keyword
https://read.qxmd.com/read/34828850/effect-of-different-winemaking-conditions-on-organic-acids-compounds-of-white-wines
#21
JOURNAL ARTICLE
Elena Cristina Scutarașu, Iulian Valentin Teliban, Cătălin Ioan Zamfir, Camelia Elena Luchian, Lucia Cintia Colibaba, Marius Niculaua, Valeriu V Cotea
Organic acids represent naturally occurring compounds that are found in many types of food and beverages, with important functions in defining products' final quality. Their proportions in wine are dependent on grape composition and winemaking conditions (temperature, pH levels, oxygen, and carbon dioxide concentration). Therefore, this article studied the influence of different fermentation conditions (200 hL tanks vs. 50 L glass demijohns) and various yeasts on the evolution of the main organic acids during alcoholic fermentation of "Aligoté" wines...
October 25, 2021: Foods (Basel, Switzerland)
https://read.qxmd.com/read/34778215/probing-multivalent-carbohydrate-protein-interactions-with-on-chip-synthesized-glycopeptides-using-different-functionalized-surfaces
#22
JOURNAL ARTICLE
Alexandra Tsouka, Kassandra Hoetzel, Marco Mende, Jasmin Heidepriem, Grigori Paris, Stephan Eickelmann, Peter H Seeberger, Bernd Lepenies, Felix F Loeffler
Multivalent ligand-protein interactions are a commonly employed approach by nature in many biological processes. Single glycan-protein interactions are often weak, but their affinity and specificity can be drastically enhanced by engaging multiple binding sites. Microarray technology allows for quick, parallel screening of such interactions. Yet, current glycan microarray methodologies usually neglect defined multivalent presentation. Our laser-based array technology allows for a flexible, cost-efficient, and rapid in situ chemical synthesis of peptide scaffolds directly on functionalized glass slides...
2021: Frontiers in Chemistry
https://read.qxmd.com/read/34686689/characterisation-of-factors-contributing-to-the-performance-of-nonwoven-fibrous-matrices-as-substrates-for-adenovirus-vectored-vaccine-stabilisation
#23
JOURNAL ARTICLE
Pawan Dulal, Robabeh Gharaei, Adam J Berg, Adam A Walters, Nicholas Hawkins, Tim D W Claridge, Katarzyna Kowal, Steven Neill, Adam J Ritchie, Rebecca Ashfield, Adrian V S Hill, Giuseppe Tronci, Stephen J Russell, Alexander D Douglas
Adenovirus vectors offer a platform technology for vaccine development. The value of the platform has been proven during the COVID-19 pandemic. Although good stability at 2-8 °C is an advantage of the platform, non-cold-chain distribution would have substantial advantages, in particular in low-income countries. We have previously reported a novel, potentially less expensive thermostabilisation approach using a combination of simple sugars and glass micro-fibrous matrix, achieving excellent recovery of adenovirus-vectored vaccines after storage at temperatures as high as 45 °C...
October 22, 2021: Scientific Reports
https://read.qxmd.com/read/34669384/photochemical-degradation-of-4-nitrocatechol-and-2-4-dinitrophenol-in-a-sugar-glass-secondary-organic-aerosol-surrogate
#24
JOURNAL ARTICLE
Avery B Dalton, Sergey A Nizkorodov
The roles that chemical environment and viscosity play in the photochemical fate of molecules trapped in atmospheric particles are poorly understood. The goal of this work was to characterize the photolysis of 4-nitrocatechol (4NC) and 2,4-dinitrophenol (24DNP) in semisolid isomalt as a new type of surrogate for glassy organic aerosols and compare it to photolysis in liquid water, isopropanol, and octanol. UV/vis spectroscopy was used to monitor the absorbance decay to determine the rates of photochemical loss of 4NC and 24DNP...
October 20, 2021: Environmental Science & Technology
https://read.qxmd.com/read/34351767/%C3%AE-hairpin-miniprotein-stabilization-in-trehalose-glass-is-facilitated-by-an-emergent-compact-non-native-state
#25
JOURNAL ARTICLE
Gil I Olgenblum, Frank Wien, Liel Sapir, Daniel Harries
From stem cell freeze-drying to organ storage, considerable recent efforts have been directed toward the development of new preservation technologies. A prominent protein stabilizing strategy involves vitrification in glassy matrices, most notably those formed of sugars such as the biologically relevant preservative trehalose. Here, we compare the folding thermodynamics of a model miniprotein in solution and in the glassy state of the sugars trehalose and glucose. Using synchrotron radiation circular dichroism (SRCD), we find that the same native structure persists in solution and glass...
August 5, 2021: Journal of Physical Chemistry Letters
https://read.qxmd.com/read/34316278/continuous-clarification-of-barberry-juice-with-pectinase-immobilised-by-oxidized-polysaccharides
#26
JOURNAL ARTICLE
Seyed Saeid Hosseini, Faramarz Khodaiyan, Seyed Mohammad Mousavi, Seyedeh Zahra Azimi
RESEARCH BACKGROUND: Barberry juice is a rich source of bioactive compounds and shows different health properties such as antioxidant and anticancer activities. Clarification, as the removal process of suspended material, is an important step in the production of fruit juice due to its significant effect on the appearance, flavour and commercialisation of juice. Pectinase is the most important enzyme applied in juice clarification that breaks down the pectin polymer structure and reduces the undesirable turbidity...
June 2021: Food Technology and Biotechnology
https://read.qxmd.com/read/33744450/stabilization-mechanism-of-amorphous-carbamazepine-by-transglycosylated-rutin-a-non-polymeric-amorphous-additive-with-a-high-glass-transition-temperature
#27
JOURNAL ARTICLE
Chisa Aoki, Xiaohan Ma, Kenjirou Higashi, Yuya Ishizuka, Keisuke Ueda, Kazunori Kadota, Kaori Fukuzawa, Yuichi Tozuka, Kohsaku Kawakami, Etsuo Yonemochi, Kunikazu Moribe
α-Glycosyl rutin (Rutin-G), composed of a flavonol skeleton and sugar groups, is a promising non-polymeric additive for stabilizing amorphous drug formulations. In this study, the mechanism of the stabilization of the amorphous state of carbamazepine (CBZ) by Rutin-G was investigated. In comparison with hypromellose (HPMC), which is commonly used as a crystallization inhibitor for amorphous drugs, Rutin-G significantly stabilized amorphous CBZ. Moreover, the dissolution rate and the resultant supersaturation level of CBZ were significantly improved in the CBZ/Rutin-G spray-dried samples (SPDs) owing to the rapid dissolution property of Rutin-G...
March 17, 2021: International Journal of Pharmaceutics
https://read.qxmd.com/read/33290511/effect-of-commercial-protective-cultures-and-bacterial-fermentates-on-listeria-monocytogenes-growth-in-a-refrigerated-high-moisture-model-cheese
#28
JOURNAL ARTICLE
Sarah K Engstrom, Kory M Anderson, Kathleen Ann Glass
Biopreservatives are clean-label ingredients used to control pathogenic and spoilage microorganisms in ready-to-eat foods including cheese. The efficacies of six commercial biopreservatives in controlling L. monocytogenes growth at 4°C were tested in a high-moisture model cheese (pH 6.00, 56% moisture, 1.25% salt) made of cream, micellar casein, water, salt, lactose, lactic acid, and a single protective culture (PC-1, PC-2, or PC-3, 10 6 CFU/g target) or bacterial fermentate (CM-1 or CM-2 [cultured milk] or CSV-1 [cultured sugar-vinegar blend], 0...
December 8, 2020: Journal of Food Protection
https://read.qxmd.com/read/33200971/first-report-of-alternaria-tenuissima-causing-leaf-blight-on-sugarcane-in-china
#29
JOURNAL ARTICLE
Jie Li, Rong-Yue Zhang, Xiao-Yan Wang, Hong-Li Shan, Chang-Mi Wang, Xiao-Yan Cang, Ying-Kun Huang
Sugarcane (Saccharum officinarum L.) is the main sugar crop in China. Yunnan is the second largest sugarcane production province in China. In December 2018, leaf blight was first observed on almost every leaf of sugarcane on 'Huanan 54-11', 'Baimei' and 'Chongan' in Kaiyuan (103°27' E, 23°72' N), Yunnan. In October 2019, during our survey in the field in Lingcang (100°08' E, 23°88' N), Yunnan, this disease was also observed on 'ROC 25'. Symptoms of the disease initially appeared as wilted, which seemed to be cause by water stress...
November 17, 2020: Plant Disease
https://read.qxmd.com/read/31593692/me-2-so-and-serum-free-cryopreservation-of-human-umbilical-cord-mesenchymal-stem-cells-using-electroporation-assisted-delivery-of-sugars
#30
JOURNAL ARTICLE
Vitalii Mutsenko, Ariana Barlič, Tamara Pezić, Janja Dermol-Černe, Barbara Dovgan, Bulat Sydykov, Willem F Wolkers, Igor I Katkov, Birgit Glasmacher, Damijan Miklavčič, Oleksandr Gryshkov
Cryopreservation is the universal technology used to enable long-term storage and continuous availability of cell stocks and tissues for regenerative medicine demands. The main components of standard freezing media are dimethyl sulfoxide (hereinafter Me2 SO) and fetal bovine serum (FBS). However, for manufacturing of cells and tissue-engineered products in accordance with the principles of Good Manufacturing Practice (GMP), current considerations in regenerative medicine suggest development of Me2 SO- and serum-free biopreservation strategies due to safety concerns over Me2 SO-induced side effects and immunogenicity of animal serum...
October 5, 2019: Cryobiology
https://read.qxmd.com/read/31349031/thermostable-lyoprotectant-enhanced-cell-free-protein-synthesis-for-on-demand-endotoxin-free-therapeutic-production
#31
JOURNAL ARTICLE
Kristen M Wilding, Emily Long Zhao, Conner C Earl, Bradley C Bundy
Cell-free protein synthesis has emerged as a promising platform for the production of therapeutic proteins due to its inherently open reaction environment, flexible reaction conditions and rapid protein synthesis capabilities. In recent years, lyophilized cell-free systems have widened the application space of cell-free technology by improving reagent stability outside of cold-chain storage. Current embodiments of the system, however, demonstrate poor stability at elevated temperatures. Lyoprotectants have long been recognized for the ability to preserve the activity of biological molecules during drying processes, but the application of this technology to lyophilized cell-free systems has been limited and has failed to address the negative effects that such lyoprotectants may have on cell-free systems...
July 23, 2019: New Biotechnology
https://read.qxmd.com/read/31306751/a-spray-freeze-dried-micropellet-based-formulation-proof-of-concept-for-a-yellow-fever-vaccine-candidate
#32
JOURNAL ARTICLE
Didier Clénet, Véronique Hourquet, Bertrand Woinet, Hervé Ponceblanc, Manuel Vangelisti
The stability of live-attenuated viruses is very challenging due to thermal sensitivity; therefore, solid form is usually required (often freeze-dried products). Micropellet technology is a lyophilization technology that has the potential to provide greater flexibility in the presentation of a given vaccine particularly in multi-dose format or in combination of different vaccines. As a novel vaccine alternative process, this spray freeze-dried (SFD) micropellet technology was evaluated using as a model a yellow fever virus produced in Vero cells (vYF)...
July 12, 2019: European Journal of Pharmaceutics and Biopharmaceutics
https://read.qxmd.com/read/29568011/inhibitory-effect-of-surface-pre-reacted-glass-ionomer-s-prg-eluate-against-adhesion-and-colonization-by-streptococcus-mutans
#33
JOURNAL ARTICLE
Ryota Nomura, Yumiko Morita, Saaya Matayoshi, Kazuhiko Nakano
Surface Pre-reacted Glass-ionomer (S-PRG) filler is a bioactive filler produced by PRG technology, which has been applied to various dental materials. A S-PRG filler can release multiple ions from a glass-ionomer phase formed in the filler. In the present study, detailed inhibitory effects induced by S-PRG eluate (prepared with S-PRG filler) against Streptococcus mutans, a major pathogen of dental caries, were investigated. S-PRG eluate effectively inhibited S. mutans growth especially in the bacterium before the logarithmic growth phase...
March 22, 2018: Scientific Reports
https://read.qxmd.com/read/29143888/-the-art-of-insulin-treatment-diabetes-insulin-and-the-1920s
#34
JOURNAL ARTICLE
Kirsten E Gardner
Soon after the discovery of insulin in the early 1920s, the popular press celebrated the miraculous discovery. Although insulin had no curative effect on the chronic state of diabetes, it was frequently heralded as a "cure." This paper examines how the discovery of insulin intersected with the rise of diabetic technology and the transfer of medical technology to the home setting. By analyzing diabetic manuals written for patients and physicians, letters exchanged between patient and physician, medical journals, magazines and newspapers, I trace how patients learned about insulin and more significantly how patients adopted measurement technologies designed to allow better home administration of insulin...
November 16, 2017: Journal of Medical Humanities
https://read.qxmd.com/read/26936658/mode-of-action-of-cryoprotectants-for-sperm-preservation
#35
REVIEW
Harald Sieme, Harriëtte Oldenhof, Willem F Wolkers
Sperm cryopreservation facilitates storage and transport for use in artificial reproduction technologies. Cryopreservation processing, however, exposes cells to stress resulting in cellular damage compromising sperm function. Cryoprotective agents are needed to minimize cryopreservation injury, but at higher concentration they are toxic to cells. In this review, we describe cryoinjury mechanisms, and modes of action of different types of cryoprotective agents. Furthermore, measures are discussed how to minimize toxic effects caused by adding and removing cryoprotective agents...
June 2016: Animal Reproduction Science
https://read.qxmd.com/read/25702912/the-peculiar-behavior-of-the-glass-transition-temperature-of-amorphous-drug-polymer-films-coated-on-inert-sugar-spheres
#36
JOURNAL ARTICLE
Aswin Dereymaker, Guy Van Den Mooter
Fluid bed coating has been proposed in the past as an alternative technology for manufacturing of drug-polymer amorphous solid dispersions, or so-called glass solutions. It has the advantage of being a one-step process, and thus omitting separate drying steps, addition of excipients, or manipulation of the dosage form. In search of an adequate sample preparation method for modulated differential scanning calorimetry analysis of beads coated with glass solutions, glass transition broadening and decrease of the glass transition temperature (Tg ) were observed with increasing particle size of crushed coated beads and crushed isolated films of indomethacin (INDO) and polyvinylpyrrolidone (PVP)...
May 2015: Journal of Pharmaceutical Sciences
https://read.qxmd.com/read/25157000/smart-phone-obesity-prevention-trial-for-adolescent-boys-in-low-income-communities-the-atlas-rct
#37
RANDOMIZED CONTROLLED TRIAL
Jordan J Smith, Philip J Morgan, Ronald C Plotnikoff, Kerry A Dally, Jo Salmon, Anthony D Okely, Tara L Finn, David R Lubans
OBJECTIVE: The goal of this study was to evaluate the impact of the Active Teen Leaders Avoiding Screen-time (ATLAS) intervention for adolescent boys, an obesity prevention intervention using smartphone technology. METHODS: ATLAS was a cluster randomized controlled trial conducted in 14 secondary schools in low-income communities in New South Wales, Australia. Participants were 361 adolescent boys (aged 12-14 years) considered at risk of obesity. The 20-week intervention was guided by self-determination theory and social cognitive theory and involved: teacher professional development, provision of fitness equipment to schools, face-to-face physical activity sessions, lunchtime student mentoring sessions, researcher-led seminars, a smartphone application and Web site, and parental strategies for reducing screen-time...
September 2014: Pediatrics
https://read.qxmd.com/read/24966423/reduction-of-non-enzymatic-browning-of-orange-juice-and-semi-concentrates-by-removal-of-reaction-substrate
#38
JOURNAL ARTICLE
Satish K Sharma, Shashibala Juyal, V K Rao, V K Yadav, A K Dixit
A study was conducted to standardize the technology for the removal of amino acids (one of the browning reaction substrates) from sweet orange cv. Malta Common juice to reduce colour and quality deterioration in single strength juice and during subsequent concentration. Juice of sweet orange (Citrus sinensis) cv. Malta Common fruits was extracted by screw type juice extractor, preserved in 500 ppm SO2 and clarified by using "Pectinase CCM" enzyme (0.2% for 2 h at 50 ± 2 °C). For removal of amino acids juice was passed under gravity through a glass column packed with an acidic cation exchange resin (CER), Dowex-50 W and quantity to be treated in one lot was standardized...
July 2014: Journal of Food Science and Technology
https://read.qxmd.com/read/24964165/sugar-mediated-disassembly-of-mucin-lectin-multilayers-and-their-use-as-ph-tolerant-on-demand-sacrificial-layers
#39
JOURNAL ARTICLE
Roberta Polak, Thomas Crouzier, Rosanna M Lim, Katharina Ribbeck, Marisa M Beppu, Ronaldo N M Pitombo, Robert E Cohen, Michael F Rubner
The layer-by-layer (LbL) assembly of thin films on surfaces has proven to be an extremely useful technology for uses ranging from optics to biomedical applications. Releasing these films from the substrate to generate so-called free-standing multilayer films opens a new set of applications. Current approaches to generating such materials are limited because they can be cytotoxic, difficult to scale up, or have undesirable side reactions on the material. In this work, a new sacrificial thin film system capable of chemically triggered dissolution at physiological pH of 7...
August 11, 2014: Biomacromolecules
https://read.qxmd.com/read/23872303/detecting-phase-separation-of-freeze-dried-binary-amorphous-systems-using-pair-wise-distribution-function-and-multivariate-data-analysis
#40
JOURNAL ARTICLE
Norman Chieng, Hjalte Trnka, Johan Boetker, Michael Pikal, Jukka Rantanen, Holger Grohganz
The purpose of this study is to investigate the use of multivariate data analysis for powder X-ray diffraction-pair-wise distribution function (PXRD-PDF) data to detect phase separation in freeze-dried binary amorphous systems. Polymer-polymer and polymer-sugar binary systems at various ratios were freeze-dried. All samples were analyzed by PXRD, transformed to PDF and analyzed by principal component analysis (PCA). These results were validated by differential scanning calorimetry (DSC) through characterization of glass transition of the maximally freeze-concentrate solute (Tg')...
September 15, 2013: International Journal of Pharmaceutics
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