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https://www.readbyqxmd.com/read/19623896/in-vitro-amplification-of-bvdv-field-strains-isolated-in-argentina-effect-of-cell-line-and-culture-conditions
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COMPARATIVE STUDY
A C Odeón, M R Leunda, C Faverín, N Boynak, M M Vena, O Zabal
The aim of this work was to study the in vitro amplification of BVDV (Pestivirus, Flaviridae) field isolates from Argentina in MDBK, BoTur and BHK-21 continuous cell lines. Field isolates 99/134 (mucosal disease), 00/693 (mucosal disease), 04P7016 (respiratory disease) and 04/89 (mucosal disease), genotype 1b, were used and compared with the Singer and NADL reference strains, genotype 1a. Additionally, cell lines derived from explants of bovine testis (RD-420), bovine uterus (NCL-1) and porcine kidney (PKZ) were tested as alternative substrates for BVDV propagation in vitro...
April 2009: Revista Argentina de Microbiología
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