keyword
MENU ▼
Read by QxMD icon Read
search

Cell culture

keyword
https://www.readbyqxmd.com/read/29236340/flow-cytometry-a-versatile-technology-for-specific-quantification-and-viability-assessment-of-microorganisms-in-multi-strain-probiotic-products
#1
Camille Chiron, Thomas A Tompkins, Pierre Burguière
AIMS: Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but non-culturable (VBNC) microorganisms and non-viable cells. The aim of the study was to evaluate flow cytometry as a novel method for the specific quantification of viable and non-viable probiotics in multi-strain products. METHODS AND RESULTS: Custom polyclonal antibodies were produced against five probiotic strains from different species (Bifidobacterium bifidum R0071, Bifidobacterium longum ssp...
December 13, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29236262/studying-nonsense-mediated-mrna-decay-in-mammalian-cells-using-a-multicolored-bioluminescence-based-reporter-system
#2
Andrew Nickless, Zhongsheng You
The nonsense-mediated mRNA decay (NMD) pathway degrades aberrant transcripts containing premature translation termination codons (PTCs) and also regulates the levels of many normal mRNAs containing NMD-inducing features. The activity of this pathway varies considerably in different cell types and can change in response to developmental and environmental cues. Modulating NMD activity represents a potential therapeutic avenue for certain genetic disorders and cancers. Simple reporter systems capable of faithfully assessing NMD activity in mammalian cells greatly facilitate both basic and translational research on NMD...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236248/determining-mrna-decay-rates-using-rna-approach-to-equilibrium-sequencing-rate-seq
#3
Farah Abdul-Rahman, David Gresham
RATE-seq is a 4-thiouracil (4-tU)-based method that enables the in vivo measurement of transcriptome-wide RNA degradation rates. 4-tU is an analog of uracil that is rapidly incorporated into newly synthesized RNA and facilitates the conjugation of a biotinylated molecule containing a reactive thiol group. The biotinylated RNA can then be fractionated from the unlabeled RNA with streptavidin magnetic beads. By adding 4-tU to a culture of cells growing in steady-state conditions, fractionating the labeled population of RNA at multiple time points following 4-tU addition, and quantifying the abundance of newly transcribed RNAs using RNAseq, it is possible to estimate the degradation rates of all transcripts in a single experiment...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29236192/inhibitory-effect-of-streptococci-on-the-growth-of-m-catarrhalis-strains-and-the-diversity-of-putative-bacteriocin-like-gene-loci-in-the-genomes-of-s-pneumoniae-and-its-relatives
#4
L N Ikryannikova, M V Malakhova, G G Lominadze, I Yu Karpova, E S Kostryukova, N A Mayansky, A N Kruglov, E A Klimova, E S Lisitsina, E N Ilina, V M Govorun
S. pneumoniae is a facultative human pathogen causing a wide range of infections including the life-threatening pneumoniae or meningitis. It colonizes nasopharynx as well as its closest phylogenetic relatives S. pseudopneumoniae and S. mitis. Both the latter, despite the considerable morphological and phenotypic similarity with the pneumococcus, are considerably less pathogenic for humans and cause infections mainly in the immunocompromized hosts. In this work, we compared the inhibitory effect of S. pneumoniae and its relatives on the growth of Moraxella catarrhalis strains using the culture-based antagonistic test...
December 13, 2017: AMB Express
https://www.readbyqxmd.com/read/29236151/the-effects-of-hap-and-macrophage-cells-to-the-expression-of-inflammatory-factors-and-apoptosis-in-hk-2-cells-of-vitro-co-cultured-system
#5
Junchuan Yu, Yaoliang Deng, Zhiwei Tao, Weixia Liang, Xiaofeng Guan, Jihua Wu, Xin Ning, Yunlong Liu, Quan Liu, Ziqi He
This study developed an in vitro system by co-culturing HK-2 cells with different concentration of hydroxyapatite (HAP) and/or macrophage cells to simulate the internal environment of urolithiasis as far as possible, investigating the regulatory effects of macrophage cells on HAP-induced expression of relative inflammatory factors of HK-2 cells. The control group (H group) was only comprised of HK-2 cells. Experimental groups included co-culturing HK-2 cells and macrophage cells (H + M group), co-culturing HK-2 cells and HAP (H + A group), co-culturing macrophage cells and HAP (M + A group), and co-culturing HK-2 cells and macrophage cells with HAP (H + M + A group)...
December 13, 2017: Urolithiasis
https://www.readbyqxmd.com/read/29236117/liquid-crystal-elastomer-foams-with-elastic-properties-specifically-engineered-as-biodegradable-brain-tissue-scaffolds
#6
M E Prévôt, H Andro, S L M Alexander, S Ustunel, C Zhu, Z Nikolov, S T Rafferty, M T Brannum, B Kinsel, L T J Korley, E J Freeman, J A McDonough, R J Clements, E Hegmann
Tissue regeneration requires 3-dimensional (3D) smart materials as scaffolds to promote transport of nutrients. To mimic mechanical properties of extracellular matrices, biocompatible polymers have been widely studied and a diverse range of 3D scaffolds have been produced. We propose the use of responsive polymeric materials to create dynamic substrates for cell culture, which goes beyond designing only a physical static 3D scaffold. Here, we demonstrated that lactone- and lactide-based star block-copolymers (SBCs), where a liquid crystal (LC) moiety has been attached as a side-group, can be crosslinked to obtain Liquid Crystal Elastomers (LCEs) with a porous architecture using a salt-leaching method to promote cell infiltration...
December 13, 2017: Soft Matter
https://www.readbyqxmd.com/read/29236055/hyaluronan-production-by-renomedullary-interstitial-cells-influence-of-endothelin-angiotensin-ii-and-vasopressin
#7
Sara Stridh, Fredrik Palm, Tomoko Takahashi, Mayumi Ikegami-Kawai, Malou Friederich-Persson, Peter Hansell
The content of hyaluronan (HA) in the interstitium of the renal medulla changes in relation to body hydration status. We investigated if hormones of central importance for body fluid homeostasis affect HA production by renomedullary interstitial cells in culture (RMICs). Simultaneous treatment with vasopressin and angiotensin II (Ang II) reduced HA by 69%. No change occurred in the mRNA expressions of hyaluronan synthase 2 (HAS2) or hyaluronidases (Hyals), while Hyal activity in the supernatant increased by 67% and CD44 expression reduced by 42%...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29236033/mast-cells-interact-with-endothelial-cells-to-accelerate-in-vitro-angiogenesis
#8
Devandir Antonio de Souza Junior, Vivian Marino Mazucato, Ana Carolina Santana, Constance Oliver, Maria Celia Jamur
Angiogenesis is a complex process that involves interactions between endothelial cells and various other cell types as well as the tissue microenvironment. Several previous studies have demonstrated that mast cells accumulate at angiogenic sites. In spite of the evidence suggesting a relationship between mast cells and angiogenesis, the association of mast cells and endothelial cells remains poorly understood. The present study aims to investigate the relationship between mast cells and endothelial cells during in vitro angiogenesis...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29236027/simvastatin-inhibits-cell-proliferation-and-migration-in-human-anaplastic-thyroid-cancer
#9
Mei-Chieh Chen, Yuan-Chin Tsai, Jen-Ho Tseng, Jr-Jiun Liou, Steve Horng, Heng-Ching Wen, Yu-Ching Fan, Wen-Bin Zhong, Sung-Po Hsu
Malignant human anaplastic thyroid cancer (ATC) is pertinacious to conventional therapies. The present study investigated the anti-cancer activity of simvastatin and its underlying regulatory mechanism in cultured ATC cells. Simvastatin (0-20 μM) concentration-dependently reduced cell viability and relative colony formation. Depletions of mevalonate (MEV) and geranylgeranyl pyrophosphate (GGpp) by simvastatin induced G1 arrest and increased apoptotic cell populations at the sub-G1 phase. Adding MEV and GGpp prevented the simvastatin-inhibited cell proliferation...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235977/proprotein-convertase-subtilisin-kexin-type-9-inhibits-hepatitis-c-virus-replication-through-interacting-with-ns5a
#10
Zhubing Li, Qiang Liu
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease actively involved in regulating lipid homeostasis. Although PCSK9 has been shown to inhibit hepatitis C virus (HCV) entry and replication, the underlying mechanisms have not been thoroughly characterized. Moreover, whether PCSK9 regulates HCV translation and assembly/secretion has not been determined. We therefore further studied the effects of PCSK9 on the HCV life cycle. We showed that PCSK9 did not affect HCV translation or assembly/secretion...
December 13, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29235973/linear-array-of-multi-substrate-tracts-for-simultaneous-assessment-of-cell-adhesion-migration-and-differentiation
#11
Ricardo A Moreno-Rodriguez, Edward L Krug, Leticia Reyes, Roger R Markwald
Cell migration, which is central to a wide variety of life processes, involves integration of the extracellular matrix (ECM) with the internal cytoskeleton and motor proteins via receptors spanning the plasma membrane. Cell migration can be induced by a variety of signals, including gradients of external soluble molecules, differences in ECM composition, or electrical gradients. Current in vitro methods to study cell migration only test one substrate at a time. Here, we present a method for assessing cell adhesion, migration, and differentiation in up to 20 different test conditions simultaneously, using only minute amounts of target substrate...
December 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/29235848/dual-data-independent-acquisition-approach-combining-global-hcp-profiling-and-absolute-quantification-of-key-impurities-during-bioprocess-development
#12
Gauthier Husson, Aurélie Delangle, John O'Hara, Sarah Cianférani, Annick Gervais, Alain Van Dorsselaer, Daniel G Bracewell, Christine Carapito
Host cell proteins (HCP) are a major class of impurities derived from recombinant protein production process. While HCP are usually monitored by ELISA, mass spectrometry (MS) based approaches are emerging as promising orthogonal methods. Here, we developed an original method relying on data independent acquisition (DIA) coupling global HCP amounts estimation (Top 3) and absolute quantification with isotope dilution (ID). The method named Top 3-ID-DIA was benchmarked against ELISA and a gold standard selected reaction monitoring assay (ID-SRM)...
December 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29235729/conductive-tough-hydrogel-for-bioapplications
#13
Mohammad Javadi, Qi Gu, Sina Naficy, Syamak Farajikhah, Jeremy M Crook, Gordon G Wallace, Stephen Beirne, Simon E Moulton
Biocompatible conductive tough hydrogels represent a new class of advanced materials combining the properties of tough hydrogels and biocompatible conductors. Here, a simple method, to achieve a self-assembled tough elastomeric composite structure that is biocompatible, conductive, and with high flexibility, is reported. The hydrogel comprises polyether-based liner polyurethane (PU), poly(3,4-ethylenedioxythiophene) (PEDOT) doped with poly(4-styrenesulfonate) (PSS), and liquid crystal graphene oxide (LCGO)...
December 13, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29235551/interleukin-1%C3%AE-induces-human-cementoblasts-to-support-osteoclastogenesis
#14
Nam C-N Huynh, Vincent Everts, Prasit Pavasant, Ruchanee S Ampornaramveth
Injury of the periodontium followed by inflammatory response often leads to root resorption. Resorption is accomplished by osteoclasts and their generation may depend on an interaction with the cells in direct contact with the root, the cementoblasts. Our study aimed to investigate the role of human cementoblasts in the formation of osteoclasts and the effect of interleukin (IL)-1β hereupon. Extracted teeth from healthy volunteers were subjected to sequential digestion by type I collagenase and trypsin. The effect of enzymatic digestion on the presence of cells on the root surface was analyzed by histology...
December 13, 2017: International Journal of Oral Science
https://www.readbyqxmd.com/read/29235536/genetically-unmatched-human-ipsc-and-esc-exhibit-equivalent-gene-expression-and-neuronal-differentiation-potential
#15
Hany E Marei, A Althani, S Lashen, C Cenciarelli, Anwarul Hasan
The potential uniformity between differentiation and therapeutic potential of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) remains debatable. We studied the gene expression profiles, pathways analysis and the ability to differentiated into neural progenitor cells (NPCs) and motor neurons (MNs) of genetically unmatched integration-free hiPSC versus hESC to highlight possible differences/similarities between them at the molecular level. We also provided the functional information of the neurons derived from the different hESCs and hiPSCs lines using the Neural Muscular Junction (NMJ) Assay...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29235204/plasma-activated-medium-pam-kills-human-cancer-initiating-cells
#16
Jun-Ichiro Ikeda, Hiromasa Tanaka, Kenji Ishikawa, Hajime Sakakita, Yuzuru Ikehara, Masaru Hori
Medical non-thermal plasma (NTP) treatments for various types of cancers have been reported. Cells with tumorigenic potential (cancer-initiating cells; CICs) are few in number in many types of tumors. CICs efficiently eliminate anti-cancer chemicals and exhibit high-level aldehyde dehydrogenase (ALDH) activity. We previously examined the effects of direct irradiation via NTP on cancer cells; even though we targeted CICs expressing high levels of ALDH, such treatment affected both non-CICs and CICs. Recent studies have shown that plasma-activated medium (PAM) (culture medium irradiated by NTP) selectively induces apoptotic death of cancer but not normal cells...
December 13, 2017: Pathology International
https://www.readbyqxmd.com/read/29235130/melt-electrospun-bilayered-scaffolds-for-tissue-integration-of-a-suture-less-inflow-cannula-for-rotary-blood-pumps
#17
Sam Liao, Christina Theodoropoulos, Keith A Blackwood, Maria A Woodruff, Shaun D Gregory
Implantation of left ventricular assist devices typically requires cardiopulmonary bypass support, which is associated with postoperative complications. A novel suture-less inflow cannula, which can be implanted without bypass, uses mild myocardial compression to seal the interface, however, this may lead to necrosis of the myocardium. To circumvent this issue, a bilayered scaffold has been developed to promote tissue growth at the interface between cannula and myocardium. The bilayered scaffold consists of a silicone base layer, which mimics the seal, and a melt electrospun polycaprolactone scaffold to serve as a tissue integration layer...
December 13, 2017: Artificial Organs
https://www.readbyqxmd.com/read/29235098/hepcidin-and-dna-promoter-methylation-in-hepatocellular-carcinoma
#18
Silvia Udali, Annalisa Castagna, Michela Corbella, Andrea Ruzzenente, Sara Moruzzi, Filippo Mazzi, Tommaso Campagnaro, Domenica De Santis, Antonia Franceschi, Patrizia Pattini, Rossella Gottardo, Oliviero Olivieri, Luigi Perbellini, Alfredo Guglielmi, Sang-Woon Choi, Domenico Girelli, Simonetta Friso
BACKGROUND: The liver hormone hepcidin regulates iron homeostasis that is often altered in hepatocellular carcinoma (HCC). Epigenetic phenomena control gene expression through a dynamic fashion, therefore, considering the plasticity of both iron homeostasis and epigenetic mechanisms and their role in liver carcinogenesis, we investigated whether hepcidin gene (HAMP) expression is modulated by DNA methylation thus affecting iron status in human HCC. MATERIALS AND METHODS: Thirty-two patients affected by non-viral HCC were enrolled and their main clinical and biochemical characteristics obtained...
December 13, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29235061/differential-expression-of-immune-factor-between-patients-with-chronic-prostatitis-chronic-pelvic-pain-syndrome-and-the-healthy-volunteers
#19
Chen Ye, Guang'an Xiao, Jian Xu, Shengfei Qin, Yuhua Luo, Guanghua Chen, H Henry Lai, Tie Zhou
PURPOSE: Immune mechanisms have been hypothesized to contribute to the development of CP/CPPS. In this study, we investigated the differential expression of immune factors between patients with CP/CPPS and healthy volunteers. METHODS: This study was registered in Australian New Zealand Clinical Trials Registry. Healthy volunteers and patients with CP/CPPS were enrolled in this study. The inclusion criteria for patients were below: (1) aged 18-45 years old; (2) prostatitis-related syndrome longer than 3 months; (3) normal routine urine culture and negative bacterial culture in prostatic fluid...
December 12, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/29235057/design-and-assessment-of-a-dynamic-perfusion-bioreactor-for-large-bone-tissue-engineering-scaffolds
#20
Birru Bhaskar, Robert Owen, Hossein Bahmaee, Parcha Sreenivasa Rao, Gwendolen C Reilly
Bioreactors can be used to apply fluid flow in vitro to scaffolds to improve mass transport of media and apply mechanical forces to cells. In this study, we developed and tested an autoclavable, modular perfusion bioreactor suitable for large scaffolds. We investigated the effects of fluid flow induced shear stress (FFSS) on osteogenic differentiation of human embryonic stem cell-derived mesenchymal progenitors (hES-MP cells) cultured on large polyurethane (PU) scaffolds (30 mm diameter × 5 mm thickness) in osteogenesis induction media (OIM)...
December 12, 2017: Applied Biochemistry and Biotechnology
keyword
keyword
52577
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"