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VZV whole blood

Marieke van der Heiden, Lia G H de Rond, Menno C van Zelm, Guy A M Berbers, Annemieke M H Boots, Anne-Marie Buisman
Background: Prevention of infectious diseases is of high priority in the rapidly aging population. Unfortunately, vaccine responses in the elderly are frequently diminished. Timely vaccination of middle-aged adults might improve the immune responses to vaccines, although knowledge on pathogen-specific immune responses and factors affecting these responses, in middle-aged adults is currently limited. We thus investigated the immune responses after vaccination with Zostavax consisting of live-attenuated varicella zoster virus (VZV)...
2018: Frontiers in Immunology
Natsuko Inazawa, Tsukasa Hori, Masanori Nojima, Makoto Saito, Keita Igarashi, Masaki Yamamoto, Norio Shimizu, Yuko Yoto, Hiroyuki Tsutsumi
Several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-HSCT) are frequent, but viral reactivations after autologous HSCT (auto-HSCT) have not been investigated in detail. We performed multiplex polymerase chain reaction (PCR) assay to examine multiple viral reactivations simultaneously in 24 patients undergoing auto-HSCT between September 2010 and December 2012. Weekly whole blood samples were collected from pre- to 42 days post-HSCT, and tested for the following 13 viruses; herpes simplex virus 1 (HSV-1), HSV-2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6), HHV-7, HHV-8, adeno virus (ADV), BK virus (BKV), JC virus (JCV), parvovirus B19 (B19V), and hepatitis B virus (HBV)...
February 2017: Journal of Medical Virology
Daniel F Hoft, Azra Blazevic, Asmir Selimovic, Aldin Turan, Jan Tennant, Getahun Abate, John Fulkerson, Daniel E Zak, Robert Walker, Bruce McClain, Jerry Sadoff, Judy Scott, Barbara Shepherd, Jasur Ishmukhamedov, David A Hokey, Veerabadran Dheenadhayalan, Smitha Shankar, Lynn Amon, Garnet Navarro, Rebecca Podyminogin, Alan Aderem, Lew Barker, Michael Brennan, Robert S Wallis, Anne A Gershon, Michael D Gershon, Sharon Steinberg
BACKGROUND: We report a first-in-human trial evaluating safety and immunogenicity of a recombinant BCG, AERAS-422, over-expressing TB antigens Ag85A, Ag85B, and Rv3407 and expressing mutant perfringolysin. METHODS: This was a randomized, double-blind, dose-escalation trial in HIV-negative, healthy adult, BCG-naïve volunteers, negative for prior exposure to Mtb, at one US clinical site. Volunteers were randomized 2:1 at each dose level to receive a single intradermal dose of AERAS-422 (>10(5)-<10(6)CFU=low dose, ≥10(6)-<10(7)CFU=high dose) or non-recombinant Tice BCG (1-8×10(5)CFU)...
May 2016: EBioMedicine
Cindy Mourgues, Cecile Henquell, Zuzana Tatar, Bruno Pereira, Cinthya Nourisson, Anne Tournadre, Martin Soubrier, Marion Couderc
INTRODUCTION: IL-6 is involved in viral immunosurveillance. We studied the effect of tocilizumab (TCZ) on the evolution in viral load (VL) for the Epstein-Barr virus (EBV), cytomegalovirus (CMV) and varicella-zoster virus (VZV) in patients with rheumatoid arthritis (RA). METHODS: EBV, CMV and VZV loads were prospectively determined in whole blood of 22 RA patients at TCZ initiation and during treatment follow-up. A difference of 0.5 log10 or of threefold copies/mL between two VL was considered significant...
July 2016: Joint, Bone, Spine: Revue du Rhumatisme
Natsuko Inazawa, Tsukasa Hori, Naoki Hatakeyama, Masaki Yamamoto, Yuko Yoto, Masanori Nojima, Nobuhiro Suzuki, Norio Shimizu, Hiroyuki Tsutsumi
Viral reactivations following hematopoietic stem cell transplantation are thought to result from the breakdown of both cell-mediated and humoral immunity. As a result, many viruses could be reactivated individually or simultaneously. Using a multiplex polymerase chain reaction (PCR), we prospectively examined many kinds of viral DNAs at a time in 105 patients who underwent allogeneic hematopoietic stem cell transplantation. In total, 591 whole blood samples were collected weekly from pre- to 42 days post-transplantation and the following 13 viruses were tested; herpes simplex virus 1 (HSV-1), HSV-2, varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus 6 (HHV-6), HHV-7, HHV-8, adenovirus, BK virus (BKV), JC virus (JCV), parvovirus B19, and hepatitis B virus (HBV)...
August 2015: Journal of Medical Virology
C Meyer, J Walker, J Dewane, F Engelmann, W Laub, S Pillai, Charles R Thomas, I Messaoudi
In this study we examined the effects of non-myeloablative total body irradiation (TBI) in combination with immunosuppressive chemotherapy on immune homeostasis in rhesus macaques. Our results show that the administration of cyclosporin A or tacrolimus without radiotherapy did not result in lymphopenia. The addition of TBI to the regimen resulted in lymphopenia as well as alterations in the memory/naive ratio following reconstitution of lymphocyte populations. Dendritic cell (DC) numbers in whole blood were largely unaffected, while the monocyte population was altered by immunosuppressive treatment...
September 2015: Clinical and Experimental Immunology
Xiaoli Wang, Xiaoyan Li, Shuhong Hu, Hongmei Qu, Yinghong Zhang, Huijie Ni, Xiaoliang Wang
Understanding the association between congenital human cytomegalovirus (HCMV) infection and active maternal HCMV infection during pregnancy is important for maternal and neonatal healthcare. In the present study, a loop-mediated isothermal amplification (LAMP) method was established for the detection of CMV DNA from whole blood or amniotic fluid samples, using reverse transcription-quantitative polymerase chain reaction. The results of the present study demonstrated that the CMV LAMP assay detection was specific for CMV DNA, whereas it did not detect viral DNA from herpes simplex type 1 (HSV-1), HSV-2, varicella zoster virus, HSV-6 or HSV-7...
August 2015: Molecular Medicine Reports
Mosaad Abdel-Aziz, Noha A Azab, Badwy Khalifa, Mohammed Rashed, Nader Naguib
OBJECTIVES: Bell's palsy is considered the most common cause of facial nerve paralysis in children. Although different theories have been postulated for its diagnosis, reactivation of the Varicella zoster virus (VZV) has been implicated as one of the causes of Bell's palsy. The aim of the study was to evaluate the association of Varicella-zoster virus infection with Bell's palsy and its outcome in children. METHODS: A total of 30 children with Bell's palsy were recruited and were assayed for evidence of VZV infection...
March 2015: International Journal of Pediatric Otorhinolaryngology
Christine Meyer, Flora Engelmann, Nicole Arnold, David L Krah, Jan ter Meulen, Kristen Haberthur, Jesse Dewane, Ilhem Messaoudi
UNLABELLED: Varicella-zoster virus (VZV) is a human neurotropic alphaherpesvirus and the etiological agent of varicella (chickenpox) and herpes zoster (HZ, shingles). Previously, inoculation of monkeys via the subcutaneous, intratracheal, intravenous, or oral-nasal-conjunctival routes did not recapitulate all the hallmarks of VZV infection, including varicella, immunity, latency, and reactivation. Intrabronchial inoculation of rhesus macaques (RMs) with simian varicella virus (SVV), a homolog of VZV, recapitulates virologic and immunologic hallmarks of VZV infection in humans...
February 2015: Journal of Virology
D K Bayer, C A Martinez, H S Sorte, L R Forbes, G J Demmler-Harrison, I C Hanson, N M Pearson, L M Noroski, S R Zaki, W J Bellini, M S Leduc, Y Yang, C M Eng, A Patel, O K Rodningen, D M Muzny, R A Gibbs, I M Campbell, C A Shaw, M W Baker, V Zhang, J R Lupski, J S Orange, F O Seeborg, A Stray-Pedersen
In areas without newborn screening for severe combined immunodeficiency (SCID), disease-defining infections may lead to diagnosis, and in some cases, may not be identified prior to the first year of life. We describe a female infant who presented with disseminated vaccine-acquired varicella (VZV) and vaccine-acquired rubella infections at 13 months of age. Immunological evaluations demonstrated neutropenia, isolated CD4 lymphocytopenia, the presence of CD8(+) T cells, poor lymphocyte proliferation, hypergammaglobulinaemia and poor specific antibody production to VZV infection and routine immunizations...
December 2014: Clinical and Experimental Immunology
Tomasz Dzieciatkowski, Maciej Przybylski, Patrycja Rusicka, Krzysztof Madry, Piotr Boguradzki, Halina Marchel, Wiesław W Jedrzejczak, Grazyna Młynarczyk
INTRODUCTION: Infections caused with a variety of bacteria, fungi and viruses are still responsible for high level of mortality and morbidity in immunosupressed individuals. A case of fatal post-transplant reactivation with four herpesviruses in 49-year-old immunocompromised male with MDS-RAEB2, subjected to allogeneic haematopoietic stem cell transplantation was described. METHODS: Full microbiological examination of was performed in different types of clinical samples (whole blood, stool)...
2014: Medycyna Doświadczalna i Mikrobiologia
Ross Sadler, Elizabeth A L Bateman, Victoria Heath, Smita Y Patel, Phillip P Schwingshackl, Alice C Cullinane, Lisa Ayers, Berne L Ferry
BACKGROUND: Clinical investigation of antigen-specific T cells in potentially immunodeficient patients is an important and often challenging aspect of patient diagnostic work up. Methods for detection of microbial exposure to the T-cell compartment exist but are laborious and time consuming. Recently, a whole blood technique involving flow cytometry and detection of CD25 and OX40 (CD134) expression on the surface of activated CD4+ T cells was shown to be accurate and concordant when compared with more traditional methods of antigen-specific T-cell detection...
September 2014: Cytometry. Part B, Clinical Cytometry
Ross Sadler, Elizabeth Al Bateman, Victoria Heath, Smita Y Patel, Phillip P Schwingshackl, Alice C Cullinane, Lisa Ayers, Berne L Ferry
Background: Clinical investigation of antigen-specific T cells in potentially immunodeficient patients is an important and often challenging aspect of patient diagnostic work up. Methods for detection of microbial exposure to the T cell compartment exist but are laborious and time consuming. Recently, a whole blood technique involving flow cytometry and detection of CD25 and OX40 (CD134) expression on the surface of activated CD4+ T cells was shown to be accurate and concordant when compared with more traditional methods of antigen-specific T cell detection...
February 11, 2014: Cytometry. Part B, Clinical Cytometry
Christine Meyer, Jesse Dewane, Kristen Haberthur, Flora Engelmann, Nicole Arnold, Wayne Gray, Ilhem Messaoudi
BACKGROUND: Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that infects humans and results in chickenpox and herpes zoster. A number of VZV genes remain functionally uncharacterized and since VZV is an obligate human pathogen, rigorous evaluation of VZV mutants in vivo remains challenging. Simian varicella virus (SVV) is homologous to VZV and SVV infection of rhesus macaques (RM) closely mimics VZV infection of humans. Recently the SVV genome was cloned as a bacterial artificial chromosome (BAC) and BAC-derived SVV displayed similar replication kinetics as wild-type (WT) SVV in vitro...
2013: Virology Journal
Cheryl S Toi, Meav-Lang J Lay, Robyn Lucas, Choo Beng Chew, Janette Taylor, Anne-Louise Ponsonby, Dominic E Dwyer
Primary infection with varicella zoster virus (VZV) occurs in immunocompromised and immunocompetent individuals. Clinical and asymptomatic reactivation with shedding of infectious virus and viremia may occur. The prevalence of VZV viremia is unknown. The aim of this study was to detect VZV viremia and quantify VZV DNA using quantitative polymerase chain reaction (qPCR) in blood from different populations. A qPCR-based method using EvaGreen® was used to quantify VZV DNA in 491 samples, including whole blood, plasma and buffy-coat, from patients hospitalized with varicella-associated disease (Group 1, n=10) and three groups with no VZV disease: individuals with a first clinical diagnosis of central nervous system demyelination (Group 2, n=213) with their age and sex-matched controls (Group 3, n=218); and HIV-infected individuals (Group 4, n=50)...
August 2013: Journal of Medical Virology
Dai Watanabe, Naruhito Otani, Sachiko Suzuki, Hiromi Dohi, Kazuyuki Hirota, Hitoshi Yonemoto, Yusuke Koizumi, Hiroshi Otera, Keishiro Yajima, Yasuharu Nishida, Tomoko Uehira, Masayuki Shima, Takuma Shirasaka, Toshiomi Okuno
The development of herpes zoster is associated with reduced varicella zoster virus (VZV)-specific cell-mediated immune (CMI) reactions. In this study, VZV-specific CMI reactions in 42 anti-VZV-IgG antibody-positive adults infected with HIV-1 were evaluated by measuring the IFN-γ production levels in whole blood in response to stimulation with ultraviolet light-inactivated live attenuated VZV vaccine. The median VZV-specific IFN-γ production level in all patients was 63 pg/ml. Antiretroviral therapy (ART)-naïve patients with an AIDS-defining illness (HIV classification category C) had significantly lower IFN-γ production than ART-naïve patients in categories A and B and patients receiving ART (P=0...
August 2013: Journal of Medical Virology
Szymon Kierat, Katarzyna Leś, Maciej Przybylski, Tomasz Dzieciatkowski, Grazyna Młynarczyk
INTRODUCTION: Varicella-zoster virus (HHV-3) is spread by the respiratory route and disseminates to lymph nodes and then via lymph back to the skin, resulting with the rash of chickenpox. Like other alpha-herpesviruses, HHV-3 infects the neurons of the dorsal root ganglia, where it causes lifelong latency. Virus reactivation causes episode of herpes zoster (shingles). During severe HHV-3 infections molecular methods, such as real-time PCR (qPCR) assay, are recognized as a method-of-choice for detecting viral DNA in clinical specimens...
2012: Medycyna Doświadczalna i Mikrobiologia
Andreas Sauerbrei, Anna Schäfler, Jörg Hofmann, Michael Schacke, Bernd Gruhn, Peter Wutzler
Commercial serologic assays for varicella-zoster virus (VZV), which enable reliable determination of VZV immune status and are amenable to automation, are needed. The present study compares the automated performance of the VZV whole-cell enzyme-linked immunosorbent assay (ELISA) Enzygnost anti-VZV/IgG, the Euroimmun anti-VZV ELISA (IgG) based on highly purified viral proteins, and the VZV glycoprotein (gp)-based Serion ELISA Classic VZV IgG. The fluorescent-antibody-to-membrane-antibody (FAMA) test was used as a reference...
August 2012: Clinical and Vaccine Immunology: CVI
Bing Chen, Min Zhou, Jian Ouyang, Rongfu Zhou, Jingyan Xu, Qiguo Zhang, Yonggong Yang, Yong Xu, Xiaoyan Shao, Li Meng, Jing Wang, Yun Xu, Xiushi Ni, Xueguang Zhang
Autologous haematopoietic stem cell transplantation (AHSCT) is a promising treatment for multiple sclerosis (MS) patients who have not adequately responded to conventional therapies. We retrospectively evaluated the safety and long-term clinical outcome of AHSCT in MS patients in China. Twenty-five patients with various types of MS were treated with AHSCT. Peripheral blood stem cells were derived by leukapheresis after mobilized with granulocyte colony-stimulating factor. Then CD34+ cell selection of the graft was performed and anti-thymocyte globulin was given for T-cell depletion, with the conditioning regimen BEAM adopted and early and late toxicities recorded...
August 2012: Neurological Sciences
Nadia Ben Fredj, Antonella Rotola, Faten Nefzi, Saber Chebel, Roberta Rizzo, Elisabetta Caselli, Mahbouba Frih-Ayed, Dario Di Luca, Mahjoub Aouni
Members of the human Herpesviridae family are candidates for representing the macroenvironmental factors associated with multiple sclerosis (MS) pathogenesis. To verify the possible role of human herpesviruses (HHVs) as triggering or aggravating factors in relapsing-remitting multiple sclerosis clinical outcome, we studied the prevalence of all eight human herpesviruses in whole blood samples collected from 51 MS patients and from 51 healthy controls. The presence of DNA of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), human herpesvirus 6 (HHV-6), human herpesvirus 7 (HHV-7) and human herpesvirus 8 (HHV-8) was searched by specific nested polymerase chain reaction...
February 2012: Journal of Neurovirology
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