Xujia Wu, Huairui Yuan, Qiulian Wu, Yixin Gao, Tingting Duan, Kailin Yang, Tengfei Huang, Shuai Wang, Fanen Yuan, Derrick Lee, Suchet Taori, Tritan Plute, Søren Heissel, Hanan Alwaseem, Michael Isay-Del Viscio, Henrik Molina, Sameer Agnihotri, Dennis J Hsu, Nu Zhang, Jeremy N Rich
Cancers commonly reprogram translation and metabolism, but little is known about how these two features coordinate in cancer stem cells. Here we show that glioblastoma stem cells (GSCs) display elevated protein translation. To dissect underlying mechanisms, we performed a CRISPR screen and identified YRDC as the top essential transfer RNA (tRNA) modification enzyme in GSCs. YRDC catalyzes the formation of N6 -threonylcarbamoyladenosine (t6 A) on ANN-decoding tRNA species (A denotes adenosine, and N denotes any nucleotide)...
March 22, 2024: Nature Cancer