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listeria guidelines

Manan Sharma, Russell Reynnells
Biological soil amendments (BSAs) such as manure and compost are frequently used as organic fertilizers to improve the physical and chemical properties of soils. However, BSAs have been known to be a reservoir for enteric bacterial pathogens such as enterohemorrhagic Escherichia coli (EHEC), Salmonella spp., and Listeria spp. There are numerous mechanisms by which manure may transfer pathogens to growing fruits and vegetables, and several outbreaks of infections have been linked to manure-related contamination of leafy greens...
August 2016: Microbiology Spectrum
K Rietberg, J Lloyd, B Melius, P Wyman, R Treadwell, G Olson, M-G Kang, J S Duchin
Two cases of hospital-acquired listeriosis were linked to a commercially produced, pasteurized ice cream mix. Manufacturers should implement safety measures from the Food Safety Modernization Act to minimize the risk of Listeria contamination. Dietary guidelines for persons at high risk of listeriosis may need revision to recognize the potential risk from pasteurized products.
October 2016: Epidemiology and Infection
Patrick Bird, Jonathan Flannery, Erin Crowley, James Agin, David Goins, Lisa Monteroso, DeAnn Benesh
The 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes combines isothermal amplification and bioluminescence to detect Listeria monocytogenes with high specificity and efficiency in select foods and environmental samples. The 3M MDA Listeria monocytogenes method was evaluated using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook 8.09 (2011) Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products and Environmental Samples for deli turkey, and the AOAC Official Method of Analysis(SM) 993...
July 2015: Journal of AOAC International
Swapnil Doijad, Markus Weigel, Sukhadeo Barbuddhe, Jochen Blom, Alexander Goesmann, Torsten Hain, Trinad Chakraborty
The precise delineation of lineages and clonal groups are a prerequisite to examine within-species genetic variations, particularly with respect to pathogenic potential. A whole-genome-based approach was used to subtype and subgroup isolates of Listeria monocytogenes. Core-genome typing was performed, employing 3 different approaches: total core genes (CG), high-scoring segment pairs (HSPs), and average nucleotide identity (ANI). Examination of 113 L. monocytogenes genomes available in-house and in public domains revealed 33 phylogenomic groups (PGs)...
September 2015: Canadian Journal of Microbiology
Adisorn Swetwiwathana, Wonnop Visessanguan
Lactic acid bacteria (LAB) are very important in converting of agricultural products into safe, delicious and shelf stable foods for human consumption. The preservative activity of LAB in foods is mainly attributed to the production of anti-microbial metabolites such as organic acids and bacteriocins which enables them to grow and control the growth of pathogens and spoilage microorganisms. Besides ensuring safety, bacteriocin-producing LAB with their probiotic potentials could also be emerging as a means to develop functional meat products with desirable health benefits...
November 2015: Meat Science
Erika M du Plessis, Francois Duvenage, Lise Korsten
The potential transfer of human pathogenic bacteria present in irrigation water onto fresh produce was investigated, because surface water sources used for irrigation purposes in South Africa have increasingly been reported to be contaminated with enteric bacterial pathogens. A microbiological analysis was performed of a selected river in Limpopo Province, South Africa, that is often contaminated with raw sewage from municipal sewage works and overhead irrigated onions produced on a commercial farm. Counts of Escherichia coli, coliforms, aerobic bacteria, fungi, and yeasts and the prevalence of E...
April 2015: Journal of Food Protection
Marilyn C Erickson, Chris Smith, Xiuping Jiang, Ian D Flitcroft, Michael P Doyle
Heat is the primary mechanism by which aerobic composting inactivates zoonotic bacterial pathogens residing within animal manures, but at sublethal temperatures, the time necessary to hold the compost materials to ensure pathogen inactivation is uncertain. To determine the influence of the type of nitrogen amendment on inactivation of Salmonella, Listeria monocytogenes, and Escherichia coli O157:H7 in compost mixtures stored at sublethal temperatures, specific variables investigated in these studies included the animal source of the manure, the initial carbon/nitrogen (C:N) ratio of the compost mixture, and the age of the manure...
February 2015: Journal of Food Protection
Eun Sook Choi, Nam Hee Kim, Hye Won Kim, Sun Ae Kim, Jun Il Jo, Soon Han Kim, Soon Ho Lee, Sang Do Ha, Min Suk Rhee
Microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. Laver or supplements were collected from six manufacturers (A to F) to assess potential microbiological hazards and the critical control points in commercial processing lines. Aerobic plate counts (APC), coliform counts, Bacillus cereus, Staphylococcus aureus, and Vibrio parahaemolyticus were enumerated, and the presence of B. cereus, Listeria monocytogenes, Salmonella, S. aureus, and V. parahaemolyticus were confirmed during processing...
December 2014: Journal of Food Protection
L Vakrilova, St Hitrova, E Shopova, R Rousseva, A Radulova, A Masseva
UNLABELLED: Improved obstetrical management and evidence-based peripartum antimicrobial therapy in the last decades reduce the frequency of early-onset neonatal sepsis (EONS) and improve the outcome. The spectrum of the microrganisms is different according to regions, countries and periods of time. Appropriate diagnosis and treatment improve the outcome. However dissociation often occurs between clinical manifestation, laboratory and microbiological findings. AIMS: To determine the incidence of neonatal bacteremia in our hospital, to identify the spectrum of organisms from positive blood cultures (BC), to analyze the correlation between clinical manifestation of EOS and/or laboratory abnormalities...
2014: Akusherstvo i Ginekologii︠a︡
Amy M Dechet, Karen M Herman, Cary Chen Parker, Peter Taormina, Joy Johanson, Robert V Tauxe, Barbara E Mahon
After a series of outbreaks associated with sprouts in the mid-1990s, the U.S. Food and Drug Administration (FDA) published guidelines in 1999 for sprouts producers to reduce the risk of contamination. The recommendations included treating seeds with an antimicrobial agent such as calcium hypochlorite solution and testing spent irrigation water for pathogens. From 1998 through 2010, 33 outbreaks from seed and bean sprouts were documented in the United States, affecting 1330 reported persons. Twenty-eight outbreaks were caused by Salmonella, four by Shiga toxin-producing Escherichia coli, and one by Listeria...
August 2014: Foodborne Pathogens and Disease
Ifeanyichukwu O Okike, Alan P Johnson, Katherine L Henderson, Ruth M Blackburn, Berit Muller-Pebody, Shamez N Ladhani, Mark Anthony, Nelly Ninis, Paul T Heath
BACKGROUND: Bacterial meningitis remains a major cause of morbidity and mortality in young infants. Understanding the epidemiology and burden of disease is important. METHODS: Prospective, enhanced, national population-based active surveillance was undertaken to determine the incidence, etiology, and outcome of bacterial meningitis in infants aged <90 days in the United Kingdom and Ireland. RESULTS: During July 2010-July 2011, 364 cases were identified (annual incidence, 0...
November 15, 2014: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Erin Crowley, Patrick Bird, Jonathan Flannery, M Joseph Benzinger, Kiel Fisher, Megan Boyle, Travis Huffman, Ben Bastin, Paige Bedinghaus, William Judd, Thao Hoang, James Agin, David Goins, Ronald L Johnson
The VIDAS Listeria monocytogenes Xpress (LMX) is an automated rapid screening enzyme immunoassay for the detection of Listeria monocytogenes in food products. The VIDAS LMX method was compared in a multi-laboratory collaborative study to AOAC Official Method 993.12 Listeria monocytogenes in Milk and Dairy Products reference method following current AOAC guidelines. A total of 14 laboratories participated, representing government and industry, throughout the United States. One matrix, queso fresco (soft Mexican cheese), was analyzed using two different test portion sizes, 25 and 125 g...
March 2014: Journal of AOAC International
Erin Crowley, Patrick Bird, Jonathan Flannery, M Joseph Benzinger, Kiel Fisher, Megan Boyle, Travis Huffman, Ben Bastin, Paige Bedinghaus, William Judd, Thao Hoang, James Agin, David Goins, Ronald L Johnson
The VIDAS UP Listeria (LPT) is an automated rapid screening enzyme phage-ligand based assay for the detection of Listeria species in human food products and environmental samples. The VIDAS LPT method was compared in a multi-laboratory collaborative study to AOAC Official Method 993.12 Listeria monocytogenes in Milk and Dairy Products reference method following current AOAC guidelines. A total of 14 laboratories participated, representing government and industry, throughout the United States. One matrix, queso fresco (soft Mexican cheese), was analyzed using two different test portion sizes, 25 and 125 g...
March 2014: Journal of AOAC International
Habib Bostan, Yakup Tomak, Sengul Alpay Karaoglu, Basar Erdivanli, Volkan Hanci
BACKGROUND: Drugs administered as intravenous infusion may be contaminated during several stages of production or preparation. However studies focusing on antibacterial effects of vasopressor drugs are very rare. This study investigates the in vitro antimicrobial activity of the clinically used forms of vasopressors. MATERIALS AND METHODS: In vitro antimicrobial activities of vasopressor drugs of different concentrations were investigated by using the micro dilution technique...
March 2014: Brazilian Journal of Anesthesiology
Jiang Jiang, Youling L Xiong
Ready-to-eat (RTE) muscle foods refer to a general category of meat and poultry products that are fully cooked and consumable without reheating. These products, including whole and sliced pork, beef, turkey, chicken, and variety of meats, in the forms of ham, roast, rolls, sausage, and frankfurter, are widely available in the delicatessen section of retail stores or various food service outlets. However, difficulties in avoidance of contamination by foodborne pathogens, notably Listeria monocytogenes, during product postlethality repackaging render RTE meats labile to outbreaks...
2015: Critical Reviews in Food Science and Nutrition
Moira Madeo, Rosario Musumeci, Anna Maria Laura Careddu, Ettore Amato, Mirella Maria Pontello, Clementina Elvezia Cocuzza
Susceptibility of 96 Listeria monocytogenes human isolates collected in northern Italy between 2008 and 2010, to 15 antimicrobials, was investigated. Minimum inhibitory concentration (MIC) was evaluated by means of the standardized broth microdilution method, according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) international guidelines. All L. monocytogenes human isolates were susceptible to penicillin G (MIC(90)≤0·06 μg/ml), meropenem (MIC(90)≤0·06 μg/ml), and erythromycin (MIC(90) = 0·12 μg/ml)...
August 2015: Journal of Chemotherapy
Mark S Rundell, Maneesh Pingle, Sanchita Das, Aashiq Hussain, Oksana Ocheretina, Macarthur Charles, Davise H Larone, Eric D Spitzer, Linnie Golightly, Francis Barany
Enteric pathogens that cause gastroenteritis remain a major global health concern. The goal of this study was to develop a multiplex PCR/ligation detection reaction (LDR) assay for the detection of all NIAID category B bacterial food and water-borne pathogens directly from stool specimens. To validate the PCR/LDR assay, clinical isolates of Campylobacter spp., Vibrio spp., Shigella spp., Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and diarrheagenic Escherichia coli were tested. The sensitivity and specificity of the assay were assessed using a large number of seeded culture-negative stool specimens and a smaller set of clinical specimens from Haiti...
June 2014: Diagnostic Microbiology and Infectious Disease
Ameer Hassoun, Curt Stankovic, Alexander Rogers, Elizabeth Duffy, Marwan Zidan, Clare Levijoki, Rachel Stanley, Prashant Mahajan
INTRODUCTION: Empiric parenteral ampicillin has traditionally been used to treat listeria and enterococcal serious bacterial infections (SBI) in neonates 28 days of age or younger. Anecdotal experience suggests that these infections are rare. Existing data suggest an increasing resistance to ampicillin. Guidelines advocating the routine use of empiric ampicillin may need to be revisited. OBJECTIVE: This study aimed to describe the epidemiology and ampicillin sensitivity of listeria and enterococcal infections in neonates 28 days of age and younger who presented to 2 pediatric emergency departments (ED) in Michigan...
April 2014: Pediatric Emergency Care
David Bertsch, Mirjam Muelli, Monika Weller, Anaïs Uruty, Christophe Lacroix, Leo Meile
The aims of this study were to assess antibiotic resistance pheno- and genotypes in foodborne, clinical, and environmental Listeria isolates, as well as to elucidate the horizontal gene transfer potential of detected resistance genes. A small fraction of in total 524 Listeria spp. isolates (3.1%) displayed acquired antibiotic resistance mainly to tetracycline (n = 11), but also to clindamycin (n = 4) and trimethoprim (n = 3), which was genotypically confirmed. In two cases, a tetracycline resistance phenotype was observed together with a trimethoprim resistance phenotype, namely in a clinical L...
February 2014: MicrobiologyOpen
Béatrice Marquez, Vincent Pourcelle, Coralie M Vallet, Marie-Paule Mingeot-Leclercq, Paul M Tulkens, Jacqueline Marchand-Bruynaert, Françoise Van Bambeke
PURPOSE: To evaluate pharmacological properties (antibacterial activity; accumulation in phagocytic cells; activity against intracellular bacteria; susceptibility to fluoroquinolone efflux transporters) of ciprofloxacin derivatives modified at C-7 of the piperazine ring. METHODS: N-acetyl- (1), N-benzoyl- (2), N-ethyl- (3), and N-benzyl- (4) ciprofloxacin were synthesized. MICs against Escherichia coli and Staphylococcus aureus were determined following CLSI guidelines...
May 2014: Pharmaceutical Research
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