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https://www.readbyqxmd.com/read/27912189/genotoxicity-assessment-of-pulp-and-paper-mill-effluent-before-and-after-bacterial-degradation-using-allium-cepa-test
#1
Izharul Haq, Sharad Kumar, Abhay Raj, Mohtashim Lohani, G N V Satyanarayana
A lignin peroxidases-producing Serratia liquefaciens was used for bioremediation of pulp and paper (P&P) mill effluent. The treatment led to reduction of chemical oxygen demand (COD), colour, lignin and phenolic content by 84%, 72%, 61% and 95%, respectively. The effluent detoxification was studied by genotoxicity assays using Allium cepa L. (onion) root tip cells. Genotoxicity studies included measuring mitotic index (MI), chromosomal aberrations (CA) and nuclear abnormalities (NA) in root tip cells following treatment with 25, 50, 75 and 100% (v/v) of effluent...
November 29, 2016: Chemosphere
https://www.readbyqxmd.com/read/27903420/microbiology-of-processed-edible-insect-products-results-of-a-preliminary-survey
#2
Nils Th Grabowski, Günter Klein
Little is known of the microbiology of processed insect products. The present survey analysed a total of n=38 samples of deep-fried and spiced (Acheta domesticus, Locusta migratoria, and Omphisa fuscidentalis), cooked in soy sauce ("tsukudani"; Oxya yezoensis, Vespula flaviceps, and Bombyx mori), dried (A. domesticus, L. migatoria, Alphitobius diaperinus, Tenebrio molitor, B. mori, Hermetia illucens, and Musca domestica), powdered (H. illucens, T. molitor) and other (incl. deep-frozen B. mori and honeybee pollen) insect products microbiologically (total bacterial count [TBC], Enterobacteriaceae, staphylococci, bacilli, and yeasts and moulds counts, salmonellae, Listeria monocytogenes, and Escherichia coli)...
November 9, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27902872/happens-in-the-best-of-subfamilies-establishment-and-repeated-replacements-of-co-obligate-secondary-endosymbionts-within-lachninae-aphids
#3
Alejandro Manzano-Marín, Gitta Szabo, Jean-Christophe Simon, Matthias Horn, Amparo Latorre
Virtually all aphids maintain an obligate mutualistic symbiosis with bacteria from the Buchnera genus, which produce essential nutrients for their aphid hosts. Most aphids from the Lachninae subfamily have been consistently found to house additional endosymbionts, mainly Serratia symbiotica. This apparent dependence on secondary endosymbionts was proposed to have been triggered by the loss of the riboflavin biosynthetic capability by Buchnera in the Lachninae last common ancestor. However, an integral large-scale analysis of secondary endosymbionts in the Lachninae is still missing, hampering the interpretation of the evolutionary and genomic analyses of these endosymbionts...
November 30, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27889528/tailoring-shape-and-size-of-biogenic-silver-nanoparticles-to-enhance-antimicrobial-efficacy-against-mdr-bacteria
#4
Madhuree Kumari, Shipra Pandey, Ved Prakash Giri, Arpita Bhattacharya, Richa Shukla, Aradhana Mishra, C S Nautiyal
Spherical, rectangular, penta, and hexagonal silver nanoparticles of different dimensions were biosynthesized in an eco-friendly manner by biocontrol agent, Trichoderma viride by manipulating physical parameters, pH, temperature, and reaction time. The particles were characterized by UV-vis spectroscopy; Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) and Fourier Transform Infra-red Spectroscopy (FTIR). Shape and size dependent antimicrobial activity of nanoparticles against human pathogens was observed...
November 23, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27889464/enterocyte-purge-and-rapid-recovery-is-a-resilience-reaction-of-the-gut-epithelium-to-pore-forming-toxin-attack
#5
Kwang-Zin Lee, Matthieu Lestradet, Catherine Socha, Stefanie Schirmeier, Antonin Schmitz, Caroline Spenlé, Olivier Lefebvre, Céline Keime, Wennida M Yamba, Richard Bou Aoun, Samuel Liegeois, Yannick Schwab, Patricia Simon-Assmann, Frédéric Dalle, Dominique Ferrandon
Besides digesting nutrients, the gut protects the host against invasion by pathogens. Enterocytes may be subjected to damage by both microbial and host defensive responses, causing their death. Here, we report a rapid epithelial response that alleviates infection stress and protects the enterocytes from the action of microbial virulence factors. Intestinal epithelia exposed to hemolysin, a pore-forming toxin secreted by Serratia marcescens, undergo an evolutionarily conserved process of thinning followed by the recovery of their initial thickness within a few hours...
November 12, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/27876693/a-case-of-septic-shock-due-to-serratia-marcescens-pyelonephritis-and-bacteremia-in-a-patient-receiving-empagliflozin
#6
Wesley D Kufel, Ali Scrimenti, Jeffrey M Steele
Sodium-glucose cotransporter 2 (SGLT2) inhibitors have been associated with serious urinary tract infections (UTIs) including pyelonephritis and urosepsis. The Food and Drug Administration (FDA) issued a label change to include this warning in December 2015 due to a small number of cases (n = 19) reported to the FDA Adverse Event Reporting System. Details of these cases are limited and none involved empagliflozin. To date, there has been no published literature comprehensively describing serious UTIs attributed to empagliflozin...
November 21, 2016: Journal of Pharmacy Practice
https://www.readbyqxmd.com/read/27870556/twenty-species-of-hypobarophilic-bacteria-recovered-from-diverse-soils-exhibit-growth-under-simulated-martian-conditions-at-0-7%C3%A2-kpa
#7
Andrew C Schuerger, Wayne L Nicholson
Bacterial growth at low pressure is a new research area with implications for predicting microbial activity in clouds and the bulk atmosphere on Earth and for modeling the forward contamination of planetary surfaces like Mars. Here, we describe experiments on the recovery and identification of 20 species of bacterial hypobarophiles (def., growth under hypobaric conditions of approximately 1-2 kPa) in 10 genera capable of growth at 0.7 kPa. Hypobarophilic bacteria, but not archaea or fungi, were recovered from diverse soils, and high numbers of hypobarophiles were recovered from Arctic and Siberian permafrost soils...
November 21, 2016: Astrobiology
https://www.readbyqxmd.com/read/27867010/quorum-sensing-controls-adaptive-immunity-through-the-regulation-of-multiple-crispr-cas-systems
#8
Adrian G Patterson, Simon A Jackson, Corinda Taylor, Gary B Evans, George P C Salmond, Rita Przybilski, Raymond H J Staals, Peter C Fineran
Bacteria commonly exist in high cell density populations, making them prone to viral predation and horizontal gene transfer (HGT) through transformation and conjugation. To combat these invaders, bacteria possess an arsenal of defenses, such as CRISPR-Cas adaptive immunity. Many bacterial populations coordinate their behavior as cell density increases, using quorum sensing (QS) signaling. In this study, we demonstrate that QS regulation results in increased expression of the type I-E, I-F, and III-A CRISPR-Cas systems in Serratia cells in high-density populations...
November 16, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27865245/adolescent-with-recurrent-tuberculosis-can-it-be-chronic-granulomatous-disease
#9
Yashashree Gupta, Ira Shah
Chronic granulomatous disease (CGD) is an inherited primary immunodeficiency disorder with recurrent bacterial and fungal infections like Staphylococcus aureus, Nocardia spp, Serratia marcescens, Burkholderia cepacia, Salmonella spp. and Aspergillus species. We present a 13-year-old male child who had 3 episodes of tuberculosis (TB) at 5 years, 8 years and 13 years of age, respectively, with no other intercurrent infections and who was diagnosed as CGD at the age of 13 years. This case highlights the possibility of phenotypic variations of CGD...
July 2016: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/27862540/buchnera-has-changed-flatmate-but-the-repeated-replacement-of-co-obligate-symbionts-is-not-associated-with-the-ecological-expansions-of-their-aphid-hosts
#10
A S Meseguer, A Manzano-Marín, A Coeur d'Acier, A-L Clamens, M Godefroid, E Jousselin
Symbiotic associations with bacteria have facilitated important evolutionary transitions in insects and resulted in long-term obligate interactions. Recent evidence suggests that these associations are not always evolutionarily stable and that symbiont replacement and/or supplementation of an obligate symbiosis by an additional bacterium has occurred during the history of many insect groups. Yet, the factors favoring one symbiont over another in this evolutionary dynamic are not well understood; progress has been hindered by our incomplete understanding of the distribution of symbionts across phylogenetic and ecological contexts...
November 14, 2016: Molecular Ecology
https://www.readbyqxmd.com/read/27852235/enrichment-dynamics-of-listeria-monocytogenes-and-the-associated-microbiome-from-naturally-contaminated-ice-cream-linked-to-a-listeriosis-outbreak
#11
Andrea Ottesen, Padmini Ramachandran, Elizabeth Reed, James R White, Nur Hasan, Poorani Subramanian, Gina Ryan, Karen Jarvis, Christopher Grim, Ninalynn Daquiqan, Darcy Hanes, Marc Allard, Rita Colwell, Eric Brown, Yi Chen
BACKGROUND: Microbiota that co-enrich during efforts to recover pathogens from foodborne outbreaks interfere with efficient detection and recovery. Here, dynamics of co-enriching microbiota during recovery of Listeria monocytogenes from naturally contaminated ice cream samples linked to an outbreak are described for three different initial enrichment formulations used by the Food and Drug Administration (FDA), the International Organization of Standardization (ISO), and the United States Department of Agriculture (USDA)...
November 16, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27849602/synergistic-reaction-of-silver-nitrate-silver-nanoparticles-and-methylene-blue-against-bacteria
#12
Runze Li, Jie Chen, Thomas C Cesario, Xin Wang, Joshua S Yuan, Peter M Rentzepis
In this paper we describe the antibacterial effect of methylene blue, MB, and silver nitrate reacting alone and in combination against five bacterial strains including Serratia marcescens and Escherichia coli bacteria. The data presented suggest that when the two components are combined and react together against bacteria, the effects can be up to three orders of magnitude greater than that of the sum of the two components reacting alone against bacteria. Analysis of the experimental data provides proof that a synergistic mechanism is operative within a dose range when the two components react together, and additive when reacting alone against bacteria...
November 29, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27845476/are-liquids-an-efficient-vehicle-of-healthcare-associated-infections-a-review-of-reported-cases-in-italy-2000-2014
#13
D D'Alessandro, A Nusca, C Napoli
INTRODUCTION: In the field of healthcare-associated infections (HCAIs), one of the most reported, studied and discussed sources of infections is water, partly due to its controllability, but also because healthcare facilities, especially hospitals, require a significant quantity of water per day. In addition to water, during healthcare procedures, other liquids can serve as source of infections. The present study reports a review of those HCAIs associated to liquid vehicles occurred in Italy during the period 2000-2014...
November 2016: Annali di Igiene: Medicina Preventiva e di Comunità
https://www.readbyqxmd.com/read/27845335/an-iron-detection-system-determines-bacterial-swarming-initiation-and-biofilm-formation
#14
Chuan-Sheng Lin, Yu-Huan Tsai, Chih-Jung Chang, Shun-Fu Tseng, Tsung-Ru Wu, Chia-Chen Lu, Ting-Shu Wu, Jang-Jih Lu, Jim-Tong Horng, Jan Martel, David M Ojcius, Hsin-Chih Lai, John D Young
Iron availability affects swarming and biofilm formation in various bacterial species. However, how bacteria sense iron and coordinate swarming and biofilm formation remains unclear. Using Serratia marcescens as a model organism, we identify here a stage-specific iron-regulatory machinery comprising a two-component system (TCS) and the TCS-regulated iron chelator 2-isocyano-6,7-dihydroxycoumarin (ICDH-Coumarin) that directly senses and modulates environmental ferric iron (Fe(3+)) availability to determine swarming initiation and biofilm formation...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27844322/biomass-gas-exchange-and-nutrient-contents-in-upland-rice-plants-affected-by-application-forms-of-microorganism-growth-promoters
#15
Adriano Stephan Nascente, Marta Cristina Corsi de Filippi, Anna Cristina Lanna, Alan Carlos Alves de Souza, Valácia Lemes da Silva Lobo, Gisele Barata da Silva
Microorganisms are considered a genetic resource with great potential for achieving sustainable development of agricultural areas. The objective of this research was to determine the effect of microorganism application forms on the production of biomass, gas exchange, and nutrient content in upland rice. The experiment was conducted under greenhouse conditions in a completely randomized design in a factorial 7 × 3 + 1, with four replications. The treatments consisted of combining seven microorganisms with three application forms (microbiolized seed; microbiolized seed + soil drenched with a microorganism suspension at 7 and 15 days after sowing (DAS); and microbiolized seed + plant sprayed with a microorganism suspension at 7 and 15 DAS) and a control (water)...
November 14, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27833951/uncoupling-of-an-ammonia-channel-as-a-mechanism-of-allosteric-inhibition-in-anthranilate-synthase-of-serratia-marcescens-dynamic-and-graph-theoretical-analysis
#16
Ashutosh Srivastava, Somdatta Sinha
Anthranilate synthase (AS) is the first branch node enzyme that catalyzes the conversion of chorismate to anthranilate in the high energy-consuming tryptophan biosynthetic pathway in Serratia marcescens. AS, with an allosterically-bound inhibitor (tryptophan), shows complete inhibition in its catalytic function, but the inhibitor-bound structure is very similar to that of the substrate-bound AS. Even though the reaction mechanisms of several chorismate-utilizing enzymes are known, the unusual structure-function relationship in catalysis and allosteric inhibition of AS by tryptophan, with an insignificant change in structure, remains elusive...
November 11, 2016: Molecular BioSystems
https://www.readbyqxmd.com/read/27827894/phenanthrene-and-pyrene-modify-the-composition-and-structure-of-the-cultivable-endophytic-bacterial-community-in-ryegrass-lolium-multiflorum-lam
#17
Xuezhu Zhu, Li Jin, Kai Sun, Shuang Li, Xuelin Li, Wanting Ling
This study provides new insights into the dynamics of bacterial community structure during phytoremediation. The communities of cultivable autochthonous endophytic bacteria in ryegrass exposed to polycyclic aromatic hydrocarbons (PAHs) were investigated with regard to their potential to biodegrade PAHs. Bacterial counts and 16S rRNA gene sequence were used in the microbiological evaluation. A total of 33 endophytic bacterial strains were isolated from ryegrass plants, which represented 15 different genera and eight different classes, respectively...
November 3, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27825485/growth-promotion-of-lactuca-sativa-in-response-to-volatile-organic-compounds-emitted-from-diverse-bacterial-species
#18
Paola Fincheira, Herbert Venthur, Ana Mutis, Maribel Parada, Andrés Quiroz
Agrochemicals are currently used in horticulture to increase crop production. Nevertheless, their indiscriminate use is a relevant issue for environmental and legal aspects. Alternative tools for reducing fertilizers and synthetic phytohormones are being investigated, such as the use of volatile organic compounds (VOCs) as growth inducers. Some soil bacteria, such as Pseudomonas and Bacillus, stimulate Arabidopsis and tobacco growth by releasing VOCs, but their effects on vegetables have not been investigated...
December 2016: Microbiological Research
https://www.readbyqxmd.com/read/27816797/some-new-nano-sized-fe-ii-cd-ii-and-zn-ii-schiff-base-complexes-as-precursor-for-metal-oxides-sonochemical-synthesis-characterization-dna-interaction-in-vitro-antimicrobial-and-anticancer-activities
#19
Laila H Abdel-Rahman, Ahmed M Abu-Dief, Rafat M El-Khatib, Shimaa Mahdy Abdel-Fatah
The complexes of Fe(II), Cd(II) and Zn(II) with Schiff base derived from 2-amino-3-hydroxypyridine and 3-methoxysalicylaldehyde have been prepared. Melting points, decomposition temperatures, Elemental analyses, TGA, conductance measurements, infrared (IR) and UV-Visible spectrophotometric studies were utilized in characterizing the compounds. The UV-Visible spectrophotometric analysis revealed 1:1 (metal-ligand) stoichiometry for the three complexes. In addition to, the prepared complexes have been used as precursors for preparing their corresponding metal oxides nanoparticles via thermal decomposition...
November 1, 2016: Bioorganic Chemistry
https://www.readbyqxmd.com/read/27809759/analysis-of-the-genomic-sequences-and-metabolites-of-serratia-surfactantfaciens-sp-nov-yd25-t-that-simultaneously-produces-prodigiosin-and-serrawettin-w2
#20
Chun Su, Zhaoju Xiang, Yibo Liu, Xinqing Zhao, Yan Sun, Zhi Li, Lijun Li, Fan Chang, Tianjun Chen, Xinrong Wen, Yidan Zhou, Furong Zhao
BACKGROUND: Gram-negative bacteria of the genus Serratia are potential producers of many useful secondary metabolites, such as prodigiosin and serrawettins, which have potential applications in environmental bioremediation or in the pharmaceutical industry. Several Serratia strains produce prodigiosin and serrawettin W1 as the main bioactive compounds, and the biosynthetic pathways are co-regulated by quorum sensing (QS). In contrast, the Serratia strain, which can simultaneously produce prodigiosin and serrawettin W2, has not been reported...
November 3, 2016: BMC Genomics
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