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Oleic acid hepg2

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https://www.readbyqxmd.com/read/28165858/plga-nanoparticles-for-the-oral-delivery-of-nuciferine-preparation-physicochemical-characterization-and-in-vitro-in-vivo-studies
#1
Ying Liu, Xin Wu, Yushuai Mi, Bimeng Zhang, Shengying Gu, Gaolin Liu, Xiaoyu Li
This article reports a promising approach to enhance the oral delivery of nuciferine (NUC), improve its aqueous solubility and bioavailability, and allow its controlled release as well as inhibiting lipid accumulation. NUC-loaded poly lactic-co-glycolic acid nanoparticles (NUC-PLGA-NPs) were prepared according to a solid/oil/water (s/o/w) emulsion technique due to the water-insolubility of NUC. PLGA exhibited excellent loading capacity for NUC with adjustable dosing ratios. The drug loading and encapsulation efficiency of optimized formulation were 8...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28125673/reversible-nuclear-lipid-droplet-morphology-induced-by-oleic-acid-a-link-to-cellular-lipid-metabolism
#2
Lucía C Lagrutta, Sandra Montero-Villegas, Juan P Layerenza, Martín S Sisti, Margarita M García de Bravo, Ana Ves-Losada
Neutral lipids-involved in many cellular processes-are stored as lipid droplets (LD), those mainly cytosolic (cLD) along with a small nuclear population (nLD). nLD could be involved in nuclear-lipid homeostasis serving as an endonuclear buffering system that would provide or incorporate lipids and proteins involved in signalling pathways as transcription factors and as enzymes of lipid metabolism and nuclear processes. Our aim was to determine if nLD constituted a dynamic domain. Oleic-acid (OA) added to rat hepatocytes or HepG2 cells in culture produced cellular-phenotypic LD modifications: increases in TAG, CE, C, and PL content and in cLD and nLD numbers and sizes...
2017: PloS One
https://www.readbyqxmd.com/read/28119530/decreased-lipid-metabolism-but-increased-fa-biosynthesis-are-coupled-with-changes-in-liver-micrornas-in-obese-subjects-with-nafld
#3
J Latorre, J M Moreno-Navarrete, J M Mercader, M Sabater, Ò Rovira, J Gironès, W Ricart, J M Fernández-Real, F J Ortega
BACKGROUND/OBJECTIVE: Many controversies regarding the association of liver miRNAs with obesity and nonalcoholic fatty liver diseases (NAFLD) call for additional validations. This study sought to investigate variations in genes and hepatic miRNAs in a sample of obese patients with or without NAFLD and human hepatocytes. SUBJECTS/METHODS: Sixty non-consecutive obese women following bariatric surgery were recruited. Subjects were classified as NAFLD (n=17), borderline (n=24), and controls (n=19) with normal enzymatic profile, liver histology, and ultrasound assessments...
January 25, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28067838/effect-of-phenolic-compounds-from-elderflowers-on-glucose-and-fatty-acid-uptake-in-human-myotubes-and-hepg2-cells
#4
Giang Thanh Thi Ho, Eili Tranheim Kase, Helle Wangensteen, Hilde Barsett
Type 2 diabetes (T2D) is manifested by progressive metabolic impairments in tissues such as skeletal muscle and liver, and these tissues become less responsive to insulin, leading to hyperglycemia. In the present study, stimulation of glucose and oleic acid uptake by elderflower extracts, constituents and metabolites were tested in vitro using the HepG2 hepatocellular liver carcinoma cell line and human skeletal muscle cells. Among the crude extracts, the 96% EtOH extract showed the highest increase in glucose and oleic acid uptake in human skeletal muscle cells and HepG2-cells...
January 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27996362/the-effects-of-di-2-ethyl-hexyl-phthalate-dehp-on-cellular-lipid-accumulation-in-hepg2-cells-and-its-potential-mechanisms-in-the-molecular-level
#5
Wang Zhang, Xin-Yue Shen, Wen-Wen Zhang, Hao Chen, Wei-Ping Xu, Wei Wei
Diethylhexyl phthalate (DEHP) is suspected to be an inevitable factor related to metabolic disease. Our previous study demonstrated that excess DEHP could exacerbate Non Alcoholic Fatty Liver Disease(NAFLD) in SD rats. Addressing the terra incognita in DEHP-induced metabolic dysfunction, this study used HepG2 cells to investigate the potential mechanisms involved in DEHP-induced toxicity in vitro. The cells were established lipid overload model with oleic acid and BSA, then exposed to different concentrations (5, 10, 25, 50, 100 μmol/l DEHP) of DEHP for further analysis...
December 20, 2016: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/27912736/citrus-junos-tanaka-peel-ameliorates-hepatic-lipid-accumulation-in-hepg2-cells-and-in-mice-fed-a-high-cholesterol-diet
#6
Eun Ju Shin, Jae Ho Park, Mi Jeong Sung, Min-Yu Chung, Jin-Taek Hwang
BACKGROUND: Citrus junos Tanaka (yuja), a yellow-coloured citrus fruit has traditionally been consumed in Korea, Japan, and China and has been found effective in preventing certain diseases. However, the inhibitory effect of yuja on hepatic lipid accumulation has not been clearly elucidated thus far. METHODS: The inhibitory effect of yuja on hepatic lipid accumulation was investigated in both cell culture and mouse models. We investigated the inhibitory effect of ethanol extract of yuja peel (YE) using HepG2 cells...
December 3, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27845265/the-effects-of-herbal-composition-gambigyeongsinhwan-4-on-hepatic-steatosis-and-inflammation-in-otsuka-long-evans-tokushima-fatty-rats-and-hepg2-cells
#7
Seolah Yoon, Jeongjun Kim, Hyunghee Lee, Haerim Lee, Jonghoon Lim, Heejeong Yang, Soon Shik Shin, Michung Yoon
ETHNOPHARMACOLOGICAL RELEVANCE: Hepatic steatosis has risen rapidly in parallel with a dramatic increase in obesity. The aim of this study was to determine whether the herbal composition Gambigyeongsinhwan (4) (GGH(4)), composed of Curcuma longa L. (Zingiberaceae), Alnus japonica (Thunb.) Steud. (Betulaceae), and the fermented traditional Korean medicine Massa Medicata Fermentata, regulates hepatic steatosis and inflammation. MATERIALS AND METHODS: The effects of GGH(4) on hepatic steatosis and inflammation in Otsuka Long-Evans Tokushima fatty (OLETF) rats and HepG2 cells were examined using Oil red O, hematoxylin and eosin, and toluidine blue staining, immunohistochemistry, quantitative real-time polymerase chain reaction, and peroxisome proliferator-activated receptor α (PPARα) transactivation assay...
January 4, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27627919/resveratrol-ameliorates-alcoholic-fatty-liver-by-inducing-autophagy
#8
Liying Tang, Fengli Yang, Zhirui Fang, Chengmu Hu
Alcoholic fatty liver (AFL) is early stage of alcoholic liver disease, which can progress to steatohepatitis, fibrosis, and cirrhosis if alcohol consumption is continued. The pathogenesis of AFL is associated with excessive lipid accumulation in hepatocytes. Resveratrol (RES), a dietary polyphenol found in red wines and grapes, has been shown to have a hepatoprotective effect. Autophagy is a crucial physiological process in cellular catabolism that involves the regulation of lipid droplets. Autophagy maintains a balance between protein synthesis, degradation and self-recycling...
2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27565442/calcium-sensing-receptor-effects-in-adipocytes-and-liver-cells-implications-for-an-adipose-hepatic-crosstalk
#9
Pia Villarroel, Pamela Mattar, Amanda D'Espessailles, Marco Arrese, Andrea Arreguin, Cecilia Fuentes, Marcela Reyes, Mariana Cifuentes
The calcium sensing receptor (CaSR) is expressed in human adipose cells, and its activation may associate with adipose tissue (AT) dysfunction. We evaluated whether CaSR stimulation influences adipocyte triglyceride (TG) and fatty acid binding protein 4 (aP2) content, and hepatocyte TGs and proinflammatory cytokine expression. The effect of the calcimimetic cinacalcet on TGs (fluorimetry), lipogenic genes (qPCR) and aP2 (immunoblot) was evaluated in LS14 adipocytes or AT. In the human HepG2 hepatic cell line, we assessed CaSR expression and cinacalcet effect on TGs and lipogenic and proinflammatory genes...
October 1, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27490779/swertiamarin-ameliorates-oleic-acid-induced-lipid-accumulation-and-oxidative-stress-by-attenuating-gluconeogenesis-and-lipogenesis-in-hepatic-steatosis
#10
Tushar P Patel, Komal Rawal, Sanket Soni, Sarita Gupta
Swertiamarin, a bitter secoiridoid glycoside, is an antidiabetic drug with lipid lowering activity meliorates insulin resistance in Type 2 Diabetes condition. Therefore, the study was designed to explore the antioxidant and hypolipidemic activity of swertiamarin in ameliorating NAFLD caused due to hepatic lipid accumulation, inflammation and insulin resistance. Steatosis was induced in HepG2 cells by supplementing 1mM oleic acid (OA) for 24h which was marked by significant accumulation of lipid droplets. This was determined by Oil Red O (ORO) staining and triglyceride accumulation...
October 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27456850/anti-lipoapoptotic-effects-of-alisma-orientalis-extract-on-non-esterified-fatty-acid-induced-hepg2-cells
#11
Hyeon-Soo Jeong, Young-Hwan Cho, Kang-Hoon Kim, Yumi Kim, Ki-Suk Kim, Yun-Cheol Na, Jiyoung Park, In-Seung Lee, Jang-Hoon Lee, Hyeung-Jin Jang
BACKGROUND: Liver steatosis was caused by lipid accumulation in the liver. Alisma orientale (AO) is recognized as a promising candidate with therapeutic efficacy for the treatment of nonalcoholic fatty liver disease (NAFLD). HepG2 hepatocyte cell line is commonly used for liver disease cell model. METHOD: The HepG2 cells were cultured with the NEFAs mixture (oleic and palmitic acids, 2:1 ratio) for 24 h to induce hepatic steatosis. Then different doses of Alisma orientale extract (AOE) was treated to HepG2 for 24 h...
July 25, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27446914/in-vitro-modulation-of-the-lps-induced-proinflammatory-profile-of-hepatocytes-and-macrophages-approaches-for-intervention-in-obesity
#12
Ramiar K Kheder, James Hobkirk, Cordula M Stover
Low grade endotoxemia is a feature of obesity which is linked to development of steatohepatitis in non-alcoholic fatty liver disease. In this study, macrophages (J774) and hepatocytes (HepG2) were stimulated with lipopolysaccharide (LPS) from E. coli 0111: B4 and analyzed for modulation of this response when preconditioned or stimulated subsequent to LPS, with different doses of Vitamin D3 or docosahexaenoic acid (DHA) over a time period of 1 and 5 days. Pro-inflammatory TNFα and pro-fibrotic TGFβ released into the supernatants were measured by ELISA; qPCR was performed for Srebp-1c and PPARα mRNA (genes for products involved in fatty acid synthesis and catabolism, respectively)...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27438262/investigating-nonalcoholic-fatty-liver-disease-in-a-liver-on-a-chip-microfluidic-device
#13
Manuele Gori, Maria Chiara Simonelli, Sara Maria Giannitelli, Luca Businaro, Marcella Trombetta, Alberto Rainer
BACKGROUND AND AIM: Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease worldwide, ranging from simple steatosis to nonalcoholic steatohepatitis, which may progress to cirrhosis, eventually leading to hepatocellular carcinoma (HCC). HCC ranks as the third highest cause of cancer-related death globally, requiring an early diagnosis of NAFLD as a potential risk factor. However, the molecular mechanisms underlying NAFLD are still under investigation. So far, many in vitro studies on NAFLD have been hampered by the limitations of 2D culture systems, in which cells rapidly lose tissue-specific functions...
2016: PloS One
https://www.readbyqxmd.com/read/27313828/s-propargyl-cysteine-exerts-a-novel-protective-effect-on-methionine-and-choline-deficient-diet-induced-fatty-liver-via-akt-nrf2-ho-1-pathway
#14
Wenwen Li, Fenfen Ma, Laiyin Zhang, Yong Huang, Xinghui Li, Aijie Zhang, Cuilan Hou, Yichun Zhu, YiZhun Zhu
This study investigated the antioxidative effect of S-propargyl-cysteine (SPRC) on nonalcoholic fatty liver (NAFLD) by treating mice fed a methionine and choline deficient (MCD) diet with SPRC for four weeks. We found that SPRC significantly reduced hepatic reactive oxygen species (ROS) and methane dicarboxylic aldehyde (MDA) levels. Moreover, SPRC also increased the superoxide dismutase (SOD) activity. By Western blot, we found that this protective effect of SPRC was importantly attributed to the regulated hepatic antioxidant-related proteins, including protein kinase B (Akt), heme oxygenase-1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf2), and cystathionine γ-lyase (CSE, an enzyme that synthesizes hydrogen sulfide)...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27275117/the-effect-of-chitooligosaccharides-on-oleic-acid-induced-lipid-accumulation-in-hepg2-cells
#15
Peiqiu Cao, Guidong Huang, Qingyun Yang, Jiao Guo, Zhengquan Su
This experiment aimed to evaluate the capacities of two types of chitooligosaccharides (COS) with different molecular weights for the ability to eliminate lipid accumulation in hepatocytes. We have established a lipid accumulation model in HepG2 cells for these studies in vitro, which was established by induction with oleic acid. The capacity of COS to eliminate lipid accumulation was evaluated using three metrics: the thiazolyl blue dye absorbance (MTT value), the morphology of intracellular lipid droplets and the triglyceride level (TG)...
May 2016: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/27265384/dihydromyricetin-ameliorates-oleic-acid-induced-lipid-accumulation-in-l02-and-hepg2-cells-by-inhibiting-lipogenesis-and-oxidative-stress
#16
Caifeng Xie, Zhen Chen, Chengfu Zhang, Xin Xu, Jiangbo Jin, Weihua Zhan, Tianyu Han, Jianbin Wang
AIMS: Dihydromyricetin (DMY), a flavonoid component isolated from Ampelopsis grossedentata, was recently reported to ameliorate nonalcoholic fatty liver disease (NAFLD) in patients. However, the underlying mechanisms of this action remain unknown. Here, we evaluate the effect of DMY on an in vitro model of NAFLD and investigate the signal transduction pathways underlying DMY treatment. MAIN METHODS: Oleic acid (OA) induced hepatic steatosis was established in L02 and HepG2 cells as in vitro model of NAFLD...
July 15, 2016: Life Sciences
https://www.readbyqxmd.com/read/27237960/lipid-droplets-affect-elimination-of-porphyromonas-gingivalis-in-hepg2-cells-by-altering-the-autophagy-lysosome-system
#17
Yumi Zaitsu, Mayumi Iwatake, Keiko Sato, Takayuki Tsukuba
Recent studies have shown that infection with Porphyromonas gingivalis, a major periodontal pathogen, hastens the progression of non-alcoholic fatty liver disease (NAFLD). However, the intracellular fate of P. gingivalis in hepatocytes remains unknown. Here, using oleic-acid-induced HepG2 cells as an in vitro model for NAFLD, we found that lipid droplets increased the existence of P. gingivalis in the cells at an early phase of infection. Confocal microscopic analysis revealed that lipid droplets affected the formation of autolysosomes in infected cells...
September 2016: Microbes and Infection
https://www.readbyqxmd.com/read/27176782/treatment-with-estrogen-protects-against-ovariectomy-induced-hepatic-steatosis-by-increasing-aqp7-expression
#18
Xiaohua Fu, Lili Xing, Weihai Xu, Jing Shu
Recent evidence has suggested that the marked decrease in ovarian secretion of estrogens in postmenopausal women may be associated with the development of non-alcoholic fatty liver disease. The present study aimed to elucidate the mechanisms by which low levels of estrogen induce fatty liver disease using an ovariectomized (OVX) mouse model and an in vitro cell model. A total of 24 female C57/BL6 mice were divided into four groups: Sham operation, sham operation plus subcutaneous implantation of tamoxifen (TAM), bilateral OVX, and OVX plus subcutaneous implantation of 17β-estradiol (E2)...
July 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27123455/the-compound-of-mangiferin-berberine-salt-has-potent-activities-in-modulating-lipid-and-glucose-metabolisms-in-hepg2-cells
#19
Can Wang, Jian-Dong Jiang, Wei Wu, Wei-Jia Kong
The mangiferin-berberine (MB) salt was synthesized by ionic bonding of mangiferin (M) and berberine (B) at an equal molecular ratio. This study aimed to investigate the activities of MB salt in modulating lipid and glucose metabolisms in HepG2 cells. After 24 h treatment of the studying compounds, cellular AMP-activated protein kinase α (AMPKα)/acetyl-CoA carboxylase (ACC) protein levels and carnitine palmitoyltransferase (CPT) 1 activities, intracellular lipid contents, mRNA expression levels of target genes, glucose consumption, and glucose production amounts were determined...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27084371/pinolenic-acid-downregulates-lipid-anabolic-pathway-in-hepg2-cells
#20
Ah Ron Lee, Sung Nim Han
Pine nut oil (PNO) was reported to reduce lipid accumulation in the liver. However, the specific effect of pinolenic acid (18:3, all-cis-Δ5,9,12), a unique component of PNO, on lipid metabolism has not been studied. We hypothesized that pinolenic acid downregulates the lipid anabolic pathway in HepG2 cells. HepG2 cells were incubated in serum-free medium supplemented with 50 μM bovine serum albumin (BSA), palmitic acid, oleic acid, γ-linolenic acid, pinolenic acid, eicosapentaenoic acid (EPA), or α-linolenic acid for 24 h...
July 2016: Lipids
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