keyword
MENU ▼
Read by QxMD icon Read
search

bag1

keyword
https://www.readbyqxmd.com/read/27682262/rare-copy-number-alterations-and-copy-neutral-loss-of-heterozygosity-revealed-in-ameloblastomas-by-high-density-whole-genome-microarray-analysis
#1
Marina Gonçalves Diniz, Alessandra Pires Duarte, Rolando A Villacis, Bruna V A Guimarães, Luiz Cláudio Pires Duarte, Sílvia R Rogatto, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes
BACKGROUND: Ameloblastoma (unicystic, UA, or multicystic, MA) is a rare tumor associated with bone destruction and facial deformity. Its malignant counterpart is the ameloblastic carcinoma (AC). The BRAFV600E mutation is highly prevalent in all these tumors subtypes and cannot account for their different clinical behaviors. METHODS: We assessed copy number alterations (CNAs) and copy-neutral loss of heterozygosity (cnLOH) in UA (n = 2), MA (n = 3), and AC (n = 1) using the CytoScan HD Array (Affymetrix) and the BRAFV600E status...
September 28, 2016: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/27633890/evaluation-of-paclitaxel-effects-in-the-pattern-of-expression-of-survival-and-apoptotic-genes-regulators-in-hela-cells
#2
Uğur Serbes, Özlem Dağdeviren Özsöylemez, Gül Özcan
We aim to determine the regulation of apoptosis by paclitaxel-induced and understand cancer dynamics to treatment targets for HeLa cells by identifying of decrease/increase genes expression on HeLa cells. In this study, the anti-tumor effects of Paclitaxel (PAC) on HeLa cells have been studied in order to determine the cellular and molecular mechanisms of these effects. PAC has been applied to HeLa cells in 6 different doses (3, 7.5, 15, 30, 60, 120 nM) for 48 hours and the IC50 dose MTT method, has been determined with apoptic index (AI) DAPI...
September 14, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27524912/two-gene-signature-improves-the-discriminatory-power-of-iaslc-ats-ers-classification-to-predict-the-survival-of-patients-with-early-stage-lung-adenocarcinoma
#3
Yifeng Sun, Likun Hou, Yu Yang, Huikang Xie, Yang Yang, Zhigang Li, Heng Zhao, Wen Gao, Bo Su
BACKGROUND: In this study, we investigated the contribution of a gene expression-based signature (composed of BAG1, BRCA1, CDC6, CDK2AP1, ERBB3, FUT3, IL11, LCK, RND3, SH3BGR) to survival prediction for early-stage lung adenocarcinoma categorized by the new International Association for the Study of Lung Cancer (IASLC)/the American Thoracic Society (ATS)/the European Respiratory Society (ERS) classification. We also aimed to verify whether gene signature improves the risk discrimination of IASLC/ATS/ERS classification in early-stage lung adenocarcinoma...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27514505/coinhibition-of-overexpressed-genes-in-acute-myeloid-leukemia-subtype-m2-by-gold-nanoparticles-functionalized-with-five-antisense-oligonucleotides-and-one-anti-cd33-cd34-aptamer
#4
M A Zaimy, A Jebali, B Bazrafshan, S Mehrtashfar, S Shabani, A Tavakoli, S H Hekmatimoghaddam, A Sarli, H Azizi, P Izadi, B Kazemi, A Shojaei, A Abdalaian, J Tavakkoly-Bazzaz
The aim of this study was to evaluate an engineered nanostructure to silence five important oncogenes, including BAG1, MDM2, Bcl-2, BIRC5 (survivin) and XIAP, in acute myeloid leukemia subtype 2 (AML-M2). The smart nanostructures were functionalized gold nanoparticles (FGNs) containing five antisense oligonucleotides (AOs) and one anti-CD33(+)/CD34(+) aptamer. First, the best AO for each gene was selected with the OligoWalk online software, and then different arrangements of AOs were evaluated with the RNAstructure software...
September 2016: Cancer Gene Therapy
https://www.readbyqxmd.com/read/27474739/non-canonical-interactions-between-heat-shock-cognate-protein-70-hsc70-and-bcl2-associated-anthanogene-bag-co-chaperones-are-important-for-client-release
#5
Jennifer N Rauch, Erik R P Zuiderweg, Jason E Gestwicki
Heat shock cognate protein 70 (Hsc70) regulates protein homeostasis through its reversible interactions with client proteins. Hsc70 has two major domains: a nucleotide-binding domain (NBD), that hydrolyzes ATP, and a substrate-binding domain (SBD), where clients are bound. Members of the BAG family of co-chaperones, including Bag1 and Bag3, are known to accelerate release of both ADP and client from Hsc70. The release of nucleotide is known to be mediated by interactions between the conserved BAG domain and the Hsc70 NBD...
September 16, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27162137/breaking-bag-the-co-chaperone-bag3-in-health-and-disease
#6
REVIEW
Christian Behl
Human BAG (Bcl-2-associated athanogene) proteins form a family of antiapoptotic proteins that currently consists of six members (BAG1-6) all sharing the BAG protein domain from which the name arises. Via this domain, BAG proteins bind to the heat shock protein 70 (Hsp70), thereby acting as a co-chaperone regulating the activity of Hsp70. In addition to their antiapoptotic activity, all human BAG proteins have distinct functions in health and disease, and BAG3 in particular is the focus of many investigations...
August 2016: Trends in Pharmacological Sciences
https://www.readbyqxmd.com/read/27022301/identification-and-prioritization-of-candidate-genes-for-symptom-variability-in-breast-cancer-survivors-based-on-disease-characteristics-at-the-cellular-level
#7
Theresa A Koleck, Yvette P Conley
Research is beginning to suggest that the presence and/or severity of symptoms reported by breast cancer survivors may be associated with disease-related factors of cancer. In this article, we present a novel approach to the identification and prioritization of biologically plausible candidate genes to investigate relationships between genomic variation and symptom variability in breast cancer survivors. Cognitive dysfunction is utilized as a representative breast cancer survivor symptom to elucidate the conceptualization of and justification for our cellular, disease-based approach to address symptom variability in cancer survivors...
2016: Breast Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/26934577/exome-sequencing-of-oral-squamous-cell-carcinoma-in-users-of-arabian-snuff-reveals-novel-candidates-for-driver-genes
#8
Nezar Noor Al-Hebshi, Shiyong Li, Akram Thabet Nasher, Maged El-Setouhy, Rashad Alsanosi, Jan Blancato, Christopher Loffredo
The study sought to identify genetic aberrations driving oral squamous cell carcinoma (OSCC) development among users of shammah, an Arabian preparation of smokeless tobacco. Twenty archival OSCC samples, 15 of which with a history of shammah exposure, were whole-exome sequenced at an average depth of 127×. Somatic mutations were identified using a novel, matched controls-independent filtration algorithm. CODEX and Exomedepth coupled with a novel, Database of Genomic Variant-based filter were employed to call somatic gene-copy number variations...
July 15, 2016: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/26928609/induction-of-autophagy-interferes-the-tachyzoite-to-bradyzoite-transformation-of-toxoplasma-gondii
#9
Xiangzhi Li, Di Chen, Qianqian Hua, Yujing Wan, Lina Zheng, Yangyang Liu, Jiaxin Lin, Changwang Pan, Xin Hu, Feng Tan
Autophagy process in Toxoplasma gondii plays a vital role in regulating parasite survival or death. Thus, once having an understanding of certain effects of autophagy on the transformation of tachyzoite to bradyzoite this will allow us to elucidate the function of autophagy during parasite development. Herein, we used three TgAtg proteins involved in Atg8 conjugation system, TgAtg3, TgAtg7 and TgAtg8 to evaluate the autophagy level in tachyzoite and bradyzoite of Toxoplasma in vitro based on Pru TgAtg7-HA transgenic strains...
April 2016: Parasitology
https://www.readbyqxmd.com/read/26893680/role-of-bcl2-associated-athanogene-in-resistance-to-platinum-based-chemotherapy-in-non-small-cell-lung-cancer
#10
Yadi Wang, Minwen Ha, Jingsong Liu, Ping Li, Wenlu Zhang, Xuan Zhang
The present study aimed to address the pharmacogenetic role of BAG1 in platinum-based chemotherapy in advanced non-small-cell lung cancer (NSCLC) and in cultured human lung adenocarcinoma A549 cells. A total of 108 NSCLC patients (stages I-IIIA) were treated with a standard chemotherapy regimen of cisplatin plus vinorelbine. Additionally, in vitro cultured A549 cells were treated with cisplatin in the presence or absence of tunicamycin. Cell proliferation was determined by MTT assay and protein levels were assessed via western blot analysis...
February 2016: Oncology Letters
https://www.readbyqxmd.com/read/26493122/association-study-of-androgen-signaling-pathway-genes-in-polycystic-ovary-syndrome
#11
Aline Ketefian, Michelle R Jones, Ronald M Krauss, Yii-Der I Chen, Richard S Legro, Ricardo Azziz, Mark O Goodarzi
OBJECTIVE: To evaluate genes involved in androgen receptor (AR) signaling as candidate genes for polycystic ovary syndrome (PCOS). DESIGN: Two groups of women with PCOS and control women (discovery and replication cohorts), were genotyped for single-nucleotide polymorphisms (SNPs) in eight genes for AR chaperones and co-chaperones: HSPA1A, HSPA8, ST13, STIP1, PTGES3, FKBP4, BAG1, and STUB1. Single-nucleotide polymorphisms were tested for association with PCOS status and with androgenic and metabolic parameters...
February 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/26490709/aberrant-autophagic-response-in-the-muscle-of-a-knock-in-mouse-model-of-spinal-and-bulbar-muscular-atrophy
#12
Paola Rusmini, Maria Josefa Polanco, Riccardo Cristofani, Maria Elena Cicardi, Marco Meroni, Mariarita Galbiati, Margherita Piccolella, Elio Messi, Elisa Giorgetti, Andrew P Lieberman, Carmelo Milioto, Anna Rocchi, Tanya Aggarwal, Maria Pennuto, Valeria Crippa, Angelo Poletti
Spinal and bulbar muscular atrophy (SBMA) is characterized by loss of motoneurons and sensory neurons, accompanied by atrophy of muscle cells. SBMA is due to an androgen receptor containing a polyglutamine tract (ARpolyQ) that misfolds and aggregates, thereby perturbing the protein quality control (PQC) system. Using SBMA AR113Q mice we analyzed proteotoxic stress-induced alterations of HSPB8-mediated PQC machinery promoting clearance of misfolded proteins by autophagy. In muscle of symptomatic AR113Q male mice, we found expression upregulation of Pax-7, myogenin, E2-ubiquitin ligase UBE2Q1 and acetylcholine receptor (AchR), but not of MyoD, and of two E3-ligases (MuRF-1 and Cullin3)...
October 22, 2015: Scientific Reports
https://www.readbyqxmd.com/read/26380983/hypoxic-preconditioning-increases-survival-and-pro-angiogenic-capacity-of-human-cord-blood-mesenchymal-stromal-cells-in-vitro
#13
Andreas Matthäus Bader, Kristin Klose, Karen Bieback, Dirk Korinth, Maria Schneider, Martina Seifert, Yeong-Hoon Choi, Andreas Kurtz, Volkmar Falk, Christof Stamm
Hypoxic preconditioning was shown to improve the therapeutic efficacy of bone marrow-derived multipotent mesenchymal stromal cells (MSCs) upon transplantation in ischemic tissue. Given the interest in clinical applications of umbilical cord blood-derived MSCs, we developed a specific hypoxic preconditioning protocol and investigated its anti-apoptotic and pro-angiogenic effects on cord blood MSCs undergoing simulated ischemia in vitro by subjecting them to hypoxia and nutrient deprivation with or without preceding hypoxic preconditioning...
2015: PloS One
https://www.readbyqxmd.com/read/26248379/in-vitro-and-in-vivo-effects-of-the-bumped-kinase-inhibitor-1294-in-the-related-cyst-forming-apicomplexans-toxoplasma-gondii-and-neospora-caninum
#14
Pablo Winzer, Joachim Müller, Adriana Aguado-Martínez, Mahbubur Rahman, Vreni Balmer, Vera Manser, Luis Miguel Ortega-Mora, Kayode K Ojo, Erkang Fan, Dustin J Maly, Wesley C Van Voorhis, Andrew Hemphill
We report on the in vitro effects of the bumped kinase inhibitor 1294 (BKI-1294) in cultures of virulent Neospora caninum isolates Nc-Liverpool (Nc-Liv) and Nc-Spain7 and in two strains of Toxoplasma gondii (RH and ME49), all grown in human foreskin fibroblasts. In these parasites, BKI-1294 acted with 50% inhibitory concentrations (IC50s) ranging from 20 nM (T. gondii RH) to 360 nM (N. caninum Nc-Liv), and exposure of intracellular stages to 1294 led to the nondisjunction of newly formed tachyzoites, resulting in the formation of multinucleated complexes similar to complexes previously observed in BKI-1294-treated N...
October 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/25912394/altered-mrna-levels-of-glucocorticoid-receptor-mineralocorticoid-receptor-and-co-chaperones-fkbp5-and-ptges3-in-the-middle-frontal-gyrus-of-autism-spectrum-disorder-subjects
#15
Neil Patel, Amanda Crider, Chirayu D Pandya, Anthony O Ahmed, Anilkumar Pillai
Although stress has been implicated in the pathophysiology of autistic spectrum disorder (ASD), it is not known whether glucocorticoid receptor (GR) levels are altered in the brain of subjects with ASD. The messenger RNA (mRNA) levels of GR isoforms (GRα, GRβ, GRγ, and GRP), mineralocorticoid receptor (MR), GR co-chaperones (FKBP5, PTGES3, and BAG1), and inflammatory cytokines (IL-6, IL-1β, and IFN-γ) were examined in the postmortem middle frontal gyrus tissues of 13 ASD and 13 age-matched controls by qRT-PCR...
May 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/25887985/in-vitro-cultivation-of-hammondia-heydorni-generation-of-tachyzoites-stage-conversion-into-bradyzoites-and-evaluation-of-serologic-cross-reaction-with-neospora-caninum
#16
L F P Gondim, J Meyer, M Peters, M M Rezende-Gondim, M G Vrhovec, N Pantchev, C Bauer, F J Conraths, G Schares
Hammondia heydorni was in vitro isolated from oocysts shed by three dogs using a finite cell line from embryonal bovine heart (KH-R). The oocysts were purified and suspended in 2% potassium dichromate or 2% sulphuric acid for sporulation for 2-5 days at room temperature. The parasites were confirmed as H. heydorni by PCR using specific primers (JS4/JS5) and by negative reaction for Neospora caninum employing the primers Np6+/Np21+. H. heydorni sporulated oocysts (1 × 10(6)) from each dog were initially treated with sodium hypochlorite...
June 15, 2015: Veterinary Parasitology
https://www.readbyqxmd.com/read/25807858/aav-mediated-expression-of-bag1-and-rock2-shrna-promote-neuronal-survival-and-axonal-sprouting-in-a-rat-model-of-rubrospinal-tract-injury
#17
Malleswari Challagundla, Jan Christoph Koch, Vinicius Toledo Ribas, Uwe Michel, Sebastian Kügler, Thomas Ostendorf, Frank Bradke, Hans Werner Müller, Mathias Bähr, Paul Lingor
A lesion to the rat rubrospinal tract is a model for traumatic spinal cord lesions and results in atrophy of the red nucleus neurons, axonal dieback, and locomotor deficits. In this study, we used adeno-associated virus (AAV)-mediated over-expression of BAG1 and ROCK2-shRNA in the red nucleus to trace [by co-expression of enhanced green fluorescent protein (EGFP)] and treat the rubrospinal tract after unilateral dorsal hemisection. We investigated the effects of targeted gene therapy on neuronal survival, axonal sprouting of the rubrospinal tract, and motor recovery 12 weeks after unilateral dorsal hemisection at Th8 in rats...
July 2015: Journal of Neurochemistry
https://www.readbyqxmd.com/read/25547791/reassessment-of-the-role-of-aromatic-amino-acid-hydroxylases-and-the-effect-of-infection-by-toxoplasma-gondii-on-host-dopamine
#18
Zi T Wang, Steve Harmon, Karen L O'Malley, L David Sibley
Toxoplasma gondii infection has been described previously to cause infected mice to lose their fear of cat urine. This behavioral manipulation has been proposed to involve alterations of host dopamine pathways due to parasite-encoded aromatic amino acid hydroxylases. Here, we report successful knockout and complementation of the aromatic amino acid hydroxylase AAH2 gene, with no observable phenotype in parasite growth or differentiation in vitro and in vivo. Additionally, expression levels of the two aromatic amino acid hydroxylases were negligible both in tachyzoites and in bradyzoites...
March 2015: Infection and Immunity
https://www.readbyqxmd.com/read/25407308/gene-expression-abnormalities-in-histologically-normal-breast-epithelium-from-patients-with-luminal-type-of-breast-cancer
#19
Pavol Zubor, Jozef Hatok, Petra Moricova, Karol Kajo, Ivana Kapustova, Andrea Mendelova, Peter Racay, Jan Danko
The gene expression profile of breast cancer has been described as a great breakthrough on the way to comprehend differences in cancer origin, behavior and therapy. However, gene expression profile in histologically normal epithelium (HNEpi) which could harbor genetic abnormalities predisposing breast tissue to develop malignancy was minor scope for scientists in the past. Thus, we aimed to analyze gene expressions in HNEpi and breast cancer tissue (BCTis) in order to establish its value as potential diagnostic marker for cancer development...
May 2015: Molecular Biology Reports
https://www.readbyqxmd.com/read/25300561/the-optimal-mixture-of-toxoplasma-gondii-recombinant-antigens-gra1-p22-rop1-for-diagnosis-of-ovine-toxoplasmosis
#20
Lucyna Holec-Gąsior, Bartłomiej Ferra, Elżbieta Hiszczyńska-Sawicka, Józef Kur
Toxoplasmosis, caused by Toxoplasma gondii, is the major parasitic disease affecting sheep. Infection not only results in significant reproductive losses in these animals, but has public health implications since consumption of infected meat can facilitate zoonotic transmission. Although several serological tests are currently used for diagnosis of ovine toxoplasmosis, production of reliable reagents is a constraint and therefore there is a need to develop new diagnostic tools. In this paper, we assess for the first time, the preliminary diagnostic utility of 19 T...
December 15, 2014: Veterinary Parasitology
keyword
keyword
52540
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"