keyword
MENU ▼
Read by QxMD icon Read
search

Peripheral stem cell collection

keyword
https://www.readbyqxmd.com/read/28202859/experience-of-peripherally-inserted-central-venous-catheter-in-patients-with-hematologic-diseases
#1
Yoshinori Hashimoto, Takanori Fukuta, Junko Maruyama, Hiromi Omura, Takayuki Tanaka
Objective Although use of the peripherally inserted central venous catheter (PICC) has become increasingly common, there are few reports of PICCs used for patients with hematologic diseases. In this study, we analyzed the safety of PICC placement in patients with hematologic diseases where PICCs had been placed to perform blood collection, blood transfusion, drug administration, and hematopoietic stem cell transplantation. Methods This study included 142 PICCs placed in 95 patients managed at our department from November 2013 to December 2015...
2017: Internal Medicine
https://www.readbyqxmd.com/read/28176337/how-fish-color-their-skin-a-paradigm-for-development-and-evolution-of-adult-patterns-multipotency-plasticity-and-cell-competition-regulate-proliferation-and-spreading-of-pigment-cells-in-zebrafish-coloration
#2
Christiane Nüsslein-Volhard, Ajeet Pratap Singh
Pigment cells in zebrafish - melanophores, iridophores, and xanthophores - originate from neural crest-derived stem cells associated with the dorsal root ganglia of the peripheral nervous system. Clonal analysis indicates that these progenitors remain multipotent and plastic beyond embryogenesis well into metamorphosis, when the adult color pattern develops. Pigment cells share a lineage with neuronal cells of the peripheral nervous system; progenitors propagate along the spinal nerves. The proliferation of pigment cells is regulated by competitive interactions among cells of the same type...
February 3, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/28164682/comparison-of-simultaneous-cd34-and-cd3-quantification-with-a-modified-stem-cell-enumeration-kit-on-two-different-flow-cytometers
#3
Julian Strobel, Barbara Hauck-Dlimi, Volker Weisbach, Reinhold Eckstein, Juergen Zingsem, Erwin Strasser
BACKGROUND: Quantification of CD34+ cells in peripheral blood stem cell apheresis is normally performed by single platform flow cytometric measurements according to the ISHAGE protocol. Peripheral blood stem cell concentrates (PBSC) produced by apheresis normally contain many T cells. Those T cells can be used for production of donor lymphocyte infusion doses, if abundant amounts of CD34+ cells have been collected. Therefore, it is of interest to know both the CD3+ and the CD34+ cell count of allogeneic PBSC...
November 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28150319/factors-influencing-platelet-clumping-during-peripheral-blood-hematopoietic-stem-cell-collection
#4
Gagan Mathur, Sarah L Mott, Laura Collins, Gail A Nelson, C Michael Knudson, Annette J Schlueter
BACKGROUND: Platelet clumping is a common occurrence during peripheral blood hematopoietic stem cell (HSC) collection using the Spectra Optia mononuclear cell (MNC) protocol. If clumping persists, it may prevent continuation of the collection and interfere with proper MNC separation. This study is the first to report the incidence of clumping, identify precollection factors associated with platelet clumping, and describe the degree to which platelet clumping interferes with HSC product yield...
February 1, 2017: Transfusion
https://www.readbyqxmd.com/read/28139098/predictors-for-successful-pbsc-collection-on-the-fourth-day-of-g-csf-induced-mobilization-in-allogeneic-stem-cell-donors
#5
Anja van Oostrum, Jaap Jan Zwaginga, Sandra Croockewit, Jacqueline Overdevest, Mirjam Fechter, Bart Ruiterkamp, Anneke Brand, Tanja Netelenbos
Peripheral blood stem cells (PBSCs) used for allogeneic transplantation are collected by apheresis after pre-treatment of donors with G-CSF. Using modern apheresis devices stem cells can be collected more efficiently. It was studied whether collection on the 4th instead of the 5th day after initiation of G-CSF treatment might be feasible. Stem cell yields that could have been collected on day 4 were calculated in two cohorts treated with 10 µg/kg G-CSF once daily (n = 106, cohort I) or 5 µg/kg twice daily schedule (n = 85, cohort II)...
January 31, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28115876/management-of-psychiatric-complications-in-unrelated-donor-before-unrelated-peripheral-hematopoietic-stem-cell-collections
#6
Olivier Hequet, Valerie Mialou, Francoise Audat, Eric Wattel, Valerie Chapel, Damiela Revesz, Jean-Piere Jouet, Brigitte Fisseaux, Mohamed Saoud, Mauricette Michallet
Allogeneic hematopoietic stem cell transplantation can efficiently treat patients with severe hematological diseases. A human leukocyte antigen-compatible donor is required for performing transplantation. The occurrence of unexpected acute severe diseases in a donor can compromise the feasibility of allogeneic hematopoietic stem cell transplantation. However, when a severe health problem occurs in a donor while the recipient has already received a conditioning regimen, hematologists have to find the best solutions for the recipient, while the team in charge of the donor has to find the best medical solutions for the donor...
2017: Journal of Blood Medicine
https://www.readbyqxmd.com/read/28111240/efficiency-of-autologous-stem-cell-collection-comparison-of-three-different-cell-separators
#7
REVIEW
Véronique Deneys, Annick Fabry, Maryse Van Hooydonk, Anne Sonet, Marc André, Marc Bourgeois, Françoise Botson
Peripheral blood progenitor cells (PBPC) infusion allows rapid haematological recovery after high dose chemotherapy. Efficient PBPC collection is therefore essential as rescue therapy for transplantation. In order to validate a new equipment (ComTec(®), Fresenius Kabi), we compared the efficiency of three cell separators for PBPC collection in patients with haematological malignant diseases. From June 2014 to December 2015, 83 PBPC were collected in 48 patients. Three aphaeresis machines were used: Cobe Spectra(®) (Terumo BCT, 11), Amicus(®) (Fenwall, 30), and ComTec(®) (Fresenius Kabi, 42)...
December 30, 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28109298/neuroregenerative-potential-of-intravenous-g-csf-and-autologous-peripheral-blood-stem-cells-in-children-with-cerebral-palsy-a-randomized-double-blind-cross-over-study
#8
Wee-Jin Rah, Young-Ho Lee, Jin-Hwa Moon, Hyun-Ju Jun, Hye-Ryeong Kang, Hani Koh, Hye Jung Eom, Ji Young Lee, Young Jun Lee, Ji Young Kim, Yun-Young Choi, Kyeongil Park, Mi Jung Kim, Seung-Hyun Kim
OBJECTIVE: We performed a randomized, double-blind, cross-over study to assess the neuroregenerative potential of intravenous granulocyte colony-stimulating factor (G-CSF) followed by infusion of mobilized peripheral blood mononuclear cells (mPBMCs) in children with cerebral palsy (CP). METHODS: Children with non-severe CP were enrolled in this study. G-CSF was administered for 5 days, then mPBMCs were collected by apheresis and cryopreserved. One month later (M1), recipients were randomized to receive either mPBMCs or a placebo infusion, and these treatment groups were switched at 7 months (M7) and observed for another 6 months (M13)...
January 21, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28101890/g-csf-mobilized-peripheral-blood-stem-cell-collection-for-allogeneic-transplantation-in-healthy-donors-analysis-of-factors-affecting-yield
#9
Maciej Machaczka, Hans Hägglund, Emma Staver, Monika Joks, Moustapha Hassan, Björn Engelbrekt Wahlin, Ulla Axdorph Nygell
Mobilized PBSC are the main source for allogeneic HSCT. We aimed to evaluate factors that affect CD34+ cell yield including the donor's age, gender, BSA, processed blood volume and the method of G-CSF dose calculation. Data from 170 healthy donors were analyzed. The concentration of CD34+ cells in the peripheral blood (PB) and the processed volume of blood were significantly correlated to CD34+ cells yield (P < .00005 and P < .001, respectively). The G-CSF dose per m(2) was significantly correlated to the concentration of CD34+ cells in the PB (P = ...
January 19, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28088963/-clinical-characteristics-and-prognosis-in-12-patients-with-adult-t-cell-leukemia-lymphoma-confirmed-by-htlv-1-provirus-gene-detection
#10
Z J Wu, X Y Zheng, X Z Yang, T B Liu, T Yang, Z H Zheng, F Gao, C X Chen, J G Li, C Q Zhang, W Q Lin, H Y Zheng, S X Lin, J D Hu
Objective: To analyze the clinical characteristics and prognosis of adult T cell leukemia/lymphoma (ATLL). Methods: Peripheral blood samples from patients who were suspected as ATLL from March, 2013 to July, 2015, were collected for HTLV-1 provirus genes detection in genomic DNA extraction by PCR. Cases showing positive results were confirmed as ATLL. Clinical and laboratory characteristics, therapeutic outcomes and survival evaluation were collected. Results: 12 out of 23 suspected patients were confirmedly diagnosed as ATLL through HTLV-1 provirus genes detection by PCR...
December 14, 2016: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28067165/gene-therapy-in-fanconi-anemia-a-matter-of-time-safety-and-gene-transfer-tool-efficiency
#11
Els Verhoeyen, Francisco José Román Rodríguez, François-Loïc Cosset, Camille Lévy, Paula Rio
Fanconi anemia (FA) is a rare genetic syndrome characterized by progressive marrow failure. Gene therapy by infusion of FA-corrected autologous hematopoietic stem cells (HSCs) may offer a potential cure since it is a monogenetic disease with mutations in the FANC genes, coding for DNA repair enzymes (See review[1]). However, the collection of hCD34 +-cells in FA patients implies particular challenges because of the reduced numbers of progenitor cells present in their bone marrow (BM)[2] or mobilized peripheral blood[3-5]...
9, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28057639/clinical-and-immunologic-impact-of-ccr5-blockade-in-graft-versus-host-disease-prophylaxis
#12
Ryan H Moy, Austin P Huffman, Lee P Richman, Lisa Crisalli, Ximi K Wang, James A Hoxie, Rosemarie Mick, Stephen G Emerson, Yi Zhang, Robert H Vonderheide, David L Porter, Ran Reshef
Graft-versus-host disease (GVHD) is a major cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Lymphocyte trafficking via chemokine receptors such as CCR5 plays a critical role in alloreactive responses, and previous data suggest that CCR5 blockade with maraviroc results in a low incidence of visceral GVHD. However, the full scope of clinical and immunologic effects of CCR5 blockade in HSCT has not been described. We compared a cohort of patients enrolled on a trial of reduced-intensity allo-HSCT with standard GVHD prophylaxis plus maraviroc to a contemporary control cohort receiving standard GVHD prophylaxis alone...
January 5, 2017: Blood
https://www.readbyqxmd.com/read/28049042/aryl-hydrocarbon-receptor-activation-by-2-3-7-8-tetrachlorodibenzo-p-dioxin-impairs-human-b-lymphopoiesis
#13
Jinpeng Li, Ashwini S Phadnis-Moghe, Robert B Crawford, Norbert E Kaminski
The homeostasis of peripheral B cell compartment requires lifelong B lymphopoiesis from hematopoietic stem cells (HSC). As a result, the B cell repertoire is susceptible to disruptions of hematopoiesis. Increasing evidence, primarily from rodent models, shows that the aryl hydrocarbon receptor (AHR) regulates hematopoiesis. To study the effects of persistent AHR activation on human B cell development, a potent AHR agonist and known environmental contaminant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was utilized...
March 1, 2017: Toxicology
https://www.readbyqxmd.com/read/27980502/granulocyte-colony-stimulating-factor-alters-the-systemic-metabolomic-profile-in-healthy-donors
#14
Kimberley Joanne Hatfield, Guro Kristin Melve, Øystein Bruserud
INTRODUCTION: Peripheral blood stem cells mobilized by granulocyte colony-stimulating factor (G-CSF) from healthy donors are commonly used for allogeneic stem cell transplantation. The effect of G-CSF administration on global serum metabolite profiles has not been investigated before. OBJECTIVES: This study aims to examine the systemic metabolomic profiles prior to and following administration of G-CSF in healthy adults. METHODS: Blood samples were collected from 15 healthy stem cell donors prior to and after administration of G-CSF 10 µg/kg/day for 4 days...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/27965986/donor-cell-composition-and-reactivity-predict-risk-of-acute-graft-versus-host-disease-after-allogeneic-hematopoietic-stem-cell-transplantation
#15
Darius Sairafi, Arwen Stikvoort, Jens Gertow, Jonas Mattsson, Michael Uhlin
Background. Graft-versus-host disease (GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation (HSCT). We designed a functional assay for assessment of individual risk for acute GVHD. Study Design and Methods. Blood samples were collected from patients and donors before HSCT. Two groups of seven patients each were selected, one in which individuals developed acute GVHD grades II-IV and one in which none showed any clinical signs of GVHD. Peripheral blood mononuclear cells (PBMCs) isolated from donors were incubated in mixed lymphocyte cultures (MLCs) with recipient PBMCs...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/27940274/direct-conversion-of-human-fibroblasts-into-neural-progenitors-using-transcription-factors-enriched-in-human-esc-derived-neural-progenitors
#16
Pei-Shan Hou, Ching-Yu Chuang, Chan-Hsien Yeh, Wei Chiang, Hsiao-Jung Liu, Teng-Nan Lin, Hung-Chih Kuo
Early human embryonic stem cell (hESC)-derived neural populations consist of various embryonic neural progenitors (ENPs) with broad neural developmental propensity. Here, we sought to directly convert human somatic cells into ENP-like phenotypes using hESC-ENP-enriched neural transcription factors (TFs). We demonstrated that induced ENP could be efficiently converted from human fibroblasts using two TF combinations. The iENPs exhibit cellular and molecular characteristics resembling hESC-ENPs and can give rise to astrocytes, oligodendrocytes, and functional neuronal subtypes of the central and peripheral nervous system...
January 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/27939210/studies-of-innate-immune-systems-against-human-cells
#17
Rieko Sakai, Etsuko Kitano, Akira Maeda, Pei-Chi Lo, Hiroshi Eguchi, Masahito Watanabe, Hiroshi Nagashima, Hiroomi Okuyama, Shuji Miyagawa
BACKGROUND: Pigs are frequently used as animal models for experiments in the surgical field, including allo- and xeno-transplantation. Regeneration studies, including studies dealing with human- and monkey-induced pluripotent stem cells (iPSC), have gradually progressed, with pigs sometimes being used as the scaffold. However, the immunological response of pigs against humans, especially innate immunities, remain unclear. This study reports on a comprehensive study of pig innate immunity against humans...
December 7, 2016: Transplant Immunology
https://www.readbyqxmd.com/read/27938475/imbalanced-production-of-reactive-oxygen-species-and-mitochondrial-antioxidant-sod2-in-fabry-disease-specific-human-induced-pluripotent-stem-cell-differentiated-vascular-endothelial-cells
#18
Wei-Lien Tseng, Shih-Jie Chou, Huai-Chih Chiang, Mong-Lien Wang, Chian-Shiu Chien, Kuan-Hsuan Chen, Hsin-Bang Leu, Chien-Ying Wang, Yuh-Lih Chang, Yung-Yang Liu, Yuh-Jyh Jong, Shing-Jong Lin, Shih-Hwa Chiou, Wen-Chung Yu, Shinn-Zong Lin
Fabry disease (FD) is an X-linked inherited lysosomal storage disease caused by α-galactosidase A (GLA) deficiency. Progressive intracellular accumulation of globotriaosylceramide (Gb3) is considered to be pathogenically responsible for the phenotype variability of FD that causes cardiovascular dysfunction; however, molecular mechanisms underlying the impairment of FD-associated cardiovascular tissues remain unclear. In this study, we reprogrammed human induced pluripotent stem cells (hiPSC) from peripheral blood cells of patients with FD (FD-iPSCs); subsequently differentiated them into vascular endothelial-like cells (FD-ECs) expressing CD31, VE-cadherin, and vWF; and investigated their ability to form vascular tube-like structures...
6, 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27934603/generation-and-characterization-of-an-induced-pluripotent-stem-cell-ipsc-line-from-a-patient-with-clozapine-resistant-schizophrenia
#19
Fabio Marsoner, Matteo Marcatili, Thodoris Karnavas, Daniele Bottai, Armando D'Agostino, Silvio Scarone, Luciano Conti
Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as "super-refractory") Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments...
November 10, 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27934596/establishment-of-an-induced-pluripotent-stem-cell-ipsc-line-from-a-patient-with-clozapine-responder-schizophrenia
#20
Matteo Marcatili, Fabio Marsoner, Armando D'Agostino, Thodoris Karnavas, Daniele Bottai, Silvio Scarone, Luciano Conti
Peripheral blood mononuclear cells (PBMCs) were collected from a patient with treatment-refractory Schizophrenia who presented an exceptional clinical response to Clozapine. iPSC lines were established with a non-integrating reprogramming system based on Sendai virus. A footprint-free hiPSC line was characterized to confirm the expression of the main endogenous pluripotency markers and have a regular karyotype. Pluripotency was confirmed by differentiation into cells belonging to the three germ layers. This hiPSC line represents a valuable tool to study the molecular, biochemical and electrophysiological properties of mature neuronal populations belonging to Clozapine responder patients with a severe form of Schizophrenia...
November 9, 2016: Stem Cell Research
keyword
keyword
52735
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"