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https://www.readbyqxmd.com/read/29337221/venom-characterization-of-the-amazonian-scorpion-tityus-metuendus
#1
C V F Batista, J G Martins, R Restano-Cassulini, F I V Coronas, F Z Zamudio, R Procópio, L D Possani
The soluble venom from the scorpion Tityus metuendus was characterized by various methods. In vivo experiments with mice showed that it is lethal. Extended electrophysiological recordings using seven sub-types of human voltage gated sodium channels (hNav1.1 to 1.7) showed that it contains both α- and β-scorpion toxin types. Fingerprint analysis by mass spectrometry identified over 200 distinct molecular mass components. At least 60 sub-fractions were recovered from HPLC separation. Five purified peptides were sequenced by Edman degradation, and their complete primary structures were determined...
January 11, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29336144/nanostructured-peptidotoxins-as-natural-pro-oxidants-induced-cancer-cell-death-via-amplification-of-oxidative-stress
#2
Hongzhi Qiao, Dong Fang, Lei Zhang, Xiaochen Gu, Yin Lu, Minjie Sun, Chunmeng Sun, Qineng Ping, Junsong Li, Zhipeng Chen, Jun Chen, Lihong Hu, Liuqing Di
Melittin (Mel), one of the host defense peptides (HDPs) derived from venom of honeybees, demonstrates substantial anticancer properties, which is considered attributed to augmenting reactive oxygen species (ROS) generation. However, little has been reported on its pro-oxidation capacity in cancer oxidation therapy. In this study, a ROS amplifying nanodevice was fabricated through direct complexation of two natural pro-oxidants, Mel and condensed epigallocatechin gallate (pEGCG). The obtained nanocomplex (NC) was further covered with phenylboronic acid derivatized hyaluronic acid (pHA) through ROS-responsive boronate ester coordination bond to produce pHA-NC...
January 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29333393/effect-of-monascus-fermented-soybean-extracts-on-antioxidant-and-skin-aging-related-enzymes-inhibitory-activities
#3
Yoo-Jeong Jin, Young-Hee Pyo
We investigated the in vitro inhibitory activity against skin aging-related enzymes and antioxidant activity of Monascus-fermented soybean extracts (MFSEs) obtained by using different solvents. The highest Trolox equivalent (TE) antioxidant capacity (3.13±0.06 mM TE/g) and oxygen radical absorbance capacity (2.79±0.09 mM TE/g) of MFSEs were evaluated for the methanol and 80% ethanol extracts, respectively. The antioxidant capacities increased with increasing concentration (0.5~50 mg/mL). In addition, the methanol and 80% ethanol extracts showed an effective inhibition against tyrosinase, hyaluronidase, and elastase compared with those of acetone and hot water extracts (P<0...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29322079/fibroblast-and-keratinocyte-gene-expression-following-exposure-to-extracts-of-neem-plant-azadirachta-indica
#4
Takao Someya, Katsura Sano, Kotaro Hara, Yoshimasa Sagane, Toshihiro Watanabe, R G S Wijesekara
This data article provides gene expression profiles, determined by using real-time PCR, of fibroblasts and keratinocytes treated with 0.01% and 0.001% extracts of neem plant (Azadirachta indica), local name "Kohomba" in Sri Lanka, harvested in Sri Lanka. For fibroblasts, the dataset includes expression profiles for genes encoding hyaluronan synthase 1 (HAS1), hyaluronan synthase 2 (HAS2), hyaluronidase-1 (HYAL1), hyaluronidase-2 (HYAL2), versican, aggrecan, CD44, collagen, type I, alpha 1 (COL1A1), collagen, type III, alpha 1 (COL3A1), collagen, type VII, alpha 1 (COL7A1), matrix metalloproteinase 1 (MMP1), acid ceramidase, basic fibroblast growth factor (bFGF), fibroblast growth factor-7 (FGF7), vascular endothelial growth factor (VEGF), interleukin-1 alpha (IL-1α), cyclooxygenase-2 (cox2), transforming growth factor beta (TGF-β), and aquaporin 3 (AQP3)...
February 2018: Data in Brief
https://www.readbyqxmd.com/read/29318879/intelligent-mos2-nanotheranostic-for-targeted-and-enzyme-ph-nir-responsive-drug-delivery-to-overcome-cancer-chemotherapy-resistance-guided-by-pet-imaging
#5
Xinghua Dong, Wenyan Yin, Xiao Zhang, Shuang Zhu, Xiao He, Jie Yu, Jiani Xie, Zhao Guo, Liang Yan, Xiangfeng Liu, Qing Wang, Zhanjun Gu, Yuliang Zhao
Chemotherapy resistance remains a major hurdle for cancer therapy in clinic due to poor cellular uptake, insufficient intracellular release of drugs. Herein, an intelligent, multifunctional MoS2 nanotheranostic (MoS2-PEI-HA) ingeniously decorating with biodegradable hyaluronic acid (HA) assisted by polyethyleneimine (PEI) is reported to combat drug-resistant breast cancer (MCF-7-ADR) after loading with chemotherapy drug doxorubicin (DOX). The HA can not only target CD44-overexpressing MCF-7-ADR, but also be degraded by hyaluronidase (HAase) that is concentrated in the tumor microenvironment and thus to accelerate doxorubicin (DOX) release...
January 10, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29316635/skin-protective-effect-of-epigallocatechin-gallate
#6
Eunji Kim, Kyeonghwan Hwang, Jongsung Lee, Sang Yun Han, Eun-Mi Kim, Junseong Park, Jae Youl Cho
Epigallocatechin gallate (EGCG) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated EGCG as a cosmetic constituent, the skin hydration effect of EGCG is poorly understood. We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase (HAS) and hyaluronidase (HYAL) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis...
January 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29305080/de-novo-transcriptomic-analysis-of-the-venomous-glands-from-the-scorpion-heterometrus-spinifer-revealed-unique-and-extremely-high-diversity-of-the-venom-peptides
#7
Yuchen Deng, Jiawei Gu, Zhenpeng Yan, Mengdi Wang, Chaoqun Ma, Jiafeng Zhang, Guixian Jiang, Mengxiao Ge, Shuogui Xu, Zheng Xu, Liang Xiao
Scorpion, as an ancient species, has been widely used on dozens of human diseases in traditional Chinese Medicine. Although the scorpion venom from the Buthidae family with the potent toxicity attracts more interests, toxins from the non-Buthidae family draw great attention as well because of its abundance and complexity even without harm to mammals. Moreover, several toxic components of scorpion venom have been identified as valuable scaffolds for the drug design and development. Using the Next Generation Sequencing (NGS) technique, here we reported the transcriptome of the venomous glands of Heterometrus spinifer, a non-Buthidae scorpion that only a few toxic and complete components have been identified known-to-date...
January 2, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29302970/tailor-making-fluorescent-hyaluronic-acid-microgels-via-combining-microfluidics-and-photoclick-chemistry-for-sustained-and-localized-delivery-of-herceptin-in-tumor
#8
Jing Chen, Ke Huang, Qijun Chen, Chao Deng, Jian Zhang, Zhiyuan Zhong
Antibody therapeutics though representing a most used biomedicine suffers from poor in vivo stability, rapid degradation, and frequent injections. Here, we report that fluorescent hyaluronic acid microgels (HMGs) tailor-made by combining microfluidics and "tetrazole-alkene" photoclick chemistry enable sustained and localized delivery of Herceptin in ovarian tumor. HMGs were obtained with defined size (25-50 μm), narrow size distribution, high stability, and strong green fluorescence. Notably, HMGs exhibited a remarkably high loading of proteins like Herceptin and IgG with a loading efficiency exceeding 90% at a theoretical protein loading content of 30 wt...
January 5, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29299202/the-role-of-tsg-6-and-uroplakin-iii-in-bladder-pain-syndrome-interstitial-cystitis-in-rats-and-humans
#9
Yi-Song Lv, Rui Gao, Qing-Ming Lin, Tao Jiang, Qin Chen, Song-Xi Tang, Hou-Ping Mao, Hui-Liang Zhou, Lin-Sheng Cao
Objectives: We investigated the relationship between the expression of tumor necrosis factor-inducible gene 6 (TSG-6) with inflammation and integrity of the bladder epithelium in the bladder tissues of patients with bladder pain syndrome/interstitial cystitis (BPS/IC) and the mechanism of action using a rat model of BPS/IC. Materials and Methods: Expression of TSG-6 and uroplakin III was determined by immuno- histochemistry of bladder biopsy samples from control human subjects and patients with verified BPS/IC...
November 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29276289/a-case-of-metronidazole-injection-infiltration-without-sequelae
#10
Andrew M North, Justin M Yee
Purpose: This report describes a case of metronidazole injection infiltration which contrasts the sole other case report in the literature at this time, as the patient described herein experienced mild signs and symptoms with prompt resolution and no significant sequelae. Summary: The patient experienced metronidazole injection infiltration during administration open to gravity via an 18-gauge peripheral catheter in the left brachial vein. The site was examined at bedside within approximately 30 minutes of the incident and was noted to be slightly edematous, erythemic, and painful in terms of a 5...
September 2017: Hospital Pharmacy
https://www.readbyqxmd.com/read/29247734/human-chitotriosidase-does-not-catabolize-hyaluronan
#11
Ben Danielson, Che-Hong Chen, Gernot Kaber, Daria Mochly-Rosen, Kevin Grimes, Robert Stern, Paul L Bollyky
Humans express an enzyme that degrades chitin, called chitotriosidase, despite the fact that we do not produce chitin. One possible explanation for this is that chitinase also degrades hyaluronan, a polysaccharide that is abundant in human tissues and shares structural attributes in common with chitinase. The objective of this study was to determine whether human chitotriosidase is capable of hydrolyzing hyaluronan. Hyaluronan of various sizes under a range of pH conditions displayed no degradation when incubated with various chitinases over a period of 5 days, while commercial hyaluronidase readily digested the hyaluronan...
December 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29244093/commentary-on-efficacy-of-retrobulbar-hyaluronidase-injection-for-vision-loss-resulting-from-hyaluronic-acid-filler-embolization
#12
Claudio DeLorenzi
No abstract text is available yet for this article.
December 13, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29244079/a-non-hemolytic-group-b-streptococcus-strain-exhibits-hypervirulence
#13
Claire Gendrin, Jay Vornhagen, Blair Armistead, Pallavi Singh, Christopher Whidbey, Sean Merillat, David Knupp, Robert Parker, Lisa M Rogers, Phoenicia Quach, Lakshminarayan M Iyer, L Aravind, Shannon D Manning, David M Aronoff, Lakshmi Rajagopal
Group B Streptococci (GBS) are gram-positive bacteria that are a leading cause of neonatal infections. Most invasive isolates are -hemolytic and hemolytic activity is critical for GBS virulence. While non-hemolytic GBS strains are occasionally isolated, they are often thought to be virulence attenuated. Here, we show that a non-hemolytic GBS strain (GB37) isolated from a septic neonate exhibits hypervirulence. Substitution of tryptophan to leucine (W297L) in the sensor histidine kinase CovS results in constitutive kinase signaling, leading to decreased hemolysis and increased activity of the GBS hyaluronidase, HylB...
December 13, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29238458/bacteriocinogenic-potential-and-virulence-traits-of-enterococcus-faecium-and-e-faecalis-isolated-from-human-milk
#14
Soodabeh Khalkhali, Naheed Mojgani
Background and Objectives: Human milk is a continuous supply of Lactic Acid bacteria (LAB), including enterococci with probiotic potentials. The aim of this study was to analyze two Enterococcus species, isolated from human milk for their probiotic potential, bacteriocin producing ability and virulence traits. Materials and Methods: Enterococcus faecium TA0033 and E. faecalis TA102 were tested for acid and bile tolerance, survival in simulated gastric and intestinal conditions...
August 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29236224/two-new-aromatic-glycosides-elengiosides-a-and-b-from-the-flowers-of-mimusops-elengi
#15
Toshio Morikawa, Yoshiaki Manse, Mika Koda, Saowanee Chaipech, Yutana Pongpiriyadacha, Osamu Muraoka, Kiyofumi Ninomiya
Two new aromatic glycosides, elengiosides A (1) and B (2), were isolated from the methanolic extract of the flowers of Mimusops elengi (Sapotaceae) together with 26 known compounds. Their stereostructures were elucidated based on their spectroscopic properties and chemical evidence. Among the isolates, a phenylethanoid glycoside, undatuside C (14), was found to exhibit hyaluronidase inhibitory activity.
December 13, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29236055/hyaluronan-production-by-renomedullary-interstitial-cells-influence-of-endothelin-angiotensin-ii-and-vasopressin
#16
Sara Stridh, Fredrik Palm, Tomoko Takahashi, Mayumi Ikegami-Kawai, Malou Friederich-Persson, Peter Hansell
The content of hyaluronan (HA) in the interstitium of the renal medulla changes in relation to body hydration status. We investigated if hormones of central importance for body fluid homeostasis affect HA production by renomedullary interstitial cells in culture (RMICs). Simultaneous treatment with vasopressin and angiotensin II (Ang II) reduced HA by 69%. No change occurred in the mRNA expressions of hyaluronan synthase 2 (HAS2) or hyaluronidases (Hyals), while Hyal activity in the supernatant increased by 67% and CD44 expression reduced by 42%...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235360/halo-109-301-a-phase-iii-trial-of-pegph20-with-gemcitabine-and-nab-paclitaxel-in-hyaluronic-acid-high-stage-iv-pancreatic-cancer
#17
Gary J Doherty, Margaret Tempero, Pippa G Corrie
The outlook for patients with advanced pancreatic cancer remains poor, despite significant advances in our understanding of pancreatic tumor biology. One emerging theme highlights the distinct composition of the pancreatic tumor microenvironment. Hyaluronic acid is a hydrophilic glycosaminoglycan whose production within the tumor leads to increased interstitial tumor pressure, thereby limiting the access of potentially effective circulating anticancer drugs via reduced tumor perfusion. PEGylated rHuPH20 is a multiply PEGylated recombinant human hyaluronidase that has shown promising efficacy in preclinical models and early phase clinical trials in pancreatic cancer patients...
October 23, 2017: Future Oncology
https://www.readbyqxmd.com/read/29231858/anti-allergic-activity-of-monoacylated-ascorbic-acid-2-glucosides
#18
Kaori Miura, Yuta Morishita, Hiroaki Matsuno, Yusuke Aota, Hideyuki Ito, Akihiro Tai
2-O-α-d-Glucopyranosyl-l-ascorbic acid (AA-2G) is one of the stable ascorbic acid (AA) derivatives known as provitamin C agents. We have previously synthesized two types of monoacylated derivatives of AA-2G, 6-O-acyl-2-O-α-d-glucopyranosyl-l-ascorbic acids having a straight-acyl chain of varying length from C₄ to C18 (6-sAcyl-AA-2G) and a branched-acyl chain of varying length from C₆ to C16 (6-bAcyl-AA-2G) in order to improve the bioavailability of AA-2G. In this study, 6-sAcyl-AA-2G and 6-bAcyl-AA-2G per se showed the inhibitory effects on hyaluronidase activity and degranulation...
December 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29229728/cas9-enhances-bacterial-virulence-by-repressing-the-regr-transcriptional-regulator-in-streptococcus-agalactiae
#19
Ke Ma, Qing Cao, Su Luo, Zhaofei Wang, Guangjin Liu, Chengping Lu, Yongjie Liu
Clustered regularly interspaced palindromic repeats (CRISPR) and its associated cas genes have been demonstrated to regulate self-genes and virulence in many pathogens. In this study, we found that inactivation of cas9 caused reduced adhesion and intracellular survival of the piscine Streptococcus agalactiae strain GD201008-001 and significantly decreased the virulence of this strain in zebrafish and mice. Further investigation indicated that the regR transcriptional regulator was upregulated in the Δcas9 mutant...
December 11, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29213354/chemical-composition-and-pharmacological-effects-of-geopropolis-produced-by-melipona-quadrifasciata-anthidioides
#20
Cintia Miranda Dos Santos, Jaqueline Ferreira Campos, Helder Freitas Dos Santos, José Benedito Perrella Balestieri, Denise Brentan Silva, Kely de Picoli Souza, Carlos Alexandre Carollo, Leticia M Estevinho, Edson Lucas Dos Santos
Stingless bees produce geopropolis, which is popularly described for its medicinal properties, but for which few scientific studies have demonstrated pharmacological effects. The objective of this study was to investigate the chemical composition of the geopropolis of Melipona quadrifasciata anthidioides and to evaluate its antioxidant, antimutagenic, anti-inflammatory, and antimicrobial activities. The composition of the hydroethanolic extract of geopropolis (HEG) included di- and trigalloyl and phenylpropanyl heteroside derivatives, flavanones, diterpenes, and triterpenes...
2017: Oxidative Medicine and Cellular Longevity
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