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https://www.readbyqxmd.com/read/29778422/sp1-induced-upregulation-of-lncrna-pandar-predicts-adverse-phenotypes-in-retinoblastoma-and-regulates-cell-growth-and-apoptosis-in-vitro-and-in-vivo
#1
Lijie Sheng, Jing Wu, Xuewu Gong, Duo Dong, Xiaoxue Sun
Retinoblastoma (RB) is an intraocular malignancy for children and has a high mortality rate. Long non-coding RNAs (lncRNAs) are emerging as gene regulators and biomarkers in various malignancies. PANDAR is a novel cancer-related lncRNA that dysregulated in several types of cancers. However, its clinical value and potential effects on RB remains unclear. RT-qPCR was used to assess the relative expression of PANDAR in RB tissues and cells. Additionally, chromatin immunoprecipitation (ChIP) and luciferase reporter assays were performed to investigate whether SP1 could bind to the promoter region of PANDAR and activate its transcription...
May 17, 2018: Gene
https://www.readbyqxmd.com/read/29777736/edge-strength-of-cad-cam-materials
#2
Maria Pfeilschifter, Verena Preis, Michael Behr, Martin Rosentritt
OBJECTIVES: To investigate the edge force of CAD/CAM materials as a function of (a) material, (b) thickness, and (c) distance from the margin. METHODS: Materials intended for processing with CAD/CAM were investigated: eight resin composites, one resin-infiltrated ceramic, and a clinically proven lithiumdisilicate ceramic (reference). To measure edge force (that is, load to failure/crack), plates (d = 1 mm) were fixed and loaded with a Vickers diamond indenter (1 mm/min, Zwick 1446) at a distance of 0...
May 16, 2018: Journal of Dentistry
https://www.readbyqxmd.com/read/29777597/microdevice-based-solid-phase-polymerase-chain-reaction-for-rapid-detection-of-pathogenic-microorganisms
#3
Quang Nghia Pham, Kieu The Loan Trinh, Seung Won Jung, Nae Yoon Lee
We demonstrate the integration of DNA amplification and detection functionalities developed on a lab-on-a-chip microdevice utilizing solid-phase polymerase chain reaction (SP-PCR) for point-of-need (PON) DNA analyses. First, the polycarbonate microdevice was fabricated by thermal bonding to contain microchambers as reservoirs for performing SP-PCR. Next, the microchambers were subsequently modified with polyethyleneimine and glutaraldehyde for immobilizing amine-modified forward primers. During SP-PCR, the immobilized forward primers and freely diffusing fluorescence-labeled reverse primers cooperated to generate target amplicons, which remained covalently attached to the microchambers for the fluorescence detection...
May 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29777275/overexpression-of-abcb4-contributes-to-acquired-doxorubicin-resistance-in-breast-cancer-cells-in-vitro
#4
Jia-Feng Huang, Chun-Jie Wen, Guo-Zhi Zhao, Yi Dai, Ying Li, Lan-Xiang Wu, Hong-Hao Zhou
PURPOSE: Doxorubicin is one of the most active agents in the first-line therapy for metastatic breast cancer, but its utility is partially limited by the frequent emergence of doxorubicin resistance. In this study, we aimed to investigate the role of ATP-binding cassette sub-family B, member 4 (ABCB4) in acquired doxorubicin resistance in breast cancer cells, as well as its potential mechanism. METHODS: In doxorubicin-sensitive and -resistant breast cancer cell lines MCF-7 and MDA-MB-231, the expression levels of ABCB4 were detected using real-time quantitative PCR and Western blot analysis, the DNA methylation and histone acetylation status of ABCB4 gene were investigated by bisulfite-sequencing PCR (BSP) and chromatin immunoprecipitation (ChIP) assays, and the doxorubicin sensitivity and intracellular doxorubicin accumulation were observed using cell cytotoxicity assay and flow cytometry...
May 18, 2018: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/29777196/single-measurement-detection-of-individual-cell-ionic-oscillations-using-an-n-type-semiconductor-electrolyte-interface
#5
Mariusz Pietruszka, Monika Olszewska, Lukasz Machura, Edward Rówiński
Pollen tubes are used as models in studies on the type of tip-growth in plants. They are an example of polarised and rapid growth because pollen tubes are able to quickly invade the flower pistil in order to accomplish fertilisation. How different ionic fluxes are perceived, processed or generated in the pollen tube is still not satisfactorily understood. In order to measure the H+ , K+ , Ca2+ and Cl- fluxes of a single pollen tube, we developed an Electrical Lab on a Photovoltaic-Chip (ELoPvC) on which the evolving cell was immersed in an electrolyte of a germination medium...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29777104/optical-wireless-link-between-a-nanoscale-antenna-and-a-transducing-rectenna
#6
Arindam Dasgupta, Marie-Maxime Mennemanteuil, Mickaël Buret, Nicolas Cazier, Gérard Colas-des-Francs, Alexandre Bouhelier
Initiated as a cable-replacement solution, short-range wireless power transfer has rapidly become ubiquitous in the development of modern high-data throughput networking in centimeter to meter accessibility range. Wireless technology is now penetrating a higher level of system integration for chip-to-chip and on-chip radiofrequency interconnects. However, standard CMOS integrated millimeter-wave antennas have typical size commensurable with the operating wavelength, and are thus an unrealistic solution for downsizing transmitters and receivers to the micrometer and nanometer scale...
May 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29776965/developmentally-inspired-human-organs-on-chips
#7
Donald E Ingber
Although initially developed to replace animal testing in drug development, human 'organ on a chip' (organ chip) microfluidic culture technology offers a new tool for studying tissue development and pathophysiology, which has brought us one step closer to carrying out human experimentation in vitro In this Spotlight article, I discuss the central role that developmental biology played in the early stages of organ-chip technology, and how these models have led to new insights into human physiology and disease mechanisms...
May 18, 2018: Development
https://www.readbyqxmd.com/read/29776585/tears-of-wine-the-dance-of-the-droplets
#8
REVIEW
Alex Nikolov, Darsh Wasan, Jongju Lee
For a long time, the phenomenon known as the "tears of wine" was believed to be due only to the surface tension gradient (e.g., the Thomson-Marangoni stress on the fluid/fluid surface's dynamics of wetting) and gravity. We experimentally demonstrated that the wine tear formation is not solely due to the surface tension gradient; instead, the ridge instability triggers the wine tears. Pouring wine into a glass causes a wine film to form on the glass. The film drains down under gravity and a ridge forms at its upper part...
May 5, 2018: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/29776538/rapid-detection-of-calr-type-1-and-type-2-mutations-using-pna-lna-clamping-loop-mediated-isothermal-amplification-on-a-cd-like-microfluidic-chip
#9
Guojun Cao, Jilie Kong, Zhifang Xing, Yigui Tang, Xinju Zhang, Xiao Xu, Zhihua Kang, Xueen Fang, Ming Guan
Bleeding and thrombosis represent common complications in myeloproliferative neoplasms (MPN) and significantly contribute to morbidity and mortality. Molecular markers, including CALR mutations, were considered not only as diagnostic markers, but also as risk factors for bleeding and thrombosis associated with MPN, especially for patients in remote primary hospitals. We sought to develop an easy-to-use assay for the rapid detection of CALR type 1 (CALR-1) and type 2 (CALR-2) mutations in Philadelphia chromosome-negative MPN patients...
September 18, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29776409/calcitriol-downregulates-fibroblast-growth-factor-receptor-1-through-histone-deacetylase-activation-in-hl-1-atrial-myocytes
#10
Ting-Wei Lee, Ting-I Lee, Yung-Kuo Lin, Yu-Hsun Kao, Yi-Jen Chen
BACKGROUND: Fibroblast growth factor (FGF)-2 plays a crucial role in the pathophysiology of cardiovascular diseases (CVDs). FGF-2 was reported to induce cardiac hypertrophy through activation of FGF receptor 1 (FGFR1). Multiple laboratory findings indicate that calcitriol may be a potential treatment for CVDs. In this study, we attempted to investigate whether calcitriol regulates FGFR1 expression to modulate the effects of FGF-2 signaling in cardiac myocytes and explored the potential regulatory mechanism...
May 18, 2018: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29776335/using-genomic-relationship-likelihood-for-parentage-assignment
#11
Kim E Grashei, Jørgen Ødegård, Theo H E Meuwissen
BACKGROUND: Parentage assignment is usually based on a limited number of unlinked, independent genomic markers (microsatellites, low-density single nucleotide polymorphisms (SNPs), etc.). Classical methods for parentage assignment are exclusion-based (i.e. based on loci that violate Mendelian inheritance) or likelihood-based, assuming independent inheritance of loci. For true parent-offspring relations, genotyping errors cause apparent violations of Mendelian inheritance. Thus, the maximum proportion of such violations must be determined, which is complicated by variable call- and genotype error rates among loci and individuals...
May 18, 2018: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/29776334/swi-snf-regulates-half-of-its-targets-without-the-need-of-atp-driven-nucleosome-remodeling-by-brahma
#12
Antonio Jordán-Pla, Simei Yu, Johan Waldholm, Thomas Källman, Ann-Kristin Östlund Farrants, Neus Visa
BACKGROUND: Brahma (BRM) is the only catalytic subunit of the SWI/SNF chromatin-remodeling complex of Drosophila melanogaster. The function of SWI/SNF in transcription has long been attributed to its ability to remodel nucleosomes, which requires the ATPase activity of BRM. However, recent studies have provided evidence for a non-catalytic function of BRM in the transcriptional regulation of a few specific genes. RESULTS: Here we have used RNA-seq and ChIP-seq to identify the BRM target genes in S2 cells, and we have used a catalytically inactive BRM mutant (K804R) that is unable to hydrolyze ATP to investigate the magnitude of the non-catalytic function of BRM in transcription regulation...
May 18, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29775464/biochemical-characterization-of-recbcd-enzyme-from-an-antarctic-pseudomonas-species-and-identification-of-its-cognate-chi-%C3%AF-sequence
#13
Theetha L Pavankumar, Anurag K Sinha, Malay K Ray
Pseudomonas syringae Lz4W RecBCD enzyme, RecBCDPs, is a trimeric protein complex comprised of RecC, RecB, and RecD subunits. RecBCD enzyme is essential for P. syringae growth at low temperature, and it protects cells from low temperature induced replication arrest. In this study, we show that the RecBCDPs enzyme displays distinct biochemical behaviors. Unlike E. coli RecBCD enzyme, the RecD subunit is indispensable for RecBCDPs function. The RecD motor activity is essential for the Chi-like fragments production in P...
2018: PloS One
https://www.readbyqxmd.com/read/29775042/bioinspired-synergy-sensor-chip-of-photonic-crystals-graphene-oxide-for-multi-amines-recognition
#14
Wanjie Ren, Meng Qin, Xiaotian Hu, Fengyu Li, Yuanfeng Wang, Yu Huang, Meng Su, Wenbo Li, Xin Qian, Kang-Lai Tang, Yanlin Song
Benefiting from the integrated functions of cilia and glomeruli in the olfactory system, animals can discriminate various odours even in hostile environments. Inspired by this synergetic system of response and signal processing units, a sensor chip of gra-phene oxide (GO) and photonic crystals (PCs) is fabricated. The GO aerogel functions like the olfactory cilia, which effectively captures the analytes and generates abundant sensing signals for recognition; and the PCs act as the olfactory glomeruli, whose periodic structure enables selective enhancement of the fluorescent signals to realize further signal processing...
May 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29774548/release-of-tgf-%C3%AE-1-into-root-canals-with-various-final-irrigants-in-regenerative-endodontics-an-in-vitro-analysis
#15
Y Chae, M Yang, J R Kim
AIM: To investigate the release of growth factors into the root canal space after various final irrigants during regenerative endodontic procedures. The residual cytotoxic effect of final irrigants on stem cells from the apical papilla (SCAP) was also examined. METHODOLOGY: To measure the release of TGF-β1, root segments (8mm long) were irrigated with 1.5% NaOCl followed by 20 mL of final irrigants; Saline, 17% EDTA, 10% citric acid, 10% or 37% phosphoric acid...
May 17, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29774333/optical-tweezing-using-tunable-optical-lattices-along-a-few-mode-silicon-waveguide
#16
C Pin, J-B Jager, M Tardif, E Picard, E Hadji, F de Fornel, B Cluzel
Fourteen years ago, optical lattices and holographic tweezers were considered as a revolution, allowing for trapping and manipulating multiple particles at the same time using laser light. Since then, near-field optical forces have aroused tremendous interest as they enable efficient trapping of a wide range of objects, from living cells to atoms, in integrated devices. Yet, handling at will multiple objects using a guided light beam remains a challenging task for current on-chip optical trapping techniques...
May 18, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29774084/a-novel-dual-well-array-chip-for-efficiently-trapping-single-cell-in-large-isolated-micro-well-without-complicated-accessory-equipment
#17
Chenyu Wang, Wenwen Liu, Qingquan Wei, Lufeng Ren, Manqing Tan, Yude Yu
Conventional cell-sized well arrays have advantages of high occupancy, simple operation, and low cost for capturing single-cells. However, they have insufficient space for including reagents required for cell treatment or analysis, which restricts the wide application of cell-sized well arrays as a single-cell research tool alone. Here, we present a novel dual-well array chip, which integrates capture-wells (20  μ m in diameter) with reaction-wells (100  μ m in diameter) and describe a flow method for convenient single-cell analysis requiring neither complicated infra-structure nor high expenditure, while enabling highly efficient single cell trapping (75...
May 2018: Biomicrofluidics
https://www.readbyqxmd.com/read/29774079/long-non-coding-rnas-ac026904-1-and-uca1-a-one-two-punch-for-tgf-%C3%AE-induced-snai2-activation-and-epithelial-mesenchymal-transition-in-breast-cancer
#18
Guo-Yin Li, Wei Wang, Jian-Yong Sun, Bo Xin, Xiang Zhang, Ting Wang, Qian-Feng Zhang, Li-Bo Yao, Hua Han, Dai-Ming Fan, An-Gang Yang, Lin-Tao Jia, Lei Wang
Transforming growth factor-β (TGF-β) has received much attention as a major inducer of epithelial-mesenchymal transition (EMT) during cancer progression, mainly by activating a set of pleiotropic transcription factors including SNAI2/Slug. However, the involvement of long non-coding RNAs (lncRNAs) in TGF-β-induced Slug activation and EMT remains largely unknown. Methods: In this study, we used microarray analysis to compare lncRNA expression profiles between TGF-β treated and untreated breast cancer cells...
2018: Theranostics
https://www.readbyqxmd.com/read/29774039/a-membrane-bound-nac-like-transcription-factor-osntl5-represses-the-flowering-in-oryza-sativa
#19
Siyi Guo, Shaojun Dai, Prashant K Singh, Hongyan Wang, Yanan Wang, Jeanie L H Tan, Wanyi Wee, Toshiro Ito
In spite of short-day (SD) nature, rice ( Oryza sativa ) shares a conserved photoperiodic network for flowering control with long-day plants like Arabidopsis thaliana. Flowering or heading is an important agronomic trait in rice. NAC transcription factors (TFs) are well-conserved and one of the largest families of plant TFs. However, their function in flowering or heading time is not well-known yet. A preferential expression of a membrane-bound NAC-like TF OsNTL5 in developing leaves and panicles of rice indicated to us its putative role in flowering...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29773903/circadian-regulator-nr1d2-regulates-glioblastoma-cell-proliferation-and-motility
#20
Min Yu, Wenjing Li, Qianqian Wang, Yan Wang, Fei Lu
Nuclear receptor NR1D2 is originally characterized as the repressor of genes involved in circadian rhythm. Recently, it is documented that NR1D2 is overexpressed in various cancers. However, the pathways and biological functions that NR1D2 involved in cancers remain poorly understood. Here, we reported that NR1D2 was abundant in human glioblastoma (GBM) tissue and cell lines but not primary human astrocytes. Silencing of NR1D2 changed the morphology of GBM cells, inhibited cell proliferation and motility, whereas had no effects on apoptosis...
May 18, 2018: Oncogene
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