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https://www.readbyqxmd.com/read/28235607/the-preparation-of-three-selenium-containing-cordyceps-militaris-polysaccharides-characterization-and-anti-tumor-activities
#1
Fei Liu, Zhen-Yuan Zhu, Xiaoli Sun, Hui Gao, Yong-Min Zhang
In the present work, three fractions of selenized Cordyceps militaris polysaccharides (SeCPS) named SeCPS-I, SeCPS-II and SeCPS-III were isolated and purified by ultra-filtration. Their selenium content were measured as 541.3, 863.7 and 623.3μg/g respectively by a graphite furnace atomic absorption spectroscopy. The monosaccharide comformation analysis showed that they were mainly consisted of D-Mannose, D-Glucose, and D-Galactose in mole ratios of 1:7.63:0.83, 1:1.34:0.31 and 1:3.77:0.41 respectively. Their structure characteristics were compared by IFR and NMR spectroscopy...
February 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28223515/rational-design-of-adjuvants-targeting-the-c-type-lectin-mincle
#2
Alexiane Decout, Sandro Silva-Gomes, Daniel Drocourt, Sophie Barbe, Isabelle André, Francisco J Cueto, Thierry Lioux, David Sancho, Eric Pérouzel, Alain Vercellone, Jacques Prandi, Martine Gilleron, Gérard Tiraby, Jérôme Nigou
The advances in subunit vaccines development have intensified the search for potent adjuvants, particularly adjuvants inducing cell-mediated immune responses. Identification of the C-type lectin Mincle as one of the receptors underlying the remarkable immunogenicity of the mycobacterial cell wall, via recognition of trehalose-6,6'-dimycolate (TDM), has opened avenues for the rational design of such molecules. Using a combination of chemical synthesis, biological evaluation, molecular dynamics simulations, and protein mutagenesis, we gained insight into the molecular bases of glycolipid recognition by Mincle...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28222660/potential-therapeutic-application-of-dendrimer-cyclodextrin-conjugates-with-targeting-ligands-as-advanced-carriers-for-gene-and-oligonucleotide-drugs
#3
Hidetoshi Arima, Keiichi Motoyama, Taishi Higashi
Despite the recent approval of some gene medicines and nucleic acid drugs, further improvement of delivery techniques for these drugs is strongly required. Several delivery technologies for these drugs have been developed, in other words, viral and two types of nonviral (lipofection and polyfection) vectors. Among the polyfection system, the potential use of various cyclodextrin (CyD) derivatives and CyD-appended polymers as carriers for gene and nucleic acid drugs has been demonstrated. The polyamidoamine dendrimer (G3) conjugates with α-CyD (α-CDE (G3)) have been reported to possess noteworthy properties as DNA and nucleic acid drugs carriers...
April 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/28221015/taking-orders-from-light-photo-switchable-working-inactive-smart-surfaces-for-protein-and-cell-adhesion
#4
Junji Zhang, Wenjing Ma, Xiao-Peng He, He Tian
Photo-responsive smart surfaces are promising candidates for a variety of applications in optoelectronics and sensing devices. The use of light as an order-signal provides advantages of remote and non-invasive control with high temporal and spatial resolutions. Modification of the photo-switches with target bio-macromolecules, such as peptides, DNA and small-molecules including folic acid derivatives and sugars, has recently become a popular strategy to empower the smart surfaces with an improved detection efficiency and specificity...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28220352/purification-characterization-and-overexpression-of-an-endo-1-4-%C3%AE-mannanase-from-thermotolerant-bacillus-sp-swu60
#5
Weeranuch Seesom, Polphet Thongket, Tomohiro Yamamoto, Shigeo Takenaka, Tatsuji Sakamoto, Wasana Sukhumsirichart
Endo-β-1,4-mannanases are important catalytic agents in several industries. The enzymes randomly cleave the β-1,4-linkage in the mannan backbone and release short β-1,4-mannooligosaccharides and mannose. In the present study, mannanase (ManS2) from thermotolerant Bacillus sp. SWU60 was purified, characterized, and its gene was cloned and overexpressed in Escherichia coli. ManS2 was purified from culture filtrate (300 ml) by using hydrophobic, ion-exchange, and size-exclusive liquid chromatography. The apparent molecular mass was 38 kDa...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28219886/dependence-of-glomerulonephritis-induction-on-novel-intraglomerular-alternatively-activated-bone-marrow-derived-macrophages-and-mac-1-and-pd-l1-in-lupus-prone-nzm2328-mice
#6
Sun-Sang J Sung, Yan Ge, Chao Dai, Hongyang Wang, Shu Man Fu, Rahul Sharma, Young S Hahn, Jing Yu, Thu H Le, Mark D Okusa, Warren K Bolton, Jessica R Lawler
Glomerular damage mediated by glomerulus-infiltrating myeloid-derived cells is a key pathogenic event in lupus nephritis (LN), but the process is poorly understood. Confocal microscopy of kidney sections and flow cytometry analysis of glomerular cells from magnetic bead-purified glomeruli have identified glomerulus-infiltrating leukocyte populations in NZM2328 (NZM) lupus-prone mice with spontaneous chronic glomerulonephritis (GN) and anti-glomerular basement membrane-induced nephritis. The occurrence of a major glomerulus-infiltrating CD11b(+)F4/80(-)I-A(-) macrophage population exhibiting the markers programmed death ligand-1 (PD-L1), Mac-2, and macrophage mannose receptor (CD206) and producing Klf4, Il10, Retnla, Tnf, and Il6 mRNA, which are known to be expressed by alternatively activated (M2b) macrophages, correlated with proteinuria status...
February 20, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28213261/a-method-for-the-analysis-of-sugars-in-biological-systems-using-reductive-amination-in-combination-with-hydrophilic-interaction-chromatography-and-high-resolution-mass-spectrometry
#7
Sami Bawazeer, Ali Muhsen Ali, Aliyah Alhawiti, Abedawn Khalaf, Colin Gibson, Jonans Tusiimire, David G Watson
Separation of sugar isomers extracted from biological samples is challenging because of their natural occurrence as alpha and beta anomers and, in the case of hexoses, in their pyranose and furanose forms. A reductive amination method was developed for the tagging of sugars with the aim of it becoming part of a metabolomics work flow. The best separation of the common hexoses (glucose, fructose, mannose and galactose) was achieved when (2)H5-aniline was used as the tagging reagent in combination with separation on a ZICHILIC column...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28207759/investigations-on-therapeutic-glucocerebrosidases-through-paired-detection-with-fluorescent-activity-based-probes
#8
Wouter W Kallemeijn, Saskia Scheij, Sascha Hoogendoorn, Martin D Witte, Daniela Herrera Moro Chao, Cindy P A A van Roomen, Roelof Ottenhoff, Herman S Overkleeft, Rolf G Boot, Johannes M F G Aerts
Deficiency of glucocerebrosidase (GBA) causes Gaucher disease (GD). In the common non-neuronopathic GD type I variant, glucosylceramide accumulates primarily in the lysosomes of visceral macrophages. Supplementing storage cells with lacking enzyme is accomplished via chronic intravenous administration of recombinant GBA containing mannose-terminated N-linked glycans, mediating the selective uptake by macrophages expressing mannose-binding lectin(s). Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans...
2017: PloS One
https://www.readbyqxmd.com/read/28206680/an-ssdna-aptamer-against-mannose-capped-lipoarabinomannan-enhances-anti-tuberculosis-activity-of-macrophages-through-downregulation-of-lipid-sensing-nuclear-receptor-peroxisome-proliferator-activated-receptor-%C3%AE-expression
#9
Qin Pan, Jiamin Yan, Qi Liu, Chunhui Yuan, Xiao-Lian Zhang
Mannose-capped lipoarabinomannan (ManLAM) is an immunomodulatory epitope of Mycobacterium tuberculosis (Mtb). We previously generated an aptamer (ZXL1) that specifically binds to ManLAM from the virulent Mtb H37Rv strain and reported that ZXL1 functioned as an antagonist, inhibiting the ManLAM-induced immunosuppression of dendritic cells (DCs). In the present study, we found that ZXL1 inhibited Mtb entry into murine macrophages. ZXL1 enhanced IL-1β and IL-12 mRNA expression and cytokine production in ManLAM-treated macrophages but decreased IL-10 production...
February 16, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28203237/activation-of-the-wnt-pathway-by-mycobacterium-tuberculosis-a-wnt-wnt-situation
#10
REVIEW
Tomás Villaseñor, Edgardo Madrid-Paulino, Rafael Maldonado-Bravo, Antonio Urbán-Aragón, Leonor Pérez-Martínez, Gustavo Pedraza-Alva
Mycobacterium tuberculosis (M. tuberculosis), an intracellular pathogenic Gram-positive bacterium, is the cause of tuberculosis (TB), a major worldwide human infectious disease. The innate immune system is the first host defense against M. tuberculosis. The recognition of this pathogen is mediated by several classes of pattern recognition receptors expressed on the host innate immune cells, including Toll-like receptors, Nod-like receptors, and C-type lectin receptors like Dectin-1, the Mannose receptor, and DC-SIGN...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28199092/application-of-a-quantitative-lc-ms-multi-attribute-method-for-monitoring-site-specific-glycan-heterogeneity-on-a-monoclonal-antibody-containing-two-n-linked-glycosylation-sites
#11
Tian Wang, Lily Chu, Wenzhou Li, Kenneth A Lawson, Izydor Apostol, Tamer Eris
A significant challenge of traditional glycan mapping techniques is that they do not provide site-specific glycosylation information. Therefore, for proteins containing multiple glycosylation sites, the individual glycan species present at a particular site cannot be differentiated from those species present at the other glycosylation sites on the molecule. Quantification of glyco-form has previously been demonstrated using a multi-attribute method (MAM), which can quantify multiple post-translational modifications including deamidation, oxidation, glycosylation variants, and fragmentation1...
February 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28197138/genetic-and-phenotypic-screening-of-mannose-binding-lectin-in-relation-to-risk-of-recurrent-vulvovaginal-infections-in-women-of-north-india-a-prospective-cohort-study
#12
Namarta Kalia, Jatinder Singh, Sujata Sharma, Hardesh Arora, Manpreet Kaur
Recurrent Vulvovaginal Infections (RVVI) is common problem associated with women of reproductive age. The function and deleterious effect of Mannose Binding Lectin 2 (MBL2) common polymorphisms are reported to be associated with various diseases. However, the role of MBL2 promoter gene polymorphisms and their combined effect with structural variant along with Serum Mannose Binding Lectin (sMBL) levels in RVVI has not been investigated. The study included 258 RVVI cases and 203 age matched healthy controls. These were investigated for the distribution of MBL2 codon 54 and promoter polymorphisms by Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28192904/high-resolution-glycan-analysis-by-temperature-gradient-capillary-electrophoresis
#13
Marton Szigeti, Andras Guttman
Temperature gradient capillary electrophoresis was introduced to enhance separation selectivities for branched glycans of biotherapeutic interest. A mixture of afucosylated, fucosylated, and high mannose oligosaccharides was separated in the range of 15 to 45 °C at 5 °C temperature intervals. It was found that within this temperature range, the separation selectivity was carbohydrate structure dependent. The resolution between some glycan structures was greater at elevated temperatures, while others separated better at lower temperatures...
February 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192664/nmr-and-molecular-recognition-of-n-glycans-remote-modifications-of-the-saccharide-chain-modulate-binding-features
#14
Ana Gimeno, Niels-Christian Reichardt, Francisco Javier Cañada, Lukas Perkams, Carlo Unverzagt, Jesus Jimenez-Barbero, Ana Arda
Glycans play a key role as recognition elements in the communication of cells and other organisms. Thus, the analysis of carbohydrate-protein interactions has gained significant importance. In particular, Nuclear Magnetic Resonance (NMR) techniques are considered powerful tools to detect relevant features in the interaction between sugars and their natural receptors. Here we present the results obtained in the study on the molecular recognition of different mannose-containing glycans by Pisum Sativum Agglutinin...
February 13, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28188799/structure-elucidation-and-immunological-activity-of-a-novel-glycopeptide-from-mannatide
#15
Yanhong Shen, Mengqi Wu, Zhizhen Xu, Yanfei Wan, Li Zhang, Wenqing Zhang, Wei Xia
A water-soluble glycopeptide, designated as MTW, was isolated and purified from crude mannatide by DEAE-Sepharose. MTW showed one symmetrical peak on HPGPC with an average molecular weight of 2.95×10(4)Da. MTW contained 16 kinds of amino acids and the total amino acid content was 0.95%. The structure of polysaccharide moiety of MTW was elucidated based on monosaccharide composition, methylation analysis, partial acid hydrolysis, IR and 1D/2D NMR spectroscopy. The results showed that MTW was a homogeneous glycopeptide including mannose and glucose with a molar ratio of 2:1...
February 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28187228/effect-of-alprazolam-on-rat-serum-metabolic-profiles
#16
Yan Li, Gaotong Lin, Bingbao Chen, Jing Zhang, Lingtian Wang, Zixia Li, Yungang Cao, Congcong Wen, Xuezhi Yang, Gaozhong Cao, Xianqin Wang, Guoquan Cao
We developed a serum metabolomic method by gas chromatography-mass spectrometry (GC-MS) to evaluate the effect of alprazolam in rats. The GC-MS with HP-5MS (0.25 µm × 30 m × 0.25 mm), mass was conducted in EI mode with electron energy of 70 eV, full-scan mode with m/z 50-550. The rats were randomly divided to four group, three alprazolam treated group and control group. The alprazolam treated group rats were given 5, 10, 20 mg/kg (Low, Medium, High) of alprazolam by intragastric administration each day for 14 days, the serum samples were corrected on the seventh and fourteenth day for metabolomic study...
February 10, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28186730/engineered-hydrogen-bonded-glycopolymer-capsules-and-their-interactions-with-antigen-presenting-cells
#17
Kristian Kempe, Sue D Xiang, Paul Wilson, Md Arifur Rahim, Yi Ju, Michael R Whittaker, David M Haddleton, Magdalena Plebanski, Frank Caruso, Thomas P Davis
Hollow glycopolymer microcapsules were fabricated by hydrogen-bonded layer-by-layer (LbL) assembly, and their interactions with a set of antigen presenting cells (APCs), including dendritic cells (DCs), macrophages (MACs), and myeloid derived suppressor cells (MDSCs), were investigated. The glycopolymers were obtained by cascade postpolymerization modifications of poly(oligo(2-ethyl-2-oxazoline methacrylate)-stat-glycidyl methacrylate) involving the modification of the glycidyl groups with propargylamine and the subsequent attachment of mannose azide by copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC)...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28179901/biochemical-molecular-and-transcriptional-highlights-of-the-biosynthesis-of-an-effective-biosurfactant-produced-by-bacillus-safensis-pha3-a-petroleum-dwelling-bacteria
#18
Abdulsamie Hanano, Mouhnad Shaban, Ibrahem Almousally
Petroleum crude oil (PCO)-dwelling microorganisms have exceptional biological capabilities to tolerate the toxicity of petroleum contaminants and are therefore promising emulsifier and/or degraders of PCO. This study describes a set of PCO-inhabiting bacterial species, one of which, identified as Bacillus safensis PHA3, produces an efficient biosurfactant which was characterized as a glycolipid. Fourier transform infrared spectrometer, nuclear magnetic resonance, Thin layer chromatography, HPLC, and GC-MS analysis of the purified biosurfactant revealed that the extracted molecule under investigation is likely a mannolipid molecule with a hydrophilic part as mannose and a hydrophobic part as hexadecanoic acid (C16:0)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28179475/asparagine-linked-glycans-of-cryptosporidium-parvum-contain-a-single-long-arm-are-barely-processed-in-the-er-or-golgi-and-show-a-strong-bias-for-sites-with-threonine
#19
John R Haserick, Deborah R Leon, John Samuelson, Catherine E Costello
Cryptosporidium parvum causes severe diarrhea in infants in developing countries and in immunosuppressed persons, including those with AIDS. We are interested in the Asn-linked glycans (N-glycans) of C. parvum, because 1) the N-glycan precursor is predicted to contain five mannose and two glucose residues on a single long arm versus nine mannose and three glucose residues on the three-armed structure common in host N-glycans, 2) C. parvum is a rare eukaryote that lacks the machinery for N-glycan-dependent quality control of protein folding in the lumen of the Endoplasmic Reticulum (ER), and 3) ER and Golgi mannosidases, as well as glycosyltransferases that build complex N-glycans, are absent from the predicted proteome...
February 8, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28177792/utilization-of-carbon-sources-by-clinical-isolates-of-i-aeromonas-i
#20
Karoline de Campos Prediger, Monica Surek, Cibelle De Borba Dalagassa, Flávia Emanoelli Araújo de Assis, Mário Sérgio Piantavini, Emanuel E M Souza, Fabio O F O Pedrosa, Sônia Maria de Souza Santos Farah, Dayane Alberton, Cyntia Maria Telles Fadel-Picheth
Bacteria in the genus <i>Aeromonas</i> are primarily aquatic organisms; however, some species can cause diseases in humans, ranging from wound infections to septicemia, of which diarrhea is the most common condition. The ability to use a variety of carbon substrates is advantageous for pathogenic bacteria. Therefore, we used Biolog GN2 microplates to analyze the ability of 103 clinical, predominantly diarrheal, isolates of <i>Aeromonas</i> to use various carbon sources and verified whether among the substrates metabolized by these strains there were some endogenous to the human intestine...
December 22, 2016: Canadian Journal of Microbiology
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