Read by QxMD icon Read

Bombyx mori

Pala Rajasekharreddy, Pathipati Usha Rani, Saidulu Mattapally, Sanjay Kumar Banerjee
The present study investigated the therapeutic action of flavonoids loaded silver nanoparticles (FLV-Ag NPs) on a silkworm, Bombyx mori L., larvae infected by the S. aureus, where an in vivo model system was used. FLV-Ag NPs were synthesized using a Ricinus communis L. leaf extracted flavonoid mixture in various concentrations. The reduction reaction was performed under a simple sunlight exposure condition. In the reduction process, quercetin and kaempferol loadings were also found. They were confirmed by UV-visible, TEM, XRD, XPS, DLS and FTIR spectroscopic techniques...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Kun Yu, Fei Lu, Qing Li, Honglei Chen, Bitao Lu, Jiawei Liu, Zhiquan Li, Fangying Dai, Dayang Wu, Guangqian Lan
Preventing wound infection and retaining an appropriate level of moisture around wounds represent the most critical issues in wound treatment. Towards these ends, special focus has been placed on Bombyx mori cocoons because the protective function of the silkworm cocoon resembles the manner in which the skin protects the human body. We have designed a facile technique to develop a novel silkworm cocoon-based wound film (SCWF) wound dressing utilizing a CaCl2-ethanol-H2O solution. To improve the anti-bacterial performance of SCWF, we have incorporated the ability of silk sericin to act as a reducing agent for the conversion of Ag(+) to Ag, yielding nanoparticles (AgNPs) linked together by peptide bonds of silkworm cocoon wound film (SCWF-AgNPs)...
May 18, 2017: Scientific Reports
Tomohiro Inui, Takaaki Daimon
It is widely accepted that the anti-metamorphic action of insect juvenile hormones (JHs) is required to inhibit larval-pupal metamorphosis. However, recent studies using RNAi or knockout techniques reveal that larval status may be maintained independently of JHs during the early larval stages. To investigate why larvae of very early instars do not have competence to metamorphose and how they acquire this competence through larval development, we revisited the classic experiments of Piepho (ca. 1930s) and performed implantation assays using the integument of very young larvae of the silkworm, Bombyx mori...
May 15, 2017: Journal of Insect Physiology
Wanna Chaisabai, Arunrat Khamhaengpol, Sineenat Siri
Green synthesis of silver nanoparticles (AgNPs) has received many interests as a simple, cost-effective, and environmentally friendly method. This study reported the use of sericins extracted from non-mulberry (Samia cynthia ricini) and mulberry (Bombyx mori) silkworms for green syntheses of AgNPs. Both sericins possessed the reducing activity, which the reducing activity of S. c. ricini sericin was significantly higher than that of B. mori sericin. The formation of AgNPs facilitated by S. c. ricini sericin was greater than B...
May 17, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Dandan Wang, Zhaoming Dong, Yan Zhang, Kaiyu Guo, Pengchao Guo, Ping Zhao, Qingyou Xia
Mulberry leaves have been selected as food source for the silkworm (Bombyx mori) over 5000 years. However, the interaction mechanisms of mulberry-silkworm remain largely unknown. In this study, we try to explore the interaction between mulberry and silkworm at the protein level. Total proteins were extracted from mulberry leaves and silkworm feces on day 5 of the fifth larval instar, and analyzed on shotgun liquid chromatography-tandem mass spectrometry, respectively. In total, 2076 and 210 foliar proteins were identified from mulberry leaves and silkworm feces, respectively...
May 15, 2017: Journal of Proteome Research
Ping Wu, Xiaoxu Jiang, Qi Sang, Enoch Annan, Tao Cheng, Xijie Guo
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the major pathogens that pose a big challenge to the sericulture industry. Growing evidences have shown that microRNAs play key roles in the regulations of host-pathogen interactions in insects. MicroRNAs have been found in silkworms, whether and how they affect the silkworm-BmCPV interactions are still unknown. Here we investigate the effect of miR-274-3p on the BmCPV replication in the BmCPV-infected silkworm larvae. In our study, BmCPV Nonstructural protein 5 (NS5) was identified to be the target of miR-274-3p based on bioinformatics analysis and luciferase reporter assay...
May 10, 2017: Virus Genes
Tetsuo Asakura, Kenta Miyazawa, Yugo Tasei, Shunsuke Kametani, Yasumoto Nakazawa, Akihiro Aoki, Akira Naito
Samia cynthia ricini (S. c. ricini) is one of the wild silkworms. Their silk fibroins have been paid attention as potentially valuable biomedical materials as well as Bombyx mori silk fibroins, but detailed information on the packing arrangement of the fibers is still not currently well understood at a molecular level. In this study, 34 mer model peptides, GGAGGGYGGDGG(A)12GGAGDGYGAG with different (13)C labeled positions have been synthesized as a typical sequence of the primary structure of S. c. ricini silk fibroins made up of tandemly repeated sequences of polyalanine as the crystalline region and glycin-rich sequences as the non-crystalline region...
May 11, 2017: Physical Chemistry Chemical Physics: PCCP
Baosheng Zeng, Yuping Huang, Jun Xu, Takahiro Shiotsuki, Hua Bai, Subba Reddy Palli, Yongping Huang, Anjiang Tan
Forkhead box O (FOXO) functions as the terminal transcription factor of the insulin signaling pathway and regulates multiple physiological processes in many organisms, including lifespan in insects. However, how FOXO interacts with hormone signaling to modulate insect growth and development is largely unknown. Here, using the transgene-based CRISPR/Cas9 system, we generated and characterized mutants of the silkworm Bombyx mori FOXO (BmFOXO) to elucidate its physiological functions during development of this lepidopteran insect...
May 10, 2017: Journal of Biological Chemistry
Xiaolin Zhang, Anmin Jiang, Guisheng Wang, Hao Yu, Banghua Qi, Youyi Xiong, Guoliang Zhou, Meisong Qin, Jinfeng Dou, Jianfei Wang
Helicobacter pylori (H. pylori) shows increasingly enhanced resistance to various antibiotics, and its eradication has become a major problem in medicine. The antimicrobial peptide PGLa-AM1 is a short peptide with 22 amino acids and exhibits strong antibacterial activity. In this study, we investigated whether it has anti-H. pylori activity for the further development of anti-H. pylori drugs to replace existing antibiotics. However, the natural antimicrobial peptide PGLa-AM1 shows a low yield and is difficult to separate, limiting its application...
May 10, 2017: Applied Microbiology and Biotechnology
Xu Wu, Xiaodong Wu, Min Shao, Bin Yang
Here we investigated the structural changes of silk fibroin during Bombyx mori silkworm spinning and reconstitution process. X-ray diffraction, Fourier transform infrared spectroscopy, polarized optical microscopy, and terahertz (THz) spectroscopy were applied to monitor the structural features of silk fibroin from posterior, middle silk glands, to cocoons, and then to reconstituted silk. Results show that from silk gland to cocoon, fibroin experiences a significant transformation in crystal structure from a typical silk I, to a silk I-rich mixed structure, and finally to a typical silk II state, accompanied with a change in secondary structure from α-helix and random coil structures to preferential orientation β-sheets...
May 6, 2017: International Journal of Biological Macromolecules
Weitao Qiu, Jing Wu, Jae-Hoon Choi, Hirofumi Hirai, Hiroshi Nishida, Hirokazu Kawagishi
Two compounds, 3'-deoxyinosine and cordycepin, were isolated from Bombyx mori inoculated with Cordyceps militaris. In the bioassay examining cytotoxicity against cancer cells, both compounds showed toxicity against A549, PANC-1, and MCF-7 cancer cells.
June 2017: Bioscience, Biotechnology, and Biochemistry
Vartika Dhyani, Neetu Singh
Silk biomaterials can be designed to provide architectural framework comparable of connate extracellular matrix in order to boost cell growth and eventual tissue regeneration. Silk (Bombyx mori) fibroins self-assemble into hydrophobic crystalline β sheets, which provide mechanical strength and tunable degradability. The next generation tissue engineering scaffolds aim at providing spatially controlled modulation of cell adhesion and differentiation, which can be achieved by spatially controlled surface functionalization of the scaffolds...
May 9, 2017: Biomedical Materials
Jadi Praveen Kumar, Biman B Mandal
Sericin, a principal constituent of silk, is widely used in various biomedical applications. In addition, conferring protection against free radicals and oxidative damage add more value to its therapeutic potential. However, the antioxidant (AO) properties of silk sericin (SS) remains contingent on extraction procedures. In the present study, we have evaluated the effect of different extraction methods (conventional, autoclaving, urea, alkali and acid-degradation) on AO properties of SS from three Indian silk varieties [Antheraea assamensis (AA), Philosamia ricini (PR) and Bombyx mori (BM)]...
May 2, 2017: Free Radical Biology & Medicine
Hiroaki Mon, Jae Man Lee, Masanao Sato, Takahiro Kusakabe
The kinetochore creates chromosomal attachment sites for microtubules. The kinetochore-microtubule interface plays an important role in ensuring accurate transmission of genetic information to daughter cells. Bombyx mori is known to possess holocentric chromosomes, where spindle microtubules attach along the entire length of the chromosome. Recent evidence suggests that CENP-A and CENP-C, which are essential for centromere structure and function in other species, have lost in holocentric insects, implying that B...
May 1, 2017: Insect Biochemistry and Molecular Biology
André da Costa, Ana Pereira, Andreia Gomes, Jose Rodriguez-Cabello, Vitor Sencadas, Margarida Casal, Raul Machado
Genetically engineered protein polymers functionalized with bioactive domains offer potential as multifunctional versatile materials for biomedical use. The present work describes the fabrication and characterization of antimicrobial fibre mats comprising the antimicrobial elastin-like recombinamer CM4-A200. The CM4-A200 protein polymer derives from the genetic fusion of the ABP-CM4 antimicrobial peptide from <i>Bombyx mori</i> with 200 repetitions of the pentamer VPAVG. This is the first report on non-crosslinked fibre mats fabricated with an antimicrobial elastin-like recombinamer stable in solution...
May 4, 2017: Biomedical Materials
Tatsuya Kato, Natsumi Kako, Kotaro Kikuta, Takatsugu Miyazaki, Sachiko Kondo, Hirokazu Yagi, Koichi Kato, Enoch Y Park
Recombinant proteins produced in insect cells and insects, unlike those produced in mammalian cells, have pauci-mannose-type N-glycans. In this study, we examined complex-type N-glycans on recombinant proteins via coexpression of human β-1,2-N-acetylglucosaminyltransferase II (hGnT II) and human β1,4-galactosyltransferase (hGalT I) in silkworm pupae, by using the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system. The actin A3 promoter from B. mori and the polyhedrin promoter from Autographa californica multiple nucleopolyhedroviruses (AcMNPVs) were used to coexpress hGnT II and hGalT I...
May 3, 2017: Scientific Reports
Xin Wang, Yi Li, Qingsong Liu, Qingyou Xia, Ping Zhao
The silkworm spinneret is an important tissue for silk fibrillogenesis and spinning. All biochemical processes during silk fibrillogenesis are correlated with silk properties. Understanding the role of spinneret in silk fibrillogenesis may help to reveal the mechanism of silk fibrillogenesis as well as improve silk quality for commercial purposes. Thus, we profiled the proteome of silkworm spinneret. A total of 1572 proteins and 232 differential abundance proteins were identified. Silk fibrillogenesis-related proteins, such as cuticle proteins, ion-transporting proteins, muscular proteins, and energy metabolic proteins, were abundant in spinneret...
May 3, 2017: Proteomics
Feng Zhu, Shengyan Xiao, Yonghong Zhang, Yulan Shao, Fenfen Tang, Shiliang Chen, Xingrong Bai
In this report, we describe the cloning and characterization of a member of the immunoglobulin superfamily (IgSF); i.e., Turtle. The cDNA of Turtle was cloned from the silkworm Bombyx mori using the rapid amplification of cDNA ends (RACE) technique. Three isoforms of Bombyx Turtle were obtained, including Bmtutl-464, Bmtutl-519, and Bmtutl-810. The three isoforms had identical 27-amino acid signal peptides and four extracellular immunoglobulin (Ig) domains (IgI-IgIV). Sequence similarity and phylogenic analysis indicated that Bmtutl-810 belongs to the group of insect Turtle isoforms and shares 76...
April 30, 2017: Infection, Genetics and Evolution
Abdelrahman Ibrahim Abushouk, Ammar Ismail, Amr Muhammad Abdo Salem, Ahmed M Afifi, Mohamed M Abdel-Daim
Doxorubicin (DOX) is an anthracycline antibiotic, which is effectively used in the treatment of different malignancies, such as leukemias and lymphomas. Its most serious side effect is dose-dependent cardiotoxicity, which occurs through inducing oxidative stress apoptosis. Due to the myelosuppressive effect of dexrazoxane, a commonly-used drug to alleviate DOX-induced cardiotoxicity, researchers investigated the potential of phytochemicals for prophylaxis and treatment of this condition. Phytochemicals are plant chemicals that have protective or disease preventive properties...
June 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Kantaporn Kheawfu, Surachai Pikulkaew, Hiroshi Hamamoto, Kazuhisa Sekimizu, Siriporn Okonogi
Clove oil is used in fish anesthesia and expected to have a mechanism via glutamic receptor. The present study explores the activities of clove oil and its major compound, eugenol, in comparison with L-glutamic acid on glutamic receptor of silkworm muscle and fish anesthesia. It was found that clove oil and eugenol had similar effects to L-glutamic acid on inhibition of silkworm muscle contraction after treated with D-glutamic acid and kainic acid. Anesthetic activity of the test samples was investigated in goldfish...
April 30, 2017: Drug Discoveries & Therapeutics
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"