keyword
MENU ▼
Read by QxMD icon Read
search

Forensic genetics

keyword
https://www.readbyqxmd.com/read/27898725/spatially-explicit-models-to-investigate-geographic-patterns-in-the-distribution-of-forensic-strs-application-to-the-north-eastern-mediterranean
#1
Francesco Messina, Andrea Finocchio, Nejat Akar, Aphrodite Loutradis, Emmanuel I Michalodimitrakis, Radim Brdicka, Carla Jodice, Andrea Novelletto
Human forensic STRs used for individual identification have been reported to have little power for inter-population analyses. Several methods have been developed which incorporate information on the spatial distribution of individuals to arrive at a description of the arrangement of diversity. We genotyped at 16 forensic STRs a large population sample obtained from many locations in Italy, Greece and Turkey, i.e. three countries crucial to the understanding of discontinuities at the European/Asian junction and the genetic legacy of ancient migrations, but seldom represented together in previous studies...
2016: PloS One
https://www.readbyqxmd.com/read/27890103/the-genetic-structure-of-native-americans-in-north-america-based-on-the-globalfiler%C3%A2-strs
#2
Kelly L McCulloh, Jillian Ng, Robert F Oldt, Jessica A Weise, Joy Viray, Bruce Budowle, David Glenn Smith, Sreetharan Kanthaswamy
Current forensic STR databases, such as CODIS, lack population genetic data on Native American populations. Information from a geographically diverse array of tribes is necessary to provide improved statistical estimates of the strength of associations with DNA evidence. The Globalfiler® STR markers were used to characterize the genetic structure of ten tribal populations from seven geographic regions in North America, including those not presently represented in forensic databases. Samples from the Arctic region, Baja California, California/Great Basin, the Southeast, Mexico, the Midwest, and the Southwest were analyzed for allele frequencies, observed and expected heterozygosities, and F-statistics...
November 2016: Legal Medicine
https://www.readbyqxmd.com/read/27890101/genetic-polymorphism-studies-on-22-autosomal-str-loci-of-the-powerplex-fusion-system-in-bangladeshi-population
#3
Tania Hossain, Mahamud Hasan, Ashish K Mazumder, Pilu Momtaz, Abu Sufian, Jabedul Alam Khandaker, Sharif Akhteruzzaman
Genetic polymorphism of 22 autosomal STR loci included in PowerPlex® Fusion System (D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA and D22S1045) was studied in 188 unrelated Bangladeshi Bengali individuals. Allele frequencies and forensic efficiency parameters such as, the power of discrimination (PD), observed and expected heterozygosity (Ho & He), polymorphism information content (PIC), probability of match (PM), power of exclusion (PE) and typical paternity index was calculated for the loci...
November 2016: Legal Medicine
https://www.readbyqxmd.com/read/27890097/forensic-genetic-study-of-29-y-strs-in-korean-population
#4
Ju Yeon Jung, Ji-Hye Park, Yu-Li Oh, Han-Sol Kwon, Hyun-Chul Park, Kyung-Hwa Park, Eun Hye Kim, Dong-Sub Lee, Si-Keun Lim
In this study, we compared two recently released commercial Y-chromosomal short tandem repeat (Y-STR) kits: the PowerPlex Y23 System (PPY23) and Yfiler® Plus PCR amplification kit (YPlus). We performed validation studies, including sensitivity, tolerance to PCR inhibitors, and mixture analysis, and a population genetics study using 306 unrelated South Korean males. PPY23 and YPlus showed similar sensitivity, but PPY23 showed higher tolerance to humic acid than YPlus. Furthermore, the detection rate of unique minor alleles called from male/male mixtures was higher for PPY23 than for YPlus...
November 2016: Legal Medicine
https://www.readbyqxmd.com/read/27860446/a-genetic-algorithm-based-framework-for-wavelength-selection-on-sample-categorization
#5
Michel J Anzanello, Gabrielli Yamashita, Marcelo Marcelo, Flavio F Fogliatto, Rafael S Ortiz, Kristiane Mariotti, Marco F Ferrão
In forensic and pharmaceutical scenarios, the application of chemometrics and optimization techniques have unveiled common and peculiar features of seized medicine and drug samples, helping investigative forces towards tracking illegal operations. This paper proposes a novel framework aimed at identifying relevant subsets of attenuated total reflectance Fourier transform infrared (ATR-FTIR) wavelengths for classifying samples into two classes, e.g. authentic or forged categories in case of medicines, or salt or base form in cocaine analysis...
November 18, 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27853877/genetic-polymorphisms-of-20-autosomal-str-loci-in-the-vietnamese-population-from-yunnan-province-southwest-china
#6
Xiufeng Zhang, Liping Hu, Lei Du, Aiting Nie, Min Rao, Jing Bo Pang, Shengjie Nie
The genetic polymorphisms of 20 autosomal short tandem repeat (STR) loci included in the PowerPlex® 21 kit were evaluated in 522 healthy unrelated Vietnamese from Yunnan, China. All of the loci reached the Hardy-Weinberg equilibrium. These loci were examined to determine allele frequencies and forensic statistical parameters. The combined discrimination power and probability of excluding paternity of the 20 STR loci were 0.999999999999999999999991 26 and 0.999999975, respectively. Results suggested that the 20 STR loci are highly polymorphic, which is suitable for forensic personal identification and paternity testing...
November 16, 2016: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/27851802/str-profiling-for-discrimination-between-wild-and-domestic-swine-specimens-and-between-main-breeds-of-domestic-pigs-reared-in-belarus
#7
Krzysztof Rębała, Alina A Rabtsava, Svetlana A Kotova, Viachaslau N Kipen, Natalja V Zhurina, Alla I Gandzha, Iosif S Tsybovsky
A panel comprising 16 short tandem repeats (STRs) and a gender-specific amelogenin marker was worked out and tested for robustness in discrimination between wild and domestic swine subspecies encountered in Europe, between regional populations of wild boars and between main breeds of domestic pigs reared in Belarus. The STR dataset comprised 310 wild boars, inhabiting all administrative regions of Belarus, and 313 domestic pigs, representing three local and three cosmopolitan lines. Additionally, a total of 835 wild boars were genotyped for the presence of melanocortin 1 receptor (MC1R) alleles specific for domestic pigs...
2016: PloS One
https://www.readbyqxmd.com/read/27833167/a-30-indel-assay-for-genetic-variation-and-population-structure-analysis-of-chinese-tujia-group
#8
Chunmei Shen, Bofeng Zhu, Tianhua Yao, Zhidan Li, Yudang Zhang, Jiangwei Yan, Bo Wang, Xiaohua Bie, Fadao Tai
In the present study, thirty autosomal insertion and deletion polymorphic loci were simultaneously amplified and genotyped in a multiplex system, and their allelic frequencies as well as several forensic parameters were obtained in a sample of 236 unrelated healthy Tujia individuals. All the loci were in Hardy-Weinberg equilibrium after applying a Bonferroni correction and all pair-wise loci showed no significant linkage disequilibrium. These loci were observed to be relatively informative and discriminating, quite efficient for forensic applications...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27828728/population-differentiations-and-phylogenetic-analysis-of-tibet-and-qinghai-tibetan-groups-based-on-30-indel-loci
#9
Yuxin Guo, Chunmei Shen, Haotian Meng, Qian Dong, Tingting Kong, Chunhua Yang, Hongdan Wang, Rui Jin, Bofeng Zhu
In recent years, Insertion/Deletion (InDel) polymorphisms have become a hot area of forensic research. In this study, 30 InDel loci were selected to investigate the genetic polymorphisms of Tibetan groups, which are from Tibet Autonomous Region and Qinghai province of China, and explore the genetic relationships between Tibetan groups and other groups. Allele frequencies of the 30 InDel loci ranged from 0.1219 (HLD111) to 0.5609 (HLD57) in the Tibet Tibetan group and 0.1639 (HLD118) to 0.5655 (HLD124) in the Qinghai Tibetan group...
November 9, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27826995/molecular-diversity-of-23-y-chromosome-short-tandem-repeat-loci-in-the-population-of-tuzla-canton-bosnia-and-herzegovina
#10
Naida Babić, Serkan Dogan, Jasmina Čakar, Amela Pilav, Damir Marjanović, Vesna Hadžiavdić
BACKGROUND: Tuzla Canton is the most populated region in the ethnically mixed territory of Bosnia and Herzegovina, whose genetic analysis could provide an insight into past demographic events. AIM: Analysis of 23 Y-chromosome STR markers in the population of Tuzla Canton and investigation of the genetic relationship of the male population of the Tuzla Canton and that of the larger Bosnian and Herzegovinian population as well as neighbouring and other European populations...
November 30, 2016: Annals of Human Biology
https://www.readbyqxmd.com/read/27823806/the-forensic-use-of-behavioral-genetics-in-criminal-proceedings-case-of-the-maoa-l-genotype
#11
Sally McSwiggan, Bernice Elger, Paul S Appelbaum
The role of behavioral genetic evidence in excusing and mitigating criminal behavior is unclear. Research has suggested that a low activity genotype of the enzyme monoamine oxidase (MAOA-L) may increase the risk for aggressive and antisocial behavior. By examining criminal proceedings in which MAOA-L genotype evidence was introduced, we explored the forensic uses of behavioral genetic science. Westlaw and LexisNexis legal databases were electronically searched for cases from 1995 to 2016 to identify court documents from cases involving the MAOA-L genotype...
November 4, 2016: International Journal of Law and Psychiatry
https://www.readbyqxmd.com/read/27822853/genetic-fingerprinting-using-microsatellite-markers-in-a-multiplex-pcr-reaction-a-compilation-of-methodological-approaches-from-primer-design-to-detection-systems
#12
Jacqueline Krüger, Dorit Schleinitz
Microsatellites are polymorphic DNA loci comprising repeated sequence motifs of two to five base pairs which are dispersed throughout the genome. Genotyping of microsatellites is a widely accepted tool for diagnostic and research purposes such as forensic investigations and parentage testing, but also in clinics (e.g. monitoring of bone marrow transplantation), as well as for the agriculture and food industries. The co-amplification of several short tandem repeat (STR) systems in a multiplex reaction with simultaneous detection helps to obtain more information from a DNA sample where its availability may be limited...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27819737/human-autosomal-dna-and-x-chromosome-str-profiles-obtained-from-chrysomya-albiceps-diptera-calliphoridae-larvae-used-as-a-biological-trace
#13
T C Oliveira, A B R Santos, K C N Rabelo, C A Souza, S M Santos, S Crovella
The use of insects to answer questions in criminal investigations, as well as a combination of forensic genetic techniques to obtain human DNA from the organisms, especially necrophagous dipterians, have gained ground in recent decades among researchers and professionals in this area. The objective of our study was to evaluate and compare two methods of human DNA extraction, commonly used for forensic samples, to obtain human autosomal DNA and X chromosome short tandem repeat profiles from the digestive tract of Chrysomya albiceps (Diptera: Calliphoridae) larvae...
November 3, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27818047/advances-in-digital-polymerase-chain-reaction-dpcr-and-its-emerging-biomedical-applications
#14
REVIEW
Lei Cao, Xingye Cui, Jie Hu, Zedong Li, Jane Ru Choi, Qingzhen Yang, Min Lin, Li Ying Hui, Feng Xu
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and forensic identification. In the past three decades, PCR has evolved from end-point PCR, through real-time PCR, to its current version, which is the absolute quantitive digital PCR (dPCR). In this review, we first discuss the principles of all key steps of dPCR, i.e., sample dispersion, amplification, and quantification, covering commercialized apparatuses and other devices still under lab development...
September 25, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27817057/the-y-chromosome-as-the-most-popular-marker-in-genetic-genealogy-benefits-interdisciplinary-research
#15
REVIEW
Francesc Calafell, Maarten H D Larmuseau
The Y chromosome is currently by far the most popular marker in genetic genealogy that combines genetic data and family history. This popularity is based on its haploid character and its close association with the patrilineage and paternal inherited surname. Other markers have not been found (yet) to overrule this status due to the low sensitivity and precision of autosomal DNA for genetic genealogical applications, given the vagaries of recombination, and the lower capacities of mitochondrial DNA combined with an in general much lower interest in maternal lineages...
November 5, 2016: Human Genetics
https://www.readbyqxmd.com/read/27816850/a-multiplex-pcr-system-for-13-rm-y-strs-with-separate-amplification-of-two-different-repeat-motif-structures-in-dyf403s1a
#16
Eun Young Lee, Hwan Young Lee, So Yeun Kwon, Yu Na Oh, Woo Ick Yang, Kyoung-Jin Shin
In forensic science and human genetics, Y-chromosomal short tandem repeats (Y-STRs) have been used as very useful markers. Recently, more Y-STR markers have been analyzed to enhance the resolution power in haplotype analysis, and 13 rapidly mutating (RM) Y-STRs have been suggested as revolutionary tools that can widen Y-chromosomal application from paternal lineage differentiation to male individualization. We have constructed two multiplex PCR sets for the amplification of 13 RM Y-STRs, which yield small-sized amplicons (<400bp) and a more balanced PCR efficiency with minimum PCR cycling...
October 27, 2016: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/27816849/a-collaborative-ednap-exercise-on-snapshot%C3%A2-based-mtdna-control-region-typing
#17
N E C Weiler, K Baca, D Ballard, F Balsa, M Bogus, C Børsting, F Brisighelli, J Červenáková, L Chaitanya, M Coble, V Decroyer, S Desmyter, K J van der Gaag, K Gettings, C Haas, J Heinrich, M João Porto, A J Kal, M Kayser, A Kúdelová, N Morling, A Mosquera-Miguel, F Noel, W Parson, V Pereira, C Phillips, P M Schneider, D Syndercombe Court, M Turanska, A Vidaki, P Woliński, L Zatkalíková, T Sijen
A collaborative European DNA Profiling (EDNAP) Group exercise was undertaken to assess the performance of an earlier described SNaPshot™-based screening assay (denoted mini-mtSNaPshot) (Weiler et al., 2016) [1] that targets 18 single nucleotide polymorphism (SNP) positions in the mitochondrial (mt) DNA control region and allows for discrimination of major European mtDNA haplogroups. Besides the organising laboratory, 14 forensic genetics laboratories were involved in the analysis of 13 samples, which were centrally prepared and thoroughly tested prior to shipment...
October 24, 2016: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/27816789/identification-of-a-discordant-genotype-at-the-d2s1338-locus-between-identifiler-plus-%C3%A2-16-kit-and-power-plex-%C3%A2-21-system-kit
#18
Lin Zhang, Hongyan Wu, Kejie Wang, Aiying Fan, Jiafeng Niu, Zhendong Zhu
Genotyping errors can perturb estimates of genetic diversity and adversely affects the reliability of forensic identification, which has become increasingly recognized. During our daily laboratory work, we hit upon a discordant short tandem repeat (STR) genotype at the D2S1338 locus between Identifiler Plus(®)16 system kit and Power Plex(®)21 system kit. To figure out the reason of the discordance, nested PCR was performed to amplify the D2S1338 locus and then the final amplicons were sequenced. We found a G to G/A transition at the position on which caused an allele dropout of Identifiler Plus(®)16 kit...
October 24, 2016: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/27815639/dna-methylation-based-variation-between-human-populations
#19
REVIEW
Farzeen Kader, Meenu Ghai
Several studies have proved that DNA methylation affects regulation of gene expression and development. Epigenome-wide studies have reported variation in methylation patterns between populations, including Caucasians, non-Caucasians (Blacks), Hispanics, Arabs, and numerous populations of the African continent. Not only has DNA methylation differences shown to impact externally visible characteristics, but is also a potential biomarker for underlying racial health disparities between human populations. Ethnicity-related methylation differences set their mark during early embryonic development...
November 4, 2016: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/27810088/the-promise-and-peril-of-precision-medicine-phenotyping-still-matters-most
#20
REVIEW
Jaeger P Ackerman, Daniel C Bartos, Jamie D Kapplinger, David J Tester, Brian P Delisle, Michael J Ackerman
We illustrate the work necessary to reverse course after identification of a KCNQ1 variant interpreted erroneously as causing long QT syndrome (LQTS) and to identify the true cause of a case of sudden death in the young. Surrogate genetic testing of a decedent's living brother identified a rare KCNQ1-V133I variant, which prompted an implantable cardioverter defibrillator and subsequent diagnosis of LQTS in other family members. Subsequently, this presumed LQT1 family came to our institution for further clinical evaluation and research-based investigations, including KCNQ1-V133I variant-specific analysis of the decedent, heterologous expression studies of KCNQ1-V133I, and a whole-exome molecular autopsy along with genomic triangulation using his unaffected parents' DNA...
October 8, 2016: Mayo Clinic Proceedings
keyword
keyword
52643
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"