Read by QxMD icon Read


Stuart J Grice, James N Sleigh, M Zameel Cader
Dominant mutations in GARS , encoding the ubiquitous enzyme glycyl-tRNA synthetase (GlyRS), cause peripheral nerve degeneration and Charcot-Marie-Tooth disease type 2D (CMT2D). This genetic disorder exemplifies a recurring paradigm in neurodegeneration, in which mutations in essential genes cause selective degeneration of the nervous system. Recent evidence suggests that the mechanism underlying CMT2D involves extracellular neomorphic binding of mutant GlyRS to neuronally-expressed proteins. Consistent with this, our previous studies indicate a non-cell autonomous mechanism, whereby mutant GlyRS is secreted and interacts with the neuromuscular junction (NMJ)...
2018: Frontiers in Molecular Neuroscience
Kyle A Grice, Graham Bailey Griffin, Phoebus Sun Cao, Cesar Saucedo, Aeshah H Niyazi, Fatimah Aldakheel, George E Sterbinsky, Robert J LeSuer
The solution-phase structure and electronic relaxation dynamics of zinc bis-8-hydroxyquinoline [Zn(8HQ)2 ] in dimethylsulfoxide (DMSO) were examined using a broad array of spectroscopic techniques, complimented by ab initio calculations of molecular structure. The ground-state structure was determined using Extended X-Ray Absorption Fine Structure (EXAFS) data collected on the Zn K-edge and DOSY NMR spectroscopy. The complex was found to be monomeric and octahedral, with two bidentate 8-hydroxyquinolate ligands and two DMSO molecules coordinated to the zinc through oxygen atoms...
March 6, 2018: Journal of Physical Chemistry. A
Alexey S Rzhevskiy, Krishna Telaprolu, Yousuf H Mohammed, Jeffrey E Grice, Michael S Roberts, Yuri G Anissimov
The mathematical model describing drug flux through microporated skin was previously developed. Based on this model, two mathematical equations can be used to predict the microporatio-enhanced transdermal drug flux: the complex primal equation containing a variety of experimentally-determined variables, and the simplified straightforward equation. In this study, experimental transdermal fluxes of three corticosteroids through split-thickness human skin treated with a microneedle roller were measured, and the values of fluxes compared with those predicted using both the more complex and simplified equations...
January 31, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Shannon D Dehghanpir, Claire H Birkenheuer, Kui Yang, Ryan P Murelli, Lynda A Morrison, Stuart F J Le Grice, Joel D Baines
Herpesviruses are ubiquitous in animals and cause economic losses concomitant with many diseases. Most of the domestic animal herpesviruses are within the subfamily Alphaherpesvirinae, which includes human herpes simplex virus 1 (HSV-1). Suppression of HSV-1 replication has been reported with α-hydroxytropolones (αHTs), aromatic ring compounds that have broad bioactivity due to potent chelating activity. It is postulated that αHTs inhibit enzymes within the nucleotidyltransferase superfamily (NTS). These enzymes require divalent cations for nucleic acid cleavage activity...
February 2018: Veterinary Microbiology
Antonina J Kruppa, Chieko Kishi-Itakura, Thomas A Masters, Joanna E Rorbach, Guinevere L Grice, John Kendrick-Jones, James A Nathan, Michal Minczuk, Folma Buss
Mitochondrial quality control is essential to maintain cellular homeostasis and is achieved by removing damaged, ubiquitinated mitochondria via Parkin-mediated mitophagy. Here, we demonstrate that MYO6 (myosin VI), a unique myosin that moves toward the minus end of actin filaments, forms a complex with Parkin and is selectively recruited to damaged mitochondria via its ubiquitin-binding domain. This myosin motor initiates the assembly of F-actin cages to encapsulate damaged mitochondria by forming a physical barrier that prevents refusion with neighboring populations...
January 31, 2018: Developmental Cell
Jacquelyn S Meisel, Georgia Sfyroera, Casey Bartow-McKenney, Ciara Gimblet, Julia Bugayev, Joseph Horwinski, Brian Kim, Jonathan R Brestoff, Amanda S Tyldsley, Qi Zheng, Brendan P Hodkinson, David Artis, Elizabeth A Grice
BACKGROUND: The skin harbors complex communities of resident microorganisms, yet little is known of their physiological roles and the molecular mechanisms that mediate cutaneous host-microbe interactions. Here, we profiled skin transcriptomes of mice reared in the presence and absence of microbiota to elucidate the range of pathways and functions modulated in the skin by the microbiota. RESULTS: A total of 2820 genes were differentially regulated in response to microbial colonization and were enriched in gene ontology (GO) terms related to the host-immune response and epidermal differentiation...
January 30, 2018: Microbiome
Eman Abd, Heather A E Benson, Michael S Roberts, Jeffrey E Grice
In this work, we examined enhanced skin delivery of minoxidil applied in nanoemulsions incorporating skin penetration enhancers. Aliquots of fully characterized oil-in-water nanoemulsions (1 mL), containing minoxidil (2%) and the skin penetration enhancer oleic acid or eucalyptol as oil phases, were applied to full-thickness excised human skin in Franz diffusion cells, while aqueous solutions (1 mL) containing minoxidil were used as controls. Minoxidil in the stratum corneum (SC), hair follicles, deeper skin layers, and flux through the skin over 24 h was determined, as well as minoxidil solubility in the formulations and in the SC...
January 25, 2018: Pharmaceutics
Doris Mücke, Anne Hermes, Timo B Roettger, Johannes Becker, Henrik Niemann, Till A Dembek, Lars Timmermann, Veerle Visser-Vandewalle, Gereon R Fink, Martine Grice, Michael T Barbe
Acoustic studies have revealed that patients with Essential Tremor treated with thalamic Deep Brain Stimulation (DBS) may suffer from speech deterioration in terms of imprecise oral articulation and reduced voicing control. Based on the acoustic signal one cannot infer, however, whether this deterioration is due to a general slowing down of the speech motor system (e.g., a target undershoot of a desired articulatory goal resulting from being too slow) or disturbed coordination (e.g., a target undershoot caused by problems with the relative phasing of articulatory movements)...
2018: PloS One
Jade Le Grice, Virginia Braun
Indigenous (Māori) psychologies of sexual health occur at the cultural nexus of Indigenous and Western knowledge, colonising influence and intervention. Formal school-based sexuality education holds potential to intervene in this psychological space by decolonising notions of Māori sexuality, relationships and reproduction. This research utilises an Indigenous feminist (Mana Wāhine) methodology and interviews with 43 Māori participants (26 women and 17 men). We explore how Māori knowledges (mātauranga Māori), responsive to the surrounding colonising context, were interwoven through four themes: relationships, reproductive responsibility, open conversations about sexuality and contraceptive education...
November 1, 2017: Journal of Health Psychology
S M Neamul Kabir Zihad, Sanjib Saha, Md Sifujjaman Rony, Hasna Banu, Shaikh J Uddin, Jamil A Shilpi, I Darren Grice
ETHNOBOTANICAL RELEVANCE: Bambusa arundinacea (Retz.) Willd., commonly known as Kanta Bans, plays an important ethnobotanical role, especially in Asia. In traditional medicine it has reportedly been used for the treatment of constipation, blood-diseases, leucodema, inflammation and urinary discharges. A number of ethnomedicinal records exist regarding the use of B. arundinaceae shoots as a laxative, however, there are no scientific studies reported on its laxative activity. Therefore, the aim of this present study was to evaluate the laxative activity of an ethanolic B...
March 25, 2018: Journal of Ethnopharmacology
Gloria R Grice, Nicole M Gattas, Theresa Prosser, Mychal Voorhees, Clark Kebodeaux, Amy Tiemeier, Tricia M Berry, Alexandria Garavaglia Wilson, Janelle Mann, Paul Juang
Objective. To develop a comprehensive instrument specific to student pharmacist-patient communication skills, and to determine face, content, construct, concurrent, and predictive validity and reliability of the instrument. Methods. A multi-step approach was used to create and validate an instrument, including the use of external experts for face and content validity, students for construct validity, comparisons to other rubrics for concurrent validity, comparisons to other coursework for predictive validity, and extensive reliability and inter-rater reliability testing with trained faculty assessors...
October 2017: American Journal of Pharmaceutical Education
Youping Zhou, Benli Zhang, Hilary Stuart-Williams, Kliti Grice, Charles H Hocart, Arthur Gessler, Zachary E Kayler, Graham D Farquhar
Compartmentation of C4 photosynthetic biochemistry into bundle sheath (BS) and mesophyll (M) cells, and photorespiration in C3 plants is predicted to have hydrogen isotopic consequences for metabolites at both molecular and site-specific levels. Molecular-level evidence was recently reported (Zhou et al., 2016), but evidence at the site-specific level is still lacking. We propose that such evidence exists in the contrasting2 H distribution profiles of glucose samples from naturally grown C3 , C4 and CAM plants: photorespiration contributes to the relative2 H enrichment in H5 and relative2 H depletion in H1 & H6 (the average of the two pro-chiral Hs and in particular H6, pro-R ) in C3 glucose, while2 H-enriched C3 mesophyll cellular (chloroplastic) water most likely contributes to the enrichment at H4 ; export of (transferable hydrogen atoms of) NADPH from C4 mesophyll cells to bundle sheath cells (via the malate shuttle) and incorporation of2 H-relatively unenriched BS cellular water contribute to the relative depletion of H4 & H5 respectively; shuttling of triose-phosphates (PGA: phosphoglycerate dand DHAP: dihydroacetone phosphate) between C4 bundle sheath and mesophyll cells contributes to the relative enrichment in H1 & H6 (in particular H6, pro-R ) in C4 glucose...
January 2018: Phytochemistry
Zhimin Sha, Qingnan Chu, Zheng Zhao, Yubo Yue, Linfang Lu, Jing Yuan, Linkui Cao
Introducing frogs into paddy fields can control pests and diseases, and organic farming can improve soil fertility and rice growth. The aim of this 2-year field study was compare the yield and elemental composition of rice between an organic farming system including frogs (ORF) and a conventional rice culture system (CR). The grain yields were almost the same in the ORF system and the CR system. The ORF significantly increased the contents of phosphorus (P), ion (Fe), zinc (Zn), molybdenum (Mo) and selenium (Se) in rice grain at one or both years...
November 16, 2017: Scientific Reports
Elizabeth A Grice, Thomas L Dawson
The skin is our first line of defense, protecting us from invasion and evaporation. Its variable structure, changing geography, and complex immune repertoire provide a vast interface for our cutaneous microbial community. Skin is inhabited by many thousands of microbes, but this review focuses on the dominant eukaryote, Malassezia, and its host interaction. Malassezia compromises 17 species with variable niche specificities and differing pathogenic potential. It has been known as a skin inhabitant for over 100 years, and is now accepted to be on all warm-blooded animals...
November 12, 2017: Current Opinion in Microbiology
Newton Andréo-Filho, Antonio Vinicius Kosiski Bim, Telma Mary Kaneko, Nidia Ayumi Kitice, Isha N Haridass, Eman Abd, Patricia Santos Lopes, Sachin S Thakur, Harendra S Parekh, Michael S Roberts, Jeffrey E Grice, Heather A E Benson, Vânia Rodrigues Leite-Silva
The use of sunscreen products is widely promoted by schools, government agencies, and health-related organizations to minimize sunburn and skin damage. In this study, we developed stable solid lipid nanoparticles (SLNs) containing the chemical UV filter octyl methoxycinnamate (OMC). In parallel, we produced similar stable SLNs in which 20% of the OMC content was replaced by the botanical urucum oil. When these SLNs were applied to the skin of human volunteers, no changes in fluorescence lifetimes or redox ratios of the endogenous skin fluorophores were seen, suggesting that the formulations did not induce toxic responses in the skin...
November 4, 2017: Skin Pharmacology and Physiology
Thomas Grice-Jackson, Hugo D Critchley, Michael J Banissy, Jamie Ward
Around a quarter of the population report "mirror pain" experiences in which bodily sensations of pain are elicited in response to viewing another person in pain. We have shown that this population of responders further fractionates into two distinct subsets (Sensory/localized and Affective/General), which presents an important opportunity to investigate the neural underpinnings of individual differences in empathic responses. Our study uses fMRI to determine how regions involved in the perception of pain interact with regions implicated in empathic regulation in these two groups, relative to controls...
2017: Frontiers in Human Neuroscience
Benjamin Hon Kei Yip, Dan Bai, Behrang Mahjani, Lambertus Klei, Yudi Pawitan, Christina M Hultman, Dorothy E Grice, Kathryn Roeder, Joseph D Buxbaum, Bernie Devlin, Abraham Reichenberg, Sven Sandin
BACKGROUND: Autism spectrum disorder (ASD) has both genetic and environmental origins, including potentially maternal effects. Maternal effects describe association of one or more maternal phenotypes with liability to ASD in progeny that are independent of maternally transmitted risk alleles. While maternal effects could play an important role, consistent with association to maternal traits such as immune status, no study has estimated maternal, additive genetic, and environmental effects in ASD...
September 21, 2017: Biological Psychiatry
Joanna Sztuba-Solinska, Stuart F J Le Grice
As the notion of small molecule targeting of regulatory viral and cellular RNAs gathers momentum, understanding their structure, and variations thereof, in the appropriate biological context will play a critical role. This is especially true of the ∼1100-nt polyadenylated nuclear (PAN) long non-coding (lnc) RNA of Kaposi's sarcoma herpesvirus (KSHV), whose interaction with viral and cellular proteins is central to lytic infection. Nuclear accumulation of PAN RNA is mediated via a unique triple helical structure at its 3' terminus (within the expression and nuclear retention element, or ENE) which protects it from deadenylation-dependent decay...
January 2, 2018: RNA Biology
D R Hirsch, D V Schiavone, A J Berkowitz, L A Morrison, T Masaoka, J A Wilson, E Lomonosova, H Zhao, B S Patel, S H Datla, S G Hoft, S J Majidi, R K Pal, E Gallicchio, L Tang, J E Tavis, S F J Le Grice, J A Beutler, R P Murelli
3,7-Dihydroxytropolones (3,7-dHTs) are highly oxygenated troponoids that have been identified as lead compounds for several human diseases. To date, structure-function studies on these molecules have been limited due to a scarcity of synthetic methods for their preparation. New synthetic strategies towards structurally novel 3,7-dHTs would be valuable in further studying their therapeutic potential. Here we describe the successful adaptation of a [5 + 2] oxidopyrilium cycloaddition/ring-opening for 3,7-dHT synthesis, which we apply in the synthesis of a plausible biosynthetic intermediate to the natural products puberulic and puberulonic acid...
December 19, 2017: Organic & Biomolecular Chemistry
Chloé Plet, Kliti Grice, Anais Pagès, Michael Verrall, Marco J L Coolen, Wolfgang Ruebsam, William D A Rickard, Lorenz Schwark
Carbonate concretions are known to contain well-preserved fossils and soft tissues. Recently, biomolecules (e.g. cholesterol) and molecular fossils (biomarkers) were also discovered in a 380 million-year-old concretion, revealing their importance in exceptional preservation of biosignatures. Here, we used a range of microanalytical techniques, biomarkers and compound specific isotope analyses to report the presence of red and white blood cell-like structures as well as platelet-like structures, collagen and cholesterol in an ichthyosaur bone encapsulated in a carbonate concretion from the Early Jurassic (~182...
October 23, 2017: Scientific Reports
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"