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James gibson

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https://www.readbyqxmd.com/read/28815907/t7-rna-polymerase-driven-inducible-cell-lysis-for-dna-transfer-from-escherichia-coli-to-bacillus-subtilis
#1
Mario Juhas, James W Ajioka
The majority of the good DNA editing techniques have been developed in Escherichia coli; however, Bacillus subtilis is better host for a plethora of synthetic biology and biotechnology applications. Reliable and efficient systems for the transfer of synthetic DNA between E. coli and B. subtilis are therefore of the highest importance. Using synthetic biology approaches, such as streamlined lambda Red recombineering and Gibson Isothermal Assembly, we integrated genetic circuits pT7L123, Repr-ts-1 and pLT7pol encoding the lysis genes of bacteriophages MS2, ΦX174 and lambda, the thermosensitive repressor and the T7 RNA polymerase into the E...
August 16, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28814411/local-synthesis-of-sex-hormones-are-there-consequences-for-the-ocular-surface-and-dry-eye
#2
REVIEW
Emma J Gibson, Fiona Stapleton, James S Wolffsohn, Blanka Golebiowski
Sex hormones are associated with the physiology and pathophysiology of almost all organs in the body, as well as most diseases. Interest in the associations between sex hormones and ocular tissues has increased in recent years. Androgens may have a positive effect on dry eye, whereas the effects of oestrogen on ocular conditions remain unclear. Intracrinology, the local synthesis and metabolism of hormones that is unique to humans, is of relevance to the eye and may help to explain why studies of the relationship between oestrogens and dry eye signs and symptoms are inconclusive...
August 16, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28805827/transcriptional-risk-scores-link-gwas-to-eqtls-and-predict-complications-in-crohn-s-disease
#3
Urko M Marigorta, Lee A Denson, Jeffrey S Hyams, Kajari Mondal, Jarod Prince, Thomas D Walters, Anne Griffiths, Joshua D Noe, Wallace V Crandall, Joel R Rosh, David R Mack, Richard Kellermayer, Melvin B Heyman, Susan S Baker, Michael C Stephens, Robert N Baldassano, James F Markowitz, Mi-Ok Kim, Marla C Dubinsky, Judy Cho, Bruce J Aronow, Subra Kugathasan, Greg Gibson
Gene expression profiling can be used to uncover the mechanisms by which loci identified through genome-wide association studies (GWAS) contribute to pathology. Given that most GWAS hits are in putative regulatory regions and transcript abundance is physiologically closer to the phenotype of interest, we hypothesized that summation of risk-allele-associated gene expression, namely a transcriptional risk score (TRS), should provide accurate estimates of disease risk. We integrate summary-level GWAS and expression quantitative trait locus (eQTL) data with RNA-seq data from the RISK study, an inception cohort of pediatric Crohn's disease...
August 14, 2017: Nature Genetics
https://www.readbyqxmd.com/read/28800634/utilizing-the-r-v-marcus-g-langseth-s-streamer-to-measure-the-acoustic-radiation-of-its-seismic-source-in-the-shallow-waters-of-new-jersey-s-continental-shelf
#4
Timothy J Crone, Maya Tolstoy, James C Gibson, Gregory Mountain
Shallow water marine seismic surveys are necessary to understand a range of Earth processes in coastal environments, including those that represent major hazards to society such as earthquakes, tsunamis, and sea-level rise. Predicting the acoustic radiation of seismic sources in shallow water, which is required for compliance with regulations designed to limit impacts on protected marine species, is a significant challenge in this environment because of variable reflectivity due to local geology, and the susceptibility of relatively small bathymetric features to focus or shadow acoustic energy...
2017: PloS One
https://www.readbyqxmd.com/read/28789551/adsorption-and-charge-transfer-interactions-of-bi-isonicotinic-acid-on-ag-111
#5
Robert H Temperton, Andrew J Gibson, Karsten Handrup, James N O'Shea
The adsorption and charge transfer dynamics of the organic molecule bi-isonicotinic acid (4,4-dicarboxy-2,2-bipyridine) on single crystal Ag(111) has been studied using synchrotron radiation-based photoemission, x-ray absorption, and resonant core spectroscopies. Measurements for multilayer and monolayer coverage are used to determine the nature of the molecule-surface interactions and the molecular orientation. An experimental density of states for the monolayer with respect to the underlying metal surface is obtained by combining x-ray absorption spectroscopy at the N 1s edge and valence photoemission to measure the unoccupied and occupied valence states, respectively...
August 7, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28757455/affordances-and-neuroscience-steps-towards-a-successful-marriage
#6
REVIEW
Matthieu M de Wit, Simon de Vries, John van der Kamp, Rob Withagen
The concept of affordance is rapidly gaining popularity in neuroscientific accounts of perception and action. This concept was introduced by James Gibson to refer to the action possibilities of the environment. By contrast, standard cognitive neuroscience typically uses the concept to refer to (action-oriented) representations in the brain. This paper will show that the view of affordances as representations firmly places the concept in the subject-object framework that dominates both psychology and neuroscience...
July 27, 2017: Neuroscience and Biobehavioral Reviews
https://www.readbyqxmd.com/read/28747888/the-hsp72-and-hsp90%C3%AE-mrna-responses-to-hot-downhill-running-are-reduced-following-a-prior-bout-of-hot-downhill-running-and-occur-concurrently-within-leukocytes-and-the-vastus-lateralis
#7
James A Tuttle, Bryna C R Chrismas, Oliver R Gibson, James H Barrington, David C Hughes, Paul C Castle, Alan J Metcalfe, Adrian W Midgley, Oliver Pearce, Chindu Kabir, Faizal Rayanmarakar, Sami Al-Ali, Mark P Lewis, Lee Taylor
The leukocyte heat shock response (HSR) is used to determine individual's thermotolerance. The HSR and thermotolerance are enhanced following interventions such as preconditioning and/or acclimation/acclimatization. However, it is unclear whether the leukocyte HSR is an appropriate surrogate for the HSR in other tissues implicated within the pathophysiology of exertional heat illnesses (e.g., skeletal muscle), and whether an acute preconditioning strategy (e.g., downhill running) can improve subsequent thermotolerance...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28731643/transcriptome-based-identification-of-the-optimal-reference-cho-genes-for-normalisation-of-qpcr-data
#8
Adam J Brown, Suzanne Gibson, Diane Hatton, David C James
Real-time quantitative PCR (qPCR) is the standard method for determination of relative changes in mRNA transcript abundance. Analytical accuracy, precision, and reliability are critically dependent on the selection of internal control reference genes. In this study we have identified optimal reference genes that can be utilized universally for qPCR analysis of CHO cell mRNAs. Initially, transcriptomic datasets were analysed to identify eight endogenous genes that exhibited high expression stability across four distinct CHO cell lines sampled in different culture phases...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28714766/cancer-related-fatigue-in-adolescents-and-young-adults-after-cancer-treatment-persistent-and-poorly-managed
#9
Anna Spathis, Helen Hatcher, Sara Booth, Faith Gibson, Paddy Stone, Laura Abbas, Matt Barclay, James Brimicombe, Pia Thiemann, Martin G McCabe, Rachel Campsey, Louise Hooker, Wendy Moss, Jane Robson, Stephen Barclay
Cancer-related fatigue is the most prevalent and distressing symptom experienced by adolescents and young adults (AYAs). An electronic survey was undertaken to ascertain current fatigue management and perceptions of its effectiveness. Eighty-five percent of responders (68/80) experienced fatigue, and it was worse more than 1 year after cancer treatment ended, compared to <1 year (p = 0.007). Forty-one percent received no fatigue management. Although advice to exercise was the most frequent intervention, the greatest impact of fatigue was on the ability to exercise and most did not find exercise advice helpful...
July 17, 2017: Journal of Adolescent and Young Adult Oncology
https://www.readbyqxmd.com/read/28701973/schizophrenia-an-impairment-in-the-capacity-to-perceive-affordances
#10
Nam-Gyoon Kim, Hakboon Kim
Phenomenological psychopathologists conceptualize schizophrenia as a self-disorder involving profound distortions of selfhood. For James Gibson, "to perceive the world is to coperceive oneself." If the sense of self is disturbed in individuals with schizophrenia, this could also lead to disturbances in these individuals' ability to perceive affordances, environmental properties taken with reference to the perceiver's action capabilities (e.g., a rigid surface affording 'walk-on-able,' chairs 'sit-on-able,' and so on)...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28687413/effect-of-azithromycin-on-asthma-exacerbations-and-quality-of-life-in-adults-with-persistent-uncontrolled-asthma-amazes-a-randomised-double-blind-placebo-controlled-trial
#11
Peter G Gibson, Ian A Yang, John W Upham, Paul N Reynolds, Sandra Hodge, Alan L James, Christine Jenkins, Matthew J Peters, Guy B Marks, Melissa Baraket, Heather Powell, Steven L Taylor, Lex E X Leong, Geraint B Rogers, Jodie L Simpson
BACKGROUND: Exacerbations of asthma cause a substantial global illness burden. Adults with uncontrolled persistent asthma despite maintenance treatment require additional therapy. Since macrolide antibiotics can be used to treat persistent asthma, we aimed to assess the efficacy and safety of oral azithromycin as add-on therapy in patients with uncontrolled persistent asthma on medium-to-high dose inhaled corticosteroids plus a long-acting bronchodilator. METHODS: We did a randomised, double-blind, placebo controlled parallel group trial to determine whether oral azithromycin decreases the frequency of asthma exacerbations in adults (≥18 years) with symptomatic asthma despite current use of inhaled corticosteroid and long-acting bronchodilator, and who had no hearing impairment or abnormal prolongation of the corrected QT interval...
July 4, 2017: Lancet
https://www.readbyqxmd.com/read/28662661/non-model-model-organisms
#12
EDITORIAL
James J Russell, Julie A Theriot, Pranidhi Sood, Wallace F Marshall, Laura F Landweber, Lillian Fritz-Laylin, Jessica K Polka, Snezhana Oliferenko, Therese Gerbich, Amy Gladfelter, James Umen, Magdalena Bezanilla, Madeline A Lancaster, Shuonan He, Matthew C Gibson, Bob Goldstein, Elly M Tanaka, Chi-Kuo Hu, Anne Brunet
Model organisms are widely used in research as accessible and convenient systems to study a particular area or question in biology. Traditionally only a handful of organisms have been widely studied, but modern research tools are enabling researchers to extend the set of model organisms to include less-studied and more unusual systems. This Forum highlights a range of 'non-model model organisms' as emerging systems for tackling questions across the whole spectrum of biology (and beyond), the opportunities and challenges, and the outlook for the future...
June 29, 2017: BMC Biology
https://www.readbyqxmd.com/read/28654986/association-between-initial-use-of-e-cigarettes-and-subsequent-cigarette-smoking-among-adolescents-and-young-adults-a-systematic-review-and-meta-analysis
#13
Samir Soneji, Jessica L Barrington-Trimis, Thomas A Wills, Adam M Leventhal, Jennifer B Unger, Laura A Gibson, JaeWon Yang, Brian A Primack, Judy A Andrews, Richard A Miech, Tory R Spindle, Danielle M Dick, Thomas Eissenberg, Robert C Hornik, Rui Dang, James D Sargent
Importance: The public health implications of e-cigarettes depend, in part, on whether e-cigarette use affects the risk of cigarette smoking. Objective: To perform a systematic review and meta-analysis of longitudinal studies that assessed initial use of e-cigarettes and subsequent cigarette smoking. Data Sources: PubMed, EMBASE, Cochrane Library, Web of Science, the 2016 Society for Research on Nicotine and Tobacco 22nd Annual Meeting abstracts, the 2016 Society of Behavioral Medicine 37th Annual Meeting & Scientific Sessions abstracts, and the 2016 National Institutes of Health Tobacco Regulatory Science Program Conference were searched between February 7 and February 17, 2017...
August 1, 2017: JAMA Pediatrics
https://www.readbyqxmd.com/read/28598549/the-presence-of-minocycline-in-the-tear-film-of-normal-horses-following-oral-administration-and-its-anticollagenase-activity
#14
Caroline S Monk, Sun Young Jeong, Daniel James Gibson, Caryn E Plummer
PURPOSE: Tetracyclines have activity against matrix metalloproteinases (MMP). Oral medications with effects on the ocular surface are of interest in patients where repeated topical dosing is limited. The aim of this study was to characterize the concentration of minocycline in the tears of normal horses after oral administration and to determine if this level directly inhibits MMP activity. METHODS: Five healthy adult ponies were administered oral minocycline (Wedgewood Pharmacy; Swedesboro, NJ) at 4 mg/kg every 12 h for 5 days...
June 9, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28575699/a-novel-spliced-variant-of-the-tin2-shelterin-is-present-in-chronic-lymphocytic-leukemia
#15
Ganchimeg Ishdorj, Sara E F Kost, Sara Beiggi, Yunli Zang, Spencer B Gibson, James B Johnston
The shelterin proteins play important roles in telomere maintenance and genome stability. These proteins have been found to be mutated in many cancers including CLL. Herein, we demonstrate here the presence of a novel spliced isoform of TIN2S in chronic lymphocytic leukemia (CLL), related to deletion of exon 2 in the TIN2 gene. The expressions of spliced TIN2S mRNA varied widely in CLL and there was an inverse relationship between the mRNA levels of full-length TIN2S and the spliced moiety. Small amounts of spliced TIN2S were also observed in normal B cells but not in T cells...
May 29, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28534645/the-mitochondria-targeting-peptide-elamipretide-diminishes-circulating-htra2-in-st-segment-elevation-myocardial-infarction
#16
Marcus Hortmann, Samuel Robinson, Moritz Mohr, Maximillian Mauler, Daniela Stallmann, Jochen Reinöhl, Daniel Duerschmied, Karlheinz Peter, James Carr, C Michael Gibson, Christoph Bode, Ingo Ahrens
BACKGROUND: The extent of myocardial damage in patients with ST-segment elevation myocardial infarction (STEMI) depends on both the time to reperfusion as well as injury induced by ischaemia-reperfusion resulting in a cascade of cellular and humoral reactions. As a consequence of ischaemia-reperfusion in the heart, the high-temperature requirement serine peptidase 2 (HtrA2) is translocated from the mitochondria to the cytosol, whereupon it induces protease activity-dependent apoptosis mediated via caspases...
May 1, 2017: European Heart Journal. Acute Cardiovascular Care
https://www.readbyqxmd.com/read/28507745/performance-measures-for-lower-gastrointestinal-endoscopy-a-european-society-of-gastrointestinal-endoscopy-esge-quality-improvement-initiative
#17
REVIEW
Michal F Kaminski, Siwan Thomas-Gibson, Marek Bugajski, Michael Bretthauer, Colin J Rees, Evelien Dekker, Geir Hoff, Rodrigo Jover, Stepan Suchanek, Monika Ferlitsch, John Anderson, Thomas Roesch, Rolf Hultcranz, Istvan Racz, Ernst J Kuipers, Kjetil Garborg, James E East, Maciej Rupinski, Birgitte Seip, Cathy Bennett, Carlo Senore, Silvia Minozzi, Raf Bisschops, Dirk Domagk, Roland Valori, Cristiano Spada, Cesare Hassan, Mario Dinis-Ribeiro, Matthew D Rutter
The European Society of Gastrointestinal Endoscopy and United European Gastroenterology present a short list of key performance measures for lower gastrointestinal endoscopy. We recommend that endoscopy services across Europe adopt the following seven key performance measures for lower gastrointestinal endoscopy for measurement and evaluation in daily practice at a center and endoscopist level: 1 rate of adequate bowel preparation (minimum standard 90%); 2 cecal intubation rate (minimum standard 90%); 3 adenoma detection rate (minimum standard 25%); 4 appropriate polypectomy technique (minimum standard 80%); 5 complication rate (minimum standard not set); 6 patient experience (minimum standard not set); 7 appropriate post-polypectomy surveillance recommendations (minimum standard not set)...
April 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28499033/large-scale-recoding-of-a-bacterial-genome-by-iterative-recombineering-of-synthetic-dna
#18
Yu Heng Lau, Finn Stirling, James Kuo, Michiel A P Karrenbelt, Yujia A Chan, Adam Riesselman, Connor A Horton, Elena Schäfer, David Lips, Matthew T Weinstock, Daniel G Gibson, Jeffrey C Way, Pamela A Silver
The ability to rewrite large stretches of genomic DNA enables the creation of new organisms with customized functions. However, few methods currently exist for accumulating such widespread genomic changes in a single organism. In this study, we demonstrate a rapid approach for rewriting bacterial genomes with modified synthetic DNA. We recode 200 kb of the Salmonella typhimurium LT2 genome through a process we term SIRCAS (stepwise integration of rolling circle amplified segments), towards constructing an attenuated and genetically isolated bacterial chassis...
May 12, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28486332/short-term-heat-acclimation-and-precooling-independently-and-combined-improve-5-km-running-performance-in-the-heat
#19
C A James, A J Richardson, A G B Willmott, P W Watt, O R Gibson, N S Maxwell
Following heat acclimation (HA), endurance running performance remains impaired in hot vs temperate conditions. Combining HA with precooling demonstrates no additive benefit in intermittent sprint, or continuous cycling exercise protocols, during which heat strain may be less severe compared to endurance running. This study investigated the effect of short-term heat acclimation (STHA) combined with mixed-methods precooling, on endurance running performance and directly compared precooling and HA. Nine amateur trained runners completed 5 km treadmill time trials in the heat (32°C, 60% RH) under four conditions; no intervention (CON), precooling (PC), short-term heat acclimation (5 days - HA) and short-term heat acclimation with precooling (HA+PC)...
May 5, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28479329/inflammatory-phenotypes-in-severe-asthma-are-associated-with-distinct-airway-microbiology
#20
Steven L Taylor, Lex E X Leong, Jocelyn M Choo, Steve Wesselingh, Ian A Yang, John W Upham, Paul N Reynolds, Sandra Hodge, Alan L James, Christine Jenkins, Matthew J Peters, Melissa Baraket, Guy B Marks, Peter G Gibson, Jodie L Simpson, Geraint B Rogers
BACKGROUND: Asthma pathophysiology and treatment responsiveness are predicted by inflammatory phenotype. However, the relationship between airway microbiology and asthma phenotype is poorly understood. We aimed to characterise airway microbiota in patients with symptomatic stable asthma, and relate composition to airway inflammatory phenotype and other phenotypic characteristics. METHODS: The microbial composition of induced sputum specimens collected from adult patients screened for a multicenter randomized controlled trial was determined by 16S rRNA gene sequencing...
May 4, 2017: Journal of Allergy and Clinical Immunology
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