keyword
MENU ▼
Read by QxMD icon Read
search

bag3

keyword
https://www.readbyqxmd.com/read/27922674/2-hydroxycinnamaldehyde-induces-apoptosis-through-hsf1-mediated-bag3-expression
#1
Hai-Anh Nguyen, Soo-A Kim
BAG3, a member of BAG co-chaperone family, is induced by stressful stimuli such as heat shock and heavy metals. Through interaction with various binding partners, BAG3 is thought to play a role in cellular adaptive responses against stressful conditions in normal and neoplastic cells. 2'-Hydroxycinnamaldehyde (HCA) is a natural derivative of cinnamaldehyde and has antitumor activity in various cancer cells. In the present study, for the first time, we identified that HCA induced BAG3 expression and BAG3-mediated apoptosis in cancer cells...
December 6, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27884606/bag3-is-a-modular-scaffolding-protein-that-physically-links-heat-shock-protein-70-hsp70-to-the-small-heat-shock-proteins
#2
Jennifer N Rauch, Eric Tse, Rebecca Freilich, Sue-Ann Mok, Leah N Makley, Daniel R Southworth, Jason E Gestwicki
Small heat shock proteins (sHsps) are a family of ATP-independent molecular chaperones that are important for binding and stabilizing unfolded proteins. In this task, the sHsps have been proposed to coordinate with ATP-dependent chaperones, including heat shock protein 70 (Hsp70). However, it is not yet clear how these two important components of the chaperone network are linked. We report that the Hsp70 co-chaperone, BAG3, is a modular, scaffolding factor to bring together sHsps and Hsp70s. Using domain deletions and point mutations, we found that BAG3 uses both of its IPV motifs to interact with sHsps, including Hsp27 (HspB1), αB-crystallin (HspB5), Hsp22 (HspB8), and Hsp20 (HspB6)...
November 21, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27882354/bcl-2-associated-athanogene-3-protects-the-heart-from-ischemia-reperfusion-injury
#3
Feifei Su, Valerie D Myers, Tijana Knezevic, JuFang Wang, Erhe Gao, Muniswamy Madesh, Farzaneh G Tahrir, Manish K Gupta, Jennifer Gordon, Joseph Rabinowitz, Frederick V Ramsey, Douglas G Tilley, Kamel Khalili, Joseph Y Cheung, Arthur M Feldman
Bcl-2-associated athanogene 3 (BAG3) is an evolutionarily conserved protein expressed at high levels in the heart and the vasculature and in many cancers. While altered BAG3 expression has been associated with cardiac dysfunction, its role in ischemia/reperfusion (I/R) is unknown. To test the hypothesis that BAG3 protects the heart from reperfusion injury, in vivo cardiac function was measured in hearts infected with either recombinant adeno-associated virus serotype 9-expressing (rAAV9-expressing) BAG3 or GFP and subjected to I/R...
November 17, 2016: JCI Insight
https://www.readbyqxmd.com/read/27860220/k-bilds-a-kinase-substrate-discovery-tool
#4
D Maheeka Embogama, Mary Kay H Pflum
Kinases catalyze protein phosphorylation to regulate cell signaling events.However, identifying kinase substrates is challenging due to the often low abundance and dynamic nature of protein phosphorylation. Development of novel techniques to identify kinase substrates is necessary. Here, we report K-BILDS (Kinase-catalyzed Biotinylation with Inactivated Lysates for Discovery of Substrates) as a tool to identify direct substrates of a kinase. As a proof of concept, K-BILDS was applied to PKA (cAMP-dependent protein kinase A) using HeLa cell lysates...
November 17, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27777286/interference-with-the-hsf1-hsp70-bag3-pathway-primes-glioma-cells-to-matrix-detachment-and-bh3-mimetic-induced-apoptosis
#5
Patrick Antonietti, Benedikt Linder, Stephanie Hehlgans, Iris C Mildenberger, Michael C Burger, Simone Fulda, Joachim P Steinbach, Florian Gessler, Franz Rodel, Michel Mittelbronn, Donat Kogel
Malignant gliomas exhibit a high intrinsic resistance against stimuli triggering apoptotic cell death. HSF1 (heat shock factor 1) acts as transcription factor upstream of HSP70 and the HSP70 co-chaperone BAG3 that is overexpressed in glioblastoma. To specifically target this resistance mechanism, we applied the selective HSF1 inhibitor KRIBB11 and the HSP70/BAG3 interaction inhibitor YM-1 in combination with the pan-Bcl-2 inhibitor AT-101. Here we demonstrate that lentiviral BAG3 silencing significantly enhances AT-101-induced cell death and reactivates effector caspase-mediated apoptosis in U251 glioma cells with high BAG3 expression, while these sensitizing effects were less pronounced in U343 cells expressing lower BAG3 levels...
October 24, 2016: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/27763645/the-prosurvival-protein-bag3-a-new-participant-in-vascular-homeostasis
#6
Albino Carrizzo, Antonio Damato, Mariateresa Ambrosio, Antonia Falco, Alessandra Rosati, Mario Capunzo, Michele Madonna, Maria C Turco, James L Januzzi, Vincenzo De Laurenzi, Carmine Vecchione
Bcl2-associated athanogene 3 (BAG3), is constitutively expressed in a few normal cell types, including myocytes, peripheral nerves and in the brain, and is also expressed in certain tumors. To date, the main studies about the role of BAG3 are focused on its pro-survival effect in tumors through various mechanisms that vary according to cellular type. Recently, elevated concentrations of a soluble form of BAG3 were described in patients affected by advanced stage of heart failure (HF), identifying BAG3 as a potentially useful biomarker in monitoring HF progression...
October 20, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27756573/the-cochaperone-bag3-coordinates-protein-synthesis-and-autophagy-under-mechanical-strain-through-spatial-regulation-of-mtorc1
#7
Barbara Kathage, Sebastian Gehlert, Anna Ulbricht, Laura Lüdecke, Victor E Tapia, Zacharias Orfanos, Daniela Wenzel, Wilhelm Bloch, Rudolf Volkmer, Bernd K Fleischmann, Dieter O Fürst, Jörg Höhfeld
The cochaperone BAG3 is a central protein homeostasis factor in mechanically strained mammalian cells. It mediates the degradation of unfolded and damaged forms of the actin-crosslinker filamin through chaperone-assisted selective autophagy (CASA). In addition, BAG3 stimulates filamin transcription in order to compensate autophagic disposal and to maintain the actin cytoskeleton under strain. Here we demonstrate that BAG3 coordinates protein synthesis and autophagy through spatial regulation of the mammalian target of rapamycin complex 1 (mTORC1)...
October 15, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27736938/heat-stress-and-lipopolysaccharide-stimulation-of-chicken-macrophage-like-cell-line-activates-expression-of-distinct-sets-of-genes
#8
Anna Slawinska, John C Hsieh, Carl J Schmidt, Susan J Lamont
Acute heat stress requires immediate adjustment of the stressed individual to sudden changes of ambient temperatures. Chickens are particularly sensitive to heat stress due to development of insufficient physiological mechanisms to mitigate its effects. One of the symptoms of heat stress is endotoxemia that results from release of the lipopolysaccharide (LPS) from the guts. Heat-related cytotoxicity is mitigated by the innate immune system, which is comprised mostly of phagocytic cells such as monocytes and macrophages...
2016: PloS One
https://www.readbyqxmd.com/read/27685647/adenofection-a-method-for-studying-the-role-of-molecular-chaperones-in-cellular-morphodynamics-by-depletion-rescue-experiments
#9
Margit Fuchs, Marie-Chloé Boulanger, Herman Lambert, Jacques Landry, Josée N Lavoie
Cellular processes such as mitosis and cell differentiation are governed by changes in cell shape that largely rely on proper remodeling of the cell cytoskeletal structures. This involves the assembly-disassembly of higher-order macromolecular structures at a given time and location, a process that is particularly sensitive to perturbations caused by overexpression of proteins. Methods that can preserve protein homeostasis and maintain near-to-normal cellular morphology are highly desirable to determine the functional contribution of a protein of interest in a wide range of cellular processes...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27633507/cardiac-arrhythmia-and-late-onset-muscle-weakness-caused-by-a-myofibrillar-myopathy-with-unusual-histopathological-features-due-to-a-novel-missense-mutation-in-flnc
#10
D Avila-Smirnow, L Gueneau, S Batonnet-Pichon, F Delort, H-M Bécane, K Claeys, M Beuvin, B Goudeau, J-P Jais, I Nelson, P Richard, R Ben Yaou, N B Romero, K Wahbi, S Mathis, T Voit, D Furst, P van der Ven, R Gil, P Vicart, M Fardeau, G Bonne, A Behin
Myofibrillar myopathies (MFM) are mostly adult-onset diseases characterized by progressive morphological alterations of the muscle fibers beginning in the Z-disk and the presence of protein aggregates in the sarcoplasm. They are mostly caused by mutations in different genes that encode Z-disk proteins, including DES, CRYAB, LDB3, MYOT, FLNC and BAG3. A large family of French origin, presenting an autosomal dominant pattern, characterized by cardiac arrhythmia associated to late-onset muscle weakness, was evaluated to clarify clinical, morphological and genetic diagnosis...
October 2016: Revue Neurologique
https://www.readbyqxmd.com/read/27618136/mitochondrial-dysfunction-in-myofibrillar-myopathy
#11
Amy E Vincent, John P Grady, Mariana C Rocha, Charlotte L Alston, Karolina A Rygiel, Rita Barresi, Robert W Taylor, Doug M Turnbull
Myofibrillar myopathies (MFM) are characterised by focal myofibrillar destruction and accumulation of myofibrillar elements as protein aggregates. They are caused by mutations in the DES, MYOT, CRYAB, FLNC, BAG3, DNAJB6 and ZASP genes as well as other as yet unidentified genes. Previous studies have reported changes in mitochondrial morphology and cellular positioning, as well as clonally-expanded, large-scale mitochondrial DNA (mtDNA) deletions and focal respiratory chain deficiency in muscle of MFM patients...
October 2016: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/27586274/inhibition-of-autophagy-enhances-dynamin-inhibitor-induced-apoptosis-via-promoting-bak-activation-and-mitochondrial-damage-in-human-jurkat-t-cells
#12
Yoon Hee Kim, Ki Yun Kim, Do Youn Jun, Jong-Sik Kim, Young Ho Kim
Treatment of Jurkat T cells with the dynamin inhibitor, myristyl trimethyl ammonium bromides (MiTMAB) caused cytokinesis impairment and apoptotic DNA fragmentation along with down-regulation of anti-apoptotic BAG3 and Mcl-1 levels, Bak activation, mitochondrial membrane potential (Δψm) loss, activation of caspase-9 and -3, and PARP cleavage, without accompanying necrosis. Bcl-xL overexpression completely abrogated these MiTMAB-induced mitochondrial damage and resultant caspase cascade activation, except for impaired cytokinesis and down-regulated BAG3 and Mcl-1 levels...
September 30, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27570075/a-surveillance-function-of-the-hspb8-bag3-hsp70-chaperone-complex-ensures-stress-granule-integrity-and-dynamism
#13
Massimo Ganassi, Daniel Mateju, Ilaria Bigi, Laura Mediani, Ina Poser, Hyun O Lee, Samuel J Seguin, Federica F Morelli, Jonathan Vinet, Giuseppina Leo, Orietta Pansarasa, Cristina Cereda, Angelo Poletti, Simon Alberti, Serena Carra
Stress granules (SGs) are ribonucleoprotein complexes induced by stress. They sequester mRNAs and disassemble when the stress subsides, allowing translation restoration. In amyotrophic lateral sclerosis (ALS), aberrant SGs cannot disassemble and therefore accumulate and are degraded by autophagy. However, the molecular events causing aberrant SG formation and the molecular players regulating this transition are largely unknown. We report that defective ribosomal products (DRiPs) accumulate in SGs and promote a transition into an aberrant state that renders SGs resistant to RNase...
September 1, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27551493/expression-of-the-anti-apoptotic-bag3-protein-in-leg-venous-ulcerative-tissues
#14
N Campitiello, M Faenza, D Pagliara, C Baldi, P Zeppa, A Rosati, C Rubino
No abstract text is available yet for this article.
2016: Cell Death Discovery
https://www.readbyqxmd.com/read/27474739/non-canonical-interactions-between-heat-shock-cognate-protein-70-hsc70-and-bcl2-associated-anthanogene-bag-co-chaperones-are-important-for-client-release
#15
Jennifer N Rauch, Erik R P Zuiderweg, Jason E Gestwicki
Heat shock cognate protein 70 (Hsc70) regulates protein homeostasis through its reversible interactions with client proteins. Hsc70 has two major domains: a nucleotide-binding domain (NBD), that hydrolyzes ATP, and a substrate-binding domain (SBD), where clients are bound. Members of the BAG family of co-chaperones, including Bag1 and Bag3, are known to accelerate release of both ADP and client from Hsc70. The release of nucleotide is known to be mediated by interactions between the conserved BAG domain and the Hsc70 NBD...
September 16, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27456361/high-expression-of-bag3-predicts-a-poor-prognosis-in-human-medulloblastoma
#16
Dong Yang, Ji Zhou, Hao Wang, Yutao Wang, Ge Yang, Yundong Zhang
Bcl2-associated athanogene 3 (BAG3), a co-chaperone of the heat shock protein (Hsp) 70, regulates various physiological and pathological processes. However, its role in human medulloblastoma has not been clarified. First of all, the expression of BAG3 was examined in formalin-fixed, paraffin-embedded specimens by immunohistochemical staining. And then, the prognostic role of BAG3 was analyzed in 51 medulloblastoma samples. Finally, the roles of BAG3 in the proliferation, migration, and invasion of Daoy medulloblastoma cell were investigated using a specific short hairpin RNA (shRNA)...
July 25, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27443559/exome-sequencing-identifies-variants-in-two-genes-encoding-the-lim-proteins-nrap-and-fhl1-in-an-italian-patient-with-bag3-myofibrillar-myopathy
#17
Francesca D'Avila, Mirella Meregalli, Sara Lupoli, Matteo Barcella, Alessandro Orro, Francesca De Santis, Clementina Sitzia, Andrea Farini, Pasqualina D'Ursi, Silvia Erratico, Riccardo Cristofani, Luciano Milanesi, Daniele Braga, Daniele Cusi, Angelo Poletti, Cristina Barlassina, Yvan Torrente
Myofibrillar myopathies (MFMs) are genetically heterogeneous dystrophies characterized by the disintegration of Z-disks and myofibrils and are associated with mutations in genes encoding Z-disk or Z-disk-related proteins. The c.626 C > T (p.P209L) mutation in the BAG3 gene has been described as causative of a subtype of MFM. We report a sporadic case of a 26-year-old Italian woman, affected by MFM with axonal neuropathy, cardiomyopathy, rigid spine, who carries the c.626 C > T mutation in the BAG3 gene...
June 2016: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/27434096/protein-cross-linking-capillary-electrophoresis-for-protein-protein-interaction-analysis
#18
Claire M Ouimet, Hao Shao, Jennifer N Rauch, Mohamed Dawod, Bryce Nordhues, Chad A Dickey, Jason E Gestwicki, Robert T Kennedy
Capillary electrophoresis (CE) has been identified as a useful platform for detecting, quantifying, and screening for modulators of protein-protein interactions (PPIs). In this method, one protein binding partner is labeled with a fluorophore, the protein binding partners are mixed, and then, the complex is separated from free protein to allow direct determination of bound to free ratios. Although it possesses many advantages for PPI studies, the method is limited by the need to have separation conditions that both prevent protein adsorption to capillary and maintain protein interactions during the separation...
August 16, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27414463/bag3-protein-is-over-expressed-in-endometrioid-endometrial-adenocarcinomas
#19
Veronica Esposito, Carlo Baldi, Pio Zeppa, Michelina Festa, Luana Guerriero, Morena d'Avenia, Massimiliano Chetta, Fulvio Zullo, Vincenzo De Laurenzi, Maria Caterina Turco, Alessandra Rosati, Maurizio Guida
Endometrioid adenocarcinomas represent 80% of endometrial carcinomas(1) . Molecular features, including microsatellite instability, mutations of the PTEN, PIK3CA, K-Ras and β-catenin genes(1) and dysregulations in sncRNAs (small non coding RNAs) are described for this disease. However, mechanisms and molecules that determine cell survival and response/resistance to therapy in different subtypes of this tumour are not fully clarified(1) . This article is protected by copyright. All rights reserved.
July 14, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27391596/familial-dilated-cardiomyopathy-caused-by-a-novel-frameshift-in-the-bag3-gene
#20
Rocio Toro, Alexandra Pérez-Serra, Oscar Campuzano, Javier Moncayo-Arlandi, Catarina Allegue, Anna Iglesias, Alipio Mangas, Ramon Brugada
BACKGROUND: Dilated cardiomyopathy, a major cause of chronic heart failure and cardiac transplantation, is characterized by left ventricular or biventricular heart dilatation. In nearly 50% of cases the pathology is inherited, and more than 60 genes have been reported as disease-causing. However, in 30% of familial cases the mutation remains unidentified even after comprehensive genetic analysis. This study clinically and genetically assessed a large Spanish family affected by dilated cardiomyopathy to search for novel variations...
2016: PloS One
keyword
keyword
52541
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"