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https://www.readbyqxmd.com/read/29350905/ampulexins-a-new-family-of-peptides-in-venom-of-the-emerald-jewel-wasp-ampulex-compressa
#1
Eugene Moore, Ryan Arvidson, Christopher Banks, Jean Urenda, Elizabeth Duong, Haroun Mohammad, Michael E Adams
The parasitoid wasp Ampulex compressa injects venom directly into the brain and subesophageal ganglion of the cockroach Periplaneta americana, inducing a seven to ten day lethargy termed hypokinesia. Hypokinesia presents as a significant reduction in both escape response and spontaneous walking. We examined aminergic and peptidergic components of milked venom with HPLC and MALDI-TOF mass spectrometry. HPLC coupled with electrochemical detection confirmed presence of dopamine in milked venom, while mass spectrometry revealed that the venom gland and venom sac have distinct peptide profiles, with milked venom predominantly composed of venom sac peptides...
January 19, 2018: Biochemistry
https://www.readbyqxmd.com/read/29350051/hsa-based-multi-target-combination-therapy-regulating-drugs-release-from-hsa-and-overcoming-single-drug-resistance-in-a-breast-cancer-model
#2
Yi Gou, Zhenlei Zhang, Dongyang Li, Lei Zhao, Meiling Cai, Zhewen Sun, Yongping Li, Yao Zhang, Hamid Khan, Hongbing Sun, Tao Wang, Hong Liang, Feng Yang
Multi-drug delivery systems, which may be promising solution to overcome obstacles, have limited the clinical success of multi-drug combination therapies to treat cancer. To this end, we used three different anticancer agents, Cu(BpT)Br, NAMI-A, and doxorubicin (DOX), to build human serum albumin (HSA)-based multi-drug delivery systems in a breast cancer model to investigate the therapeutic efficacy of overcoming single drug (DOX) resistance to cancer cells in vivo, and to regulate the drugs' release from HSA...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29350038/efficient-mass-spectral-analysis-of-active-transporters-overexpressed-in-escherichia-coli
#3
Mamiyo Kawakami, Narinobu Juge, Yuri Kato, Hiroshi Omote, Yoshinori Moriyama, Takaaki Miyaji
Structural analysis of purified active membrane proteins can be performed by mass spectrometry (MS). However, no large-scale expression systems for active eukaryotic membrane proteins are available. Moreover, because membrane proteins cannot easily be digested by trypsin and ionized, they are difficult to analyze by MS. Here, we developed a method for mass spectral analysis of eukaryotic membrane proteins combined to an overexpression system in Escherichia coli. Vesicular glutamate transporter 2 (VGLUT2/SLC17A6) with a soluble α-helical protein and histidine tag on the N- and C-terminus, respectively, was overexpressed in E...
January 19, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29349874/assessment-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-of-the-effects-of-preanalytical-variables-on-serum-peptidome-profiles-following-long-term-sample-storage
#4
Sachio Tsuchida, Mamoru Satoh, Hiroshi Umemura, Kazuyuki Sogawa, Masaki Takiwaki, Takayuki Ishige, Yui Miyabayashi, Yuuya Iwasawa, Sohei Kobayashi, Minako Beppu, Motoi Nishimura, Yoshio Kodera, Kazuyuki Matsushita, Fumio Nomura
PURPOSE: Human serum and plasma are often used as clinical specimens in proteomics analyses, and peptidome profiling of human serum is a promising tool for identifying novel disease-associated biomarkers. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used for peptidomic biomarker discovery. Careful sample collection and handling are required as either can have a profound impact on serum peptidome patterns, yet the effects of preanalytical variables on serum peptidome profiles have not been completely elucidated...
January 19, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29349780/protective-effect-of-bled-exposed-conditioned-media-on-cell-injury
#5
Phil-Sun Oh, Eun-Mi Kim, Minjoo Kim, In Sun Kim, Yeon-Hee Han, SeokTae Lim, Myung-Hee Sohn, Myoung-Hwan Ko, Hwan-Jeong Jeong
Previous studies have reported that 450 nm blue light emitting diode (BLED) induces apoptosis through a mitochondria-mediated pathway in cancer cells and reduces the early stage tumor growth. The present study was performed to determine the effects of BLED-irradiated cell metabolites on cell injury. Our results showed that conditioned medium (CM) from cells irradiated with low-dose BLED (LCM) inhibited apoptosis and increased cell survival. Cell protection-related proteins were identified in cell metabolites of CM and LCM using 2-DE and MALDI-TOF analysis...
January 19, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29349714/dbda-as-a-novel-matrix-for-the-analyses-of-small-molecules-and-quantification-of-fatty-acids-by-negative-ion-maldi-tof-ms
#6
Ling Ling, Ying Li, Sheng Wang, Liming Guo, Chunsheng Xiao, Xuesi Chen, Xinhua Guo
Matrix interference ions in low mass range has always been a concern when using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze small molecules (<500 Da). In this work, a novel matrix, N1,N4-dibenzylidenebenzene-1,4-diamine (DBDA) was synthesized for the analyses of small molecules by negative ion MALDI-TOF MS. Notably, only neat ions ([M-H]-) of fatty acids without matrix interference appeared in the mass spectra and the limit of detection (LOD) reached 0...
January 18, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29348923/cervicofacial-lymphadenitis-due-to-mycobacterium-mantenii-rapid-and-reliable-identification-by-maldi-tof-ms
#7
T Nebreda Mayoral, A G Andrés Andrés, S Fuentes Carretero, R Calleja Fernández, M S Jiménez Pajares
No abstract text is available yet for this article.
March 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29345567/proteomic-analysis-of-vernalization-responsive-proteins-in-winter-wheat-jing841
#8
Yalan Feng, Beibei Kong, Jun Zhang, Xianni Chen, Jiale Yuan, Xingwang Tang, Chao Ma
Vernalization is one of the pivotal ways for plants to flower. As one of the major cereal crops, wheat is widely planted in the world. In the present study, we applied two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) to analyze the changes in protein expression profiles in responding to vernalization in leaves of wheat seedling before (0d) and after (30d) of vernalization. The results indicated that among the 68 differentially expressed proteins that were successfully identified under vernalization, 36 were up-regulated whereas 32 were down-regulated at 0 d, respectively...
January 17, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29345117/candida-auris-a-systematic-review-and-meta-analysis-of-current-updates-on-an-emerging-multidrug-resistant-pathogen
#9
John Osei Sekyere
From 2009, Candida auris has emerged as a multidrug-resistant ascomycete yeast pathogen with the capacity for easy transmission between patients and hospitals, as well as persistence on environmental surfaces. Its association with high mortalities, breakthrough and persistent candidaemia, inconsistencies in susceptibility testing results, misidentification by available commercial identification systems and treatment failure, complicates its management and detection. Within the last nine years, C. auris has been increasingly reported from far-Eastern Asia, the Middle East, Africa, Europe, South and North America with substantial fatalities and misidentification...
January 18, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29344802/protein-profiling-of-acanthamoeba-species-using-maldi-tof-ms-for-specific-identification-of-acanthamoeba-genotype
#10
Kirti Megha, Megha Sharma, Amit Gupta, Rakesh Sehgal, Sumeeta Khurana
Acanthamoeba spp. are ubiquitous in the environment and have the potential to cause severe infections. The different genotypes of Acanthamoeba have been shown to influence the severity of the disease and response to therapy. Characterizing Acanthamoeba spp. upto genotype can aid in infection control practices. Twenty-five Acanthamoeba isolates, characterized by 18S rDNA sequencing, were subjected to MALDI-TOF MS analysis by creating a database for the individual genotypes. The differentiating features of the various spectra were observed; the coded samples were then tested against the created database...
January 17, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29343697/effluent-and-serum-protein-n-glycosylation-is-associated-with-inflammation-and-peritoneal-membrane-transport-characteristics-in-peritoneal-dialysis-patients
#11
Evelina Ferrantelli, Karima Farhat, Agnes L Hipgrave Ederveen, Karli R Reiding, Robert H J Beelen, Frans J van Ittersum, Manfred Wuhrer, Viktoria Dotz
Mass spectrometric glycomics was used as an innovative approach to identify biomarkers in serum and dialysate samples from peritoneal dialysis (PD) patients. PD is a life-saving treatment worldwide applied in more than 100,000 patients suffering from chronic kidney disease. PD treatment uses the peritoneum as a natural membrane to exchange waste products from blood to a glucose-based solution. Daily exposure of the peritoneal membrane to these solutions may cause complications such as peritonitis, fibrosis and inflammation which, in the long term, lead to the failure of the treatment...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29340859/comparative-proteomics-of-chromium-transformed-beas-2b-cells-by-2d-dige-and-maldi-tof-tof-ms
#12
Jian Lu, Miaomiao Tang, Yi Liu, Jin Wang, Zhanao Wu
Chromium (Cr) is a highly toxic, common heavy metal used in industrial production. There are two types of Cr in nature: hexavalent chromium (Cr(VI)) and chromium trichloride (Cr(III)). Cr(III) is involved in the metabolism of sugars and lipids, whereas Cr(VI) is absorbed through the respiratory tract and skin and generates free radicals that result in secondary toxicity. Cr(VI) leads to cancer in the occupational population and is therefore recognized as a human carcinogen by the International Agency for Research on Cancer...
January 16, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29339256/the-pbii-gene-of-the-human-salivary-proline-rich-protein-p-b-produces-another-protein-q504x8-with-an-opiorphin-homolog-qrgpr
#13
Eiichi Saitoh, Takuya Sega, Akane Imai, Satoko Isemura, Tetsuo Kato, Akihito Ochiai, Masayuki Taniguchi
OBJECTIVES: The NCBI gene database and human-transcriptome database for alternative splicing were used to determine the expression of mRNAs for P-B (SMR3B) and variant form of P-B. The translational product from the former mRNA was identified as the protein named P-B, whereas that from the latter has not yet been elucidated. In the present study, we investigated the expression of P-B and its variant form at the protein level. DESIGN: To identify the variant protein of P-B, (1) cationic proteins with a higher isoelectric point in human pooled whole saliva were purified by a two dimensional liquid chromatography; (2) the peptide fragments generated from the in-solution of all proteins digested with trypsin separated and analyzed by MALDI-TOF-MS; and (3) the presence or absence of P-B in individual saliva was examined by 15% SDS-PAGE...
January 10, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29337913/proteomic-analysis-and-identification-of-possible-allergenic-proteins-in-mature-pollen-of-populus-tomentosa
#14
Liuqiang Wang, Xiaoling Zhang, Jin Zhang, Wei Fan, Mengzhu Lu, Jianjun Hu
Pollen grains from Populus tomentosa, a widely cultivated tree in northern area of China, are considered to be an important aeroallergen causing severe allergic diseases. To gain insight into their allergenic components, mature Populus tomentosa pollen proteins were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were resolved on pH 4-7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified from 242 spots analyzed...
January 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29337627/increased-incidence-of-enterococcal-infection-in-nonviable-broiler-chicken-embryos-in-western-canadian-hatcheries-as-detected-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#15
Ruwani Karunarathna, Shelly Popowich, Morgan Wawryk, Betty Chow-Lockerbie, Khawaja Ashfaque Ahmed, Chenfang Yu, Mengying Liu, Kalhari Goonewardene, Thushari Gunawardana, Shanika Kurukulasuriya, Ashish Gupta, Philip Willson, Neil Ambrose, Musangu Ngeleka, Susantha Gomis
The emergence of enterococcal infections in neonatal broiler chickens in the poultry industry has become common in many countries, including Canada. The objective of this study was to examine the bacterial infections in nonviable broiler chicken embryos in three western Canadian poultry hatcheries using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The pattern of embryo mortality that occurred during incubation and the breakout analysis results were similar in all three hatcheries...
December 2017: Avian Diseases
https://www.readbyqxmd.com/read/29337283/variability-studies-of-allochthonous-stone-pine-pinus-pinea-l-plantations-in-chile-through-nut-protein-profiling
#16
V Loewe, R M Navarro-Cerrillo, R Sánchez Lucas, F J Ruiz Gómez, J Jorrín-Novo
Stone pine (Pinus pinea) is characterized by low differentiation of growth parameters, high phenotypic plasticity and low genetic variability; detecting its diversity in introduced Chilean populations is therefore relevant for conservation and breeding programs. Here, variability among allochthonous Stone pine populations in Chile was explored using electrophoresis-based proteomic analysis of pine nuts. Cones from 30 populations distributed along a climatic gradient in Chile were surveyed and sampled, and proteins were extracted from seed flour using the TCA-acetone precipitation protocol...
January 11, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29337282/comparative-proteome-analysis-of-monolayer-and-spheroid-culture-of-canine-osteosarcoma-cells
#17
Christiane Gebhard, Ingrid Miller, Karin Hummel, Martina Neschi Née Ondrovics, Sarah Schlosser, Ingrid Walter
Osteosarcoma is an aggressive bone tumor with high metastasis rate in the lungs and affects both humans and dogs in a similar way. Three-dimensional tumor cell cultures mimic the in vivo situation of micro-tumors and metastases and are therefore better experimental in vitro models than the often applied two-dimensional monolayer cultures. The aim of the present study was to perform comparative proteomics of standard monolayer cultures of canine osteosarcoma cells (D17) and three-dimensional spheroid cultures, to better characterize the 3D model before starting with experiments like migration assays...
January 11, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29336551/first-gut-instincts-are-always-right-the-resolution-required-for-a-mass-defect-analysis-of-polymer-ions-can-be-as-low-as-oligomeric
#18
Thierry Nicolas Jean Fouquet, Takaya Satoh, Hiroaki Sato
Its recent adaptation to low-resolution mass spectra of polymers using fractional base units raises the question of the minimal resolution needed for a Kendrick mass defect (KMD) analysis. Intuiting an oligomeric resolution since the mass of a repeat unit is the sole value to be known, it is challenged by the relative failure of the KMD plots computed from an isotopically resolved MALDI-TOF mass spectrum to display clear alignments in the high mass range. Another procedure based on the remainders of Kendrick mass (RKMs) overcomes this pitfall with oligomers perfectly aligned in a new RKM plot...
January 16, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29336243/identification-of-in-vitro-red-fluorescent-protein-with-antipathogenic-activity-from-the-midgut-of-the-silkworm-bombyx-mori-l
#19
Manjunatha Ks Gowda, Anitha Peter, Mahantesha Bn Naika, P Niranjana, P Shamprasad
Biotic stress affects all organisms, including the silkworm (Bombyx mori L.) which also has a plethora of defense mechanisms against the infecting pathogens. The midgut plays an important role as a natural barrier and source of innate immunity. The present investigation was thus undertaken to identify an antiviral red fluorescent protein formed in the midgut of the silkworm Bombyx mori L. The digestive juice from fifth instar Kolar Gold silkworm larvae was collected and subjected to 40 % ammonium sulfate precipitation and purified by gel filtration chromatography (GFC)...
January 15, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29335835/etiology-of-invasive-candidosis-agents-in-russia-a-multicenter-epidemiological-survey
#20
N V Vasilyeva, E R Raush, M V Rudneva, T S Bogomolova, A E Taraskina, Yong Fang, Fengmin Zhang, N N Klimko
A multicenter prospective epidemiological survey on the etiologic agents of invasive candidosis was conducted in Russia in the period of 2012-2014. Samples were collected from 284 patients with invasive candidosis and Candida species isolated by culture. The species were identified by DNA sequencing and MALDI-TOF massspectrometry. A total of 322 isolates were recovered, in which 96% of Сandida species belonged to six major species, namely, C. albicans (43.2%), C. parapsilosis (20.2%), C. glabrata (11.5%), C...
January 16, 2018: Frontiers of Medicine
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