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Oleic acid hepg2

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https://www.readbyqxmd.com/read/27912736/citrus-junos-tanaka-peel-ameliorates-hepatic-lipid-accumulation-in-hepg2-cells-and-in-mice-fed-a-high-cholesterol-diet
#1
Eun Ju Shin, Jae Ho Park, Mi Jeong Sung, Min-Yu Chung, Jin-Taek Hwang
BACKGROUND: Citrus junos Tanaka (yuja), a yellow-coloured citrus fruit has traditionally been consumed in Korea, Japan, and China and has been found effective in preventing certain diseases. However, the inhibitory effect of yuja on hepatic lipid accumulation has not been clearly elucidated thus far. METHODS: The inhibitory effect of yuja on hepatic lipid accumulation was investigated in both cell culture and mouse models. We investigated the inhibitory effect of ethanol extract of yuja peel (YE) using HepG2 cells...
December 3, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27845265/the-effects-of-herbal-composition-gambigyeongsinhwan-4-on-hepatic-steatosis-and-inflammation-in-otsuka-long-evans-tokushima-fatty-rats-and-hepg2-cells
#2
Seolah Yoon, Jeongjun Kim, Hyunghee Lee, Haerim Lee, Jonghoon Lim, Heejeong Yang, Soon Shik Shin, Michung Yoon
ETHNOPHARMACOLOGICAL RELEVANCE: Hepatic steatosis has risen rapidly in parallel with a dramatic increase in obesity. The aim of this study was to determine whether the herbal composition Gambigyeongsinhwan (4) (GGH(4)), composed of Curcuma longa L. (Zingiberaceae), Alnus japonica (Thunb.) Steud. (Betulaceae), and the fermented traditional Korean medicine Massa Medicata Fermentata, regulates hepatic steatosis and inflammation. MATERIALS AND METHODS: The effects of GGH(4) on hepatic steatosis and inflammation in Otsuka Long-Evans Tokushima fatty (OLETF) rats and HepG2 cells were examined using Oil red O, hematoxylin and eosin, and toluidine blue staining, immunohistochemistry, quantitative real-time polymerase chain reaction, and peroxisome proliferator-activated receptor α (PPARα) transactivation assay...
November 11, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27627919/resveratrol-ameliorates-alcoholic-fatty-liver-by-inducing-autophagy
#3
Liying Tang, Fengli Yang, Zhirui Fang, Chengmu Hu
Alcoholic fatty liver (AFL) is early stage of alcoholic liver disease, which can progress to steatohepatitis, fibrosis, and cirrhosis if alcohol consumption is continued. The pathogenesis of AFL is associated with excessive lipid accumulation in hepatocytes. Resveratrol (RES), a dietary polyphenol found in red wines and grapes, has been shown to have a hepatoprotective effect. Autophagy is a crucial physiological process in cellular catabolism that involves the regulation of lipid droplets. Autophagy maintains a balance between protein synthesis, degradation and self-recycling...
2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27565442/calcium-sensing-receptor-effects-in-adipocytes-and-liver-cells-implications-for-an-adipose-hepatic-crosstalk
#4
Pia Villarroel, Pamela Mattar, Amanda D'Espessailles, Marco Arrese, Andrea Arreguin, Cecilia Fuentes, Marcela Reyes, Mariana Cifuentes
The calcium sensing receptor (CaSR) is expressed in human adipose cells, and its activation may associate with adipose tissue (AT) dysfunction. We evaluated whether CaSR stimulation influences adipocyte triglyceride (TG) and fatty acid binding protein 4 (aP2) content, and hepatocyte TGs and proinflammatory cytokine expression. The effect of the calcimimetic cinacalcet on TGs (fluorimetry), lipogenic genes (qPCR) and aP2 (immunoblot) was evaluated in LS14 adipocytes or AT. In the human HepG2 hepatic cell line, we assessed CaSR expression and cinacalcet effect on TGs and lipogenic and proinflammatory genes...
October 1, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27490779/swertiamarin-ameliorates-oleic-acid-induced-lipid-accumulation-and-oxidative-stress-by-attenuating-gluconeogenesis-and-lipogenesis-in-hepatic-steatosis
#5
Tushar P Patel, Komal Rawal, Sanket Soni, Sarita Gupta
Swertiamarin, a bitter secoiridoid glycoside, is an antidiabetic drug with lipid lowering activity meliorates insulin resistance in Type 2 Diabetes condition. Therefore, the study was designed to explore the antioxidant and hypolipidemic activity of swertiamarin in ameliorating NAFLD caused due to hepatic lipid accumulation, inflammation and insulin resistance. Steatosis was induced in HepG2 cells by supplementing 1mM oleic acid (OA) for 24h which was marked by significant accumulation of lipid droplets. This was determined by Oil Red O (ORO) staining and triglyceride accumulation...
August 1, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27456850/anti-lipoapoptotic-effects-of-alisma-orientalis-extract-on-non-esterified-fatty-acid-induced-hepg2-cells
#6
Hyeon-Soo Jeong, Young-Hwan Cho, Kang-Hoon Kim, Yumi Kim, Ki-Suk Kim, Yun-Cheol Na, Jiyoung Park, In-Seung Lee, Jang-Hoon Lee, Hyeung-Jin Jang
BACKGROUND: Liver steatosis was caused by lipid accumulation in the liver. Alisma orientale (AO) is recognized as a promising candidate with therapeutic efficacy for the treatment of nonalcoholic fatty liver disease (NAFLD). HepG2 hepatocyte cell line is commonly used for liver disease cell model. METHOD: The HepG2 cells were cultured with the NEFAs mixture (oleic and palmitic acids, 2:1 ratio) for 24 h to induce hepatic steatosis. Then different doses of Alisma orientale extract (AOE) was treated to HepG2 for 24 h...
2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27446914/in-vitro-modulation-of-the-lps-induced-proinflammatory-profile-of-hepatocytes-and-macrophages-approaches-for-intervention-in-obesity
#7
Ramiar K Kheder, James Hobkirk, Cordula M Stover
Low grade endotoxemia is a feature of obesity which is linked to development of steatohepatitis in non-alcoholic fatty liver disease. In this study, macrophages (J774) and hepatocytes (HepG2) were stimulated with lipopolysaccharide (LPS) from E. coli 0111: B4 and analyzed for modulation of this response when preconditioned or stimulated subsequent to LPS, with different doses of Vitamin D3 or docosahexaenoic acid (DHA) over a time period of 1 and 5 days. Pro-inflammatory TNFα and pro-fibrotic TGFβ released into the supernatants were measured by ELISA; qPCR was performed for Srebp-1c and PPARα mRNA (genes for products involved in fatty acid synthesis and catabolism, respectively)...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27438262/investigating-nonalcoholic-fatty-liver-disease-in-a-liver-on-a-chip-microfluidic-device
#8
Manuele Gori, Maria Chiara Simonelli, Sara Maria Giannitelli, Luca Businaro, Marcella Trombetta, Alberto Rainer
BACKGROUND AND AIM: Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disease worldwide, ranging from simple steatosis to nonalcoholic steatohepatitis, which may progress to cirrhosis, eventually leading to hepatocellular carcinoma (HCC). HCC ranks as the third highest cause of cancer-related death globally, requiring an early diagnosis of NAFLD as a potential risk factor. However, the molecular mechanisms underlying NAFLD are still under investigation. So far, many in vitro studies on NAFLD have been hampered by the limitations of 2D culture systems, in which cells rapidly lose tissue-specific functions...
2016: PloS One
https://www.readbyqxmd.com/read/27313828/s-propargyl-cysteine-exerts-a-novel-protective-effect-on-methionine-and-choline-deficient-diet-induced-fatty-liver-via-akt-nrf2-ho-1-pathway
#9
Wenwen Li, Fenfen Ma, Laiyin Zhang, Yong Huang, Xinghui Li, Aijie Zhang, Cuilan Hou, Yichun Zhu, YiZhun Zhu
This study investigated the antioxidative effect of S-propargyl-cysteine (SPRC) on nonalcoholic fatty liver (NAFLD) by treating mice fed a methionine and choline deficient (MCD) diet with SPRC for four weeks. We found that SPRC significantly reduced hepatic reactive oxygen species (ROS) and methane dicarboxylic aldehyde (MDA) levels. Moreover, SPRC also increased the superoxide dismutase (SOD) activity. By Western blot, we found that this protective effect of SPRC was importantly attributed to the regulated hepatic antioxidant-related proteins, including protein kinase B (Akt), heme oxygenase-1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf2), and cystathionine γ-lyase (CSE, an enzyme that synthesizes hydrogen sulfide)...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27275117/the-effect-of-chitooligosaccharides-on-oleic-acid-induced-lipid-accumulation-in-hepg2-cells
#10
Peiqiu Cao, Guidong Huang, Qingyun Yang, Jiao Guo, Zhengquan Su
This experiment aimed to evaluate the capacities of two types of chitooligosaccharides (COS) with different molecular weights for the ability to eliminate lipid accumulation in hepatocytes. We have established a lipid accumulation model in HepG2 cells for these studies in vitro, which was established by induction with oleic acid. The capacity of COS to eliminate lipid accumulation was evaluated using three metrics: the thiazolyl blue dye absorbance (MTT value), the morphology of intracellular lipid droplets and the triglyceride level (TG)...
May 2016: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/27265384/dihydromyricetin-ameliorates-oleic-acid-induced-lipid-accumulation-in-l02-and-hepg2-cells-by-inhibiting-lipogenesis-and-oxidative-stress
#11
Caifeng Xie, Zhen Chen, Chengfu Zhang, Xin Xu, Jiangbo Jin, Weihua Zhan, Tianyu Han, Jianbin Wang
AIMS: Dihydromyricetin (DMY), a flavonoid component isolated from Ampelopsis grossedentata, was recently reported to ameliorate nonalcoholic fatty liver disease (NAFLD) in patients. However, the underlying mechanisms of this action remain unknown. Here, we evaluate the effect of DMY on an in vitro model of NAFLD and investigate the signal transduction pathways underlying DMY treatment. MAIN METHODS: Oleic acid (OA) induced hepatic steatosis was established in L02 and HepG2 cells as in vitro model of NAFLD...
July 15, 2016: Life Sciences
https://www.readbyqxmd.com/read/27237960/lipid-droplets-affect-elimination-of-porphyromonas-gingivalis-in-hepg2-cells-by-altering-the-autophagy-lysosome-system
#12
Yumi Zaitsu, Mayumi Iwatake, Keiko Sato, Takayuki Tsukuba
Recent studies have shown that infection with Porphyromonas gingivalis, a major periodontal pathogen, hastens the progression of non-alcoholic fatty liver disease (NAFLD). However, the intracellular fate of P. gingivalis in hepatocytes remains unknown. Here, using oleic-acid-induced HepG2 cells as an in vitro model for NAFLD, we found that lipid droplets increased the existence of P. gingivalis in the cells at an early phase of infection. Confocal microscopic analysis revealed that lipid droplets affected the formation of autolysosomes in infected cells...
September 2016: Microbes and Infection
https://www.readbyqxmd.com/read/27176782/treatment-with-estrogen-protects-against-ovariectomy-induced-hepatic-steatosis-by-increasing-aqp7-expression
#13
Xiaohua Fu, Lili Xing, Weihai Xu, Jing Shu
Recent evidence has suggested that the marked decrease in ovarian secretion of estrogens in postmenopausal women may be associated with the development of non-alcoholic fatty liver disease. The present study aimed to elucidate the mechanisms by which low levels of estrogen induce fatty liver disease using an ovariectomized (OVX) mouse model and an in vitro cell model. A total of 24 female C57/BL6 mice were divided into four groups: Sham operation, sham operation plus subcutaneous implantation of tamoxifen (TAM), bilateral OVX, and OVX plus subcutaneous implantation of 17β-estradiol (E2)...
July 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27123455/the-compound-of-mangiferin-berberine-salt-has-potent-activities-in-modulating-lipid-and-glucose-metabolisms-in-hepg2-cells
#14
Can Wang, Jian-Dong Jiang, Wei Wu, Wei-Jia Kong
The mangiferin-berberine (MB) salt was synthesized by ionic bonding of mangiferin (M) and berberine (B) at an equal molecular ratio. This study aimed to investigate the activities of MB salt in modulating lipid and glucose metabolisms in HepG2 cells. After 24 h treatment of the studying compounds, cellular AMP-activated protein kinase α (AMPKα)/acetyl-CoA carboxylase (ACC) protein levels and carnitine palmitoyltransferase (CPT) 1 activities, intracellular lipid contents, mRNA expression levels of target genes, glucose consumption, and glucose production amounts were determined...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27084371/pinolenic-acid-downregulates-lipid-anabolic-pathway-in-hepg2-cells
#15
Ah Ron Lee, Sung Nim Han
Pine nut oil (PNO) was reported to reduce lipid accumulation in the liver. However, the specific effect of pinolenic acid (18:3, all-cis-Δ5,9,12), a unique component of PNO, on lipid metabolism has not been studied. We hypothesized that pinolenic acid downregulates the lipid anabolic pathway in HepG2 cells. HepG2 cells were incubated in serum-free medium supplemented with 50 μM bovine serum albumin (BSA), palmitic acid, oleic acid, γ-linolenic acid, pinolenic acid, eicosapentaenoic acid (EPA), or α-linolenic acid for 24 h...
July 2016: Lipids
https://www.readbyqxmd.com/read/27081834/artemisia-capillaris-formula-inhibits-hepatic-steatosis-via-an-mir%C3%A2-122%C3%A2-induced-decrease-in-fatty-acid-synthase-expression-in%C3%A2-vivo-and-in%C3%A2-vitro
#16
Liya Liu, Jinyan Zhao, Ying Li, Yun Wan, Jiumao Lin, Aling Shen, Wei Xu, Huang Li, Yuchen Zhang, Jianfeng Xu, Jun Peng, Zhenfeng Hong
Non‑alcoholic fatty liver disease (NAFLD) is a widespread health concern, and there is currently insufficient understanding regarding its pathogenesis and treatment. The present study aimed to explore the effects of Artemisia capillaris formula (ACF) on high‑fat diet‑induced hepatic steatosis and fatty acid‑induced intracellular lipid accumulation, by micro (mi)RNA regulation. A total of 72 Sprague‑Dawley rats were divided into six groups (n=12/group). One group was designated as the control group and fed a normal diet, and the remaining five groups were allowed ad libitum access to a high‑fat diet for eight weeks, in order to establish an NAFLD rat model...
June 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27073470/low-simvastatin-concentrations-reduce-oleic-acid-induced-steatosis-in-hepg2-cells-an-in-vitro-model-of-non-alcoholic-fatty-liver-disease
#17
Mohammad J Alkhatatbeh, Lisa F Lincz, Rick F Thorne
Non-alcoholic fatty liver disease (NAFLD) is an inflammatory condition caused by hepatic lipid accumulation that is associated with insulin resistance, diabetes and metabolic syndrome. Although statins should be used with caution in liver diseases, they are increasingly investigated as a possible treatment for NAFLD. The present study recreated an in vitro model of NAFLD using HepG2 cells exposed to oleic acid (OA), which was used to quantify OA-induced lipid accumulation in HepG2 cells treated with various concentrations of simvastatin...
April 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/26869449/lipid-accumulation-stimulates-the-cap-independent-translation-of-srebp-1a-mrna-by-promoting-hnrnp-a1-binding-to-its-5-utr-in-a-cellular-model-of-hepatic-steatosis
#18
Luisa Siculella, Romina Tocci, Alessio Rochira, Mariangela Testini, Antonio Gnoni, Fabrizio Damiano
Non-alcoholic fatty liver disease (NAFLD) is a chronic disease characterized by accumulation of lipid droplets in hepatocytes. Enhanced release of non-esterified fatty acids from adipose tissue accounts for a remarkable fraction of accumulated lipids. However, the de novo lipogenesis (DNL) is also implicated in the etiology of the NAFLD. Sterol Regulatory Element-Binding Protein-1 (SREBP-1) is a transcription factor modulating the expression of several lipogenic enzymes. In the present study, in order to investigate the effect of lipid droplet accumulation on DNL, we used a cellular model of steatosis represented by HepG2 cells cultured in a medium supplemented with free oleic and palmitic fatty acids (FFAs)...
May 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/26828452/a-new-hexacyclic-triterpene-acid-from-the-roots-of-euscaphis-japonica-and-its-inhibitory-activity-on-triglyceride-accumulation
#19
Yan-Ci Li, Ke Tian, Li-Juan Sun, Hui Long, Lu-Jun Li, Zheng-Zhi Wu
A new taraxerene-type hexacyclic triterpene acid named (12R,13S)-3-methoxy-12,13-cyclo-taraxerene-2,14-diene-1-one-28-oic acid (1), together with a known compound 3,7-dihydroxy-5-octanolide (2), was isolated from the roots of Euscaphis japonica. The structure of new compound 1 was elucidated on the basis of NMR, HR-ESIMS and X-ray diffraction analysis. It showed promising inhibitory activity on oleic acid induced triglyceride accumulation on HepG2 cells.
March 2016: Fitoterapia
https://www.readbyqxmd.com/read/26794005/pu-erh-tea-extract-ameliorates-high-fat-diet-induced-nonalcoholic-steatohepatitis-and-insulin-resistance-by-modulating-hepatic-il-6-stat3-signaling-in-mice
#20
Xianbin Cai, Chongye Fang, Shuhei Hayashi, Shumei Hao, Mingming Zhao, Hiroko Tsutsui, Shuhei Nishiguchi, Jun Sheng
BACKGROUND: Pu-erh tea, made from the leaves of Camellia sinensis, possesses activities beneficial for human health, including anti-inflammatory, anti-oxidant, and anti-obesity properties. OBJECTIVE: We investigated the effects of a pu-erh tea extract (PTE) on nonalcoholic steatohepatitis (NASH) and the molecular mechanisms underlying such effects. METHODS: Eight-week-old male C57BL/6J mice were fed a normal chow diet or high-fat diet (HFD) for 17 weeks, during which PTE was simultaneously administered in drinking water...
August 2016: Journal of Gastroenterology
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