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Gap junction intercellular communication

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https://www.readbyqxmd.com/read/29327203/loss-of-cx43-mediated-functional-gap-junction-communication-in-meningeal-fibroblasts-following-mouse-hepatitis-virus-infection
#1
Abhishek Bose, Rahul Basu, Mahua Maulik, Jayasri Das Sarma
Mouse hepatitis virus (MHV) infection causes meningoencephalitis by disrupting the neuro-glial and glial-pial homeostasis. Recent studies suggest that MHV infection alters gap junction protein connexin 43 (Cx43)-mediated intercellular communication in brain and primary cultured astrocytes. In addition to astrocytes, meningeal fibroblasts also express high levels of Cx43. Fibroblasts in the meninges together with the basal lamina and the astrocyte endfeet forms the glial limitans superficialis as part of the blood-brain barrier (BBB)...
January 11, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29321356/the-connexin-46-mutant-v44m-impairs-gap-junction-function-causing-congenital-cataract
#2
Lijuan Chen, Dongmei Su, Sijia Li, Lina Guan, Cuige Shi, Dianjun Li, Shanshan Hu, Xu Ma
Connexin 46 (Cx46) is important for gap junction channels formation which plays crucial role in the preservation of lens homeostasis and transparency. Previously, we have identified a missense mutation (p.V44M) of Cx46 in a congenital cataract family. This study aims at dissecting the potential pathogenesis of the causative mutant of cataract. Plasmids carrying wild-type (wt) and mutant (V44M) of Cx46 were constructed and expressed in Hela cells respectively.Western blotting and fluorescence microscopy were applied to analyse the expression and subcellular localization of recombinant proteins, respectively...
December 2017: Journal of Genetics
https://www.readbyqxmd.com/read/29312613/dysregulated-connexin-43-in-her2-positive-drug-resistant-breast-cancer-cells-enhances-proliferation-and-migration
#3
Elizabeth S Yeh, Christina J Williams, Carly Bess Williams, Ingrid V Bonilla, Nancy Klauber-DeMore, Stephanie L Phillips
Connexin 43 (Cx43) is a gap junction protein whose function in the development of breast cancer and in breast cancer progression remains unclear. Evidence suggests that Cx43 (GJA1) mRNA and protein expression is altered in breast tumors. However, reports indicate both increased and decreased Cx43 levels in human breast cancer samples. Studies also suggest that loss of Cx43 regulated gap junction intercellular communication is a common feature of breast malignancies that potentially correlates with histological stage...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29239327/influence-of-vascular-endothelial-growth-factor-and-radiation-on-gap-junctional-intercellular-communication-in-glioblastoma-multiforme-cell-lines
#4
Reinhardt Krcek, Pauline Latzer, Irenäus Anton Adamietz, Helmut Bühler, Carsten Theiss
Glioblastoma multiforme (GBM) is a highly aggressive glial brain tumor with an unfavorable prognosis despite all current therapies including surgery, radiation and chemotherapy. One characteristic of this tumor is a strong synthesis of vascular endothelial growth factor (VEGF), an angiogenesis factor, followed by pronounced vascularization. VEGF became a target in the treatment of GBM, for example with bevacizumab or the tyrosine kinase inhibitor axitinib, which blocks VEGF receptors. To improve patients' prognosis, new targets in the treatment of GBM are under investigations...
November 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/29236033/mast-cells-interact-with-endothelial-cells-to-accelerate-in-vitro-angiogenesis
#5
Devandir Antonio de Souza Junior, Vivian Marino Mazucato, Ana Carolina Santana, Constance Oliver, Maria Celia Jamur
Angiogenesis is a complex process that involves interactions between endothelial cells and various other cell types as well as the tissue microenvironment. Several previous studies have demonstrated that mast cells accumulate at angiogenic sites. In spite of the evidence suggesting a relationship between mast cells and angiogenesis, the association of mast cells and endothelial cells remains poorly understood. The present study aims to investigate the relationship between mast cells and endothelial cells during in vitro angiogenesis...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235124/silver-nanoparticles-increase-connexin43-mediated-gap-junctional-intercellular-communication-in-hacat-cells-through-activation-of-reactive-oxygen-species-and-mitogen-activated-protein-kinase-signal-pathway
#6
Yu Qin, Limin Han, Di Yang, Hongying Wei, Yue Liu, Junhui Xu, Herman Autrup, Furong Deng, Xinbiao Guo
Silver nanoparticles (AgNPs) are widely used in health and consumer products that routinely contact skin. However, the biological effects and possible mechanisms of AgNPs on skin remain unclear. Gap junctional intercellular communication (GJIC) plays a critical role in multicellular organisms to maintain tissue homeostasis. The aim of this study is to examine if non-coated AgNPs affect GJIC in human keratinocytes (HaCaT cells), and to identify the possible molecular mechanisms responsible for the effects. GJIC, connexin (Cx)43 protein and mRNA expression, and the effect of siRNA-mediated knockdown of Cx43 on GJIC were assessed...
December 13, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29219059/inhibition-of-intercellular-communication-between-prostate-cancer-cells-by-a-specific-anti-steap-1-single-chain-antibody
#7
Seyed-Alireza Esmaeili, Foroogh Nejatollahi, Amirhossein Sahebkar
BACKGROUND: Six-Transmembrane epithelial antigen of the prostate-1 (STEAP-1) is present at the intercellular junctions of the secretory epithelium of prostate and is overexpressed in all steps of prostate cancer. STEAP-1 acts as a transporter protein or a putative channel between cancer cells while it has limited expression in normal human tissues. This protein has been suggested as an attractive target for prostate cancer immunotherapy. OBJECTIVE: This study aimed at the development of a specific single chain fragment variable (scFv) antibody against STEAP-1 epitope and testing the inhibitory effect of the selected scFv antibody in blocking gap junctions between tumor cells...
December 7, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29209227/the-role-of-na-k-2cl-cotransporter-1-nkcc1-slc12a2-in-dental-epithelium-during-enamel-formation-in-mice
#8
Rozita Jalali, Johannes C Lodder, Behrouz Zandieh-Doulabi, Dimitra Micha, James E Melvin, Marcelo A Catalan, Huibert D Mansvelder, Pamela DenBesten, Antonius Bronckers
Na+:K+:2Cl- cotransporters (NKCCs) belong to the SLC12A family of cation-coupled Cl- transporters. We investigated whether enamel-producing mouse ameloblasts express NKCCs. Transcripts for Nkcc1 were identified in the mouse dental epithelium by RT-qPCR and NKCC1 protein was immunolocalized in outer enamel epithelium and in the papillary layer but not the ameloblast layer. In incisors of Nkcc1-null mice late maturation ameloblasts were disorganized, shorter and the mineral density of the enamel was reduced by 10% compared to wild-type controls...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29183963/distinct-moieties-underlie-biphasic-h-gating-of-connexin43-channels-producing-a-ph-optimum-for-intercellular-communication
#9
Carolina D Garciarena, Akif Malik, Pawel Swietach, Alonso P Moreno, Richard D Vaughan-Jones
Most mammalian cells can intercommunicate via connexin-assembled, gap-junctional channels. To regulate signal transmission, connexin (Cx) channel permeability must respond dynamically to physiological and pathophysiological stimuli. One key stimulus is intracellular pH (pHi), which is modulated by a tissue's metabolic and perfusion status. Our understanding of the molecular mechanism of H+ gating of Cx43 channels-the major isoform in the heart and brain-is incomplete. To interrogate the effects of acidic and alkaline pHi on Cx43 channels, we combined voltage-clamp electrophysiology with pHi imaging and photolytic H+ uncaging, performed over a range of pHi values...
November 28, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29181063/impact-of-connexin-43-coupling-on-survival-and-migration-of-multiple-myeloma-cells
#10
Yangmin Zhang, Ziyan Wang, Liying Zhang, Dongming Zhou, Yu Sun, Panjun Wang, Songguang Ju, Ping Chen, Jun Li, Jinxiang Fu
Introduction: Gap junctions (GJs) represent the best known intercellular communication (IC) system and are membrane-spanning channels that facilitate intercellular communication by allowing small signaling molecules to pass from cell to cell. In this study, we constructed an amino terminus of human Cx43 (Cx43NT-GFP), verified the overexpression of Cx43-NT in HUVEC cells and explored the impact of gap junctions (GJs) on multiple myeloma (MM). Material and methods: The levels of phosphorylated Cx43(s368) and the change of MAPK pathway associated molecules (ERK1/2, JNK, p38, NFκB) were also investigated in our cell models...
October 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29176328/cardiomyopathy-associated-gene-1-sensitive-pkc-dependent-connexin-43-expression-and-phosphorylation-in-left-ventricular-noncompaction-cardiomyopathy
#11
Yuanyuan Xie, Shenghua Liu, Shengshou Hu, Yingjie Wei
BACKGROUND/AIMS: Cardiomyopathy-associated gene 1 (CMYA1) plays an important role in embryonic cardiac development, postnatal cardiac remodeling and myocardial injury repair. Abnormal CMYA1 expression may be involved in cardiac dysplasia and primary cardiomyopathy. Our study aims to establish the relationship between CMYA1 and Left ventricular noncompaction cardiomyopathy (LVNC) pathogenesis. METHODS: We explored the effects of CMYA1 on connexins (Cx), which contribute to gap junction intercellular communication (GJIC), and the underlying signaling pathway in human normal tissues, LVNC myocardial tissues and HL1 cells by means of western blotting, RT-qPCR, immunohistochemistry, immunofluorescence, co-immunoprecipitation and scrape loading-dye transfer...
November 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29170806/environmentally-prevalent-polycyclic-aromatic-hydrocarbons-can-elicit-co-carcinogenic-properties-in-an-in-vitro-murine-lung-epithelial-cell-model
#12
Alison K Bauer, Kalpana Velmurugan, Sabine Plöttner, Katelyn J Siegrist, Deedee Romo, Peter Welge, Thomas Brüning, Ka-Na Xiong, Heiko U Käfferlein
Low molecular weight (LMW) polycyclic aromatic hydrocarbons (PAH) are the most abundant PAHs environmentally, occupationally, and are in cigarette smoke; however, little is known about their carcinogenic potential. We hypothesized that LMW PAHs act as co-carcinogens in the presence of a known carcinogen (benzo[a]pyrene (B[a]P)) in a mouse non-tumorigenic type II cell line (C10 cells). Gap junctions are commonly suppressed and inflammation induced during tumor promotion, while DNA-adduct formation is observed during the initiation stage of cancer...
November 23, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29146153/the-stress-kinase-jnk-regulates-gap-junction-cx43-gene-expression-and-promotes-atrial-fibrillation-in-the-aged-heart
#13
Jiajie Yan, Justin K Thomson, Weiwei Zhao, Xiaomin Wu, Xianlong Gao, Dominic DeMarco, Wei Kong, Min Tong, Jun Sun, Mamdouh Bakhos, Vladimir G Fast, Qingrong Liang, Sumanth D Prabhu, Xun Ai
BACKGROUND: The stress kinase c-jun N-terminal kinase (JNK) is critical in the pathogenesis of cardiac diseases associated with an increased incidence of atrial fibrillation (AF), the most common arrhythmia in the elderly. We recently discovered that JNK activation is linked to the loss of gap junction connexin43 (Cx43) and enhanced atrial arrhythmogenicity. However, direct evidence for JNK-mediated impairment of intercellular coupling (cell-cell communication) in the intact aged atrium is lacking, as is evidence for whether and how JNK suppresses Cx43 in the aged human atrium...
November 13, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29143796/angelica-sinensis-polysaccharides-ameliorate-stress-induced-premature-senescence-of-hematopoietic-cell-via-protecting-bone-marrow-stromal-cells-from-oxidative-injuries-caused-by-5-fluorouracil
#14
Hanxianzhi Xiao, Lirong Xiong, Xiaoying Song, Pengwei Jin, Linbo Chen, Xiongbin Chen, Hui Yao, Yaping Wang, Lu Wang
Myelosuppression is the most common complication of chemotherapy. Decline of self-renewal capacity and stress-induced premature senescence (SIPS) of hematopoietic stem cells (HSCs) induced by chemotherapeutic agents may be the cause of long-term myelosuppression after chemotherapy. Whether the mechanism of SIPS of hematopoietic cells relates to chemotherapeutic injury occurred in hematopoietic microenvironment (HM) is still not well elucidated. This study explored the protective effect of Angelica sinensis polysaccharide (ASP), an acetone extract polysaccharide found as the major effective ingredients of a traditional Chinese medicinal herb named Chinese Angelica (Dong Quai), on oxidative damage of homo sapiens bone marrow/stroma cell line (HS-5) caused by 5-fluorouracil (5-FU), and the effect of ASP relieving oxidative stress in HM on SIPS of hematopoietic cells...
October 28, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29132390/a-transwell-assay-that-excludes-exosomes-for-assessment-of-tunneling-nanotube-mediated-intercellular-communication
#15
Venugopal Thayanithy, Patrick O'Hare, Phillip Wong, Xianda Zhao, Clifford J Steer, Subbaya Subramanian, Emil Lou
BACKGROUND: Tunneling nanotubes (TNTs) are naturally-occurring filamentous actin-based membranous extensions that form across a wide spectrum of mammalian cell types to facilitate long-range intercellular communication. Valid assays are needed to accurately assess the downstream effects of TNT-mediated transfer of cellular signals in vitro. We recently reported a modified transwell assay system designed to test the effects of intercellular transfer of a therapeutic oncolytic virus, and viral-activated drugs, between cells via TNTs...
November 13, 2017: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29126985/spatiotemporal-expression-pattern-of-connexin-43-during-early-chick-embryogenesis
#16
Karyn Jourdeuil, Lisa A Taneyhill
During embryogenesis, a single cell develops into new tissues and organs that are made up of a number of different cell types. The assembly of the trigeminal ganglion (cranial nerve V), an important component of the peripheral nervous system, typifies this process. The trigeminal ganglia perform key sensory functions, including sensing pain and touch in the face, and arise from cells of two different progenitor populations, the neural crest and the cranial placodes. One question that remains poorly understood is how these two populations of cells interact with each other during development to form a functional ganglion...
November 7, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29115581/tramadol-attenuates-the-sensitivity-of-glioblastoma-to-temozolomide-through-the-suppression-of-cx43%C3%A2-mediated-gap-junction-intercellular-communication
#17
Lingzhi Wang, Yuexia Peng, Jianxin Peng, Min Shao, Li Ma, Zhuoli Zhu, Guocheng Zhong, Zhengyuan Xia, Huansen Huang
Analgesics and antineoplastic drugs are often used concurrently for cancer patients. Our previous study reported that gap junctions composed of connexin32 (Cx32) was implicated in the effect of analgesics on cisplatin cytotoxicity. However, the effect of analgesic on the most widely expressed connexin (Cx), connexin43 (Cx43), and whether such effect mediates the influence on chemotherapeutic efficiency remain unknown. By manipulation of Cx43 expression or gap junction function, we found that there were gap junction-dependent and independent effect of Cx43 on temozolomide (TMZ) sensitivity in U87 glioblastoma cells...
January 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29101030/the-alpha2-adrenoreceptor-agonist-dexmedetomidine-protects-against-lipopolysaccharide-induced-apoptosis-via-inhibition-of-gap-junctions-in-lung-fibroblasts
#18
Yuan Zhang, Xiaoming Tan, Lianfang Xue
The α2-adrenoceptor inducer dexmedetomidine protects against acute lung injury (ALI), but the mechanism of this effect is largely unknown. The present study investigated the effect of dexmedetomidine on apoptosis induced by lipopolysaccharide (LPS) and the relationship between this effect and gap junction intercellular communication in human lung fibroblast cell line. Flow cytometry was used to detect apoptosis induced by LPS. Parachute dye coupling assay was used to measure gap junction function, and western blot analysis was used to determine the expression levels of connexin43 (Cx43)...
October 31, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29089870/tunneling-nanotubes-and-gap-junctions-their-role-in-long-range-intercellular-communication-during-development-health-and-disease-conditions
#19
REVIEW
Jennifer Ariazi, Andrew Benowitz, Vern De Biasi, Monique L Den Boer, Stephanie Cherqui, Haifeng Cui, Nathalie Douillet, Eliseo A Eugenin, David Favre, Spencer Goodman, Karine Gousset, Dorit Hanein, David I Israel, Shunsuke Kimura, Robert B Kirkpatrick, Nastaran Kuhn, Claire Jeong, Emil Lou, Robbie Mailliard, Stephen Maio, George Okafo, Matthias Osswald, Jennifer Pasquier, Roel Polak, Gabriele Pradel, Bob de Rooij, Peter Schaeffer, Vytenis A Skeberdis, Ian F Smith, Ahmad Tanveer, Niels Volkmann, Zhenhua Wu, Chiara Zurzolo
Cell-to-cell communication is essential for the organization, coordination, and development of cellular networks and multi-cellular systems. Intercellular communication is mediated by soluble factors (including growth factors, neurotransmitters, and cytokines/chemokines), gap junctions, exosomes and recently described tunneling nanotubes (TNTs). It is unknown whether a combination of these communication mechanisms such as TNTs and gap junctions may be important, but further research is required. TNTs are long cytoplasmic bridges that enable long-range, directed communication between connected cells...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29065929/vegf-promotes-endothelial-progenitor-cell-differentiation-and-vascular-repair-through-connexin-43
#20
Lufeng Li, Huanyun Liu, Chunxin Xu, Mengyang Deng, Mingbao Song, Xuejun Yu, Shangcheng Xu, Xiaohui Zhao
BACKGROUND: Endothelial progenitor cell (EPC) differentiation is considered crucial for vascular repair. Vascular endothelial growth factor (VEGF) induces EPC differentiation, but the underlying mechanism of this phenomenon remains unclear. Connexin 43 (Cx43) is reported to be involved in the regulation of stem cell differentiation. Therefore, we sought to determine whether Cx43 is involved in VEGF-induced EPC differentiation and vascular repair. METHODS: Rat spleen-derived EPCs were cultured and treated with various concentrations of VEGF (0, 10, or 50 ng/mL), and the relationship between EPC differentiation and Cx43 expression was evaluated...
October 24, 2017: Stem Cell Research & Therapy
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