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hyaluronidase

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https://www.readbyqxmd.com/read/28439731/il-10-improves-cardiac-remodeling-after-myocardial-infarction-by-stimulating-m2-macrophage-polarization-and-fibroblast-activation
#1
Mira Jung, Yonggang Ma, Rugmani Padmanabhan Iyer, Kristine Y DeLeon-Pennell, Andriy Yabluchanskiy, Michael R Garrett, Merry L Lindsey
Inflammation resolution is important for scar formation following myocardial infarction (MI) and requires the coordinated actions of macrophages and fibroblasts. In this study, we hypothesized that exogenous interleukin-10 (IL-10), an anti-inflammatory cytokine, promotes post-MI repair through actions on these cardiac cell types. To test this hypothesis, C57BL/6J mice (male, 3- to 6-month old, n = 24/group) were treated with saline or IL-10 (50 μg/kg/day) by osmotic mini-pump infusion starting at day (d) 1 post-MI and sacrificed at d7 post-MI...
May 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28433637/inhibition-of-pro-inflammatory-enzymes-by-medicinal-plants-from-the-argentinean-highlands-puna
#2
Romina Torres-Carro, María Inés Isla, Samanta Thomas-Valdes, Felipe Jiménez-Aspee, Guillermo Schmeda-Hirschmann, María Rosa Alberto
ETHNOPHARMACOLOGICAL RELEVANCE: Human groups in the Argentinean Andes highlands (Puna) selected native plants as anti-inflammatory agents. The indications of use are mainly to relieve pain, in infusions, ethanolic extracts or plasters. AIM OF THE STUDY: The objective of the study was to assess the effect of hydroalcoholic extracts from native highland plants as anti-inflammatory according to the traditional indications of use. The chemical profile of the three most active species was analyzed by HPLC-ESI-MS to get in insight into the constituents and the effects observed, according to the ethnopharmacological information...
April 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28428153/development-of-polyol-responsive-antibody-mimetics-for-single-step-protein-purification
#3
Richard J Suderman, Daren A Rice, Shane D Gibson, Eric J Strick, David M Chao
The purification of functional proteins is a critical pre-requisite for many experimental assays. Immunoaffinity chromatography, one of the fastest and most efficient purification procedures available, is often limited by elution conditions that disrupt structure and destroy enzymatic activity. To address this limitation, we developed polyol-responsive antibody mimetics, termed nanoCLAMPs, based on a 16 kDa carbohydrate binding module domain from Clostridium perfringens hyaluronidase. nanoCLAMPs bind targets with nanomolar affinity and high selectivity yet release their targets when exposed to a neutral polyol-containing buffer, a composition others have shown to preserve quaternary structure and enzymatic activity...
April 17, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28423120/primary-cultures-of-mouse-small-intestinal-epithelial-cells-using-the-dissociating-enzyme-type-i-collagenase-and-hyaluronidase
#4
H J Ren, C L Zhang, R D Liu, N Li, X G Li, H K Xue, Y Guo, Z Q Wang, J Cui, L Ming
The epithelium is a highly dynamic system, which plays a crucial role in the homeostasis of the intestinal tract. However, studies on the physiological and pathophysiological functions of intestinal epithelial cells (IECs) have been hampered due to lack of normal epithelial cell models. In the present study, we established a reproducible method for primary culture of mouse IECs, which were isolated from the viable small intestinal crypts of murine fetuses (on embryonic day 19), using type I collagenase and hyaluronidase in a short span of time (≤20 min)...
April 13, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28421387/pharmacokinetics-of-monoclonal-antibodies-and-fc-fusion-proteins
#5
REVIEW
Liming Liu
There are many factors that can influence the pharmacokinetics (PK) of a mAb or Fc-fusion molecule with the primary determinant being FcRn-mediated recycling. Through Fab or Fc engineering, IgG-FcRn interaction can be used to generate a variety of therapeutic antibodies with significantly enhanced half-life or ability to remove unwanted antigen from circulation. Glycosylation of a mAb or Fc-fusion protein can have a significant impact on the PK of these molecules. mAb charge can be important and variants with pI values of 1-2 unit difference are likely to impact PK with lower pI values being favorable for a longer half-life...
April 19, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28411450/activatable-near-infrared-dye-conjugated-hyaluronic-acid-based-nanoparticles-as-a-targeted-theranostic-agent-for-enhanced-fluorescence-ct-photoacoustic-imaging-guided-photothermal-therapy
#6
Xiaolong Liang, Lei Fang, Xiaoda Li, Xu Zhang, Fan Wang
Targeted theranostic nano-system integrating functions of both diagnosis and therapy shows great potential for improving diagnosis and therapeutic efficacy. Herein, multifunctional nanoparticle based on activatable hyaluronic acid (HA) conjugating two near-infrared (NIR) dyes of Cy5.5 and IR825 was successfully designed and fabricated, and simultaneously used as a carrier for encapsulating perfluorooctylbromide (PFOB). In this system, PFOB showed good capability to absorb the X-rays, Cy5.5 on the outer surface acted as a fluorescent dye activatable by hyaluronidases (Hyals) in the tumor, and IR825 in the core as a photothermal agent...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28405129/effect-of-addition-of-fentanyl-and-clonidine-to-local-anesthetic-solution-in-peribulbar-block
#7
Poonam Nehra, Vrinda Oza, Vandana Parmar, Pooja Fumakiya
OBJECTIVE: To compare the effect of addition of fentanyl and clonidine as adjuvants to bupivacaine and lignocaine in peribulbar block. METHODS: The study was conducted on 105 adult patients of either sex, of ASA grade I and II undergoing ophthalmic surgeries. Patients were randomly divided into 3 groups of 35 each. All the patients were given peribulbuar block with 5ml lignocaine 2% +3 ml bupivacaine 0.5% +1 ml hyaluronidase (250 IU). In addition to this 1 ml normal saline was added to Group S, 25 μg fentanyl to Group F and 25 μg clonidine to Group C...
January 2017: Journal of Pharmacology & Pharmacotherapeutics
https://www.readbyqxmd.com/read/28399917/metformin-and-sulodexide-restore-cardiac-microvascular-perfusion-capacity-in-diet-induced-obese-rats
#8
Judith van Haare, M Eline Kooi, Jurgen W G E van Teeffelen, Hans Vink, Jos Slenter, Hanneke Cobelens, Gustav J Strijkers, Dennis Koehn, Mark J Post, Marc van Bilsen
BACKGROUND: Disturbances in coronary microcirculatory function, such as the endothelial glycocalyx, are early hallmarks in the development of obesity and insulin resistance. Accordingly, in the present study myocardial microcirculatory perfusion during rest and stress was assessed following metformin or sulodexide therapy in a rat model of diet-induced obesity. Additionally, the effect of degradation of the glycocalyx on myocardial perfusion was assessed in chow-fed rats. METHODS: Rats were fed a high fat diet (HFD) for 8 weeks and were divided into a group without therapy, and groups that received the anti-diabetic drug metformin or the glycocalyx-stabilizing drug sulodexide in their drinking water during the last 4 weeks of the feeding period...
April 11, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28392982/comparative-venom-gland-transcriptomics-of-naja-kaouthia-monocled-cobra-from-malaysia-and-thailand-elucidating-geographical-venom-variation-and-insights-into-sequence-novelty
#9
Kae Yi Tan, Choo Hock Tan, Lawan Chanhome, Nget Hong Tan
BACKGROUND: The monocled cobra (Naja kaouthia) is a medically important venomous snake in Southeast Asia. Its venom has been shown to vary geographically in relation to venom composition and neurotoxic activity, indicating vast diversity of the toxin genes within the species. To investigate the polygenic trait of the venom and its locale-specific variation, we profiled and compared the venom gland transcriptomes of N. kaouthia from Malaysia (NK-M) and Thailand (NK-T) applying next-generation sequencing (NGS) technology...
2017: PeerJ
https://www.readbyqxmd.com/read/28372836/macroalga-padina-pavonica-water-extracts-obtained-by-pressurized-liquid-extraction-and-microwave-assisted-extraction-inhibit-hyaluronidase-activity-as-shown-by-capillary-electrophoresis
#10
Syntia Fayad, Reine Nehmé, Mona Tannoury, Eric Lesellier, Chantal Pichon, Philippe Morin
Hyaluronidase degrades hyaluronic acid, the principal component of the extracellular matrix. Inhibition of this enzyme is thus expected to hinder skin aging. Brown alga Padina pavonica activity toward hyaluronidase was evaluated using capillary electrophoresis (CE)-based enzymatic assays. This green technique allows evaluation of the biological activity of the natural material in an economic manner. Pressurized liquid extraction (PLE), microwave assisted extraction (MAE), supercritical fluid extraction and electroporation extraction techniques were used...
March 20, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28367600/microfluidic-processing-of-synovial-fluid-for-cytological-analysis
#11
John C Krebs, Yunus Alapan, Barbara A Dennstedt, Glenn D Wera, Umut A Gurkan
Cytological analysis of synovial fluid is widely used in the clinic to assess joint health and disease. However, in general practice, only the total number of white blood cells (WBCs) are available for cytologic evaluation of the joint. Moreover, sufficient volume of synovial aspirates is critical to run conventional analyses, despite limited volume of aspiration that can normally be obtained from a joint. Therefore, there is a lack of consistent and standardized synovial fluid cytological tests in the clinic...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28349704/lc-esi-ms-ms-profiling-of-phenolics-in-the-leaves-of-eleutherococcus-senticosus-cultivated-in-the-west-europe-and-anti-hyaluronidase-and-anti-acetylcholinestarase-activities
#12
Rafał Kuźniewski, Daniel Załuski, Marta Olech, Piotr Banaszczak, Renata Nowak
Neither secondary metabolites of the spring leaves nor the autumn leaves of Eleutherococcus senticosus species cultivated in Poland, or the bioactivity are known. The richest in polyphenols was the autumn leaves (171.1 mg/g DE), while in flavonoids the spring leaves (107.9 mg/g DE). Using LC-ESI-MS/MS, protocatechuic acid has been identified as the most abundant compound in the spring and autumn leaves (200 and 70 μg/g DE, respectively). Amongst flavonoids, naringenin 7-O-glucoside occurred in the largest amount (20 and 10 mg/g DE in the spring and autumn leaves, respectively)...
March 28, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28341954/reversible-alopecia-with-localized-scalp-necrosis-after-accidental-embolization-of-the-parietal-artery-with-hyaluronic-acid
#13
Qing Yang, Lihong Qiu, Chenggang Yi, Ping Xue, Zhou Yu, Xianjie Ma, Yingjun Su, Shuzhong Guo
Hyaluronic acid (HA) filler injection is widely used for soft-tissue augmentation. Complications associated with HA filling are not uncommon; however, HA-induced alopecia is a rarely reported complication that could result in severe secondary psychological trauma. The etiology, clinical traits, treatment strategies, outcomes, and possible reversibility of HA-induced alopecia have not been characterized. Here, we report a case in which bilateral temple injections of 6.5 mL of HA led to persistent pain over the left scalp for several days...
March 24, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28339312/biomacromolecule-lipid-hybrid-nanoparticles-for-controlled-delivery-of-sorafenib-in-targeting-hepatocellular-carcinoma-therapy
#14
Jing Zhang, Tianqi Wang, Shengjun Mu, Livesey D Olerile, Xiaoyue Yu, Na Zhang
AIM: Hybrids composed of various materials are highly versatile for drug delivery in tumor therapy including hepatocellular carcinoma. Herein, a sorafenib (SF)-loaded biomacromolecule hyaluronic acid (HA)/lipid hybrid nanoparticles (HA/SF-cLNS) were developed for targeting drug delivery. MATERIALS & METHODS: In vitro assays determined HA/SF-cLNS release behavior, enzymatic degradation, uptake and cytotoxicity. H22-bearing liver cancer xenograft murine models were used to evaluate the biodistribution and therapeutic efficacy in vivo...
March 24, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28338415/hyaluronic-acid-hydrogel-functionalized-with-self-assembled-micelles-of-amphiphilic-pegylated-kartogenin-for-the-treatment-of-osteoarthritis
#15
Mi-Lan Kang, Se-Young Jeong, Gun-Il Im
Synthetic hyaluronic acid (HA) containing a covalently integrated drug is capable of releasing therapeutic molecules and is an attractive candidate for the intra-articular treatment of osteoarthritis (OA). Herein, self-assembled PEGylated kartogenin (PEG/KGN) micelles consisting of hydrophilic polyethylene glycol (PEG) and hydrophobic KGN, which has been shown to induce chondrogenesis in human mesenchymal stem cells, were prepared by covalent crosslinking. HA hydrogels containing PEG/KGN micelles (HA/PEG/KGN) were prepared by covalently bonding PEG chains to HA...
March 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28333326/new-high-dose-pulsed-hyaluronidase-protocol-for-hyaluronic-acid-filler-vascular-adverse-events
#16
Claudio DeLorenzi
The purpose of this article is to update the changes to the author's protocols used to manage acute filler related vascular events from those previously published in this journal. For lack of a better term, this new protocol has been called the High Dose Pulsed Hyaluronidase (HDPH) protocol for vascular embolic events with hyaluronic acid (HA) fillers. The initial protocol used involved many different modalities of treatment. The current protocol is exceedingly simple and involves solely the use of hyaluronidase in repeated high doses...
March 17, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28333176/efficacy-of-retrobulbar-hyaluronidase-injection-for-vision-loss-resulting-from-hyaluronic-acid-filler-embolization
#17
Guo-Zhang Zhu, Zhong-Sheng Sun, Wen-Xiong Liao, Bing Cai, Chun-Lin Chen, Hui-Hui Zheng, Li Zeng, Sheng-Kang Luo
Background: Vision loss is a rare but serious complication of facial hyaluronic acid (HA) filler injection, for which there is no proven rescue therapy. Retrobulbar hyaluronidase injection is advocated by many plastic surgeons as an emergency treatment, but has not been carefully assessed for its efficacy. Objectives: To evaluate the efficacy of retrobulbar hyaluronidase injection as a rescue treatment for vision loss caused by HA filler embolization. Methods: Patients with vision loss caused by HA filler embolization were treated with retrobulbar hyaluronidase injection...
January 20, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28333029/low-concentration-of-hyaluronidase-for-oocyte-denudation-can-improve-fertilization-rates-and-embryo-quality
#18
Bernardo R L de Moura, Mariana C A Gurgel, Suelen Pp Machado, Priscila A Marques, Juliana R Rolim, Marcelo C de Lima, Lister L Salgueiro
OBJECTIVE: Hyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects. METHODS: This prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/mL) used for denudation of sibling-oocytes for 22 women undergoing treatment for assisted reproduction by ICSI. A total of 192 oocytes were injected, being 104 for group I (diluted) and 88 for group II (normal)...
February 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28293868/engineering-s-equi-subsp-zooepidemicus-towards-concurrent-production-of-hyaluronic-acid-and-chondroitin-biopolymers-of-biomedical-interest
#19
Donatella Cimini, Ileana Dello Iacono, Elisabetta Carlino, Rosario Finamore, Odile F Restaino, Paola Diana, Emiliano Bedini, Chiara Schiraldi
Glycosaminoglycans, such as hyaluronic acid and chondroitin sulphate, are not only more and more required as main ingredients in cosmeceutical and nutraceutical preparations, but also as active principles in medical devices and pharmaceutical products. However, while biotechnological production of hyaluronic acid is industrially established through fermentation of Streptococcus spp. and recently Bacillus subtilis, biotechnological chondroitin is not yet on the market. A non-hemolytic and hyaluronidase negative S...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28280656/noninvasive-measurement-of-ear-cartilage-elasticity-on-the-cellular-level-a-new-method-to-provide-biomechanical-information-for-tissue-engineering
#20
Ernst Jan Bos, Koen van der Laan, Marco N Helder, Margriet G Mullender, Davide Iannuzzi, Paul P van Zuijlen
BACKGROUND: An important feature of auricular cartilage is its stiffness. To tissue engineer new cartilage, we need objective tools to provide us with the essential biomechanical information to mimic optimal conditions for chondrogenesis and extracellular matrix (ECM) development. In this study, we used an optomechanical sensor to investigate the elasticity of auricular cartilage ECM and tested whether sensitivity and measurement reproducibility of the sensor would be sufficient to accurately detect (subtle) differences in matrix compositions in healthy, diseased, or regenerated cartilage...
February 2017: Plastic and Reconstructive Surgery. Global Open
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