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https://www.readbyqxmd.com/read/29778469/communications-of-staphylococcus-aureus-and-non-aureus-staphylococcus-species-from-bovine-intramammary-infections-and-teat-apex-colonization
#1
Yasser S Mahmmod, Ilka Christine Klaas, Line Svennesen, Karl Pedersen, Hanne Ingmer
The role of non-aureus staphylococci (NAS) in the risk of acquisition of intramammary infections with Staphylococcus aureus is vague and still under debate. The objectives of this study were to (1) investigate the distribution patterns of NAS species from milk and teat skin in dairy herds with automatic milking systems, and (2) examine if the isolated NAS influences the expression of S. aureus virulence factors controlled by the accessory gene regulator (agr) quorum sensing system. In 8 herds, 14 to 20 cows with elevated somatic cell count were randomly selected for teat skin swabbing and aseptic quarter foremilk samples from right hind and left front quarters...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29777793/homocysteine-induces-vascular-inflammatory-response-via-smad7-hypermethylation-in-human-umbilical-vein-smooth-muscle-cells
#2
Li-Hua Wei, Nai-Xia Chao, Si Gao, Ya-Ting Yu, Lin Shi, Xin-Bo Ma, Na Liao, Ke Lan, Yu Luo, Zheng-Yi Xie, Yuan-Yuan Li
Homocysteine (Hcy) can induce atherosclerosis through the inflammatory response and DNA methylation disorder. Our recent study has reported a novel epigenetic modified gene related to atherosclerosis -SMAD7. To further understand the pathogenesis of atherosclerosis, the current study was designed to investigate an inflammatory role of Hcy in human umbilical vein smooth muscle cells (HUVSMCs) through interfering with SMAD7 methylation. Using MALDI-TOF MS, we found that Hcy increased DNA methylation levels of SMAD7 promoter in a dose and time-dependent manner in HUVSMCs...
May 16, 2018: Microvascular Research
https://www.readbyqxmd.com/read/29777688/proteomic-changes-during-adult-stage-in-pre-optic-hypothalamus-hippocampus-and-pituitary-regions-of-female-rat-brain-following-neonatal-exposure-to-estradiol-17%C3%AE
#3
Vijayakumar Govindaraj, Radhika Nagamangalam Shridharan, Addicam Jagannadha Rao
Although neonatal exposure to estrogen or estrogenic compounds results in irreversible changes in the brain function and reproductive abnormalities during adulthood but the underlying mechanisms are still largely unknown. The present study has attempted to compare the protein profiles of sexually dimorphic brain regions of adult female rats which were exposed to estradiol- 17β during neonatal period. The total proteins extracted from pre-optic area (POA), hypothalamus, hippocampus and pituitary of control and neonatally E2 treated female rats was subjected to 2D-SDS-PAGE and differentially expressed proteins were identification by MALDI TOF/TOF-MS...
May 16, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29777151/integrated-proteomics-genomics-metabolomics-approaches-reveal-oxalic-acid-as-pathogenicity-factor-in-tilletia-indica-inciting-karnal-bunt-disease-of-wheat
#4
Vishakha Pandey, Manoj Singh, Dinesh Pandey, Anil Kumar
Tilletia indica incites Karnal bunt (KB) disease in wheat. To date, no KB resistant wheat cultivar could be developed due to non-availability of potential biomarkers related to pathogenicity/virulence for screening of resistant wheat genotypes. The present study was carried out to compare the proteomes of T. indica highly (TiK) and low (TiP) virulent isolates. Twenty one protein spots consistently observed as up-regulated/differential in the TiK proteome were selected for identification by MALDI-TOF/TOF. Identified sequences showed homology with fungal proteins playing essential role in plant infection and pathogen survival, including stress response, adhesion, fungal penetration, invasion, colonization, degradation of host cell wall, signal transduction pathway...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29776363/rapid-detection-of-carbapenemase-activity-of-enterobacteriaceae-isolated-from-positive-blood-cultures-by-maldi-tof-ms
#5
Jiajia Yu, Jingxian Liu, Yuanrui Li, Jing Yu, Weinan Zhu, Ying Liu, Lisong Shen
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical microbiology laboratories. β-Lactam antibiotics are commonly used for treatment of bloodstream infections caused by Enterobacteriaceae strains, and carbapenem is the superlative class of β-lactam antibiotics. Since the carbapenem resistance rate of Enterobacteriaceae strains raised year by year, efficient detection of carbapenemase activity and timely delivery of carbapenem susceptibility reports of Enterobacteriaceae strains isolated from blood cultures is important for clinicians...
May 18, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29775706/purification-and-characterization-of-an-organic-solvent-tolerant-and-detergent-stable-lipase-from-haloferax-mediterranei-cncmm-50101
#6
Siham Akmoussi-Toumi, Souad Khemili-Talbi, Imen Ferioune, Salima Kebbouche-Gana
The present study investigates the purification and biochemical characterization of an extracellular lipase (HML) from Haloarchaea Haloferax mediterranei strain ATS1, isolated from the Sebkha (Medea, Algeria). The pure protein was obtained with ammonium sulfate precipitation (40-70%)-dialysis and UNO Q-6 FPLC, and characterized biochemically. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis revealed that the purified enzyme was a monomer, with a molecular mass of 45,011...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775704/alkaline-active-cyanide-dihydratase-of-flavobacterium-indicum-mtcc-6936-growth-optimization-purification-characterization-and-in-silico-analysis
#7
Virender Kumar, Vijay Kumar, Tek Chand Bhalla
The present work explores a rare cyanide dihydratase of Flavobacterium indicum MTCC 6936 for its potential of cyanide degradation. The enzyme is purified to 12 fold with a yield of 76%. SDS and native-PAGE analysis revealed that enzyme was monomer of 40 kDa size. The enzyme works well in mesophilic range at wide array of pH. The thermostability profile of cyanide dihydratase revealed that the enzyme is quite stable at 30 °C and 35 °C with half-life of 6 h 30 min and 5 h respectively. Km and Vmax for cyanide dihydratase of F...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29774958/effect-of-trapping-method-on-species-identification-of-phlebotomine-sandflies-by-maldi-tof-ms-protein-profiling
#8
P Halada, K Hlavackova, J Risueño, E Berriatua, P Volf, V Dvorak
Sandflies (Diptera: Psychodidae) (Newstead, 1911) are blood-feeding insects that transmit human pathogens including Leishmania (Trypanosomatida: Trypanosomatidae) parasites, causative agents of the leishmaniases. To elucidate Leishmania transmission cycles, conclusive identification of vector species is essential. Molecular approaches including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) protein profiling have recently emerged to complement morphological identification...
May 18, 2018: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/29774329/syntheses-and-studies-of-electron-energy-transfer-of-new-dyads-based-on-an-unsymmetrical-perylene-diimide-incorporating-chelating-1-10-phenanthroline-and-its-corresponding-square-planar-complexes-with-dichloroplatinum-ii-and-dichloropalladium-ii
#9
Sebile Işık Büyükekşi, Ahmet Karatay, Nursel Acar, Betül Küçüköz, Ayhan Elmali, Abdurrahman Şengül
Perylene diimides (PDIs) are among the most versatile and functional dyes for supramolecular structures displaying characteristic high absorptions and photo-luminescence properties as the prerequisite for optoelectronic thin film devices. Despite intense investigations into these semi-conducting and electro-active materials, details of their electronic structure are still under examination. In particular, non-planar twisted PDIs as an electron acceptor is a promising model system for efficient charge generation and transport processes...
May 18, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29773373/environmentally-friendly-pathways-towards-the-synthesis-of-vinyl-based-oligocelluloses
#10
Azis Adharis, Dejan M Petrović, Ibrahim Özdamar, Albert J J Woortman, Katja Loos
The synthesis of vinyl-based oligocelluloses using cellodextrin phosphorylase as biocatalyst in buffer solution is presented. Various types of vinyl glucosides bearing (meth)acrylates/(meth)acrylamides functionalities served as the glucosyl acceptor in the enzyme catalyzed reverse phosphorolysis reaction and α-glucose 1-phosphate as the glucosyl donor. The enzymatic reaction was followed by thin layer chromatography and the isolated product yields were about 65%. The synthesized vinyl-based oligocelluloses had an average number of repeating glucosyl units and a number average molecular weight up to 8...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29770892/identification-of-n-terminally-truncated-derivatives-of-insulin-analogs-formed-in-pharmaceutical-formulations
#11
Joanna Zielińska, Jacek Stadnik, Anna Bierczyńska-Krzysik, Dorota Stadnik
PURPOSE: Isolation and identification of unknown impurities of recombinant insulin lispro (produced at IBA) formed during accelerated stability testing of pharmaceutical solutions. For comparative purposes also commercially available formulations of recombinant human insulin (Humulin S®; Lilly), recombinant insulin lispro (Humalog®; Lilly), recombinant insulin aspart (NovoRapid® Penfill®; Novo Nordisk), recombinant insulin detemir (Levemir®; Novo Nordisk) and recombinant insulin glargine (Lantus®; Sanofi-Aventis) were analyzed...
May 16, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29765361/biodiversity-of-environmental-leptospira-improving-identification-and-revisiting-the-diagnosis
#12
Roman Thibeaux, Dominique Girault, Emilie Bierque, Marie-Estelle Soupé-Gilbert, Anna Rettinger, Anthony Douyère, Michael Meyer, Gregorio Iraola, Mathieu Picardeau, Cyrille Goarant
Leptospirosis is an important environmental disease and a major threat to human health causing at least 1 million clinical infections annually. There has recently been a growing interest in understanding the environmental lifestyle of Leptospira . However, Leptospira isolation from complex environmental samples is difficult and time-consuming and few tools are available to identify Leptospira isolates at the species level. Here, we propose a polyphasic isolation and identification scheme, which might prove useful to recover and identify environmental isolates and select those to be submitted to whole-genome sequencing...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29764754/detection-of-methicillin-resistant-coagulase-negative-staphylococci-harboring-the-class-a-mec-complex-by-maldi-tof-mass-spectrometry
#13
Dominik Schuster, Michaele Josten, Kathrin Janssen, Isabel Bodenstein, Cathrin Albert, Anna Schallenberg, Mike Gajdiss, Esther Sib, Christiane Szekat, Katja Kehl, Marijo Parčina, Gunnar T R Hischebeth, Gabriele Bierbaum
The aim of this study was to test the identification of methicillin resistance in coagulase-negative staphylococci by routine matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). SCCmec cassettes of type II, III and VIII encode a small peptide called PSM-mec in the vicinity of mecA. It is visible at m/z 2415 during MALDI-TOF MS of whole cells of Staphylococcus aureus. In view of the fact that psm-mec has been identified in methicillin-resistant coagulase-negative staphylococci, we evaluated a collection of clinical coagulase-negative staphylococci, that contained 77...
May 8, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29762871/fast-identification-of-dermatophytes-by-maldi-tof-ms-using-direct-transfer-of-fungal-cells-on-ground-steel-target-plates
#14
Keith C da Cunha, Arnaud Riat, Anne-Cecile Normand, Philipp P Bosshard, Margarete T G de Almeida, Renaud Piarroux, Jacques Schrenzel, Lionel Fontao
BACKGROUND: Dermatophytes cause human infections limited to keratinized tissues. We showed that the direct transfer method allows reliable identification of non-dermatophytes mould and yeast by MALDI-TOF/MS. OBJECTIVES: We aimed at assessing whether the direct transfer method can be used for dermatophytes and whether an own mass spectra library would be superior to the Bruker library. METHODS: We used the Bruker Biotyper to build a dermatophyte mass spectra library and assessed its performance by 1/ testing a panel of mass spectrum produced with strains genotypically identified and, 2/ comparing MALDI-TOF/MS identification to morphology-based methods...
May 15, 2018: Mycoses
https://www.readbyqxmd.com/read/29762699/cultivable-bacteria-from-pectinatella-magnifica-and-the-surrounding-water-in-south-bohemia-indicate-potential-new-gammaproteobacterial-betaproteobacterial-and-firmicutes-taxa
#15
Hana Salmonová, Jirí Killer, Vera Bunešová, Martina Geigerová, Eva Vlková
Pectinatella magnifica is a freshwater bryozoan, which has become a subject of scientific interest because of its invasive expansion worldwide. To obtain a comprehensive overview of its influence on environments, information on associated bacteria is needed. In this study, cultivable bacteria associated with P. magnifica were investigated. In total, 253 isolates were selected for preliminary identification by MALDI-TOF mass spectrometry and clustered based on REP-PCR profiles. Among these, 169 strains were selected and identified using 16S rRNA gene comparative analyses...
May 14, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29762695/development-and-validation-of-an-extended-database-for-yeast-identification-by-maldi-tof-ms-in-argentina
#16
Constanza Giselle Taverna, Mariana Mazza, Nadia Soledad Bueno, Christian Alvarez, Susana Amigot, Mariana Andreani, Natalia Azula, Rubén Barrios, Norma Fernández, Barbara Fox, Liliana Guelfand, Ivana Maldonado, Omar Alejandro Murisengo, Silvia Relloso, Matias Vivot, Graciela Davel
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has revolutionized the identification of microorganisms in clinical laboratories because it is rapid, relatively simple to use, accurate, and can be used for a wide number of microorganisms. Several studies have demonstrated the utility of this technique in the identification of yeasts; however, its performance is usually improved by the extension of the database. Here we developed an in-house database of 143 strains belonging to 42 yeast species in the MALDI Biotyper platform, and we validated the extended database with 388 regional strains and 15 reference strains belonging to 55 yeast species...
May 11, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29758474/purification-and-characterization-of-two-pathogenesis-related-class-10-protein-isoforms-with-ribonuclease-activity-from-the-fresh-angelica-sinensis-roots
#17
Jianru Pan, Xiangling Wang, Lingling Li, Xian Li, Xiaoqiang Ye, Di Lv, Cuihuang Chen, Shutao Liu, Huocong He
In this study, two pathogenesis-related (PR) class 10 protein isoforms, ASPR-1 and ASPR-2, were purified from fresh roots of the Chinese medicinal plant Angelica sinensis (A. sinensis) using 80% ammonium sulfate precipitation, Sephadex G50 gel filtration chromatography, and DEAE-Sepharose ion-exchange chromatography. The molecular masses of ASPR-1 and ASPR-2 were estimated to be 16.66 kDa and 16.46 kDa, respectively, using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The isoforms are both glycoproteins containing glycosyl contents of 1...
April 30, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29758349/habitual-aerobic-exercise-gene-apoa5-named-rs662799-snp-and-response-of-blood-lipid-and-lipoprotein-phenotypes-among-older-chinese-adult
#18
Xiangyun Liu, Guoyuan Huang, Zhanbin Niu, Yuqin Wei, Ru Wang
BACKGROUND: The genetic component of dyslipidemia has been studied in adults but little in older population. It is remains unknown regarding influence and interaction of APOA5 gene single nucleotide polymorphism (SNP) and habitual aerobic exercise (HAE) on changes of blood lipids and lipoprotein phenotypes in older Chinese adults. METHODS: Four-hundred-twenty-three old Chinese individuals with HAE were divided into hyperlipidemia and normal groups. We genotyped polymorphic loci using matrix assisted laser desorption ionization time of flight mass spectrometry detection technology (MALDI-TOF)...
May 11, 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29758020/defining-icr-mo-an-intrinsic-colistin-resistance-determinant-from-moraxella-osloensis
#19
Wenhui Wei, Swaminath Srinivas, Jingxia Lin, Zichen Tang, Shihua Wang, Saif Ullah, Vishnu Goutham Kota, Youjun Feng
Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella species are a putative reservoir for MCR-1/2 genetic determinants. Here, we report the functional definition of ICR-Mo from M. osloensis, a chromosomally encoded determinant of colistin resistance, in close relation to current MCR-1/2 family...
May 14, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29757646/inhibition-of-breast-cancer-cell-migration-by-cyclotides-isolated-from-pombalia-calceolaria
#20
Meri Emili F Pinto, Jhenny Z G Najas, Luma G Magalhães, Antonio F Bobey, Jacqueline N Mendonça, Norberto P Lopes, Flávia M Leme, Simone P Teixeira, Marcelo Trovó, Adriano D Andricopulo, Johannes Koehbach, Christian W Gruber, Eduardo Maffud Cilli, Vanderlan S Bolzani
Two new bracelet cyclotides from roots of Pombalia calceolaria with potential anticancer activity have been characterized in this work. The cyclotides Poca A and B (1 and 2) and the previously known CyO4 (3) were de novo sequenced by MALDI-TOF/TOF mass spectrometry (MS). The MS2 spectra were examined and the amino acid sequences were determined. The purified peptides were tested for their cytotoxicity and effects on cell migration of MDA-MB-231, a triple-negative breast cancer cell line. The isolated cyclotides reduced the number of cancer cells by more than 80% at 20 μM, and the concentration-related cytotoxic responses were observed with IC50 values of 1...
May 14, 2018: Journal of Natural Products
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