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https://www.readbyqxmd.com/read/28651420/-effects-of-hypoxia-inducible-factor-1%C3%AE-on-p311-and-its-influence-on-the-migration-of-murine-epidermal-stem-cells
#1
Z D Xu, H S Li, S Wang, W F He, J Wu, G X Luo
Objective: To explore the effects of hypoxia inducible factor-1α (HIF-1α) on P311 and its influence on the migration of murine epidermal stem cells (ESCs) under hypoxia in vitro. Methods: Two kinds of murine ESCs were isolated and obtained from 15 neonatal wild-type C57BL/6J mice and 5 congeneric source P311 gene knock-out mice, respectively. The first passage of cells were used in the following experiments after morphologic observation and detection of expression of cell surface markers CD71 and CD49f with flow cytometer...
May 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28651331/il-1%C3%AE-up-regulates-star-and-progesterone-production-through-the-erk1-2-and-p38-mediated-creb-signaling-pathways-in-human-granulosa-lutein-cells
#2
Xuan Dang, Qinling Zhu, Yaqiong He, Yuan Wang, Yao Lu, Xiaoxue Li, Jia Qi, Hasiximuke Wu, Yun Sun
The pro-inflammatory cytokine interleukin-1β (IL-1β) may be involved in several ovulation-associated events, such as proteases synthesis, prostaglandin production and steroidogenesis in granulosa cells. However, the exact effect of IL-1β on progesterone synthesis in granulosa cells and the underlying mechanism remain unclear. By using cultured granulosa-lutein cells collected from women undergoing in vitro fertilization or intracytoplasmic sperm injection, we found that IL-1β up-regulated steroidogenic acute regulatory protein (StAR) expression and progesterone synthesis in granulosa-lutein cells, which was comparable with luteinizing hormone (LH) effect and could be abolished by an IL-1 receptor antagonist...
June 23, 2017: Endocrinology
https://www.readbyqxmd.com/read/28651246/development-of-a-perfusion-platform-for-dynamic-cultivation-of-in-vitro-skin-models
#3
Kay Strüver, Wolfgang Friess, Sarah Hedtrich
Reconstructed skin models are suitable test systems for toxicity testing and for basic investigations on (patho-)physiological aspects of human skin. Reconstructed human skin, however, has clear limitations such as the lack of immune cells and a significantly weaker skin barrier function compared to native human skin. Potential reasons for the latter might be the lack of mechanical forces during skin model cultivation which is performed classically in static well-plate setups. Mechanical forces and shear stress have a major impact on tissue formation and, hence, tissue engineering...
June 27, 2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28651180/does-a-short-term-exposure-to-atrazine-provoke-cellular-senescence-in-chlamydomonas-reinhardtii
#4
Marta Esperanza, Magali Houde, Marta Seoane, Ángeles Cid, Carmen Rioboo
The aim of this study was to investigate the impacts and modes of action of a chemical and nutrient deprivation on the cellular senescence process of Chlamydomonas reinhardtii. Several molecular and cellular parameters related to senescence phenomena were monitored in C. reinhardtii cells exposed for 24h to a sublethal concentration (0.25μM) of the herbicide atrazine and in unexposed 96h cells in an early stationary phase of growth. All endpoints showed the same pattern of response between treatments, except for the intracellular level of calcium, where a significant increase was observed in 24h-exposed cultures compared to 24h-log controls...
June 19, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28651156/alterations-in-aspergillus-brasiliensis-niger-atcc-9642-membranes-associated-to-metabolism-modifications-during-application-of-low-intensity-electric-current
#5
Nancy Velasco-Alvarez, Mariano Gutiérrez-Rojas, Ignacio González
The effects of electric current on membranes associated with metabolism modifications in Aspergillus brasiliensis (niger) ATCC 9642 were studied. A 450-mL electrochemical cell with titanium ruthenium-oxide coated electrodes and packed with 15g of perlite, as inert support, was inoculated with A. brasiliensis spores and incubated in a solid inert-substrate culture (12 d; 30°C). Then, 4.5days after starting the culture, a current of 0.42mAcm(-2) was applied for 24h. The application of low-intensity electric current increased the molecular oxygen consumption rate in the mitochondrial respiratory chain, resulting in high concentrations of reactive oxygen species, promoting high lipoperoxidation levels, according to measured malondialdehyde, and consequent alterations in membrane permeability explained the high n-hexadecane (HXD) degradation rates observed here (4...
June 19, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28651148/aldo-keto-reductase-activity-after-diethylhexyl-phthalate-exposure-in-eutopic-and-ectopic-endometrial-cells
#6
La Yeon Kim, Mee Ran Kim, Jang Heub Kim, Hyun Hee Cho
OBJECTIVE: Endometriosis is a multifactorial gynaecological disease in reproductive-age women. Endometriotic tissue is characterized by high prostaglandin levels and progesterone resistance. Human aldo-keto reductases (AKRs) convert progesterone to a less potent metabolite and cause progesterone resistance. Therefore, in this study, we evaluated whether diethylhexyl phthalate (DEHP) alters AKR expression in human ectopic and eutopic endometrium. STUDY DESIGN: We used microarrays and western blotting to study the effects of DEHP, and checked the presence of AKR in endometriosis patients by enzyme-linked immunosorbent assay (ELISA)...
May 22, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28651114/isolation-and-functional-interrogation-of-adult-human-prostate-epithelial-stem-cells-at-single-cell-resolution
#7
Wen-Yang Hu, Dan-Ping Hu, Lishi Xie, Ye Li, Shyama Majumdar, Larisa Nonn, Hong Hu, Toshi Shioda, Gail S Prins
Using primary cultures of normal human prostate epithelial cells, we developed a novel prostasphere-based, label-retention assay that permits identification and isolation of stem cells at a single cell level. Their bona fide stem cell nature was corroborated using in vitro and in vivo regenerative assays and documentation of symmetric/asymmetric division. Robust WNT10B and KRT13 levels without E-cadherin or KRT14 staining distinguished individual stem cells from daughter progenitors in spheroids. Following FACS to isolate label-retaining stem cells from label-free progenitors, RNA-seq identified unique gene signatures for the separate populations which may serve as useful biomarkers...
June 16, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28651104/autophagy-is-an-important-event-for-low-dose-cytarabine-treatment-in-acute-myeloid-leukemia-cells
#8
Liyun Chen, Pei Guo, Yunxiang Zhang, Xiaoyang Li, Peimin Jia, Jianhua Tong, Junmin Li
Cytarabine (Ara-c) has been an important agent in acute myeloid leukemia (AML) treatment for more than 40 years. While, the mechanisms underlying low dose cytarabine (LD Ara-c) is poorly understood. In this study, we investigated the therapeutic effect of LD Ara-C in vitro. U937 and HEL cell lines were treated with increasing dose of Ara-C and showed growth inhibition rates in a time and dose-dependent manner. Treatment with LD Ara-C (50nM) induced a time-dependent increase in expression of microtubule-associated protein light chain 3 (LC3) and beclin1, but degradation of sequestosome1 (p62) in both U937 and HEL cells...
June 16, 2017: Leukemia Research
https://www.readbyqxmd.com/read/28651094/in-vitro-susceptibility-of-brachyspira-hyodysenteriae-to-a-commercial-citrus-fruit-extract
#9
Pedro J G de Nova, Ana Carvajal, Miguel Prieto, Pedro Rubio
Brachyspira hyodysenteriae is the main etiological agent of swine dysentery (SD). Nowadays, treatment and control of SD is increasingly difficult due to the emergence of antimicrobial resistance together with the restrictions on the use of antibiotics in veterinary practice. The aim of this study was to evaluate, as an alternative in the control of this disease, the antimicrobial activity and the main mechanism of action of BIOCITRO, a citrus extract commercialized as raw material and used as feed additive, against B...
June 20, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28650862/the-potential-combinational-effect-of-mir-34a-with-celecoxib-in-osteosarcoma
#10
Xia Chen, Dan Peng, Yi Shen, Bo Liu, Hongkang Zhou, Huai Tao, Jufang Huang
Advanced osteosarcoma (OS) is usually treated by preoperative and postoperative chemotherapy, but there are a very limited number of active agents. Celecoxib (Cel) is a COX-2-selective nonsteroidal anti-inflammatory drug and its antitumoral effect has been shown widely in a variety of cancers including OS cells in vitro. However, the potential combinational effect of Cel with other biological therapy has not been reported in OS cells. In this study, the effects of Cel, miR-34a mimics, and their combination on cell proliferation (MTT assay), migration (in-vitro scratch assay), invasion (transwell assay), mRNA (real-time PCR), and protein (Western blot) expression of associated signal transductions were investigated in cultured MG63 cells...
June 23, 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/28650731/combination-treatments-with-luteolin-and-fisetin-enhance-anti-inflammatory-effects-in-high-glucose-treated-thp-1-cells-through-histone-acetyltransferase-histone-deacetylase-regulation
#11
Arang Kim, Jung-Mi Yun
Hyperglycemia leads to diabetes and its diabetic complications. In this study, we investigated the synergistic effects of luteolin and fisetin on proinflammatory cytokine secretion and its underlying epigenetic regulation in human monocytes exposed to hyperglycemic (HG) concentrations. Human monocytic cells (THP-1) were cultured under controlled (14.5 mM mannitol), normoglycemic (5.5 mM glucose), or HG (20 mM glucose) conditions in the absence or presence of the two phytochemicals for 48 h. Whereas HG conditions significantly induced histone acetylation, nuclear factor-kappa B (NF-κB) activation, interleukin 6, and tumor necrosis factor-α release from THP-1 cells; combination treatments with the two phytochemicals (500 nM fisetin, and l μM and 500 nM luteolin) suppressed NF-κB activity and inflammatory cytokine release...
June 26, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28650630/waterborne-electrospinning-of-poly-n-isopropyl-acrylamide-by-control-of-environmental-parameters
#12
Ella Schoolaert, Paulien Ryckx, Jozefien Geltmeyer, Samarendra Maji, Paul H M Van Steenberge, Dagmar R D'hooge, Richard Hoogenboom, Karen De Clerck
With increasing toxicity and environmental concerns, electrospinning from water, i.e. waterborne electrospinning, is crucial to further exploit the resulting nanofiber potential. Most water-soluble polymers have the inherent limitation of resulting in water-soluble nanofibers and a tedious chemical cross-linking step is required to reach stable nanofibers. An interesting alternative route is the use of thermoresponsive polymers, such as poly(N-isopropyl acrylamide) (PNIPAM), as they are water-soluble beneath their lower critical solution temperature (LCST) allowing low temperature electrospinning while the obtained nanofibers are water-stable above the LCST...
June 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28650568/enhanced-functional-properties-of-human-limbal-stem-cells-by-inhibition-of-the-mir-31-fih-1-p21-axis
#13
Zhiping Liu, Weijiao Zhan, Minzhi Zeng, Jinghong Chen, Huyong Zou, Zhiqun Min
OBJECTIVE: On the basis of the functional roles of the embryonic stem cell niche (ESCN) in the human limbal stem cells (LSCs), we proposed to explore the potential roles of microRNAs in regulating the self-renewal and differentiation of LSCs cultured in the ESCN. METHODS: The LSCs were cultured in different media, either in CnT-20 media or in CnT-20 + 20% ES culture supernatant (ESC-CM). The LSCs cultured in ESC-CM were then transfected with microRNA-31 (miR-31) mimic or antago-31...
June 26, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28650469/a-compartmentalized-phosphoinositide-signaling-axis-at-cilia-is-regulated-by-inpp5e-to-maintain-cilia-and-promote-sonic-hedgehog-medulloblastoma
#14
S E Conduit, V Ramaswamy, M Remke, D N Watkins, B J Wainwright, M D Taylor, C A Mitchell, J M Dyson
Sonic Hedgehog (SHH) signaling at primary cilia drives the proliferation and progression of a subset of medulloblastomas, the most common malignant paediatric brain tumor. Severe side effects associated with conventional treatments and resistance to targeted therapies has led to the need for new strategies. SHH signaling is dependent on primary cilia for signal transduction suggesting the potential for cilia destabilizing mechanisms as a therapeutic target. INPP5E is an inositol polyphosphate 5-phosphatase that hydrolyses PtdIns(4,5)P2 and more potently, the phosphoinositide (PI) 3-kinase product PtdIns(3,4,5)P3...
June 26, 2017: Oncogene
https://www.readbyqxmd.com/read/28650461/a-light-and-calcium-gated-transcription-factor-for-imaging-and-manipulating-activated-neurons
#15
Wenjing Wang, Craig P Wildes, Tanyaporn Pattarabanjird, Mateo I Sanchez, Gordon F Glober, Gillian A Matthews, Kay M Tye, Alice Y Ting
Activity remodels neurons, altering their molecular, structural, and electrical characteristics. To enable the selective characterization and manipulation of these neurons, we present FLARE, an engineered transcription factor that drives expression of fluorescent proteins, opsins, and other genetically encoded tools only in the subset of neurons that experienced activity during a user-defined time window. FLARE senses the coincidence of elevated cytosolic calcium and externally applied blue light, which together produce translocation of a membrane-anchored transcription factor to the nucleus to drive expression of any transgene...
June 26, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28650446/exosomes-from-plasmodium-infected-hosts-inhibit-tumor-angiogenesis-in-a-murine-lewis-lung-cancer-model
#16
Y Yang, Q Liu, J Lu, D Adah, S Yu, S Zhao, Y Yao, L Qin, L Qin, X Chen
Previous research to investigate the interaction between malaria infection and tumor progression has revealed that malaria infection can potentiate host immune response against tumor in tumor-bearing mice. Exosomes may play key roles in disseminating pathogenic host-derived molecules during infection because several studies have shown the involvement and roles of extracellular vesicles in cell-cell communication. However, the role of exosomes generated during Plasmodium infection in tumor growth, progression and angiogenesis has not been studied either in animals or in the clinics...
June 26, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28650390/derivation-and-characterization-of-a-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line
#17
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
OBJECTIVES: The aim of this study was to identify an epithelial cell line isolated from the spontaneous differentiation of totipotent pig epiblast cells. METHODS: PICM-31 and its colony-cloned derivative cell line, PICM-31A, were established from the culture and differentiation of an epiblast mass isolated from an 8-day-old pig blastocyst. The cell lines were analyzed by transmission electron microscopy, marker gene expression, and mass spectroscopy-based proteomics...
June 22, 2017: Pancreas
https://www.readbyqxmd.com/read/28650106/dok3-modulates-bone-remodeling-by-negatively-regulating-osteoclastogenesis-and-positively-regulating-osteoblastogenesis
#18
Xiaofeng Cai, Junjie Xing, Courtney L Long, Qisheng Peng, Mary Beth Humphrey
Osteoclastogenesis is essential for bone remodeling and normal skeletal maintenance. RANKL promotes osteoclast differentiation and function but requires costimulation of immunoreceptor tyrosine-based activation motif (ITAM)-coupled immunoreceptors. Triggering receptor expressed on myeloid cells-2 (TREM2) coupled to ITAM-adaptor protein DNAX activation protein 12kDA (DAP12) provides costimulation of intracellular calcium signaling during osteoclastogenesis. Previously, we found that downstream of kinase-3 (DOK3) physically associates with DAP12 to inhibit toll-like receptor (TLR)-induced inflammatory signaling in macrophages...
June 26, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28650004/a-collagen-telopeptide-binding-peptide-shows-potential-in-aiding-collagen-bundle-formation-and-fibril-orientation
#19
Wenyu Yang, Lin Li, Guanghao Su, Zhe Zhang, Yiting Cao, Xuemin Li, Yanping Shi, Qiqing Zhang
Type I collagen, the main component of the extracellular matrix (ECM), assembles into bundles, which are then arranged in an orderly manner in many tissues. Various in vitro methods have been developed to mimic this native characteristic to create aligned, collagen-based materials for constructing tissue-engineered organs. In this study, we examined a new strategy to enhance collagen bundle formation and fibril orientation through administration of a collagen telopeptide binding peptide (CTBP). We found that CTBP, or a double-armed CTBP-PEG-CTBP derivative, could interact with both individual collagen molecules and fibrils...
June 26, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28649933/bone-mesenchymal-stem-cell-conditioned-medium-decreases-the-generation-of-astrocytes-during-the-process-of-neural-stem-cells-differentiation
#20
Huang Fang, Peiwen Song, Yuening Shen, Cailiang Shen, Xiaoying Liu
OBJECTIVES: The aim of this study was to investigate the effect of bone mesenchymal stem cell (BMSC) conditioned medium (CM) and Bone morphogenetic protein-4 (BMP-4) on the generation of astrocytes during the process of NSCs differentiation. DESIGN: Neural stem cells (NSCs) were grown under different culture conditions. SETTING: The First Affiliated Hospital of Anhui Medical University, Hefei, China. OUTCOME MEASURES: The study consisted of four groups: NSCs cultured under control conditions (group 1) or with the addition of BMSC-CM (group 2);(BMP-4) (group 3) or both (group 4)...
June 26, 2017: Journal of Spinal Cord Medicine
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