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Gap junction intercellular communication

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https://www.readbyqxmd.com/read/27916369/expression-of-connexin-26-and-connexin-43-is-reduced-in-hirschsprung-s-disease
#1
David Coyle, Brian Doyle, Justin M Murphy, Anne Marie O'Donnell, John Gillick, Prem Puri
BACKGROUND: Despite having an optimal pull-through operation, some children with Hirschsprung's disease (HSCR) continue to experience persistent bowel symptoms. Coordinated colonic electrical activity depends on intercellular communication between the enteric nerves, interstitial cells of Cajal (ICCs), smooth muscle cells, and fibroblast-like (platelet-derived growth factor receptor-alpha-positive) cells. Gap junctions are low-resistance channels composed of connexin (Cx) proteins which couple cells electrically and chemically...
November 2016: Journal of Surgical Research
https://www.readbyqxmd.com/read/27903977/dioscin-augments-hsv-tk-mediated-suicide-gene-therapy-for-melanoma-by-promoting-connexin-based-intercellular-communication
#2
Jianyong Xiao, Guangxian Zhang, Bin Li, Yingya Wu, Xijuan Liu, Yuhui Tan, Biaoyan Du
Suicide gene therapy is a promising strategy against melanoma. However, the low efficiency of the gene transfer technique can limit its application. Our preliminary data showed that dioscin, a glucoside saponin, could upregulate the expression of connexins Cx26 and Cx43, major components of gap junctions, in melanoma cells. We hypothesized that dioscin may increase the bystander effect of herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) through increasing the formation of gap junctions. Further analysis showed that dioscin indeed could increase the gap junctional intercellular communication in B16 melanoma cells, resulting in more efficient GCV-induced bystander killing in B16tk cells...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27876080/effect-of-testosterone-on-the-connexin37-of-sexual-mature-mouse-cumulus-oocyte-complex
#3
Yangyang Zhang, Yang Xu, Yanrong Kuai, Sheng Wang, Qing Xue, Jing Shang
BACKGROUND: Recent researches demonstrate that pre-treatment with androgen could increase retrieved oocytes number and clinical pregnancy rate in poor ovarian response (POR) patients. In view of gap junction intercellular communication (GJIC) is important for follicular growth, and androgen plays an important role in improving prognosis of POR patients, we speculate that androgen can increase the expression of connexin in follicle cells, and improve ovarian microenvironment, thus can promote ovarian response...
November 23, 2016: Journal of Ovarian Research
https://www.readbyqxmd.com/read/27874032/connexin-32-mediated-cell-cell-communication-is-essential-for-hepatic-differentiation-from-human-embryonic-stem-cells
#4
Jinhua Qin, Mingyang Chang, Shuyong Wang, Zhenbo Liu, Wei Zhu, Yi Wang, Fang Yan, Jian Li, Bowen Zhang, Guifang Dou, Jiang Liu, Xuetao Pei, Yunfang Wang
Gap junction-mediated cell-cell interactions are highly conserved and play essential roles in cell survival, proliferation, differentiation and patterning. We report that Connexin 32 (Cx32)-mediated gap junctional intercellular communication (GJIC) is necessary for human embryonic stem cell-derived hepatocytes (hESC-Heps) during step-wise hepatic lineage restriction and maturation. Vitamin K2, previously shown to promote Cx32 expression in mature hepatocytes, up-regulated Cx32 expression and GJIC activation during hepatic differentiation and maturation, resulting in significant increases of hepatic markers expression and hepatocyte functions...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27870307/abrogation-of-gap-junctional-communication-in-es-cells-results-in-a-disruption-of-primitive-endoderm-formation-in-embryoid-bodies
#5
Philipp Wörsdörfer, Felicitas Bosen, Martina Gebhardt, Nicole Russ, Katrin Zimmermann, David Komla Kessie, Thileepan Sekaran, Angela Egert, Süleyman Ergün, Hubert Schorle, Alexander Pfeifer, Frank Edenhofer, Klaus Willecke
Gap junctional intercellular communication (GJIC) has been suggested to be involved in early embryonic development but the actual functional role remained elusive. Connexin (Cx) 43 and Cx45 are co-expressed in embryonic stem (ES) cells, form gap junctions and are considered to exhibit adhesive function and/or to contribute to the establishment of defined communication compartments. Here we describe the generation of Cx43/Cx45-double deficient mouse ES cells to achieve almost complete breakdown of GJIC. Cre-loxP induced deletion of both, Cx43 and Cx45, results in a block of differentiation in embryoid bodies without affecting pluripotency marker expression and proliferation in ES cells...
November 21, 2016: Stem Cells
https://www.readbyqxmd.com/read/27860834/regulation-of-mammalian-oocyte-meiosis-by-intercellular-communication-within-the-ovarian-follicle
#6
Laurinda Jaffe, Jeremy R Egbert
Meiotic progression in mammalian preovulatory follicles is controlled by the granulosa cells around the oocyte. Cyclic GMP (cGMP) generated in the granulosa cells diffuses through gap junctions into the oocyte, maintaining meiotic prophase arrest. Luteinizing hormone then acts on receptors in outer granulosa cells to rapidly decrease cGMP. This occurs by two complementary pathways: cGMP production is decreased by dephosphorylation and inactivation of the NPR2 guanylyl cyclase, and cGMP hydrolysis is increased by activation of the PDE5 phosphodiesterase...
November 14, 2016: Annual Review of Physiology
https://www.readbyqxmd.com/read/27826898/collective-cell-migration-of-thyroid-carcinoma-cells-a-beneficial-ability-to-override-unfavourable-substrates
#7
Liudmila Lobastova, Dominik Kraus, Alexander Glassmann, Dilaware Khan, Christian Steinhäuser, Christina Wolff, Nadine Veit, Jochen Winter, Rainer Probstmeier
PURPOSE: Tumor cell invasion and metastasis are life threatening events. Invasive tumor cells tend to migrate as collective sheets. In the present in vitro study we aimed to (i) assess whether collective tumor cells gain benefits in their migratory potential compared to single cells and (ii) to identify its putative underlying molecular mechanisms. METHODS: The migratory potential of single and collective carcinoma cells was assessed using video time lapse microscopy and cell migration assays in the absence and presence of seven potential gap junction inhibitors or the Rac1 inhibitor Z62954982...
November 8, 2016: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/27821536/stochastic-left-right-neuronal-asymmetry-in-caenorhabditis-elegans
#8
REVIEW
Amel Alqadah, Yi-Wen Hsieh, Rui Xiong, Chiou-Fen Chuang
Left-right asymmetry in the nervous system is observed across species. Defects in left-right cerebral asymmetry are linked to several neurological diseases, but the molecular mechanisms underlying brain asymmetry in vertebrates are still not very well understood. The Caenorhabditis elegans left and right amphid wing 'C' (AWC) olfactory neurons communicate through intercellular calcium signalling in a transient embryonic gap junction neural network to specify two asymmetric subtypes, AWC(OFF) (default) and AWC(ON) (induced), in a stochastic manner...
December 19, 2016: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/27809287/cx43-mediates-resistance-against-mpp%C3%A2-%C2%BA-induced-apoptosis-in-sh-sy5y-neuroblastoma-cells-via-modulating-the-mitochondrial-apoptosis-pathway
#9
In-Su Kim, Palanivel Ganesan, Dong-Kug Choi
Neuronal apoptosis in the substantia nigra par compacta (SNpc) appears to play an essential role in the pathogenesis of Parkinson's disease. However, the mechanisms responsible for the death of dopaminergic neurons are not fully understood yet. To explore the apoptotic mechanisms, we used a well-known parkinsonian toxin, 1-methyl-4-phenylpyridine (MPP⁺), to induce neuronal apoptosis in the human dopaminergic SH-SY5Y cell line. The most common method of interaction between cells is gap junctional intercellular communication (GJIC) mediated by gap junctions (GJs) formed by transmembrane proteins called connexins (Cx)...
November 1, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27807451/lifespan-extension-and-sustained-expression-of-stem-cell-phenotype-of-human-breast-epithelial-stem-cells-in-a-medium-with-antioxidants
#10
Kai-Hung Wang, An-Pei Kao, Chia-Cheng Chang, Ta-Chin Lin, Tsung-Cheng Kuo
We have previously reported the isolation and culture of a human breast epithelial cell type with stem cell characteristics (Type I HBEC) from reduction mammoplasty using the MSU-1 medium. Subsequently, we have developed several different normal human adult stem cell types from different tissues using the K-NAC medium. In this study, we determined whether this low calcium K-NAC medium with antioxidants (N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate) is a better medium to grow human breast epithelial cells...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27782134/gap-junctions-and-cancer-communicating-for-50-years
#11
Trond Aasen, Marc Mesnil, Christian C Naus, Paul D Lampe, Dale W Laird
Fifty years ago, tumour cells were found to lack electrical coupling, leading to the hypothesis that loss of direct intercellular communication is commonly associated with cancer onset and progression. Subsequent studies linked this phenomenon to gap junctions composed of connexin proteins. Although many studies support the notion that connexins are tumour suppressors, recent evidence suggests that, in some tumour types, they may facilitate specific stages of tumour progression through both junctional and non-junctional signalling pathways...
October 21, 2016: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/27780540/endometrial-androgen-signaling-and-decidualization-regulate-trophoblast-expansion-and-invasion-in-co-culture-a-time-lapse-study
#12
Thanyarat Wongwananuruk, Tomomi Sato, Takeshi Kajihara, Sachiko Matsumoto, Masumi Akita, Kazuhiro Tamura, Jan J Brosens, Osamu Ishihara
INTRODUCTION: To elucidate whether trophoblast expansion and invasion are modulated by androgen signaling in an in vitro co-culture model system with decidualizing endometrial stromal cells (ESCs). METHODS: We employed an in vitro co-culture model of early embryo implantation, consisting of human ESCs (EtsT499 cells) and spheroids generated by extravillous trophoblast (EVT) derived HTR8/Svneo. The ESCs were decidualized with 8-bromo-cAMP (8-br-cAMP) in the presence or absence of dihydrotestosterone (DHT) at various concentrations for 5 days before co-culture with EVT spheroids...
November 2016: Placenta
https://www.readbyqxmd.com/read/27748887/gefitinib-enhances-oxaliplatin-induced-apoptosis-mediated-by-src-and-pkc-modulated-gap-junction-function
#13
Jian-Feng Wu, Jie Ji, Shu-Ying Dong, Bei-Bei Li, Mei-Ling Yu, Dan-Dan Wu, Liang Tao, Xu-Hui Tong
Chemotherapeutic drug-induced apoptosis is enhanced by gap junction intercellular communication (GJIC) in a variety of tumor cells. Oxaliplatin and gefitinib are the most widely used chemotherapeutic drugs. However, the synergistic influence remains unknown in testicular cancer chemotherapy. The aim of the present study was to investigate the apoptosis induced by oxaliplatin combined with gefitinib and the potential mechanisms in I-10 testicular cancer cells. The results showed that gefitinib significantly enhanced oxaliplatin-induced apoptosis...
October 11, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27739121/protective-effects-of-connexins-in-atheromatous-plaques-in-patients-of-carotid-artery-stenosis
#14
Taizen Nakase, Tatsuya Ishikawa, Hajime Miyata
Fragility of atheromatous plaque in the internal carotid artery can be a risk of brain infarction. The activation of macrophages by oxidative stress and the vulnerability of vascular endothelial cells have been reported to participate in the fragility of atheromatous plaque. Therefore, from the view point of prevention of brain infarction, we investigated the pathological factors which may influence the stabilization of atheromatous plaque. Patients undertaking carotid endoarterectomy (CEA) were continuously screened...
October 13, 2016: Neuropathology: Official Journal of the Japanese Society of Neuropathology
https://www.readbyqxmd.com/read/27684198/a-mimic-of-the-tumor-microenvironment-a-simple-method-for-generating-enriched-cell-populations-and-investigating-intercellular-communication
#15
Jason D Domogauer, Sonia M de Toledo, Edouard I Azzam
Understanding the early heterotypic interactions between cancer cells and the surrounding non-cancerous stroma is important in elucidating the events leading to stromal activation and establishment of the tumor microenvironment (TME). Several in vitro and in vivo models of the TME have been developed; however, in general these models do not readily permit isolation of individual cell populations, under non-perturbing conditions, for further study. To circumvent this difficulty, we have employed an in vitro TME model using a cell growth substrate consisting of a permeable microporous membrane insert that permits simple generation of highly enriched cell populations grown intimately, yet separately, on either side of the insert's membrane for extended co-culture times...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27682878/the-c-terminal-domain-of-connexin43-modulates-cartilage-structure-via-chondrocyte-phenotypic-changes
#16
Raquel Gago-Fuentes, John F Bechberger, Marta Varela-Eirin, Adrian Varela-Vazquez, Benigno Acea, Eduardo Fonseca, Christian C Naus, Maria D Mayan
Chondrocytes in cartilage and bone cells population express connexin43 (Cx43) and gap junction intercellular communication (GJIC) is essential to synchronize cells for coordinated electrical, mechanical, metabolic and chemical communication in both tissues. Reduced Cx43 connectivity decreases chondrocyte differentiation and defective Cx43 causes skeletal defects. The carboxy terminal domain (CTD) of Cx43 is located in the cytoplasmic side and is key for protein functions. Here we demonstrated that chondrocytes from the CTD-deficient mice, K258stop/Cx43KO and K258stop/K258stop, have reduced GJIC, increased rates of proliferation and reduced expression of collagen type II and proteoglycans...
September 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27642298/connexin-s-connection-in-breast-cancer-growth-and-progression
#17
REVIEW
Debarshi Banerjee
Gap junctions are cell-to-cell junctions that are located in the basolateral surface of two adjoining cells. A gap junction channel is composed of a family of proteins called connexins. Gap junction channels maintain intercellular communication between two cells through the exchange of ions, small metabolites, and electrical signals. Gap junction channels or connexins are widespread in terms of their expression and function in maintaining the development, differentiation, and homeostasis of vertebrate tissues...
2016: International Journal of Cell Biology
https://www.readbyqxmd.com/read/27638711/carotenoids-biochemistry-pharmacology-and-treatment
#18
Alireza Milani, Marzieh Basirnejad, Sepideh Shahbazi, Azam Bolhassani
Carotenoids and retinoids have several similarities in biological activities such as antioxidant properties, the inhibition of malignant tumor growth, and the induction of apoptosis. Supplementation with carotenoids can influence cell growth regulation, and modulate gene expression and immune response. Epidemiologic studies have shown the correlation of high carotenoid intake from food sources with reduced risk of breast, cervical, ovarian, colorectal cancers, and cardiovascular or eye diseases. Cancer chemoprevention by dietary carotenoids involves some approaches including gap junctional intercellular communication (GJIC), growth factor signaling, cell cycle progression, differentiation-related proteins, retinoid-like receptors, antioxidant response element, nuclear receptors, AP-1 transcriptional complex, Wnt/β-Catenin pathway, and inflammatory cytokines...
September 16, 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/27628689/a-cell-based-high-throughput-assay-for-gap-junction-communication-suitable-for-assessing-connexin-43-ezrin-interaction-disruptors-using-incucyte-zoom
#19
Aleksandra R Dukic, David W McClymont, Kjetil Taskén
Connexin 43 (Cx43), the predominant gap junction (GJ) protein, directly interacts with the A-kinase-anchoring protein (AKAP) Ezrin in human cytotrophoblasts and a rat liver epithelial cells (IAR20). The Cx43-Ezrin-protein kinase (PKA) complex facilitates Cx43 phosphorylation by PKA, which triggers GJ opening in cytotrophoblasts and IAR20 cells and may be a general mechanism regulating GJ intercellular communication (GJIC). Considering the importance of Cx43 GJs in health and disease, they are considered potential pharmaceutical targets...
September 14, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27625181/mitotic-cells-form-actin-based-bridges-with-adjacent-cells-to-provide-intercellular-communication-during-rounding
#20
Tone A Fykerud, Lars M Knudsen, Max Z Totland, Vigdis Sørensen, Shiva Dahal-Koirala, Ragnhild A Lothe, Andreas Brech, Edward Leithe
In order to achieve accurate chromosome segregation, eukaryotic cells undergo a dramatic change in morphology to obtain a spherical shape during mitosis. Interphase cells communicate directly with each other by exchanging ions and small molecules via gap junctions, which have important roles in controlling cell growth and differentiation. As cells round up during mitosis, the gap junctional communication between mitotic cells and adjacent interphase cells ceases. Whether mitotic cells use alternative mechanisms for mediating direct cell-cell communication during rounding is currently unknown...
November 2016: Cell Cycle
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