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Gadolinium npo

Wei Zhang, Liu-Min Fan
Free cytosolic Ca(2+) ([Ca(2+)](cyt)) is an ubiquitous second messenger in plant cell signaling, and [Ca(2+)](cyt) elevation is associated with Ca(2+)-permeable channels in the plasma membrane and endomembranes regulated by a wide range of stimuli. However, knowledge regarding Ca(2+) channels and their regulation remains limited in planta. A type of voltage-dependent Ca(2+)-permeable channel was identified and characterized for the Vicia faba L. guard cell plasma membrane by using patch-clamp techniques. These channels are permeable to both Ba(2+) and Ca(2+), and their activities can be inhibited by micromolar Gd(3+)...
October 2009: Journal of Integrative Plant Biology
M C Wellner, G Isenberg
1. Stretch-activated channels (SACs) were analysed on patches attached to myocytes isolated from the guinea-pig urinary bladder. At 22 degrees C application of -2 to -4 kPa to the patch electrode induced SACs at a density of one to two per patch (3-5 M omega electrodes). 2. With electrodes containing 145 mM K+, 20 mM TEA and 2 mM Mg2+, the single channel current followed a linear I-V curve with a slope conductance of 39 +/- 5 pS (mean +/- S.D.) and a reversal potential of 2 +/- 6 mV. Substitution of chloride by aspartate ions left both parameters unchanged suggesting that the anions do not contribute to the currents...
July 1993: Journal of Physiology
T Nonaka, K Matsuzaki, K Kawahara, K Suzuki, M Hoshino
Ion channels in the apical membrane of rat inner medullary collecting duct (IMCD) were investigated by the patch clamp technique. Owing to the histological heterogeneity of IMCD, cells were cultured from the lower half of the inner medulla of Wistar rat kidney. Channel activity was rarely seen in cell attached patch, but membrane excision activated multiple units of 28.2 +/- 0.7 pS cation selective channel. A Na or K selective channel was not found. The 28 pS channel showed membrane voltage dependency, no rectification, almost equal permeability to monovalent cations (Na/K/Li/Cs/Rb/NH4 = 1:1...
February 15, 1995: Biochimica et Biophysica Acta
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