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Ehrlichia

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https://www.readbyqxmd.com/read/28807290/exposure-to-vector-borne-pathogens-in-privately-owned-dogs-living-in-different-socioeconomic-settings-in-brazil
#1
Luciana Aguiar Figueredo, Kamila Gaudêncio da Silva Sales, Katrin Deuster, Matthias Pollmeier, Domenico Otranto, Filipe Dantas-Torres
This survey was conducted in four Brazilian cities, from three federative units (Pernambuco, Minas Gerais, and Federal District), representing different socioeconomic settings, to determine the presence of antibodies to or antigens and DNA of selected pathogens in privately owned dogs. From January to April 2015, blood and serum samples were collected and assayed using different tests. In particular, antibodies to Anaplasma spp., Borrelia burgdorferi sensu lato, and Ehrlichia spp., and antigens of Dirofilaria immitis were detected using a rapid enzyme-linked immunosorbent assay, whereas antibodies to Babesia spp...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28776227/seroprevalence-and-risk-factors-associated-with-ehrlichia-canis-anaplasma-spp-borrelia-burgdorferi-sensu-lato-and-d-immitis-in-hunting-dogs-from-southern-italy
#2
Diego Piantedosi, Benedetto Neola, Nicola D'Alessio, Francesca Di Prisco, Mario Santoro, Laura Pacifico, Giovanni Sgroi, Luigi Auletta, Jesse Buch, Ramaswamy Chandrashekar, Edward B Breitschwerdt, Vincenzo Veneziano
Canine vector-borne diseases (CVBDs) are caused by a range of pathogens transmitted to dogs by arthropods. The present study investigates Ehrlichia canis, Anaplasma spp., Borrelia burgdorferi sensu lato, and Dirofilaria immitis seroprevalences in hunting dogs from southern Italy. Dogs (no. 1335) were tested using a commercial in-clinic enzyme-linked immunosorbent assay kit. Odds ratios (ORs) were calculated by logistic regression analysis to identify risk factors. Overall, 138/1335 dogs (10.3%) were seroreactive to at least one CVBD pathogen...
August 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28751428/cerebral-haemorrhage-as-a-clinical-manifestation-of-human-ehrlichiosis
#3
Catalina Garc Ía-Baena, MarÍa Fernanda Cárdenas, Juan Fernando Ramón
A 16-year-old young man presented to the emergency room with new-onset generalised tonic-clonic seizures. Examination showed a Glasgow score of 13 and predominantly crural left hemiparesis. Imaging demonstrated a right frontoparietal haemorrhage of non-vascular origin with perilesional oedema. Surgical drainage was carried out, but rebleeding occurred within 24 hours following surgery, and again 1 week after discharge. On reinterrogation and examination, Ehrlichia canis infection was suspected and empirical management with doxycycline was begun...
July 27, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28750866/two-novel-ehrlichia-strains-detected-in-amblyomma-tigrinum-ticks-associated-to-dogs-in-peri-urban-areas-of-argentina
#4
Gabriel L Cicuttin, M Nazarena De Salvo, Santiago Nava
The aim of this work was to describe two novel strains of Ehrlichia associated to Amblyomma tigrinum from Argentina. Molecular detection of agents belonging to the family Anaplasmataceae was performed targeting three different loci: 16S rRNA gene, dsb gene and a fragment of groESL heat shock operon. The results have shown that two different strains of Ehrlichia sp. associated to A. tigrinum are circulating in peri-urban areas of Argentina. The Ehrlichia strain detected in ticks from San Luis Province, named as Ehrlichia sp...
August 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28750787/high-dose-filgrastim-treatment-of-nonregenerative-pancytopenia-associated-with-chronic-canine-ehrlichiosis
#5
Mauricio Palacios, Rafael Arteaga, Gustavo Calvo
OBJECTIVE: To report the management and outcome of a dog with canine monocytic ehrlichiosis and nonregenerative pancytopenia, with high doses of filgrastim. CASE DESCRIPTION: An 8-year-old male, mixed-breed dog, weighing 5.6kg, presented with a 1-month history of hyporexia, adynamia, and a weight loss of approximately 1kg. The general condition of the dog was observed to be poor as follows: lethargy, tachycardia, marked pallor of the mucous membranes, petechiae on the abdomen, hepatosplenomegaly, and cervical lymphadenopathy...
March 2017: Topics in Companion Animal Medicine
https://www.readbyqxmd.com/read/28749278/survey-of-anaplasma-phagocytophilum-antibodies-in-captive-przewalski-s-horses-equus-ferus-przewalskii
#6
Richard R Sim, Luis R Padilla, Priscilla H Joyner, Paul Anikis, Copper Aitken-Palmer
Anaplasma phagocytophilum (formerly Ehrlichia equi ) is a tickborne pathogen of domestic horses and the causative agent of equine granulocytic anaplasmosis. After the occurrence of clinical anaplasmosis in a Przewalski's horse ( Equus ferus przewalskii) housed at the Smithsonian Conservation Biology Institute in 2008, opportunistic serosurveillance of the herd was initiated. From 2008 to 2014, 57 serum samples were collected from 27 individuals (10 males; 17 females). Using indirect immunofluorescent antibody assays for anti- Anaplasma phagocytophilum antibodies, it was determined that prevalence was 53%...
June 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28739301/a-new-pcr-assay-for-the-detection-and-differentiation-of-babesia-canis-and-babesia-vogeli
#7
Giada Annoscia, Maria Stefania Latrofa, Cinzia Cantacessi, Emanuela Olivieri, Maria Teresa Manfredi, Filipe Dantas-Torres, Domenico Otranto
Babesia spp. are globally distributed tick-borne protozoan parasites that infect the red blood cells of a wide range of vertebrate hosts, including humans. Diagnosis of babesiosis is often impeded by the transient presence of the parasites in peripheral blood, as well as by their pleomorphic nature. Given the reports of an expanding and, in some cases, sympatric geographical distribution of Babesia canis and Babesia vogeli in dogs and associated vectors, in Europe, the development of time-efficient and cost-effective diagnostic tools to detect and differentiate these two species is warranted...
July 8, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28705746/first-report-of-ehrlichia-infection-in-goats-china
#8
Hui Zhang, Zhenyu Chang, Khalid Mehmood, Yajing Wang, Mujeeb Ur Rehman, Fazul Nabi, Ahamad Jawad Sabir, Xueting Liu, Xiaoxing Wu, Xinxin Tian, Donghai Zhou
Ehrlichiosis is an emerging infectious disease of domestic animals which is transmitted by ticks. This disease has been reported earlier in most parts of China in dogs, cattle and humans, but there is no published data regarding this disease in goats. The present study provided the evidence of Ehrlichia infection in goats in Wuhan, China on the basis of clinical signs, gross lesions, serum-biochemical, histopathological and PCR. Twenty four goats were presented to the veterinary hospital of Huazhong Agricultural University during July, 2016...
July 11, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28700062/seropositivity-for-rickettsia-spp-and-ehrlichia-spp-in-the-human-population-of-mato-grosso-central-western-brazil
#9
Maria Cristina Fuzari Bezerra, Andréia Lima Tomé Melo, Isis Indaiara Gonçalves Granjeiro Taques, Daniel Moura de Aguiar, Richard Campos Pacheco, Renata Dezengrini Slhessarenko
INTRODUCTION: The epidemiology of Rickettsia and Ehrlichia species infection is underestimated in Mato Grosso State. METHODS: Serum samples obtained during a Dengue outbreak in 2011-2012 were tested via indirect immunofluorescence and/or ELISA. RESULTS: Samples from 19/506 (3.8%) patients presented antibodies for at least one of three Rickettsia species; 2/506 (0.4%) samples reacted against Ehrlichia canis. Most afflicted patients are residents of cities from the south-central region of the state, where these diseases have been reported in animals...
May 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28699575/proposal-to-reclassify-ehrlichia-muris-as-ehrlichia-muris-subsp-muris-subsp-nov-and-description-of-ehrlichia-muris-subsp-eauclairensis-subsp-nov-a-newly-recognized-tick-borne-pathogen-of-humans
#10
Bobbi S Pritt, Michelle E J Allerdice, Lynne M Sloan, Christopher D Paddock, Ulrike G Munderloh, Yasuko Rikihisa, Tomoko Tajima, Susan M Paskewitz, David F Neitzel, Diep K Hoang Johnson, Elizabeth Schiffman, Jeffrey P Davis, Cynthia S Goldsmith, Curtis M Nelson, Sandor E Karpathy
We have previously described a novel taxon of the genus Ehrlichia (type strain WisconsinT), closely related to Ehrlichia muris, that causes human ehrlichiosis among patients with exposures to ticks in the upper midwestern USA. DNA from this bacterium was also detected in Ixodes scapularis and Peromyscus leucopus collected in Minnesota and Wisconsin. To determine the relationship between the E. muris-like agent (EMLA) and other species of the genus Ehrlichia phenotypic, genotypic and epidemiologic comparisons were undertaken, including sequence analysis of eight gene loci (3906 nucleotides) for 39 EMLA DNA samples and the type strain of E...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28690147/novel-anaplasma-and-ehrlichia-organisms-infecting-the-wildlife-of-two-regions-of-the-brazilian-amazon
#11
Herbert S Soares, Arlei Marcili, Amália R M Barbieri, Antonio H H Minervino, Antonio F Malheiros, Solange M Gennari, Marcelo B Labruna
During 2009-2012, wild animals were sampled in the Amazon biome of Brazil. Animal tissues and blood were tested by polymerase chain reaction (PCR) assays targeting DNA of the bacterial family Anaplasmataceae (genera Anaplasma, Ehrlichia, Wolbachia) and the genus Borrelia. Overall, 181 wild animals comprising 36 different species (2 reptiles, 5 birds, and 29 mammals) were sampled. All birds and reptiles were negative by all PCR assays, as well as all mammals for the Borrelia PCR assay. Anaplasmataceae agents were searched by PCR assays targeting two different genes, the ribosomal 16S rRNA gene and the protein-coding dsb gene...
July 6, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28682765/a-pcr-survey-of-vector-borne-pathogens-in-different-dog-populations-from-turkey
#12
Huanping Guo, Ferda Sevinc, Onur Ceylan, Mutlu Sevinc, Ege Ince, Yang Gao, Paul Franck Adjou Moumouni, Mingming Liu, Artemis Efstratiou, Guanbo Wang, Shinuo Cao, Mo Zhou, Charoonluk Jirapattharasate, Aaron Edmond Ringo, Weiqing Zheng, Xuenan Xuan
In the present study, a total of 192 blood samples were collected from pet dogs, kennel dogs and shepherd dogs in Konya district, Turkey, and tested by specific PCR for the presence of vector-borne pathogens. Several pathogens were identified, most of which can cause substantial morbidity in dogs. PCR results revealed that 54 (28.1%) dogs were infected with one or more pathogens. Positive results were obtained for Babesia spp. in 4 dogs (2.1%), Hepatozoon spp. in 8 dogs (4.2%) and Mycoplasma spp. in 46 dogs (24%)...
September 26, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28673455/neorickettsia-risticii-rickettsia-sp-and-bartonella-sp-in-tadarida-brasiliensis-bats-from-buenos-aires-argentina
#13
Gabriel L Cicuttin, María N De Salvo, Isabel La Rosa, Federico E Gury Dohmen
Bats are potential reservoirs of many vector-borne bacterial pathogens. The aim of the present study was to detect species of Anaplasma, Ehrlichia, Neorickettsia, Rickettsia, Borrelia and Bartonella in Brazilian free-tailed bats (Tadarida brasiliensis, Molossidae) from Buenos Aires city, Argentina. Between 2012 and 2013, 61 T. brasiliensis from urban areas of Buenos Aires city were studied. The samples were molecularly screened by PCR and sequencing. Five bats (8.2%) were positive to Neorickettsia risticii, one (1...
June 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28669382/new-wide-dynamic-range-assays-for-quantification-of-anti-leishmania-igg2-and-iga-antibodies-in-canine-serum
#14
Ana Cantos-Barreda, Damián Escribano, Luis J Bernal, Luis Pardo-Marín, José J Cerón, Silvia Martínez-Subiela
The aims of this study were (1) to develop and validate time resolved-immunofluorometric assays for the detection of anti-Leishmania IgG2 and IgA antibodies in canine serum and (2) to evaluate the ability of these assays to quantify different amounts of anti-Leishmania antibodies in Leishmania-seronegative and seropositive dogs, determined by a commercial ELISA assay, and between different clinical stages according to LeishVet guidelines. The analytical validation showed that the assays had a good precision with intra- and inter-assay coefficients of variation lower than 10%...
July 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28658415/ehrlichia-canis-and-rickettsia-spp-in-dogs-from-urban-areas-in-paraiba-state-northeastern-brazil
#15
Tereza Emmanuelle de Farias Rotondano, Felipe da Silva Krawczak, Werona de Oliveira Barbosa, Jonas Moraes-Filho, Fernanda Nieri Bastos, Marcelo Bahia Labruna, Sérgio Santos de Azevedo, Marcia Almeida de Melo, Alzira Maria Paiva de Almeida
The aims of our study was to identify Ehrlichia canis and antibodies against Rickettsia spp. belonging to the spotted fever group (SFG) in dogs sampled from Paraiba state, northeastern Brazil. Blood and serum samples collected by convenience from dogs in urban areas of five municipalities were analyzed by real-time PCR for the detection of E. canis DNA and by immunofluorescence assay test (IFAT) for the identification of antibodies against Rickettsia rickettsii, R. felis, R. parkeri, R. amblyommii and R. rhipicephali antigens...
April 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28657860/prevalence-of-babesia-spp-ehrlichia-spp-and-tick-infestations-in-oklahoma-black-bears-ursus-americanus
#16
Delaina Skinner, Jessica R Mitcham, Lindsay A Starkey, Bruce H Noden, W Sue Fairbanks, Susan E Little
American black bears (Ursus americanus) are commonly infested with ticks throughout their range, but there are few surveys for tick-borne disease agents in bears. To characterize tick infestations and determine the prevalence of current infection with Babesia spp. and past or current infection with Ehrlichia spp. in newly re-established populations of black bears in east central and southeastern Oklahoma, we identified adult (n=1,048) and immature (n=107) ticks recovered from bears (n=62). We evaluated serum and whole blood samples from a subset (n=49) for antibodies reactive to, and characteristic DNA fragments of Ehrlichia spp...
June 28, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28650718/subversion-of-rab5-regulated-autophagy-by-the-intracellular-pathogen-ehrlichia-chaffeensis
#17
Yasuko Rikihisa
Intracellular pathogens often exploit RAB functions to establish a safe haven in which to survive and proliferate. Ehrlichia chaffeensis, an obligatory intracellular bacterium, resides in specialized membrane-bound inclusions that have early endosome-like characteristics, e.g., resident RAB5 GTPase and RAB5 effectors, including VPS34 (the catalytic subunit of class III phosphatidylinositol 3-kinase), but the inclusions lack late endosomal or lysosomal markers. Within inclusions, Ehrlichia obtains host-derived nutrients by inducing RAB5-regulated autophagy using Ehrlichia translocated factor-1 deployed by its type IV secretion system...
June 26, 2017: Small GTPases
https://www.readbyqxmd.com/read/28645313/molecular-investigation-and-phylogeny-of-anaplasmataceae-species-infecting-domestic-animals-and-ticks-in-corsica-france
#18
Mustapha Dahmani, Bernard Davoust, Djamel Tahir, Didier Raoult, Florence Fenollar, Oleg Mediannikov
BACKGROUNDS: Corsica is a French island situated in the Mediterranean Sea. The island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. The family Anaplasmataceae is a member of the order Rickettsiales; it includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia. Anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera Anaplasma and Ehrlichia...
June 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28638803/peptide-nucleic-acid-knockdown-and-intra-host-cell-complementation-of-ehrlichia-type-iv-secretion-system-effector
#19
Pratibha Sharma, Omid Teymournejad, Yasuko Rikihisa
Survival of Ehrlichia chaffeensis depends on obligatory intracellular infection. One of the barriers to E. chaffeensis research progress has been the inability, using conventional techniques, to generate knock-out mutants for genes essential for intracellular infection. This study examined the use of Peptide Nucleic Acids (PNAs) technology to interrupt type IV secretion system (T4SS) effector protein expression in E. chaffeensis followed by intracellular complementation of the effector to determine its requirement for infection...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28633666/molecular-detection-of-vector-borne-pathogens-in-dogs-and-cats-from-qatar
#20
Ana Margarida Alho, Clara Lima, Maria Stefania Latrofa, Vito Colella, Silvia Ravagnan, Gioia Capelli, Luís Madeira de Carvalho, Luís Cardoso, Domenico Otranto
BACKGROUND: Vector-borne diseases (VBDs) have been increasingly reported in dogs and cats worldwide. However, no data are currently available regarding canine and feline VBDs in Qatar and limited information is available from other Persian Gulf countries. METHODS: Blood samples from 98 client-owned animals (i.e. 64 dogs and 34 cats) living in Doha (Qatar) were collected and the presence of genomic DNA of Anaplasma spp., Babesia spp., Dirofilaria spp., Ehrlichia spp...
June 20, 2017: Parasites & Vectors
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