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https://www.readbyqxmd.com/read/28813016/a-novel-high-mannose-specific-lectin-from-the-green-alga-halimeda-renschii-exhibits-a-potent-anti-influenza-virus-activity-through-high-affinity-binding-to-the-viral-hemagglutinin
#1
Jinmin Mu, Makoto Hirayama, Yuichiro Sato, Kinjiro Morimoto, Kanji Hori
We have isolated a novel lectin, named HRL40 from the green alga Halimeda renschii. In hemagglutination-inhibition test and oligosaccharide-binding experiment with 29 pyridylaminated oligosaccharides, HRL40 exhibited a strict binding specificity for high-mannose N-glycans having an exposed (α1-3) mannose residue in the D2 arm of branched mannosides, and did not have an affinity for monosaccharides and other oligosaccharides examined, including complex N-glycans, an N-glycan core pentasaccharide, and oligosaccharides from glycolipids...
August 16, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28810660/recent-advancements-in-understanding-mammalian-o-mannosylation
#2
M Osman Sheikh, Stephanie M Halmo, Lance Wells
The post-translational glycosylation of select proteins by O-linked mannose (O-mannose or O-man) is a conserved modification from yeast to humans and has been shown to be necessary for proper development and growth. The most well studied O-mannosylated mammalian protein is α-dystroglycan (α-DG). Hypoglycosylation of α-DG results in varying severities of congenital muscular dystrophies, cancer progression and metastasis, and inhibited entry and infection of certain arenaviruses. Defects in the gene products responsible for post-translational modification of α-DG, primarily glycosyltransferases, are the basis for these diseases...
September 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28808163/a-role-for-fimh-in-extraintestinal-pathogenic-escherichia-coli-invasion-and-translocation-through-the-intestinal-epithelium
#3
Nina M Poole, Sabrina I Green, Anubama Rajan, Luz E Vela, Xi-Lei Zeng, Mary K Estes, Anthony W Maresso
The translocation of bacteria across the intestinal epithelium of immunocompromised patients can lead to bacteremia and life-threatening sepsis. Extraintestinal Pathogenic Escherichia coli (ExPEC), so named because this pathotype infects tissues distal to the intestinal tract, is a frequent cause of such infections, is often multidrug resistant, and chronically colonizes a sizable portion of the healthy population. Although several virulence factors and their roles in pathogenesis are well described for ExPEC that cause urinary tract infections and meningitis, they have not been linked to translocation through intestinal barriers, a fundamentally distant yet important clinical phenomenon...
August 14, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28807751/a-novel-pmca3-mutation-in-an-ataxic-patient-with-hypomorphic-phosphomannomutase-2-pmm2-heterozygote-mutations-biochemical-characterization-of-the-pump-defect
#4
Mattia Vicario, Tito Calì, Domenico Cieri, Francesca Vallese, Raissa Bortolotto, Raffaele Lopreiato, Francesco Zonta, Marta Nardella, Alessia Micalizzi, Dirk J Lefeber, Enza Maria Valente, Enrico Bertini, Giuseppe Zanotti, Ginevra Zanni, Marisa Brini, Ernesto Carafoli
The neuron-restricted isoform 3 of the plasma membrane Ca(2+) ATPase plays a major role in the regulation of Ca(2+) homeostasis in the brain, where the precise control of Ca(2+) signaling is a necessity. Several function-affecting genetic mutations in the PMCA3 pump associated to X-linked congenital cerebellar ataxias have indeed been described. Interestingly, the presence of co-occurring mutations in additional genes suggest their synergistic action in generating the neurological phenotype as digenic modulators of the role of PMCA3 in the pathologies...
August 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28807689/effects-of-polysaccharides-from-abalone-viscera-haliotis-discus-hannai-ino-on-mgc-803cells-proliferation
#5
Rui-Juan Zhang, Yan Shi, Jing Zheng, Xiao-Mei Mao, Zhi-Yu Liu, Qing-Xi Chen, Qin Wang
The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment. The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively. The main monosaccharide compositions of AVP were D-galactose, D-xylose, D-mannose, D-glucose and D-glucuronic acid...
August 11, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28803976/canavalia-bonariensis-lectin-molecular-bases-of-glycoconjugates-interaction-and-antiglioma-potential
#6
Benildo Sousa Cavada, Mayara Torquato Lima Silva, Vinicius Jose Silva Osterne, Vanir Reis Pinto-Junior, Ana Paula Machado do Nascimento, Ingrid Alessandra Victoria Wolin, Isabella Aparecida Heinrich, Clareane Avelino Simplicio Nobre, Cleane Gomes Moreira, Claudia Figueiredo Lossio, Cintia Renata Costa Rocha, Jorge Luiz Martins, Kyria Santiago Nascimento, Rodrigo Bainy Leal
CaBo is a mannose/glucose-specific lectin purified from seeds of Canavalia bonariensis. In the present work, we report the CaBo crystal structure determined to atomic resolution in the presence of X-man, a specific ligand. Similar to the structural characteristics of other legume lectins, CaBo presented the jellyroll motif, a metal binding site occupied by calcium and manganese ions close to the carbohydrate-recognition domain (CRD). In vitro test of CaBo cytotoxicity against glioma cells demonstrated its ability to decrease the cellular viability and migration by induction of autophagy and cell death...
August 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28803818/a-homozygous-dpm3-mutation-in-a-patient-with-alpha-dystroglycan-related-limb-girdle-muscular-dystrophy
#7
P Y K Van den Bergh, Y Sznajer, V Van Parys, W van Tol, R A Wevers, D J Lefeber, L Xu, M Lek, D G MacArthur, K Johnson, L Phillips, A Töpf, V Straub
Defects of O-linked glycosylation of alpha-dystroglycan cause a wide spectrum of muscular dystrophies ranging from severe congenital muscular dystrophy associated with abnormal brain and eye development to mild limb girdle muscular dystrophy. We report a female patient who developed isolated pelvic girdle muscle weakness and wasting, which became symptomatic at age 42. Exome sequencing uncovered a homozygous c.131T > G (p.Leu44Pro) substitution in DPM3, encoding dolichol-P-mannose (DPM) synthase subunit 3, leading to a 50% reduction of enzymatic activity...
July 17, 2017: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/28802902/pseudomonas-aeruginosa-mannose-sensitive-hemagglutinin-injection-treated-cytokine-induced-killer-cells-combined-with-chemotherapy-in-the-treatment-of-malignancies
#8
Chaoqi Zhang, Zhen Zhang, Liping Wang, Jiaoling Han, Feng Li, Chunyi Shen, Hong Li, Lan Huang, Xuan Zhao, Dongli Yue, Jianmin Huang, Yan Yan, Yi Zhang
Pseudomonas aeruginosa-mannose sensitive hemagglutinin (PA-MSHA) injection serves as immunological adjuvant in clinical treatment of cancer patients. In present study, we investigated whether PA-MSHA injection enhanced the anti-tumor efficacy of CIK cells. Twenty patients with malignancies were enrolled in this retrospective clinical trial. They were divided into two groups: 10 patients received PA-MSHA treated CIK cells transfusion combined with chemotherapy, and other patients accepted CIK cells and chemotherapy...
August 10, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28800704/qualitative-and-quantitative-analysis-of-carbohydrate-modification-on-glycoproteins-from-seeds-of-ginkgo-biloba
#9
Ting Wang, Xiao-Chun Hu, Zhi-Peng Cai, Josef Voglmeir, Li Liu
Recent progress in the relationship between carbohydrate cross-reactive determinants (CCDs) and allergic response highlights the importance of carbohydrate moieties in innate immune system. A previous research pointed out that the protein allergen in Ginkgo biloba seeds are glycosylated and the oligosaccharides conjugated to these proteins might also contribute to the allergy. The aim of this study was to analyze carbohydrate moieties, especially the N-linked glycans, of glycoproteins from Ginkgo seeds originated from different places for detailed structures, to enable further research on the roles that N-glycans play in Ginkgo-caused allergy...
August 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28800703/mechanism-of-the-reduction-in-the-igg-and-ige-binding-of-%C3%AE-lactoglobulin-induced-by-ultrasound-pretreatment-combined-with-dry-state-glycation-a-study-using-conventional-spectrometry-and-high-resolution-mass-spectrometry
#10
Wenhua Yang, Zongcai Tu, Hui Wang, Lu Zhang, Shengsheng Xu, Chendi Niu, Honglin Yao, Igor A Kaltashov
Bovine β-lactoglobulin (β-Lg) is one of major allergens in cow's milk. Previous study showed that ultrasound treatment induced the conformational changes of β-Lg and promoted the glycation in aqueous solutions, which is, however, less efficient compared with dry-state. In this work, the effect of ultrasound pretreatment combined with dry-state glycation on the IgG and IgE binding of β-Lg was studied. Dry-state glycation with mannose after ultrasound pretreatment at 0-600 W significantly reduced the IgG and IgE binding of β-Lg, with the lowest values observed at 400 W...
August 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28797811/biochemical-characterisation-of-lectin-from-indian-hyacinth-plant-bulbs-with-potential-inhibitory-action-against-human-cancer-cells
#11
Sanjay Naik, Ravindra Singh Rawat, Santripti Khandai, Mukesh Kumar, Sidhartha S Jena, Mookambeswaran A Vijayalakshmi, Sanjit Kumar
This work describes purification and characterisation of a monocot mannose-specific lectin from Hyacinth bulbs. The purified lectin has a molecular mass of∼30kDa in reducing as well as in non-reducing SDS-PAGE. In hydrodynamic studies by Dynamic Light Scattering (DLS) showed that purified lectin was monomeric in nature with a molecular size of 2.38±0.03nm. Agglutination activity of purified lectin was confirmed by rabbit erythrocytes and its agglutination activity was inhibited by D-mannose and glycoprotein (ovalbumin)...
August 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28796447/perfluoroaryl-azide-staudinger-reaction-a-fast-and-bioorthogonal-reaction
#12
Madanodaya Sundhoro, Seaho Jeon, JaeHyeung Park, Olof Ramström, Mingdi Yan
We report a fast Staudinger reaction between perfluoroaryl azides (PFAAs) and aryl phosphines, occurring readily under ambient conditions. A rate constant as high as 18 M-1 s-1 was obtained between methyl 4-azido-2,3,5,6-tetrafluorobenzoate and methyl 2-(diphenylphosphanyl)benzoate in CD3CN/D2O. In addition, the iminophosphorane product was stable toward hydrolysis and aza-phosphonium ylide reactions. The PFAA-Staudinger reaction proved to be an excellent bioothorgonal reaction. PFAA-derivatized mannose and galactose were successfully transformed into cell surface glycans and efficiently labeled with phosphine-derivatized fluorescent bovine serum albumin...
August 10, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28795851/selective-induction-of-apoptosis-in-mcf7-cancer-cell-by-targeted-liposomes-functionalized-with-mannose-6-phosphate
#13
Cristina Minnelli, Laura Cianfruglia, Emiliano Laudadio, Roberta Galeazzi, Michela Pisani, Emanuela Crucianelli, Davide Bizzaro, Tatiana Armeni, Giovanna Mobbili
Liposomes are versatile platforms to carry anticancer drugs in targeted drug delivery; they can be surface modified by different strategies and, when coupled with targeting ligands, are able to increase cellular internalization and organelle-specific drug delivery. An interesting strategy of antitumoral therapy could involve the use of lysosomotropic ligand-targeted liposomes loaded with molecules, which can induce lysosomal membrane permeabilization (LMP), leakage of cathepsins into the cytoplasm and subsequent apoptosis...
August 10, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28795601/poly-glutamic-dendrimer-based-conjugates-for-cancer-vaccination-a-computational-design-for-targeted-delivery-of-antigens
#14
L I F Moura, N Martinho, L C Silva, T S Barata, S Brocchini, H F Florindo, M Zloh
Computational techniques are useful to predict interaction models and molecular properties for the design of drug delivery systems, such as dendrimers. This work evaluated the impact of surface modifications of mannosamine-conjugated multifunctional poly(glutamic acid) (PG)-dendrimers as nanocarriers of the tumour associated antigens (TAA) MART-1, gp100:44 and gp100:209. Molecular dynamics simulations and docking studies were performed. Nitrobenzoxadiazole (NBD)-PG-G4-dendrimer displayed 64 carboxylic groups, however, the Frontier Molecular Orbital Theory study evidenced that only 32 of those were available to form covalent bonds...
August 10, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28794230/analysis-of-factor-d-isoforms-in-malpuech-michels-mingarelli-carnevale-patients-highlights-the-role-of-masp-3-as-a-maturase-in-the-alternative-pathway-of-complement
#15
Rasmus Pihl, Lisbeth Jensen, Annette G Hansen, Ida B Thøgersen, Stephanie Andres, Frederik Dagnæs-Hansen, Konrad Oexle, Jan J Enghild, Steffen Thiel
Factor D (FD), which is also known as adipsin, is regarded as the first-acting protease of the alternative pathway (AP) of complement. It has been suggested that FD is secreted as a mature enzyme that does not require subsequent activation. This view was challenged when it was shown that mice lacking mannose-binding lectin (MBL)-associated serine protease-1 (MASP-1) and MASP-3 contain zymogenic FD (pro-FD), and it is becoming evident that MASP-3 is implicated in pro-FD maturation. However, the necessity of MASP-3 for pro-FD cleavage has been questioned, because AP activity is still observed in sera from MASP-1/3-deficient Malpuech-Michels-Mingarelli-Carnevale (3MC) patients...
August 9, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28792371/description-of-naumannella-cuiyingiana-sp-nov-isolated-from-a-ca-1500-year-old-mural-painting-and-emended-description-of-the-genus-naumannella
#16
Tian Tian, Fasi Wu, Yantian Ma, Ting Xiang, Wenxia Ma, Weigang Hu, Guangwen Wu, Lizhe An, Wanfu Wang, Huyuan Feng
Strain AFT2T was isolated from a mural painting sample from a ca. 1500-year-old tomb located in Shanxi Province, China. The isolate was a Gram-stain-positive, non-motile, non-spore-forming, aerobic and oval to short-rod-shaped bacterium that formed white-pigmented colonies. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain AFT2T was most closely (97.01 %) correlated and formed a monophyletic clade with Naumannella halotolerans WS4616T (=DSM 24323T). The G+C content of the genomic DNA was 71...
August 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28789910/one-pot-enzymatic-glycan-remodeling-of-a-therapeutic-monoclonal-antibody-by-endoglycosidase-s-endo-s-from-streptococcus-pyogenes
#17
Xin Tong, Tiezheng Li, Jared Orwenyo, Christian Toonstra, Lai-Xi Wang
A facile, one-pot enzymatic glycan remodeling of antibody rituximab to produce homogeneous high-mannose and hybrid type antibody glycoforms is described. This method was based on the unique substrate specificity of the endoglycosidase S (Endo-S) from Streptococcus pyogenes. While Endo-S efficiently hydrolyzes the bi-antennary complex type IgG Fc N-glycans, we found that Endo-S did not hydrolyze the "ground state" high-mannose or hybrid glycoforms, and only slowly hydrolyzed the highly activated high-mannose or hybrid N-glycan oxazolines...
July 29, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28783370/association-between-polymorphisms-of-the-mannose-binding-lectin-and-severity-of-periportal-fibrosis-in-schistosomiasis-in-the-northeast-of-brazil
#18
Taynan da Silva Constantino, Elker Lene Santos de Lima, Lidiane Régia Pereira Braga de Brito, Jamile Luciana Silva, Maria Rosângela Cunha Duarte Coêlho, Maria Tereza Cartaxo Muniz, Paula Carolina Valença Silva, Ana Lúcia Coutinho Domingues
AIMS: Mannose-binding lectin (MBL) is a protein synthesized by the liver and its immune response is associated with the development of liver fibrosis. We hypothesized that the polymorphisms in the Exon 1 region (52, 54, 57) and promoter regions (-550 H/L, -221 X/Y) of the MBL2 gene were associated with the severity of periportal fibrosis (PPF), and that these polymorphisms affect the MBL serum levels. MATERIALS AND METHODS: In this cross-sectional study we genotyped these polymorphisms within the MBL2 gene in 229 Brazilian subjects infected with Schistosoma mansoni, with different patterns of PPF...
August 7, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28782625/shifted-golgi-targeting-of-glycosyltransferases-and-%C3%AE-mannosidase-ia-from-giantin-to-gm130-grasp65-results-in-formation-of-high-mannose-n-glycans-in-aggressive-prostate-cancer-cells
#19
Ganapati Bhat, Vishwanath-Reddy Hothpet, Ming-Fong Lin, Pi-Wan Cheng
BACKGROUND: There is a pressing need for biomarkers that can distinguish indolent from aggressive prostate cancer to prevent over-treatment of patients with indolent tumor. METHODS: Golgi targeting of glycosyltransferases was characterized by confocal microscopy after knockdown of GM130, giantin, or both. N-glycans on a trans-Golgi enzyme β4galactosyltransferase-1 isolated by immunoprecipitation from androgen-sensitive and independent prostate cancer cells were determined by matrix-assisted laser desorption-time of flight-mass spectrometry...
August 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28782615/polyphenolic-protein-polysaccharide-ternary-conjugates-from-cystoseira-barbata-tunisian-seaweed-as-potential-biopreservatives-chemical-antioxidant-and-antimicrobial-properties
#20
Sabrine Sellimi, Abdelkarim Benslima, Veronique Barragan-Montero, Mohamed Hajji, Moncef Nasri
The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives. CBGs were successfully isolated, then chemically and structurally characterized. CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%). The carbohydrate fraction was sulfated (13.81%) and conjugated with proteins (9.86%) and phenolic compounds (4...
August 3, 2017: International Journal of Biological Macromolecules
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