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Pulmonary fluorescence imaging

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https://www.readbyqxmd.com/read/28798254/oxygen-dependent-changes-in-lung-development-do-not-affect-epithelial-infection-with-influenza-a-virus
#1
William Domm, Min Yee, Ravi S Misra, Robert Gelein, Aitor Nogales, Luis Martinez Sobrido, Michael A O'Reilly
Infants born prematurely often require supplemental oxygen that contributes to aberrant lung development and increased pulmonary morbidity following a respiratory viral infection. We have been using a mouse model to understand how early-life hyperoxia affects the adult lung response to influenza A virus (IAV) infection. Prior studies showed how neonatal hyperoxia (100% oxygen) increased sensitivity of adult mice to infection with influenza A virus (IAV (A/Hong Kong/X31) H3N2) as defined by persistent inflammation, pulmonary fibrosis, and mortality...
August 10, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28796342/elucidation-of-insulin-assembly-at-acidic-and-neutral-ph-characterization-of-low-molecular-weight-oligomers
#2
Matthew T Mawhinney, Thomas L Williams, James L Hart, Mitra L Taheri, Brigita Urbanc
Deficiency in insulin secretion and function that characterize type 2 diabetes often requires administration of extraneous insulin, leading to injection-site amyloidosis. Insulin aggregation at neutral pH is not well understood. Although oligomer formation is believed to play an important role, insulin oligomers have not been fully characterized yet. Here, we elucidate similarities and differences between in vitro insulin aggregation at acidic and neutral pH for a range of insulin concentrations (2.5-100 μM) by using kinetic thioflavin T fluorescence, circular dichroism, atomic force and electron microscopy imaging...
August 10, 2017: Proteins
https://www.readbyqxmd.com/read/28746152/intraoperative-molecular-imaging-combined-with-positron-emission-tomography-improves-surgical-management-of-peripheral-malignant-pulmonary-nodules
#3
Jarrod D Predina, Andrew D Newton, Jane Keating, Eduardo M Barbosa, Olugbenga Okusanya, Leilei Xia, Ashley Dunbar, Courtney Connolly, Michael P Baldassari, Jack Mizelle, Edward J Delikatny, John C Kucharczuk, Charuhas Deshpande, Sumith A Kularatne, Phillip Low, Jeffrey Drebin, Sunil Singhal
OBJECTIVE: To determine if intraoperative molecular imaging (IMI) can improve detection of malignant pulmonary nodules. BACKGROUND: 18-Fluorodeoxyglucose positron emission tomography (PET) is commonly utilized in preoperative assessment of patients with solid malignancies; however, false negatives and false positives remain major limitations. Using patients with pulmonary nodules as a study model, we hypothesized that IMI with a folate receptor targeted near-infrared contrast agent (OTL38) can improve malignant pulmonary nodule identification when combined with PET...
September 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28641278/astragaloside-iv-alleviates-heat-induced-inflammation-by-inhibiting-endoplasmic-reticulum-stress-and-autophagy
#4
Zhiwei Dong, Jian Zhou, Ying Zhang, Yajie Chen, Zichen Yang, Guangtao Huang, Yu Chen, Zhiqiang Yuan, Yizhi Peng, Tongtong Cao
BACKGROUND: Thermal injury is the main cause of pulmonary disease in stroke after burn and can be life threatening. Heat-induced inflammation is an important factor that triggers a series of induces pathological changes. However, this mechanism underlying heat-induced inflammation in thermal inhalation injury remains unclear. Studies have revealed that astragaloside-IV (AS-IV), a natural compound extracted from Astragalus membranaceus, has protective effects in inflammatory diseases. Here, we investigated whether the protective effects of AS-IV occur because of the suppression of heat-induced endoplasmic reticulum (ER) stress and excessive autophagy Methods: AS-IV was administered to Wistar rats after thermal inhalation injury and 16HBE140-cells were treated with AS-IV...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28569457/advances-in-radioguided-surgery-in-oncology
#5
Renato A Valdés Olmos, Sergi Vidal-Sicart, Gianpiero Manca, Giuliano Mariani, Luisa F León-Ramírez, Domenico Rubello, Francesco Giammarile
The sentinel lymph node (SLN) biopsy is probably the most well known radioguided technique in surgical oncology. Today SLN biopsy reduces the morbidity associated with lymphadenectomy and increases the identification rate of occult lymphatic metastases by offering the pathologist the lymph nodes with the highest probability of containing metastatic cells. These advantages may result in a change in clinical clinical management both in melanoma and breast cancer patients. The SLN evaluation by pathology currently implies tumour burden stratification for further prognostic information...
June 1, 2017: Quarterly Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28536424/a-microfluidic-chamber-based-approach-to-map-the-shear-moduli-of-vascular-cells-and-other-soft-materials
#6
Béla Suki, Yingying Hu, Naohiko Murata, Jasmin Imsirovic, Jarred R Mondoñedo, Claudio L N de Oliveira, Niccole Schaible, Philip G Allen, Ramaswamy Krishnan, Erzsébet Bartolák-Suki
There is growing interest in quantifying vascular cell and tissue stiffness. Most measurement approaches, however, are incapable of assessing stiffness in the presence of physiological flows. We developed a microfluidic approach which allows measurement of shear modulus (G) during flow. The design included a chamber with glass windows allowing imaging with upright or inverted microscopes. Flow was controlled gravitationally to push culture media through the chamber. Fluorescent beads were conjugated to the sample surface and imaged before and during flow...
May 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28498283/synovial-sarcoma-of-peripheral-nerves-analysis-of-15-cases
#7
John S A Chrisinger, Usama I Salem, Lars-Gunnar Kindblom, Behrang Amini, Magnus Hansson, Jeanne M Meis
Synovial sarcoma of peripheral nerve (SSPN) is rare with only 26 cases reported in English. SSPN is often mistaken for a benign or malignant peripheral nerve sheath tumor (PNST) by clinicians and pathologists. Fifteen cases of SSPN were retrieved from the pathology files of 3 institutions. All tumors arose in a nerve based on imaging and/or operative findings and the diagnoses were histologically confirmed. Neuropathic symptoms predominated in 11 women and 4 men, 19- to 62-year-old (median, 39 years) with tumors involving the ulnar (5), median (3), peroneal (3) or sciatic (2) nerve, or the L4 or T1 nerve root (2)...
August 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28497233/standardization-and-optimization-of-intraoperative-molecular-imaging-for-identifying-primary-pulmonary-adenocarcinomas
#8
Jarrod D Predina, Olugbenga Okusanya, Andrew D Newton, Philip Low, Sunil Singhal
PURPOSE: Intraoperative molecular imaging (IMI) is an emerging technology used to locate pulmonary adenocarcinomas and identify positive margins during surgery. Background noise and tissue autofluorescence have been major obstacles. The goal of this study is to optimize the image quality of folate receptor alpha (FRα) targeted IMI for pulmonary adenocarcinomas by modifying emission data. PROCEDURES: A total of 15 lung cancer patients were enrolled in a pilot study...
May 11, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28495056/a-novel-minimally-invasive-near-infrared-thoracoscopic-localization-technique-of-small-pulmonary-nodules-a-phase-i-feasibility-trial
#9
Hideki Ujiie, Tatsuya Kato, Hsin-Pei Hu, Priya Patel, Hironobu Wada, Kosuke Fujino, Robert Weersink, Elsie Nguyen, Marcelo Cypel, Andrew Pierre, Marc de Perrot, Gail Darling, Thomas K Waddell, Shaf Keshavjee, Kazuhiro Yasufuku
OBJECTIVES: Localization and resection of nonvisible, nonpalpable pulmonary nodules during video-assisted thoracoscopic surgery are challenging. Our study was to determine the feasibility and safety of indocyanine green fluorescence localization and resection of small nodules using a near-infrared fluorescence thoracoscope. METHODS: Twenty patients with undiagnosed peripheral nodules smaller than 3 cm scheduled for computed tomography-guided microcoil placement followed by video-assisted thoracoscopic surgery wedge resection were enrolled...
August 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28435447/a-unique-recombinant-fluoroprobe-targeting-activated-platelets-allows-in-vivo-detection-of-arterial-thrombosis-and-pulmonary-embolism-using-a-novel-three-dimensional-fluorescence-emission-computed-tomography-flect-technology
#10
Bock Lim, Yu Yao, Alex Lin-I Huang, May Lin Yap, Ulrike Flierl, Jathushan Palasubramaniam, Maria T K Zaldivia, Xiaowei Wang, Karlheinz Peter
Progress in pharmaceutical development is highly-dependent on preclinical in vivo animal studies. Small animal imaging is invaluable for the identification of new disease markers and the evaluation of drug efficacy. Here, we report for the first time the use of a three-dimensional fluorescence bioimager called FLuorescence Emission Computed Tomography (FLECT) for the detection of a novel recombinant fluoroprobe that is safe, easily prepared on a large scale and stably stored prior to scan. This novel fluoroprobe (Targ-Cy7) comprises a single-chain antibody-fragment (scFvTarg), which binds exclusively to activated-platelets, conjugated to a near-infrared (NIR) dye, Cy7, for detection...
2017: Theranostics
https://www.readbyqxmd.com/read/28419866/o-aminobenzoyl-s-nitrosoglutathione-a-fluorogenic-cell-permeable-pseudo-substrate-for-s-nitrosoglutathione-reductase
#11
Bei Lei Sun, Lisa Palmer, Shagufta Rehman Alam, Itunuoluwa Adekoya, Kathleen Brown-Steinke, Ammasi Periasamy, Bulent Mutus
S-nitrosoglutathione reductase (GSNOR) is a multifunctional enzyme. It can catalyze NADH-dependent reduction of S-nitrosoglutathione (GSNO); as well as NAD(+)-dependent oxidation of hydroxymethylglutathione (HMGSH; an adduct formed by the spontaneous reaction between formaldehyde and glutathione). While initially recognized as the enzyme that is involved in formaldehyde detoxification, increasing amount of evidence has shown that GSNOR also plays a significant role in nitric oxide mediated signaling through its modulation of protein S-nitrosothiol signaling...
April 15, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28416105/do-clear-cell-papillary-renal-cell-carcinomas-have-malignant-potential-diolombi-ml-cheng-l-argani-p-epstein-ji-am-j-surg-pathol-december-2015-39-12-1621-1634
#12
Oleksandr N Kryvenko
There have been no recurrences or metastases of clear cell papillary renal cell carcinoma (CCPRCC) in 268 reported cases with follow-up in the English-language literature. We identified all our cases of CCPRCC (1990-2013), reviewing all cases that preceded the formal designation of the entity. Immunohistochemical stains were performed on 32 cases during their initial workup. In addition, stains for carbonic anhydrase IX and cytokeratin 7 were performed on 2 cases, one with atypical follow-up and the other with a more compact morphology, although not performed initially...
June 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28392314/persistence-and-proliferation-of-human-mesenchymal-stromal-cells-in-the-right-ventricular-myocardium-after-intracoronary-injection-in-a-large-animal-model-of-pulmonary-hypertension
#13
Roza Badr Eslam, Kevin Croce, Fernanda Marinho Mangione, Robert Musmann, Jane A Leopold, Richard N Mitchell, Aaron B Waxman
BACKGROUND AIMS: In this study, we demonstrate long-term persistence of human mesenchymal stromal cells (hMSCs) after intracoronary injection in a large animal model of pulmonary hypertension (PH). METHODS: Commercially available placenta-derived hMSCs were used. Experiments were conducted on 14 female Yorkshire swine. Four animals served as controls, and 10 underwent pulmonary vein (PV) banding. After 12 ± 2 weeks, PH and PV dysfunction were confirmed by right heart catheterization and cardiac magnetic resonance imaging...
June 2017: Cytotherapy
https://www.readbyqxmd.com/read/28373627/effects-of-transplanted-human-cord-blood-mononuclear-cells-on-pulmonary-hypertension-in-immunodeficient-mice-and-their-distribution
#14
Mikio Sugano, Homare Yoshida, Hirotsugu Kurobe, Hiroki Arase, Hajime Kinoshita, Takashi Kitaichi, Noriko Sugasawa, Soichiro Nakayama, Kazuhisa Maeda, Minoru Irahara, Tetsuya Kitagawa
OBJECTIVES: To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. METHODS: MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation...
2017: Journal of Medical Investigation: JMI
https://www.readbyqxmd.com/read/28298912/heterologous-matrix-metalloproteinase-gene-promoter-activity-allows-in-vivo-real-time-imaging-of-bleomycin-induced-lung-fibrosis-in-transiently-transgenized-mice
#15
Fabio Franco Stellari, Francesca Ruscitti, Daniela Pompilio, Francesca Ravanetti, Giulia Tebaldi, Francesca Macchi, Andrea Elizabeth Verna, Gino Villetti, Gaetano Donofrio
Idiopathic pulmonary fibrosis is a very common interstitial lung disease derived from chronic inflammatory insults, characterized by massive scar tissue deposition that causes the progressive loss of lung function and subsequent death for respiratory failure. Bleomycin is used as the standard agent to induce experimental pulmonary fibrosis in animal models for the study of its pathogenesis. However, to visualize the establishment of lung fibrosis after treatment, the animal sacrifice is necessary. Thus, the aim of this study was to avoid this limitation by using an innovative approach based on a double bleomycin treatment protocol, along with the in vivo images analysis of bleomycin-treated mice...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28269438/a-novel-wireless-wearable-fluorescence-image-guided-surgery-system
#16
Kunshan He, Yamin Mao, Jinzuo Ye, Yu An, Shixin Jiang, Chongwei Chi, Jie Tian
Segmentectomy using indocyanine green (ICG) has become a primary treatment option to achieve a complete resection and preserve lung function in early-stage lung cancer. However, owing to a lack of appropriate intraoperative imaging systems, it is a huge challenge for surgeons to identify the intersegmental plane during the operation, leading to poor prognosis. Thus, we developed a novel wireless wearable fluorescence image-guided surgery system (LIGHTEN) for fast and accurate identification of intersegmental planes in human patients...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28263385/intraoperative-near-infrared-fluorescence-imaging-targeting-folate-receptors-identifies-lung-cancer-in-a-large-animal-model
#17
Jane J Keating, Jeffrey J Runge, Sunil Singhal, Sarah Nims, Ollin Venegas, Amy C Durham, Gary Swain, Shuming Nie, Philip S Low, David E Holt
BACKGROUND: Complete tumor resection is the most important predictor of patient survival with non-small cell lung cancer. Methods for intraoperative margin assessment after lung cancer excision are lacking. This study evaluated near-infrared (NIR) intraoperative imaging with a folate-targeted molecular contrast agent (OTL0038) for the localization of primary lung adenocarcinomas, lymph node sampling, and margin assessment. METHODS: Ten dogs with lung cancer underwent either video-assisted thoracoscopic surgery or open thoracotomy and tumor excision after an intravenous injection of OTL0038...
May 15, 2017: Cancer
https://www.readbyqxmd.com/read/28227684/a-novel-wireless-wearable-fluorescence-image-guided-surgery-system
#18
Kunshan He, Yamin Mao, Jinzuo Ye, Yu An, Shixin Jiang, Chongwei Chi, Jie Tian, Kunshan He, Yamin Mao, Jinzuo Ye, Yu An, Shixin Jiang, Chongwei Chi, Jie Tian, Shixin Jiang, Jinzuo Ye, Kunshan He, Chongwei Chi, Jie Tian, Yamin Mao, Yu An
Segmentectomy using indocyanine green (ICG) has become a primary treatment option to achieve a complete resection and preserve lung function in early-stage lung cancer. However, owing to a lack of appropriate intraoperative imaging systems, it is a huge challenge for surgeons to identify the intersegmental plane during the operation, leading to poor prognosis. Thus, we developed a novel wireless wearable fluorescence image-guided surgery system (LIGHTEN) for fast and accurate identification of intersegmental planes in human patients...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28213277/fluorescence-and-computed-tomography-for-assessing-the-biodistribution-of-sirna-after-intratracheal-application-in-mice
#19
Antonia Geyer, Cornelia Lorenzer, Sebastian Gehrig, Manuela Simlinger, Johannes Winkler, Haider Sami, Manfred Ogris
Pulmonary delivery of nucleic acids opens the possibility for direct treatment of lung diseases, like fibrosis, cancer, and infections. Lung retention and biodistribution of nucleic acids remain important issues for the development of suitable therapeutic approaches. Moreover, monitoring the dynamic biodistribution processes of siRNA after aerosol delivery can help in identifying bottlenecks and optimizing therapeutic concepts. We investigated dynamic biodistribution events after intratracheal application of chemically stabilized siRNA labelled with near infrared emitting dye AlexaFluor750 (AF750)...
February 14, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28176769/heterogeneity-of-macrophage-infiltration-and-therapeutic-response-in-lung-carcinoma-revealed-by-3d-organ-imaging
#20
Michael F Cuccarese, J Matthew Dubach, Christina Pfirschke, Camilla Engblom, Christopher Garris, Miles A Miller, Mikael J Pittet, Ralph Weissleder
Involvement of the immune system in tumour progression is at the forefront of cancer research. Analysis of the tumour immune microenvironment has yielded a wealth of information on tumour biology, and alterations in some immune subtypes, such as tumour-associated macrophages (TAM), can be strong prognostic indicators. Here, we use optical tissue clearing and a TAM-targeting injectable fluorescent nanoparticle (NP) to examine three-dimensional TAM composition, tumour-to-tumour heterogeneity, response to colony-stimulating factor 1 receptor (CSF-1R) blockade and nanoparticle-based drug delivery in murine pulmonary carcinoma...
February 8, 2017: Nature Communications
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