keyword
MENU ▼
Read by QxMD icon Read
search

squalene

keyword
https://www.readbyqxmd.com/read/28428486/antioxidative-protection-of-squalene-adjuvant-and-rabies-vaccine-with-adjuvant
#1
Anna Ondrejková, Judit Süli, Jarmila Harvanová, Róbert Ondrejka, Marián Prokeš
The authors verified the possibility of antioxidative protection of squalene adjuvant emulsions by the antioxidants α-tocopherol and β-carotene. They determined the influence of β-carotene on the stability and antigenic effectiveness of adjuvant emulsion in combination with rabies vaccine.The composition of the adjuvant emulsions or vaccines was: 2.5% squalene; 6% detergents; 0.5% antioxidant; 91% water phase. The oxidative injury after UV-irradiation was followed by the detection of the peroxide value of the emulsions...
April 19, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28424737/gc-ms-based-metabolite-profiling-antioxidant-and-antimicrobial-properties-of-different-solvent-extracts-of-malaysian-plectranthus-amboinicus-leaves
#2
Mallappa Kumara Swamy, Greetha Arumugam, Ravinder Kaur, Ali Ghasemzadeh, Mazina Mohd Yusoff, Uma Rani Sinniah
This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic (94.37 ± 1.24 mg GAE/g) and flavonoid contents (26.90 ± 1.35 mg RE/g) and exhibited the highest DPPH scavenging activity (90.13 ± 3.32%) followed by the acetone extract (80.23 ± 3.26%) at 500 μg/mL concentration. Similarly, the highest ferric ion reduction potential (849.63 ± 30.95 μM of Fe (II)/g dry weight) was exhibited by the methanol extract followed by the acetone extract (695...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28421122/potential-antitumor-activity-and-apoptosis-induction-of-glossostemon-bruguieri-root-extract-against-hepatocellular-carcinoma-cells
#3
Mona S Alwhibi, Mahmoud I M Khalil, Mohamed M Ibrahim, Gehan A El-Gaaly, Ahmed S Sultan
Glossostemon bruguieri (moghat) is used as a nutritive and demulcent drink. This study was performed to investigate the antiproliferative effects of moghat root extract (MRE) and its apoptotic mechanism in hepatocellular carcinoma (HCC) cells, HepG2 and Hep3B. MTT assay, morphological changes, apoptosis enzyme linked immunosorbent assay, caspase and apoptotic activation, flow cytometry, and immunoblot analysis were employed. The IC50 of MRE for HepG2 (910 ± 6 μg/ml) and for Hep3B (1510 ± 5 μg/ml) induced significant growth-inhibitory effects against HCC cells, with no cytotoxic effect on normal hepatocytes...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28416557/terbinafine-resistance-of-trichophyton-clinical-isolates-caused-by-specific-point-mutations-in-the-squalene-epoxidase-gene
#4
Tsuyoshi Yamada, Mari Maeda, Mohamed Mahdi Alshahni, Reiko Tanaka, Takashi Yaguchi, Olympia Bontems, Karine Salamin, Marina Fratti, Michel Monod
Terbinafine is one of the allylamine antifungal agents, whose target is squalene epoxidase (SQLE). This agent has been extensively used in the therapy of dermatophyte infections. The incidence of patients with tinea pedis or unguium tolerant to terbinafine treatment prompted us to screen terbinafine resistance of all Trichophyton clinical isolates from the laboratory of Centre Hospitalier Universitaire Vaudois collected over a three-year period, and to identify their mechanism of resistance. Among 2056 tested isolates, 17 (≈ 1%) showed reduced terbinafine susceptibility, all of which were found to harbor SQLE gene alleles with different single point mutations, leading to single amino acid substitutions at one of four positions (Leu(393), Phe(397), Phe(415) and His(440)) of the SQLE protein...
April 17, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28416486/a-squalene-based-nanomedicine-for-oral-treatment-of-colon-cancer
#5
Larissa Kotelevets, Eric Chastre, Joachim Caron, Julie Mougin, Gerard Bastian, Alain Pineau, Francine Walker, Therese Lehy, Didier Desmaele, Patrick Couvreur
Nanotechnology offers many possibilities to improve drug treatments, including with regard to drug pharmacology. The current study reports a simple approach to improve cisplatin efficacy in the treatment of colon cancer through the creation of orally administered squalenoylated nanoparticles loaded with cisplatin (SQ-CDDP NP). Cytotoxic effects of SQ-CDDP NP were assessed in human colonic cells and in mouse models of intestinal cancer. In cell culture, SQ-CDDP NP exhibited at least 10-fold greater cytotoxic potency compared to uncomplexed cisplatin, reflecting an enhancement in intracellular accumulation and DNA platination...
April 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28414174/iterative-integration-of-multiple-copy-pathway-genes-in-yarrowia-lipolytica-for-heterologous-%C3%AE-carotene-production
#6
Shuliang Gao, Yangyang Tong, Li Zhu, Mei Ge, Yian Zhang, Daijie Chen, Yu Jiang, Sheng Yang
β-Carotene is a terpenoid molecule with high hydrophobicity that is often used as an additive in foods and feed. Previous work has demonstrated the heterologous biosynthesis of β-carotene from an intrinsic high flux of acetyl-CoA in 12 steps through 11 genes in Yarrowia lipolytica. Here, an efficient biosynthetic pathway capable of producing 100-fold more β-carotene than the baseline construct was generated using strong promoters and multiple gene copies for each of the 12 steps. Using fed-batch fermentation with an optimized medium, the engineered pathway could produce 4g/L β-carotene, which was stored in lipid droplets within engineered Y...
April 13, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28403533/towards-satisfying-performance-of-an-o-w-cosmetic-emulsion-screening-of-reformulation-factors-on-textural-and-rheological-properties-using-general-experimental-design
#7
M Filipovic, M Lukic, S Djordjevic, V Krstonosic, I Pantelic, G Vuleta, S Savic
OBJECTIVE: Consumers' demand for improved products' performance, alongside with the obligation of meeting the safety and efficacy goals, presents a key reason for the reformulation, as well as a challenging task for formulators. Any change of the formulation, whether it is wanted - in order to innovate the product (new actives and raw materials) or necessary - due to, e.g., legislative changes (restriction of ingredients), ingredients market unavailability, new manufacturing equipment, etc...
April 12, 2017: International Journal of Cosmetic Science
https://www.readbyqxmd.com/read/28375126/tyrosinase-inhibition-anti-acetylcholinesterase-and-antimicrobial-activities-of-the-phytochemicals-from-gynotroches-axillaris-blume
#8
Salam Ahmed Abed, Hasnah Mohd Sirat, Muhammad Taher
The leaves of Gynotroches axillaris were chemically and biologically studied. Sequential extraction of the leaves using petroleum ether, chloroform, and methanol afforded three extracts. Purification of pet. ether extract yielded, squalene and β-amyrin palmitate as the major compounds, together with palmitic acid and myristic acid as the minor components. The methanol extract yielded two flavonoids, quercitrin and epicatechin. The isolated compounds were characterized by MS, IR and NMR (1D and 2D). Anti-acetyl cholinesterase screening using TLC bio-autography assay showed that palmitic acid and myristic acid were the strongest inhibition with detection limit 1...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28370710/probing-skin-physiology-through-the-volatile-footprint-discriminating-volatile-emissions-before-and-after-acute-barrier-disruption
#9
Emer Duffy, Matthew R Jacobs, Brian Kirby, Aoife Morrin
Volatile organic compounds emitted by human skin were sampled before and after acute barrier disruption of the volar forearm to investigate the significance of this approach to skin physiology research. A small wearable housing integrating a solid phase micro-extraction fibre permitting rapid enclosed headspace sampling of human skin volatiles is presented, enabling non-invasive sample collection in 15 minutes, in a comfortable wearable format. Gas chromatography-mass spectrometry was utilised to separate and identify the volatile metabolites...
March 31, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28365988/improvement-of-squalene-production-from-co2-in-synechococcus-elongatus-pcc-7942-by-metabolic-engineering-and-scalable-production-in-a-photobioreactor
#10
Sun Young Choi, Jin-Young Wang, Ho Seok Kwak, Sun-Mi Lee, Youngsoon Um, Yunje Kim, Sang Jun Sim, Jong-Il Choi, Han Min Woo
The push-and-pull strategy for metabolic engineering was successfully demonstrated in Synechococcus elongatus PCC 7942, a model photosynthetic bacterium, to produce squalene from CO2. Squalene synthase (SQS) was fused to either a key enzyme (farnesyl diphosphate synthase) of the methylerythritol phosphate pathway or the β-subunit of phycocyanin (CpcB1). Engineered cyanobacteria with expression of a fusion CpcB1-SQS protein showed a squalene production level (7.16 ± 0.05 mg/L/OD730) that was increased by 1...
April 3, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28360334/a-genomic-dna-reporter-screen-identifies-squalene-synthase-inhibitors-which-act-cooperatively-with-statins-to-upregulate-the-low-density-lipoprotein-receptor
#11
Alastair G Kerr, Lawrence C S Tam, Ashley B Hale, Milena Cioroch, Gillian Douglas, Sarina Agkatsev, Olivia Hibbit, Joseph Mason, James Holt-Martyn, Carole J R Bataille, Graham M Wynne, Keith M Channon, Angela J Russell, Richard Wade-Martins
Hypercholesterolaemia remains one of the leading risk factors for the development of cardiovascular disease. Many large double-blind studies have demonstrated that lowering LDL-cholesterol using a statin can reduce the risk of having a cardiovascular event by ~30%. However, despite the success of statins, some patient populations are unable to lower their LDL-cholesterol to meet the targeted lipid levels, due to compliance or potency issues. This is especially true for heterozygous familial hypercholesterolaemia (heFH) patients who may require additional upregulation of the Low-Density Lipoprotein Receptor (LDLR) to reduce LDL-cholesterol levels below those achievable with maximal dosing of statins...
March 30, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28353148/molecular-and-biochemical-characterization-of-squalene-synthase-from-siraitia-grosvenorii
#12
Heling Su, Yongming Liu, Yalun Xiao, Yanlian Tan, Yunyan Gu, Bin Liang, Hongli Huang, Yaosheng Wu
OBJECTIVES: To clone and characterize the squalene synthase from Siraitia grosvenorii (SgSQS). RESULTS: The gene encoding SgSQS was cloned. SgSQS has 417 amino acid residues with an pI of 7.3. There are 32 phosphorylation sites in its sequence: S(48) as well as S(196) play important roles in regulation of enzyme activity. The enzyme is a monomeric protein with a cave-like active center formed by α helixes and has two transmembrane domains at its C-terminus. SgSQS mRNA expression in stem and root were about twice as much as that in leaf and peel...
March 28, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28349256/virus-induced-gene-silencing-of-the-two-squalene-synthase-isoforms-of-apple-tree-malus%C3%A2-%C3%A3-%C3%A2-domestica-l-negatively-impacts-phytosterol-biosynthesis-plastid-pigmentation-and-leaf-growth
#13
Sandra M Navarro Gallón, Carolina Elejalde-Palmett, Dimitri Daudu, Franziska Liesecke, Frédéric Jullien, Nicolas Papon, Thomas Dugé de Bernonville, Vincent Courdavault, Arnaud Lanoue, Audrey Oudin, Gaëlle Glévarec, Olivier Pichon, Marc Clastre, Benoit St-Pierre, Lucia Atehortùa, Nobuyuki Yoshikawa, Nathalie Giglioli-Guivarc'h, Sébastien Besseau
The use of a VIGS approach to silence the newly characterized apple tree SQS isoforms points out the biological function of phytosterols in plastid pigmentation and leaf development. Triterpenoids are beneficial health compounds highly accumulated in apple; however, their metabolic regulation is poorly understood. Squalene synthase (SQS) is a key branch point enzyme involved in both phytosterol and triterpene biosynthesis. In this study, two SQS isoforms were identified in apple tree genome. Both isoforms are located at the endoplasmic reticulum surface and were demonstrated to be functional SQS enzymes using an in vitro activity assay...
March 27, 2017: Planta
https://www.readbyqxmd.com/read/28342963/new-insights-into-cellular-cholesterol-acquisition-promoter-analysis-of-human-hmgcr-and-sqle-two-key-control-enzymes-in-cholesterol-synthesis
#14
Vicky Howe, Laura J Sharpe, Anika V Prabhu, Andrew J Brown
BACKGROUND: The two control points of cholesterol synthesis, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) and squalene monooxygenase (SQLE) are known targets of the transcription factor sterol-regulatory element binding protein-2 (SREBP-2). Yet the location of the sterol-regulatory elements (SREs) and cofactor binding sites, nuclear factor-Y (NF-Y) and specificity protein 1 (Sp1), have not been satisfactorily mapped in the human SQLE promoter, or at all in the human HMGCR promoter...
March 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28342753/evaluation-of-chitosan-as-a-wall-material-for-microencapsulation-of-squalene-by-spray-drying-characterization-and-oxidative-stability-studies
#15
Lekshmi R G Kumar, N S Chatterjee, C S Tejpal, K V Vishnu, K K Anas, K K Asha, R Anandan, Suseela Mathew
The present study was aimed at investigating the efficacy of chitosan as a wall material for microencapsulation of squalene by spray drying for functional food applications. Based on different core to wall material ratio (1:1, 0.5:1 and 0.3:1 on w/w basis), emulsions were prepared and evaluated in terms of emulsion stability, particle size, zeta potential, polydispersity Index (PDI), rheology and microstructure. The optimized emulsion combination was spray dried and characterized, physically and chemically...
March 22, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28340748/mitigation-of-solvent-interference-using-a-short-packed-column-prior-to-ion-mobility-spectrometry
#16
Mohammad T Jafari, Mohammad Saraji, Mehdi Mossaddegh
This paper introduces a novel approach to overcome the solvent interference in corona discharge-ion mobility spectrometry (CD-IMS) based on the time-resolved signals of the solvent and the analyte. To that end, a short Teflon tube was filled with a low amount of squalene or OV-1, which was prepared and located between the injection port and the entrance of the CD-IMS cell. Through this procedure, a sufficient delay (~5s) was obtained between the introduction of the solvent and the analyte into the reaction region of IMS...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28336949/induction-of-apoptosis-and-ganoderic-acid-biosynthesis-by-camp-signaling-in-ganoderma-lucidum
#17
Bang-Jau You, Ni Tien, Miin-Huey Lee, Bo-Ying Bao, Yih-Shyuan Wu, Tsung-Chi Hu, Hong-Zin Lee
Apoptosis is an essential physiological process that controls many important biological functions. However, apoptosis signaling in relation to secondary metabolite biosynthesis in plants and fungi remains a mystery. The fungus Ganoderma lucidum is a popular herbal medicine worldwide, but the biosynthetic regulation of its active ingredients (ganoderic acids, GAs) is poorly understood. We investigated the role of 3',5'-cyclic adenosine monophosphate (cAMP) signaling in fungal apoptosis and GA biosynthesis in G...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28322148/effects-of-exogenous-salicylic-acid-on-ganoderic-acid-biosynthesis-and-the-expression-of-key-genes-in-the-ganoderic-acid-biosynthesis-pathway-in-the-lingzhi-or-reishi-medicinal-mushroom-ganoderma-lucidum-agaricomycetes
#18
Peng-Fei Cao, Chen-Gao Wu, Zhi-Hao Dang, Liang Shi, Ai-Liang Jiang, Ang Ren, Ming-Wen Zhao
We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.04% compared with the control group. When the fungi were treated with 200 μmol/L SA at different times, the GA content was improved 10.21% to 35.24% compared with the control group. By choosing the optimum point based on response surface methodology, the GA content could be increased up to 229...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28317728/direct-study-of-minor-extra-virgin-olive-oil-components-without-any-sample-modification-1-h-nmr-multisupression-experiment-a-powerful-tool
#19
Ainhoa Ruiz-Aracama, Encarnación Goicoechea, María D Guillén
Proton Nuclear Magnetic Resonance ((1)H NMR) was employed to study monovarietal commercial Spanish extra-virgin olive oils (EVOO) (Arbequina, Arroniz, Cornicabra, Hojiblanca and Picual). Each sample was analyzed by a standard pulse and by an experiment suppressing the main lipid signals, enabling the detection of signals of minor components. The aim was to determine the possibilities of both (1)H NMR approaches to characterize EVOO composition, focusing on acyl groups, squalene, sterols, triterpene acids/esters, fatty alcohols, wax esters and phenols (lignans, tyrosol, hydroxytyrosol, oleocanthal, oleacein, oleokoronal, oleomissional, ligstrodials and oleuropeindials), and to determine hydrolysis and oxidation levels...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28315676/the-major-histocompatibility-complex-class-iii-haplotype-ltab-ncr3-regulates-adjuvant-induced-but-not-antigen-induced-autoimmunity
#20
Anthony C Y Yau, Jonatan Tuncel, Rikard Holmdahl
Rheumatoid arthritis is a complex disease associated with >100 risk loci, with the strongest association from the major histocompatibility complex (MHC) region. Here, we analyzed a new genetic association in the MHC class-III region (MHC-III) using adjuvant- and antigen-induced arthritis models. In addition, we used models for multiple sclerosis for comparison and dissected the MHC-III-mediated mechanisms of importance for antibody and T-cell responses to antigens. With the use of a panel of MHC-III recombinant inbred strains, we found that the 33-kb Ltab-Ncr3 haplotype in MHC-III was linked to the induction of arthritis with incomplete Freund's adjuvant, with similar effects in arthritis induced by several oil adjuvants (hexadecane, heptadecane, squalene, arlacel)...
March 15, 2017: American Journal of Pathology
keyword
keyword
52905
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"