Read by QxMD icon Read

Oleic acid hepg2

Makoto Takeda, Takanori Sakaguchi, Takanori Hiraide, Yasushi Shibasaki, Yoshifumi Morita, Hirotoshi Kikuchi, Koji Ikegami, Mitsutoshi Setou, Hiroyuki Konno, Hiroya Takeuchi
The molecular features of hepatocellular carcinoma arising from non-alcoholic fatty liver disease (NAFLD-HCC) are not well known. In this study, we investigated the mechanism by which NAFLD-HCC survives in a fat-rich environment. We found that caveolin (CAV)-1 was overexpressed in clinical specimens from NAFLD-HCC patients. HepG2, HLE, and HuH-7 HCC cell lines showed decreased proliferation in the presence of the saturated fatty acids palmitic acid and stearic acid, although only HLE cells expressed high levels of CAV-1...
June 13, 2018: Cancer Science
Myung-Ji Kang, Eun-Bin Kwon, Hyung Won Ryu, Seoghyun Lee, Jae-Won Lee, Doo-Young Kim, Mi Kyeong Lee, Sei-Ryang Oh, Hyun-Sun Lee, Su Ui Lee, Mun-Ock Kim
The extract tea of Dendropanax morbifera is popular beverages in Korea, and their preventive and therapeutic roles in metabolic disorders have been reported. However, the molecular mechanism has not been studied despite the known efficacy of D. morbifera . Eleven fractions (fr.1-fr.11) were divided by MPLC to find the active compound. Among them, Fr.5 was superior to others in that the inhibitory efficacy of de novo triglyceride (TG) biosynthesis. NMR analysis revealed that Fr.5 is composed 98% or more (9 Z ,16 S )-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid (HOD)...
2018: Frontiers in Pharmacology
Qingqing Yu, Yayun Liu, Yufei Wu, Yong Chen
AIMS: Curcumin is a polyphenol compound with many pharmacological activities including antioxidant, lipid-loweing and liver protective. Dihydrocurcumin (DHC) is one of the major metabolites of curcumin. So far, the pharmacological activity of DHC has not been reported. Here, we evaluate the effects of DHC on oleic acid (OA)-induced lipid accumulation, oxidative stress and insulin resistance and the underlying mechanism in L02 and HepG2 cells. MAIN METHODS: OA-induced L02 and HepG2 cells were used as the in vitro model of nonalcoholic fatty liver disease (NAFLD)...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
B Huang, J Bao, Y-R Cao, H-F Gao, Y Jin
Nonalcoholic fatty liver disease (NAFLD) is a chronic hepatic disease associated with excessive accumulation of lipids in hepatocytes. As the disease progresses, oxidative stress plays a pivotal role in the development of hepatic lipid peroxidation. Cytochrome P450 1A1 (CYP1A1), a subtype of the cytochrome P450 family, has been shown to be a vital modulator in production of reactive oxygen species. However, the exact role of CYP1A1 in NAFLD is still unclear. The aim of this study was to investigate the effects of CYP1A1 on lipid peroxidation in oleic acid (OA)-treated human hepatoma cells (HepG2)...
May 2018: Biochemistry. Biokhimii︠a︡
Ying Liu, Yajun Cheng, Jinwei Li, Yuanpeng Wang, Yuanfa Liu
In the present study, effects of cis-9,10-epoxy stearic acid (ESA) generated by the thermal oxidation of oleic acid on HepG2 cells including cytotoxicity, apoptosis and oxidative stress were investigated. Our results revealed that ESA decreased the cell viability and induced cell death. Cell cycle analysis with propidium iodide staining showed that ESA induced cell cycle arrest at the G0/G1 phase in HepG2 cells. Cell apoptosis analysis with annexin V and propidium iodide staining demonstrated that ESA induced HepG2 cell apoptotic events in a dose- and time-dependent manner, the apoptosis of cells after treated with 500 μM of ESA for 12 h, 24 h and 48 h was 32...
May 6, 2018: Journal of Agricultural and Food Chemistry
Ruijie Gong, Xiaofei Lv, Fengqiong Liu
Background: The miRNA cluster miR-17-92 is known to act as an oncogene in various cancers. Members of this cluster were also found to be involved in some other pathological process, such as steatosis, which is a pivotal event in the initiation and progression of nonalcoholic fatty liver disease (NAFLD). This study aimed to explore whether miR-17, one of the most functional miRNAs in the miR-17-92 family, participates in the process of steatosis in hepatoma cells. Methods: We developed both a miR-17-expressing transgenic mouse model and a miR-17-expressing HepG2 cell model, the latter was established via stable transfection...
2018: Cellular & Molecular Biology Letters
Xuanming Chen, Linzhao Li, Xiaohong Liu, Ruixi Luo, Guangneng Liao, Lan Li, Jingping Liu, Jingqiu Cheng, Yanrong Lu, Younan Chen
Aim This study aims to demonstrate the protective effects of monounsaturated oleic acid (OA) against saturated palmitic acid (PA) induced cellular lipotoxicity in hepatocytes and rats with non-alcoholic steatohepatitis (NASH). MAIN METHODS: Human hepatoma cell line HepG2 cells and neonatal rat primary hepatocytes were treated with PA or/and OA for 24 h. SD rats were fed with high fat diet (HFD) to induce NASH. From the 16th w, the HFD was full or half replaced by olive oil to observe the protective effects...
June 15, 2018: Life Sciences
Paweł Paśko, Małgorzata Tyszka-Czochara, Agnieszka Galanty, Joanna Gdula-Argasińska, Paweł Żmudzki, Henryk Bartoń, Paweł Zagrodzki, Shela Gorinstein
The study compares lyophilized broccoli sprouts and florets in terms of their chemical composition, cytotoxic and proapoptotic potential against hepatocellular carcinoma HepG2, colorectal cancer SW480, and skin fibroblast BJ cells. Sinapic and isochlorogenic acids were predominant phenolics in the sprouts and florets, respectively. The amount of sulforaphane in the sprouts was significantly higher vs. florets. Oleic and linoleic acids dominated in the sprouts, while caproic, stearic and oleic acids in the florets...
June 2018: Plant Foods for Human Nutrition
Elaine Wat, Yanping Wang, Ken Chan, Hon Wai Law, Chi Man Koon, Kit Man Lau, Ping Chung Leung, Choly Yan, Clara Bik San Lau
BACKGROUND: Metabolic syndrome is the cluster of risk factors that leads to increased episodes of cardiovascular disease (CVD). These risk factors include but are not limited to obesity, non-alcoholic fatty liver (NAFLD), dyslipidemia, and type 2 diabetes. Since the pathogenesis of metabolic syndrome has multiple metabolic origins, there is no single treatment for it. Pharmacological approaches consist of separate drugs which target at individual risk factors which pose various side effects...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Jin-Taek Hwang, Eun Ju Shin, Min-Yu Chung, Jae Ho Park, Sangwon Chung, Hyo-Kyoung Choi
BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver disease and is closely associated with metabolic syndrome. In the present study, we observed the effect of ethanol extract of Allium fistulosum (EAF) on NAFLD and have suggested the possibility of using EAF as a natural product for application in the development of a treatment for NAFLD. MATERIALS/METHODS: The preventive effect on hepatic lipid accumulation was estimated by using an oleic acid (OA)-induced NAFLD model in vitro and a Western diet (high-fat high-sucrose; WD)-induced obese mouse model...
April 2018: Nutrition Research and Practice
Eun-Bin Kwon, Myung-Ji Kang, Soo-Yeon Kim, Yong-Moon Lee, Mi-Kyeong Lee, Heung Joo Yuk, Hyung Won Ryu, Su Ui Lee, Sei-Ryang Oh, Dong-Oh Moon, Hyun-Sun Lee, Mun-Ock Kim
Zanthoxylum ailanthoides (ZA) has been used as folk medicines in East Asian and recently reported to have several bioactivity; however, the studies of ZA on the regulation of triacylglycerol (TG) biosynthesis have not been elucidated yet. In this study, we examined whether the methanol extract of ZA (ZA-M) could reduce oleic acid- (OA-) induced intracellular lipid accumulation and confirmed its mode of action in HepG2 cells. ZA-M was shown to promote the phosphorylation of AMPK and its upstream LKB1, followed by reduction of lipogenic gene expressions...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Tianxing Chen, Dong Yan, Xiaoying Cheng, XiaoJun Ji, Jinjun Bian, Wu Yin
MicroRNAs are potential therapeutic targets for metabolic diseases. Here, miR-1224-5p was highly expressed in the livers of mice fed a high-fat diet (HFD) and in obese (ob/ob) mice. To examine the potential role of miR-1224-5p, we constructed liver-specific adenoviral vectors expressing either an miR-1224-5p inhibitor sequence or miR-1224-5p mimic sequences. After tail-vein vector injection, HFD-fed mice were examined for expression of lipogenic genes. We found that miR-1224-5p inhibitors significantly attenuated hepatic lipogenesis and steatosis in HFD-fed mice, whereas miR-1224-5p mimicked promoted lipid accumulation in the liver of chow-fed C57BL/6 mice...
May 1, 2018: Endocrinology
Runmei Yang, Xinxin Chu, Le Sun, Zhuoying Kang, Min Ji, Ying Yu, Ying Liu, Zhendan He, Nannan Gao
The aim of this study was to evaluate the hypolipidemic effect and mechanisms of total phenylpropanoid glycosides extracted from Ligustrum robustum (Roxb.) Blume (LRTPG) in hamsters fed a high-fat diet and to discover bioactive components in HepG2 cell model induced by oleic acid. LRTPG of high (1.2 g/kg), medium (0.6 g/kg), and low (0.3 g/kg) doses was administrated daily for 21 consecutive days in hamsters. We found that in hamsters fed a high-fat diet, LRTPG effectively reduced the concentrations of plasma triglycerides (TG), free fatty acid, total cholesterol, low-density lipoprotein cholesterol, and hepatic TG and total cholesterol...
April 2018: Phytotherapy Research: PTR
Ye Tian, Haihua Feng, Lu Han, Lin Wu, Hongming Lv, Bingyu Shen, Zheng Li, Qiaoling Zhang, Guowen Liu
Magnolol (MG) is a kind of lignin isolated from Magnolia officinalis , which serves several different biological functions, such as antifungal, anticancer, antioxidant, and hepatoprotective functions. This study aimed to evaluate the protective effect of MG against oleic acid (OA)-induced hepatic steatosis and inflammatory damage in HepG2 cells and in a tyloxapol (Ty)-induced hyperlipidemia mouse model. Our findings indicated that MG can effectively inhibit OA-stimulated tumor necrosis factor α (TNF-α) secretion, reactive oxygen species generation, and triglyceride (TG) accumulation...
2018: Frontiers in Immunology
Anna M Weijler, Barbara Schmidinger, Stylianos Kapiotis, Hilde Laggner, Marcela Hermann
Non-alcoholic fatty liver disease (NAFLD) is marked by hepatic fat accumulation and reflects a spectrum of chronic liver diseases associated with obesity, impaired insulin sensitivity and dyslipidemia. Apolipoprotein O (ApoO) is a new member of the plasma apolipoprotein family that may play a role in lipid metabolism and electron transport activity of the mitochondrium. However, its physiological functions have not been elucidated yet. Based on our previous data in a non-mammalian experimental system [1], we hypothesized that hepatic expression of ApoO is tightly linked not only to diet-induced hepatosteatosis, but also to increased lipoprotein-production induced by, e...
April 2018: Biochimie
Xiaoxue Zhang, Renpeng Ji, Huijun Sun, Jinyong Peng, Xiaodong Ma, ChangYuan Wang, Yufeng Fu, Liuchi Bao, Yue Jin
Nonalcoholic fatty liver disease (NAFLD) is characterised by excessive accumulation of hepatic lipids and oxidative injury of hepatocytes. Scutellarin is a flavonoid glycoside having antioxidative stress activity. Our current study aims to investigate the molecular mechanism of scutellarin ameliorating NAFLD. Scutellarin treatment was applied to male C57BL/6 mice maintained on a high-fat diet (HFD) and HepG2 cells challenged with oleic acid. The antioxidation biochemical indicators and lipid levels in the liver and cells were detected by kits...
February 2018: Free Radical Research
Lijun Pang, Kai Liu, Daojie Liu, Fudong Lv, Yunjin Zang, Fang Xie, Jiming Yin, Ying Shi, Yanjun Wang, Dexi Chen
Autophagy affects the pathological progression of non-alcoholic fatty liver disease (NAFLD); however, the precise role of autophagy in NAFLD remains unclear. In this study, we want to identify the role of autophagy including reticulophagy and mitophagy in NAFLD pathogenesis. When HepG2 cells were treated with 400 μM oleic acid (OA), increased reticulophagy was induced 8 h after treatment, which correlated with an anti-apoptotic response as shown by the activation of the PI3K/AKT pathway, an increase in BCL-2 expression, and the downregulation of OA-induced lipotoxicity...
January 24, 2018: Cell Death & Disease
Jingqing Chen, Xiaoshi Ma, Ying Yang, Zhaolai Dai, Zhenlong Wu, Guoyao Wu
Glycine supplementation has been reported to enhance white-fat loss and improve sensitivity to insulin in animals with obesity or type 2 diabetes. However, the underlying mechanisms responsible for the beneficial effects of glycine remain largely unknown. The purpose of this study was to test the hypothesis that glycine regulates adipocyte differentiation, adipogenesis, and lipolysis, therefore, contributing to white-fat reduction. 3T3-L1 pre-adipocytes were induced to differentiate into adipocytes in the presence of glycine (0, 0...
May 2018: Amino Acids
Guihong Yu, Shuai Wang, Lu Wang, Qian Che, Tianjiao Zhu, Guojian Zhang, Qianqun Gu, Peng Guo, Dehai Li
Seven new polyketides, named tanzawaic acids R-X ( 1 - 6 , 11 ), along with seven known analogues ( 7 - 10 and 12 - 14 ), were isolated from Penicillium steckii HDN13-279. Their structures, including the absolute configurations, were elucidated by NMR, MS, X-ray diffraction, circular dichroism (CD) analyses and chemical derivatization. Five compounds ( 2 , 3 , 6 , 10 and 12 ) significantly decreased the oleic acid (OA)-elicited lipid accumulation in HepG2 liver cells at the concentration of 10 μM, among which, four compounds ( 3 , 6 , 10 and 12 ) significantly decreased intracellular total cholesterol (TC) levels and three Compounds ( 3 , 6 , and 10 ) significantly decreased intracellular triglyceride (TG) levels...
January 12, 2018: Marine Drugs
Hiroe Go, Jin Ah Ryuk, Joo Tae Hwang, Byoung Seob Ko
Background: Gamisoyosan (GSS) is an herbal formula which has been used to treat women's diseases for several hundred years in Korea. GSS is one of the three most common prescriptions among women and is used to treat menopausal symptoms. Fatty liver disease is also common in postmenopausal women and can precede more severe diseases, such as steatohepatitis. The present study compared the effects of GSS on fatty liver using three different formulae, Dongui-Bogam (KIOM A), Korean Pharmacopeia (KIOM B) and Korean National Health Insurance (KIOM C)...
December 2017: Integrative Medicine Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"