistance-to-bombyx-mori-densovirus
#5
Katsuhiko Ito, Kurako Kidokoro, Susumu Katsuma, Hideki Sezutsu, Keiro Uchino, Isao Kobayashi, Toshiki Tamura, Kimiko Yamamoto, Kazuei Mita, Toru Shimada, Keiko Kadono-Okuda
Bombyx mori densovirus type 1 (BmDV) is a pathogen that causes flacherie disease in the silkworm. The absolute nonsusceptibility to BmDV among certain silkworm strains is determined independently by two genes, nsd-1 and Nid-1. However, neither of these genes has been molecularly identified to date. Here, we isolated the nsd-1 gene by positional cloning and characterized the properties of its product, NSD-1. Sequence and biochemical analyses revealed that this gene encodes a Bombyx-specific mucin-like glycoprotein with a single transmembrane domain...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29740149/clathrin-mediated-endocytosis-is-a-candidate-entry-sorting-mechanism-for-bombyx-mori-cypovirus
#6
Fei Chen, Liyuan Zhu, Yiling Zhang, Dhiraj Kumar, Guangli Cao, Xiaolong Hu, Zi Liang, Sulan Kuang, Renyu Xue, Chengliang Gong
Bombyx mori cypovirus (BmCPV), a member of the Reoviridae, specifically infects silkworms and causes extensive economic losses to the sericulture industry. To date, the entry mechanism of BmCPV into cells is unclear. Here we used electron microscopy to study the route of entry of BmCPV into cells, and the results demonstrated that the entry of BmCPV into BmN cells was mediated by endocytosis. Blocking the entry pathway with four endocytosis inhibitors, including dansylcadaverine, chlorpromazine, genistein, and PP2, significantly decreased the infectivity of BmCPV...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29740071/silk-fibroin-micro-particle-scaffolds-with-superior-compression-modulus-and-slow-bioresorption-for-effective-bone-regeneration
#7
Anuya Nisal, Raeesa Sayyad, Prachi Dhavale, Bhakti Khude, Rucha Deshpande, Vidhyashri Mapare, Swati Shukla, Premnath Venugopalan
Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29733628/plasma-ion-implantation-of-silk-biomaterials-enabling-direct-covalent-immobilization-of-bioactive-agents-for-enhanced-cellular-responses
#8
Alexey Kondyurin, Kieran Lau, Fengying Tang, Behnam Akhavan, Wojciech Chrzanowski, Megan S Lord, Jelena Rnjak-Kovacina, Marcela M M Bilek
Silk fibroin isolated from Bombyx mori cocoons is a promising material for a range of biomedical applications, but it has no inherent cell-interactive domains, necessitating functionalization with bioactive molecules. Here we demonstrate significantly enhanced cell interactions with silk fibroin biomaterials in the absence of biofunctionalization following surface modification using plasma immersion ion implantation (PIII). Further, PIII treated silk fibroin biomaterials supported direct covalent immobilization of proteins on the material surface in the absence of chemical cross-linkers...
May 7, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29730425/downregulation-of-aldose-reductase-is-responsible-for-developmental-abnormalities-of-the-silkworm-purple-quail-like-mutant-q-l-p
#9
Pingyang Wang, Simin Bi, Weiyang Wei, Zhiyong Qiu, Dingguo Xia, Xingjia Shen, Qiaoling Zhao
Aldose reductase (AR) is a rate-limiting enzyme in the polyol pathway and is also the key enzyme involved in diabetic complications. The silkworm purple quail-like mutant (q-lp ) exhibits pigmented dots on its epidermis. The q-lp mutant also shows developmental abnormalities and decreased vitality. In this study, fat bodies from the q-lp mutant and the wildtype 932VR strain were subjected to two-dimensional gel electrophoresis (2-DE) analysis, and the Bombyx mori AR (BmAR) protein was found to be significantly downregulated in the q-lp mutant...
May 3, 2018: Gene
https://www.readbyqxmd.com/read/29730398/capa-periviscerokinin-mediated-activation-of-mapk-erk-signaling-through-gq-plc-pkc-dependent-cascade-and-reciprocal-erk-activation-dependent-internalized-kinetics-of-bom-capa-pvk-receptor-2
#10
Zhangfei Shen, Xiaoyuan Yang, Yu Chen, Liangen Shi
Bombyx mori neuropeptide G protein-coupled receptor (BNGR)-A27 is a specific receptor for B. mori capability (CAPA) periviscerokinin (PVK), that is, Bom-CAPA-PVK receptor 2. Upon stimulation of Bom-CAPA-PVK-1 or -PVK-2, Bom-CAPA-PVK receptor 2 significantly increases cAMP-response element-controlled luciferase activity and Ca2+ mobilization in a Gq inhibitor-sensitive manner. However, the underlying mechanism(s) for CAPA/CAPA receptor system mediation of extracellular signal-regulated kinases1/2 (ERK1/2) activation remains to be explained further...
May 3, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29725106/investigation-of-immunogenic-properties-of-hemolin-from-silkworm-bombyx-mori-as-carrier-protein-an-immunoinformatic-approach
#11
Veeranarayanan Surya Aathmanathan, Nattarsingam Jothi, Vijay Kumar Prajapati, Muthukalingan Krishnan
Infectious diseases are the major cause of high mortality among infants and geriatric patients. Vaccines are the only weapon in our arsenal to defend us ourselves against innumerable infectious diseases. Though myriad of vaccines are available, still countless people die due to microbial infections. Subunit vaccine is an effective strategy of vaccine development, combining a highly immunogenic carrier protein with highly antigenic but non-immunogenic antigen (haptens). In this study we have made an attempt to utilize the immunoinformatic tool for carrier protein development...
May 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29718504/proteomic-based-approach-to-the-proteins-involved-in-1-deoxynojirimycin-accumulation-in-silkworm-bombyx-mori-lepidoptera-bombycidae
#12
Hongxia Chen, Yueqin Liu, Wenbing Wang, Opeyemi J Olatunji, Gang Pan, Zhen Ouyang
1-Deoxynojirimycin (DNJ) is the most abundant poly-hydroxylated alkaloid in the latex of mulberry leaves and it protects mulberry from insect predation. However, silkworms can survive the poisoning effect of DNJ and accumulate DNJ by consumption of the mulberry leaves. In order to determine the molecular mechanism of DNJ accumulation in silkworm, comparative proteomic analysis was employed to evaluate protein expression in two groups of silkworm bodies (the third instar silkworm bodies had the maximum content of DNJ throughout life, and the newly hatched silkworm bodies had no DNJ)...
March 1, 2018: Journal of Insect Science
https://www.readbyqxmd.com/read/29717548/expression-and-activation-of-horseradish-peroxidase-protein-a-g-fusion-protein-in-silkworm-larvae-for-diagnostic-purposes
#13
Patmawati Xxxx, Kosuke Minamihata, Tsuneyuki Tatsuke, Jae Man Lee, Takahiro Kusakabe, Noriho Kamiya
Recombinant protein production can create artificial proteins with desired functions by introducing genetic modifications to the target proteins. Horseradish peroxidase (HRP) has been used extensively as a reporter enzyme in biotechnological applications; however, recombinant production of HRP has not been very successful, hampering the utilization of HRP with genetic modifications. A fusion protein comprising an antibody binding protein and HRP will be an ideal bio-probe for high-quality HRP-based diagnostic systems...
March 25, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29709078/titanium-nanoparticles-influence-the-akt-tor-signal-pathway-in-the-silkworm-bombyx-mori-silk-gland
#14
Bin Xue, Fan-Chi Li, Jiang-Hai Tian, Jin-Xin Li, Xiao-Yu Cheng, Jia-Huan Hu, Jing-Sheng Hu, Bing Li
Various nanoparticles, such as silver nanoparticles (AgNPs) and titanium nanoparticles (TiO2 NPs) are increasingly used in industrial processes. Because they are released into the environment, research into their influence on the biosphere is necessary. Among its other effects, dietary TiO2 NPs promotes silk protein synthesis in silkworms, which prompted our hypothesis that TiO2 NPs influence protein kinase B (Akt)/Target of rapamycin (Tor) signaling pathway (Akt/Tor) signaling in their silk glands. The Akt/Tor signaling pathway is a principle connector integrating cellular reactions to growth factors, metabolites, nutrients, protein synthesis, and stress...
April 30, 2018: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/29704373/-applications-of-the-crispr-cas9-system-in-insects
#15
Xiao Ling Tong, Chun Yan Fang, Tin Ting Gai, Jin Shi, Cheng Lu, Fang Yin Dai
The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated) system guides Cas9 to specific genomic locations by a short RNA search string. This technology enables the systematic interrogation of mammalian genome editing, repairing damaged genes, silencing harmful genes and improving quality traits. In recent years, with the introduction of the CRISPR/Cas9 system for easy, fast and efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species, such as Drosophila, Bombyx mori, Aedes aegypti and butterflies et al...
April 20, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/29694780/combination-of-amorphous-silk-fiber-spinning-and-post-spinning-crystallization-for-tough-regenerated-silk-fibers
#16
Kenjiro Yazawa, Ali D Malay, Nao Ifuku, Takaoki Ishii, Hiroyasu Masunaga, Takaaki Hikima, Keiji Numata
An artificial spinning system using regenerated silk fibroin solutions is adopted to produce high-performance silk fibers. In previous studies, alcohol-based agents, such as methanol or ethanol, were used to coagulate silk dope solutions, producing silk fiber with poor mechanical properties compared with those of native silk fibers. The alcohol-based coagulation agents induce rapid -sheet crystallization of the silk molecules, which inhibits subsequent alignment of the -sheet crystals. Here, we induce gradual -sheet formation to afford adequate -sheet alignment similar to that of native silk fiber...
April 25, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29691828/silk-fibroin-based-hydrogels-and-scaffolds-for-osteochondral-repair-and-regeneration
#17
Viviana P Ribeiro, Sandra Pina, J Miguel Oliveira, Rui L Reis
Osteochondral lesions treatment and regeneration demands biomimetic strategies aiming physicochemical and biological properties of both bone and cartilage tissues, with long-term clinical outcomes. Hydrogels and scaffolds appeared as assertive approaches to guide the development and structure of the new osteochondral engineered tissue. Moreover, these structures alone or in combination with cells and bioactive molecules bring the mechanical support after in vitro and in vivo implantation. Moreover, multilayered structures designed with continuous interfaces furnish appropriate features of the cartilage and subchondral regions, namely microstructure, composition, and mechanical properties...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29689939/illustrated-accounts-of-coccinellid-predators-of-maconellicoccus-hirsutus-green-hemiptera-sternorrhyncha-pseudococcidae-on-mulberry-in-india-with-description-of-a-new-species-of-scymnus-kugelann-coleoptera-coccinellidae-from-west-bengal
#18
J Poorani, N Lalitha
The pink hibiscus mealybug, Maconellicoccus hirsutus (Green), is a major pest of mulberry (Morus alba L.), the sole host of the mulberry silkworm, Bombyx mori (L.), which is a source of livelihood to millions of sericulture farmers in India. Several predators, mainly Coccinellidae (Coleoptera), have been reported to feed on M. hirsutus on mulberry. Coccinellid predators of M. hirsutus collected on mulberry from different parts of India are illustrated here with brief diagnostic notes to facilitate their identification...
February 20, 2018: Zootaxa
https://www.readbyqxmd.com/read/29687606/comparative-proteome-analysis-reveals-that-cuticular-proteins-analogous-to-peritrophin-motif-proteins-are-involved-in-the-regeneration-of-chitin-layer-in-the-silk-gland-of-bombyx-mori-at-the-molting-stage
#19
Xiaolu Zhang, Huaipu Chang, Zhaoming Dong, Yan Zhang, Dongchao Zhao, Lin Ye, Qingyou Xia, Ping Zhao
The silk gland of silkworm produces silk proteins during larval development. Many studies have long focused on the silk gland of the fifth instar larvae, but few have investigated this gland at other larval stages. In the present study, the silk gland proteomes of the fourth instar and fourth molt were analyzed using liquid chromatography-tandem mass spectrometry. In total, 2654 proteins were identified from the silk gland. High abundance of ribosomal proteins and RR-motif chitin-binding proteins were identified during day 2 of the fourth instar (IV-2) larval developmental stage, and the expression of cuticular proteins analogous to peritrophin (CPAP)-motif chitin-binding proteins was higher during the fourth molt (IV-M)...
April 24, 2018: Proteomics
https://www.readbyqxmd.com/read/29684748/silk-fibroin-as-a-platform-for-dual-sensing-of-vitamin-b-12-using-photoluminescence-and-electrical-techniques
#20
Sudesna Chakravarty, Bedanta Gogoi, Biman B Mandal, Nandana Bhardwaj, Neelotpal Sen Sarma
The amalgamation of natural origin materials and technologies for label-free and real time detection is pertinent in analytical field. In this study, Bombyx mori silk fibroin protein (BMSF) has been utilized for the dual sensing of vitamin B12 via fluorescence and electrical impedance techniques. The processing of BMSF is done to generate an aqueous solution of BMSF along with the development of micro patterned thin films via soft lithography technique. The BMSF aqueous solution exhibit auto fluorescence property and thereby utilized for label free detection of vitamin B12 with an estimated limit of detection (LOD) of about 0...
March 28, 2018: Biosensors & Bioelectronics
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