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https://www.readbyqxmd.com/read/28377637/in-vitro-antibacterial-activity-of-mgdg-palmitoyl-from-oscillatoria-acuminata-ntapc05-against-extended-spectrum-%C3%AE-lactamase-producers
#1
Abdul Azees Parveez Ahamed, Mohammed Uddin Rasheed, Kalilurrahuman Peer Muhamed Noorani, Nazar Reehana, Subramanian Santhoshkumar, Yousuff Mohamed Mohamed Imran, Naiyf S Alharbi, Chinnathambi Arunachalam, Sulaiman Ali Alharbi, Mohammad Abdulkader Akbarsha, Nooruddin Thajuddin
Extended-spectrum β-lactamase (ESBL)-producing bacteria pose a big challenge in clinical practices, warranting a new therapeutic strategy. In this study, methanol extract of the marine cyanobacterium Oscillatoria acuminata NTAPC05 was fractionated under bioassay guidance and the fractions were tested against three well-characterized ESBL-producing bacteria Escherichia coli U655, Stenotrophomonas maltophilia B929 and Enterobacter asburiae B938. Out of the four HPLC fractions, fraction 2 showed bactericidal activity against all the three ESBL producers much more efficiently (MIC 100 μg ml(-1)) than the fourth-generation cephalosporin (MIC >125 μg ml(-1))...
April 5, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/28374901/the-impact-of-carbapenem-resistance-on-clinical-deterioration-and-mortality-in-patients-with-liver-disease
#2
Philip G Ferstl, Natalie Filmann, Christian Brandt, Stefan Zeuzem, Michael Hogardt, Volkhard A J Kempf, Mona Müller, Oliver Waidmann, Claudia Reinheimer
BACKGROUND & AIMS: Infections with multidrug-resistant gram-negative bacteria are significantly impairing the prognosis of patients with liver disease. In particular, carbapenem resistance further narrows therapeutic options. This study investigates the impact of carbapenem-resistant gram-negative bacteria on the outcome of patients with liver disease and cirrhosis. METHODS: Between January 2011 and July 2015, 132 patients treated at the tertiary liver transplant center at University Hospital Frankfurt, Germany, were tested positive for carbapenem-resistant gram-negative bacteria and retrospectively analyzed in this study...
April 4, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28367030/clinical-characteristics-and-prognostic-factors-of-patients-with-stenotrophomonas-maltophilia-infections
#3
Priyam Batra, Purva Mathur, Mahesh C Misra
INTRODUCTION: Stenotrophomonas maltophilia earlier had limited pathogenic potential, but now with growing degree of immunosuppression in general population, it is being recognized as an important nosocomial pathogen. METHODOLOGY: A retrospective 7 years study was carried out to determine the clinical characteristics of all patients with Stenotrophomonas infections, antibiotic resistance pattern, and risk factors associated with hospital mortality. All patients with Stenotrophomonas culture positivity were identified and their medical records were reviewed...
April 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28358745/stenotrophomonas-maltophilia-endophthalmitis-1-month-after-intravitreal-aflibercept
#4
Paul S Boeke, Justin L Gottlieb
PURPOSE: Endophthalmitis caused by Stenotrophomonas maltophilia is rare and has been described after cataract surgery and open globe injuries. We report a patient with endophthalmitis caused by this organism after uncomplicated intravitreal aflibercept injection. METHODS: A 70-year-old man with a history of anti-vascular endothelial growth factor therapy for diabetic macular edema presented 23 days after aflibercept injection with clinical diagnosis of endophthalmitis...
March 29, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28341098/antimicrobial-activity-of-tigecycline-and-cefoperazone-sulbactam-tested-against-18-386-gram-negative-organisms-from-europe-and-the-asia-pacific-region-2013-2014
#5
M A Pfaller, R K Flamm, L R Duncan, R E Mendes, R N Jones, H S Sader
A total of 18,386 organisms, including 13,224 Enterobacteriaceae, 3536 Pseudomonas aeruginosa, 1254 Acinetobacter spp., and 372Stenotrophomonas maltophilia were collected from Western Europe (WEU; n=10,021), Eastern Europe (EEU; n=4957), and the Asia-Pacific region (APAC; n=3408 [1052 from China]) in 2013-2014 as part of the SENTRY Antimicrobial Surveillance Program and tested by a reference broth microdilution method for susceptibility against tigecycline, cefoperazone/sulbactam, and comparator agents. Overall, 95...
March 6, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28330112/feather-degradation-potential-of-stenotrophomonas-maltophilia-kb13-and-feather-protein-hydrolysate-fph-mediated-reduction-of-hexavalent-chromium
#6
Khushboo Bhange, Venkatesh Chaturvedi, Renu Bhatt
An efficient keratinolytic strain of Stenorophomonas maltophilia KB13 was isolated from feather disposal site of Bilaspur, Chhattisgarh, India. The strain could metabolize 10 g/l chicken feathers as sole source of carbon and nitrogen. Soluble protein, amino acid, and cysteine content were found to be maximum (690.6 ± 8.7, 688.9 ± 9.12 and 21 ± 0.36 µg/ml, respectively) at late logarithmic phase of growth. Protease and keratinase activity reached its maximum level (103.26 ± 7.09 and 178.5 ± 9...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28318778/stenotrophomonas-maltophilia-healthcare-associated-infections-identification-of-two-main-pathogenic-genetic-backgrounds
#7
C Corlouer, B Lamy, M Desroches, J Ramos-Vivas, E Mehiri-Zghal, O Lemenand, J-M Delarbre, J-W Decousser
BACKGROUND: Stenotrophomonas maltophilia is an opportunistic multi-drug-resistant bacterium responsible for healthcare-associated infections. Strategies for in-hospital infection control and management of carriers and environmental reservoirs remain controversial. AIM: To determine the population structure of S. maltophilia strains in hospitalized infected patients and to identify putative highly pathogenic subpopulations that require upgraded infection control measures...
February 9, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28298316/paediatric-infectious-keratitis-a-case-series-of-107-children-presenting-to-a-tertiary-referral-centre
#8
Julia Dutra Rossetto, Kara M Cavuoto, Carla J Osigian, Ta Chen Peter Chang, Darlene Miller, Hilda Capo, Oriel Spierer
BACKGROUND/AIMS: Corneal ulcers can result in severe visual impairment in children. The recent trends of paediatric microbial ulcerative keratitis in the USA are unknown. The purpose of this study is to report the risk factors, microbiological profile and treatment outcomes of paediatric microbial keratitis in South Florida. METHODS: A university-based tertiary eye care centre retrospective case series between 1992 and 2015. Medical records of 107 paediatric patients (age <18 years) with the diagnosis of microbial ulcerative keratitis were analysed...
March 15, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28291834/the-isolation-and-characterization-of-stenotrophomonas-maltophilia-t4-like-bacteriophage-dlp6
#9
Danielle L Peters, Paul Stothard, Jonathan J Dennis
Increasing isolation of the extremely antibiotic resistant bacterium Stenotrophomonas maltophilia has caused alarm worldwide due to the limited treatment options available. A potential treatment option for fighting this bacterium is 'phage therapy', the clinical application of bacteriophages to selectively kill bacteria. Bacteriophage DLP6 (vB_SmoM-DLP6) was isolated from a soil sample using clinical isolate S. maltophilia strain D1571 as host. Host range analysis of phage DLP6 against 27 clinical S. maltophilia isolates shows successful infection and lysis in 13 of the 27 isolates tested...
2017: PloS One
https://www.readbyqxmd.com/read/28287494/synthetic-peptides-derived-from-bovine-lactoferricin-exhibit-antimicrobial-activity-against-e-coli-atcc-11775-s-maltophilia-atcc-13636-and-s-enteritidis-atcc-13076
#10
Nataly De Jesús Huertas Méndez, Yerly Vargas Casanova, Anyelith Katherine Gómez Chimbi, Edith Hernández, Aura Lucia Leal Castro, Javier Mauricio Melo Diaz, Zuly Jenny Rivera Monroy, Javier Eduardo García Castañeda
Linear, dimeric, tetrameric, and cyclic peptides derived from lactoferricin B-containing non-natural amino acids and the RWQWR motif were synthesized, purified, and characterized using RP-HPLC, MALDI-TOF mass spectrometry, and circular dichroism. The antibacterial activity of peptides against Escherichia coli ATCC 11775, Stenotrophomonas maltophilia ATCC 13636, and Salmonella enteritidis ATCC 13076 was evaluated. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined...
March 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28284526/association-between-glucose-intolerance-and-bacterial-colonisation-in-an-adult-population-with-cystic-fibrosis-emergence-of-stenotrophomonas-maltophilia
#11
C Lehoux Dubois, V Boudreau, F Tremblay, A Lavoie, Y Berthiaume, R Rabasa-Lhoret, A Coriati
BACKGROUND: Diabetes is common in cystic fibrosis (CF). Glucose can be detected in the airway when the blood glucose is elevated, which favours bacterial growth. We investigated the relationship between dysglycemia and lung pathogens in CF. METHODS: Cross-sectional and prospective analysis of CF patients (N=260) who underwent a 2h-oral glucose tolerance test. Clinical data was collected. RESULTS: Stenotrophomonas maltophilia (S. maltophilia) was the sole bacteria increased in dysglycemic (AGT: 20...
March 8, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28275097/simplified-and-representative-bacterial-community-of-maize-roots
#12
Ben Niu, Joseph Nathaniel Paulson, Xiaoqi Zheng, Roberto Kolter
Plant-associated microbes are important for the growth and health of their hosts. As a result of numerous prior studies, we know that host genotypes and abiotic factors influence the composition of plant microbiomes. However, the high complexity of these communities challenges detailed studies to define experimentally the mechanisms underlying the dynamics of community assembly and the beneficial effects of such microbiomes on plant hosts. In this work, from the distinctive microbiota assembled by maize roots, through host-mediated selection, we obtained a greatly simplified synthetic bacterial community consisting of seven strains (Enterobacter cloacae, Stenotrophomonas maltophilia, Ochrobactrum pituitosum, Herbaspirillum frisingense, Pseudomonas putida, Curtobacterium pusillum, and Chryseobacterium indologenes) representing three of the four most dominant phyla found in maize roots...
March 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28273966/stenotrophomonas-maltophilia-an-emerging-pathogen-in-paediatric-population
#13
Charu Nayyar, Preeti Thakur, Vibhor Tak, Karnika Saigal
INTRODUCTION: Stenotrophomonas maltophilia (formerly Pseudomonas maltophilia/Xanthomonas maltophilia), a Gram- negative, non-fermenting bacillus, is being increasingly recognized as a threatening nosocomial pathogen, associated with significant mortality. AIM: To determine the prevalence of infection, antimicrobial susceptibility pattern and clinical outcome of S. maltophilia in a paediatric population. MATERIALS AND METHODS: This was a retrospective study conducted over a period of eight months, i...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28273965/emergence-of-carbapenem-resistant-non-fermenting-gram-negative-bacilli-isolated-in-an-icu-of-a-tertiary-care-hospital
#14
Sonika Agarwal, Barnali Kakati, Sushant Khanduri, Shalini Gupta
INTRODUCTION: The emergence and spread of Multi-Drug Resistant (MDR) Non-Fermenting Gram-Negative Bacilli (NFGNB) in Intensive Care Units (ICU) and their genetic potential to transmit diverse antibiotic resistance regardless of their ability to ferment glucose poses a major threat in hospitals. The complex interplay of clonal spread, persistence, transmission of resistance elements and cell-cell interaction leads to the difficulty in controlling infections caused by these multi drug-resistant strains...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28264910/the-expanding-role-of-type-ii-secretion-in-bacterial-pathogenesis-and-beyond
#15
Nicholas P Cianciotto, Richard C White
Type II secretion (T2S) is one means by which Gram-negative pathogens secrete proteins into the extracellular milieu and/or host organisms. Based upon recent genome sequencing, it is clear that T2S is largely restricted to the Proteobacteria, occurring in many, but not all genera in the α-, β-, γ-, and δ- classes. Prominent human and/or animal pathogens that express a T2S system(s) include Acinetobacter baumannii, Burkholderia pseudomallei, Chlamydia trachomatis, Escherichia coli, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Vibrio cholerae, and Yersinia enterocolitica T2S-expressing plant pathogens include Dickeya dadantii, Erwinia amylovora, Pectobacterium carotovorum, Ralstonia solanacearum, Xanthomonas campestris, Xanthomonas oryzae, and Xylella fastidiosa T2S also occurs in non-pathogenic bacteria, facilitating symbioses, among other things...
March 6, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28261845/stenotrophomonas-maltophilia-isolated-from-prostatic-fluid-as-an-infertility-factor-in-a-male-dog
#16
A Domosławska, S Zduńczyk, A Jurczak, T Janowski
The aim of this case was to describe very rare infection of canine prostate gland with Stenotrophomonas maltophilia which had influence on male fertility. The bacterium was cultured from third fraction of the ejaculate collected by manual manipulation. The sperm concentration and motility parameters were evaluated by Hamilton-Thorne Sperm Analyser, version IVOS 12.3, sperm morphology by Diff-Quick staining and live/dead spermatozoa by eosin/nigrosin staining. After 3 weeks of treatment with targeted antibiotic, ciprofloxacin, there was no bacterial growth in prostate fluid...
March 6, 2017: Andrologia
https://www.readbyqxmd.com/read/28257816/evaluation-of-trimethoprim-sulfamethoxazole-based-combination-therapy-against-stenotrophomonas-maltophilia-in-vitro-effects-and-clinical-efficacy-in-cancer-patients
#17
Hideki Araoka, Masaru Baba, Chikako Okada, Masahiro Abe, Muneyoshi Kimura, Akiko Yoneyama
OBJECTIVES: The aim of this study was to evaluate the in vitro effects and clinical efficacies of trimethoprim-sulfamethoxazole (SXT) combined with other antimicrobial agents against Stenotrophomonas maltophilia. METHODS: In vitro analysis was conducted on 89 S. maltophilia strains isolated from blood and the respiratory tract between June 2012 and October 2014. Levofloxacin (LVX), ticarcillin-clavulanic acid (TIM), and minocycline (MIN) were selected for an examination of their effects when individually combined with SXT by the checkerboard method...
February 28, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28251535/copper-tolerant-rhizosphere-bacteria-characterization-and-assessment-of-plant-growth-promoting-factors
#18
Manohari Rathi, Yogalakshmi Kadapakkam Nandabalan
Remediation of heavy metal contaminated soil is a major problem or concern worldwide. Heavy metal accumulation in the soil is increasing day by day by industries, mines, agriculture, fuel combustion and municipal waste discharge. Such contaminated soils harbour a large number of resistant microbial populations. Screening and isolation of such microbes would be utilized for natural remediation of metal contaminated soils. Therefore, in the present study, highly copper-tolerant bacteria from rhizosphere soil of Cynodon dactylon grown in brass effluent contaminated soil were isolated and assessed for plant growth promoting factors...
March 1, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28235876/comparison-of-biochemical-properties-of-the-original-and-newly-identified-oleate-hydratases-from-stenotrophomonas-maltophilia
#19
Woo-Ri Kang, Min-Ju Seo, Kyung-Chul Shin, Jin-Byung Park, Deok-Kun Oh
Oleate hydratases (OhyAs) catalyze the conversion of unsaturated fatty acids to 10-hydroxy fatty acids, which are used as precursors of important industrial compounds, including lactones, and ω-hydroxycarboxylic and α,ω-dicarboxylic acids. The genes encoding OhyA and a putative fatty acid hydratase in Stenotrophomonas maltophilia were identified by genomic analysis. The putative fatty acid hydratase was purified and identified as an oleate hydratase (OhyA2) based on its substrate specificity. The activity of OhyA2 as a holoenzyme was not affected on adding cofactors, whereas the activity of the original oleate hydratase (OhyA1) showed an increase...
February 24, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28223380/vitamin-k3-induces-the-expression-of-the-stenotrophomonas-maltophilia-smevwx-multidrug-efflux-pump
#20
P Blanco, F Corona, M B Sánchez, J L Martínez
Stenotrophomonas maltophilia is an opportunistic pathogen, with increasing prevalence, able to cause infections in immunocompromised patients or in those with a previous pathology. The treatment of the infections caused by this bacterium is often complicated due to the several intrinsic antibiotic resistance mechanisms that it presents. Multidrug efflux pumps are among the best studied mechanisms of S. maltophilia antibiotic resistance. Some of these efflux pumps have a basal expression level but, in general, their expression is often low and only reaches high levels when the local regulator is mutated or bacteria are in the presence of an effector...
February 21, 2017: Antimicrobial Agents and Chemotherapy
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