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https://www.readbyqxmd.com/read/28729420/revisiting-the-substrate-specificity-of-mammalian-%C3%AE-1-6-fucosyltransferase-fut8-reveals-that-it-catalyzes-core-fucosylation-of-n-glycans-lacking-%C3%AE-1-3-arm-glcnac
#1
Qiang Yang, Roushu Zhang, Hui Cai, Lai-Xi Wang
The mammalian α1,6-fucosyltransferase (FUT8) catalyzes the core fucosylation of N-glycans in the biosynthesis of glycoproteins. Previously, intensive in vitro studies with crude extract or purified enzyme concluded that the attachment of a GlcNAc on the α1,3 mannose arm of N-glycan is essential for FUT8 catalyzed core fucosylation. In contrast, we have recently shown that expression of erythropoietin (EPO) in a GnTI-knockout, FUT8 overexpressing cell line results in the production of fully core-fucosylated glycoforms of the oligomannose substrate Man5GlcNAc2, suggesting that FUT8 can catalyze core fucosylation of N-glycans lacking an α1,3arm GlcNAc in cells...
July 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28725337/complete-genome-sequence-of-leuconostoc-suionicum-dsm-20241-t-provides-insights-into-its-functional-and-metabolic-features
#2
Byung Hee Chun, Se Hee Lee, Hye Hee Jeon, Dong-Woon Kim, Che Ok Jeon
The genome of Leuconostoc suionicum DSM 20241(T) (=ATCC 9135(T) = LMG 8159(T) = NCIMB 6992(T)) was completely sequenced and its fermentative metabolic pathways were reconstructed to investigate the fermentative properties and metabolites of strain DSM 20241(T) during fermentation. The genome of L. suionicum DSM 20241(T) consists of a circular chromosome (2026.8 Kb) and a circular plasmid (21.9 Kb) with 37.58% G + C content, encoding 997 proteins, 12 rRNAs, and 72 tRNAs. Analysis of the metabolic pathways of L...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28724630/altered-met-receptor-phosphorylation-and-lrp1-mediated-uptake-in-cells-lacking-carbohydrate-dependent-lysosomal-targeting
#3
Megan Aarnio-Peterson, Peng Zhao, Seok-Ho Yu, Courtney Christian, Heather Flanagan-Steet, Lance Wells, Richard Steet
Acid hydrolases utilize a carbohydrate-dependent mechanism for lysosomal targeting. These hydrolases acquire a mannose 6-phosphate tag by the action of the GlcNAc-1-phosphotransferase enzyme, allowing them to bind receptors and traffic to endosomes. Loss of GlcNAc-1-phosphotransferase results in hydrolase hypersecretion and profound lysosomal storage. Little, however, is known about how these cellular phenotypes affect the trafficking, activity and localization of surface glycoproteins. To address this question, we profiled the abundance of surface glycoproteins in WT and CRISPR-mediated GNPTAB-/- HeLa cells and identified changes in numerous glycoproteins including the uptake receptor LRP1 and multiple receptor tyrosine kinases...
July 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28723213/dual-functional-core-shell-fluorescent-ag2s-carbon-nanostructure-for-selective-assay-of-e-coli-o157-h7-and-bactericidal-treatment
#4
Ning Wang, Xing Wei, An-Qi Zheng, Ting Yang, Ming-Li Chen, Jian-Hua Wang
A dual functional fluorescent core-shell Ag2S@Carbon nanostructure is prepared by a hydrothermally assisted multi-amino synthesis approach with folic acid (FA), polyethylenimine (PEI), and mannoses (Mans) as carbon and nitrogen sources (FA-PEI-Mans-Ag2S nanocomposite shortly as Ag2S@C). The nanostructure exhibits strong fluorescent emission at λex/λem = 340/450 nm with a quantum yield of 12.57 ± 0.52%. Ag2S@C is bound to E. coli O157:H7 via strong interaction with the Mans moiety in Ag2S@C with FimH proteins on the fimbriae tip in E...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28722428/bsa-sugar-conjugates-as-ideal-building-blocks-for-spr-based-glycan-biosensors
#5
Shun Tao, Tian-Wei Jia, Yang Yang, Li-Qiang Chu
Controlled immobilization of sugar probes is of key importance for the development of glycan biosensors. To this end, a series of BSA-sugar conjugates with different numbers of mannose units are prepared via the squaric acid-mediated coupling reaction. The conjugates can absorb directly on gold substrate without any derivation reactions, thus providing a simple and effective method for the construction of SPR-based glycan biosensors. SPR measurements show that the BSA-mannose conjugate with 11 mannoses exhibit the highest affinity to the lectin concanavalin A with a limit of detection of ca...
January 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28721855/cell-surface-physiology-and-outer-cell-envelope-impermeability-for-hydrophobic-substances-in-burkholderia-multivorans
#6
Sallie A Ruskoski, Franklin R Champlin
PURPOSE: The purpose of the present study was to obtain a better understanding of the relationship between cell surface physiology and outer cellular envelope permeability for hydrophobic substances in mucoid and non-mucoid B. multivorans strains, as well as in two capsule-deficient derivatives of a mucoid parental strain. METHODOLOGY: Cell surface hydrophobicity properties were determined using the hydrocarbon adherence method, while outer cell envelope accessibility and permeability for non-polar compounds were measured using hydrophobic antimicrobial agent susceptibility and fluorescent probe assays...
July 18, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28719181/differential-ligand-binding-specificities-of-the-pulmonary-collectins-are-determined-by-the-conformational-freedom-of-a-surface-loop
#7
Michael J Rynkiewicz, Huixing Wu, Tanya R Cafarella, Nikolaos M Nikolaidis, James Frederick Head, Barbara A Seaton, Francis X McCormack
Lung surfactant proteins (SP) play critical roles in surfactant function and innate immunity. SP-A and SP-D, members of the collectin family of C-type lectins, exhibit distinct ligand specificities, effects on surfactant structure, and host defense functions despite extensive structural homology. SP-A binds to dipalmitoylphosphatidylcholine (DPPC), the major surfactant lipid component, but not phosphatidylinositol (PI), whereas SP-D shows the opposite preference. Additionally, SP-A and SP-D recognize widely divergent pathogen-associated molecular patterns...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28716948/cooperative-repression-of-igf2r-translation-by-mir-195-and-rna-binding-protein-cugbp1
#8
Yuan Zhang, Yun Zhang, Lan Xiao, Ting-Xi Yu, Jun-Zhe Li, Jaladanki N Rao, Douglas J Turner, Myriam Gorospe, Jian-Ying Wang
Insulin-like growth factor type-2 (IGF2) receptor (IGF2R) recognizes mannose 6-phosphate-containing molecules and IGF2 and plays an important role in many pathophysiological processes including gut mucosal adaptation. However, the mechanisms that control cellular IGF2R abundance are poorly known. MicroRNAs (miRNAs) and RNA-binding proteins (RBP) critically regulate gene expression programs in mammalian cells by modulating the stability and translation of target mRNAs. Here we report that miRNA-195 (miR-195) and the RBP CUG-binding protein 1 (CUGBP1) jointly regulate IGF2R expression at the posttranscriptional level in intestinal epithelial cells...
July 17, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28715863/structural-elucidation-of-a-pectin-type-polysaccharide-from-hovenia-dulcis-peduncles-and-its-proliferative-activity-on-raw264-7-cells
#9
Miaomiao Wang, Yong Liu, Mingliang Qiang, Junhui Wang
In this paper, an acidic polysaccharide HDP3A was isolated from Hovenia dulcis peduncles with 45.9% of uronic acid content. Structure of HDP3A was elucidated by chemical and spectroscopic analysis. HDP3A was mainly contained of galacturonic acid, galactose, rhamnose and arabinose with a small quantity of xylose, fucose, mannose, glucose. The structural analysis indicated that HDP3A was a pectin-type polysaccharide with HG and RG-I regions and hairy regions. HG was linear homopolymer with repeating units of (1→4)-α-d-galactopyranosyl uronic (GalA) residues...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28715682/cath-2-and-ll-37-increase-mannose-receptor-expression-antigen-presentation-and-the-endocytic-capacity-of-chicken-mononuclear-phagocytes
#10
Marina D Kraaij, Albert van Dijk, Henk P Haagsman
Cathelicidins display in vitro and in vivo immunomodulatory activities and are part of the innate immune system. Previously, we found that in ovo treatment with chicken cathelicidin CATH-2 partially protects young broilers against respiratory E. coli infection. To determine the cellular aspects of this protection, we investigated immunomodulatory effects of CATH-2 and the human cathelicidin LL-37 on primary chicken peripheral blood mononuclear cells (PBMCs). Treatment of chicken PBMCs with L-CATH-2, D-CATH-2 or LL-37 increased the percentage of mononuclear phagocytes, but decreased that of B cells...
July 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28715515/surgical-suture-releasing-macrophage-targeted-drug-loaded-nanoparticles-for-an-enhanced-anti-inflammatory-effect
#11
Hansol Kim, Byung Hwi Kim, Beom Kang Huh, Yeon Chun Yoo, Chan Yeong Heo, Young Bin Choy, Ji-Ho Park
A surgical suture is a medical device to close the wound site of skin and organs but excessive inflammation surrounding the suture can disrupt the wound healing process. Although post-operative prescription of anti-inflammatory drugs is used to manage the inflammation, the need for local drug delivery systems has been rising because of low bioavailability and fast clearance of drugs. In this work, we proposed a new strategy for a local anti-inflammatory device by incorporating macrophage-targeted anti-inflammatory nanoparticles into the suture...
July 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28714032/atf3-promotes-migration-and-m1-m2-polarization-of-macrophages-by-activating-tenascin%C3%A2-c-via-wnt-%C3%AE-%C3%A2-catenin-pathway
#12
Hao Sha, Dianzhong Zhang, Yunfei Zhang, Yanhua Wen, Yucai Wang
There are different polarization states of macrophages, including the classically activated M1 phenotype and the alternatively activated M2 phenotype. These have different functions in the inflammation process. Activating transcription factor 3 (ATF3) is a key transcriptional regulator that inhibits the inflammatory response. However, the effects of ATF3 on migration and anti‑inflammatory control mechanisms of macrophages have not been thoroughly investigated. The present study investigated the effect of ATF3 on macrophage migration and M1/M2 polarization...
July 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28714029/involvement-of-gut-microbiota-in-the-association-between-gastrointestinal-motility-and-5%C3%A2-ht-expression-m2-macrophage-abundance-in-the-gastrointestinal-tract
#13
Mo Yang, Hirokazu Fukui, Hirotsugu Eda, Yoshitaka Kitayama, Ken Hara, Mio Kodani, Toshihiko Tomita, Tadayuki Oshima, Jiro Watari, Hiroto Miwa
Serotonin (5‑hydroxytryptamine; 5‑HT) may be a key player in gastrointestinal (GI) motility and the GI immune system. In the present study, the effect of gut microbiota on the association between GI motility, and 5‑HT expression and macrophage abundance in the GI tract was examined. Germ‑free (GF) mice (6 weeks old) were orally administered a fecal bacterial suspension prepared from specific pathogen‑free mice and their GI tissues were evaluated 4 weeks later. The expression of 5‑HT and mannose receptor (MR) was examined by immunohistochemistry, and GI transit time (GITT) was measured by administration of carmine red solution...
July 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28711789/towards-a-better-understanding-of-protein-changes-in-common-bean-under-drought-a-case-study-of-n-glycoproteins
#14
Tanja Zadražnik, Anders Moen, Wolfgang Egge-Jacobsen, Vladimir Meglič, Jelka Šuštar-Vozlič
Drought is one of the major abiotic stress conditions limiting crop growth and productivity. Glycosylation of proteins is very important post-translational modification that is involved in many physiological functions and biological pathways. To understand the involvement of N-glycoproteins in the mechanism of drought response in leaves of common bean, a proteomic approach using lectin affinity chromatography, SDS-PAGE and LC-MS/MS was applied. Quantification of N-glycoproteins was performed using MaxQuant with a label free quantification approach...
July 5, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28711614/antioxidant-and-antibacterial-activities-of-polysaccharides-isolated-and-purified-from-diaphragma-juglandis-fructus
#15
Qingran Meng, Yinghao Li, Tiancun Xiao, Lianfu Zhang, Dan Xu
A water-soluble polysaccharide fraction (DJP-2) isolated from Diaphragma juglandis was successfully purified by ion-exchange chromatography (DEAE-cellulose) and gel-permeation chromatography (Sephadex G-100). The weight-average molecular weight (Mw) and number-average molecular weight (Mn) of DJP-2 were 4.95 and 3.99kDa, respectively. Monosaccharide component analysis indicated that DJP-2 comprised arabinose, galactose, glucose, xylose, and mannose in a molar ratio of 0.27:0.55:1:0.14:0.08. The evaluation of the antioxidant and antibacterial activities of polysaccharides from Diaphragma juglandis fructus indicated that they could be explored as promising natural antioxidant and bacteriostatic agents in the food and pharmaceutical industries...
July 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28709621/role-of-n-acetyl-galactosamine-4-so4-a-ligand-of-cd206-in-hsv-induced-mouse-model-of-beh%C3%A3-et-s-disease
#16
Bunsoon Choi, Hasan M Sayeed, S M Shamsul Islam, Seonghyang Sohn
CD206 is a macrophage mannose receptor involved in variety of autoimmune and inflammatory diseases. This study aimed to identify the pathogenic role of CD206 in a herpes simplex virus (HSV) induced Behçet's disease (BD) mouse model. CD206 positive cells were detected in peripheral blood mononuclear cells and quantified by flow cytometry. Levels of cytokines were measured by ELISA. CD206 was found to be down-regulated both in vitro (10(-6) M) and in vivo (200μg/mouse) after treatment with N-acetylgalactosamine (GalNAc), a ligand for CD206...
July 11, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28709130/bacterial-adhesins-the-pathogenic-weapons-to-trick-host-defense-arsenal
#17
REVIEW
Seema Patel, Nithya Mathivanan, Arun Goyal
Adhesins are bacterial proteins with host cell adhesive properties. These proteins occur in diverse architectures, ranging from capsules, vesicles, pili, fimbri, to enzymes. These proteins interact with host cell surface receptor proteins, for cross-membrane- trafficking and the invasion of host cells. Thus, they lead to inflammation and pathogenesis, of chronic as well as acute type. Inhibition of adhesin-mediated immune activation can be possible by mannose supplementation, assembly disruption, and host receptor blockage, among other approaches...
July 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28706280/radiation-induced-changes-in-the-glycome-of-endothelial-cells-with-functional-consequences
#18
Cyprien Jaillet, Willy Morelle, Marie-Christine Slomianny, Vincent Paget, Georges Tarlet, Valérie Buard, Sonia Selbonne, Fanny Caffin, Emilie Rannou, Pierre Martinez, Agnès François, François Foulquier, Fabrice Allain, Fabien Milliat, Olivier Guipaud
As it is altered by ionizing radiation, the vascular network is considered as a prime target in limiting normal tissue damage and improving tumor control in radiation therapy. Irradiation activates endothelial cells which then participate in the recruitment of circulating cells, especially by overexpressing cell adhesion molecules, but also by other as yet unknown mechanisms. Since protein glycosylation is an important determinant of cell adhesion, we hypothesized that radiation could alter the glycosylation pattern of endothelial cells and thereby impact adhesion of circulating cells...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28706259/genetic-polymorphisms-of-c-type-lectin-receptors-in-behcet-s-disease-in-a-chinese-han-population
#19
Yi Yang, Handan Tan, Bolin Deng, Hongsong Yu, Guannan Su, Jiayue Hu, Qingfeng Cao, Gangxiang Yuan, Aize Kijlstra, Peizeng Yang
C-type lectin receptors (CLRs) have been demonstrated to be involved in several autoimmune diseases. The role of CLRs in Behcet's disease (BD) is unknown and thus was the purpose of this study. A two-stage association study was carried out and a total of 766 BD patients and 1674 healthy controls were recruited. Genotyping of 14 SNPs of 13 genes in CLRs was carried out by iPLEX Gold genotyping or polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The expression of mannose binding lectin 2 (MBL2) and killer cell lectin like receptor C4 (KLRC4) was measured by Real-time PCR...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28705043/mannosylated-liposomes-improve-therapeutic-effects-of-paclitaxel-in-colon-cancer-models
#20
Menghua Xiong, Qian Lei, Xinyu You, Tiantao Gao, Xuejiao Song, Yong Xia, Tinghong Ye, Lidan Zhang, Ningyu Wang, Luoting Yu
Mannose receptor (MR) is a highly effective endocytic receptor. It is closely related to tumor immune escape and metastasis. We found that MR was highly expressed in some colon cancer cell lines such as CT26 and HCT116 cells. Therefore MR might be a potential target in colon cancer therapy. In this study, we aimed to develop mannosylated liposomes containing anticancer drug paclitaxel (PTX) and investigate the potential effects on targeted therapy for colon cancer. Mannosylated liposomes were prepared by film dispersion method...
July 14, 2017: Journal of Microencapsulation
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