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Jing Wang, Weiwei Wang, Yongtao Pang
Saikosaponin A (SSA) is the major triterpenoid glycoside isolated from Bupleurum falcatum. In this study, we reported the protective effects and mechanism of SSA on lipopolysaccharide (LPS)-induced endometritis in mice. The pathological changes and myeloperoxidase (MPO) activity of uterus tissues were evaluated by hematoxylin and eosin (H&E) staining and MPO detection kit. Inflammatory cytokines TNF-α, IL-1ß, and IL-6 production were detected by ELISA. The expression of protein was measured by western blot analysis...
May 10, 2018: Inflammation
Xiao-Qin Li, Ya-Nan Song, Su-Juan Wang, Khalid Rahman, Jian-Yong Zhu, Hong Zhang
Over the past decades, a number of phytochemicals have been reported to possess potent pharmacological effects. Saikosaponins represent a group of oleanane derivatives, usually as glucosides, which are commonly found in medicinal plants Bupleurum spp., which have been used as traditional Chinese medicine for more than 1,000 years in China. Emerging evidence suggests that saikosaponins have many pharmacological effects, including sedation, anticonvulsant, antipyretic, antiviral, immunity, anti-inflammation, antitumor properties, protecting liver and kidney and so on...
May 4, 2018: Journal of Asian Natural Products Research
Haibin Tong, Xiaoli Zheng, Jianxi Song, Jian Liu, Ting Ren, Xu Zhang, Luqi Huang, Mingjiang Wu
In this study, BCPS-1, a polysaccharide previously isolated and characterized from Bupleurum chinense was chemically modified to yield two sulfated derivatives, which we designated as S-BCP1-4 and S-BCP1-8 . The physicochemical properties of these sulfated derivatives were then determined by high performance liquid chromatography (HPLC), Fourier transform infrared spectrometry (FT-IR), and gas chromatography (GC), and then compared with those of BCPS-1. Furthermore, the antioxidant activities of all three polysaccharides were also evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, superoxide radical assay and hydroxyl radical assay, while their effects against H2 O2 -induced cellular senescence were determined using senescence-associated β-galactosidase (SA-β-gal) staining, cell cycle assay and immunoblotting in H2 O2 -induced mouse lung endothelial cells (MLECs)...
July 15, 2018: Carbohydrate Polymers
Liubing Lin, Renye Que, Yanting Shen, Yirong Chen, Ni Yan, Yong Li
Saikosaponin‑d (SSd) the primary active component of triterpene saponin derived from Bupleurum falcatum L., possesses anti‑inflammatory and antioxidant properties. The present study aimed to examine the potential therapeutic effects of SSd on carbon tetrachloride (CCl4)‑induced acute hepatocellular injury in the HL‑7702 cell line and its underlying mechanisms. HL‑7702 cells were treated with SSd at different doses (0.5, 1 or 2 µmol/l). Cell viability was determined using an MTT assay. Injury was assessed by the levels of serum alanine aminotransferase (ALT) and aspartate transaminase (AST)...
April 5, 2018: Molecular Medicine Reports
Mohamed L Ashour, Fadia S Youssef, Haidy A Gad, Mahmoud Z El-Readi, Amel Bouzabata, Riham M Abuzeid, Mansour Sobeh, Michael Wink
OBJECTIVES: To explore the potential anti-inflammatory activity of Bupleurum marginatum extracts using in vitro and in vivo studies supported by virtual screening. METHODS: Antioxidant activity was assessed using the DPPH˙ and inhibition of 2-deoxyribose degradation assays. Anti-inflammatory activity was determined in vitro by measuring the suppression of prostaglandin E2 release (PGE2 ) in pancreatic cancer cells (MIA-PaCa-2) and the inhibition of 5-lipoxygenase whereas the rat paw oedema was used in vivo...
April 3, 2018: Journal of Pharmacy and Pharmacology
Xiuping Sun, Xianglei Li, Ruile Pan, Yanfeng Xu, Qiong Wang, Mingjing Song
BACKGROUND: Bupleurum yinchowense Shan et Y. Li is widely used to treat depressive and anxiety disorders for hundreds of years in China. Total saikosaponins (TSS) is the major ingredient of Bupleurum yinchowense. A-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor and subsequent mammalian target of rapamycin (mTOR) signaling is responsible for synaptic maturation and may contribution to the synaptic alteration underlying depression. The aim of the study was to investigate the antidepressant-like and anxiolytic effect of TSS in chronic corticosterone-treated mice...
April 2, 2018: BMC Complementary and Alternative Medicine
Yu-Mei Liu, An Zhou, Nian-Jun Yu, Rong-Chun Han, Wei Zhang, Yue-Jian Zhu, Yong Cao, Xiang-Yu Li, Dai-Yin Peng
Epoxy ether type and isophthalene type saponin are the main saponins of Bupleurum chinense. However,due to the difference of their UV spectrum,there is no quantitative method for simultaneous determination of these two kinds of saponins. In this paper,a dual-wavelength high performance liquid chromatography(HPLC) was developed for simultaneous determination of five saponins in epoxidized ether(saikosaponin a,c,d) and isosorbide type(saikosaponin b1,b2). The mobile phase was eluted with acetonitrile-water(0...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Zhi-De Zhang, Hong Li, Fen Wan, Xiao-Yu Su, Yan Lu, Dao-Feng Chen, Yun-Yi Zhang
The present study aimed to investigate the effects of polysaccharides extracted from Bupleurum chinense DC (BCPs) on macrophage functions. In the in vivo experiment, 1 mL of 5% sodium thioglycollate was injected into the abdomen of the mice on Day 0 and macrophages were harvested on Day 4. The macrophages were cultured in plates and treated with different concentrations of BCPs and stimulus. Effects of BCPs on macrophage functions were assessed by chemotaxis assay, phagocytosis assay and Enzyme-Linked Immunosorbent Assay (ELISA)...
December 2017: Chinese Journal of Natural Medicines
Renye Que, Yanting Shen, Jianlin Ren, Zhihui Tao, Xiaoyan Zhu, Yong Li
Saikosaponin-d (SSd) is one of the major triterpenoid saponins derived from Bupleurum falcatum L., which has been reported to possess antifibrotic activity. At present, there is little information regarding the potential target of SSd in hepatic stellate cells (HSCs), which serve an important role in excessive extracellular matrix (ECM) deposition during the pathogenesis of hepatic fibrosis. Our recent study indicated that SSd may be considered a novel type of phytoestrogen with estrogen‑like actions. Therefore, the present study aimed to investigate the effects of SSd on the proliferation and activation of HSCs, and the underlying mechanisms associated with estrogen receptors...
March 2018: International Journal of Molecular Medicine
Su Jin Kang, Young Joon Lee, Sung Gu Kang, Soyoung Cho, Wonsuck Yoon, Ji Hong Lim, Sang-Hyun Min, Tae Ho Lee, Byeong Mo Kim
Saikosaponin a (SSa), a bioactive phytochemical from Bupleurum , triggers sequential caspase-2 and caspase-8 activation, and thereby induces caspase-mediated apoptosis in human colon carcinoma (HCC) cells. However, the upstream mechanism of caspase-2 activation remains unknown. Therefore, we investigated the signaling mechanisms underlying SSa-induced caspase activation and apoptosis in HCC cells. SSa treatment triggered marked antitumor effects, especially in HCC cells, in a cell culture model and a mouse xenograft model...
November 21, 2017: Oncotarget
Hang Gao, Yanyan Song, Dongsong Li, Wei Feng, Jianguo Liu
Saikosaponin a (SSa), one of the main active components of Bupleurum falcatum , has been reported to have anti-inflammatory effect. In the present study, we investigated the anti-inflammatory effect of SSa on IL-1β-stimulated human osteoarthritis chondrocytes. The cells were pretreated with SSa 12 h before IL-1β treatment. The production of PGE2 and NO were detected by ELISA and Griess method. The levels of MMP1, MMP3, and MMP13 were measured by ELISA and qRT-PCR. The expression of NF-κB and LXRα were tested by western blot analysis...
October 24, 2017: Oncotarget
Xu Lixing, Ji Zhouye, Guo Liting, Zhang Ruyi, Qu Rong, Ma Shiping
Saikosaponin-d (SSd), one of the main constituents of the total saikosaponins extracted from Bupleurum falcatum L, possesses anti-inflammatory and anti-apoptosis effect. Recently, SSd was proved to improve depressive symptoms although exhibit hepatotoxicity in animals, but the central nervous system (CNS) toxicity of SSd remains unclear. The present study investigated the SSd-induced impairment in hippocampal cognitive function and explored the possible mechanisms involved. After intragastric administration of SSd (4mg/kg, 8mg/kg) for 7 days, the learning and memory abilities of mice were evaluated by behavioral experiments...
November 9, 2017: Toxicology Letters
Mi Hye Kwon, Jin Seok Jeong, Jayoung Ryu, Young Woong Cho, Hee Eun Kang
DA-9805 is a new botanical antiparkinson drug candidate formulated using an ethanolic extract of the root of Bupleurum falcatum, the root cortex of Paeonia suffruticosa, and the root of Angelica dahurica. In this study, a sensitive and rapid LC-MS/MS method was developed to simultaneously determine, saikosaponin a, paeonol, and imperatorin, three active/representative ingredients of DA-9805, in rat plasma. Plasma was extracted by mixture of ethyl acetate and methyl tertiary butyl ether. Chromatographic separation was carried out using a C18 column and a gradient elution of mobile phases consisting of 5mM formic acid in water and acetonitrile...
November 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Feng Zhang, Yuan Fang, Yu-Zhi Zhou, Jun-Sheng Tian, Xue-Mei Qin, Xiao-Xia Gao
To establish quantitative methods for determination of polyacetylenes in Bupleuri Radix, an ultra-performance liquid chromatography method coupled with photodiode array detector (UPLC-PDA) was developed. The analysis was performed on a Waters BEH C₁₈ column (2.1 mm×100 mm, 1.7 μm) using a gradient system of methanol and water. The flow rate was 0.3 mL•min⁻¹ and the detection wavelength was 315 nm. Eight polyacetylenes were prepared using traditional extraction and isolation method, of which compounds 7 and 8 were two new polyacetylenes...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Benedetta Fois, Giulia Bianco, Vijay P Sonar, Simona Distinto, Elias Maccioni, Rita Meleddu, Claudia Melis, Luisa Marras, Raffaello Pompei, Costantino Floris, Pierluigi Caboni, Filippo Cottiglia
The dichloromethane extract of the leaves of Bupleurum fruticosum was found to inhibit the replication of human rhinovirus (HRV) serotypes 14 and 39. Bioassay-guided fractionation led to the isolation of seven phenylpropenol derivatives (3-9), two polyacetylenes (1 and 2), and one monoterpene (10). Compounds 1 and 10 were identified as previously undescribed secondary metabolites after extensive 1D and 2D NMR experiments as well as high-resolution mass spectrometry. Compounds 2, 4, and 5 showed a selective inhibition of viral replication against HRV39 serotype, with 2 and 4 being the most active, with EC50 values of 1...
October 27, 2017: Journal of Natural Products
Xin Liu, Simone Latkolik, Atanas G Atanasov, Olaf Kunert, Eva-Maria Pferschy-Wenzig, Elke H Heiss, Clemens Malainer, Andreas Schinkovitz, Manfred Kollroser, Verena M Dirsch, Rudolf Bauer
The roots of Bupleurum chinense have a long history in traditional medicine to treat infectious diseases and inflammatory disorders. Two major compounds, saikosaponins A and D, were reported to exert potent anti-inflammatory activity by inhibiting NF- κ B. In the present study, we isolated new saikosaponin analogues from the roots of B. chinese interfering with NF- κ B activity in vitro . The methanol-soluble fraction of the dichloromethane extract of Radix Bupleuri was subjected to activity-guided isolation yielding 18 compounds, including triterpenoids and polyacetylenes...
October 2017: Planta Medica
Chisato Morino, Yusuke Morita, Kazuki Minami, Yuto Nishidono, Yoshitaka Nakashima, Rika Ozawa, Junji Takabayashi, Naoaki Ono, Shigehiko Kanaya, Takayuki Tamura, Yasuhiro Tezuka, Ken Tanaka
Umbelliferous medicinal plants, such as Angelica acutiloba Kitagawa and Angelica dahurica Bentham et Hooker filius ex Franchet et Savatier, account for a large percentage of crude drug consumption in Japan. The most serious problem in the cultivation of umbelliferous medicinal plants is the feeding damage caused by the common yellow swallowtail (Papilio machaon hippocrates C. & R. Felder, 1864). When we compared the numbers of eggs laid by P. machaon on six umbelliferous medicinal plants, the eggs on A...
January 2018: Journal of Natural Medicines
Yi-Rong Lin, Mei-Yao Wu, Jen-Huai Chiang, Hung-Rong Yen, Su-Tso Yang
BACKGROUND: Many patients with gynecological disorders seek traditional medicine consultations in Asian countries. This study intended to investigate the utilization of traditional Chinese medicine (TCM) in patients with dysfunctional uterine bleeding (DUB) in Taiwan. METHODS: We analyzed a cohort of one million individuals randomly selected from the National Health Insurance Research Database in Taiwan. We included 46,337 subjects with newly diagnosed DUB (ICD-9-CM codes 626...
August 29, 2017: BMC Complementary and Alternative Medicine
Xiao-Xiao Lu, Yi-Fan Jiang, Hong Li, Ying-Ye Ou, Zhi-De Zhang, Hong-Ye DI, Dao-Feng Chen, Yun-Yi Zhang
Lipopolysaccharides (LPS) contamination in herbal crude polysaccharides is inevitable. The present study was performed to explore the effect of polymyxin B on abolishing the influence of LPS contamination in mononuclear cells. LPS was pretreated with polymyxin B sulfate (PB) at different concentrations for 1, 5 or 24 h, and then used to stimulate RAW264.7 and mouse peritoneal macrophages (MPMs). The nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in cell culture supernatant, as the indications of cell response, were assayed...
July 2017: Chinese Journal of Natural Medicines
Yue-Xin Wang, Qing-Yang Liu, Mei Zhang, Zhao Yang, Xin Pei, Xu Wu, Xin Chen, Jun Hong, Kai-Zhi Xu
BACKGROUND: Bupleurum chinense, a well-known Traditional Chinese Medicine, has been used for thousands of years in China. In this study, we would suggest that Bupleurum polysaccharides (BPS) could improve the prognosis of sepsis through its impact on redistribution of BMCs, which triggers immune reversal in late sepsis. METHODS: BALB/c mice were divided into five groups: sham burn group, burn plus P aeruginosa group, burn plus P aeruginosa with BPS (40 mg/kg, 100 mg/kg, and 250 mg/kg) treatment group, and they were sacrificed at post-burn day (PBD) 0, 3, 5, and 7...
April 2018: Shock
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