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Gap junction intercellular communication

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https://www.readbyqxmd.com/read/29780324/intercalated-disk-extracellular-nanodomain-expansion-in-patients-with-atrial-fibrillation
#1
Tristan B Raisch, Matthew S Yanoff, Timothy R Larsen, Mohammed A Farooqui, D Ryan King, Rengasayee Veeraraghavan, Robert G Gourdie, Joseph W Baker, William S Arnold, Soufian T AlMahameed, Steven Poelzing
Aims: Atrial fibrillation (AF) is the most common sustained arrhythmia. Previous evidence in animal models suggests that the gap junction (GJ) adjacent nanodomain - perinexus - is a site capable of independent intercellular communication via ephaptic transmission. Perinexal expansion is associated with slowed conduction and increased ventricular arrhythmias in animal models, but has not been studied in human tissue. The purpose of this study was to characterize the perinexus in humans and determine if perinexal expansion associates with AF...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29751558/connexin-communication-compartments-and-wound-repair-in-epithelial-tissue
#2
REVIEW
Marc Chanson, Masakatsu Watanabe, Erin M O'Shaughnessy, Alice Zoso, Patricia E Martin
Epithelial tissues line the lumen of tracts and ducts connecting to the external environment. They are critical in forming an interface between the internal and external environment and, following assault from environmental factors and pathogens, they must rapidly repair to maintain cellular homeostasis. These tissue networks, that range from a single cell layer, such as in airway epithelium, to highly stratified and differentiated epithelial surfaces, such as the epidermis, are held together by a junctional nexus of proteins including adherens, tight and gap junctions, often forming unique and localised communication compartments activated for localised tissue repair...
May 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29750972/inhibition-of-cx43-gap-junction-uncoupling-prevents-high-glucose-induced-apoptosis-and-reduces-excess-cell-monolayer-permeability-in-retinal-vascular-endothelial-cells
#3
Dongjoon Kim, Ulrik Mouritzen, Bjarne Due Larsen, Sayon Roy
The aim of this study was to investigate whether inhibition of connexin 43 gap junction-uncoupling is sufficient to prevent retinal vascular cell loss under high glucose condition and reduce cell monolayer permeability. Rat retinal endothelial cells were grown for 3, 5, and 7 days in normal (5 mM) or high glucose (30 mM) medium; in parallel, cells grown in high glucose medium were exposed for 3, 5, and 7 days to 100 nM danegaptide, which stabilizes connexin 43-mediated cell coupling. Additionally, cells grown in normal medium were treated with a connexin 43 blocker as a negative control...
May 8, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29701678/connexins-synthesis-post-translational-modifications-and-trafficking-in-health-and-disease
#4
REVIEW
Trond Aasen, Scott Johnstone, Laia Vidal-Brime, K Sabrina Lynn, Michael Koval
Connexins are tetraspan transmembrane proteins that form gap junctions and facilitate direct intercellular communication, a critical feature for the development, function, and homeostasis of tissues and organs. In addition, a growing number of gap junction-independent functions are being ascribed to these proteins. The connexin gene family is under extensive regulation at the transcriptional and post-transcriptional level, and undergoes numerous modifications at the protein level, including phosphorylation, which ultimately affects their trafficking, stability, and function...
April 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29695954/connexin-mediated-functional-and-metabolic-coupling-between-astrocytes-and-neurons
#5
Lady C Mayorquin, Andrea V Rodriguez, Jhon-Jairo Sutachan, Sonia L Albarracín
The central nervous system (CNS) requires sophisticated regulation of neuronal activity. This modulation is partly accomplished by non-neuronal cells, characterized by the presence of transmembrane gap junctions (GJs) and hemichannels (HCs). This allows small molecule diffusion to guarantee neuronal synaptic activity and plasticity. Astrocytes are metabolically and functionally coupled to neurons by the uptake, binding and recycling of neurotransmitters. In addition, astrocytes release metabolites, such as glutamate, glutamine, D-serine, adenosine triphosphate (ATP) and lactate, regulating synaptic activity and plasticity by pre- and postsynaptic mechanisms...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29678775/involvement-of-gap-junctions-in-astrocyte-impairment-induced-by-manganese-exposure
#6
Cailing Lu, Zhijuan Meng, Yiyuan He, Deqiang Xiao, Haiqing Cai, Yilu Xu, Xianghong Liu, Xinhang Wang, Laiming Mo, Ziwei Liang, Xuejing Wei, Qingqing Ao, Boying Liang, Xiyi Li, Shen Tang, Songchao Guo
Glutamate excitotoxicity, characterized as excessive glutamate stress, is considered to be involved in cerebral ischaemia, brain trauma, and neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Glutamate homeostasis disruption was highlighted in Mn neurotoxicity caused by high levels of Mn. Astrocytes, accounting for approximately 50% of the neuronal cells in the central nervous system and maintain glutamate homeostasis, are sensitive to neurotoxicity induced by Mn exposure. Astrocytes are tightly coupled with gap junctions (GJ), which are comprised of connexins, mainly connexin43 (Cx43)...
April 17, 2018: Brain Research Bulletin
https://www.readbyqxmd.com/read/29626091/the-ubiquitin-specific-protease-usp8-deubiquitinates-and-stabilizes-cx43
#7
Jian Sun, Qianwen Hu, Hong Peng, Cheng Peng, Liheng Zhou, Jinsong Lu, Chuanxin Huang
Connexin 43 (Cx43, also known as GJA1), is the most ubiquitously expressed connexin isoform in mammalian tissues. It forms intercellular gap junction (GJ) channels, enabling adjacent cells to communicate both electrically and metabolically. Cx43 is a short-lived protein which can be quickly degraded by the ubiquitin-dependent proteasomal, endolysosomal and autophagosomal pathways. Here, we report that the ubiquitin-specific peptidase 8 (USP8) interacts with and deubiquitinates Cx43. USP8 reduces both multiple monoubiquitination and polyubiquitination of Cx43 to prevent autophagy-mediated degradation...
April 6, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29589532/targeting-gap-junctions-new-insights-in-the-treatment-of-major-depressive-disorder
#8
Denis Sarrouilhe, Marc Mesnil, Catherine Dejean
BACKGROUND: Major depressive disorder (MDD) is a multifactorial chronic and debilitating mood disease with high lifetime prevalence and associated with excess mortality. Treatments of this disease are not effective in all patients showing the need to find new therapeutic targets. OBJECTIVE: This review aims to update our knowledge about the involvement of astroglial gap junctions and hemichannels in MDD and to show how they have become potential targets for the treatment of this pathology...
March 26, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29587860/roles-of-astrocytic-connexin-43-hemichannels-and-gap-junctions-in-oxygen-glucose-deprivation-reperfusion-injury-induced-neuroinflammation-and-the-possible-regulatory-mechanisms-of-salvianolic-acid-b-and-carbenoxolone
#9
Xiang Yin, Liangshu Feng, Di Ma, Ping Yin, Xinyu Wang, Shuai Hou, Yulei Hao, Jingdian Zhang, Meiying Xin, Jiachun Feng
BACKGROUND: Glia-mediated neuroinflammation is related to brain injury exacerbation after cerebral ischemia/reperfusion (I/R) injury. Astrocytic hemichannels or gap junctions, which were mainly formed by connexin-43, have been implicated in I/R damage. However, the exact roles of astrocytic hemichannels and gap junction in neuroinflammatory responses induced by I/R injury remain unknown. METHODS: Primary cultured astrocytes were subjected to OGD/R injury, an in vitro model of I/R injury...
March 27, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29587265/transforming-growth-factor-beta-1-drives-a-switch-in-connexin-mediated-cell-to-cell-communication-in-tubular-cells-of-the-diabetic-kidney
#10
Claire Hills, Gareth William Price, Mark John Wall, Timothy John Kaufmann, Sidney Chi-Wai Tang, Wai Han Yiu, Paul Edward Squires
BACKGROUND/AIMS: Changes in cell-to-cell communication have been linked to several secondary complications of diabetes, but the mechanism by which connexins affect disease progression in the kidney is poorly understood. This study examines a role for glucose-evoked changes in the beta1 isoform of transforming growth factor (TGFβ1), on connexin expression, gap-junction mediated intercellular communication (GJIC) and hemi-channel ATP release from tubular epithelial cells of the proximal renal nephron...
March 26, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29578247/benzo-a-pyrene-decreased-gap-junctional-intercellular-communication-via-calcium-calmodulin-signaling-increases-apoptosis-in-tm4-cells
#11
Xiaoli Ji, Xin Chou, Zehe Ge, Fan Ding, Hui Gao, Qing Wu
The mechanism of male reproductive toxicity induced by benzo[a]pyrene (BaP) is poorly understood. Gap junctional intercellular communication (GJIC) is known to play a critical role in maintaining spermatogenesis. The aim of the present study was to determine the toxic effects of BaP in Sertoli cells, and to explore the possibility and potential mechanisms of BaP-induced changes in the level of GJIC, and the relationship between GJIC and BaP-induced apoptosis. We treated mouse Sertoli cell lines (TM4) with different concentrations (0...
March 26, 2018: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29571253/in-vitro-inhibited-effect-of-gap-junction-composed-of-cx43-in-the-invasion-and-metastasis-of-testicular-cancer-resistanced-to-cisplatin
#12
Dandan Wu, Beibei Li, Haofeng Liu, Min Yuan, Meiling Yu, Liang Tao, Shuying Dong, Xuhui Tong
The effect of gap junction intercellular communication composed of connexin on cancer invasion/metastasis has been thoroughly explored; however, its effect on testicular cancer resistanced to chemotherapy is still unclear. In this study, we found that the capability of invasion and migration of I-10/DDP (cisplatin (DDP)-resistance) cells were elevated. Furthermore, the expression of Cx43 and the function of gap junction (GJ) in I-10/DDP cells were decreased compared with parental I-10 cells. Pharmacological inhibition of GJs by oleamide (Olea) enhanced invasion and migration...
February 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29560438/biochemical-alterations-in-inflammatory-reactive-chondrocytes-evidence-for-intercellular-network-communication
#13
Eva Skiöldebrand, Anna Thorfve, Ulrika Björklund, Pegah Johansson, Ruth Wickelgren, Anders Lindahl, Elisabeth Hansson
Chondrocytes are effectively involved in the pathophysiological processes of inflammation in joints. They form cellular processes in the superficial layer of the articular cartilage and form gap junction coupled syncytium to facilitate cell-to-cell communication. However, very little is known about their physiological cellular identity and communication. The aim with the present work is to evaluate the physiological behavior after stimulation with the inflammatory inducers interleukin-1β and lipopolysaccharide...
January 2018: Heliyon
https://www.readbyqxmd.com/read/29551292/monitoring-gap-junctional-communication-in-astrocytes-from-acute-adult-mouse-brain-slices-using-the-gap-frap-technique
#14
Chenju Yi, Jérémy Theillon, Annette Koulakoff, Hugues Berry, Christian Giaume
Intercellular communication through gap junction channels plays a key role in cellular homeostasis and in synchronizing physiological functions, a feature that is modified in number of pathological situations. In the brain, astrocytes are the cell population that expresses the highest amount of gap junction proteins, named connexins. Several techniques have been used to assess the level of gap junctional communication in astrocytes, but so far they remain very difficult to apply in adult brain tissue. Here, using specific loading of astrocytes with sulforhodamine 101, we adapted the gap-FRAP (Fluorescence Recovery After Photobleaching) to acute hippocampal slices from 9 month-old adult mice...
March 15, 2018: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/29534516/tat-gap19-and-carbenoxolone-alleviate-liver-fibrosis-in-mice
#15
Sara Crespo Yanguas, Tereza C da Silva, Isabel V A Pereira, Joost Willebrords, Michaël Maes, Marina Sayuri Nogueira, Inar Alves de Castro, Isabelle Leclercq, Guilherme R Romualdo, Luís F Barbisan, Luc Leybaert, Bruno Cogliati, Mathieu Vinken
Although a plethora of signaling pathways are known to drive the activation of hepatic stellate cells in liver fibrosis, the involvement of connexin-based communication in this process remains elusive. Connexin43 expression is enhanced in activated hepatic stellate cells and constitutes the molecular building stone of hemichannels and gap junctions. While gap junctions support intercellular communication, and hence the maintenance of liver homeostasis, hemichannels provide a circuit for extracellular communication and are typically opened by pathological stimuli, such as oxidative stress and inflammation...
March 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29531530/gap-junctions-are-involved-in-the-rescue-of-cftr-dependent-chloride-efflux-by-amniotic-mesenchymal-stem-cells-in-coculture-with-cystic-fibrosis-cfbe41o-cells
#16
Annalucia Carbone, Roberto Zefferino, Elisa Beccia, Valeria Casavola, Stefano Castellani, Sante Di Gioia, Valentina Giannone, Manuela Seia, Antonella Angiolillo, Carla Colombo, Maria Favia, Massimo Conese
We previously found that human amniotic mesenchymal stem cells (hAMSCs) in coculture with CF immortalised airway epithelial cells (CFBE41o- line, CFBE) on Transwell® filters acquired an epithelial phenotype and led to the expression of a mature and functional CFTR protein. In order to explore the role of gap junction- (GJ-) mediated intercellular communication (GJIC) in this rescue, cocultures (hAMSC : CFBE, 1 : 5 ratio) were studied for the formation of GJIC, before and after silencing connexin 43 (Cx43), a major component of GJs...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29518658/in-vitro-profiling-of-toxic-effects-of-prominent-environmental-lower-chlorinated-pcb-congeners-linked-with-endocrine-disruption-and-tumor-promotion
#17
Kateřina Pěnčíková, Lucie Svržková, Simona Strapáčová, Jiří Neča, Iveta Bartoňková, Zdeněk Dvořák, Martina Hýžďalová, Jakub Pivnička, Lenka Pálková, Hans-Joachim Lehmler, Xueshu Li, Jan Vondráček, Miroslav Machala
The mechanisms contributing to toxic effects of airborne lower-chlorinated PCB congeners (LC-PCBs) remain poorly characterized. We evaluated in vitro toxicities of environmental LC-PCBs found in both indoor and outdoor air (PCB 4, 8, 11, 18, 28 and 31), and selected hydroxylated metabolites of PCB 8, 11 and 18, using reporter gene assays, as well as other functional cellular bioassays. We focused on processes linked with endocrine disruption, tumor promotion and/or regulation of transcription factors controlling metabolism of both endogenous compounds and xenobiotics...
June 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29508067/three-dimensional-reconstruction-of-the-intercalated-disc-including-the-intercellular-junctions-by-applying-volume-scanning-electron-microscopy
#18
Bieke Vanslembrouck, Anna Kremer, Benjamin Pavie, Frans van Roy, Saskia Lippens, Jolanda van Hengel
The intercalated disc (ID) contains different kinds of intercellular junctions: gap junctions (GJs), desmosomes and areae compositae, essential for adhesion and communication between adjacent cardiomyocytes. The junctions can be identified based on their morphology when imaged using transmission electron microscopy (TEM), however, only with very limited information in the z-dimension. The application of volume EM techniques can give insight into the three-dimensional (3-D) organization of complex biological structures...
March 5, 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29487950/exchange-of-genetic-material-a-new-paradigm-in-bone-cell-communications
#19
REVIEW
Pengbin Yin, Yi Li, Houchen Lv, Yuan Deng, Yutong Meng, Licheng Zhang, Peifu Tang
An emerging concept in intercellular communication in mammals is that communication can be mediated by exchange of genetic material, mainly in the form of RNAs. In this review, we discuss recent studies that describe the trafficking of genetic material with a focus on bone cell communication. Three major carriers are discussed: gap junctions, protein-binding complexes, and genetic material exchange mediated by extracellular vesicles. While protein-level exchange has been well documented, no review has summarized the novel paradigm of cell-to-cell communication by genetic information exchange in bone tissues or its biological relevance in terms of bone homeostasis and bone-related diseases...
June 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29482519/gap-junctions-contribute-to-anchorage-independent-clustering-of-breast-cancer-cells
#20
Fabien Gava, Lise Rigal, Odile Mondesert, Elise Pesce, Bernard Ducommun, Valérie Lobjois
BACKGROUND: Cancer cell aggregation is a key process involved in the formation of clusters of circulating tumor cells. We previously reported that cell-cell adhesion proteins, such as E-cadherin, and desmosomal proteins are involved in cell aggregation to form clusters independently of cell migration or matrix adhesion. Here, we investigated the involvement of gap junction intercellular communication (GJIC) during anchorage-independent clustering of MCF7 breast adenocarcinoma cells. METHODS: We used live cell image acquisition and analysis to monitor the kinetics of MCF7 cell clustering in the presence/absence of GJIC pharmacological inhibitors and to screen a LOPAC® bioactive compound library...
February 27, 2018: BMC Cancer
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