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Peripheral stem cell collection

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https://www.readbyqxmd.com/read/29773496/reflections-on-methodical-approaches-to-hematopoietic-stem-cell-collection-in-children
#1
REVIEW
Claudia Del Fante, Jerard Seghatchian, Cesare Perotti
Pediatric peripheral blood stem cell collection (PBSC) is challenging because it has potentially more side effects than in adults due to the small body mass and unique physiology of children. The extracorporeal volume of the cell separator device, poor venous access and metabolic complications due to citrate toxicity are the main problems to face during PBSC collection. These aspects are more relevant in very low body weight (BW) children of 20 kg or lower. An efficient, experienced and well-prepared team of pediatricians, apheresis physicians and nurses, and physicians involved in CVC positioning is crucial to performing a safe PBSC collection...
May 9, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29764770/haploidentical-stem-cell-transplantation-bone-marrow-vs-peripheral-blood
#2
REVIEW
Zafer Gulbas
Hematopoietic stem cells may be obtained by collection of bone marrow, mobilization and collection of peripheral blood stem cells or umbilical cord blood. Transplantation of peripheral blood hematopoietic cells has increased due to faster engraftment and practicability in both the related, unrelated or haploidentical setting. We reviewed the question of which stem cell source - bone marrow (BM) or peripheral blood (PBSC) - is the most suitable for individuals undergoing haploidentical stem cell transplantation...
April 19, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29760736/comparison-of-immunological-characteristics-of-mesenchymal-stem-cells-from-the-periodontal-ligament-umbilical-cord-and-adipose-tissue
#3
Jin-Hee Kim, Chris H Jo, Hang-Rae Kim, Young-Il Hwang
Mesenchymal stem cells (MSCs) are of therapeutic importance in the fields of regenerative medicine and immunological diseases. Accordingly, studies evaluating MSCs for clinical applications are increasing. In this study, we characterized MSCs from the periodontal ligament, umbilical cord (UC-MSCs), and adipose tissue, which were relatively easy to obtain with limited ethical concerns regarding their acquisition, and compared their immunological characteristics. Among MSCs isolated from the three different tissues, UC-MSCs grew the fastest in vitro ...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29744773/cd271-cxcr7-cxcr4-and-cd133-stem-progenitor-cells-and-clinical-characteristics-of-acute-ischemic-stroke-patients
#4
Anna Gójska-Grymajło, Maciej Zieliński, Dariusz Gąsecki, Kamil Kowalczyk, Mariusz Kwarciany, Barbara Seroczyńska, Walenty M Nyka
Ischemic stroke causes mobilization of various groups of progenitor cells from bone marrow to bloodstream and this correlates with the neurological status of stroke patients. The goal of our study was to identify the activity of chosen progenitor/stem cells in the peripheral blood of acute ischemic stroke patients in the first 7 days after the incident, through associations between the levels of the cells and clinical features of the patients. Thirty-three acute ischemic stroke patients and 15 non-stroke control subjects had their venous blood collected repeatedly in order to assess the levels of the CD45-CD34 + CD271+, the CD45-CD34 + CXCR4+, the CD45-CD34 + CXCR7+, and the CD45-CD34 + CD133+ stem/progenitor cells by means of flow cytometry...
May 9, 2018: Neuromolecular Medicine
https://www.readbyqxmd.com/read/29710430/the-nasal-cavity-of-the-rat-and-mouse-source-of-mesenchymal-stem-cells-for-treatment-of-peripheral-nerve-injury
#5
Rui Damásio Alvites, Ana Rita Caseiro, Sílvia Santos Pedrosa, Mariana Esteves Vieira, Artur S P Varejão, Ana Colette Maurício
The nasal cavity performs several crucial functions in mammals, including rodents, being involved in respiration, behavior, reproduction and olfaction. Its anatomical structure is complex and divided into several regions, including the olfactory recess where the olfactory mucosa is located and where the capture and interaction with the environmental odorants occurs. Among the cells of this region are the olfactory mucosa mesenchymal stem cells, whose location raises the possibility that these cells could be involved in the peculiar ability of the olfactory nerve to regenerate continuously throughout life, although this relationship has not yet been confirmed...
April 30, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29709615/common-proteomic-profiles-of-induced-pluripotent-stem-cell-derived-three-dimensional-neurons-and-brain-tissue-from-alzheimer-patients
#6
Mei Chen, Han-Kyu Lee, Lauren Moo, Eugene Hanlon, Thor Stein, Weiming Xia
We established a unique platform for proteomic analysis of cultured three-dimensional (3D) neurons and brain tissue from Alzheimer's disease (AD) patients. We collected peripheral blood mononuclear cells (PBMC), converted PBMC to induced pluripotent stem cell (iPSC) lines, and differentiated the iPSC into human 3D neuro-spheroids. The postmortem brain tissue from the superior frontal cortex, inferior frontal cortex and cerebellum area of the AD patients was compared to the same regions from the control subjects...
April 27, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29705566/vinorelbine-cyclophosphamide-compared-to-cyclophosphamide-in-peripheral-blood-stem-cell-mobilization-for-multiple-myeloma
#7
Sanjay de Mel, Yunxin Chen, Adeline Lin, Teck Guan Soh, Melissa Ooi, Eng Soo Yap, Lara Kristina Sioco Donato, Nurul Aidah Abdul Halim, Joanna Mah, Karen Lim, Li Mei Poon, Belinda Tan, Yelly, Hui Li Lim, Liang Piu Koh, Bee Choo Tai, Zhaojin Chen, Wee Joo Chng, Satish Kumar Gopalakrishnan, Lip Kun Tan
BACKGROUND: High dose Cyclophosphamide (Cy) and Vinorelbine Cyclophosphamide (Vino-Cy) are stem cell (SC) mobilisation options for patients with multiple myeloma (MM). We present a comparison of mobilisation outcomes using these regimens. PATIENTS AND METHODS: Vino-Cy patients received Vinorelbine 25 mg/m2 on day 1, cyclophosphamide 1500 mg/m2 on day 2, and pegylated GCSF on day 4 or GCSF 10 mcg/kg/day from day 4 onwards. Cy patients were given cyclophosphamide 4000 mg/m2 on day 1 and GCSF10 mcg/kg/day from day 5 onwards...
April 23, 2018: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/29692815/n-cadherin-upregulation-promotes-the-neurogenic-differentiation-of-menstrual-blood-derived-endometrial-stem-cells
#8
Yanli Liu, Fen Yang, Shengying Liang, Qing Liu, Sulei Fu, Zhenyu Wang, Ciqing Yang, Juntang Lin
Peripheral nerve injuries are typically caused by either trauma or medical disorders, and recently, stem cell-based therapies have provided a promising treatment approach. Menstrual blood-derived endometrial stem cells (MenSCs) are considered an ideal therapeutic option for peripheral nerve repair due to a noninvasive collection procedure and their high proliferation rate and immunological tolerance. Here, we successfully isolated MenSCs and examined their biological characteristics including their morphology, multipotency, and immunophenotype...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29684429/simultaneous-in-vitro-generation-of-human-cd34-derived-dendritic-cells-and-mast-cells-from-non-mobilized-peripheral-blood-mononuclear-cells
#9
Pavla Taborska, Jirina Bartunkova, Daniel Smrz
Dendritic cells (DCs) and mast cells (MCs) are key players of the immune system, often coming in close proximity in peripheral tissues. The interplay of these cells is, however, still poorly understood especially with regards to human cells. The reason for that is the absence of a well established in vitro human cell-based study system that would allow a simultaneous preparation of both cell types. In this study, we show a method for simultaneous generation of DCs and MCs from CD34+ stem cell progenitors that were isolated from the non-adherent fraction of non-mobilized peripheral blood mononuclear cells of healthy donors...
April 20, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29662864/expression-of-adhesion-molecules-on-cd34-cells-from-steady-state-bone-marrow-before-and-after-mobilization-and-their-association-with-the-yield-of-cd34-cells
#10
Karin Zattar Cecyn, Eliza Y S Kimura, Dulce Marta S M Lima, Miyoko Yamamoto, José Orlando Bordin, José Salvador R de Oliveira
Background: Cell adhesion molecules (CAMs) expressed on hematopoietic progenitor cells (HPCs), endothelial cells, and stromal cells play a pivotal role in the mobilization of CD34+ cells. Herein, we conducted a non-randomized peripheral blood stem cell (PBSC) mobilization study aimed to compare the potential differences in the expressions of several CAMs and chemokines on CD34+ cells obtained from bone marrow aspirate before and after HPC mobilization from patients with hematologic malignancies and healthy donors...
March 2018: Blood Research
https://www.readbyqxmd.com/read/29658352/chronic-subdural-collection-overlying-an-intra-axial-hemorrhagic-lesion-in-chronic-myelomonocytic-leukemia-special-report-and-review-of-the-literature
#11
Anne-Laure Bernat, Stefano Maria Priola, Ahmad Elsawy, Faisal Farrash, Shervin Taslimi, Fred Gentili
Chronic myelomonocytic leukaemia (CMML) is a clonal hematopoietic stem cell disorder characterized by the presence of an absolute monocytosis in the peripheral blood (>1 x 109 /L) and the presence of myelodysplastic and myeloproliferative features in the bone marrow. Involvement of the central nervous system (CNS) is uncommon in CMML. Areas covered: Herein described is a case report of a CMML patient who presents with symptomatic chronic subdural collection overlying a haemorrhagic brain lesion, along with diffuse dural infiltration, after two cycles of azacytidine...
April 19, 2018: Expert Review of Neurotherapeutics
https://www.readbyqxmd.com/read/29656139/analysis-of-clinical-factors-and-outcomes-associated-with-non-utilization-of-collected-peripheral-blood-stem-cells-for-autologous-stem-cell-transplants-in-transplant-eligible-patients-with-multiple-myeloma
#12
Ankit Kansagra, Wilson I Gonsalves, Morie A Gertz, Francis K Buadi, David Dingli, Angela Dispenzieri, Martha Q Lacy, Suzanne R Hayman, Prashant Kapoor, Eli Muchtar, Taxiarchis V Kourelis, Rahma Warsame, Nelson Leung, Steven R Zeldenrust, John A Lust, S Vincent Rajkumar, Robert A Kyle, William Hogan, Shaji K Kumar
Collection and storage of peripheral blood stem cells (PBSC) for use in autologous stem cell transplantation (ASCT) upon first disease relapse is an accepted practice for eligible patients with multiple myeloma (MM). However, little is known about the factors and outcomes associated with non-utilization of these collected and stored PBSC by MM patients who intended to have a delayed ASCT. From January 1st 2004 to December 31st 2014, we identified 342 patients who underwent collection and storage of their PBSC in anticipation of a delayed ASCT upon first disease relapse...
April 12, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29596498/analyses-of-genome-wide-association-data-cytokines-and-gene-expression-in-african-americans-with-benign-ethnic-neutropenia
#13
Bashira A Charles, Matthew M Hsieh, Adebowale A Adeyemo, Daniel Shriner, Edward Ramos, Kyung Chin, Kshitij Srivastava, Neil A Zakai, Mary Cushman, Leslie A McClure, Virginia Howard, Willy A Flegel, Charles N Rotimi, Griffin P Rodgers
BACKGROUND: Benign ethnic neutropenia (BEN) is a hematologic condition associated with people of African ancestry and specific Middle Eastern ethnic groups. Prior genetic association studies in large population showed that rs2814778 in Duffy Antigen Receptor for Chemokines (DARC) gene, specifically DARC null red cell phenotype, was associated with BEN. However, the mechanism of this red cell phenotype leading to low white cell count remained elusive. METHODS: We conducted an extreme phenotype design genome-wide association study (GWAS), analyzed ~16 million single nucleotide polymorphisms (SNP) in 1,178 African-Americans individuals from the Reasons for Geographic and Racial Differences in Stroke (REGARDS) study and replicated from 819 African-American participants in the Atherosclerosis Risk in Communities (ARIC) study...
2018: PloS One
https://www.readbyqxmd.com/read/29593457/platelet-count-before-peripheral-blood-stem-cell-mobilization-is-associated-with-the-need-for-plerixafor-but-not-with-the-collection-result
#14
Marc-Andrea Baertsch, Katharina Kriegsmann, Petra Pavel, Thomas Bruckner, Michael Hundemer, Mark Kriegsmann, Anthony D Ho, Hartmut Goldschmidt, Patrick Wuchter
Background: A low platelet count before mobilization has recurrently been identified as risk factor for poor mobilization. Methods: To determine the relevance of this finding for peripheral blood stem cell (PBSC) mobilization, including pre-emptive or rescue plerixafor in the case of poor mobilization, we retrospectively analyzed all patients undergoing PBSC collection at our institution between January 2014 and December 2015 (n = 380). Results: In total, 99% of the patients (377/380) successfully collected a minimum of 2 × 106 CD34+ cells/kg body weight sufficient for a single transplant...
January 2018: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/29587847/high-sensitivity-virus-and-mycoplasma-screening-test-reveals-high-prevalence-of-parvovirus-b19-infection-in-human-synovial-tissues-and-bone-marrow
#15
Ken Watanabe, Koji Otabe, Norio Shimizu, Keiichirou Komori, Mitsuru Mizuno, Hisako Katano, Hideyuki Koga, Ichiro Sekiya
BACKGROUND: Latent microorganism infection is a safety concern for the clinical application of mesenchymal stem cells (MSCs). The aim of this study is to investigate the frequencies and sensitivities of the latent virus and mycoplasma infections in synovium, bone marrow, peripheral blood cells, and blood plasma and cultured synovial MSCs. METHODS: Total DNA and RNA of the synovium (n = 124), bone marrow (n = 123), peripheral blood cells (n = 121), plasma (n = 121), and 14-day cultured synovial MSCs (n = 63) were collected from patients who underwent total knee arthroplasty or anterior ligament reconstruction after written informed consents were obtained...
March 27, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29575692/effect-of-the-in-vivo-application-of-granulocyte-colony-stimulating-factor-on-nk-cells-in-bone-marrow-and-peripheral-blood
#16
Xing-Xing Yu, Ting-Ting Han, Ling-Ling Xu, Ying-Jun Chang, Xiao-Jun Huang, Xiang-Yu Zhao
Granulocyte colony-stimulating factor (G-CSF) has been widely used in the field of allogeneic haematopoietic stem cell transplantation (allo-HSCT) for priming donor stem cells from the bone marrow (BM) to peripheral blood (PB) to collect stem cells more conveniently. Donor-derived natural killer (NK) cells have important antitumour functions and immune regulatory roles post-allo-HSCT. The aim of this study was to evaluate the effect of G-CSF on donors' NK cells in BM and PB. The percentage of NK cells among nuclear cells and lymphocyte was significantly decreased and led to increased ratio of T and NK cells in BM and PB post-G-CSF in vivo application...
March 25, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29570217/impact-of-apheresis-automation-on-procedure-quality-and-predictability-of-cd34-cell-yield
#17
Nelly Besson, Mie Topholm Bruun, Thomas Stauffer Larsen, Christian Nielsen
Success of peripheral blood stem cell (PBSC) collections depends on patient biological parameters and stable apheresis device performance. We investigated product quality and factors influencing main apheresis procedure outcomes including CD34+ collection efficiency (CE), product volume or platelet CE. We also assessed different CD34+ cell yield prediction algorithms. Autologous PBSC collections by Spectra Optia from myeloma and lymphoma patients were analyzed. Complete blood count (CBC) from patient preprocedure and from collected products were assessed...
March 23, 2018: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29562779/mapping-of-the-human-placenta-experimental-evidence-of-amniotic-epithelial-cell-heterogeneity
#18
Lucia Centurione, Francesca Passaretta, Maria Antonietta Centurione, Silvia De Munari, Elsa Vertua, Antonietta Silini, Marco Liberati, Ornella Parolini, Roberta Di Pietro
The human placenta is an important source of stem cells that can be easily collected without ethical concerns since it is usually discarded after childbirth. In this study, we analyzed the amniotic membrane (AM) from the human placenta with the aim of mapping different regions with respect to their morpho-functional features and regenerative potential. AMs were obtained from 24 healthy women, undergoing a caesarean section, and mapped into 4 different regions according to their position in relation to the umbilical cord: the central, intermediate, peripheral, and reflected areas...
January 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29556970/concepts-utility-and-limitations-of-cord-blood-banking-what-clinicians-need-to-know
#19
Dhanya Lakshmi Narayanan, Shubha R Phadke
Stem cell transplantation and cord blood banking have received much popularity among general public and medical professionals in the recent past. But information about the scientific aspects, its utility and limitations is incomplete amongst laypersons as well as many medical practitioners. Stem cells differ from all other types of cells in the human body because of their ability to multiply in order to self perpetuate and differentiate into specialized cells. Stems cells could be totipotent, multipotent, pluripotent, oligopotent or unipotent depending on the type of cells that can arise or differentiate from them...
March 20, 2018: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/29555414/medical-and-ethical-considerations-on-hematopoietic-stem-cells-mobilization-for-healthy-donors
#20
V Moalic-Allain
Hematopoietic stem cell transplantation is a common procedure potentially beneficial to many individuals with cancer, hematological, or inherited disorders, and has highlighted the need of related or unrelated donors to perform allograft. Donation of hematopoietic stem cells, either through bone marrow harvest or peripheral blood stem cell collection, is well-established and widespread. Over the past two decades, the peripheral blood stem cell collection by aphaeresis has become the main source of hematopoietic stem cells for transplantation, due to faster engraftment and practicability and lower risk of relapse for high-risk patients...
March 16, 2018: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
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