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https://www.readbyqxmd.com/read/29665961/orchestration-of-the-expression-of-the-laccase-multigene-family-in-white-rot-basidiomycete-trametes-hirsuta-072-evidences-of-transcription-level-subfunctionalization
#1
Konstantin V Moiseenko, Daria V Vasina, Katerina T Farukshina, Olga S Savinova, Olga A Glazunova, Tatiana V Fedorova, Tatiana V Tyazhelova
Laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) is an enzyme that has been studied for over 100 y and is present in virtually all fungi. As increasing numbers of fungal genomes have been sequenced, it has become apparent that the laccase genes in white-rot fungi commonly form multigene families consisting of many nonallelic genes. Although a number of reports focussing on laccase gene expression in different fungal species were published over the decades, the fundamental questions of why fungi need such a redundant array of genes and how they manage this array to perform biological function(s) remain far from answered...
May 2018: Fungal Biology
https://www.readbyqxmd.com/read/29665690/structural-characterization-of-lignin-from-maize-zea-mays-l-fibers-evidences-for-diferuloylputrescine-incorporated-into-the-lignin-polymer-in-maize-kernels
#2
José C Carlos Del Río, Jorge Rencoret, Ana Gutierrez, Hoon Kim, John Ralph
The structure of the phenolic polymer in maize grain fibers, with 5.5% Klason lignin content, has been studied. For this, the milled wood lignin (MWL) and dioxane lignin (DL) preparations were isolated and analyzed. The data indicated that the lignin in maize fibers was syringyl-rich, mostly involved in β-aryl ether, resinol and phenylcoumaran substructures. 2D-NMR and derivatization followed by reductive cleavage (DFRC) also revealed the occurrence of associated ferulates together with trace amounts of p-coumarates acylating the γ-OH of lignin side-chains, predominantly on S-lignin units...
April 18, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29665653/upregulation-of-ppar-%C3%AE-mediates-the-renoprotective-effect-of-omega-3-pufa-and-ferulic-acid-in-gentamicin-intoxicated-rats
#3
Nahla E El-Ashmawy, Naglaa F Khedr, Hoda A El-Bahrawy, Sara A Helal
Ferulic acid (FrA) is a natural product containing phenolic compounds. ω-3 PUFA is the major constituent of fish oil. The aim of this study was to investigate the renoprotective role of FrA and FO in gentamicin (GM)-induced nephrotoxicity in rats. Forty four male rats were divided equally into 4 groups: Control group, GM group, FrA + GM group and FO + GM group. Each of the treated groups was injected with GM (40 mg/kg) i.p. for 9 consecutive days. FrA (100 mg/kg) and FO (5 mL/kg) were given to rats orally daily for 10 days prior to GM and then concomitantly with GM for additional 9 days...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29662302/hot-water-extract-of-the-branches-of-hovenia-dulcis-thunb-rhamnaceae-ameliorates-low-fiber-diet-induced-constipation-in-rats
#4
Chul-Yung Choi, Seung-Sik Cho, In-Soo Yoon
Hovenia dulcis Thunb. (Rhamnaceae), also known as oriental raisin tree, is used in traditional herbal medicine. Its extracts have been reported to show various pharmacological effects such as hepatoprotection, antitumor, antiatopic dermatitis, antilipid peroxidation, anti-steatotic, anti-inflammatory, and antiallergic activities. However, there have been no reports on the effect of H. dulcis extracts in relieving constipation so far. The aim of this study was to investigate the effects of a hot-water extract of the branches of H...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29660264/selective-fragmentation-of-biore%C3%AF-nery-corncob-lignin-into-p-hydroxycinnamic-esters-with-a-supported-znmoo4-catalyst
#5
Shuizhong Wang, Wa Gao, Helong Li, Ling-Ping Xiao, Run-Cang Sun, Guoyong Song
Lignin is the largest renewable resource of bio-aromatics, and catalytic fragmentation of lignin into phenolic monomers is increasingly recognized as an important starting point for lignin valorization. Herein, we reported zinc molybdate (ZnMoO4) supported on MCM-41 can catalyze fragmentation of biorefinery technical lignin, enzymatic mild acidolysis lignin and native lignin derived from corncob, to give lignin oily products containing 15 to 37.8 wt% phenolic monomers, in which the high selectivities towards methyl coumarate 1 and methyl ferulate 2 were obtained (up to 78%)...
April 16, 2018: ChemSusChem
https://www.readbyqxmd.com/read/29659750/simultaneous-qualitative-and-quantitative-study-of-main-compounds-in-commelina-communis-linn-by-uhplc-q-tof-ms-ms-and-hplc-esi-ms-ms
#6
Xia Zhang, Caijuan Liang, Changlin Li, Mingde Bu, Liying Bu, Yuande Xiao, Hongguang Sun, Lantong Zhang
This study reported the identification and determination of the main components of Commelina communis Linn. A total of 62 compounds were identified in C. communis Linn. extract, which included 29 flavonoids and flavonoid glycosides, 17 phenolic acids, 4 alkaloids, 1 pyrimidine alkaloids, 3 sterols and 8 fatty acids and others by ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. Moreover, a specific, simple, rapid and sensitive high performance liquid chromatography with tandem mass spectrometry method was developed and validated for determination of 13 components of C...
April 11, 2018: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29655743/hyperspectral-imaging-for-non-destructive-prediction-of-fermentation-index-polyphenol-content-and-antioxidant-activity-in-single-cocoa-beans
#7
Nicola Caporaso, Martin B Whitworth, Mark S Fowler, Ian D Fisk
The aim of the current work was to use hyperspectral imaging (HSI) in the spectral range 1000-2500 nm to quantitatively predict fermentation index (FI), total polyphenols (TP) and antioxidant activity (AA) of individual dry fermented cocoa beans scanned on a single seed basis, in a non-destructive manner. Seventeen cocoa bean batches were obtained and 10 cocoa beans were used from each batch. PLS regression models were built on 170 samples. The developed HSI predictive models were able to quantify three quality-related parameters with sufficient performance for screening purposes, with external validation R2 of 0...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29642867/antiproliferative-activities-and-phenolic-acid-content-of-water-and-ethanolic-extracts-of-the-powdered-formula-of-houttuynia-cordata-thunb-fermented-broth-and-phyllanthus-emblica-linn-fruit
#8
Piyawan Kumnerdkhonkaen, Somprasong Saenglee, Md Ali Asgar, Gulsiri Senawong, Kanoknan Khongsukwiwat, Thanaset Senawong
BACKGROUND: Houttuynia cordata Thunb. and Phyllanthus emblica Linn. are native plants with medicinal and nutritive significance in Asia. The present study was aimed at evaluating antiproliferative effects on human cancer cell lines and identifying the phenolic acid composition of water and ethanolic extracts of the powdered formula of H. cordata fermented broth and P. emblica fruit. METHODS: Anticancer activity of the extracts was evaluated against HeLa, HT29, HCT116, MCF7 and Jurkat cells using an MTT assay and flow cytometric analysis of apoptosis induction and cell cycle arrest...
April 11, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29642591/light-emission-from-the-fe-2-egta-h%C3%A2-o%C3%A2-system-possible-application-for-the-determination-of-antioxidant-activity-of-plant-phenolics
#9
Michal Nowak, Wieslaw Tryniszewski, Agata Sarniak, Anna Wlodarczyk, Piotr J Nowak, Dariusz Nowak
Oxidative reactions can result in the formation of electronically excited species that undergo radiative decay depending on electronic transition from the excited state to the ground state with subsequent ultra-weak photon emission (UPE). We investigated the UPE from the Fe2+ -EGTA (ethylene glycol-bis(β-aminoethyl ether)- N , N , N ', N '-tetraacetic acid)-H₂O₂ system with a multitube luminometer (Peltier-cooled photon counter, spectral range 380 to 630 nm). The UPE of 92.6 µmol/L Fe2+ -185.2 µmol/L EGTA-2...
April 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29625941/antioxidant-capacity-and-polyphenolic-content-of-the-echinocystis-lobata-michx-torr-et-a-gray-flowers
#10
Irina Ielciu, Daniela Hanganu, Ramona Păltinean, Laurian Vlase, Michel Frédérich, Ana-Maria Gheldiu, Daniela Benedec, Gianina Crişan
Echinocystis lobata (Michx.) Torr. et A.Gray is a spontaneous species in the Romanian flora, lesser studied by scientific literature, but which has proved significant activities in traditional medicine. The present study is aimed to provide data on the polyphenolic compounds in the composition of the flowers of this species and to test their biological potential. Polyphenols were identified and quantified using an HPLC-MS method. Tested biological activities were the cytotoxic, anti-plasmodial and antioxidant ones...
March 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29619818/an-asymmetric-cationic-porphyrin-as-a-new-g-quadruplex-probe-with-wash-free-cancer-targeted-imaging-ability-under-acidic-microenvironments
#11
Ran Zhang, Meng Cheng, Li-Ming Zhang, Li-Na Zhu, Deming Kong
Porphyrins are promising candidates for nucleic acid G-quadruplex-specific optical recognition. We previously demonstrated that G-quadruplex recognition specificity of porphyrins could be improved by introducing bulky side arm substituents, but enhanced protonation tendency limits their applications in some cases, such as under acidic conditions. Here, we demonstrated that the protonation tendency of porphyrin derivatives could be efficiently overcome by increasing molecular asymmetry. To validate this, an asymmetric, water-soluble, cationic porphyrin FA-TMPipEOPP (5-{4-[2-[[(2E)-3-[3-methoxy-4-[2-(1-methyl-1- piperidinyl)ethoxy]phenyl]-1-oxo-2-propenyl]oxy]ethoxy]phenyl},10,15,20-Tri{4- [2-(1-methyl-1-piperidinyl)ethoxy]- phenyl}porphyrin) was synthesized by introducing a ferulic acid (FA) unit at one side arm, and its structure was well characterized...
April 5, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29617332/rapid-identification-of-intact-staphylococcal-bacteriophages-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#12
Dana Štveráková, Ondrej Šedo, Martin Benešík, Zbyněk Zdráhal, Jiří Doškař, Roman Pantůček
Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as a significant threat. Phage therapy could offer a safe and effective alternative to antibiotics. Phage preparations should comply with quality and safety requirements; therefore, it is important to develop efficient production control technologies. This study was conducted to develop and evaluate a rapid and reliable method for identifying staphylococcal bacteriophages, based on detecting their specific proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling that is among the suggested methods for meeting the regulations of pharmaceutical authorities...
April 4, 2018: Viruses
https://www.readbyqxmd.com/read/29617286/acetylcholinesterase-inhibition-and-antioxidant-activity-of-n-trans-caffeoyldopamine-and-n-trans-feruloyldopamine
#13
Muamer Dizdar, Danijela Vidic, Franc Požgan, Bogdan Štefane, Milka Maksimović
Phenolic acids and their derivatives found in nature are well-known for their potential biological activity. In this study, two amides derived from trans -caffeic/ferulic acid and dopamine were synthesized and characterized by Fourier-transform infrared spectroscopy (FTIR), mass spectrometry, proton and carbon-13 nuclear magnetic resonance spectroscopy. The compounds were tested for the inhibition of acetylcholinesterase (AChE) from Electrophorus electricus and for antioxidant activity by scavenging 2,2-diphenyl-1-pycrylhydrazyl free radical (DPPH• ) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (ABTS•+ ), reducing ferric ions, and ferrous ions chelation...
April 4, 2018: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/29615674/acute-metabolic-actions-of-the-major-polyphenols-in-chamomile-an-in-vitro-mechanistic-study-on-their-potential-to-attenuate-postprandial-hyperglycaemia
#14
Jose A Villa-Rodriguez, Asimina Kerimi, Laszlo Abranko, Sarka Tumova, Lauren Ford, Richard S Blackburn, Christopher Rayner, Gary Williamson
Transient hyperglycaemia is a risk factor for type 2 diabetes and endothelial dysfunction, especially in subjects with impaired glucose tolerance. Nutritional interventions and strategies for controlling postprandial overshoot of blood sugars are considered key in preventing progress to the disease state. We have identified apigenin-7-O-glucoside, apigenin, and (Z) and (E)-2-hydroxy-4-methoxycinnamic acid glucosides as the active (poly)phenols in Chamomile (Matricaria recutita) able to modulate carbohydrate digestion and absorption in vitro as assessed by inhibition of α-amylase and maltase activities...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29614031/role-of-phenolic-acids-from-the-rhizosphere-soils-of-panax-notoginseng-as-a-double-edge-sword-in-the-occurrence-of-root-rot-disease
#15
Ya-Meng Zhao, Yong-Xian Cheng, Yu-Nan Ma, Chuan-Jiao Chen, Fu-Rong Xu, Xian Dong
Chemical agents in the rhizosphere soils of plants might have an influence on root-rot disease, which therefore might reveal the mechanism of root rot in Panax notoginseng ( P. notoginseng ). With this hypothesis the alterations of phenolic acids (PAs) in the rhizosphere soils of P. notoginseng after pathogen infection were determined. The effects of PAs on the growth of Fusarium oxysporum ( F. oxysporum ), a fungal pathogenic factor for P. notoginseng , as well as production of fusaric acid, a wilting agent for the plants, were also examined...
April 3, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29606474/levels-of-terpenoids-mangiferin-and-phenolic-acids-in-the-pulp-and-peel-of-ripe-mango-fruit-influenced-by-pre-harvest-spray-application-of-feso-4-fe-2-mgso-4-mg-2-and-mnso-4-mn-2
#16
Mekhala Dinushi Kananke Vithana, Zora Singh, Stuart Keith Johnson
Fe2+ , Mg2+ and Mn2+ are enzyme cofactors in terpenoids biosynthesis. Effects of pre-harvest spray of FeSO4 , MgSO4 and MnSO4 (0.2% and 0.3%) 30 d prior to harvest on the levels of terpenoids and phenolic compounds in ripe mango fruit were investigated. All treatments significantly increased lupeol in the peel compared to control and it was highest in pulp of 0.3% FeSO4 -treated fruit. Spray of each nutrient (0.3%) increased total carotenoids in the pulp. Mangiferin in pulp was significantly higher in the fruit treated with 0...
August 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29600112/determination-of-phenolic-acids-in-sugarcane-vinasse-by-hplc-with-pulse-amperometry
#17
P V Freitas, D R da Silva, M A Beluomini, J L da Silva, N R Stradiotto
A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described. Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L-1 acetic acid (pH 5.0)/water (80 : 20, v / v ) as mobile phase under constant working potential mode of 0.80 V. Chromatographic peaks presented high resolution and separation. Calibration curves exhibited excellent correlation coefficients, above 0.995. Linear ranges of the analytes, in mg L-1 , were of 0...
2018: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/29597834/new-dianthramide-and-cinnamic-ester-glucosides-from-the-roots-of-aconitum-carmichaelii
#18
Thi Quynh Do, Bich Ngan Truong, Huong Doan Thi Mai, Thuy Linh Nguyen, Van Hung Nguyen, Hai Dang Nguyen, Tien Dat Nguyen, The Cuong Nguyen, Trieu Vung Luong, Loc Thang Giang, Van Minh Chau, Van Cuong Pham
Four new compounds N-salicyl-3-hydroxyanthranilic acid methyl ester (1), N-(2'-dehydroxysalicyl)-3-hydroxyanthranilic acid methyl ester (2), methyl-4-β-D-allopyranosyl-ferulate (3), and methyl-4-β-D-gulopyranosyl-cinnamate (4), along with six known compounds (5-10), were isolated from the roots of Aconitum carmichelii Debx. Their structures were elucidated on the basis of spectral data analysis, including 1D, 2D-NMR, and HR-ESI-MS. Compounds 1 and 2 showed the inhibition of nitric oxide (NO) production with IC50 values of 9...
March 30, 2018: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/29587452/investigation-of-linum-flavum-l-hairy-root-cultures-for-the-production-of-anticancer-aryltetralin-lignans
#19
Sullivan Renouard, Cyrielle Corbin, Samantha Drouet, Barbara Medvedec, Joël Doussot, Cyril Colas, Benoit Maunit, Avninder S Bhambra, Eric Gontier, Nathalie Jullian, François Mesnard, Michèle Boitel, Bilal Haider Abbasi, Randolph R J Arroo, Eric Lainé, Christophe Hano
Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated...
March 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29580515/probiotic-potential-of-yeasts-isolated-from-pineapple-and-their-use-in-the-elaboration-of-potentially-functional-fermented-beverages
#20
Juliana Cunha Amorim, Roberta Hilsdorf Piccoli, Whasley Ferreira Duarte
Pineapple (Ananas comosus (L.) Merril) is a tropical fruit rich in nutrients characterized by a pleasant taste and widely consumed in several countries. It is used to produce juice, jams and wine. In this work, 150 yeasts isolates were obtained from peel (18) and spontaneously fermented pineapple pulp (132). The probiotic potential of 50 isolates was studied. Survival at pH 2.0, pepsin 3.0 g/L, and tolerance of bile salts (0.1 and 1% (w/v) were determined as indicators of survival potential of the isolates during the passage through the human gastrointestinal tract in simulated conditions...
May 2018: Food Research International
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