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Forensic genetics

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https://www.readbyqxmd.com/read/28938153/massively-parallel-sequencing-of-32-forensic-markers-using-the-precision-id-globalfiler%C3%A2-ngs-str-panel-and-the-ion-pgm%C3%A2-system
#1
Zheng Wang, Di Zhou, Hui Wang, Zhenjun Jia, Jing Liu, Xiaoqin Qian, Chengtao Li, Yiping Hou
Massively parallel sequencing (MPS) technologies have proved capable of sequencing the majority of the key forensic STR markers. By MPS, not only the repeat-length size but also sequence variations could be detected. Recently, Thermo Fisher Scientific has designed an advanced MPS 32-plex panel, named the Precision ID GlobalFiler™ NGS STR Panel, where the primer set has been designed specifically for the purpose of MPS technologies and the data analysis are supported by a new version HID STR Genotyper Plugin (V4...
September 8, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28938152/comparison-of-manual-and-automated-ampliseq%C3%A2-workflows-in-the-typing-of-a-somali-population-with-the-precision-id-identity-panel
#2
Suzanne van der Heijden, Susanne Juel de Oliveira, Marie-Louise Kampmann, Claus Børsting, Niels Morling
The Precision ID Identity Panel was used to type 109 Somali individuals in order to obtain allele frequencies for the Somali population. These frequencies were used to establish a Somali HID-SNP database, which will be used for the biostatistic calculations in family and immigration cases. Genotypes obtained with the Precision ID Identity Panel were found to be almost in complete concordance with genotypes obtained with the SNPforID PCR-SBE-CE assay. In seven SNP loci, silent alleles were identified, of which most were previously described in the literature...
September 14, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28938133/genetic-polymorphisms-for-19-x-str-loci-of-sichuan-han-ethnicity-and-its-comparison-with-chinese-populations
#3
Guanglin He, Ye Li, Xing Zou, Mengge Wang, Pengyu Chen, Miao Liao, Jin Wu
X-chromosomal short tandem repeats (X-STRs) can be serviced as a complementary tool in forensic deficiency cases and other complicated kinship identification. To investigate the genetic variation of the western Chinese Han population, genetic polymorphisms of 19 X-STR loci (DXS8378, DXS7423, DXS10148, DXS10159, DXS10134, DXS7424, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103, DXS10101, HPRTB, DXS6809, DXS10075, DXS10074 and DXS10135) included in the AGCU X19 PCR amplification kit were obtained from 201 Chinese Han individuals (108 females and 93 males) residing in Sichuan province, western China...
September 15, 2017: Legal Medicine
https://www.readbyqxmd.com/read/28936824/discrimination-of-human-and-nonhuman-blood-using-raman-spectroscopy-with-self-reference-algorithm
#4
Haiyi Bian, Peng Wang, Jun Wang, Huancai Yin, Yubing Tian, Pengli Bai, Xiaodong Wu, Ning Wang, Yuguo Tang, Jing Gao
We report a self-reference algorithm to discriminate human and nonhuman blood by calculating the ratios of identification Raman peaks to reference Raman peaks and choosing appropriate threshold values. The influence of using different reference peaks and identification peaks was analyzed in detail. The Raman peak at 1003  cm-1 was proved to be a stable reference peak to avoid the influencing factors, such as the incident laser intensity and the amount of sample. The Raman peak at 1341  cm-1 was found to be an efficient identification peak, which indicates that the difference between human and nonhuman blood results from the C-H bend in tryptophan...
September 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28934201/forensic-genetics-and-genomics-much-more-than-just-a-human-affair
#5
REVIEW
Miguel Arenas, Filipe Pereira, Manuela Oliveira, Nadia Pinto, Alexandra M Lopes, Veronica Gomes, Angel Carracedo, Antonio Amorim
While traditional forensic genetics has been oriented towards using human DNA in criminal investigation and civil court cases, it currently presents a much wider application range, including not only legal situations sensu stricto but also and, increasingly often, to preemptively avoid judicial processes. Despite some difficulties, current forensic genetics is progressively incorporating the analysis of nonhuman genetic material to a greater extent. The analysis of this material-including other animal species, plants, or microorganisms-is now broadly used, providing ancillary evidence in criminalistics in cases such as animal attacks, trafficking of species, bioterrorism and biocrimes, and identification of fraudulent food composition, among many others...
September 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28934125/optimized-mtdna-control-region-primer-extension-capture-analysis-for-forensically-relevant-samples-and-highly-compromised-mtdna-of-different-age-and-origin
#6
Mayra Eduardoff, Catarina Xavier, Christina Strobl, Andrea Casas-Vargas, Walther Parson
The analysis of mitochondrial DNA (mtDNA) has proven useful in forensic genetics and ancient DNA (aDNA) studies, where specimens are often highly compromised and DNA quality and quantity are low. In forensic genetics, the mtDNA control region (CR) is commonly sequenced using established Sanger-type Sequencing (STS) protocols involving fragment sizes down to approximately 150 base pairs (bp). Recent developments include Massively Parallel Sequencing (MPS) of (multiplex) PCR-generated libraries using the same amplicon sizes...
September 21, 2017: Genes
https://www.readbyqxmd.com/read/28915619/autosomal-indel-polymorphisms-for-population-genetic-structure-and-differentiation-analysis-of-chinese-kazak-ethnic-group
#7
Tingting Kong, Yahao Chen, Yuxin Guo, Yuanyuan Wei, Xiaoye Jin, Tong Xie, Yuling Mu, Qian Dong, Shaoqing Wen, Boyan Zhou, Li Zhang, Chunmei Shen, Bofeng Zhu
In the present study, we assessed the genetic diversities of the Chinese Kazak ethnic group on the basis of 30 well-chosen autosomal insertion and deletion loci and explored the genetic relationships between Kazak and 23 reference groups. We detected the level of the expected heterozygosity ranging from 0.3605 at HLD39 locus to 0.5000 at HLD136 locus and the observed heterozygosity ranging from 0.3548 at HLD39 locus to 0.5283 at HLD136 locus. The combined power of discrimination and the combined power of exclusion for all 30 loci in the studied Kazak group were 0...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28903432/genetic-variation-and-forensic-characteristic-analysis-of-25-strs-of-a-novel-fluorescence-co-amplification-system-in-chinese-southern-shaanxi-han-population
#8
Yao-Shun Liu, Jian-Gang Chen, Ting Mei, Yu-Xin Guo, Hao-Tian Meng, Jian-Fei Li, Yuan-Yuan Wei, Xiao-Ye Jin, Bo-Feng Zhu, Li-Ping Zhang
We analyzed the genetic polymorphisms of 15 autosomal and 10 Y-chromosomal STR loci in 214 individuals of Han population from Southern Shaanxi of China and studied the genetic relationships between Southern Shaanxi Han and other populations. We observed a total of 150 alleles at 15 autosomal STR loci with the corresponding allelic frequencies ranging from 0.0023 to 0.5210, and the combined power of discrimination and exclusion for the 15 autosomal STR loci were 0.99999999999999998866 and 0.999998491, respectively...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28900075/-circulating-micrornas-in-the-identification-of-biological-fluids-a-new-approach-to-standardization-of-expression-based-diagnostics
#9
A S Bavykin
Molecular profiling of normal tissues is a regular and necessary step when developing systems for expression analyses in biological samples, including diagnostic panels for various diseases and conditions. Yet there are still no rigorous criteria to allow precise typing of normal tissues. A main problem is that the methods employed in diagnostic expression testing are difficult to standardize. While various technologies, instruments, and reagents are available, universal protocols of handling biological material are lacking, thus impairing the reproducibility of data from independent studies...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28889862/genetic-dna-profile-in-urine-and-hair-follicles-from-patients-who-have-undergone-allogeneic-hematopoietic-stem-cell-transplantation
#10
Ana Santurtún, José A Riancho, Maite Santurtún, Carlos Richard, M Mercedes Colorado, Mayte García Unzueta, María T Zarrabeitia
Biological samples from patients who have undergone allogeneic hematopoietic stem cell transplantation (HSCT) constitute a challenge for individual identification. In this study we analyzed the genetic profiles (by the amplification of 15 autosomic STRs) of HSCT patients found in different types of samples (blood, hair and urine) that may be the source of DNA in civil or criminal forensic cases. Our results show that while in hair follicles the donor component was not detected in any patient, thus being a reliable source of biological material for forensic identification, mixed chimerism was detected in urine samples from all patient, and no correlation was found between the time elapsed from the transplant and the percentage of chimerism...
September 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28889247/guidelines-for-autopsy-investigation-of-sudden-cardiac-death-2017-update-from-the-association-for-european-cardiovascular-pathology
#11
Cristina Basso, Beatriz Aguilera, Jytte Banner, Stephan Cohle, Giulia d'Amati, Rosa Henriques de Gouveia, Cira di Gioia, Aurelie Fabre, Patrick J Gallagher, Ornella Leone, Joaquin Lucena, Lubov Mitrofanova, Pilar Molina, Sarah Parsons, Stefania Rizzo, Mary N Sheppard, Maria Paz Suárez Mier, S Kim Suvarna, Gaetano Thiene, Allard van der Wal, Aryan Vink, Katarzyna Michaud
Although sudden cardiac death (SCD) is one of the most important modes of death in Western countries, pathologists and public health physicians have not given this problem the attention it deserves. New methods of preventing potentially fatal arrhythmias have been developed and the accurate diagnosis of the causes of SCD is now of particular importance. Pathologists are responsible for determining the precise cause and mechanism of sudden death but there is still considerable variation in the way in which they approach this increasingly complex task...
September 9, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28887705/population-data-and-mutation-rates-of-20-autosomal-str-loci-in-a-chinese-han-population-from-yunnan-province-southwest-china
#12
Xiufeng Zhang, Linlin Liu, Runfang Xie, Guiyi Wang, Yuan Shi, Tao Gu, Liping Hu, Shengjie Nie
The genetic polymorphisms of 20 autosomal short tandem repeat (STR) loci included in the PowerPlex® 21 kit were evaluated from 2068 unrelated, healthy individuals from the Chinese Han population of Yunnan Province in southwest China. All of the loci reached Hardy-Weinberg equilibrium. These loci were examined to determine allele frequencies and forensic statistical parameters. The genetic relationships among the Yunnan Han and other Chinese populations were also estimated. The combined discrimination power and probability of excluding paternity of the 20 STR loci were 0...
September 8, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28887423/forensic-human-identification-using-skin-microbiomes
#13
Sarah E Schmedes, August E Woerner, Bruce Budowle
The human microbiome contributes significantly to the genetic content of the human body. Genetic and environmental factors help shape the microbiome, and as such, the microbiome can be unique to an individual. Previous studies have demonstrated the potential to use microbiome profiling for forensic applications, however a method has yet to identify stable features of skin microbiomes that produce high classification accuracies for samples collected over reasonably long time intervals. A novel approach is described to classify skin microbiomes to their donors by comparing two features types, Propionibacterium acnes pangenome presence/absence features and nucleotide diversities of stable clade-specific markers...
September 8, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28886207/the-biological-effects-of-kambo-is-there-a-relationship-between-its-administration-and-sudden-death
#14
Isabella Aquila, Santo Gratteri, Matteo A Sacco, Vittorio Fineschi, Simona Magi, Pasqualina Castaldo, Graziella Viscomi, Salvatore Amoroso, Pietrantonio Ricci
Kambo is a substance obtained from the skin secretions of a frog, Phyllomedusa bicolor, popular in the Amazon region, which is administered via the transdermal route. We report a case of 42-year-old man found dead in his house. Near the corpse, a plastic box labeled as "Kambo sticks" was found. The man was a chronic consumer of Kambo and no previous pathology or genetic disease emerged in clinical history from the declaration of his general practitioner. Autopsy investigations and toxicological analysis were performed...
September 8, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28874020/is-pmi-the-hypothesis-or-the-null-hypothesis
#15
Aaron M Tarone, Michelle R Sanford
Over the past several decades, there have been several strident exchanges regarding whether forensic entomologists estimate the postmortem interval (PMI), minimum PMI, or something else. During that time, there has been a proliferation of terminology reflecting this concern regarding "what we do." This has been a frustrating conversation for some in the community because much of this debate appears to be centered on what assumptions are acknowledged directly and which are embedded within a list of assumptions (or ignored altogether) in the literature and in case reports...
September 1, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28871330/forensic-features-and-phylogenetic-analyses-of-sichuan-han-population-via-23-autosomal-str-loci-included-in-the-huaxia-platinum-system
#16
Guanglin He, Mengge Wang, Jing Liu, Yiping Hou, Zheng Wang
Short tandem repeats (STRs) are DNA regions with a variable number of repeated units (2-6 bp) and attractive to genetic application like forensic, population genetics, and anthropological studies. The Huaxia Platinum System allows the multiplex amplification of 23 autosomal STR loci and two gender determination loci. In the present study, genetic polymorphisms of these 23 autosomal STR loci were first obtained from 309 Sichuan Han individuals, southwest China. No evidence of deviation from the Hardy-Weinberg equilibrium (HWE) was identified...
September 5, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28868577/x-chromosomal-str-based-genetic-structure-of-sichuan-tibetan-minority-ethnicity-group-and-its-relationships-to-various-groups
#17
Guanglin He, Ye Li, Xing Zou, Ying Zhang, Hepei Li, Mengge Wang, Jin Wu
The X-chromosomal short tandem repeats (STRs) with more informative than autosomal STRs in some complicated biological relationships identification due to its specific mode of genetic transmission can be used as a complementary tool in forensic case practices. In this study, we presented the population genetic data of 19 X-STRs, consisting of DXS10174, DXS10075, DXS10079, DXS101, DXS10101, DXS10103, DXS10134, DXS10135, DXS10148, DXS10159, DXS10162, DXS10164, DXS6789, DXS6809, DXS7132, DXS7423, DXS7424, DXS8378, and HPRTB loci, in a sample of 235 individuals of Tibetan nationality from Sichuan province, Southwest China...
September 3, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28868569/genetic-analysis-of-12-x-str-loci-in-the-serbian-population-from-vojvodina-province
#18
Igor Veselinović, Dušan Vapa, Mihajla Djan, Nevena Veličković, Tanja Veljović, Galina Petrić
The analysis of 12 X-STR loci included in the Investigator® Argus X-12 kit was performed on a sample of 325 unrelated persons from Vojvodina Province, Republic of Serbia. No significant differences were observed in the allele frequencies in males and females. Heterozygosity values among the studied loci ranged from 67.62 to 94.28%. All loci in female individuals were consistent with the Hardy-Weinberg equilibrium test. The combined power of discrimination values in male and female individuals was 0.9999999994 and 0...
September 3, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28867528/corrigendum-to-u-s-population-data-for-29-autosomal-str-loci-forensic-sci-int-genet-7-2013-e82-e83
#19
Carolyn R Steffen, Michael D Coble, Katherine B Gettings, Peter M Vallone
No abstract text is available yet for this article.
August 12, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28866789/population-data-and-forensic-efficiency-of-21-autosomal-str-loci-included-in-agcu-ex22-amplification-system-in-the-wanzhou-han-population
#20
Xing Zou, Yongguo Li, Zehong Wei, Ting Wang, Yue Hu, Ying Zhu, Jianbo Li, Renkuan Tang
Autosomal short tandem repeats (STRs), widely distributed in the whole human genome, play an important role in personal identification and parentage testing. In this study, the allele frequencies and forensic efficiency parameters of 21 autosomal STR loci included in the AGCU EX22 kit were obtained from 433 Chinese Han individuals residing in the Wanzhou District in the north of Chongqing Municipality, Southwest China. No deviation from Hardy-Weinberg equilibrium was observed in all of the 21 STR loci. A total of 241 alleles were observed with allele frequencies ranging from 0...
September 2, 2017: International Journal of Legal Medicine
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