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https://www.readbyqxmd.com/read/28821136/discovery-of-enzymatically-depolymerized-heparins-capable-of-treating-bleomycin-induced-pulmonary-injury-and-fibrosis-in-mice
#1
Yishu Yan, Shanshan Du, Yang Ji, Nan Su, Yi Wang, Xiang Mei, Wenming Zhu, Dong He, Yuan Lu, Chong Zhang, Xin-Hui Xing
Heparin has recently been shown to slow down idiopathic pulmonary fibrosis (IPF) process and improve survival of patients in some cases. To improve the anti-IPF function while minimizing their side effects, we developed heparin libraries with different structures depolymerized by single or combined heparinases, and systematically screened the efficacy of the different heparins for treatment of Bleomycin-induced pulmonary injury and fibrosis using mice model. Then we characterized the structural properties of the components capable of treating pulmonary injury and fibrosis by use of chip-based amide hydrophilic interaction chromatography (HILIC)-fourier transform (FT)-ESI-MS, polyacrylamide gel electrophoresis (PAGE), and high performance liquid chromatography (HPLC)...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821082/recycling-of-pre-hydrolysis-liquor-to-improve-the-concentrations-of-hemicellulosic-saccharides-during-water-pre-hydrolysis-of-aspen-woodchips
#2
Zongquan Li, Jungang Jiang, Yingjuan Fu, Zhaojiang Wang, Menghua Qin
In this study, the pre-hydrolysis liquor (PHL) was recycled during aspen chip water pre-hydrolysis, and the effects of PHL recycling on the extraction and accumulation of the hemicellulosic saccharides especially that with high molecular weight in the PHL were studied. The results showed that the concentration of hemicellulose saccharides in PHL depended on the pre-hydrolysis temperature and PHL recycling times. Compared to the unrecycled PHL, the concentration of hemicellulosic saccharides in PHL increased significantly when recycling PHL once or twice at 170°C...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820988/integrating-printed-microfluidics-with-silicon-photomultipliers-for-miniaturised-and-highly-sensitive-atp-bioluminescence-detection
#3
M F Santangelo, S Libertino, A P F Turner, D Filippini, W C Mak
Bioluminescence has been widely used for important biosensing applications such as the measurement of adenosine triphosphate (ATP), the energy unit in biological systems and an indicator of vital processes. The current technology for detection is mainly based on large equipment such as readers and imaging systems, which require intensive and time-consuming procedures. A miniaturised bioluminescence sensing system, which would allow sensitive and continuous monitoring of ATP, with an integrated and low-cost disposable microfluidic chamber for handling of biological samples, is highly desirable...
July 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28820490/the-potentiodynamic-bottom-up-growth-of-the-tin-oxide-nanostructured-layer-for-gas-analytical-multisensor-array-chips
#4
Fedor S Fedorov, Dmitry Podgainov, Alexey Varezhnikov, Andrey Lashkov, Michail Gorshenkov, Igor Burmistrov, Martin Sommer, Victor Sysoev
We report a deposition of the tin oxide/hydroxide nanostructured layer by the potentiodynamic method from acidic nitrate solutions directly over the substrate, equipped with multiple strip electrodes which is employed as a gas-analytical multisensor array chip. The electrochemical synthesis is set to favor the growth of the tin oxide/hydroxide phase, while the appearance of metallic Sn is suppressed by cycling. The as-synthesized tin oxide/hydroxide layer is characterized by mesoporous morphology with grains, 250-300 nm diameter, which are further crystallized into fine SnO₂ poly-nanocrystals following heating to 300 °C for 24 h just on the chip...
August 18, 2017: Sensors
https://www.readbyqxmd.com/read/28820213/chip-scale-alignment-of-long-dna-nanofibers-on-a-patterned-self-assembled-monolayer
#5
J Xia, M Su
Controlled alignment of long DNA nanofibers is challenging. This communication reports a method to align human genomic DNA with nearly unlimited length using lithographically produced micro-patterns of self-assembled monolayers (SAMs) with positively charged terminal groups. The micro-patterns act as local DNA reservoirs to supply DNAs for nanofiber formation, and can also stretch and align DNA nanofibers to form an ordered array by controlling the dewetting profile. By reducing the size and inter-patch distance of a micro-patch, a nearly uniform array of long DNA nanofibers can be patterned over a large area...
August 18, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28819974/room-temperature-synthesis-of-mn-doped-cesium-lead-halide-quantum-dots-with-high-mn-substitution-ratio
#6
Jingrun Zhu, Xiaoling Yang, Yihua Zhu, Yuanwei Wang, Jin Cai, Jianhua Shen, Luyi Sun, Chunzhong Li
Here we report the room temperature, atmospheric synthesis of Mn-doped cesium lead halide (CsPbX3) perovskite QDs. The synthesis is performed without any sort of protection and the dual-color emission mechanism is revealed by density functional theory. The Mn concentration reaches a maximum atomic percentage of 37.73 at%, which is significantly higher in comparison to those achieved in earlier reports via high temperature hot injection method. The optical properties of as-prepared nanocrystals (NCs) remain consistent even after several months...
August 18, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28819973/mobile-platform-for-multiplexed-detection-and-differentiation-of-disease-specific-nucleic-acid-sequences-using-microfluidic-loop-mediated-isothermal-amplification-and-smartphone-detection
#7
Weili Chen, Hojeong Yu, Fu Sun, Akid Ornob, Ryan Brisbin, Anurup Ganguli, Vinay Kumar Vemuri, Piotr Strzebonski, Guangzhe Cui, Karen J Allen, Smit A Desai, Weiran Lin, David M Nash, David Hirschberg, Ian Brooks, Rashid Bashir, Brian T Cunningham
New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld "cradle" that interfaces the phone with a silicon-based microfluidic chip embedded within a credit card-sized cartridge...
August 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28819843/microfilter-based-capture-and-release-of-viable-circulating-tumor-cells
#8
Siddarth Rawal, Zheng Ao, Ram H Datar, Ashutosh Agarwal
Microfilters with slot-pore geometry can be used for size-based capture of circulating tumor cells (CTC) from the blood of cancer patients. The slot pore geometry reduces the shear stress that the cells would typically experience during filtration process and allows the cells to remain viable. The microfilter provides a platform capable of high CTC capture efficiency; however, the release of these cells from the filter following capture is nontrivial, possibly due to the strong nonspecific electrostatic adhesion of CTC to the microfilter surface...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28819841/microscale-laminar-vortices-for-high-purity-extraction-and-release-of-circulating-tumor-cells
#9
Soojung Claire Hur, James Che, Dino Di Carlo
Circulating tumor cells (CTCs) are disseminated tumor cells that reflect the tumors of origin and can provide a liquid biopsy that would potentially enable noninvasive tumor profiling, treatment monitoring, and identification of targeted treatments. Accurate and rapid purification of CTCs holds great potential to improve cancer care but the task remains technically challenging. Microfluidic isolation of CTCs within microscale vortices enables high-throughput and size-based purification of rare CTCs from bodily fluids...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28819840/microfluidic-based-enrichment-and-retrieval-of-circulating-tumor-cells-for-rt-pcr-analysis
#10
Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, Yixin Wang
Molecular analysis of circulating tumor cells (CTCs) is hindered by low sensitivity and high level of background leukocytes of currently available CTC enrichment technologies. We have developed a novel device to enrich and retrieve CTCs from blood samples by using a microfluidic chip. The Celsee PREP100 device captures CTCs with high sensitivity and allows the captured CTCs to be retrieved for molecular analysis. It uses the microfluidic chip which has approximately 56,320 capture chambers. Based on differences in cell size and deformability, each chamber ensures that small blood escape while larger CTCs of varying sizes are trapped and isolated in the chambers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28819837/microfluidic-capture-and-multiplex-immunofluorescence-of-circulating-tumor-cells-to-identify-cancer-of-origin
#11
Chian-Hui Lai, Ying-Chih Chang
Circulating tumor cells (CTCs) are an important biomarker and their analysis can be considered a form of "liquid biopsy." The purpose of this book chapter is to describe the use of the 4-channel CMx (cells captured in maximum) microfluidic chip, containing special micropatterns coated with an antibody-conjugated supported lipid bilayer (SLB) on its surface, to capture and isolate CTCs from the blood of cancer patients. Captured CTCs are subsequently released by an air foam to an immunofluorescence (IF) staining panel that enables further analysis, including the identification of the primary cancer source of the CTCs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28819670/mimicking-wettability-alterations-using-temperature-gradients-for-water-nanodroplets
#12
Chirodeep Bakli, Sree Hari P D, Suman Chakraborty
A sessile droplet or a film usually moves from hotter regions to colder regions, due to variations in interfacial tension. This, known as the so-called Marangoni effect, is true for most pure liquids like water for which the surface tension decreases with an increase in temperature. In stark contrast to this existing understanding, we bring forth the coupled effect of wettability and temperature gradients on the dynamics of the three-phase contact line. By simultaneously tracking the dynamic evolution of the three-phase contact line due to the evaporation and diffusion of molecules through molecular dynamics simulations, we explore the coterminous effects of the change of surface tension coefficients and wetting parameters with temperature on sessile droplets residing on surfaces with different wettabilities...
August 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/28819645/3d-imaging-of-optically-cleared-tissue-using-a-simplified-clarity-method-and-on-chip-microscopy
#13
Yibo Zhang, Yoonjung Shin, Kevin Sung, Sam Yang, Harrison Chen, Hongda Wang, Da Teng, Yair Rivenson, Rajan P Kulkarni, Aydogan Ozcan
High-throughput sectioning and optical imaging of tissue samples using traditional immunohistochemical techniques can be costly and inaccessible in resource-limited areas. We demonstrate three-dimensional (3D) imaging and phenotyping in optically transparent tissue using lens-free holographic on-chip microscopy as a low-cost, simple, and high-throughput alternative to conventional approaches. The tissue sample is passively cleared using a simplified CLARITY method and stained using 3,3'-diaminobenzidine to target cells of interest, enabling bright-field optical imaging and 3D sectioning of thick samples...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28819442/interferon-%C3%AE-promotes-the-expression-of-cancer-stem-cell-markers-in-oral-squamous-cell-carcinoma
#14
Hailong Ma, Shufang Jin, Wenyi Yang, Zhuowei Tian, Shuli Liu, Yang Wang, Ge Zhou, Mei Zhao, Shalva Gvetadze, Zhiyuan Zhang, Jingzhou Hu
Objectives: IFNα can stimulate an antitumor immune response and has a direct inhibition on cancer cells. This study is to test whether IFNα can activate dormant cancer stem cell (CSC) in oral squamous cell carcinoma (OSCC) to facilitate their elimination by chemotherapy. Materials and methods: Nude mouse transplantation tumor model was established and administrated with IFNα and saline. The influence on CD44 and ALDH1A1 expression under IFNα treatment was detected by in vivo experiments. Flow cytometry, western blot, and immunofluorescence were used to detect the expression of CD44 and ALDH1A1 after INFa treatment in OSCC cell lines...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28819390/fluorofenidone-inhibits-the-proliferation-of-lung-adenocarcinoma-cells
#15
Zheng-Hao Deng, Jie Meng, Juan Tang, Gao-Yun Hu, Li-Jian Tao
Background: Lung carcinoma is the leading cause of malignant tumor related mortality in China in recent decades, and the development of new and effective therapies for patients with advanced lung carcinoma is needed. We recently found that fluorofenidone (FD), a newly developed pyridine compound, reduced the activation of Stat3 (Signal transducer and activator of transcription 3) in fibroblasts. Stat3 plays a crucial role in the development of lung cancer and may represent a new therapeutic target. In this study, we examined the effect of FD on human lung adenocarcinoma cells in vivo and in vitro...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28819310/genome-wide-target-enrichment-aided-chip-design-a-66%C3%A2-k-snp-chip-for-cashmere-goat
#16
Xian Qiao, Rui Su, Yang Wang, Ruijun Wang, Ting Yang, Xiaokai Li, Wei Chen, Shiyang He, Yu Jiang, Qiwu Xu, Wenting Wan, Yaolei Zhang, Wenguang Zhang, Jiang Chen, Bin Liu, Xin Liu, Yixing Fan, Duoyuan Chen, Huaizhi Jiang, Dongming Fang, Zhihong Liu, Xiaowen Wang, Yanjun Zhang, Danqing Mao, Zhiying Wang, Ran Di, Qianjun Zhao, Tao Zhong, Huanming Yang, Jian Wang, Wen Wang, Yang Dong, Xiaoli Chen, Xun Xu, Jinquan Li
Compared with the commercially available single nucleotide polymorphism (SNP) chip based on the Bead Chip technology, the solution hybrid selection (SHS)-based target enrichment SNP chip is not only design-flexible, but also cost-effective for genotype sequencing. In this study, we propose to design an animal SNP chip using the SHS-based target enrichment strategy for the first time. As an update to the international collaboration on goat research, a 66 K SNP chip for cashmere goat was created from the whole-genome sequencing data of 73 individuals...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819269/characteristic-assessment-of-diesel-degrading-bacteria-immobilized-on-natural-organic-carriers-in-marine-environment-the-degradation-activity-and-nutrient
#17
Jianliang Xue, Yanan Wu, Zhixiu Liu, Menglu Li, Xiyu Sun, Huajun Wang, Bing Liu
Oil spill has led to severe environmental and ecological problems. Due to the harsh environmental conditions, the bioremediation technology is not successfully used to remedy the oil spill in marine environment. In this study, immobilization technology was used to immobilize bacteria on natural organic carriers (i.e., wood chips and maize straw). The higher surface area of in wood chips leads to larger biomass density (0.0242 gVSS/g) than that of maize straw of 0.0097 gVSS/g carrier. Compared with biodegradation efficiency of free bacteria (44...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819205/fast-wide-volume-functional-imaging-of-engineered-in-vitro-brain-tissues
#18
G Palazzolo, M Moroni, A Soloperto, G Aletti, G Naldi, M Vassalli, T Nieus, F Difato
The need for in vitro models that mimic the human brain to replace animal testing and allow high-throughput screening has driven scientists to develop new tools that reproduce tissue-like features on a chip. Three-dimensional (3D) in vitro cultures are emerging as an unmatched platform that preserves the complexity of cell-to-cell connections within a tissue, improves cell survival, and boosts neuronal differentiation. In this context, new and flexible imaging approaches are required to monitor the functional states of 3D networks...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818871/sexual-dimorphism-of-the-pulmonary-transcriptome-in-neonatal-hyperoxic-lung-injury-identification-of-angiogenesis-as-a-key-pathway
#19
Cristian Coarfa, Yuhao Zhang, Suman Maity, Dimuthu Perera, Weiwu Jiang, Lihua Wang, Xanthi Couroucli, Bhagavatula Moorthy, Krithika Lingappan
Bronchopulmonary dysplasia (BPD) is characterized by impaired alveolar secondary septation and vascular growth. Exposure to high concentrations of oxygen (hyperoxia) contributes to the development of BPD. Male sex is considered an independent risk factor for the development of BPD. The reasons underlying sexually dimorphic outcomes in premature neonates are not known. We hypothesized that sex-specific modulation of biological processes in the lung under hyperoxic conditions, contributes to sex-based differences...
August 17, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28818515/integrative-analysis-of-hepatic-microrna-and-mrna-to-identify-potential-biological-pathways-associated-with-monocrotaline-induced-liver-injury-in-mice
#20
Zhenlin Huang, Minwei Chen, Jiaqi Zhang, Yuchen Sheng, Lili Ji
Pyrrolizidine alkaloids (PAs) are a type of natural hepatotoxic compounds. Monocrotaline (MCT), belongs to PAs, is a main compound distributed in medicinal herb Crotalaria ferruginea Grah. ex Benth. This study aims to identify the potential biological signaling pathway associated with MCT-induced liver injury by analyzing the integrative altered hepatic microRNA (miRNA) and mRNA expression profile. C57BL/6 mice were orally given with MCT (270, 330mg/kg). Serum alanine/aspartate aminotransferase (ALT/AST) activity, total bilirubin (TBil) amount and liver histological evaluation showed the liver injury induced by MCT...
August 14, 2017: Toxicology and Applied Pharmacology
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