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https://www.readbyqxmd.com/read/28524185/erythrocytes-based-quartz-crystal-microbalance-cytosensor-for-in-situ-detection-of-cell-surface-sialic-acid
#1
Xiaojuan Yang, Lin Zhou, Yan Hao, Bin Zhou, Peihui Yang
An erythrocytes (RBCs) cytosensor was first fabricated for in situ analysis of sialic acid (SA) on the cell surface based on a quartz crystal microbalance (QCM). RBCs, as a recognition element, were immobilized on a concanavalin A (ConA)-modified gold chip through the specific recognition between ConA and mannose on the cell surface. 4-Aminobenzeneboronic acid (APBA)-functionalized gold nanoparticles (AuNPs/APBA) were used as a signal amplification nanoprobe for labeling SA on the surface of RBCs. Compared to that of APBA, the frequency response of the cytosensor could be significantly enhanced 18-fold by using a AuNPs/APBA nanoprobe...
May 19, 2017: Analyst
https://www.readbyqxmd.com/read/28524080/cereus-sinensis-polysaccharide-and-its-immunomodulatory-properties-in-human-monocytic-cells
#2
Junwen Wu, Xuefei Zhou, Min Zhang, Yun Yao, Juanjuan Han, Kehai Liu
In this study, the extraction conditions of the crude polysaccharide from Cereus sinensis were optimized by response surface methodology. The optimum extraction conditions were: a ratio of raw material to water volume of 1:80 (g/mL); an extraction temperature of 72 °C; and an extraction time of 3 h. Then, a purified polysaccharide named Cereus sinensis polysaccharide-1 (CSP-1) was obtained from the crude polysaccharide by the Diethylaminoethyl cellulose-52 (DEAE-52) cellulose chromatography column and Sephadex G-100 column...
May 18, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28521980/sulphated-polysaccharide-isolated-from-sargassum-vulgare-characterization-and-hypolipidemic-effects
#3
Rihab Ben Abdallah Kolsi, Hichem Ben Salah, Neila Jardak, Rim Chaaben, Imed Jribi, Abdelfattah El Feki, Tarak Rebai, Kamel Jamoussi, Noureddine Allouche, Cristophe Blecker, Hafedh Belghith, Karima Belghith
A sulphated polysaccharide from brown algae Sargassum vulgare (SVSP) was extracted and examined with respect to chemical, structural characterization and hypolipidemic effects. SVSP consisted mainly of sulphate and total sugars with low levels of lipids and proteins. Its structure was studied by nuclear magnetic resonance (RMN), gas chromatography-mass spectrometry (GC-MS), infra-red spectroscopic, differential scanning calorimetry and X-ray diffraction analysis. Allowing us therefore to revealed that SVSP was composed of glucose, rhamnose, xylose, galactose, mannose and arabinose with XRD pattern that was typical for a semi-crystalline polymer and complexities of the spectra reflected its homogeneous structure...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28512129/mammalian-o-mannosylation-of-cadherins-and-plexins-is-independent-of-protein-o-mannosyltransferase-1-and-2
#4
Ida Signe Bohse Larsen, Yoshiki Narimatsu, Hiren Jitendra Joshi, Zhang Yang, Oliver J Harrison, Julia Brasch, Lawrence Shapiro, Barry Honig, Sergey Y Vakhrushev, Henrik Clausen, Adnan Halim
Protein O-mannosylation is found in yeast and metazoans and a family of conserved orthologous protein O-mannosyltransferases is believed to initiate this important post-translational modification. We recently discovered that the cadherin superfamily carries O-linked mannose (O-Man) glycans at highly conserved residues in specific extracellular cadherin domains, and it was suggested that the function of E-cadherin was dependent on the O-Man glycans. Deficiencies in enzymes catalyzing O-Man biosynthesis, including the two human protein O-mannosyltransferases, POMT1 and POMT2, underlie a subgroup of congenital muscular dystrophies (CMD) designated α-dystroglycanopathies, because deficient O-Man glycosylation of -dystroglycan disrupts laminin interaction with -dystroglycan and the extracellular matrix...
May 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28510654/asn-linked-oligosaccharide-chain-of-a-crenarchaeon-pyrobaculum-calidifontis-is-reminiscent-of-the-eukaryotic-high-mannose-type-glycan
#5
Daisuke Fujinami, Yuya Taguchi, Daisuke Kohda
Pyrobaculum calidifontis is a hyperthermophilic archaeon that belongs to the phylum Crenarchaeota. In contrast to the phylum Euryarchaeota, only the N-glycan structure of the genus Sulfolobus is known in Crenarchaeota. Here, we enriched glycoproteins from cultured P. calidifontis cells, by ConA lectin chromatography. The MASCOT search identified proteins with at least one potential N-glycosylation site. The MS/MS analysis of twelve small tryptic glycopeptides confirmed the canonical N-glycosylation consensus in P...
May 16, 2017: Glycobiology
https://www.readbyqxmd.com/read/28509971/description-of-chryseobacterium-timonianum-sp-nov-isolated-from-a-patient-with-pneumonia
#6
Rita Abou Abdallah, Teresa Cimmino, Sophie Baron, Frédéric Cadoret, Caroline Michelle, Didier Raoult, Pierre-Edouard Fournier, Fadi Bittar
Using a polyphasic taxonomic strategy, an aerobic, Gram-negative, non-motile, yellow pigmented rod isolated from a sputum sample of a patient with pneumonia was characterised. This bacterial strain, designated G972(T), could not be identified by our systematic MALDI-TOF screening on a MicroFlex. This led to the sequencing of the 16S rRNA gene, which shows 98.57% sequence identity with that of Chryseobacterium indologenes 16777(T), the phylogenetic closely related type strain of a species with standing in nomenclature, which putatively classifies it as a new species...
May 16, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28509550/sugars-require-rigid-multivalent-displays-for-activation-of-mouse-sperm-acrosomal-exocytosis
#7
He Huang, Maria T Rodolis, Surita Rani Bhatia, Nicole S Sampson
As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose or GlcNAc are effective synthetic inducers of mouse sperm acrosomal exocytosis (AE). Each carbohydrate is proposed to have a distinct binding site on the sperm cell surface. To determine the role of the scaffold structure on efficiency of AE induction, different polymer backbones were employed to display the different activating sugar residues...
May 16, 2017: Biochemistry
https://www.readbyqxmd.com/read/28509534/analysis-of-three-epoetin-alpha-products-by-lc-and-lc-ms-indicates-differences-in-glycosylation-critical-quality-attributes-including-sialic-acid-content
#8
Rebecca I Thomson, Richard Andrew Gardner, Katja Strohfeldt, Daryl L Fernandes, Graham Philip Stafford, Daniel I R Spencer, Helen M I Osborn
Erythropoietin (EPO) is one of the main treatments for anaemic patients, greatly improving their quality of life. In this study, biosimilars Binocrit and a development product, called here CIGB-EPO, were compared to the originator product, Eprex. All three are epoetin alpha products, reputed to have similar glycosylation profiles. The quality, safety and efficacy of this biotherapeutic depend on the following glycosylation critical quality attributes (GCQAs): sialylation, N-glycolyl-neuraminic acid (Neu5Gc) content, branching, N-acetyl-lactosamine (LacNAc) extensions and, arguably, O-acetylation pattern...
May 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28509333/altered-glycan-accessibility-on-native-immunoglobulin-g-complexes-in-early-rheumatoid-arthritis-and-its-changes-during-therapy
#9
Judith Stümer, Mona H C Biermann, Jasmin Knopf, Iryna Magorivska, Alf Kastbom, Anna Svärd, Christina Janko, Rostyslav Bilyy, Georg Schett, Christopher Sjöwall, Martin Herrmann, Luis E Muñoz
The goal of this study was to investigate the glycosylation profile of native IgG present in serum immune complexes in patients with rheumatoid arthritis (RA). To accomplish this, lectin binding assays, detecting the accessibility of glycans present on IgG-containing immune complexes by biotinylated lectins were employed. Lectins capturing fucosyl residues (AAL), fucosylated tri-mannose N-glycan core sites (LCA), terminal sialic acid residues (SNA) and O-glycosidically-linked galactose/N-acetylgalactosamine (GalNac-L) were used...
May 16, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28507200/effects-of-microvirin-mono-and-oligomers-on-hepatitis-c-virus
#10
Yuan Qin Min, Xu Chu Duan, Yi Dan Zhou, Anna Kulinich, Meng Wang, Zhi-Peng Cai, Hong Yu Ma, Li Liu, Xiao Lian Zhang, Josef Voglmeir
Microvirin is a carbohydrate binding protein which shows high specificity for high-mannose type N-glycan structures. In this study we tried to identify whether microvirin could bind to high-mannose containing Hepatitis C Virus (HCV) envelope glycoproteins, which are heavily decorated decorated high-mannose glycans. In addition, recombinantly expressed microvirin oligomers in di-, tri-, and tetrameric form were evaluated for their viral inhibition. Microvirin oligomers bound more efficiently to HCV virions, and displayed in comparison to the microvirin monomer a higher neutralisation potency against HCV infection...
May 15, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28506987/development-of-the-larval-lymphatic-system-in-the-zebrafish
#11
Hyun Min Jung, Daniel Castranova, Matthew R Swift, Van N Pham, Marina Venero Galanternik, Sumio Isogai, Matthew G Butler, Timothy S Mulligan, Brant M Weinstein
The lymphatic vascular system is a hierarchically organized complex network essential for tissue fluid homeostasis, immune trafficking, and absorption of dietary fats in the human body. Despite its importance, the assembly of the lymphatic network is still not fully understood. The zebrafish is a powerful model organism that enables study of lymphatic vessel development using high-resolution imaging and sophisticated genetic and experimental manipulation. Although several studies have described early lymphatic development in the fish, lymphatic development at later stages has not been completely elucidated...
May 15, 2017: Development
https://www.readbyqxmd.com/read/28506090/rational-design-strategies-for-fimh-antagonists-new-drugs-on-the-horizon-for-urinary-tract-infection-and-crohn-s-disease
#12
Laurel K Mydock-McGrane, Thomas J Hannan, James W Janetka
The bacterial adhesin FimH is a virulence factor and an attractive therapeutic target for urinary tract infection (UTI) and Crohn's Disease (CD). Located on type 1 pili of uropathogenic E. coli (UPEC), the FimH adhesin plays an integral role in the pathogenesis of UPEC. Recent efforts have culminated in the development of small-molecule mannoside FimH antagonists that target the mannose-binding lectin domain of FimH, inhibiting its function and preventing UPEC from binding mannosylated host cells in the bladder, thereby circumventing infection...
May 16, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28504124/structural-characterization-and-rheological-properties-of-a-gel-like-%C3%AE-d-glucan-from-pholiota-nameko
#13
REVIEW
Vanessa Sovrani, Liana Inara de Jesus, Fernanda Fogagnoli Simas-Tosin, Fhernanda Ribeiro Smiderle, Marcello Iacomini
A crude fraction (SCW) was extracted with cold water from Pholiota nameko and showed mannose (24.1%), galactose (44.9%) and glucose (31%). Purification procedures resulted in a polysaccharide fraction (bG-PN), that showed only glucose. NMR and methylation analyses of bG-PN indicated a β-d-glucan-(1→3)-linked, substituted at O-6 by β-d-Glcp or (1→6)-linked β-d-Glcp side chains. Rheological studies of crude and purified fractions at the same concentration showed similar shear-thinning behavior and gel-like structure which indicates no need to isolate the polymer to achieve some desirable rheological properties...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28499970/chitinase1-contributed-to-a-potential-protection-via-microglia-polarization-and-a%C3%AE-oligomer-reduction-in-d-galactose-and-aluminum-induced-rat-model-with-cognitive-impairments
#14
Qian Xiao, Weihua Yu, Qi Tian, Xue Fu, Xia Wang, Min Gu, Yang Lü
Chitinase activity is increased in Alzheimer's disease (AD). However, the role of chitinase1 in AD is unknown. We investigated the effects of chitinase1 on Alzheimer's pathology and microglia function. Artificial chitinase1 and chitinase inhibitor (chitinase-IN-2) were used to determine the effects of chitinase1 on inflammatory factors and β-amyloid (Aβ) oligomer deposition in D-galactose/AlCl3-induced rat model with cognitive impairments. Aβ-treated N9 microglia cells were analyzed to further verify whether the changes in inflammatory factors following chitinase1 treatment were associated with microglia alternative activation...
May 10, 2017: Neuroscience
https://www.readbyqxmd.com/read/28498622/regulation-of-raw264-7-macrophage-polarization-on-smooth-and-rough-surface-topographies-by-galectin-3
#15
F Kianoush, M Nematollahi, J D Waterfield, D M Brunette
Recognition of topographical features induces phenotypic changes in macrophages although the receptors and signaling pathways are not completely characterized. As integrin molecules in focal adhesions/podosomes are in intimate contact with topography and topography modulates the NFkB pathway through cholesterol enriched raft-associated adhesive signaling structures we hypothesized that a cell-surface signaling complex comprised of galectin-3 together with its ligand CD98 and integrinβ1 is important for topography-directed lineage determination...
May 12, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28498105/intracellular-uptake-of-macromolecules-by-brain-lymphatic-endothelial-cells-during-zebrafish-embryonic-development
#16
Max van Lessen, Shannon Shibata-Germanos, Andreas van Impel, Thomas A Hawkins, Jason Rihel, Stefan Schulte-Merker
The lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and found loosely connected endothelial cells with lymphatic molecular signature covering parts of the brain without forming endothelial tubular structures. These brain lymphatic endothelial cells (BLECs) derive from venous endothelium, are distinct from macrophages, and are sensitive to loss of Vegfc...
May 12, 2017: ELife
https://www.readbyqxmd.com/read/28496990/mtorc1-regulates-mannose-6-phosphate-receptor-transport-and-t-cell-vulnerability-to-regulatory-t-cells-by-controlling-kinesin-kif13a
#17
Khawaja Ashfaque Ahmed, Jim Xiang
Mannose-6-phosphate receptor (M6PR) that facilitates cellular uptake of M6P-bearing proteins, including serine-protease granzyme-B (Gzm-B) has an important role in T-cell activation, migration and contraction. However, molecular mechanisms controlling M6PR expression in T cells remain poorly understood. Here, we show that M6PR expression on T cells is distinctively controlled by two common γ-chain cytokines interleukin-2 (IL-2) and IL-7, and the differential M6PR expression is not caused by an altered synthesis of M6PR protein, but is a result of distinct regulation of kinesin-3 motor-protein KIF13A that transport M6PR onto cell surfaces...
2017: Cell Discovery
https://www.readbyqxmd.com/read/28496488/purification-characterization-and-antioxidant-activities-of-enzymolysis-polysaccharide-from-grifola-frondosa
#18
Zhao Ting, Fan Yina, Mao Guanghua, Feng Weiwei, Zou Yanmin, Zou Ye, Yang Liuqing, Wu Xiangyang
Our previous study revealed that the antioxidant activity of polysaccharide (coded as FGFP) extracted from Grifola frondosa by enzymolysis treatment was significantly superior than that (coded as GFP) extracted by boiling-water. In this study, one purified polysaccharide fractions (coded as FGFP-11) was obtained from FGFP by purified using DEAE-52 column and Sephacryl S-500HR column. Results indicated that FGFP-11 with MW of 59.82 kDa consisted of mannose, glucose and galactose with a molar ratio of 1.00:16...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28495090/separation-of-ten-phosphorylated-mono-and-disaccharides-using-hilic-and-ion-pairing-interactions
#19
Caroline Mathon, Gregory A Barding, Cynthia K Larive
Phosphorylated carbohydrates are indispensable cogs in several key metabolic wheels for all forms of life. Here, a straightforward liquid chromatography method coupled to mass spectrometry detection was developed for phosphorylated sugars. For separation of the targeted compounds, hydrophilic interaction chromatography (HILIC) was used with a bridged-ethylene hybrid amide column under alkaline conditions using triethylamine as a mobile phase modifier. Methylphosphonic acid was added to the aqueous mobile phase to reduce the tailing of compounds containing phosphate groups, which are known to interact with stainless steel components of the separation system...
June 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28494932/comparison-of-growth-performance-and-immune-parameters-of-three-commercial-chicken-lines-used-in-organic-production
#20
R B Kjærup, H R Juul-Madsen, L R Norup, P Sørensen, T S Dalgaard
Owing to the higher demands for avoiding medication and antibiotics, health status of the production animals plays an important role in the poultry industry, especially in organic poultry systems. Immunity plays a major role in keeping the host free from disease, and it is evident that the host's genetic make-up influences immunity and disease resistance/susceptibility in chickens. Previously, breeding strategies aimed at selection for resistance against specific diseases with the risk of creating less disease resistance against other pathogens...
May 2017: Veterinary Immunology and Immunopathology
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