Rene J M Henderikx, Daniel Mann, Aušra Domanska, Jing Dong, Saba Shahzad, Behnam Lak, Aikaterini Filopoulou, Damian Ludig, Martin Grininger, Jeffrey Momoh, Elina Laanto, Hanna M Oksanen, Kyrylo Bisikalo, Pamela A Williams, Sarah J Butcher, Peter J Peters, Bart W A M M Beulen
Single-particle cryo-electron microscopy has become a widely adopted method in structural biology due to many recent technological advances in microscopes, detectors and image processing. Before being able to inspect a biological sample in an electron microscope, it needs to be deposited in a thin layer on a grid and rapidly frozen. The VitroJet was designed with this aim, as well as avoiding the delicate manual handling and transfer steps that occur during the conventional grid-preparation process. Since its creation, numerous technical developments have resulted in a device that is now widely utilized in multiple laboratories worldwide...
April 1, 2024: Acta Crystallographica. Section D, Structural Biology