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Lung drug deposition fluorescence imaging

Lina Wu, Xiaofei Wen, Xiance Wang, Chunan Wang, Xilin Sun, Kai Wang, Huiying Zhang, Todd Williams, Allen J Stacy, Junjie Chen, Anne H Schmieder, Gregory M Lanza, Baozhong Shen
Eighty percent of lung cancers originate as subtle premalignant changes in the airway mucosal epithelial layer of bronchi and alveoli, which evolve and penetrate deeper into the parenchyma. Liquid-ventilation, with perfluorocarbons (PFC) was first demonstrated in rodents in 1966 then subsequently applied as lipid-encapsulated PFC emulsions to improve pulmonary function in neonatal infants suffering with respiratory distress syndrome in 1996. Subsequently, PFC nanoparticles (NP) were extensively studied as intravenous (IV) vascular-constrained nanotechnologies for diagnostic imaging and targeted drug delivery applications...
2018: Theranostics
Aizat Turdalieva, Johan Solandt, Nestan Shambetova, Hao Xu, Hans Blom, Hjalmar Brismar, Marina Zelenina, Ying Fu
Lung epithelial cells are extensively exposed to nanoparticles present in the modern urban environment. Nanoparticles, including colloidal quantum dots (QDs), are also considered to be potentially useful carriers for the delivery of drugs into the body. It is therefore important to understand the ways of distribution and the effects of the various types of nanoparticles in the lung epithelium. We use a model system of liquid-covered human airway epithelial Calu-3 cell cultures to study the immediate and long-term effects of repeated deposition of colloidal 3-mercaptopropionic-acid coated CdSe-CdS/ZnS core-multishell QDs on the lung epithelial cell surface...
2016: PloS One
Shanka Walia, Supriya Sharma, Pankaj Markand Kulurkar, Vikram Patial, Amitabha Acharya
Multifunctional hybrid nanocomposite material, consists of chitosan encapsulated iron oxide (as MRI contrasting agent), CdS (as fluorescent probe) nanoparticles and podophyllotoxin (as anticancer drug) was synthesized and characterized. The TEM studies suggested the size of the NPs to be in the range of 80-100 nm. These nanocomposites were treated with different cancer cell lines viz., KB, C6 and A549 cells. Fluorescence imaging and Perl's Prussian blue staining confirmed the presence of these nanocomposites inside both KB and C6 cells but not in A549 cells...
February 10, 2016: International Journal of Pharmaceutics
Richard E Jacob, Wayne J Lamm, Daniel R Einstein, Melissa A Krueger, Robb W Glenny, Richard A Corley
PURPOSE: Computer models for inhalation toxicology and drug-aerosol delivery studies rely on ventilation pattern inputs for predictions of particle deposition and vapor uptake. However, changes in lung mechanics due to disease can impact airflow dynamics and model results. It has been demonstrated that non-invasive, in vivo, 4DCT imaging (3D imaging at multiple time points in the breathing cycle) can be used to map heterogeneities in ventilation patterns under healthy and disease conditions...
April 2015: Experimental Lung Research
Nathalie M Pinkerton, Stacey W Zhang, Richard L Youngblood, Dayuan Gao, Shike Li, Bryan R Benson, John Anthony, Howard A Stone, Patrick J Sinko, Robert K Prud'homme
The formation of 10-40 μm composite gel microparticles (CGMPs) comprised of ∼100 nm drug containing nanoparticles (NPs) in a poly(ethylene glycol) (PEG) gel matrix is described. The CGMP particles enable targeting to the lung by filtration from the venous circulation. UV radical polymerization and Michael addition polymerization reactions are compared as approaches to form the PEG matrix. A fluorescent dye in the solid core of the NP was used to investigate the effect of reaction chemistry on the integrity of encapsulated species...
January 13, 2014: Biomacromolecules
Shashank R Sirsi, Chinpong Fung, Sumit Garg, Mary Y Tianning, Paul A Mountford, Mark A Borden
The cavitation response of circulating microbubbles to targeted ultrasound can be used for noninvasive, site-specific delivery of shell-loaded materials. One challenge for microbubble-mediated delivery of lipophilic compounds is the limitation of drug loading into the microbubble shell, which is commonly a single phospholipid monolayer. In this study, we investigated the use of natural lung surfactant extract (Survanta(®), Abbott Nutrition) as a microbubble shell material in order to improve drug payload and delivery...
2013: Theranostics
Dandan Yi, Timothy Scott Wiedmann, Amir Naqwi, Andrew Patrick Price, Angela Panoskaltsis-Mortari
UNLABELLED: ABSTRACT Background: Obliterative bronchiolitis (OB) is a major obstacle to the success of lung transplantation and is also a serious complication of hematopoietic stem cell transplant. It has few therapeutic options and respiratory delivery of potential therapeutic drugs is hindered by the narrowed and occluded airways. METHODS: OB was induced in mice using an established protocol and lung function was assessed by plethysmograph. Mice were exposed to four different aerosols of aluminum phthalocyanine tetrasulfonic acid (AlPCS) that ranged in concentration and median particle size distribution (0...
September 2012: Experimental Lung Research
Dandan Yi, Amir Naqwi, Angela Panoskaltsis-Mortari, Timothy Scott Wiedmann
Better methods are needed to quantify the distribution of drug among the airways of the lungs of small animals to facilitate the development of agents that can target specific airways. Mice were exposed to aerosols of aluminum phthalocyanine tetrasulfonic acid (AlPCS) that ranged in concentration and size (0.2-2.8 μm). The trachea and lobes were removed and placed between glass slides, and fluorescent images were obtained at two different compression thicknesses. The intensity, normalized by the area, exposure time, and thickness, was then plotted as a function of compression thickness, from which the concentration and attenuation coefficient were estimated for each lobe and then for each pixel of the image...
April 15, 2012: International Journal of Pharmaceutics
Hak Soo Choi, Yoshitomo Ashitate, Jeong Heon Lee, Soon Hee Kim, Aya Matsui, Numpon Insin, Moungi G Bawendi, Manuela Semmler-Behnke, John V Frangioni, Akira Tsuda
Nano-size particles show promise for pulmonary drug delivery, yet their behavior after deposition in the lung remains poorly understood. In this study, a series of near-infrared (NIR) fluorescent nanoparticles were systematically varied in chemical composition, shape, size and surface charge, and their biodistribution and elimination were quantified in rat models after lung instillation. We demonstrate that nanoparticles with hydrodynamic diameter (HD) less than ≈34 nm and a noncationic surface charge translocate rapidly from the lung to mediastinal lymph nodes...
December 2010: Nature Biotechnology
Dandan Yi, Andrew Price, Angela Panoskaltsis-Mortari, Amir Naqwi, Timothy Scott Wiedmann
Lung samples were prepared to investigate the perturbing effects of light absorption for quantifying the fluorescence signal of aluminum phthalocyanine tetrasulfonic acid (AlPCS). Standard solutions of known concentration and depth were imaged with different exposure times and analyzed. The intensity was found to be a linear function of concentration, depth, exposure time, and area. Mice were exposed to an aerosol of AlPCS with a mass median aerodynamic diameter of 390 nm and geometric standard deviation of 1...
August 2010: Analytical Biochemistry
Mohammad A Yaseen, Jie Yu, Bongsu Jung, Michael S Wong, Bahman Anvari
Indocyanine green (ICG) is a fluorescent probe used in various optically mediated diagnostic and therapeutic applications. However, utility of ICG remains limited by its unstable optical properties and nonspecific localization. We have encapsulated ICG within electrostatically assembled mesocapsules (MCs) to explore its potential for targeted optical imaging and therapy. In this study, we investigate how the surface coating and size of the MCs influences ICG's biodistribution in vivo. ICG was administered intravenously to Swiss Webster mice as a free solution or encapsulated within either 100 nm diameter MCs coated with dextran; 500 nm diameter MCs coated with dextran; or 100 nm diameter MCs coated with 10 nm ferromagnetic iron oxide nanoparticles, themselves coated with polyethylene glycol...
September 2009: Molecular Pharmaceutics
Ching-Li Tseng, Steven Yueh-Hsiu Wu, Wen-Hsi Wang, Cheng-Liang Peng, Feng-Huei Lin, Chien-Cheng Lin, Tai-Horng Young, Ming-Jium Shieh
Lung cancer is the most malignant cancer today; in order to develop an effective drug delivery system for lung cancer therapy, gelatin nanoparticles (GPs) were modified with NeutrAvidin(FITC)-biotinylated epidermal growth factor (EGF) to form EGF receptor (EGFR)-seeking nanoparticles (GP-Av-bEGF). Aerosol droplets of the GP-Av-bEGF were generated by using a nebulizer and were delivered to mice model of lung cancer via aerosol delivery. Analysis of the aerosol size revealed that 99% of the nanoparticles after nebulization had a mass median aerodynamic diameter (MMAD) within the suitable range (0...
July 2008: Biomaterials
Bruno Tigani, Hans-Ulrich Gremlich, Catherine Cannet, Stefan Zurbruegg, Harry Karmouty Quintana, Nicolau Beckmann
PURPOSE: To address the issue concerning the predominant location, on the left anatomic side, of edematous signals detected by magnetic resonance imaging (MRI) in the lungs of actively sensitized rats following intratracheal (IT) allergen challenge. MATERIALS AND METHODS: Near-infrared fluorescence (NIRF) imaging was used to detect the lobular distribution in the lungs of normal rats of an IT instilled fluorescent dye, Cy5.5. Actively sensitized Brown Norway rats were examined by MRI 24 hours after IT administration of ovalbumin...
December 2004: Journal of Magnetic Resonance Imaging: JMRI
K Shimizu, Y Del Amo, M A Brzezinski, G D Stucky, D E Morse
BACKGROUND: Biological silica production has drawn intense attention and several molecules involved in biosilicification have been identified. Cellular mechanisms, however, remain unknown mainly due to the lack of probes required for obtaining information on live specimens. RESULTS: The fluorescence spectra of the compound 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) are affected by the presence of >3.2 mM silicic acid...
November 2001: Chemistry & Biology
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