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Gap junction intercellular communication

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https://www.readbyqxmd.com/read/28342022/identifying-connexin-expression-and-determining-gap-junction-intercellular-communication-in-rainbow-trout-cells
#1
Joshua Hooper, Sarah J Poynter, Stephanie J DeWitte-Orr
Gap junctions are groups of membrane-bound channels that allow the passage of small molecules and ions between cells, permitting cell-cell communication. Because of their importance in cell homeostasis, gap junction presence and function were characterized in three commonly studied rainbow trout cell lines, namely RTgill-W1, RTgutGC, and RTG-2. Firstly, gap junction presence was determined by screening for gap junction protein alpha 7 and alpha 1 (GJA7 and GJA1) presence at the transcript level and GJA7 at the protein level...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28329830/secondhand-smoke-prevalent-polycyclic-aromatic-hydrocarbon-binary-mixture-induced-specific-mitogenic-and-pro-inflammatory-cell-signaling-events-in-lung-epithelial-cells
#2
Ross S Osgood, Brad L Upham, Pierre R Bushel, Kalpana Velmurugan, Ka-Na Xiong, Alison K Bauer
Low molecular weight polycyclic aromatic hydrocarbons (LMW PAHs; < 206.3 g/mol) are prevalent and ubiquitous environmental contaminants, presenting a human health concern, and have not been as thoroughly studied as the high MW PAHs. LMW PAHs exert their pulmonary effects, in part, through P38-dependent and -independent mechanisms involving cell-cell communication and the production of pro-inflammatory mediators known to contribute to lung disease. Specifically, we determined the effects of two representative LMW PAHs, 1-methylanthracene (1-MeA) and fluoranthene (Flthn), individually and as a binary PAH mixture on the dysregulation of gap junctional intercellular communication (GJIC) and connexin 43 (Cx43), activation of mitogen activated protein kinases (MAPK), and induction of inflammatory mediators in a mouse non-tumorigenic alveolar type II cell line (C10)...
January 30, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28295002/-ultrastructural-characteristics-of-gap-junctions-in-human-glial-brain-tumors
#3
E Yu Kirichenko, A F Savchenko, D V Kozachenko, A E Matsionis, A K Logvinov
AIM: to conduct an electron microscopic study of intercellular communication in the samples of gemistocytic astrocytoma, oligodendroglioma, and glioblastoma. MATERIAL AND METHODS: Surgically resected tumor tissue fragments were fixed in 2.5% glutaraldehyde solution, afterfixed in 1% OsO4 solution, dehydrated, and embedded in epoxy resin. Ultrathin sections were examined using a Jem 1011 electron microscope (Jeol, Japan). RESULTS: Solitary and closely spaced gap junctions (GJs) formed by the thin processes that have the ultrastructure of an astroglial processes were identified in the astrocytoma samples...
2017: Arkhiv Patologii
https://www.readbyqxmd.com/read/28287521/single-cell-microinjection-for-cell-communication-analysis
#4
Anael Viana Pinto Alberto, André G Bonavita, Antonio A Fidalgo-Neto, Filipe Berçot, Luiz A Alves
Gap junctions are intercellular channels that allow the communication of neighboring cells. This communication depends on the contribution of a hemichannel by each neighboring cell to form the gap junction. In mammalian cells, the hemichannel is formed by six connexins, monomers with four transmembrane domains and a C and N terminal within the cytoplasm. Gap junctions permit the exchange of ions, second messengers, and small metabolites. In addition, they have important roles in many forms of cellular communication within physiological processes such as synaptic transmission, heart contraction, cell growth and differentiation...
February 26, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28259990/role-of-gap-junctions-in-the-contractile-response-to-agonists-in-the-mesenteric-resistance-artery-of-rats-with-acute-hypoxia
#5
Huan Liu, Xin-Zhi Li, Min Peng, Wei Ji, Lei Zhao, Li Li, Liang Zhang, Jun-Qiang Si, Ke-Tao Ma
Hypoxic exposure results in the vascular dysfunction and reduction of vasomotor responses and thus disrupts or reduces blood flow in the resistance arteries. Connexin (Cx)-mediated gap junctional intercellular communication (GJIC) serves a critical role in the regulation of vasomotor tone and the synchronized contraction of arteries, however whether the adverse effect of hypoxia on vasomotor responses in vascular smooth muscle layer of resistance arteries is involved in changes in the GJIC and the expression of Cx43 and Cx45 remains to be elucidated...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28250650/chronic-intake-of-green-propolis-negatively-affecting-the-rat-testis
#6
Grasiela Dias de Campos Severi-Aguiar, Suellen Josine Pinto, Cristina Capucho, Camila Andrea Oliveira, Maria Aparecida Diamante, Renata Barbieri, Fabrícia Souza Predes, Heidi Dolder
BACKGROUND: Human and animal evidence suggests that environmental toxicants may have an adverse impact on male reproductive health, reducing the population's reproductive output. Owing to the renewed attraction for natural products, some of them constitute effective alternatives to mitigate these effects. Propolis is a candidate for this use because of its intrinsic properties. In many situations, it improved the testicular damage and alleviated the toxic effects induced by environmental contaminant exposure...
January 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/28245135/connexin43-controls-the-myofibroblastic-differentiation-of-bronchial-fibroblasts-from-asthmatic-patients
#7
Milena Paw, Izabela Borek, Dawid Wnuk, Damian Ryszawy, Katarzyna Piwowarczyk, Katarzyna Kmiotek, Katarzyna A Wójcik-Pszczoła, Małgorzata Pierzchalska, Zbigniew Madeja, Marek Sanak, Przemysław Błyszczuk, Marta Michalik, Jarosław Czyż
Pathologic accumulation of myofibroblasts in asthmatic bronchi is regulated by extrinsic stimuli and by the intrinsic susceptibility of bronchial fibroblasts to transforming growth factor-β (TGF-β). The specific function of gap junctions and connexins in this process remained unknown. Here, we investigated the role of connexin (Cx)43 in TGF-β-induced myofibroblastic differentiation of fibroblasts derived from bronchoscopic biopsies of asthmatic patients and non-asthmatic donors. Asthmatic fibroblasts expressed considerably higher levels of Cx43 and were more susceptible to TGF-β1-induced myofibroblastic differentiation than their non-asthmatic counterparts...
February 28, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28220297/what-roles-do-colon-stem-cells-and-gap-junctions-play-in-the-left-and-right-location-of-origin-of-colorectal-cancers
#8
James E Trosko, Heinz-Josef Lenz
This "Commentary" examines an important clinical observation that right-sided colorectal cancers appear less treatable than the left-sided cancers. The concepts of (a) the "initiation/promotion/progression" process, (b) the stem cell hypothesis, (c) the role gap junctional intercellular communication, (d) cancer cells lacking GJIC either because of the non-expression of connexin genes or of non-functional gap junction proteins, and (e) the role of the microbiome in promoting initiated colon stem cells to divide symmetrically or asymmetrically are examined to find an explanation...
February 20, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/28193563/calcium-oxidative-stress-and-connexin-channels-a-harmonious-orchestra-directing-the-response-to-radiotherapy-treatment
#9
REVIEW
Elke Decrock, Delphine Hoorelbeke, Raghda Ramadan, Tinneke Delvaeye, Marijke De Bock, Nan Wang, Dmitri V Krysko, Sarah Baatout, Geert Bultynck, An Aerts, Mathieu Vinken, Luc Leybaert
Although radiotherapy is commonly used to treat cancer, its beneficial outcome is frequently hampered by the radiation resistance of tumor cells and adverse reactions in normal tissues. Mechanisms of cell-to-cell communication and how intercellular signals are translated into cellular responses, have become topics of intense investigation, particularly within the field of radiobiology. A substantial amount of evidence is available demonstrating that both gap junctional and paracrine communication pathways can propagate radiation-induced biological effects at the intercellular level, commonly referred to as radiation-induced bystander effects (RIBE)...
February 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28190758/intercellular-signalling-cross-talk-to-kill-to-heal-and-to-rejuvenate
#10
REVIEW
Egor Y Plotnikov, Denis N Silachev, Vasily A Popkov, Ljubava D Zorova, Irina B Pevzner, Savva D Zorov, Stanislovas S Jankauskas, Valentina A Babenko, Gennady T Sukhikh, Dmitry B Zorov
Intercellular cross-talk is a fundamental process for spreading cellular signals between neighbouring and distant cells to properly regulate their metabolism, to coordinate homeostasis, adaptation and survival as a functional tissue and organ. In this review, we take a close molecular view of the underpinning molecular mechanisms of such complex intercellular communications. There are several studied forms of cell-to-cell communications considered crucial for the maintenance of multicellular organisms. The most explored is paracrine signalling which is realised through the release of diffusible signalling factors (e...
January 23, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28188112/thrombin-induces-inositol-trisphosphate-mediated-spatially-extensive-responses-in-lung-microvessels
#11
Rachel Escue, Kathirvel Kandasamy, Kaushik Parthasarathi
Activation of plasma membrane receptors initiates compartmentalized second messenger signaling. Whether this compartmentalization facilitates the preferential intercellular diffusion of specific second messengers is unclear. Toward this, the receptor-mediated agonist, thrombin, was instilled into microvessels in a restricted region of isolated blood-perfused mouse lungs. Subsequently, the thrombin-induced increase in endothelial F-actin was determined using confocal fluorescence microscopy. Increased F-actin was evident in microvessels directly treated with thrombin and in those located in adjoining thrombin-free regions...
April 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28187910/analysis-of-gap-junctional-intercellular-communications-using-a-dielectrophoresis-based-microchip
#12
Marta Tellez-Gabriel, Céline Charrier, Bénédicte Brounais-Le Royer, Mathilde Mullard, Hannah K Brown, Franck Verrecchia, Dominique Heymann
Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours - such as bone tumours - is related to gap junction intercellular communications (GJIC). Several methodologies are available for studying GJIC, based on measuring different parameters that are useful for multiple applications, such as the study of carcinogenesis for example. These methods nevertheless have several limitations...
February 6, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28181063/connexin43-and-the-intercellular-signaling-network-regulating-skeletal-remodeling
#13
REVIEW
Megan C Moorer, Joseph P Stains
PURPOSE OF THE REVIEW: This review highlights recent developments into how intercellular communication through connexin43 facilitates bone modeling and remodeling. RECENT FINDINGS: Connexin43 is required for both skeletal development and maintenance, particularly in cortical bone, where it carries out multiple functions, including preventing osteoclastogenesis, restraining osteoprogenitor proliferation, promoting osteoblast differentiation, coordinating organized collagen matrix deposition, and maintaining osteocyte survival...
February 2017: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/28177159/connexin43-mutant-patient-derived-induced-pluripotent-stem-cells-exhibit-altered-differentiation-potential
#14
Jessica L Esseltine, Qing Shao, Courtney Brooks, Jacinda Sampson, Dean H Betts, Cheryle Séguin, Dale W Laird
We present for the first time the generation of induced pluripotent stem cells (iPSCs) from a patient with a connexin-linked disease. The importance of gap junctional intercellular communication in bone homeostasis is exemplified by the autosomal dominant developmental disorder oculodentodigital dysplasia (ODDD) which is linked to mutations in the GJA1 (Cx43) gene. ODDD is characterised by craniofacial malformations, ophthalmic deficits, enamel hypoplasia and syndactyly. In addition to harboring a Cx43 p.V216L mutation, ODDD iPSCs exhibit reduced Cx43 mRNA and protein abundance when compared to control iPSCs and display impaired channel function...
February 8, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28176351/role-of-connexin-43-in-cadmium-induced-proliferation-of-human-prostate-epithelial-cells
#15
Qingping Liu, Xiaoli Ji, Zehe Ge, Haipeng Diao, Xiuli Chang, Lihua Wang, Qing Wu
Connexins (Cxs), the subunits of gap junction channels, are involved in many physiological processes. Aberrant control of Cxs and gap junction intercellular communication may contribute to many diseases, including the promotion of cancer. Cd exposure is associated with increased risk of human prostate cancer and benign prostatic hyperplasia. The roles of Cxs in the effects of Cd on the prostate have, however, not been reported previously. In this study, the human prostate epithelial cell line RWPE-1 was exposed to Cd...
February 8, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28174541/regulation-of-connexin-based-channels-by-fatty-acids
#16
REVIEW
Carlos Puebla, Mauricio A Retamal, Rodrigo Acuña, Juan C Sáez
In this mini-review, we briefly summarize the current knowledge about the effects of fatty acids (FAs) on connexin-based channels, as well as discuss the limited information about the impact FAs may have on pannexins (Panxs). FAs regulate diverse cellular functions, some of which are explained by changes in the activity of channels constituted by connexins (Cxs) or Panxs, which are known to play critical roles in maintaining the functional integrity of diverse organs and tissues. Cxs are transmembrane proteins that oligomerize into hexamers to form hemichannels (HCs), which in turn can assemble into dodecamers to form gap junction channels (GJCs)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28144277/cx43-expressed-on-bone-marrow-stromal-cells-plays-an-essential-role-in-multiple-myeloma-cell-survival-and-drug-resistance
#17
Jinxiang Fu
INTRODUCTION: Connexin-43 (Cx43), a connexin constituent of gap junctions (GJs) is mainly expressed in bone marrow stromal cells (BMSCs) and played a important role on hematopoiesis. In this study, we explored the role of gap junctions (GJs) formed by Cx43 between BMSCs and multiple myeloma (MM) cells. MATERIAL AND METHODS: qPCR and western blot assays were employed to assay Cx43 expression in three MM cell lines (RPMI 8266, U266, and XG7), freshly isolated MM cells, and bone marrow stromal cells (BMSCs)...
February 1, 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28124625/gap-junctions-and-hemichannels-communicating-cell-death-in-neurodevelopment-and-disease
#18
REVIEW
Andrei B Belousov, Joseph D Fontes, Moises Freitas-Andrade, Christian C Naus
Gap junctions are unique membrane channels that play a significant role in intercellular communication in the developing and mature central nervous system (CNS). These channels are composed of connexin proteins that oligomerize into hexamers to form connexons or hemichannels. Many different connexins are expressed in the CNS, with some specificity with regard to the cell types in which distinct connexins are found, as well as the timepoints when they are expressed in the developing and mature CNS. Both the main neuronal Cx36 and glial Cx43 play critical roles in neurodevelopment...
January 17, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28122732/na-k-atpase-regulates-intercellular-communication-in-the-vascular-wall-via-csrc-kinase-dependent-connexin43-phosphorylation
#19
Lise Hangaard, Elena V Bouzinova, Christian Staehr, Vibeke S Dam, Sukhan Kim, Zijian Xie, Christian Aalkjaer, Vladimir V Matchkov
Communication between vascular smooth muscle cells (VSMCs) is dependent on gap junctions and is regulated by the Na,K-ATPase. The Na,K-ATPase is therefore important for synchronized VSMCs oscillatory activity, i.e. vasomotion. The signaling between the Na,K-ATPase and gap junctions is unknown. We tested here the hypothesis that that this signaling involves cSrc kinase. Intercellular communication was assessed by membrane capacitance measurements of electrically coupled VSMCs. Vasomotion in isometric myograph, input resistance and synchronized [Ca(2+)]i transients were used as readout for intercellular coupling in rat mesenteric small arteries in vitro...
January 25, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28114410/a-gap-junction-protein-inx2-modulates-calcium-flux-to-specify-border-cell-fate-during-drosophila-oogenesis
#20
Aresh Sahu, Ritabrata Ghosh, Girish Deshpande, Mohit Prasad
Intercellular communication mediated by gap junction (GJ) proteins is indispensable during embryogenesis, tissue regeneration and wound healing. Here we report functional analysis of a gap junction protein, Innexin 2 (Inx2), in cell type specification during Drosophila oogenesis. Our data reveal a novel involvement of Inx2 in the specification of Border Cells (BCs), a migratory cell type, whose identity is determined by the cell autonomous STAT activity. We show that Inx2 influences BC fate specification by modulating STAT activity via Domeless receptor endocytosis...
January 2017: PLoS Genetics
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