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https://www.readbyqxmd.com/read/28445009/evaluation-of-six-phenotypic-methods-for-the-detection-of-carbapenemases-in-gram-negative-bacteria-with-characterized-resistance-mechanisms
#1
Kunling Sun, Xiuyu Xu, Jinrong Yan, Liping Zhang
BACKGROUND: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. METHODS: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444972/production-and-application-of-a-thermostable-lipase-from-serratia-marcescens-in-detergent-formulation-and-biodiesel-production
#2
Edgar Edurman García-Silvera, Fernando Martínez-Morales, Brandt Bertrand, Daniel Morales-Guzman, Nashbly Sarela Rosas-Galván, Renato León-Rodríguez, María Del Refugio Trejo-Hernández
In this study, extracellular lipase was produced by Serratia marcescens wild type and three mutant strains. The maximum lipase activity (80 U/mL) was obtained with the SMRG4 mutant strain using soybean oil. Using a 2(2) factorial design the lipase production increased 1.55-fold (124 U/mL) with 4% and 0.05 % soybean oil and Triton X-100, respectively. The optimum conditions for maximum lipase activity were 50°C and pH 8. However, the enzyme was active in a broad range of pH (6-10) and temperatures (5-55°C)...
April 26, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28436010/serratia-marcescens-an-italian-story
#3
Gianluca Nazzaro, Stefano Veraldi
No abstract text is available yet for this article.
April 23, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/28432358/differential-sensitivity-to-infections-and-antimicrobial-peptide-mediated-immune-response-in-four-silkworm-strains-with-different-geographical-origin
#4
Ottavia Romoli, Alessio Saviane, Andrea Bozzato, Paola D'Antona, Gianluca Tettamanti, Andrea Squartini, Silvia Cappellozza, Federica Sandrelli
The domesticated silkworm Bombyx mori has an innate immune system, whose main effectors are the antimicrobial peptides (AMPs). Silkworm strains are commonly grouped into four geographical types (Japanese, Chinese, European and Tropical) and are generally characterised by a variable susceptibility to infections. To clarify the genetic and molecular mechanisms on which the different responses to infections are based, we exposed one silkworm strain for each geographical area to oral infections with the silkworm pathogens Enterococcus mundtii or Serratia marcescens...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28429335/imipenem-resistance-in-serratia-marcescens-is-mediated-by-plasmid-expression-of-kpc-2
#5
W-Q Su, Y-Q Zhu, N-M Deng, L Li
OBJECTIVE: Imipenem is a broad-spectrum carbapenem antibiotic with applications against severe bacterial infections. Here, we describe the identification of imipenem-resistant Serratia marcescens in our hospital and the role of plasmid-mediated KPC-2 expression in imipenem resistance. MATERIALS AND METHODS: We used the modified Hodge test to detect carbapenemase produced in imipenem-resistant strains. RESULTS: His resistance can be transferred to E...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28424942/serratia-bozhouensis-sp-nov-isolated-from-sewage-samples-of-a-dairy-farm
#6
Fei Shang, Ting Xue, Man Wang, Xiaolin Chen, Li Yu, Ming Zhang
A Gram-negative, rod-shaped, salt-tolerant, non-pigmented, and non-spore-forming bacterium, designated strain W1(T) (type strain CICC 23797 = CGMCC1.14949), was isolated from sewage samples of a dairy farm in Bozhou, Anhui, China. Strain W1 was resistant to lincomycin, troleandomycin, rifamycin, and vancomycin. Sequence analysis of the 16S rDNA gene revealed that the strain showed sequence similarity of 98.2% with the closest related species Serratia quinivorans CP6a(T). The genomic DNA G+C content of the isolate was 52...
April 20, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28421038/bacteriophages-against-serratia-as-fish-spoilage-control-technology
#7
Igor Hernández
Bacteria of the genus Serratia, mainly S. proteamaculans and S. fonticola, are important spoilage agents in Atlantic horse mackerel (Trachurus trachurus). In order to evaluate whether bacteriophages against Serratia could delay the spoilage process, 11 viral strains active against this genus were isolated from food and best candidate was applied to fresh mackerel filets. All the phages belong to the Siphoviridae and Podoviridae families and were active at multiplicity of infection (MOI) levels below 1:1 in Long & Hammer broth...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28419869/allodemic-distribution-of-plasmids-co-harbouring-blactx-m-15-aac-6-ib-cr-qnrb-in-klebsiella-pneumoniae-is-the-main-source-of-extended-spectrum-%C3%AE-lactamases-in-uruguay-s-paediatric-hospital
#8
V Garcia-Fulgueiras, L Araujo, I Bado, N F Cordeiro, M I Mota, G Laguna, G Algorta, R Vignoli
OBJECTIVES: This study aimed to describe the characteristics of clinical isolates of extended-spectrum β-lactamase (ESBL)-producing enterobacteria (EPE) in Uruguay's paediatric hospital. METHODS: ESBLs, qnr alleles and aac(6')-Ib-cr were sought and characterised in EPE isolated between March 2010 and March 2012. Transfer of resistance determinants was assessed by conjugation. Incompatibility (Inc) groups, plasmid toxin-antitoxin systems (TAS) and plasmid size were determined in transconjugants...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28411217/transfer-potential-of-plasmids-encoding-extended-spectrum-cephalosporin-resistance-in-escherichia-coli-from-poultry
#9
Solveig Sølverød Mo, Marianne Sunde, Hanna Karin Ilag, Solveig Langsrud, Even Heir
Escherichia coli resistant to extended-spectrum cephalosporins (ESC) are widely distributed in Norwegian broiler production, and the majority harbour transferable IncK or IncI1 plasmids with blaCMY-2 Persistent occurrence in broiler farms may occur through survival of ESC-resistant E. coli in the farm environment, or by transfer and maintenance of resistance plasmids within a population of environmental bacteria with high survival abilities. The aim of this study was to determine the transferability of two successful blaCMY-2 carrying plasmids belonging to the incompatibility groups IncK and IncI1, into E...
April 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28408760/fungal-volatile-compounds-induce-production-of-the-secondary-metabolite-sodorifen-in-serratia-plymuthica-pri-2c
#10
Ruth Schmidt, Victor de Jager, Daniela Zühlke, Christian Wolff, Jörg Bernhardt, Katarina Cankar, Jules Beekwilder, Wilfred van Ijcken, Frank Sleutels, Wietse de Boer, Katharina Riedel, Paolina Garbeva
The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics analyses of Serratia plymuthica PRI-2C exposed to VOCs emitted by the fungal pathogen Fusarium culmorum. We find that the bacterium responds to fungal VOCs with changes in gene and protein expression related to motility, signal transduction, energy metabolism, cell envelope biogenesis, and secondary metabolite production...
April 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28406998/structure-and-dynamics-of-the-gut-bacterial-microbiota-of-the-bark-beetle-dendroctonus-rhizophagus-curculionidae-scolytinae-across-their-life-stages
#11
Carlos Iván Briones-Roblero, Juan Alfredo Hernández-García, Roman Gonzalez-Escobedo, L Viridiana Soto-Robles, Flor N Rivera-Orduña, Gerardo Zúñiga
Bark beetles play an important role as agents of natural renovation and regeneration in coniferous forests. Several studies have documented the metabolic capacity of bacteria associated with the gut, body surface, and oral secretions of these insects; however, little is known about how the bacterial community structure changes during the life cycle of the beetles. This study represents the first comprehensive analysis of the bacterial community of the gut of the bark beetle D. rhizophagus during the insect's life cycle using 454 pyrosequencing...
2017: PloS One
https://www.readbyqxmd.com/read/28404510/effects-of-fe-ni-and-fe-ni-metallic-nanoparticles-on-power-production-and-biosurfactant-production-from-used-vegetable-oil-in-the-anode-chamber-of-a-microbial-fuel-cell
#12
Jia Liu, Cumaraswamy Vipulanandan
In this study, metallic nanoparticles (Fe, Ni, and Fe/Ni) were used as cathode catalysts to enhance power production and to improve the anode performance of a two-chambered microbial fuel cell (MFC). The metallic nanoparticles were rod-shaped and produced by the precipitation/co-precipitation method. A biosurfactant was produced in the anode chamber of the MFC from used vegetable oil by the bacteria Serratia sp. Overall cell voltage, power density, bacterial growth, and biosurfactant production were studied by applying different types of metallic nanoparticles to the cathode electrode...
April 9, 2017: Waste Management
https://www.readbyqxmd.com/read/28400020/bacterial-communities-of-fresh-goat-meat-packaged-in-modified-atmosphere
#13
Elia Carrizosa, María José Benito, Santiago Ruiz-Moyano, Alejandro Hernández, Maria Del Carmen Villalobos, Alberto Martín, María de Guía Córdoba
The objective of this work was to study the growth and development of fortuitous flora and food pathogens in fresh goat meat packaged under modified atmospheres containing two different concentrations of CO2. Meat samples were stored at 10 °C under two different modified-atmosphere packing (MAP) conditions: treatment A had 45% CO2 + 20% O2 + 35% N2 and treatment B had 20% CO2 + 55% O2 + 25% N2. During 14 days of storage, counts of each bacterial group and dominant species identification by 16S rRNA gene sequencing were performed to determine the microbial diversity present...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28397557/factors-associated-with-infectious-laryngitis-a-retrospective-review-of-15-cases
#14
Carissa M Thomas, Marie E Jetté, Matthew S Clary
OBJECTIVES: To identify the culturable microbes associated with infectious laryngitis and outline effective treatment strategies. METHODS: This is a retrospective chart review of adult patients with persistent dysphonia plus evidence of laryngeal inflammation who underwent biopsy for culture at a tertiary care medical center. Demographic factors, symptoms as reported on validated patient assessment tools, past medical history, social history, culture results, and treatment duration and response were reviewed...
May 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/28387756/pore-forming-toxin-mediated-ion-dysregulation-leads-to-death-receptor-independent-necroptosis-of-lung-epithelial-cells-during-bacterial-pneumonia
#15
Norberto González-Juarbe, Kelley Margaret Bradley, Anukul Taranath Shenoy, Ryan Paul Gilley, Luis Felipe Reyes, Cecilia Anahí Hinojosa, Marcos Ignacio Restrepo, Peter Herman Dube, Molly Ann Bergman, Carlos Javier Orihuela
We report that pore-forming toxins (PFTs) induce respiratory epithelial cell necroptosis independently of death receptor signaling during bacterial pneumonia. Instead, necroptosis was activated as a result of ion dysregulation arising from membrane permeabilization. PFT-induced necroptosis required RIP1, RIP3 and MLKL, and could be induced in the absence or inhibition of TNFR1, TNFR2 and TLR4 signaling. We detected activated MLKL in the lungs from mice and nonhuman primates experiencing Serratia marcescens and Streptococcus pneumoniae pneumonia, respectively...
April 7, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28385486/analysis-and-optimization-of-process-parameters-for-production-of-polyhydroxyalkanoates-along-with-wastewater-treatment-by-serratia-sp-istvkr1
#16
Asmita Gupta, Madan Kumar, Indu Shekhar Thakur
A previously reported biodegrading bacterial strain Serratia sp. ISTVKR1 was studied for polyhydroxyalkanoate (PHA) production along with wastewater contaminant removal. Nile red fluorescence, GC-MS, FT-IR, NMR and TEM confirmed the accumulation of homopolymer poly-3-hydroxyvalerate (PHV) within the bacterial cells. Analysis of culture after 72h of bacterial treatment showed maximum COD removal (8.4-fold), non-detection of organic contaminants such as 1H-Cyclopropa [a] naphthalene (R.T.=10.12) using GC-MS and increased proportion of elements like Cr, Mn, Fe, Ni, Cu, Cd and Pb in the bacterial cell pellets by SEM-EDX analysis...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28382701/bacteria-can-proliferate-in-thawed-cryoprecipitate-stored-at-room-temperature-for-longer-than-4%C3%A2-h
#17
S Ramirez-Arcos, C Jenkins, W P Sheffield
Although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24 h at ambient temperature, several jurisdictions limit such storage to 4-6 h. Here, we separately spiked thawed cryoprecipitate units with four bacterial strains: Staphylococcus epidermidis, Serratia liquefaciens, Pseudomonas putida and Pseudomonas aeruginosa. No strains grew in the first 4 h of storage, but by 24 h, three of four exhibited up to 1000-fold proliferation. Pathogen inactivation technologies could be explored to mitigate the safety risk posed by extending storage of thawed cryoprecipitate at room temperature...
April 6, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28377759/comparative-genomic-analysis-reveals-organization-function-and-evolution-of-ars-genes-in-pantoea-spp
#18
Liying Wang, Jin Wang, Chuanyong Jing
Numerous genes are involved in various strategies to resist toxic arsenic (As). However, the As resistance strategy in genus Pantoea is poorly understood. In this study, a comparative genome analysis of 23 Pantoea genomes was conducted. Two vertical genetic arsC-like genes without any contribution to As resistance were found to exist in the 23 Pantoea strains. Besides the two arsC-like genes, As resistance gene clusters arsRBC or arsRBCH were found in 15 Pantoea genomes. These ars clusters were found to be acquired by horizontal gene transfer (HGT) from sources related to Franconibacter helveticus, Serratia marcescens, and Citrobacter freundii...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28376361/bioelectrochemical-treatment-of-acid-mine-drainage-amd-from-an-abandoned-coal-mine-under-aerobic-condition
#19
Meisam Peiravi, Shekhar R Mote, Manoj K Mohanty, Jia Liu
In this study, a bioelectrochemical system (BES) was used to treat acid mine drainage (AMD) from an abandoned coal mine in the cathode chamber under aerobic condition. Activated sludge from a local wastewater treatment plant was used in the anode chamber of the BES to supply electrons to the treatment. After 7days, the pH of the cathode solution enhanced from 2.5 to 7.3. More than 99% of Al, Fe and Pb were removed, and removal rates of 93%, 91%, 89% and 69% were achieved for Cd, Zn, Mn and Co respectively with biocathode...
March 22, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28373145/characterization-of-biocontrol-bacterial-strains-isolated-from-a-suppressiveness-induced-soil-after-amendment-with-composted-almond-shells
#20
Carmen Vida, Francisco M Cazorla, Antonio de Vicente
The improvement in soil quality of avocado crops through organic amendments with composted almond shells has a positive effect on crop yield and plant health, and enhances soil suppressiveness against the phytopathogenic fungus Rosellinia necatrix. In previous studies, induced soil suppressiveness against this pathogen was related to stimulation of Gammaproteobacteria, especially some members of Pseudomonas spp. with biocontrol-related activities. In this work, we isolated bacteria from this suppressiveness-induced amended soil using a selective medium for Pseudomonas-like microorganisms...
April 1, 2017: Research in Microbiology
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