keyword
MENU ▼
Read by QxMD icon Read
search

squalene

keyword
https://www.readbyqxmd.com/read/28087213/cholesterolomics-an-update
#1
William J Griffiths, Jonas Abdel-Khalik, Eylan Yutuc, Alwena H Morgan, Ian Gilmore, Thomas Hearn, Yuqin Wang
Cholesterolomics can be regarded as the identification and quantification of cholesterol, its precursors post squalene, and metabolites of cholesterol and of its precursors, in a biological sample. These molecules include 1,25-dihydroxyvitamin D3, steroid hormones and bile acids and intermediates in their respective biosynthetic pathways. In this short article we will concentrate our attention on intermediates in bile acid biosynthesis pathways, in particular oxysterols and cholestenoic acids. These molecular classes are implicated in the aetiology of a diverse array of diseases including autoimmune disease, Parkinson's disease, motor neuron disease, breast cancer, the lysosomal storage disease Niemann-Pick type C and the autosomal recessive disorder Smith-Lemli-Opitz syndrome...
January 10, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28073802/assemblage-composition-of-fungal-wood-decay-species-has-major-influence-on-how-climate-and-wood-quality-modify-decomposition
#2
Parvathy Venugopal, Kaisa Junninen, Mattias Edman, Jari Kouki
The interactions among saprotrophic fungal species, as well as their interactions with environmental factors, may have a major influence on wood decay and carbon release in ecosystems. We studied the effects that decomposer diversity (species richness and assemblage composition) have on wood decomposition when the climatic variables and substrate quality vary simultaneously. We used two temperature (16 and 21(°)C) and two humidity (70% and 90%) levels at two wood qualities (wood from managed and old-growth forests) of Pinus sylvestris In a nine-month experiment, the effects of fungal diversity were tested using four wood-decaying fungi (Antrodia xantha, Dichomitus squalens, Fomitopsis pinicola and Gloeophyllum protractum) at assemblage levels one, two and four species...
January 10, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28069586/sqle-induces-epithelial-to-mesenchymal-transition-by-regulating-of-mir-133b-in-esophageal-squamous-cell-carcinoma
#3
Yi Qin, Yi Zhang, Qinting Tang, Li Jin, Yong'an Chen
Increasing evidence suggests that microRNAs, which control gene expression at the post-transcriptional level, are aberrantly expressed in cancers and play significant roles in carcinogenesis and cancer progression. In this study, we show differential miR-133b down-expression in human esophageal squamous cell carcinoma (ESCC) cells and tissues. In addition, squalene epoxidase (SQLE), a key enzyme of cholesterol synthesis, is identified as the direct downstream target gene of miR-133b by luciferase gene reporter assay...
January 9, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28069222/ultrasound-assisted-extraction-of-bioactive-compounds-from-palm-pressed-fiber-with-high-antioxidant-and-photoprotective-activities
#4
Valéria Dal Prá, Felipe C Lunelli, Raquel Guidetti Vendruscolo, Rafael Martins, Roger Wagner, Ayres P Lazzaretti, Denise M Guimarães Freire, Maria Alexandri, Apostolis Koutinas, Marcio A Mazutti, Marcelo Barcellos da Rosa
This work is focused on the optimization of the ultrasound-assisted extraction of antioxidant compounds with photoprotective effect from palm pressed fiber. The influence of ultrasound intensity and pulse cycle was investigated by means of a central composite rotational design. The optimized condition was ultrasound intensity of 120W.cm(-2) and pulse factor of 0.4, yielding 3.24wt%. Compounds such as fatty acids, β-sitosterol, α-tocopherol, squalene, total phenolics and carotene were identified. The extract presented antioxidant activity towards synthetic (2,2-diphenyl-1-picrylhydrazyl - DPPH, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS) and biological radicals (Hydroxyl - OH), besides a sun protection factor of 15...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28035114/an-in-vitro-study-of-antileishmanial-effect-of-portulaca-oleracea-extract
#5
Elham Gharirvand Eskandari, Monir Doudi, Saeid Abedi
BACKGROUND & OBJECTIVES: Leishmaniasis is caused by protozoa of Leishmania genus and is considered as a zoonotic disease. It is a major public health problem worldwide, with high endemicity in developing countries like Iran. Various chemical drugs are used for leishmaniasis treatment, but their side-effects and the emergence of drug resistance have led to look for new effective compounds. The aim of this study was to introduce purslane (Portulaca oleracea) as a traditional and medicinal herb which might act as a valuable source for designing new pharmaceutical drug/lead against Leishmania sp...
October 2016: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/28028889/the-molecular-response-of-the-white-rot-fungus-dichomitus-squalens-to-wood-and-non-woody-biomass-as-examined-by-transcriptome-and-exoproteome-analyses
#6
Johanna Rytioja, Kristiina Hildén, Marcos Di Falco, Miaomiao Zhou, Maria Victoria Aguilar-Pontes, Outi-Maaria Sietiö, Adrian Tsang, Ronald P de Vries, Miia R Mäkelä
The ability to obtain carbon and energy is a major requirement to exist in any environment. For several ascomycete fungi (post-)genomic analyses have shown that species that occupy a large variety of habitats possess a diverse enzymatic machinery, while species with a specific habitat have a more focused enzyme repertoire that is well-adapted to the prevailing substrate. White-rot basidiomycete fungi also live in a specific habitat, as they are found exclusively in wood. In this study, we evaluated how well the enzymatic machinery of the white-rot fungus Dichomitus squalens is tailored to degrade its natural wood substrate...
December 28, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/28028887/phytochemical-analysis-of-a-cytotoxic-fraction-of-quassia-silvestris-using-lc-hr-esi-ms-n
#7
Michel Feussi Tala, Ferdinand Mouafo Talontsi, Guang-Zhi Zeng, Hippolyte Kamdem Wabo, Michael Spiteller, Ning-Hua Tan, Pierre Tane
INTRODUCTION: The genus Quassia is a promising source of secondary metabolites with biological potential including antimalarial and cytotoxic activities. Limited data are available on the phytochemistry and pharmacology of Quassia silvestris Cheek & Jongkind, a Cameroonian medicinal plant used to treat various ailments. OBJECTIVES: To carry out the bioassay-guided fractionation and LC-HR-ESI-MS analyses of the leaves extract from Q. silvestris; to purify the active fractions and isolate the major compounds using different chromatographic and spectroscopic methods...
December 28, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27982049/enhanced-stability-of-freestanding-lipid-bilayer-and-its-stability-criteria
#8
Dae-Woong Jeong, Hyunwoo Jang, Siyoung Q Choi, Myung Chul Choi
We present a new strategy to dramatically enhance the stability of freestanding lipid bilayers. We found that an addition of a water in oil emulsion stabilizer, SPAN 80 to a solvent phase guarantees nearly millimeter-scale stable freestanding lipid bilayers. The water permeability, bilayer area, contact angle, and interfacial tension were measured as a function of time and SPAN 80-to-lipid weight ratio (ΦSPAN 80) with several different solvents. Surprisingly, the SPAN 80, instead of remaining in the bilayer, was moved out of the bilayer during the bilayer formation...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27979139/fast-uplc-pda-determination-of-squalene-in-sicilian-p-d-o-pistachio-from-bronte-optimization-of-oil-extraction-method-and-analytical-characterization
#9
Andrea Salvo, Giovanna Loredana La Torre, Vita Di Stefano, Valentina Capocchiano, Valentina Mangano, Emanuele Saija, Vito Pellizzeri, Katia Erminia Casale, Giacomo Dugo
A fast reversed-phase UPLC method was developed for squalene determination in Sicilian pistachio samples that entry in the European register of the products with P.D.O. In the present study the SPE procedure was optimized for the squalene extraction prior to the UPLC/PDA analysis. The precision of the full analytical procedure was satisfactory and the mean recoveries were 92.8±0.3% and 96.6±0.1% for 25 and 50mgL(-1) level of addition, respectively. Selected chromatographic conditions allowed a very fast squalene determination; in fact it was well separated in ∼0...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27974871/determination-of-growth-phase-dependent-influences-exerted-by-prions-on-yeast-lipid-content-using-hptlc-densitometry
#10
Q Bui, J Sherma, B Fried, J K Hines
Prions of the baker's yeast Saccharomyces cerevisiae allow for the inheritance of complex traits based solely on the acquisition of cytoplasmic protein aggregates and confer distinctive phenotypes to the cells which harbor them, creating heterogeneity within an otherwise clonal cell population. These phenotypes typically arise from a loss-of-function of the prion-forming protein that is unable to perform its normal cellular function(s) while sequestered in prion amyloid aggregates, but the specific biochemical consequences of prion infection are poorly understood...
2016: Acta Chromatogr
https://www.readbyqxmd.com/read/27966972/role-of-organic-hydrocarbons-in-atmospheric-ice-formation-via-contact-freezing
#11
Kristen N Collier, Sarah D Brooks
An optical ice microscope apparatus equipped with a sealed cooling stage and a CCD camera was used to examine contact freezing events between a water droplet and ice nucleating particles (INP) containing organic hydrocarbons including octacosane, squalane, and squalene. Sample viscosities were measured with a capillary viscometer and compositions were characterized using Fourier transfer infrared spectroscopy with horizontal attenuated total reflectance and Raman microspectroscopy. All of the samples proved to be moderately efficient ice nuclei that induced freezing between -23 and -26 °C, regardless of whether the INP was solid or liquid...
December 29, 2016: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/27943451/chemical-kinetics-of-multiphase-reactions-between-ozone-and-human-skin-lipids-implications-for-indoor-air-quality-and-health-effects
#12
P S J Lakey, A Wisthaler, T Berkemeier, T Mikoviny, U Pöschl, M Shiraiwa
Ozone reacts with skin lipids such as squalene, generating an array of organic compounds, some of which can act as respiratory or skin irritants. Thus, it is important to quantify and predict the formation of these products under different conditions in indoor environments. We developed the kinetic multilayer model that explicitly resolves mass transport and chemical reactions at the skin and in the gas phase (KM-SUB-Skin). It can reproduce the concentrations of ozone and organic compounds in previous measurements and new experiments...
December 10, 2016: Indoor Air
https://www.readbyqxmd.com/read/27939849/a-squalene-synthase-protein-degradation-method-for-improved-sesquiterpene-production-in-saccharomyces-cerevisiae
#13
Bingyin Peng, Manuel R Plan, Panagiotis Chrysanthopoulos, Mark P Hodson, Lars K Nielsen, Claudia E Vickers
Sesquiterpenes are C15 isoprenoids with utility as fragrances, flavours, pharmaceuticals, and potential biofuels. Microbial fermentation is being examined as a competitive approach for bulk production of these compounds. Competition for carbon allocation between synthesis of endogenous sterols and production of the introduced sesquiterpene limits yields. Achieving balance between endogenous sterols and heterologous sesquiterpenes is therefore required to achieve economical yields. In the current study, the yeast Saccharomyces cerevisiae was used to produce the acyclic sesquiterpene alcohol, trans-nerolidol...
December 8, 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/27937037/gc-ms-analysis-and-hepatoprotective-activity-of-the-n-hexane-extract-of-acrocarpus-fraxinifolius-leaves-against-paracetamol-induced-hepatotoxicity-in-male-albino-rats
#14
Eman A Abd El-Ghffar, Heba A S El-Nashar, Omayma A Eldahshan, Abdel Nasser B Singab
CONTEXT: In Egypt, the burden of liver diseases is exceptionally high. OBJECTIVE: To investigate the components of the n-hexane extract of Acrocarpus fraxinifolius Arn. (Leguminosae) and its hepatoprotective activity against paracetamol (APAP)-induced hepatotoxicity in rats. MATERIAL AND METHODS: TRACE GC ultra gas chromatogaphic spectrometry was used for extract analysis. Thirty albino rats were divided into six groups (five rats in each)...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27931126/%C3%AE-tocopherol-%C3%AE-carotene-lutein-squalene-and-secoiridoids-in-seven-monocultivar-italian-extra-virgin-olive-oils
#15
Roberto Ambra, Fausta Natella, Sabrina Lucchetti, Valentina Forte, Gianni Pastore
Fatty acids and bioactive lipophilic and hydrophilic compounds (tocopherols, β-carotene, lutein, squalene, total polyphenols and secoiridoids) in monocultivar Italian extra-virgin olive oil (EVOO) samples produced from the Leccino cultivar and six other yet uncharacterised cultivars (Rustica, Carpinetana, Dritta, Gentile di Chieti and Intosso) were analysed, also taking into account the effect of the type of decanter used for the oil extraction. Significant differences among cultivars were found for α-tocopherol and squalene, but not for carotenoids...
December 8, 2016: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/27917337/identification-characterization-and-phylogenetic-analysis-of-antifungal-trichoderma-from-tomato-rhizosphere
#16
Shalini Rai, Prem Lal Kashyap, Sudheer Kumar, Alok Kumar Srivastava, Pramod W Ramteke
The use of Trichoderma isolates with efficient antagonistic activity represents a potentially effective and alternative disease management strategy to replace health hazardous chemical control. In this context, twenty isolates were obtained from tomato rhizosphere and evaluated by their antagonistic activity against four fungal pathogens (Fusarium oxysporum f. sp. lycopersici, Alternaria alternata, Colletotrichum gloeosporoides and Rhizoctonia solani). The production of extracellular cell wall degrading enzymes of tested isolates was also measured...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27916582/in-vitro-acaricide-activity-of-ocotea-aciphylla-nees-mez-lauraceae-extracts-and-identification-of-the-compounds-from-the-active-fractions
#17
Rodrigo Souza Conceição, Monique Marylin A de A Carneiro, Isabella Mary Alves Reis, Alexsandro Branco, Ivo Jose Curcino Vieira, Raimundo Braz-Filho, Mariana Borges Botura
The in vitro acaricide activity of hexane, ethyl acetate and ethanol extracts of Ocotea aciphylla leaves was investigated by means of an immersion tests using Rhipicephalus (Boophilus) microplus engorged females and larvae. All extracts were shown effective against the different stages of the parasite, and the ethanol extract (50mg/mL concentration) was the most active (more than 90% efficacy in both assays). The ethanolic extract was fractionated using column chromatography with silica gel as stationary phase to furnish several fractions that were tested against larvae of R...
November 28, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27905068/inhibition-of-mevalonate-pathway-and-synthesis-of-the-storage-lipids-in-human-liver-derived-and-non-liver-cell-lines-by-lippia-alba-essential-oils
#18
Sandra Montero-Villegas, Mónica Polo, Marianela Galle, Boris Rodenak-Kladniew, María Castro, Ana Ves-Losada, Rosana Crespo, Margarita García de Bravo
The essential oils (EOs) of Lippia alba, an herb extensively used as a folk medicine in Latin America, are today promoted as an effective means of eliminating problems caused by hyperlipemia. We hypothesized that L.alba EOs inhibited cholesterol and triacylglycerols synthesis and decreased the intracellular depots of those lipids (lipid droplets), mechanisms involving the induction of a hypolipidemic response. Our aim was, therefore, to evaluate the hypolipogenic capability of the EOs of four L. alba chemotypes on liver-derived (HepG2) and non-liver (A549) human cell lines and to identify the potential biochemical targets of those chemotypes, particularly within the mevalonate pathway (MP)...
November 30, 2016: Lipids
https://www.readbyqxmd.com/read/27894599/overexpression-of-the-homologous-lanosterol-synthase-gene-in-ganoderic-acid-biosynthesis-in-ganoderma-lingzhi
#19
De-Huai Zhang, Na Li, Xuya Yu, Peng Zhao, Tao Li, Jun-Wei Xu
Ganoderic acids (GAs) in Ganoderma lingzhi exhibit anticancer and antimetastatic activities. GA yields can be potentially improved by manipulating G. lingzhi through genetic engineering. In this study, a putative lanosterol synthase (LS) gene was cloned and overexpressed in G. lingzhi. Results showed that its overexpression (OE) increased the ganoderic acid (GA) content and the accumulation of lanosterol and ergosterol in a submerged G. lingzhi culture. The maximum contents of GA-O, GA-Mk, GA-T, GA-S, GA-Mf, and GA-Me in transgenic strains were 46...
February 2017: Phytochemistry
https://www.readbyqxmd.com/read/27886451/activity-of-fluorine-containing-analogues-of-wc-9-and-structurally-related-analogues-against-two-intracellular-parasites-trypanosoma-cruzi-and-toxoplasma-gondii
#20
María N Chao, Catherine Li, Melissa Storey, Bruno N Falcone, Sergio H Szajnman, Sergio M Bonesi, Roberto Docampo, Silvia N J Moreno, Juan B Rodriguez
Two obligate intracellular parasites, Trypanosoma cruzi, the agent of Chagas disease, and Toxoplasma gondii, an agent of toxoplasmosis, upregulate the mevalonate pathway of their host cells upon infection, which suggests that this host pathway could be a potential drug target. In this work, a number of compounds structurally related to WC-9 (4-phenoxyphenoxyethyl thiocyanate), a known squalene synthase inhibitor, were designed, synthesized, and evaluated for their effect on T. cruzi and T. gondii growth in tissue culture cells...
December 16, 2016: ChemMedChem
keyword
keyword
52905
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"