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https://www.readbyqxmd.com/read/28197924/sumoylation-as-an-integral-mechanism-in-bacterial-infection-and-disease-progression
#1
Chittur V Srikanth, Smriti Verma
Post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. Among these, particularly the modification with Small Ubiquitin-like Modifiers (SUMO) is being recognized as a pathway crucial for cell homeostasis and health. Understandably, bacterial pathogens intervene with the SUMO pathway of the host for ensuring successful infection. Among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. Majority of them including Salmonella Typhimurium, Shigella flexneri and Listeria monocytogenes target the E2 conjugating enzyme Ubc9...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28188085/species-of-ticks-and-carried-pathogens-in-owned-dogs-in-spain-results-of-a-one-year-national-survey
#2
Agustín Estrada-Peña, Xavier Roura, Angel Sainz, Guadalupe Miró, Laia Solano-Gallego
This study presents the results of a national survey in Spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. In the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. The primary species of ticks were Rhipicephalus sanguineus s.l. (53%), Dermacentor reticulatus (9%), Ixodes ricinus (9%), and I. hexagonus (4%). Parasitism by two species of these ticks was rare...
February 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28143697/first-detection-of-tick-borne-pathogens-of-dogs-from-malta
#3
Elaine Licari, Nóra Takács, Norbert Solymosi, Róbert Farkas
The knowledge about the vector-borne infections in domestic dogs has been increasing worldwide. However no studies have been done on hard tick infestation and tick-borne pathogens (TBPs) of dogs present in Malta. Therefore, a total of 99 dogs was selected and inspected between March and July 2013 in 18 urban and 17 rural areas on the islands of Malta and Gozo. All ticks were removed from the dogs and identified. Blood samples were taken and tested for protozoa (Babesia spp. and Hepatozoon spp.) and bacteria (Anaplasma spp...
January 18, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28131594/detection-of-borrelia-miyamotoi-and-other-tick-borne-pathogens-in-human-clinical-specimens-and-ixodes-scapularis-ticks-in-new-york-state-2012-2015
#4
Danielle Wroblewski, Linda Gebhardt, Melissa A Prusinski, Lisa J Meehan, Tanya A Halse, Kimberlee A Musser
Borrelia miyamotoi (Bm) is a recently emerging bacterial agent transmitted by several species of ixodid ticks. Diagnosis of Bm infection can be challenging, as the organism is not easily cultivable. We have developed and validated a multiplex real-time PCR to simultaneously identify Bm infection and the agents causing human granulocytic anaplasmosis and human monocytic ehrlichiosis, Anaplasma phagocytophilum and Ehrlichia chaffeensis, respectively. The assay is 100% specific; highly sensitive, detecting 11 gene copies of Bm DNA in both whole blood and cerebral spinal fluid; and provides rapid results in less than two hours...
January 16, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28129781/experimental-evaluation-of-peromyscus-leucopus-as-a-reservoir-host-of-the-ehrlichia-muris-like-agent
#5
Geoffrey E Lynn, Jonathan D Oliver, Ingrid Cornax, M Gerard O'Sullivan, Ulrike G Munderloh
BACKGROUND: The Ehrlichia muris-like agent (EMLA) is a newly recognized human pathogen in the North Central United States. Although blacklegged ticks (Ixodes scapularis) have been identified as capable vectors, wild reservoirs have not yet been established for EMLA. As key hosts for I. scapularis, white-footed mice (Peromyscus leucopus) are important reservoirs for various tick-borne pathogens, and potentially, for EMLA. The objective of this study was to evaluate reservoir competence in P...
January 28, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28089651/canine-tick-borne-pathogens-in-cyprus-and-a-unique-canine-case-of-multiple-co-infections
#6
Charalampos Attipa, Chelsea A E Hicks, Emily N Barker, Vasiliki Christodoulou, Kyriaki Neofytou, Mathios E Mylonakis, Victoria I Siarkou, Elpida I Vingopoulou, Francesca Soutter, Dimosthenis Chochlakis, Anna Psaroulaki, Kostas Papasouliotis, Séverine Tasker
Canine tick-borne pathogens such as Ehrlichia canis and Hepatozoon canis are widespread in the Mediterranean basin but have never been reported or investigated in Cyprus. We describe herein the presence of canine tick-borne pathogens in three dogs with clinical signs compatible with vector-borne diseases from Paphos area of Cyprus. Molecular and phylogenetic analysis revealed the presence of E. canis, Anaplasma platys, H. canis, Babesia vogeli and Mycoplasma haemocanis in Cyprus. One dog co-infected with E...
December 26, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28055326/prevalence-and-distribution-of-human-and-tick-infections-with-the-ehrlichia-muris-like-agent-and-anaplasma-phagocytophilum-in-wisconsin-2009-2015
#7
Darby S Murphy, Xia Lee, Scott R Larson, Diep K Hoang Johnson, Theoren Loo, Susan M Paskewitz
Ehrlichiosis and anaplasmosis are important emerging tickborne zoonoses that affect both humans and animals. Knowledge of the geographic distribution and prevalence of Ehrlichia spp. and Anaplasma phagocytophilum in Wisconsin is important information as a baseline for future comparisons. Reported human cases between 2009 and 2015 were identified using the Wisconsin Electronic Disease Surveillance System (WEDSS) and mapped by county of residence. Vector surveillance was established using ticks collected from animals by partners, including veterinary medical clinics, domestic animal shelters, and wildlife rehabilitation centers from 40 Wisconsin counties...
January 5, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28036138/early-diagnosis-of-ehrlichia-ewingii-infection-in-a-lung-transplant-recipient-by-peripheral-blood-smear
#8
Hariharan Regunath, Christian Rojas-Moreno, Juan P Olano, Richard D Hammer, William Salzer
Ehrlichiosis in lung transplant (LT) recipients is associated with severe outcomes. Ehrlichia ewingii is a less frequent cause of symptomatic ehrlichiosis, characterized by cytoplasmic inclusions (morulae) within circulating neutrophils. We report a case of E. ewingii infection in a LT recipient diagnosed promptly by blood smear exam and confirmed with molecular studies. This article is protected by copyright. All rights reserved.
December 30, 2016: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28031351/evaluation-of-gulf-coast-ticks-acari-ixodidae-for-ehrlichia-and-anaplasma-species
#9
Michelle E J Allerdice, Joy A Hecht, Sandor E Karpathy, Christopher D Paddock
Amblyomma maculatum Koch (the Gulf Coast tick) is an aggressive, human-biting ixodid tick distributed throughout much of the southeastern United States and is the primary vector for Rickettsia parkeri, an emerging human pathogen. Amblyomma maculatum has diverse host preferences that include white-tailed deer, a known reservoir for Ehrlichia and Anaplasma species, including the human pathogens E. ewingii and E. chaffeensis To examine more closely the potential role of A. maculatum in the maintenance of various pathogenic Ehrlichia and Anaplasma species, we screened DNA samples from 493 questing adult A...
December 28, 2016: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28011735/occurrence-of-amblyomma-americanum-acari-ixodidae-and-human-infection-with-ehrlichia-chaffeensis-in-wisconsin-2008-2015
#10
Megan Christenson, Xia Lee, Scott Larson, Diep Hoang Johnson, Julia Jensen, Megan Meller, Susan Paskewitz
Because of the increasing incidence of human ehrlichiosis in Wisconsin, we assessed reports of human infections by Ehrlichia chaffeensis and the distribution of its vector, the lone star tick (Amblyomma americanum (L.)). From 2008 through 2015, 158 probable and confirmed human cases of E. chaffeensis infections were reported to the Wisconsin Department of Health Services. Five cases without travel history outside of Wisconsin were confirmed as E. chaffeensis by polymerase chain reaction. Surveillance for the vector occurred from 2008 through 2015 and was based on active and passive methods, including examination of white-tailed deer, collections from live-trapped small mammals, submissions of ticks removed from wild and domestic animals through the Wisconsin Surveillance of Animals for Ticks (SWAT) program, digital or physical submissions by the public to the University of Wisconsin Insect Diagnostic or Medical Entomology laboratories, and active tick dragging...
December 23, 2016: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28011185/novel-rickettsia-and-emergent-tick-borne-pathogens-a-molecular-survey-of-ticks-and-tick-borne-pathogens-in-shimba-hills-national-reserve-kenya
#11
Micky M Mwamuye, Edward Kariuki, David Omondi, James Kabii, David Odongo, Daniel Masiga, Jandouwe Villinger
Ticks are important vectors of emerging and re-emerging zoonoses, the majority of which originate from wildlife. In recent times, this has become a global public health concern that necessitates surveillance of both known and unknown tick-borne pathogens likely to be future disease threats, as well as their tick vectors. We carried out a survey of the diversity of ticks and tick-borne pathogens in Kenya's Shimba Hills National Reserve (SHNR), an area with intensified human-livestock-wildlife interactions, where we collected 4297 questing ticks (209 adult ticks, 586 nymphs and 3502 larvae)...
September 5, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27978844/detection-of-mycoplasma-haemocanis-mycoplasma-haematoparvum-mycoplasma-suis-and-other-vector-borne-pathogens-in-dogs-from-c%C3%A3-rdoba-and-santa-f%C3%A3-argentina
#12
Patricia E Mascarelli, Gustavo P Tartara, Norma B Pereyra, Ricardo G Maggi
BACKGROUND: In Argentina, only very few reports are available for canine tick-borne diseases where most are related to parasitic diseases. The objective of this survey was to investigate the prevalence of tick-borne pathogens in 70 dogs from Santa Fé and Córdoba, Argentina. METHODS: Microscopic blood smear examination as well as polymerase chain reaction (PCR) amplification using species-specific markers of Anaplasma, Babesia, Bartonella, Borrelia, Ehrlichia, Francisella, Mycoplasma (hemotropic group) and Rickettsia, followed by DNA sequencing were used to establish the prevalence of each infecting pathogen...
December 15, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27966221/determination-of-the-microbial-community-features-of-haemaphysalis-flava-in-different-developmental-stages-by-high-throughput-sequencing
#13
Deyong Duan, Tianyin Cheng
In this study, we analyzed the in vivo microbial community structure and diversity differences of Haemaphysalis flava (H. flava) in four developmental stages (egg, larva, nymph, and adult) to determine which bacterial genera could be propagated through transovarial transmission and transmitted by transstadial transmission during different developmental stages of H. flava. Paired-end sequencing of the V3 region of the 16S ribosomal DNA (16S rDNA) of H. flava in four developmental stage samples was conducted using the Illumina MiSeq sequencing platform...
December 14, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27965644/molecular-detection-and-characterization-of-zoonotic-and-veterinary-pathogens-in-ticks-from-northeastern-china
#14
Feng Wei, Mingxin Song, Huanhuan Liu, Bo Wang, Shuchao Wang, Zedong Wang, Hongyu Ma, Zhongyu Li, Zheng Zeng, Jun Qian, Quan Liu
Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27934910/extensive-genetic-diversity-of-rickettsiales-bacteria-in-multiple-mosquito-species
#15
Wen-Ping Guo, Jun-Hua Tian, Xian-Dan Lin, Xue-Bing Ni, Xiao-Ping Chen, Yong Liao, Si-Yuan Yang, J Stephen Dumler, Edward C Holmes, Yong-Zhen Zhang
Rickettsiales are important zoonotic pathogens, causing severe disease in humans globally. Although mosquitoes are an important vector for diverse pathogens, with the exception of members of the genus Wolbachia little is known about their role in the transmission of Rickettsiales. Herein, Rickettsiales were identified by PCR in five species of mosquitoes (Anopheles sinensis, Armigeres subalbatus, Aedes albopictus, Culex quinquefasciatus and Cu. tritaeniorhynchus) collected from three Chinese provinces during 2014-2015...
December 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27913878/piroplasms-in-brown-hyaenas-parahyaena-brunnea-and-spotted-hyaenas-crocuta-crocuta-in-namibia-and-south-africa-are-closely-related-to-babesia-lengau
#16
Richard E J Burroughs, Barend L Penzhorn, Ingrid Wiesel, Nancy Barker, Ilse Vorster, Marinda C Oosthuizen
The objective of our study was identification and molecular characterization of piroplasms and rickettsias occurring in brown (Parahyaena brunnea) and spotted hyaenas (Crocuta crocuta) from various localities in Namibia and South Africa. Whole blood (n = 59) and skin (n = 3) specimens from brown (n = 15) and spotted hyaenas (n = 47) were screened for the presence of Babesia, Theileria, Ehrlichia and Anaplasma species using the reverse line blot (RLB) hybridization technique. PCR products of 52/62 (83...
February 2017: Parasitology Research
https://www.readbyqxmd.com/read/27908772/anaplasma-platys-in-dogs-from-uruguay
#17
Luis Carvalho, Maria Teresa Armua-Fernandez, Nicolás Sosa, María Laura Félix, José Manuel Venzal
Anaplasmataceae family members include vector-borne bacteria of veterinary importance that may also affect humans. Ehrlichia canis and Anaplasma platys are the main members of this family detected in dogs worldwide. In Uruguay there are not many published studies on tick-borne pathogens affecting dogs, the only haemoparasite molecularly confirmed in dogs, is the piroplasm Rangelia vitalii. The aim of the present work was to detect the presence of A. platys and E. canis in dogs and dogs-associated ticks of two localities in Northwestern Uruguay...
November 17, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27902186/multigenetic-characterization-of-candidatus-xenohaliotis-californiensis
#18
Francesco Cicala, James D Moore, Jorge Cáceres-Martínez, Miguel A Del Río-Portilla, Mónica Hernández-Rodríguez, Rebeca Vásquez-Yeomans, Axayácatl Rocha-Olivares
'Candidatus Xenohaliotis californiensis' (or Ca.Xc) is the aetiological agent of withering syndrome, a chronic wasting disease affecting most if not all North American species of abalone, and has been described as a Rickettsiales-like prokaryote. Genetic data regarding this species are limited to the 16S rRNA gene. The inability to grow it axenically has hindered its genetic and genomic characterization and, in consequence, a thorough analysis of its systematics. Here, we amplified and sequenced five genes (16S rRNA, 23S rRNA, ftsZ, virD4 and virB11) of Ca...
January 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27894847/pcr-screening-of-tick-borne-agents-in-sensitive-conservation-areas-southeast-portugal
#19
Maria Margarida Santos-Silva, Pedro Melo, Nuno Santos, Sandra Antunes, Luís Raposo Duarte, Joana Ferrolho, Natacha Milhano, Patrícia Tavares Santos, Ana Domingos, Ana Sofia Santos
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs)...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27890889/first-molecular-detection-of-tick-borne-pathogens-in-dogs-from-jiangxi-china
#20
Weiqing Zheng, Mingming Liu, Paul Franck Adjou Moumouni, Xiaoqing Liu, Artemis Efstratiou, Zhanbin Liu, Yangqing Liu, Huiying Tao, Huanping Guo, Guanbo Wang, Yang Gao, Zifen Li, Aaron Edmund Ringo, Charoonluk Jirapattharasate, Haiying Chen, Xuenan Xuan
In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp...
November 26, 2016: Journal of Veterinary Medical Science
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