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Oleic acid hepg2

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https://www.readbyqxmd.com/read/28954528/recombinant-human-cytoglobin-prevents-atherosclerosis-by-regulating-lipid-metabolism-and-oxidative-stress
#1
Lingling Ou, Xin Li, Baihong Chen, Zhenhuang Ge, Junyi Zhang, Ye Zhang, Gaotai Cai, Zhen Li, Ping Wang, Wenqi Dong
Atherosclerosis is a chronic inflammatory vascular disease characterized by lipid accumulation and endothelial dysfunction. Cytoglobin has been shown to exert protective effects under oxidative stress conditions. The aim of this study was to determine whether recombinant human cytoglobin (rhCYGB) has protective effects against atherosclerosis. We intraperitoneally injected rhCYGB (0, 4, or 7 mg/kg BW) into the atherosclerotic rats daily for 60 days. The rhCYGB injections reduced low-density lipoprotein cholesterol (LDL-C) levels and increased high-density lipoprotein cholesterol levels in a dose-dependent manner, rhCYGB (7 mg/kg) significantly attenuated atherosclerosis...
January 1, 2017: Journal of Cardiovascular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28938582/effect-of-hepatic-steatosis-on-the-progression-of-chronic-hepatitis-b-a-prospective-cohort-and-in-vitro-study
#2
Yangqin Chen, Chunlei Fan, Yuhan Chen, Hui Liu, Shanshan Wang, Peiling Dong, Lei Li, Huiguo Ding
AIM: To characterize the effect of hepatic steatosis (HS) on the progression of chronic hepatitis B. METHODS: A total of 162 chronic hepatitis B (CHB) patients confirmed by liver biopsy were involved in this study. All subjects were prospectively followed-up for 5 years in real-life clinical practice. Fibrosis stage was determined using aspartate aminotransferase-to-platelet ratio index (APRI). The end-point was cirrhosis, liver cancer or death. The effects of steatosis on the biological behavior of hepatocellular carcinoma cells were investigated using oleic acid-induced lipid accumulation in HepG2, HLE, PLC, and SMMC-7721 cells...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28924084/comparison-of-the-effect-of-trans-fatty-acid-isomers-on-apolipoprotein-a1-and-b-secretion-in-hepg2-cells
#3
Koji Nagao, Kazuaki Yoshinaga, Toshiharu Nagai, Hoyo Mizobe, Akihiko Yoshida, Fumiaki Beppu, Naohiro Gotoh
Intake of trans fatty acid (TFA) is believed to change the ratio of low-density lipoprotein (LDL) to high density lipoprotein (HDL) cholesterol in blood, which leads to cardiovascular disease. In this study, thirteen types of TFA including monoene type TFA (trans-octadecenoic fatty acid isomers, t-18:1 isomers), diene type TFA (t9,t12-18:2), and triene type TFA (t-18:3) were added to cultured HepG2 cells to compare the amount of apolipoprotein A1 and B (those relating to levels of HDL and LDL cholesterol in blood, respectively) being secreted...
October 1, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28759727/the-beneficial-effects-of-resveratrol-on-steatosis-and-mitochondrial-oxidative-stress-in-hepg2-cells
#4
Magdalena Izdebska, Iwona Piątkowska-Chmiel, Agnieszka Korolczuk, Mariola Herbet, Monika Gawrońska-Grzywacz, Renata Gieroba, Marcin Sysa, Klaudia Czajkowska-Bania, Marcelina Cygal, Agnieszka Korga, Jarosław Dudka
Nonalcoholic fatty liver disease (NAFLD) is currently one of the most common chronic liver diseases, especially in developed countries. One group of substances with a potential use in the treatment of NAFLD are plant polyphenols, represented by resveratrol. The aim of this study was to evaluate the effect of resveratrol on steatosis and oxidative stress in HepG2 cells. The steatosis of cells was carried out using free fatty acids: oleic or palmitic acid and their mixtures. Steatosis was visualized using the intracellular lipid staining by Nile Red dye with a fluorescence microscope...
July 31, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28737815/isofraxidin-a-coumarin-component-improves-high-fat-diet-induced-hepatic-lipid-homeostasis-disorder-and-macrophage-inflammation-in-mice
#5
Jian Li, Xiaofei Li, Zhike Li, Lu Zhang, Yonggang Liu, Hong Ding, Shanye Yin
Isofraxidin (IF) is a coumarin compound produced in the functional foods Siberian ginseng and Apium graveolens. The first objective of this study was to investigate the protective effects and putative methods of IF in combating lipotoxicity in vitro and in vivo. Oleic acid was used to induce lipid turbulence in human hepatoma cells (HepG2). Alterations in triglyceride metabolism, inflammation and oxidative status were monitored. Results show that IF mainly reduced triglyceride accumulation, TNF-α release and ROS activation in metabolic disordered cells...
August 1, 2017: Food & Function
https://www.readbyqxmd.com/read/28707223/inhibition-of-exendin-4-induced-steatosis-by-protein-kinase-a-in-cultured-hepg2-human-hepatoma-cells
#6
Alice Y Chen-Liaw, Gabrielle Hammel, George Gomez
Nonalcoholic fatty liver is characterized by the abnormal accumulation of triglycerides within hepatocytes, resulting in a steatotic liver. Glucagon-like peptide 1 and its analog exendin-4 can ameliorate certain aspects of this syndrome by inducing weight loss and reducing hepatic triglyceride accumulation, but it is unclear whether these effects result from the effects of glucagon-like peptide 1 on the pancreas, or from direct action on the liver. This study investigated the direct action and putative cellular mechanism of exendin-4 on steatotic hepatocytes in culture...
July 13, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28659743/in-vitro-inhibition-of-lipid-accumulation-induced-by-oleic-acid-and-in-vivo-pharmacokinetics-of-chitosan-microspheres-ctms-and-chitosan-capsaicin-microspheres-ccms
#7
Sihui Wu, Haitao Pan, Sirong Tan, Chen Ding, Guidong Huang, Guihua Liu, Jiao Guo, Zhengquan Su
Chitosan and capsaicin are compounds extracted from natural products and have been indicated to lower body weight and prevent fatty liver. However, their applications are limited by poor oral bioavailability, low compliance and some serious side effects. To solve these problems, we successfully prepared chitosan microspheres (CTMS) and chitosan-capsaicin microspheres (CCMS) in previous study. Therefore, in the present study, we evaluated the ability of CTMS and CCMS to eliminate lipid accumulation in hepatocytesand also characterized their pharmacokinetic parameters after administration...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28659476/entry-and-release-of-hepatitis-c-virus-in-polarized-human-hepatocytes
#8
Sandrine Belouzard, Adeline Danneels, Lucie Fénéant, Karin Séron, Yves Rouillé, Jean Dubuisson
Hepatitis C virus (HCV) infects primarily hepatocytes that are highly polarized cells. The relevance of cell polarity in the HCV life cycle has only been addressed in distant models and remains poorly understood. Although polarized epithelial cells have a rather simple morphology with a basolateral and an apical domain, hepatocytes exhibit complex polarization structures. However, it has been reported that some selected polarized HepG2 cell clones can exhibit a honeycomb pattern of distribution of the tight-junction proteins, typical of columnar polarized epithelia which can be used as a simple model to study the role of cell polarization in viral infection of hepatocytes...
June 28, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28628909/nonalcoholic-fatty-liver-disease-impairs-the-cytochrome-p-450-dependent-metabolism-of-%C3%AE-tocopherol-vitamin-e
#9
Desirée Bartolini, Pierangelo Torquato, Carolina Barola, Angelo Russo, Chiara Rychlicki, Danilo Giusepponi, Guido Bellezza, Angelo Sidoni, Roberta Galarini, Gianluca Svegliati-Baroni, Francesco Galli
This study aims to investigate in in vivo and in vitro models of nonalcoholic fatty liver disease (NAFLD) the enzymatic metabolism of α-tocopherol (vitamin E) and its relationship to vitamin E-responsive genes with key role in the lipid metabolism and detoxification of the liver. The experimental models included mice fed a high-fat diet combined or not with fructose (HFD+F) and HepG2 human hepatocarcinoma cells treated with the lipogenic agents palmitate, oleate or fructose. CYP4F2 protein, a cytochrome P-450 isoform with proposed α-tocopherol ω-hydroxylase activity, decreased in HFD and even more in HFD+F mice liver; this finding was associated with increased hepatic levels of α-tocopherol and decreased formation of the corresponding long-chain metabolites α-13-hydroxy and α-13-carboxy chromanols...
June 7, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28602867/effect-of-gangjihwan-on-hepatic-steatosis-and-inflammation-in-high-fat-diet-fed-mice
#10
Jong Seong Roh, Haerim Lee, Jonghoon Lim, Jeongjun Kim, Heejung Yang, Yooshik Yoon, Soon Shik Shin, Michung Yoon
ETHNOPHARMACOLOGICAL RELEVANCE: Gangjihwan (DF), a polyherbal drug composed of Ephedra intermedia Schrenk et C. A. Mayer (Ephedraceae), Lithospermum erythrorhizon Siebold et Zuccarini (Borraginaceae), and Rheum palmatum L. (Polygonaceae), is used to treat obesity in local Korean clinics. The constituents of DF have traditionally been reported to exert anti-obesity and anti-nonalcoholic fatty liver disease (NAFLD) effects. Thus, we investigated the effects of DF on obesity and NAFLD and the underlying mechanisms...
July 12, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28577437/rutin-exhibits-hepatoprotective-effects-in-a-mouse-model-of-non-alcoholic-fatty-liver-disease-by-reducing-hepatic-lipid-levels-and-mitigating-lipid-induced-oxidative-injuries
#11
Qingsheng Liu, Ran Pan, Lei Ding, Fuli Zhang, Linfeng Hu, Bin Ding, Linwensi Zhu, Yongliang Xia, Xiaobing Dou
Nonalcoholic fatty liver disease (NAFLD) is characterized by excessive accumulation of hepatic lipids and oxidative injury of hepatocytes. Rutin is a natural flavonoid with significant roles in combating cellular oxidative stress and regulating lipid metabolism. The current study aims to investigate the molecular mechanisms underlying rutin's hypolipidemic and hepatoprotective effects in nonalcoholic fatty liver disease. Rutin treatment was applied to male C57BL/6 mice maintained on a high-fat diet and HepG2 cells challenged with oleic acid...
August 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28555106/high-fat-diet-induced-hepatic-18-carbon-fatty-acids-accumulation-up-regulates-cyp2a5-cyp2a6-via-nf-e2-related-factor-2
#12
Xing-He Wang, Xiao-Xu Cui, Xiao-Qi Sun, Xing-Hui Wang, Xiao-Chong Li, Yue Qi, Wei Li, Mei-Yu Han, Ishfaq Muhammad, Xiu-Ying Zhang
To investigate the role of hepatic 18-carbon fatty acids (FA) accumulation in regulating CYP2A5/2A6 and the significance of Nrf2 in the process during hepatocytes steatosis, Nrf2-null, and wild type mice fed with high-fat diet (HFD), and Nrf2 silenced or over expressed HepG2 cells administered with 18-carbon FA were used. HE and Oil Red O staining were used for mice hepatic pathological examination. The mRNA and protein expressions were measured with real-time PCR and Western blot. The results showed that hepatic CYP2A5 and Nrf2 expression levels were increased in HFD fed mice accompanied with hepatic 18-carbon FA accumulation...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28534819/ginsenoside-rb2-alleviates-hepatic-lipid-accumulation-by-restoring-autophagy-via-induction-of-sirt1-and-activation-of-ampk
#13
Qi Huang, Ting Wang, Liu Yang, He-Yao Wang
Although Panax ginseng is a famous traditional Chinese medicine and has been widely used to treat a variety of metabolic diseases including hyperglycemia, hyperlipidemia, and hepatosteatosis, the effective mediators and molecular mechanisms remain largely unknown. In this study we found that ginsenoside Rb2, one of the major ginsenosides in Panax ginseng, was able to prevent hepatic lipid accumulation through autophagy induction both in vivo and in vitro. Treatment of male db/db mice with Rb2 significantly improved glucose tolerance, decreased hepatic lipid accumulation, and restored hepatic autophagy...
May 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28533555/scopolin-ameliorates-high-fat-diet-induced-hepatic-steatosis-in-mice-potential-involvement-of-sirt1-mediated-signaling-cascades-in-the-liver
#14
Ahyoung Yoo, Vikram P Narayan, Eun Young Hong, Wan Kyunn Whang, Taesun Park
The present study aimed to investigate whether scopolin exhibits beneficial effects on high-fat diet (HFD)-induced hepatic steatosis in mice. The involvement of sirtuin 1 (SIRT1) as a molecular target for scopolin was also explored. Scopolin decreased the Km of SIRT1 for p53 and nicotinamide adenine dinucleotide without altering Vmax in a cell-free system. Scopolin alleviated oleic acid-induced lipid accumulation and downregulation of SIRT1 activity in HepG2 cells, and these beneficial effects of scopolin were abolished in the presence of SIRT1 inhibitor...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28524760/evaluation-of-the-cytotoxicity-hepg2-and-chemical-composition-of-polar-extracts-from-the-ruderal-species-coleostephus-myconis-l-rchb-f
#15
Sílvia M F Bessada, João C M Barreira, J Santos, Carla Costa, Filipa B Pimentel, Maria João Bessa, João Paulo Teixeira, M Beatriz P P Oliveira
Coleostephus myconis (L.) Rchb.f. (Asteraceae) is a highly disseminated plant species with ruderal and persistent growth. Owing to its advantageous agronomic properties, C. myconis might have industrial applications. However, this species needs to be comprehensively characterized before any potential use. In a previous study, the phenolic composition and antioxidant activity of different C. myconis tissues were characterized. This investigation was extended to examine the cytotoxic potential of selected plant tissues (flowers and green parts) using a HepG2 cell line by utilizing the lysosomal neutral red uptake assay or mitochondrial (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay...
2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28512733/a-lipidomic-cell-based-assay-for-studying-drug-induced-phospholipidosis-and-steatosis
#16
Juan Carlos García-Cañaveras, Manuel David Peris-Díaz, M Isabel Alcoriza-Balaguer, Manuela Cerdán-Calero, M Teresa Donato, Agustín Lahoz
Phospholipidosis and steatosis are two toxic effects, which course with overaccumulation of different classes of lipids in the liver. MS-based lipidomics has become a powerful tool for the comprehensive determination of lipids. LC-MS lipid profiling of HepG2 cells is proposed as an in vitro assay to study and anticipate phospholipidosis and steatosis. Cells with and without preincubation with a mixture of free fatty acids (FFA; i.e. oleic and palmitic) were exposed to a set of well-known steatogenic and phospholipidogenic compounds...
September 2017: Electrophoresis
https://www.readbyqxmd.com/read/28500841/effect-of-hepatic-steatosis-on-the-progression-of-chronic-hepatitis-b-a-prospective-cohort-and-in-vitro-study
#17
Yangqin Chen, Chunlei Fan, Yuhan Chen, Hui Liu, Shanshan Wang, Peiling Dong, Lei Li, Huiguo Ding
AIM: To characterize the effect of hepatic steatosis (HS) on the progression of chronic hepatitis B. METHODS: A total of 162 chronic hepatitis B (CHB) patients confirmed by liver biopsy were involved in this study. All subjects were prospectively followed-up for 5 years in real-life clinical practice. Fibrosis stage was determined using aspartate aminotransferase-to-platelet ratio index (APRI). The end-point was cirrhosis, liver cancer or death. The effects of steatosis on the biological behavior of hepatocellular carcinoma cells were investigated using oleic acid-induced lipid accumulation in HepG2, HLE, PLC, and SMMC-7721 cells...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28449683/inhibition-of-nampt-aggravates-high-fat-diet-induced-hepatic-steatosis-in-mice-through-regulating-sirt1-ampk%C3%AE-srebp1-signaling-pathway
#18
Ling-Fang Wang, Xiao-Nv Wang, Cong-Cong Huang, Long Hu, Yun-Fei Xiao, Xiao-Hui Guan, Yi-Song Qian, Ke-Yu Deng, Hong-Bo Xin
BACKGROUND: Nonalcoholic fatty liver disease is one of the most common liver diseases in the world and is a typical hepatic manifestation of metabolic syndrome which is characterized with lipid accumulation in liver. Nicotinamide phosphoribosyltransferase (NAMPT) has been recently identified as an enzyme involved in nicotinamide adenine dinucleotide (NAD(+)) biosynthesis and plays an important role in cellular metabolism in variety of organs in mammals. The aim of this study was to investigate the effects of NAMPT on high fat diet-induced hepatic steatosis...
April 27, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28415630/resveratrol-alleviates-ffa-and-ccl4-induced-apoptosis-in-hepg2-cells-via-restoring-endoplasmic-reticulum-stress
#19
Fenghua Li, Yang Yang, Lili Yang, Kai Wang, Xing Zhang, Yunlong Zong, Yixin Ding, Changhong Wang, Li Zhang, Guang Ji
Cell apoptosis often induces inflammation and injury in the liver, with endoplasmic reticulum (ER) stress as the most possible reason. Resveratrol (RSV) has been shown to prevent hepatic steatosis and alleviate apoptosis, however, the exact mechanisms underlying the effects still need to be explored. Here we co-cultured HepG2 cells with free fatty acid (FFA) solution (oleic acid: palmitic acid = 2:1) and then exposed to a carbon tetrachloride (CCl4) solution to induce apoptosis. To evaluate the therapeutic effects, RSV (2...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28317281/comparison-of-dietary-polyphenols-for-protection-against-molecular-mechanisms-underlying-nonalcoholic-fatty-liver-disease-in-a-cell-model-of-steatosis
#20
Hossein Rafiei, Kosar Omidian, Brian Bandy
SCOPE: Dietary polyphenols have shown promise in protecting the liver against nonalcoholic fatty liver disease. The relative effectiveness and mechanisms of different polyphenols however is mostly unknown. METHODS AND RESULTS: In a model of steatosis using HepG2 hepatocytes, we evaluated the protective effects of different classes of polyphenols and the contributing mechanisms. The treatment of the cells with oleic acid increased reactive oxygen species (ROS) generation and expression of tumor necrosis factor alpha (TNF-α), decreased expression of uncoupling protein 2, and decreased mitochondrial content and markers of biogenesis...
March 20, 2017: Molecular Nutrition & Food Research
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