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Bombyx mori

Ye Pan, Peng Lü, Qinyun Wang, Feifei Zhu, Chengjun Li, Yuanqing He, Keping Chen
Dietary restriction (DR) refers to a reduction in food intake to induce undernutrition but not malnutrition, which extends the lifespan of multiple species. Although there are invertebrate aging models, such as the Caenorhabditis elegans and Drosophila melanogaster, aging studies in Lepidoptera are few in number and the underlying life-extending molecular mechanisms are not clear. Research on a broader range of animals is necessary to support generalizations on mechanisms of aging and rates of aging. The aim of this study was to further investigate genes and pathways associated with DR in Bombyx mori...
May 2017: Archives of Insect Biochemistry and Physiology
Ana R Cabrera, Paul D Shirk, Peter E A Teal
A novel feeding protocol for delivery of bio-active agents to Varroa mites was developed by providing mites with honey bee larva hemolymph supplemented with cultured insect cells and selected materials delivered on a fibrous cotton substrate. Mites were starved, fed on treated hemolymph to deliver selected agents and then returned to bee larvae. Transcript levels of two reference genes, actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), as well as for nine selected genes involved in reproductive processes showed that the starvation and feeding protocol periods did not pose a high level of stress to the mites as transcript levels remained comparable between phoretic mites and those completing the protocol...
2017: PloS One
M N Abbas, S Kausar, Y-X Sun, Y Sun, L Wang, C Qian, G-Q Wei, B-J Zhu, C-L Liu
The E2F transcription factor family is distributed widely in eukaryotes and has been well studied among mammals. In the present study, the E2F transcription factor 4 (E2F4) gene was isolated from fat bodies of Antheraea pernyi and sequenced. E2F4 comprised a 795 bp open reading frame encoding a deduced amino acid sequence of 264 amino acid residues. The recombinant protein was expressed in Escherichia coli (Transetta DE3), and anti-E2F4 antibodies were prepared. The deduced amino acid sequence displayed significant homology to an E2F4-like protein from Bombyx mori L...
April 24, 2017: Bulletin of Entomological Research
Kun Yu, Fei Lu, Qing Li, Yini Zou, Yang Xiao, Bitao Lu, Jiawei Liu, Fangying Dai, Dayang Wu, Guangqian Lan
Silk fibroin materials have shown some success in wound dressing applications; however, their use for this purpose remains limited by a complex production process and wasted sericin. In the present study, Bombyx mori cocoon materials are used because the protective function of the silkworm cocoon resembles the manner in which the skin protects the human body. A series of silkworm cocoon sol-gel film (SCSF) wound dressings are prepared by immersion in a CaCl2-ethanol-H2O solution for different treatment times...
April 20, 2017: International Journal of Biological Macromolecules
M Qu, Y Ren, Y Liu, Q Yang
Chitin deacetylation is required to make the cuticle rigid and compact through chitin chain crosslinking. Thus it is presumed that specialized proteins are required to bind deacetylated chitin chains together. However, deacetylated-chitin binding proteins have not ever been reported. In a previous work, six cuticular proteins analogous to peritrophin 3 (CPAP3s) were found to be abundant in the moulting fluid of Bombyx mori. In this study, these BmCPAP3s (BmCPAP3-A1, BmCPAP3-A2, BmCPAP3-B, BmCPAP3-C, BmCPAP3-D1 and BmCPAP3-D2) were cloned and expressed in Escherichia coli and purified using metal-chelating affinity chromatography...
April 22, 2017: Insect Molecular Biology
Ottavia Romoli, Alessio Saviane, Andrea Bozzato, Paola D'Antona, Gianluca Tettamanti, Andrea Squartini, Silvia Cappellozza, Federica Sandrelli
The domesticated silkworm Bombyx mori has an innate immune system, whose main effectors are the antimicrobial peptides (AMPs). Silkworm strains are commonly grouped into four geographical types (Japanese, Chinese, European and Tropical) and are generally characterised by a variable susceptibility to infections. To clarify the genetic and molecular mechanisms on which the different responses to infections are based, we exposed one silkworm strain for each geographical area to oral infections with the silkworm pathogens Enterococcus mundtii or Serratia marcescens...
April 21, 2017: Scientific Reports
R S Sajeevan, Karaba N Nataraja, K S Shivashankara, N Pallavi, D S Gurumurthy, M B Shivanna
Mulberry (Morus species) leaf is the sole food for monophagous silkworms, Bombyx mori L. Abiotic stresses such as drought, salinity, and high temperature, significantly decrease mulberry productivity and post-harvest water loss from leaves influence silkworm growth and cocoon yield. Leaf surface properties regulate direct water loss through the cuticular layer. Leaf surface waxes, contribute for cuticular resistance and protect mesophyll cells from desiccation. In this study we attempted to overexpress AtSHN1, a transcription factor associated with epicuticular wax biosynthesis to increase leaf surface wax load in mulberry...
2017: Frontiers in Plant Science
Pingyang Wang, Zhiyong Qiu, Dingguo Xia, Shunming Tang, Xingjia Shen, Qiaoling Zhao
A new purple quail-like (q-lp) mutant found from the plain silkworm strain 932VR has pigment dots on the epidermis similar to the pigment mutant quail (q). In addition, q-lp mutant larvae are inactive, consume little and grow slowly, with a high death rate and other developmental abnormalities. Pigmentation of the silkworm epidermis consists of melanin, ommochrome and pteridine. Silkworm development is regulated by ecdysone and juvenile hormone. In this study, we performed RNA-Seq on the epidermis of the q-lp mutant in the 4th instar during molting, with 932VR serving as the control...
2017: PloS One
Guohui Li, Qian Zhou, Lipeng Qiu, Qin Yao, Keping Chen, Qi Tang, Zhaoyang Hu
Bm-SP142 is a 35 kDa protease in the silkworm, but its exact functions remain unknown. In this study, sequence alignment revealed that the His-Asp-Ser catalytic triad is embedded in the TAAHC-DIAL-GDSGGP sequence motif, establishing Bm-SP142 as a serine protease. Soluble recombinant GST-BmSP142 was expressed and purified, and serine protease activity was confirmed in vitro. RT-qPCR results indicated that Bm-SP142 was mainly expressed in the middle part of the silkworm midgut, and Bm-SP142 transcripts were significantly up-regulated at 24 hours post infection (hpi) in BmBDV-resistant strains (798) inoculated with BmBDV and BmNPV-resistant strains (NB) inoculated with BmNPV, but not in BmBDV-susceptible strains (306)...
2017: PloS One
Sumiharu Nagaoka, Maiko Asagoshi, Keita Kato, Yuki Takata
A trypsin-like protease called initiatorin is known to initiate sperm motility in the silkworm, Bombyx mori, but little is known about the signaling events leading to sperm flagellar beating. The aim of this study was to investigate whether this mechanism of sperm motility activation involves the signaling transmitter nitric oxide (NO). NO is produced from the amino acid L-arginine by the enzyme action of nitric oxide synthase (NOS; EC Simple treatment of quiescent sperm with an NO donor (SNAP or NOC7) in vitro did not lead to activation of motility...
April 13, 2017: Insect Biochemistry and Molecular Biology
Keita Mori, Hirotsugu Hamada, Takafumi Ogawa, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
Transient gene expression allows a rapid production of diverse recombinant proteins in early-stage preclinical and clinical developments of biologics. Insect cells have proven to be an excellent platform for the production of functional recombinant proteins. In the present study, the production of an antibody Fab fragment by transient gene expression in lepidopteran insect cells was investigated. The DNA fragments encoding heavy-chain (Hc; Fd fragment) and light-chain (Lc) genes of an Fab fragment were individually cloned into the plasmid vector pIHAneo, which contained the Bombyx mori actin promoter downstream of the B...
April 11, 2017: Journal of Bioscience and Bioengineering
Asfa Ali, Venugopal Reddy Bovilla, Danti Kumari Mysarla, Prasanthi Siripurapu, Rashmi U Pathak, Bhakti Basu, Anitha Mamillapalli, Santanu Bhattacharya
Bombyx mori (B. mori) is important due to its major role in the silk production. Though DNA binding ligands often influence gene expression, no attempt has been made to exploit their use in sericulture. The telomeric heterochromatin of B. mori is enriched with 5'-TTAGG-3' sequences. These sequences were also found to be present in several genes in the euchromatic regions. We examined three synthetic oligopyrrole carboxamides that target 5'-TTAGG-3' sequences in controlling the gene expression in B. mori. The ligands did not show any defect or feeding difference in the larval stage, crucial for silk production...
April 11, 2017: Scientific Reports
Zhan-Qi Dong, Nan Hu, Fei-Fan Dong, Ting-Ting Chen, Ya-Ming Jiang, Peng Chen, Cheng Lu, Min-Hui Pan
Research on molecular mechanisms that viruses use to regulate the host apparatus is important in virus infection control and antiviral therapy exploration. Our previous research showed that the Bombyx mori nucleopolyhedrovirus (BmNPV) LEF-11 localized to dense regions of the cell nucleus and is required for viral DNA replication. Herein, we examined the mechanism of LEF-11 on BmNPV multiplication and demonstrated that baculovirus LEF-11 interacts with Bombyx mori ATAD3A and HSPD1 (HSP60) protein. Furthermore, we showed that LEF-11 has the ability to induce and up-regulate the expression of ATAD3A and HSPD1, phenomena that were both reversed upon knockdown of lef-11...
April 10, 2017: Scientific Reports
Hui Tao, Xue Li, Jian-Feng Qiu, Wen-Zhao Cui, Yang-Hu Sima, Shi-Qing Xu
Abnormalities in the circadian clock system are known to affect the body's metabolic functions, though the molecular mechanisms responsible remain uncertain. In this study, we achieved continuous knockdown of B. mori Period (BmPer) gene expression in the B. mori ovary cell line (BmN), and generated a Per-KD B. mori model with developmental disorders including small individual cells and slow growth. We conducted cell metabolomics assays by gas chromatography/liquid chromatography-mass spectrometry and showed that knockdown of BmPer gene expression resulted in significant inhibition of glycometabolism...
April 10, 2017: Scientific Reports
Taoyi Mai, Shuna Chen, Xianyu Lin, Xiaojuan Zhang, Xiaopeng Zou, Qili Feng, Sichun Zheng
Metamorphosis is an essential physiological process in insects. This process is triggered by 20-hydroxyecydsone (20E). Lebocin, an antimicrobial peptide of Lepidoptera insects, was significantly up-regulated in the midgut, but not in the fat body of Bombyx mori during metamorphosis. In this study, the expression regulation of lebocin in B. mori midgut was studied. The results showed that B. mori lebocin and its activator BmEts were not responsive to bacterial infection in the midgut, instead, the expression of both genes was up-regulated by 20E treatment...
April 6, 2017: Developmental and Comparative Immunology
Xiao-Fang Tang, Xiang-Yun Chen, Chun-Dong Zhang, Yao-Feng Li, Tai-Hang Liu, Xiao-Lin Zhou, La Wang, Qian Zhang, Peng Chen, Cheng Lu, Min-Hui Pan
DNA replication is rigorously controlled in cells to ensure that the genome duplicates exactly once per cell cycle. Geminin is a small nucleoprotein, which prevents DNA rereplication by directly binding to and inhibiting the DNA replication licensing factor, Cdt1. In this study, we have identified 2 Geminin genes, BmGeminin1 and BmGeminn2, in silkworm, Bombyx mori. These genes contain the Geminin conserved coiled-coil domain and are periodically localized in the nucleus during the S-G2 phase but are degraded at anaphase in mitosis...
April 5, 2017: Cell Cycle
Chaoshan Han, Enxiang Chen, Guanwang Shen, Peng Zhixin, Yinying Xu, Haiyan Zhang, Hongling Liu, Yandi Zhang, Jinxin Wu, Ying Lin, Qingyou Xia
Vitellogenin receptor (VgR), a member of the low-density lipoprotein receptor (LDLR) family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, Bombyx mori VgR (BmVgR) has been shown to interact with Bombyx mori storage protein (BmSP1) in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with Bombyx mori Vg (BmVg) and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells...
April 4, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Michael J Landry, Matthew B Applegate, Oleksandr S Bushuyev, Fiorenzo G Omenetto, David L Kaplan, Mark Cronin-Golomb, Christopher J Barrett
Azobenzene modification of Bombyx mori silkworm silk creates a photo-responsive 'azosilk' biomaterial, allowing for 3D laser patterning. Written regions fluoresce, and become fluid-filled raised 'micro-blisters' with a 10-fold photo-softening effect of the modulus. Patterning is facile and versatile, with potential applications as soft tunable materials for dynamic cell guidance and microfluidics.
April 19, 2017: Soft Matter
Lan Su, Congrui Xu, Chuangang Cheng, Chengfeng Lei, Xiulian Sun
Dendrolimus punctatus cypovirus (DpCPV) is an important pathogen of D. punctatus, but little is known about the mechanisms of DpCPV infection. Here, we investigated the effects of VP3, VP4 and VP5 structural proteins on the viral invasion. Both the C-terminal of VP3 (methyltransferase (MTase) domain) and VP4 (A-spike) bound to Spodoptera exigua midgut brush border membrane vesicles (BBMVs) in a dose-dependent manner, and the binding was inhibited by purified DpCPV virions. Importantly, anti-MTase and anti-VP4 antibodies inhibited viral binding to S...
April 1, 2017: Viruses
Xue-Yang Wang, Hai-Zhong Yu, Jia-Ping Xu, Shang-Zhi Zhang, Dong Yu, Ming-Hui Liu, Lin-Ling Wang
The molecular mechanism of silkworm resistance to Bombyx mori nucleopolyhedrovirus (BmNPV) infection remains largely unclear. Accumulating evidence suggests that subcellular fractionation combined with proteomics is an ideal technique to analyse host antiviral mechanisms. To clarify the anti-BmNPV mechanism of the silkworm, the near-isogenic line BC9 (resistant strain) and the recurrent parent P50 (susceptible strain) were used in a comparative subcellular proteomics study. Two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry (MS) was conducted on proteins extracted from the cytosol, mitochondria, and microsomes of BmNPV-infected and control larval midguts...
March 31, 2017: Scientific Reports
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