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https://www.readbyqxmd.com/read/27921389/superiority-of-sds-lysis-over-saponin-lysis-for-direct-bacterial-identification-from-positive-blood-culture-bottle-by-maldi-tof-ms
#1
Yvan Caspar, Cécile Garnaud, Mariya Raykova, Sébastien Bailly, Marie Bidart, Danièle Maubon
PURPOSE: Fast species diagnosis has an important health care impact, as rapid and specific antibacterial therapy is of clear benefit for patient's outcome. Here we propose a new protocol for species identification directly from positive blood cultures. EXPERIMENTAL DESIGN: We compared four in-house protocols for bacterial identification by mass spectrometry directly from clinical positive blood cultures evaluating 2 lytic agents, SDS and saponin, and 2 protein extraction schemes, fast (FP) and long (LP)...
December 6, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27919649/maldi-tof-ms-identification-of-species-from-the-acinetobacter-baumannii-ab-group-revisited-inclusion-of-the-novel-a-seifertii-and-a-dijkshoorniae-species
#2
Marta Marí-Almirall, Clara Cosgaya, Paul G Higgins, Ado Van Assche, Murat Telli, Geert Huys, Bart Lievens, Harald Seifert, Lenie Dijkshoorn, Ignasi Roca, Jordi Vila
OBJECTIVES: Rapid identification of Acinetobacter species is critical since members of the A. baumannii (Ab) group differ in antibiotic susceptibility and clinical outcomes. A. baumannii, A. pittii and A. nosocomialis can be identified by MALDI-TOF/MS, while the novel species A. seifertii and A. dijkshoorniae cannot. Low identification rates for A. nosocomialis have also been reported. We evaluated the use of MALDI-TOF/MS to identify isolates of A. seifertii and A. dijkshoorniae and revisited the identification of A...
December 2, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27918491/clarification-of-the-antagonistic-effect-of-the-lipopeptides-produced-by-bacillus-amyloliquefaciens-bpd1-against-pyricularia-oryzae-via-in-situ-maldi-tof-ims-analysis
#3
Jen-Hung Liao, Pi-Yu Chen, Yu-Liang Yang, Shu-Chen Kan, Feng-Chia Hsieh, Yung-Chang Liu
This study tried to clarify the antagonistic effect of the lipopeptides secreted by Bacillus amyloliquefaciens strain BPD1 (Ba-BPD1) against Pyricularia oryzae Cavara (PO). To determine the major antifungal lipopeptides effective against PO, single and dual cultures were carried out in solid-state media. The matrix-assisted laser desorption/ionization-time of flight imaging mass spectrometry (MALDI-TOF IMS) was used to identify the most effective lipopeptide in situ. Meanwhile, the morphology of pathogen fungi treated with lipopeptides was observed via the SEM...
December 3, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27916604/carbapenem-resistant-and-cephalosporin-susceptible-a-worrisome-phenotype-among-pseudomonas-aeruginosa-clinical-isolates-in-brazil
#4
Eloiza Helena Campana, Danilo Elias Xavier, Fernanda Villas-Boas Petrolini, Jhonatha Rodrigo Cordeiro-Moura, Maria Rita Elmor de Araujo, Ana Cristina Gales
The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility (Carb-R/Ceph-S), were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-Lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays...
December 1, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27914013/inhibitory-effect-of-homocysteine-on-rat-neural-stem-cell-growth-in-vitro-is-associated-with-reduced-protein-levels-and-enzymatic-activities-of-aconitase-and-respiratory-complex-iii
#5
Xu-Mei Zhang, Ya-Qian Zhao, Hai Yan, Huan Liu, Guo-Wei Huang
Increased blood plasma concentration of the sulphur amino acid homocysteine (Hcy) is considered as an independent risk factor of the neurodegenerative diseases. However, the detailed molecular mechanisms by which Hcy leads to neurotoxicity have yet to be clarified. Recent research has suggested that neurotoxicity of Hcy may involve negative regulation of neural stem cell (NSC) proliferation. In the current study, primary NSCs were isolated from neonatal rat brain hippocampus and the inhibition in cell growth was observed after exposure to l50 μM and 500 μM L-Hcy...
December 2, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27914002/phenotypic-and-genotypic-characterization-of-bacteriocinogenic-enterococci-against-clostridium-botulinum
#6
Awad A Shehata, Reda Tarabees, Shereen Basiouni, Mahmoud Gamil, Ahmed S Kamal, Monika Krüger
The present study aimed to characterize Enterococcus faecalis (n = -6) and Enterococcus faecium (n = 1) isolated from healthy chickens to find a novel perspective probiotic candidate that antagonize Clostridium botulinum types A, B, D, and E. The isolated enterococci were characterized based on phenotypic properties, PCR, and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF). The virulence determinants including hemolytic activity on blood agar, gelatinase activity, sensitivity to vancomycin, and presence of gelatinase (gelE) and enterococcal surface protein (esp) virulence genes were investigated...
December 2, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27913076/biofilm-production-and-beta-lactamic-resistance-in-brazilian-staphylococcus-aureus-isolates-from-bovine-mastitis
#7
Viviane Figueira Marques, Cássia Couto da Motta, Bianca da Silva Soares, Dayanne Araújo de Melo, Shana de Mattos de Oliveira Coelho, Irene da Silva Coelho, Helene Santos Barbosa, Miliane Moreira Soares de Souza
Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation...
October 18, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27905002/disseminated-candidiasis-in-a-young-previously-healthy-dog-and-review-of-literature
#8
Nicole Willems, Dirk J Houwers, Yvette M Schlotter, Bart Theelen, Teun Boekhout
BACKGROUND: The reports on disseminated candidiasis in dogs so far describe at least one predisposing factor. This case report, however, highlights candidiasis in a dog without any known predisposition. PATIENT: A 1.5-year-old intact female Hovawart dog was presented with subcutaneous nodules and polyuria/polydipsia. An excisional biopsy revealed a chronic pyogranulomatous and necrotizing inflammation with mycotic structures. The patient became febrile and lethargic, and developed lameness...
November 30, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27904505/composition-and-biological-activities-of-the-aqueous-extracts-of-three-scleractinian-corals-from-the-mexican-caribbean-pseudodiploria-strigosa-porites-astreoides-and-siderastrea-siderea
#9
Alejandro García-Arredondo, Alejandra Rojas-Molina, César Ibarra-Alvarado, Fernando Lazcano-Pérez, Roberto Arreguín-Espinosa, Judith Sánchez-Rodríguez
BACKGROUND: Scleractinian corals (stony corals) are the most abundant reef-forming cnidarians found in coral reefs throughout the world. Despite their abundance and ecological importance, information about the diversity of their toxins and their biological activities is very scarce. In this study, the chemical composition and the biological activities of the aqueous extracts of Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea, three scleractinian corals from the Mexican Caribbean, have been assessed for the first time...
2016: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/27902301/isolation-of-vibrio-tapetis-from-two-native-fish-species-genypterus-chilensis-and-paralichthys-adspersus-reared-in-chile-and-description-of-vibrio-tapetis-subsp-quintayensis-subsp-nov
#10
Arturo Levican, Aide Lasa, Rute Irgang, Jesús L Romalde, Matías Poblete-Morales, Ruben Avendaño-Herrera
Seven isolates recovered from diseased fine flounders (Paralichthys adspersus) and red conger eel (Genypterus chilensis) experimentally reared in Quintay (Chile) were confirmed as V. tapetis on the basis of the MALDI-TOF MS, 16S rRNA gene, DNA-DNA hybridization values and G+C content. The ERIC-PCR and REP-PCR patterns were homogeneous among those isolates recovered from a same host, but distinct to the type strains V. tapetis subsp. tapetis CECT 4600T and V. tapetis subsp. britannicus CECT 8161T. On the basis of atpA, rpoA, rpoD, recA and pyrH gene similarities (99...
November 30, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902286/macrococcus-canis-sp-nov-a-skin-bacterium-associated-with-infections-in-dogs
#11
Stefanie Gobeli Brawand, Kerstin Cotting, Elena Gómez-Sanz, Alexandra Collaud, Andreas Thomann, Isabelle Brodard, Sabrina Rodriguez Campos, Christian Strauss, Vincent Perreten
Gram-stain positive cocci were isolated from miscellaneous sites of the skin of healthy dogs as well as from infection sites in dogs. The closest relative by sequencing of the 16S rRNA was Macrococcus caseolyticus with 99.7% sequence identity, but compared to M. caseolyticus, the novel strains shared only 90.8% to 93.5% DNA identity to cpn60, dnaJ, rpoB, and sodA partial genes, respectively. The two species also exhibited other phenotypic characteristics and the majority displayed a visible haemolysis on sheep blood agar, while M...
November 16, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902273/racemicystis-persica-sp-nov-a-novel-myxobacterium-from-iranian-soil
#12
Joachim Wink, Azam Moradi, Gholam Hosein Ebrahimipour, Kathrin Mohr, Peter Kämpfer, Stefanie Glaeser, Fabienne Hennessen, Katja Gemperlein, Awal Prasad Ram, Corinna Wolf, Rolf Müller
A novel myxobacterium, strain MSr11462T, was isolated in 2015 from a soil sample collected from Kish Island beach, Persian Gulf, Iran. It displayed general myxobacterial features like Gram negative staining, rod shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting body like aggregates and myxospore like structures. The strain was mesophilic, aerobic and showed chemoheterotrophic mode of nutrition. It was resistant to many antibiotics like gentamycin, polymyxin, Fusidic acid and trimethopril and the key fatty acids of whole cell hydrolysates were iso-C15:0, C16:0, iso-C17:0, C18:1 , iso-C17:1 2-OH, C18:1 2-OH, iso-C15:0 OAG (O-alkylglycerol), and C16:1 OAG...
November 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902242/rapid-and-accurate-identification-of-pediococcus-species-isolated-from-korean-fermented-foods-by-maldi-tof-ms-with-local-database-extension
#13
Youngjae Cho, Eiseul Kim, Yoonju Lee, Sun-Kyung Han, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Pediococci are halophilic lactic acid bacteria, within the family Lactobacillaceae, which are involved in the fermentation of various salted and fermented foods, such as kimchi and jeotgal. In this study, a MALDI-TOF MS method was developed for the rapid identification of Pediococcus species. Of the 130 Pediococcus spectra aligned with the Biotyper taxonomy database, 122 isolates (93.9%) yielded log scores <1.7, which means they were not identifiable. After registering the spectra of 11 Pediococcus reference strains, all of the isolates were correctly identified, of which 84 (64...
October 27, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27896481/high-perineal-and-overall-frequency-of-staphylococcus-aureus-in-people-who-inject-drugs-compared-to-non-injectors
#14
Disa Dahlman, Farnaz Jalalvand, Marianne Alanko Blomé, Anders Håkansson, Håkan Janson, Susanne Quick, Anna C Nilsson
To investigate the prevalence, distribution, and colonization burden of Staphylococcus aureus (S. aureus) and MRSA in different body sites among people who inject drugs (PWID) and compare it to a control group consisting of non-injectors. In this cross-sectional survey, 49 active PWID from the needle exchange program (NEP) in Malmö, Sweden, and 60 non-injecting controls from an emergency psychiatric inpatient ward at Malmö Addiction Centre were tested for S. aureus (including MRSA) by culture, PCR, and MALDI-TOF...
November 28, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27895634/cytosolic-proteome-profiling-of-aminoglycosides-resistant-mycobacterium-tuberculosis-clinical-isolates-using-maldi-tof-ms
#15
Divakar Sharma, Manju Lata, Rananjay Singh, Nirmala Deo, Krishnamurthy Venkatesan, Deepa Bisht
Emergence of extensively drug resistant tuberculosis (XDR-TB) is the consequence of the failure of second line TB treatment. Aminoglycosides are the important second line anti-TB drugs used to treat the multi drug resistant tuberculosis (MDR-TB). Main known mechanism of action of aminoglycosides is to inhibit the protein synthesis by inhibiting the normal functioning of ribosome. Primary target of aminoglycosides are the ribosomal RNA and its associated proteins. Various mechanisms have been proposed for aminoglycosides resistance but still some are unsolved...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27895543/proteome-analysis-of-excretory-secretory-proteins-of-entamoeba-histolytica-hm1-imss-via-lc-esi-ms-ms-and-lc-maldi-tof-tof
#16
Jorim Anak Ujang, Soon Hong Kwan, Mohd Nazri Ismail, Boon Huat Lim, Rahmah Noordin, Nurulhasanah Othman
BACKGROUND: Excretory-secretory (ES) proteins of E. histolytica are thought to play important roles in the host invasion, metabolism, and defence. Elucidation of the types and functions of E. histolytica ES proteins can further our understanding of the disease pathogenesis. Thus, the aim of this study is to use proteomics approach to better understand the complex ES proteins of the protozoa. METHODS: E. histolytica ES proteins were prepared by culturing the trophozoites in protein-free medium...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27895315/identification-of-aberrantly-expressed-glycans-in-gastric-cancer-by-integrated-lectin-microarray-and-mass-spectrometric-analyses
#17
Xiang Li, Feng Guan, Dongliang Li, Zengqi Tan, Ganglong Yang, Yanli Wu, Zhaohui Huang
Cancer progression is usually associated with alterations of glycan expression patterns. Little is known regarding global glycomics in gastric cancer, the most common type of epithelial cancer. We integrated lectin microarray and mass spectrometry (MS) methods to profile glycan expression in three gastric cancer cell lines (SGC-7901, HGC-27, and MGC-803) and one normal gastric epithelial cell line (GES-1). Significantly altered glycans were confirmed by lectin staining and MALDI-TOF/TOF-MS. The three cancer cell lines showed increased levels of core-fucosylated N-glycans, GalNAcα-Ser/Thr (Tn antigen), and Sia2-6Galβ1-4GlcNAc N-glycans, but reduced levels of biantennary N-glycans, Galβ1-3GalNAcα-Ser/Thr (T antigen), and (GlcNAc)n N-glycans...
November 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27891772/twoplex-12-13-c6-aniline-stable-isotope-and-linkage-specific-sialic-acid-labelling-2d-lc-ms-workflow-for-quantitative-n-glycomics
#18
Simone Albrecht, Stefan Mittermayr, Josh Smith, Silvia Millán Martín, Margaret Doherty, Jonathan Bones
Quantitative glycomics represents an actively expanding research field ranging from the discovery of disease associated glycan alterations to the quantitative characterization of N-glycans on therapeutic proteins. Commonly used analytical platforms for comparative relative quantitation of complex glycan samples include MALDI-ToF-MS or chromatographic glycan profiling with subsequent data alignment and statistical evaluation. Limitations of such approaches include run-to-run technical variation and the potential introduction of subjectivity during data processing...
November 28, 2016: Proteomics
https://www.readbyqxmd.com/read/27891120/two-major-clades-of-bradyrhizobia-dominate-symbiotic-interactions-with-pigeonpea-in-fields-of-c%C3%A3-te-d-ivoire
#19
Romain K Fossou, Dominik Ziegler, Adolphe Zézé, François Barja, Xavier Perret
In smallholder farms of Côte d'Ivoire, particularly in the northeast of the country, Cajanus cajan (pigeonpea) has become an important crop because of its multiple beneficial facets. Pigeonpea seeds provide food to make ends meet, are sold on local markets, and aerial parts serve as forage for animals. Since it fixes atmospheric nitrogen in symbiosis with soil bacteria collectively known as rhizobia, C. cajan also improves soil fertility and reduces fallow time. Yet, seed yields remain low mostly because farmers cannot afford chemical fertilizers...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27891064/proteomic-based-approach-for-characterizing-4-hydroxy-2-nonenal-induced-oxidation-of-buffalo-bubalus-bubalis-and-goat-capra-hircus-meat-myoglobins
#20
Naveena B Maheswarappa, K Usha Rani, Y Praveen Kumar, Vinayak V Kulkarni, Srikanth Rapole
BACKGROUND: Myoglobin (Mb) is a sarcoplasmic heme protein primarily responsible for meat color and its chemistry is species specific. 4-hydroxy-2-nonenal (HNE) is a cytotoxic lipid derived aldehyde detected in meat and was reported to covalently adduct with nucleophilic histidine residues of Mb and predispose it to greater oxidation. However, no literature is available on characterization of lipid oxidation induced oxidation of Indian water buffalo (Bubalus bubalis) and goat (Capra hircus) myoglobins...
2016: Proteome Science
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