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Peripheral stem cell collection

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https://www.readbyqxmd.com/read/28101890/g-csf-mobilized-peripheral-blood-stem-cell-collection-for-allogeneic-transplantation-in-healthy-donors-analysis-of-factors-affecting-yield
#1
Maciej Machaczka, Hans Hägglund, Emma Staver, Monika Joks, Moustapha Hassan, Björn Engelbrekt Wahlin, Ulla Axdorph Nygell
Mobilized PBSC are the main source for allogeneic HSCT. We aimed to evaluate factors that affect CD34+ cell yield including the donor's age, gender, BSA, processed blood volume and the method of G-CSF dose calculation. Data from 170 healthy donors were analyzed. The concentration of CD34+ cells in the peripheral blood (PB) and the processed volume of blood were significantly correlated to CD34+ cells yield (P < .00005 and P < .001, respectively). The G-CSF dose per m(2) was significantly correlated to the concentration of CD34+ cells in the PB (P = ...
January 19, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28088963/-clinical-characteristics-and-prognosis-in-12-patients-with-adult-t-cell-leukemia-lymphoma-confirmed-by-htlv-1-provirus-gene-detection
#2
Z J Wu, X Y Zheng, X Z Yang, T B Liu, T Yang, Z H Zheng, F Gao, C X Chen, J G Li, C Q Zhang, W Q Lin, H Y Zheng, S X Lin, J D Hu
Objective: To analyze the clinical characteristics and prognosis of adult T cell leukemia/lymphoma (ATLL). Methods: Peripheral blood samples from patients who were suspected as ATLL from March, 2013 to July, 2015, were collected for HTLV-1 provirus genes detection in genomic DNA extraction by PCR. Cases showing positive results were confirmed as ATLL. Clinical and laboratory characteristics, therapeutic outcomes and survival evaluation were collected. Results: 12 out of 23 suspected patients were confirmedly diagnosed as ATLL through HTLV-1 provirus genes detection by PCR...
December 14, 2016: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28067165/gene-therapy-in-fanconi-anemia-a-matter-of-time-safety-and-gene-transfer-tool-efficiency
#3
Verhoeyen Els, Francisco José Román Rodríguez, François-Loïc Cosset, Camille Lévy, Paula Rio
Fanconi anemia (FA) is a rare genetic syndrome characterized by progressive marrow failure. Gene therapy by infusion of FA-corrected autologous hematopoietic stem cells (HSCs) may offer a potential cure since it is a monogenetic disease with mutations in the FANC genes, coding for DNA repair enzymes (See review[1]). However, the collection of hCD34 +-cells in FA patients implies particular challenges because of the reduced numbers of progenitor cells present in their bone marrow (BM)[2] or mobilized peripheral blood[3-5]...
January 9, 2017: Current Gene Therapy
https://www.readbyqxmd.com/read/28057639/clinical-and-immunologic-impact-of-ccr5-blockade-in-graft-versus-host-disease-prophylaxis
#4
Ryan H Moy, Austin P Huffman, Lee P Richman, Lisa Crisalli, Ximi K Wang, James A Hoxie, Rosemarie Mick, Stephen G Emerson, Yi Zhang, Robert H Vonderheide, David L Porter, Ran Reshef
Graft-versus-host disease (GVHD) is a major cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Lymphocyte trafficking via chemokine receptors such as CCR5 plays a critical role in alloreactive responses, and previous data suggest that CCR5 blockade with maraviroc results in a low incidence of visceral GVHD. However, the full scope of clinical and immunologic effects of CCR5 blockade in HSCT has not been described. We compared a cohort of patients enrolled on a trial of reduced-intensity allo-HSCT with standard GVHD prophylaxis plus maraviroc to a contemporary control cohort receiving standard GVHD prophylaxis alone...
January 5, 2017: Blood
https://www.readbyqxmd.com/read/28049042/aryl-hydrocarbon-receptor-activation-by-2-3-7-8-tetrachlorodibenzo-p-dioxin-impairs-human-b-lymphopoiesis
#5
Jinpeng Li, Ashwini S Phadnis-Moghe, Robert B Crawford, Norbert E Kaminski
The homeostasis of peripheral B cell compartment requires lifelong B lymphopoiesis from hematopoietic stem cells (HSC). As a result, the B cell repertoire is susceptible to disruptions of hematopoiesis. Increasing evidence, primarily from rodent models, shows that the aryl hydrocarbon receptor (AHR) regulates hematopoiesis. To study the effects of persistent AHR activation on human B cell development, a potent AHR agonist and known environmental contaminant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was utilized...
December 31, 2016: Toxicology
https://www.readbyqxmd.com/read/27980502/granulocyte-colony-stimulating-factor-alters-the-systemic-metabolomic-profile-in-healthy-donors
#6
Kimberley Joanne Hatfield, Guro Kristin Melve, Øystein Bruserud
INTRODUCTION: Peripheral blood stem cells mobilized by granulocyte colony-stimulating factor (G-CSF) from healthy donors are commonly used for allogeneic stem cell transplantation. The effect of G-CSF administration on global serum metabolite profiles has not been investigated before. OBJECTIVES: This study aims to examine the systemic metabolomic profiles prior to and following administration of G-CSF in healthy adults. METHODS: Blood samples were collected from 15 healthy stem cell donors prior to and after administration of G-CSF 10 µg/kg/day for 4 days...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/27965986/donor-cell-composition-and-reactivity-predict-risk-of-acute-graft-versus-host-disease-after-allogeneic-hematopoietic-stem-cell-transplantation
#7
Darius Sairafi, Arwen Stikvoort, Jens Gertow, Jonas Mattsson, Michael Uhlin
Background. Graft-versus-host disease (GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation (HSCT). We designed a functional assay for assessment of individual risk for acute GVHD. Study Design and Methods. Blood samples were collected from patients and donors before HSCT. Two groups of seven patients each were selected, one in which individuals developed acute GVHD grades II-IV and one in which none showed any clinical signs of GVHD. Peripheral blood mononuclear cells (PBMCs) isolated from donors were incubated in mixed lymphocyte cultures (MLCs) with recipient PBMCs...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/27940274/direct-conversion-of-human-fibroblasts-into-neural-progenitors-using-transcription-factors-enriched-in-human-esc-derived-neural-progenitors
#8
Pei-Shan Hou, Ching-Yu Chuang, Chan-Hsien Yeh, Wei Chiang, Hsiao-Jung Liu, Teng-Nan Lin, Hung-Chih Kuo
Early human embryonic stem cell (hESC)-derived neural populations consist of various embryonic neural progenitors (ENPs) with broad neural developmental propensity. Here, we sought to directly convert human somatic cells into ENP-like phenotypes using hESC-ENP-enriched neural transcription factors (TFs). We demonstrated that induced ENP could be efficiently converted from human fibroblasts using two TF combinations. The iENPs exhibit cellular and molecular characteristics resembling hESC-ENPs and can give rise to astrocytes, oligodendrocytes, and functional neuronal subtypes of the central and peripheral nervous system...
January 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/27939210/studies-of-innate-immune-systems-against-human-cells
#9
Rieko Sakai, Etsuko Kitano, Akira Maeda, Pei-Chi Lo, Hiroshi Eguchi, Masahito Watanabe, Hiroshi Nagashima, Hiroomi Okuyama, Shuji Miyagawa
BACKGROUND: Pigs are frequently used as animal models for experiments in the surgical field, including allo- and xeno-transplantation. Regeneration studies, including studies dealing with human- and monkey-induced pluripotent stem cells (iPSC), have gradually progressed, with pigs sometimes being used as the scaffold. However, the immunological response of pigs against humans, especially innate immunities, remain unclear. This study reports on a comprehensive study of pig innate immunity against humans...
December 7, 2016: Transplant Immunology
https://www.readbyqxmd.com/read/27938475/imbalanced-production-of-reactive-oxygen-species-and-mitochondrial-antioxidant-sod2-in-fabry-disease-specific-human-induced-pluripotent-stem-cell-differentiated-vascular-endothelial-cells
#10
Wei-Lien Tseng, Shih-Jie Chou, Huai-Chih Chiang, Mong-Lien Wang, Chian-Shiu Chien, Kuan-Hsuan Chen, Hsin-Bang Leu, Chien-Ying Wang, Yuh-Lih Chang, Yung-Yang Liu, Yuh-Jyh Jong, Shing-Jong Lin, Shih-Hwa Chiou, Wen-Chung Yu, Shinn-Zong Lin
Fabry disease (FD) is an X-linked inherited lysosomal storage disease caused by α-galactosidase A (GLA) deficiency. Progressive intracellular accumulation of globotriaosylceramide (Gb3) is considered to be pathogenically responsible for the phenotype variability of FD that causes cardiovascular dysfunction; however, molecular mechanisms underlying the impairment of FD-associated cardiovascular tissues remain unclear. In this study, we reprogrammed human induced pluripotent stem cells (hiPSC) from peripheral blood cells of patients with FD (FD-iPSCs); subsequently differentiated them into vascular endothelial-like cells (FD-ECs) expressing CD31, VE-cadherin, and vWF; and investigated their ability to form vascular tube-like structures...
6, 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27934603/generation-and-characterization-of-an-induced-pluripotent-stem-cell-ipsc-line-from-a-patient-with-clozapine-resistant-schizophrenia
#11
Fabio Marsoner, Matteo Marcatili, Thodoris Karnavas, Daniele Bottai, Armando D'Agostino, Silvio Scarone, Luciano Conti
Peripheral Blood Mononuclear Cells (PBMCs) were collected from a patient with clozapine-resistant (also known as "super-refractory") Schizophrenia. iPSCs were established with a non-integrating Sendai virus-based reprogramming system. A footprint-free hiPSC line was characterized to express the main endogenous pluripotency markers and to retain a normal karyotype. Cells showed pluripotency competency by giving rise to progeny of differentiated cells belonging to the three germ layers. This hiPSC line represents a valuable tool to obtain mature, pathology-relevant neuronal populations in vitro that are suitable to investigate the molecular background of the schizophrenic disorder and the resultant patients' response to treatments...
November 10, 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27934596/establishment-of-an-induced-pluripotent-stem-cell-ipsc-line-from-a-patient-with-clozapine-responder-schizophrenia
#12
Matteo Marcatili, Fabio Marsoner, Armando D'Agostino, Thodoris Karnavas, Daniele Bottai, Silvio Scarone, Luciano Conti
Peripheral blood mononuclear cells (PBMCs) were collected from a patient with treatment-refractory Schizophrenia who presented an exceptional clinical response to Clozapine. iPSC lines were established with a non-integrating reprogramming system based on Sendai virus. A footprint-free hiPSC line was characterized to confirm the expression of the main endogenous pluripotency markers and have a regular karyotype. Pluripotency was confirmed by differentiation into cells belonging to the three germ layers. This hiPSC line represents a valuable tool to study the molecular, biochemical and electrophysiological properties of mature neuronal populations belonging to Clozapine responder patients with a severe form of Schizophrenia...
November 9, 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27934594/establishment-of-induced-pluripotent-stem-cell-ipsc-line-from-55-year-old-male-patient-with-hemorrhagic-moyamoya-disease
#13
Marina Cardano, Fabio Marsoner, Matteo Marcatili, Thodoris Karnavas, Jacopo Zasso, Luigi Andrea Lanterna, Luciano Conti
Peripheral blood mononuclear cells (PBMCs) were collected from 55-year old male patient with a confirmed diagnosis of hemorrhagic Moyamoya disease (MMD). PBMCs were reprogrammed using Sendai virus particles delivering the four Yamanaka factors. A footprint-free hiPSC line was characterized by the expression of pluripotency markers and a normal karyotype. These cells were able to give rise to Embryoid Bodies and to a progeny of differentiated cells belonging to the 3 germ layers. This hiPSC line represents a suitable tool for modelling in vitro MMD disease to investigate the cellular mechanisms underlying the occurrence of this pathology...
November 9, 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27934593/establishment-of-induced-pluripotent-stem-cell-ipsc-line-from-an-8-year-old-female-patient-with-ischemic-moyamoya-disease
#14
Marina Cardano, Fabio Marsoner, Jacopo Zasso, Matteo Marcatili, Thodoris Karnavas, Luigi Andrea Lanterna, Luciano Conti
Peripheral blood mononuclear cells (PBMCs) were collected from an 8-year old female patient affected by ischemic Moyamoya disease (MMD). Patient's PBMCs were reprogrammed using Sendai virus particles delivering the four Yamanaka factors. The footprint free hiPSC line expressed the major pluripotency markers and exhibited a normal karyotype. Cells were competent to give rise to progeny of differentiated cells belonging to the 3 germ layers. This hiPSC line represents a good tool to in vitro model MMD in order to shed light on the cellular and molecular mechanisms responsible for the occurrence of this syndrome...
November 5, 2016: Stem Cell Research
https://www.readbyqxmd.com/read/27919849/intraportal-mesenchymal-stem-cell-transplantation-prevents-acute-liver-failure-through-promoting-cell-proliferation-and-inhibiting-apoptosis
#15
Jian-Feng Sang, Xiao-Lei Shi, Bin Han, Tao Huang, Xu Huang, Hao-Zhen Ren, Yi-Tao Ding
BACKGROUND: Transplantation of mesenchymal stem cells (MSCs) has been regarded as a potential treatment for acute liver failure (ALF), but the optimal route was unknown. The present study aimed to explore the most effective MSCs transplantation route in a swine ALF model. METHODS: The swine ALF model induced by intravenous injection of D-Gal was treated by the transplantation of swine MSCs through four routes including intraportal injection (InP group), hepatic intra-arterial injection (AH group), peripheral intravenous injection (PV group) and intrahepatic injection (IH group)...
December 2016: Hepatobiliary & Pancreatic Diseases International: HBPD INT
https://www.readbyqxmd.com/read/27910184/clinical-significance-of-pre-transplant-circulating-cd3-cd4-cd161-cell-frequency-on-the-occurrence-of-neutropenic-infections-after-allogeneic-stem-cell-transplantation
#16
Tae Woo Kim, Sung-Eun Lee, Ji-Young Lim, Da-Bin Ryu, Young-Woo Jeon, Jae-Ho Yoon, Byung-Sik Cho, Ki-Seong Eom, Yoo-Jin Kim, Hee-Je Kim, Seok Lee, Seok-Goo Cho, Dong-Wook Kim, Jong Wook Lee, Woo-Sung Min, Chang-Ki Min
BACKGROUND: Few studies have been performed to identify factors that are associated with an increased risk of infections during the neutropenic period in patients undergoing allogeneic stem cell transplantation (allo-SCT). The aim of this study was to identify the host immune cells responsible for infections before engraftment. METHODS: A total of 282 patients who underwent allo-SCT were enrolled. Peripheral blood samples were collected before conditioning therapy...
December 2, 2016: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/27903222/sustained-elabela-gene-therapy-in-high-salt-induced-hypertensive-rats
#17
Claire A Schreiber, Sara J Holditch, Alex Generous, Yasuhiro Ikeda
BACKGROUND: Elabela (ELA) is a recently identified apelin receptor agonist essential for cardiac development, but its biology and therapeutic potential are unclear. In humans ELA transcripts are detected in embryonic stem cells, induced pluripotent stem cells, kidney, heart and blood vessels. ELA through the apelin receptor promotes angiogenesis in vitro, relaxes murine aortic blood vessels and attenuates high blood pressure in vivo. The apelin receptor when bound to its original ligand, apelin, exerts peripheral vasodilatory and positive inotropic effects, conferring cardioprotection in vivo...
November 21, 2016: Current Gene Therapy
https://www.readbyqxmd.com/read/27899238/goat-umbilical-cord-cells-are-permissive-to-small-ruminant-lentivirus-infection-in-vitro
#18
Gabrielle R Martins, Rebeca C Marinho, Rosivaldo Q Bezerra Junior, Antoniel de O Alves, Lilia M C Câmara, Luiz C Albuquerque-Pinto, Maria F da S Teixeira
Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture...
January 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27896624/autologous-bone-marrow-stem-cell-transplantation-for-the-treatment-of-ulcerative-colitis-complicated-with-herpes-zoster-a-case-report
#19
Hang Xiang, Xiaomei Zhang, Chao Yang, Wenhuan Xu, Xin Ge, Rong Zhang, Ya Qiu, Wanjun Sun, Fan Li, Tianyuan Xiang, Haixu Chen, Zheng Wang, Qiang Zeng
Ulcerative colitis (UC) is a chronic inflammatory bowel disease with continuous or recurrent symptoms. A 42-year-old male patient with intermittent diarrhea accompanied by bloody mucopurulent stools was admitted to our hospital. The diagnosis of UC was confirmed by a combination of laboratory examination, colonoscopy, and histological assay. The patient developed herpes zoster in the hospital, which challenged traditional treatments. Therefore, we performed an autologous bone marrow cells to modulate the immune system with his permission...
November 28, 2016: Frontiers of Medicine
https://www.readbyqxmd.com/read/27863757/is-it-feasible-to-use-granulocyte-colony-stimulating-factor-alone-to-mobilize-progenitor-cells-in-multiple-myeloma-patients-induced-with-a-cyclophosphamide-thalidomide-and-dexamethasone-regimen
#20
Edvan de Queiroz Crusoe, Fabiana Higashi, Gracia Aparecida Martinez, José Carlos Barros, Marcelo Bellesso, Marina Rossato, Ana Cinira F Marret, Carlos Sérgio Chiattone, Vania Tietsch de Moraes Hungria
BACKGROUND: Cyclophosphamide plus thalidomide as induction for multiple myeloma patients eligible for autologous stem cell transplantation may be a limiting factor for cell mobilization. The minimum acceptable mobilized peripheral blood stem cell count to prevent deleterious effects during transplantation is 2.0×10(6) CD34(+) cells/kg. Combining other treatments to granulocyte-colony stimulating factor, such as cyclophosphamide, could overcome the mobilization limitation. The objective of this study was to assess the number of CD34(+) cells mobilized using granulocyte-colony stimulating factor with and without cyclophosphamide after induction with cyclophosphamide, thalidomide and dexamethasone...
October 2016: Revista Brasileira de Hematologia e Hemoterapia
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