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hyaluronidase

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https://www.readbyqxmd.com/read/28806066/thiol-mediated-synthesis-of-hyaluronic-acid-epigallocatechin-3-o-gallate-conjugates-for-the-formation-of-injectable-hydrogels-with-free-radical-scavenging-property-and-degradation-resistance
#1
Chixuan Liu, Ki Hyun Bae, Atsushi Yamashita, Joo Eun Chung, Motoichi Kurisawa
Hyaluronic acid (HA)-based biomaterials have demonstrated only limited in vivo stability as a result of rapid degradation by hyaluronidase and reactive oxidative species. The green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG), has received considerable attention because of its powerful antioxidant and enzyme-inhibitory activities. We describe here the synthesis of HA-EGCG conjugate using a thiol-mediated reaction and its use for the preparation of a long-lasting injectable hydrogel. HA-EGCG conjugates with tunable degrees of substitution were synthesized by the nucleophilic addition reaction between EGCG quinone and thiolated HA under mild conditions...
August 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28801807/hyaluronan-mediated-mononuclear-leukocyte-binding-to-gingival-fibroblasts
#2
Daniel Hagenfeld, Nico T Mutters, Inga Harks, Raphael Koch, Ti-Sun Kim, Peter Prehm
OBJECTIVES: Binding of mononuclear leukocytes to hyaluronan cable structures is a well-known pathomechanism in several chronic inflammatory diseases, but has not yet described for chronic oral inflammations. The aim of this study was to evaluate if and how binding of mononuclear leukocytes to pathologic hyaluronan cable structures can be induced in human gingival fibroblasts. MATERIAL AND METHODS: Experiments were performed with human gingival fibroblasts and peripheral blood mononuclear cells (PBMCs) from three healthy blood donors...
August 12, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28790823/a54-peptide-mediated-functionalized-gold-nanocages-for-targeted-delivery-of-dox-as-a-combinational-photothermal-chemotherapy-for-liver-cancer
#3
Shengnan Huang, Chunming Li, Weiping Wang, Huanjie Li, Zhi Sun, Chengzhi Song, Benyi Li, Shaofeng Duan, Yurong Hu
The combination of photothermal therapy and chemotherapy (photothermal-chemotherapy) is a promising strategy for cancer therapy. Gold nanocages (AuNCs), with hollow and porous structures and unique optical properties, have become a rising star in the field of drug delivery. Here, we designed a novel targeted drug delivery system based on functionalized AuNCs and evaluated their therapeutic effects in vitro and in vivo. We then loaded doxorubicin into this promising system, designated as DHTPAuNCs consisting of hyaluronic acid-grafted and A54 peptide-targeted PEGylated AuNCs...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28789838/comparison-of-biochemical-and-cytotoxic-activities-of-extracts-obtained-from-dorsal-spines-and-caudal-fin-of-adult-and-juvenile-non-native-caribbean-lionfish-pterois-volitans-miles
#4
Aránzazu Sáenz, Natalia Ortiz, Bruno Lomonte, Alexandra Rucavado, Cecilia Díaz
Pterois volitans/miles lionfish (adult and juvenile) dorsal spines and caudal fin extracts were compared in their general composition, enzymatic activities and hemolytic and cytotoxic effects on bovine aortic endothelial cells and murine myoblasts, to distinguish between the activities present in the venom and epidermal mucus. Intradermal and intramuscular injections were also administered in mice to determine in vivo effects. This work shows that crude venom of Caribbean species of lionfish, present in dorsal spines, induces several in vitro effects including hemolysis, weak cytotoxicity, proteolytic and hyaluronidase activities, whereas in vivo, it is not hemorrhagic nor myotoxic, but causes edema, plasma extravasation and a thrombotic-associated lesion on the skin...
August 5, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28783135/enzymatic-and-pro-inflammatory-activities-of-bothrops-lanceolatus-venom-relevance-for-envenomation
#5
Marie Delafontaine, Isadora Maria Villas-Boas, Laurence Mathieu, Patrice Josset, Joël Blomet, Denise V Tambourgi
Bothrops lanceolatus, commonly named 'Fer-de-Lance', is an endemic snake of the French Caribbean Island of Martinique. Envenomations by B. lanceolatus present clinical aspects characterized by systemic thrombotic syndrome and important local inflammation, involving edema and pain but limited hemorrhage. To investigate mechanisms of venom-induced inflammation, B. lanceolatus venom was characterized, its cross-reactivity with bothropic antivenom explored, its cytotoxicity on human keratinocytes and vascular cells, and the production of cytokines and chemokines were analyzed...
August 7, 2017: Toxins
https://www.readbyqxmd.com/read/28765635/conditional-knockdown-of-hyaluronidase-2-in-articular-cartilage-stimulates-osteoarthritic-progression-in-a-mice-model
#6
Yoshitoshi Higuchi, Yoshihiro Nishida, Eiji Kozawa, Lisheng Zhuo, Eisuke Arai, Shunsuke Hamada, Daigo Morita, Kunihiro Ikuta, Koji Kimata, Takahiro Ushida, Naoki Ishiguro
The catabolism of hyaluronan in articular cartilage remains unclear. The aims of this study were to investigate the effects of hyaluronidase 2 (Hyal2) knockdown in articular cartilage on the development of osteoarthritis (OA) using genetic manipulated mice. Destabilization of the medial meniscus (DMM) model of Col2a promoter specific conditional Hyal2 knockout (Hyal (-/-) ) mice was established and examined. Age related and DMM induced alterations of articular cartilage of knee joint were evaluated with modified Mankin score and immunohistochemical staining of MMP-13, ADAMTS-5, KIAA11199, and biotinylated- hyaluronan binding protein staining in addition to histomorphometrical analyses...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28761276/efficacy-of-oral-colchicine-with-intralesional-hyaluronidase-or-triamcinolone-acetonide-in-the-grade-ii-oral-submucous-fibrosis
#7
Dipti Daga, R K Singh, U S Pal, Tikaram Gurung, Shilpi Gangwar
BACKGROUND AND OBJECTIVES: Oral submucous fibrosis (OSMF) is a potentially malignant disorder of oral mucosa affecting mainly population in South and Southeast Asia. The aim of this study is to compare the effectiveness of oral colchicine with intralesional injection of hyaluronidase or injection triamcinolone acetonide in patients with Grade II OSMF. MATERIALS AND METHODS: The study included thirty patients of clinically diagnosed Grade II OSMF. Patients were divided randomly into two groups: Group A patients were treated by administrating tablet colchicine 0...
January 2017: National Journal of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28743100/studies-on-secondary-metabolite-profiling-anti-inflammatory-potential-in-vitro-photoprotective-and-skin-aging-related-enzyme-inhibitory-activities-of-malaxis-acuminata-a-threatened-orchid-of-nutraceutical-importance
#8
Biswajit Bose, Hiranjit Choudhury, Pramod Tandon, Suman Kumaria
Malaxis acuminata D. Don., a small, terrestrial orchid, is endemic to tropical Himalayas at an altitude of 1200-2000m asl. The dried pseudobulbs are important ingredients of century old ayurvedic drug 'Ashtavarga' and a polyherbal immune-booster nutraceutical 'Chyavanprash', known to restore vigour, vitality and youthfulness. Considering tremendous medicinal importance of this threatened orchid species, a detailed study was undertaken for the first time to address its antioxidant potential, secondary metabolite contents and biological activities against skin-aging related enzymes (anti-collagenase, anti-elastase, anti-tyrosinase and xanthine oxidase) and anti-inflammatory activity (5-lipoxygenase and hyaluronidase) in different plant parts of wild and in vitro-derived plants of M...
August 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28739022/component-resolved-diagnosis-in-hymenoptera-allergy
#9
REVIEW
D Antolín-Amérigo, B Ruiz-León, E Boni, T Alfaya-Arias, M Álvarez-Mon, J Barbarroja-Escudero, D González-de-Olano, C Moreno-Aguilar, M Rodríguez-Rodríguez, M J Sánchez-González, L Sánchez-Morillas, A Vega-Castro
Component-resolved diagnosis based on the use of well-defined, properly characterised and purified natural and recombinant allergens constitutes a new approach in the diagnosis of venom allergy. Prospective readers may benefit from an up-to-date review on the allergens. The best characterised venom is that of Apis mellifera, whose main allergens are phospholipase A2 (Api m1), hyaluronidase (Api m2) and melittin (Api m4). Additionally, in recent years, new allergens of Vespula vulgaris have been identified and include phospholipase A1 (Ves v1), hyaluronidase (Ves v2) and antigen 5 (Ves v5)...
July 21, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28730807/cationic-carbon-dots-for-modification-free-detection-of-hyaluronidase-via-an-electrostatic-controlled-ratiometric-fluorescence-assay
#10
Weiqiang Yang, Jiancong Ni, Fang Luo, Wen Weng, Qiaohua Wei, Zhenyu Lin, Guonan Chen
Carbon dots (CDs) emerge as excellent fluorescent nanomaterials, but the full exploitation and application of their exceptional properties in the development of fluorescence assay are still rare. In this work, cationic carbon dots (C-CDs) covered with plenty of positive charges on the surface were synthesized through a facile ultrasonic method. Negatively charged hyaluronic acid (HA) caused the aggregation of positively charged C-CDs and neutral red (NR) along its linear chain via electrostatic adsorption, leading to a remarkable Förster resonance energy transfer (FRET) from C-CDs to NR...
July 28, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28725146/lc-esi-ms-ms-profiling-of-phenolics-from-eleutherococcus-spp-inflorescences-structure-activity-relationship-as-antioxidants-inhibitors-of-hyaluronidase-and-acetylcholinesterase
#11
Daniel Załuski, Marta Olech, Rafał Kuźniewski, Robert Verpoorte, Renata Nowak, Helena D Smolarz
Nature is a source of many plant-based molecules used as pro- or drugs. Eleutherococcus species are native to Asia and the North Russia, and are traditionally used to treat various diseases. In turn, neither secondary metabolites of the species cultivated in the West Europe nor the bioactivity is known. No differences in the phenols and flavonoids content in the inflorescences were found. The richest in polyphenols was E. giraldii (5.18 mg/g), while in flavonoids it was E. gracilistylus (1.80 mg/g). Using LC-ESI-MS/MS, protocatechuic and trans-caffeic acids have been identified as the most abundant compounds in E...
July 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28724193/saline-irrigation-for-the-management-of-skin-extravasation-injury-in-neonates
#12
REVIEW
P N Gopalakrishnan, Nitin Goel, Sujoy Banerjee
BACKGROUND: Extravasation injury, a complication commonly seen in the neonatal intensive care unit, can result in scarring with cosmetic and functional sequelae. A wide variety of treatments are available, including subcutaneous irrigation with saline (with or without hyaluronidase), liposuction, use of specific antidotes, topical applications, and normal wound care with dry or wet dressings. All such treatments aim to prevent or reduce the severity of complications. OBJECTIVES: Primary objective To compare the efficacy and safety of saline irrigation or saline irrigation with prior hyaluronidase infiltration versus no intervention or normal wound care for tissue healing in neonates with extravasation injury...
July 19, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28721300/extracellular-matrix-alterations-in-the-peyronie-s-disease
#13
Marcelo Silva Watanabe, Thérèse Rachel Theodoro, Natália Lima Coelho, Aline Mendes, Monica Luzia Pereira Leonel, Ana Maria Mader, Helena Bonciani Nader, Sidney Glina, Maria Aparecida Silva Pinhal
Peyronie's disease is characterized by fibrous plaque formation of the tunica albuginea, causing penile deformity and fertility problems. The aim of the present study was to investigate alterations in the extracellular matrix in Peyronie's disease. The study used tissues collected by surgical procedure from individuals that presented a well-established disease, while control samples were obtained by biopsies of fresh cadavers. Immunohistochemistry analysis followed by digital quantification was performed to evaluate TGF-β, heparanases and metalloproteinases (MMPs)...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28712914/ige-cross-reactivity-of-phospholipase-a2-and-hyaluronidase-of-apis-dorsata-giant-asian-honeybee-and-apis-mellifera-western-honeybee-venom-possible-use-of-a-%C3%A2-mellifera-venom-for-diagnosis-of-patients-allergic-to-a-%C3%A2-dorsata-venom
#14
Peshala Gunasekara, S M Handunnetti, Sunil Premawansa, E W R A Witharana, W M D K Dasanayake, Indra P Ratnayake, Suranjith L Seneviratne, R K S Dias, G A S Premakumara, Rajiva de Silva
Diagnostic and therapeutic reagents are unavailable for anaphylaxis arising from stings by Apis dorsata. Venom profiles and cross-reactivity of A. dorsata and Apis mellifera were compared, to ascertain whether venom of A. mellifera can be used for diagnosis in A. dorsata allergy. Both venom profiles were similar by High Performance Liquid Chromatography and SDS-PAGE. Sera of 29 of 30 (96.7%) patients with anaphylaxis to A. dorsata stings had IgE to the phospholipase-2 (PLA2) doublet (15 and 16 kDa) of A...
July 14, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28696413/genome-wide-meta-analysis-of-copy-number-variations-with-alcohol-dependence
#15
A Sulovari, Z Liu, Z Zhu, D Li
Genetic association studies and meta-analyses of alcohol dependence (AD) have reported AD-associated single nucleotide polymorphisms (SNPs). These SNPs collectively account for a small portion of estimated heritability in AD. Recent genome-wide copy number variation (CNV) studies have identified CNVs associated with AD and substance dependence, suggesting that a portion of the missing heritability is explained by CNV. We applied PennCNV and QuantiSNP CNV calling algorithms to identify consensus CNVs in five AD cohorts of European and African origins...
July 11, 2017: Pharmacogenomics Journal
https://www.readbyqxmd.com/read/28688803/hyaluronidase-like-enzymes-are-a-frequent-component-of-venoms-from-theraphosid-spiders
#16
Luis Rodríguez-Rios, Luis Fernando Díaz-Peña, Fernando Lazcano-Pérez, Roberto Arreguín-Espinosa, Alejandra Rojas-Molina, Alejandro García-Arredondo
Theraphosid spider venoms are extremely complex mixtures, composed mainly by low molecular compounds, peptides, and enzymes. The large size of these spiders and their ability to breed in captivity permits access to rather large amounts of venom and an easier venom extraction. In the present study, we conducted a comparative investigation about the content of hyaluronidase-like enzymes in the venoms from several theraphosid spiders, with a special focus on the Poecilotheria species, which are considered as underestimated theraphosids of medical importance...
July 5, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28688167/inhibition-of-hyaluronan-synthesis-attenuates-pulmonary-hypertension-associated-with-lung-fibrosis
#17
Scott D Collum, Ning-Yuan Chen, Adriana M Hernandez, Ankit Hanmandlu, Heather Sweeney, Tinne C J Mertens, Tingting Weng, Fayong Luo, Jose G Molina, Jonathan Davies, Ian P Horan, Nick W Morrell, Javier Amione-Guerra, Odeaa Al-Jabbari, Keith Youker, Wenchao Sun, Jayakumar Rajadas, Paul L Bollyky, Bindu H Akkanti, Soma Jyothula, Neeraj Sinha, Ashrith Guha, Harry Karmouty-Quintana
BACKGROUND AND PURPOSE: Group III Pulmonary hypertension (PH) is a highly lethal and widespread lung disorder that is a common complication in idiopathic pulmonary fibrosis (IPF) where it is considered to be the single most significant predictor of mortality. While increased levels of hyaluronan have been observed in IPF patients, hyaluronan-mediated vascular remodelling and the hyaluronan-mediated mechanisms promoting PH associated with IPF are not fully understood. EXPERIMENTAL APPROACH: Explanted lung tissue from patients with IPF with and without a diagnosis of PH was used to identify increased levels of hyaluronan...
July 8, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28683182/hyaluronan-collagen-hydrogels-with-sulfated-hyaluronan-for-improved-repair-of-vascularized-tissue-tune-the-binding-of-proteins-and-promote-endothelial-cell-growth
#18
Sandra Rother, Vanessa D Galiazzo, David Kilian, Karen M Fiebig, Jana Becher, Stephanie Moeller, Ute Hempel, Matthias Schnabelrauch, Johannes Waltenberger, Dieter Scharnweber, Vera Hintze
Innovative biomaterial-based concepts are required to improve wound healing of damaged vascularized tissues especially in elderly multimorbid patients. To develop functional hydrogels as 3D cellular microenvironments and as carrier or scavenging systems, e.g., for mediator proteins or proinflammatory factors, collagen fibrils are embedded into a network of photo-crosslinked acrylated hyaluronan (HA), chondroitin sulfate (CS), or sulfated HA (sHA). After lyophilization, the gels show a porous structure and an improved stability against degradation via hyaluronidase...
July 6, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28678916/erratum-for-primary-cultures-of-mouse-small-intestinal-epithelial-cells-using-the-dissociating-enzyme-type-i-collagenase-and-hyaluronidase
#19
(no author information available yet)
[This corrects the article doi: 10.1590/1414-431X20175831].
July 3, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28678057/hyaluronidase-assisted-resuscitation-in-kenya-for-severely-dehydrated-children
#20
Hijab Zubairi, Brett D Nelson, Priyanka Tulshian, Karla Fredricks, Zaid Altawil, Sarah Mireles, Fred Odongo, Thomas F Burke
BACKGROUND: Dehydration, mainly due to diarrheal illnesses, is a leading cause of childhood mortality worldwide. Intravenous (IV) therapy is the standard of care for patients who were unable to tolerate oral rehydration; however, placing IVs in fragile, dehydrated veins can be challenging. Studies in resource-rich settings comparing hyaluronidase-assisted subcutaneous rehydration with standard IV rehydration in children have demonstrated several benefits of subcutaneous rehydration, including time and success of line placement, ease of use, satisfaction, and cost-effectiveness...
July 4, 2017: Pediatric Emergency Care
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