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https://www.readbyqxmd.com/read/27913878/piroplasms-in-brown-hyaenas-parahyaena-brunnea-and-spotted-hyaenas-crocuta-crocuta-in-namibia-and-south-africa-are-closely-related-to-babesia-lengau
#1
Richard E J Burroughs, Barend L Penzhorn, Ingrid Wiesel, Nancy Barker, Ilse Vorster, Marinda C Oosthuizen
The objective of our study was identification and molecular characterization of piroplasms and rickettsias occurring in brown (Parahyaena brunnea) and spotted hyaenas (Crocuta crocuta) from various localities in Namibia and South Africa. Whole blood (n = 59) and skin (n = 3) specimens from brown (n = 15) and spotted hyaenas (n = 47) were screened for the presence of Babesia, Theileria, Ehrlichia and Anaplasma species using the reverse line blot (RLB) hybridization technique. PCR products of 52/62 (83...
December 2, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27908772/anaplasma-platys-in-dogs-from-uruguay
#2
Luis Carvalho, Maria Teresa Armua-Fernandez, Nicolás Sosa, María Laura Félix, José Manuel Venzal
Anaplasmataceae family members include vector-borne bacteria of veterinary importance that may also affect humans. Ehrlichia canis and Anaplasma platys are the main members of this family detected in dogs worldwide. In Uruguay there are not many published studies on tick-borne pathogens affecting dogs, the only haemoparasite molecularly confirmed in dogs, is the piroplasm Rangelia vitalii. The aim of the present work was to detect the presence of A. platys and E. canis in dogs and dogs-associated ticks of two localities in Northwestern Uruguay...
November 17, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27902186/multigenetic-characterization-of-candidatus-xenohaliotis-californiensis
#3
Francesco Cicala, James D Moore, Jorge Cáceres-Martínez, Miguel A Del Río-Portilla, Mónica Hernández-Rodríguez, Rebeca Vásquez-Yeomans, Axayácatl Rocha-Olivares
"Candidatus Xenohaliotis californiensis" (or Ca. Xc) is the etiological agent of the withering syndrome (WS), a chronic wasting disease affecting most if not all North American species of abalone, and has been described as a Rickettsiales-like prokaryote. Genetic data about this species is limited to the 16S rRNA gene. The inability to grow it axenically has hindered its genetic and genomic characterization and, in consequence, a thorough analysis of its systematics. Here, we amplified and sequenced five genes (16S rRNA, 23S rRNA, ftsZ, virD4, and virB11) of Ca...
October 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27894847/pcr-screening-of-tick-borne-agents-in-sensitive-conservation-areas-southeast-portugal
#4
Maria Margarida Santos-Silva, Pedro Melo, Nuno Santos, Sandra Antunes, Luís Raposo Duarte, Joana Ferrolho, Natacha Milhano, Patrícia Tavares Santos, Ana Domingos, Ana Sofia Santos
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs)...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27890889/first-molecular-detection-of-tick-borne-pathogens-in-dogs-from-jiangxi-china
#5
Weiqing Zheng, Mingming Liu, Paul Franck Adjou Moumouni, Xiaoqing Liu, Artemis Efstratiou, Zhanbin Liu, Yangqing Liu, Huiying Tao, Huanping Guo, Guanbo Wang, Yang Gao, Zifen Li, Aaron Edmund Ringo, Charoonluk Jirapattharasate, Haiying Chen, Xuenan Xuan
In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp...
November 26, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27876946/evaluation-of-clinical-biochemical-and-haematological-markers-in-natural-infection-of-canine-monocytic-ehrlichiosis
#6
Rahul Parashar, Vikrant Sudan, Amit Kumar Jaiswal, Ashish Srivastava, Daya Shanker
Caanine monocytic ehrlichiosis caused by Ehrlichia canis has gained wider significance owing to its potential to inflict significant deleterious effect on the health of companion animals. In the present study, 46 confirmed ehrlichiosis positive dogs were evaluated for the alterations in clinical, haematological and biochemical attributes. Depression, anorexia, pyrexia, anaemia, weakness, jaundice, melana, vomition and diarrhoea were the main clinical symptoms onserved. Haematological alterations included pancytopenia especially thrombocytopenia...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27865263/molecular-assessment-of-the-transplacental-transmission-of-toxoplasma-gondii-neospora-caninum-brucella-canis-and-ehrlichia-canis-in-dogs
#7
Isis Indaiara Gonçalves Granjeiro Taques, Tatiane Rodrigues Barbosa, Andresa de Cássia Martini, Letícia Camara Pitchenin, Ísis Assis Braga, Andréia Lima Tomé de Melo, Luciano Nakazato, Valéria Dutra, Daniel Moura de Aguiar
Given the fact that numerous microbial species can be detected in pregnant female dogs, the objective of this study was to assess the transplacental transmission of Brucella canis, Ehrlichia canis, Neospora caninum and Toxoplasma gondii in stillborn puppies. This study involved 41 stillborn puppies, 78.6% of which were positive for T. gondii, 52.4% for N. caninum and 59.5% for B. canis. E. canis was not detected in any of the analyzed puppies. Pregnancy is an important physiological condition for the transmission of infectious agents to puppies and transplacental transmission may be epidemiologically relevant in the spread of these opportunistic agents...
December 2016: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/27852813/initial-development-and-preliminary-evaluation-of-a-multiplex-bead-assay-to-detect-antibodies-to-ehrlichia-canis-anaplasma-platys-and-ehrlichia-chaffeensis-outer-membrane-peptides-in-naturally-infected-dogs-from-grenada-west-indies
#8
Melinda J Wilkerson, Kelley E Black, Marta Lanza-Perea, Bhumika Sharma, Kathryn Gibson, Diana M Stone, Anushka George, Arathy D S Nair, Roman R Ganta
Tick-borne bacteria, Ehrlichia canis, Anaplasma platys, and Ehrlichia chaffeensis are significant pathogens of dogs worldwide, and coinfections of E. canis and A. platys are common in dogs on the Caribbean islands. We developed and evaluated the performance of a multiplex bead-based assay to detect antibodies to E. canis, A. platys, and E. chaffeensis peptides in dogs from Grenada, West Indies, where E. canis and A. platys infections are endemic. Peptides from outer membrane proteins of P30 of E. canis, OMP-1X of A...
November 15, 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27825733/a-comparison-of-dna-extraction-protocols-from-blood-spotted-on-fta-cards-for-the-detection-of-tick-borne-pathogens-by-reverse-line-blot-hybridization
#9
Zerihun Hailemariam, Jabbar Sabir Ahmed, Peter-Henning Clausen, Ard Menzo Nijhof
An essential step in the molecular detection of tick-borne pathogens (TBPs) in blood is the extraction of DNA. When cooled storage of blood under field conditions prior to DNA extraction in a dedicated laboratory is not possible, the storage of blood on filter paper forms a promising alternative. We evaluated six DNA extraction methods from blood spotted on FTA Classic(®) cards (FTA cards), to determine the optimal protocol for the subsequent molecular detection of TBPs by PCR and the Reverse Line Blot hybridization assay (RLB)...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27825659/ferritin-1-silencing-effect-in-rhipicephalus-sanguineus-sensu-lato-acari-ixodidae-during-experimental-infection-with-ehrlichia-canis
#10
Joana Ferrolho, Sandra Antunes, Gustavo S Sanches, Joana Couto, Patrícia M Évora, Catarina Rosa, Marcos R André, Rosângela Z Machado, Gervásio H Bechara, Ana Domingos
Rhipicephalus sanguineus sensu lato (s.l.) is a very common ectoparasite of domestic dogs able to transmit several pathogens of human and veterinary importance. Tick infestations and tick-borne diseases (TBDs) remain a serious and persistent problem, due to the lack of efficient control measures. It is therefore vital that novel approaches to control are pursued. Whilst vaccination is recognised as a potential control method to reduce tick infestation, no anti-R. sanguineus vaccine is available. Ticks depend on their blood meals to obtain nutrients and to achieve sexual maturity, which exposes them to vast amounts of iron...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27822541/16s-rrna-amplicon-sequencing-for-epidemiological-surveys-of-bacteria-in-wildlife
#11
Maxime Galan, Maria Razzauti, Emilie Bard, Maria Bernard, Carine Brouat, Nathalie Charbonnel, Alexandre Dehne-Garcia, Anne Loiseau, Caroline Tatard, Lucie Tamisier, Muriel Vayssier-Taussat, Helene Vignes, Jean-François Cosson
The human impact on natural habitats is increasing the complexity of human-wildlife interactions and leading to the emergence of infectious diseases worldwide. Highly successful synanthropic wildlife species, such as rodents, will undoubtedly play an increasingly important role in transmitting zoonotic diseases. We investigated the potential for recent developments in 16S rRNA amplicon sequencing to facilitate the multiplexing of the large numbers of samples needed to improve our understanding of the risk of zoonotic disease transmission posed by urban rodents in West Africa...
July 2016: MSystems
https://www.readbyqxmd.com/read/27812828/bacteria-of-the-genera-ehrlichia-and-rickettsia-in-ticks-of-the-family-ixodidae-with-medical-importance-in-argentina
#12
Patrick S Sebastian, Evelina L Tarragona, María N Saracho Bottero, Atilio J Mangold, Ute Mackenstedt, Santiago Nava
The aim of this study was to get an overview about the occurrence of bacteria from the genus Ehrlichia and Rickettsia in ixodid ticks with medical importance in Argentina. Therefore, in 2013 and 2014, free-living ticks were collected in different provinces of northern Argentina. These ticks were determined as Amblyomma sculptum, Amblyomma neumanni, Amblyomma parvum, Amblyomma triste, Amblyomma ovale, Amblyomma tonelliae and Haemaphysalis juxtakochi. All samples were tested to determine the infection with Ehrlichia spp...
November 3, 2016: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/27810200/cardiac-troponin-i-concentrations-electrocardiographic-and-echocardiographic-variables-remained-unchanged-in-dogs-experimentally-infected-with-ehrlichia-canis
#13
Lamprini Kalogianni, Christos K Koutinas, Konstantina Theodorou, Panagiotis G Xenoulis, Jan S Suchodolski, Shimon Harrus, Joerg M Steiner, Victoria I Siarkou, Mathios E Mylonakis
Canine monocytic ehrlichiosis (CME, Ehrlichia canis) has occasionally been associated with myocardial injury. The aim of the present study was to serially measure and evaluate cardiac troponin I (cTnI) concentrations in dogs with experimentally induced acute and subclinical CME and to evaluate potential associations between cTnI concentration and an array of echocardiographic and electrocardiographic parameters. Serum cTnI concentration and simultaneous echocardiographic and electrocardiographic recordings were evaluated in 12 healthy Beagle dogs prior to experimental infection and on days 20 and 90 post-inoculation with E...
November 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27787221/critical-analysis-of-vector-borne-infections-in-dogs-babesia-vogeli-babesia-gibsoni-ehrlichia-canis-and-hepatozoon-canis-in-punjab-india
#14
Lachhman Das Singla, Deepak Sumbria, Ajay Mandhotra, M S Bal, Paramjit Kaur
There are few published studies on various vector borne diseases of dogs in India and most depict clinical infection in dogs, diagnosed by observation of the haemopathogens in stained blood smears. This study provides the first report regarding molecular confirmation and ancestral relationship analysis of blood smears positive cases of assorted haemopathogens in Punjab province of India. On blood smear examination, haemopathogens were observed in 124 out of 778 (15.95%, 95% CI: 13.53- 18.68) blood smears. Further polymerase chain reactions (PCR) was used on bloods smear positive cases to validate the results...
December 1, 2016: Acta Parasitologica
https://www.readbyqxmd.com/read/27773826/risk-of-encountering-ticks-and-tick-borne-pathogens-in-a-rapidly-growing-metropolitan-area-in-the-u-s-great-plains
#15
Bruce H Noden, Scott R Loss, Courtney Maichak, Faithful Williams
The prevalence of tick-borne diseases has increased dramatically in many urban areas of the U.S., yet little is known about the ecology of ticks and tick-borne pathogens in relation to characteristics of North American urban and suburban landscapes. This study aimed to begin identification of the risk of encountering ticks and tick-borne pathogens within a rapidly expanding metropolitan area in the U.S. Great Plains region. Ten sites across Oklahoma City, Oklahoma were selected for tick sampling based on presence of tick habitat and level of urbanization intensity...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27758794/severe-human-monocytic-ehrlichiosis-presenting-with-altered-mental-status-and-seizures
#16
Christian Geier, Jessica Davis, Marc Siegel
A previously healthy 66-year-old woman living in the Mid-Atlantic USA presented to the hospital with lethargy, ataxia and slurred speech. 2 weeks prior she had removed a tick from her right groin. She reported malaise, fevers, diarrhoea, cough and a rash. Physical examination revealed a maculopapular rash on her chest, and lung auscultation revealed bi-basilar rales. Laboratory tests were remarkable for hyponatraemia, leucopenia and thrombocytopenia. Chest X-ray demonstrated bilateral pleural effusions with pulmonary oedema...
October 6, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27747194/recombination-is-a-major-driving-force-of-genetic-diversity-in-the-anaplasmataceae-ehrlichia-ruminantium
#17
Nídia Cangi, Jonathan L Gordon, Laure Bournez, Valérie Pinarello, Rosalie Aprelon, Karine Huber, Thierry Lefrançois, Luís Neves, Damien F Meyer, Nathalie Vachiéry
The disease, Heartwater, caused by the Anaplasmataceae E. ruminantium, represents a major problem for tropical livestock and wild ruminants. Up to now, no effective vaccine has been available due to a limited cross protection of vaccinal strains on field strains and a high genetic diversity of Ehrlichia ruminantium within geographical locations. To address this issue, we inferred the genetic diversity and population structure of 194 E. ruminantium isolates circulating worldwide using Multilocus Sequence Typing based on lipA, lipB, secY, sodB, and sucA genes...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27729288/amblyomma-americanum-ticks-infected-with-in-vitro-cultured-wild-type-and-mutants-of-ehrlichia-chaffeensis-are-competent-to-produce-infection-in-na%C3%A3-ve-deer-and-dogs
#18
Deborah C Jaworski, Chuanmin Cheng, Arathy D S Nair, Roman R Ganta
Monocytic ehrlichiosis in people caused by the intracellular bacterium, Ehrlichia chaffeensis, is an emerging infectious disease transmitted by the lone star tick, Amblyomma americanum. Tick transmission disease models for ehrlichiosis require at least two hosts and two tick blood feeding episodes to recapitulate the natural transmission cycle. One blood feeding is necessary for the tick to acquire the infection from an infected host and the next feeding is needed to transmit the bacterium to a naïve host...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27720382/detection-of-candidatus-neoehrlichia-mikurensis-and-ehrlichia-muris-in-estonian-ticks
#19
Anna Ivanova, Julia Geller, Olga Katargina, Kairi Värv, Åke Lundkvist, Irina Golovljova
So far neglected bacteria like Candidatud Neoehrlichia mikurensis and Ehrlichia muris-like agents get increased attention in the recent past. Ixodid ticks were demonstrated to harbor both of these pathogens. Estonia is populated by two medically important tick species, I. ricinus and I. persulcatus. In this study the presence of E. muris and Candidatus N. mikurensis in these two tick species was investigated. Tick DNA was analyzed by nested PCR and subsequent sequencing for the presence of 16S rRNA of E. muris and Candidatus N...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27720381/borrelia-burgdorferi-dna-absent-multiple-rickettsia-spp-dna-present-in-ticks-collected-from-a-teaching-forest-in-north-central-florida
#20
Katherine Sayler, Jessica Rowland, Carisa Boyce, Emma Weeks
Tick-borne diseases are an emerging public health threat in the United States. In Florida, there has been public attention directed towards the possibility of locally acquired Borrelia burgdorferi sensu stricto, the causative agent of Lyme disease, in association with the lone star tick. The aim of this study was to determine the prevalence of ticks and the pathogens they carry and potentially transmit, such as B. burgdorferi, in a highly utilized teaching and research forest in North Central Florida. Ticks were collected by dragging and flagging methods over a four month period in early 2014, identified, and tested by PCR for multiple pathogens including Anaplasma, Borrelia, Rickettsia, and Ehrlichia species...
January 2017: Ticks and Tick-borne Diseases
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