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https://www.readbyqxmd.com/read/27820951/elevated-cytokine-levels-in-the-aqueous-humor-of-eyes-with-bullous-keratopathy-and-low-endothelial-cell-density
#1
Takefumi Yamaguchi, Kazunari Higa, Terumasa Suzuki, Naohiko Nakayama, Yukari Yagi-Yaguchi, Murat Dogru, Yoshiyuki Satake, Jun Shimazaki
Purpose: To evaluate cytokine levels in the aqueous humor (AqH) of eyes with bullous keratopathy (BK) and low endothelial cell density (ECD). Methods: A total of 145 AqH samples (60 BK, 16 low ECD, 35 corneal diseases with normal ECD, and 34 normal controls) were collected from consecutive patients who underwent corneal transplantation or cataract surgery. None of the patients had any clinically apparent inflammation at the time of AqH collection. The AqH levels of cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IFN-α, IFN-γ, monocyte chemotactic protein [MCP]-1, TNF-α, E-selectin, P-selectin, soluble intercellular adhesion molecule [sICAM]-1, granulocyte-macrophage colony-stimulating factor [GM-CSF], macrophage inflammatory protein [MIP] -1α and MIP-1β) were compared among the groups...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27802523/restoration-of-mitochondrial-integrity-telomere-length-and-sensitivity-to-oxidation-by-in-vitro-culture-of-fuchs-endothelial-corneal-dystrophy-cells
#2
Sébastien P Gendron, Mathieu Thériault, Stéphanie Proulx, Isabelle Brunette, Patrick J Rochette
Purpose: Fuchs' endothelial corneal dystrophy (FECD), a degenerative disease of the corneal endothelium that leads to vision loss, is a leading cause of corneal transplantation. The cause of this disease is still unknown, but the implication of oxidative stress is strongly suggested. In this study, we analyzed the impact of FECD on mitochondrial DNA (mtDNA) integrity and telomere length, both of which are affected by the oxidative status of the cell. Methods: We compared the levels of total mtDNA, mtDNA common deletion (4977 bp), and relative telomere length in the corneal endothelial cells of fresh Descemet's membrane-endothelium explants and cultured cells from healthy and late stage FECD subjects...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27661858/corneal-hydration-control-in-fuchs-endothelial-corneal-dystrophy
#3
Katrin Wacker, Jay W McLaren, Katrina M Kane, Keith H Baratz, Sanjay V Patel
Purpose: To assess corneal hydration control across a range of severity of Fuchs' endothelial corneal dystrophy (FECD) by measuring the percent recovery per hour (PRPH) of central corneal thickness after swelling the cornea and to determine its association with corneal morphologic parameters. Methods: Twenty-three corneas of 23 phakic FECD patients and 8 corneas of 8 healthy control participants devoid of guttae were graded (modified Krachmer scale). Effective endothelial cell density (ECDe) was determined from the area of guttae and local cell density in confocal microscopy images...
September 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27639969/existence-of-neural-crest-derived-progenitor-cells-in-normal-and-fuchs-endothelial-dystrophy-corneal-endothelium
#4
Kishore Reddy Katikireddy, Thore Schmedt, Marianne O Price, Francis W Price, Ula V Jurkunas
Human corneal endothelial cells are derived from neural crest and because of postmitotic arrest lack competence to repair cell loss from trauma, aging, and degenerative disorders such as Fuchs endothelial corneal dystrophy (FECD). Herein, we identified a rapidly proliferating subpopulation of cells from the corneal endothelium of adult normal and FECD donors that exhibited features of neural crest-derived progenitor (NCDP) cells by showing absence of senescence with passaging, propensity to form spheres, and increased colony forming efficacy compared with the primary cells...
October 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27631348/evaluation-of-visual-quality-in-patients-with-fuchs-endothelial-corneal-dystrophy
#5
Yoshinori Oie, Shinya Watanabe, Kohji Nishida
Fuchs endothelial corneal dystrophy (FECD) is a bilateral corneal endothelial dystrophy characterized by the deposition of extracellular matrix (guttae), thickening of Descemet membrane, and progressive loss of corneal endothelial cells. Progressive endothelial cellular loss leads to corneal edema and impairs visual function. In eyes with FECD, corneal edema begins in the central cornea and expands into the periphery. FECD is the most common dystrophic reason for corneal transplantation in many countries. Some patients with FECD report visual discomfort despite the absence of corneal edema...
November 2016: Cornea
https://www.readbyqxmd.com/read/27609713/splitting-of-the-recipient-s-descemet-s-membrane-in-descemet-membrane-endothelial-keratoplasty-ultrastructure-and-clinical-relevance
#6
Julia M Weller, Ursula Schlötzer-Schrehardt, Friedrich E Kruse, Theofilos Tourtas
PURPOSE: In Descemet membrane endothelial keratoplasty (DMEK), lamellar splitting of the Descemet's membrane (DM) may occur during stripping of host's DM, leaving residual DM on the recipient's DMEK interface. The purpose of this study was to determine the incidence rate of lamellar splitting of DM during DMEK and to describe the ultrastructure of DM in these eyes. DESIGN: Retrospective consecutive case series METHODS: Setting: Institutional, single-center PATIENT POPULATION: 664 eyes with Fuchs endothelial corneal dystrophy (FECD) scheduled for primary DMEK...
September 5, 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27581649/multifunctional-ion-transport-properties-of-human-slc4a11-comparison-of-the-slc4a11-b-and-slc4a11-c-variants
#7
Liyo Kao, Rustam Azimov, Xuesi M Shao, Ricardo F Frausto, Natalia Abuladze, Debra Newman, Anthony J Aldave, Ira Kurtz
Congenital hereditary endothelial dystrophy (CHED), Harboyan syndrome (CHED with progressive sensorineural deafness), and potentially a subset of individuals with late-onset Fuchs' endothelial corneal dystrophy are caused by mutations in the SLC4A11 gene that results in corneal endothelial cell abnormalities. Originally classified as a borate transporter, the function of SLC4A11 as a transport protein remains poorly understood. Elucidating the transport function(s) of SLC4A11 is needed to better understand how its loss results in the aforementioned posterior corneal dystrophic disease processes...
November 1, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27533017/racial-ethnic-differences-in-rates-of-penetrating-or-endothelial-keratoplasty-for-fuchs-endothelial-corneal-dystrophy-among-us-medicare-beneficiaries
#8
Michael A Mahr, Keith H Baratz, David O Hodge, Jay C Erie
Importance: Fuchs endothelial corneal dystrophy (FECD) is the most common indication for corneal transplant in the United States. The association between race/ethnicity and incidence of advanced FECD, defined by a need for endothelial or penetrating keratoplasty, has not been investigated. Observations: The 2014 US Medicare Limited Data Set (5% sample of 27 163 740 fee-for-service Medicare patients) was analyzed for rate of keratoplasty performed for FECD (International Classification of Diseases, Ninth Edition code 371...
October 1, 2016: JAMA Ophthalmology
https://www.readbyqxmd.com/read/27490049/microrna-29b-overexpression-decreases-extracellular-matrix-mrna-and-protein-production-in-human-corneal-endothelial-cells
#9
Tetsuya Toyono, Tomohiko Usui, Guadalupe Villarreal, Laura Kallay, Mario Matthaei, Lucas M M Vianna, Angela Y Zhu, Masahiko Kuroda, Shiro Amano, Albert S Jun
PURPOSE: MicroRNAs are small noncoding RNAs that regulate gene expression at the posttranscriptional level. We reported that levels of microRNA (miR)-29 family are decreased in corneas of patients with Fuchs endothelial corneal dystrophy (FECD). The miR-29 family regulates the production of extracellular matrix (ECM) proteins. Accumulation of ECM proteins in Descemet membrane is an important pathologic change in FECD. In this study, we transfected miR-29b into human corneal endothelial cells and tissues and evaluated ECM protein expression levels...
November 2016: Cornea
https://www.readbyqxmd.com/read/27454658/in-vivo-imaging-of-corneal-endothelial-dystrophy-in-boston-terriers-a-spontaneous-canine-model-for-fuchs-endothelial-corneal-dystrophy
#10
Sara M Thomasy, Dennis E Cortes, Alyssa L Hoehn, Allison C Calderon, Jennifer Y Li, Christopher J Murphy
PURPOSE: Boston Terriers (BTs) have a greater prevalence of corneal endothelial dystrophy (CED), in comparison to other canine breeds. Similar to Fuchs' endothelial corneal dystrophy (FECD), this condition is characterized by endothelial cell degeneration with secondary corneal edema. This study assessed corneal morphology using in vivo confocal microscopy (IVCM) and Fourier-domain optical coherence tomography (FD-OCT) in BTs with and without CED. METHODS: The corneas of 16 BTs with CED and 15 unaffected, age-matched BTs underwent clinical evaluation and were imaged using IVCM and FD-OCT...
July 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27442316/coenzyme-q10-in-the-treatment-of-corneal-edema-in-kearns-sayre-is-there-an-application-in-fuchs-endothelial-corneal-dystrophy
#11
Jocelyn Kim, Anagha Medsinge, Bharesh Chauhan, Cara Wiest, Hannah Scanga, Rachael Monaghan, William H Moore, Ken K Nischal
PURPOSE: Corneal involvement in mitochondrial disease is seldom described. Kearns-Sayre syndrome (KSS) is a mitochondrial disorder characterized by retinitis pigmentosa, external ophthalmoplegia, and heart block. We report 2 patients with KSS with corneal lesions involving the endothelium, which improved with Coenzyme Q10 (CoQ10). Based on recent research regarding the role of dysfunctional oxidative metabolism in Fuchs Endothelial Corneal Dystrophy (FECD), we propose that mitochondrial diseases and FECD share a final pathway...
September 2016: Cornea
https://www.readbyqxmd.com/read/27400733/high-throughput-sequencing-of-16s-rrna-gene-reveals-substantial-bacterial-diversity-on-the-municipal-dumpsite
#12
Kilaza Samson Mwaikono, Solomon Maina, Aswathy Sebastian, Megan Schilling, Vivek Kapur, Paul Gwakisa
BACKGROUND: Multiple types of solid waste in developing countries is disposed of together in dumpsites where there is interaction between humans, animals and the bacteria in the waste. To study the bacteria at the dumpsite and the associated risks, previous studies have focused on culturable, leaving behind a great number of unculturable bacteria. This study focuses on a more comprehensive approach to study bacteria at the dumpsite. Since the site comprised of unsorted wastes, a qualitative survey was first performed to identify the variety of solid waste as this has influence on the microbial composition...
2016: BMC Microbiology
https://www.readbyqxmd.com/read/27273977/fuchs-endothelial-corneal-dystrophy-a-controlled-prospective-study-on-visual-recovery-after-endothelial-keratoplasty
#13
Esben Nielsen, Anders Ivarsen, Simon Kristensen, Jesper Hjortdal
PURPOSE: To investigate the determining factors of vision and subjective outcome after Descemet's stripping automated endothelial keratoplasty (DSAEK) for Fuchs' endothelial dystrophy (FECD). METHODS: In a prospective study, 41 FECD patients who received DSAEK were compared to 40 cataract patients with normal corneas who received cataract surgery (control group). Subjects were recruited between March 2013 and July 2014. Observational procedures included the following: best-corrected visual acuity (BCVA), contrast sensitivity (CS), Catquest-9SF questionnaire, Scheimpflug tomography and anterior OCT...
December 2016: Acta Ophthalmologica
https://www.readbyqxmd.com/read/27158810/effect-of-descemet-membrane-endothelial-keratoplasty-on-color-vision-in-patients-with-fuchs-dystrophy
#14
David A Price, Marianne O Price, Amanda Lopez, Francis W Price
PURPOSE: To assess changes in color discernment after Descemet membrane endothelial keratoplasty (DMEK) in patients with Fuchs endothelial corneal dystrophy (FECD). METHODS: In this prospective, single-center study, the study group was composed of 26 patients with FECD, aged 46 to 85 years, who underwent DMEK as a single surgical procedure; 24 (92%) had pseudophakic and 2 (8%) had phakic eyes. The patients completed a computerized Farnsworth-Munsell 100-hue color vision test before and 1 month after DMEK...
August 2016: Cornea
https://www.readbyqxmd.com/read/27127932/uv-a-irradiation-activates-nrf2-regulated-antioxidant-defense-and-induces-p53-caspase3-dependent-apoptosis-in-corneal-endothelial-cells
#15
Cailing Liu, Dijana Vojnovic, Irene E Kochevar, Ula V Jurkunas
PURPOSE: To examine whether Nrf2-regulated antioxidant defense and p53 are activated in human corneal endothelial cells (CEnCs) by environmental levels of ultraviolet A (UV-A), a known stimulator of oxidative stress. METHODS: Immortalized human CEnCs (HCEnCi) were exposed to UV-A fluences of 2.5, 5, 10, or 25 J/cm2, then allowed to recover for 3 to 24 hours. Control HCEnCi did not receive UV-A. Reactive oxygen species (ROS) were measured using H2DCFDA. Cell cytotoxicity was evaluated by lactate dehydrogenase (LDH) release...
April 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27078008/fuchs-endothelial-corneal-dystrophy-a-systematic-immunofluorescence-study-of-collagen-type-viii-suggests-heterogeneous-pathophysiology
#16
Esben Nielsen, Kim Nielsen, Anders Ivarsen, Nicholas S Greenhill, Paul F Davis, Jesper Hjortdal
PURPOSE: The purpose of this study was to investigate the pathophysiologic heterogeneity of Fuchs endothelial corneal dystrophy (FECD). METHODS: We conducted a systematic immunofluorescence study on 39 Descemet membrane samples from FECD patients and compared these with 10 Descemet membrane samples from patients with pseudophakic bullous keratopathy (PBK) and 7 normal corneas. Samples were analyzed with immunofluorescence using antibodies to the α1-chain [collagen VIII α1-chain (COL8A1)] and α2-chain (COL8A2)...
June 2016: Cornea
https://www.readbyqxmd.com/read/27055221/evaluation-of-endothelial-pump-function-in-fuchs-endothelial-dystrophy-before-and-after-endothelial-keratoplasty
#17
Esben Nielsen, Anders Ivarsen, Mogens Erlandsen, Jesper Hjortdal
PURPOSE: To evaluate the endothelial pump function in vivo after Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: In a prospective controlled trial, a group of 17 patients with Fuchs endothelial corneal dystrophy (FECD) eligible for DSAEK surgery and a group of 15 patients with cataract but with normal corneas eligible for cataract surgery (controls) were formed. A low oxygen-permeable contact lens was used to induce corneal edema. Changes in central corneal thickness were monitored as an indirect measure of endothelial cell pump function...
June 2016: Cornea
https://www.readbyqxmd.com/read/26980551/the-ultrastructures-and-mechanical-properties-of-the-descement-s-membrane-in-fuchs-endothelial-corneal-dystrophy
#18
Dan Xia, Shuai Zhang, Esben Nielsen, Anders Ramløv Ivarsen, Chunyong Liang, Qiang Li, Karen Thomsen, Jesper Østergaard Hjortdal, Mingdong Dong
Fuchs endothelial corneal dystrophy (FECD), is the most common corneal endothelial dystrophy, and contributes up to 50% of all corneal transplantations performed in developed countries. FECD develops in Descemet's membrane (DM) and possibly alters the mechanical properties and internal structures in this basal lamina. In this work, the morphology and mechanical properties of FECD-DMs are studied by transmission electron microscopy (TEM) and quantitative dynamic atomic force microscopy (QD-AFM) at nano scale...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26958853/wound-induced-polyploidization-regulation-by-hippo-and-jnk-signaling-and-conservation-in-mammals
#19
Vicki P Losick, Albert S Jun, Allan C Spradling
Tissue integrity and homeostasis often rely on the proliferation of stem cells or differentiated cells to replace lost, aged, or damaged cells. Recently, we described an alternative source of cell replacement- the expansion of resident, non-dividing diploid cells by wound-induced polyploidization (WIP). Here we show that the magnitude of WIP is proportional to the extent of cell loss using a new semi-automated assay with single cell resolution. Hippo and JNK signaling regulate WIP; unexpectedly however, JNK signaling through AP-1 limits rather than stimulates the level of Yki activation and polyploidization in the Drosophila epidermis...
2016: PloS One
https://www.readbyqxmd.com/read/26937169/fuchs-endothelial-corneal-dystrophy-current-perspectives
#20
REVIEW
Gustavo Vedana, Guadalupe Villarreal, Albert S Jun
Fuchs endothelial corneal dystrophy (FECD) is the most common corneal dystrophy and frequently results in vision loss. Hallmarks of the disease include loss of corneal endothelial cells and formation of excrescences of Descemet's membrane. Later stages involve all layers of the cornea. Impairment of endothelial barrier and pump function and cell death from oxidative and unfolded protein stress contribute to disease progression. The genetic basis of FECD includes numerous genes and chromosomal loci, although alterations in the transcription factor 4 gene are associated with the majority of cases...
2016: Clinical Ophthalmology
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