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Jialing Dai, Shangcong Han, Fang Ju, Mei Han, Lisa Xu, Ruoyu Zhang, Yong Sun
Poor tumour penetration became a major challenge for the use of nanoparticles in anticancer therapy. To further enhance the tumour penetration efficiency, we developed a tumour-microenvironment-responsive multistage drug delivery system which was formed layer by layer via electrostatic interaction with cationic drug-loaded nanoparticles, hyaluronidase (HAase) and iRGD-modified gelatin (G-iRGD). The drug-loaded nanoparticles were formed by self-assembling mPEG-PDPA-PG and encapsulation with epirubicin (EPI)...
May 17, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Vincent Hanoux, Judith Eguida, Emmanuelle Fleurot, Jérôme Levallet, Pierre-Jacques Bonnamy
The loss of estrogen receptor α (ERα) expression in breast cancer constitutes a major hallmark of tumor progression to metastasis and is generally correlated to a strong increase in Hyaluronic Acid (HA) turnover. The aim of our study was to search for a putative link between these two major events of breast cancer progression in the estrogen receptor-positive (ER+) MCF7 breast cancer cell line. The increase in HA turnover was performed by stable overexpression of the standard CD44 (CD44S) isoform and also by treatment with exogenous Hyaluronidase (Hyal) to allow an increase in HA catabolism...
May 10, 2018: Molecular and Cellular Endocrinology
Caitlin O McAtee, Christine Booth, Christian Elowsky, Lei Zhao, Jeremy Payne, Teresa Fangman, Steve Caplan, Michael D Henry, Melanie A Simpson
The hyaluronidase Hyal1 is clinically and functionally implicated in prostate cancer progression and metastasis. Elevated Hyal1 accelerates vesicular trafficking in prostate tumor cells, thereby enhancing their metastatic potential in an autocrine manner through increased motility and proliferation. In this report, we found Hyal1 protein is a component of exosomes produced by prostate tumor cell lines overexpressing Hyal1. We investigated the role of exosomally shed Hyal1 in modulating tumor cell autonomous functions and in modifying the behavior of prostate stromal cells...
May 10, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Linawati Sutrisno, Yan Hu, Xinkun Shen, Menghuan Li, Zhong Luo, Liangliang Dai, Sixiang Wang, Julia Li Zhong, Kaiyong Cai
Infection associated with orthopedic implants is the chief cause of implant failure. An important consideration to prevent the infection at implants is to inhibit the biofilm formation for the initial 6 h. Therefore, we fabricated hyaluronidase-sensitive multilayers of chitosan (Chi)/sodium hyaluronate-lauric acid (SL) onto the surface of bone morphogenetic protein 2 (BMP2) loaded titanium nanotube (TNT) via spin-assisted layer-by-layer technique. The results of both Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (1 H NMR) confirmed the successful synthesis of SL...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Katrin-Stephanie Tücking, Roshan B Vasani, Alex A Cavallaro, Nicolas H Voelcker, Holger Schönherr, Beatriz Prieto-Simon
The development of enzyme-responsive hyaluronic acid methacrylate (HYAMA)-coated porous silicon (pSi) films and their application in electrochemical diagnostic devices for the in situ detection of the enzyme hyaluronidase (hyal), which is secreted by Staphylococcus aureus (S. aureus) bacteria, are reported. The approach relies on a HYAMA-pSi electrode made of thermally hydrocarbonized pSi (pSi-THC) that is impregnated with crosslinked HYAMA/polyethylene glycol diacrylate (PEGDA) hydrogels. The enzymatic degradation of HYAMA by bacterial hyal is monitored by differential pulse voltammetry (DPV) utilizing pSi-THC as a working electrode and ferro/ferricyanide (FF) as external redox probe...
May 11, 2018: Macromolecular Rapid Communications
Rodrigo Moraes Ferraz, Ulrika Sandkvist, Björn Lundgren
INTRODUCTION: Soft tissue fillers manufactured with hyaluronic acid (HA) dominate the filler market around the world and the fact that HA can be dissolved using hyaluronidase contributes to its popularity. Degradation of cross-linked HA products can be performed in situ and access to hyaluronidase is therefore essential for health care professionals (HCP) to perform safe filler treatments. The aim of the present study was to develop an in vivo model where hyaluronidase degradation of HA fillers can be studied in a standardized manner and then secondly, to explore the degradation of marketed HA fillers with different product characteristics...
May 1, 2018: Journal of Drugs in Dermatology: JDD
Darci R Block, Lucas J Ouverson, Craig A Wittwer, Amy K Saenger, Nikola A Baumann
BACKGROUND: Biochemical analysis of body fluids may lend insight into the pathogenesis of disease. Most commercially-available clinical laboratory methods are intended for blood-derived specimens and/or urine and laboratories must characterize the analytical performance of these methods for other specimen types such as body fluids. The aim of this work is to demonstrate one approach for characterizing analytical performance of assays for clinical analysis of body fluids. METHODS: Residual waste samples were obtained from clinically ordered testing...
May 5, 2018: Clinical Biochemistry
Marieke Rienks, Paolo Carai, Jurgen van Teeffelen, Bart Eskens, Wouter Verhesen, Bianca Hemmeryckx, Daniel M Johnson, Rick van Leeuwen, Elizabeth A Jones, Stephane Heymans, Anna-Pia Papageorgiou
Myocardial damage as a consequence of cardiotropic viruses leads to a broad variety of clinical presentations and is still a complicated condition to diagnose and treat. Whereas the extracellular matrix protein Secreted Protein Acidic and Rich in Cysteine or SPARC has been implicated in hypertensive and ischemic heart disease by modulating collagen production and cross-linking, its role in cardiac inflammation and endothelial function is yet unknown. Absence of SPARC in mice resulted in increased cardiac inflammation and mortality, and reduced cardiac systolic function upon coxsackievirus-B3 induced myocarditis...
May 3, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Ines Martin-Martin, Andrezza Campos Chagas, Anderson B Guimaraes-Costa, Laura Amo, Fabiano Oliveira, Ian N Moore, Thiago S DeSouza-Vieira, Elda E Sanchez, Montamas Suntravat, Jesus G Valenzuela, Jose M C Ribeiro, Eric Calvo
Salivary components from disease vectors help arthropods to acquire blood and have been shown to enhance pathogen transmission in different model systems. Here we show that two salivary enzymes from Lutzomyia longipalpis have a synergist effect that facilitates a more efficient blood meal intake and diffusion of other sialome components. We have previously shown that Lundep, a highly active endonuclease, enhances parasite infection and prevent blood clotting by inhibiting the intrinsic pathway of coagulation...
May 3, 2018: PLoS Pathogens
Jiajia Liu, Hongyan Kang, Xuejiao Ma, Anqiang Sun, Huiqin Luan, Xiaoyan Deng, Yubo Fan
Vascular remodeling induced by hemodynamic stimuli contributes to the pathophysiology of cardiovascular diseases. The importance of vascular cells (endothelial cells and smooth muscle cells) glycocalyx in the mechanotransduction of flow-induced shear stress at the cellular and molecular levels has been demonstrated over the past decade. However, its potential mechanotransduction role in vascular remodeling has triggered little attention. In the present study, a home-made apparatus was used to expose the rat abdominal aorta to sterile, flow or no flow, normal-pressure or high-pressure conditions for 4 days...
April 30, 2018: Hypertension
Derek H Jones
No abstract text is available yet for this article.
April 25, 2018: JAMA Dermatology
Murad Alam, Rosemara Hughart, Amelia Geisler, Kapila Paghdal, Amanda Maisel, Alexandra Weil, Dennis P West, Emir Veledar, Emily Poon
Importance: Although hyaluronidase is known to remove hyaluronic acid fillers, use of low doses has not been well studied. Objective: To assess the effectiveness and dose-related effect of small quantities of hyaluronidase to treat hyaluronic acid filler nodules. Design, Setting, and Participants: Split-arm, parallel-group, randomized clinical trial at an urban academic center. Participants were 9 healthy women. Recruitment and follow-up occurred from February 2013 to March 2014; data analysis occurred from February to July 2016...
April 25, 2018: JAMA Dermatology
Markku I Tammi, Sanna Oikari, Sanna Pasonen-Seppänen, Kirsi Rilla, Päivi Auvinen, Raija H Tammi
Hyaluronan accumulates in the stroma of several solid tumors and promotes their progression. Both enhanced synthesis and fragmentation of hyaluronan are required as a part of this inflammatory process resembling wound healing. Increased expression of the genes of hyaluronan synthases (HAS1-3) are infrequent in human tumors, while posttranslational modifications that activate the HAS enzymes, and glucose shunted to the UDP-sugar substrates HASs, can have crucial contributions to tumor hyaluronan synthesis. The pericellular hyaluronan influences virtually all cell-cell and cell-matrix interactions, controlling migration, proliferation, apoptosis, epithelial to mesenchymal transition, and stem cell functions...
April 27, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Arthur J Michalek, Laurel Kuxhaus, Daniel Jaremczuk, Nicole L Zaino
Loss of charged proteoglycans in the knee meniscus, which aid in the support of compressive loads by entraining water, is an effect of degeneration and is often associated with osteoarthritis. In healthy menisci, proteoglycan content is highest in the inner white zone and decreases towards the peripheral red zone. We hypothesized that loss of proteoglycans would reduce both osmotic swelling and compressive stiffness, spatially localized to the avascular white zone of the meniscus. This hypothesis was tested by targeted enzymatic digestion of proteoglycans using hyaluronidase in intact cervine medial menisci...
April 24, 2018: Journal of Biomechanics
Fernanda Gobbi Amorim, Tassia Rafaela Costa, Dominique Baiwir, Edwin De Pauw, Loic Quinton, Suely Vilela Sampaio
Venom composition varies across snakes from all taxonomic levels and is influenced by the snakes’ age, habitat, diet, and sexual dimorphism. The present study reports the first in-depth investigation of venom composition in male and female Bothrops moojeni ( B. moojeni ) snakes (BmooM and BmooF, respectively) through three proteomics approaches associated with functional, cytotoxic, and immunoreactivity characterization. Compared with BmooM venom, BmooF venom exhibited weaker hyaluronidase, metalloproteinase, and phospholipase activity; stronger recognition by anti-bothropic serum; 1...
April 26, 2018: Toxins
Xiuwen Guan, Jie Chen, Yingying Hu, Lin Lin, Pingjie Sun, Huayu Tian, Xuesi Chen
Tumor vaccine has been one of the research hotspots for cancer immunotherapy in recent years. By introducing tumor antigens into the body, the patient's own immune system will be specifically activated to induce effective immune responses for controlling or eliminating the malignant tumor cells. In this study, a simple nanovaccine was developed to induce antigen-specific anti-tumor immune responses. Polycationic polyethylenimine (PEI) was utilized to co-deliver the antigen ovalbumin (OVA) and the adjuvant unmethylated cytosine-phosphate-guanine (CpG) by electrostatic binding...
April 21, 2018: Biomaterials
Ran Tian, Xinyu Qiu, Pingyun Yuan, Kai Lei, Lin Wang, Yongkang Bai, Shiyu Liu, Xin Chen
Microbial infection has been considered as one of the most critical challenges in bioengineering applications especially in tissue regeneration, which engender severe threat to public health. Herein, a hydrogel performing properties of rapid self-healing, on-demand antibiosis and controlled cargo release were fabricated by a simple assembly of Fe complex as crosslinker and hyaluronic acid as gel network. This hydrogel is able to locally degrade and release Fe3+ to kill bacteria as needed, due to the hyaluronidase excreted by surrounding bacteria, resulting in efficient antibacterial activity to different types of bacteria...
April 25, 2018: ACS Applied Materials & Interfaces
Theodoros T Karalis, Paraskevi Heldin, Demitrios H Vynios, Thomas Neill, Simone Buraschi, Renato V Iozzo, Nikos K Karamanos, Spyros S Skandalis
The malignant phenotype of various cancers is linked to enhanced expression of hyaluronan, a pro-angiogenic glycosaminoglycan whose expression is suppressed by 4-methylumbelliferone (4-MU), a non-toxic oral agent used as a dietary supplement to improve health and combat prostate cancer. However, little is known about the effects of hyaluronan inhibition by 4-MU on breast cancer cells. In this study, we investigated the role of 4-MU in mammary carcinoma cells with distinct malignant phenotypes and estrogen receptor (ER) status, a major prognostic factor in the clinical management of breast cancers...
April 16, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Sabrina de Almeida Lima, Clara Guerra-Duarte, Fernanda Costal-Oliveira, Thais Melo Mendes, Luís F M Figueiredo, Daysiane Oliveira, Ricardo A Machado de Avila, Valéria Pereira Ferrer, Dilza Trevisan-Silva, Silvio S Veiga, João C Minozzo, Evanguedes Kalapothakis, Carlos Chávez-Olórtegui
Loxoscelism is the most important form of araneism in South America. The treatment of these accidents uses heterologous antivenoms obtained from immunization of production animals with crude loxoscelic venom. Due to the scarcity of this immunogen, new alternatives for its substitution in antivenom production are of medical interest. In the present work, three linear epitopes for Loxosceles astacin-like protease 1 (LALP-1) (SLGRGCTDFGTILHE, ENNTRTIGPFDYDSIMLYGAY, and KLYKCPPVNPYPGGIRPYVNV) and two for hyaluronidase (LiHYAL) (NGGIPQLGDLKAHLEKSAVDI and ILDKSATGLRIIDWEAWR) from Loxosceles intermedia spider venom were identified by SPOT-synthesis technique...
2018: Frontiers in Immunology
Ming-Kang Zhang, Xiao-Gang Wang, Jing-Yi Zhu, Miao-Deng Liu, Chu-Xin Li, Jun Feng, Xian-Zheng Zhang
This study reports a double-targeting "nanofirework" for tumor-ignited imaging to guide effective tumor-depth photothermal therapy (PTT). Typically, ≈30 nm upconversion nanoparticles (UCNP) are enveloped with a hybrid corona composed of ≈4 nm CuS tethered hyaluronic acid (CuS-HA). The HA corona provides active tumor-targeted functionality together with excellent stability and improved biocompatibility. The dimension of UCNP@CuS-HA is specifically set within the optimal size window for passive tumor-targeting effect, demonstrating significant contributions to both the in vivo prolonged circulation duration and the enhanced size-dependent tumor accumulation compared with ultrasmall CuS nanoparticles...
April 17, 2018: Small
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