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https://www.readbyqxmd.com/read/29781072/ascites-index-a-novel-technique-to-evaluate-ascites-in-ovarian-hyperstimulation-syndrome-a-concept-proof-study
#1
Piotr Robert Szkodziak, Piotr Czuczwar, Wojciech Wrona, Tomasz Paszkowski, Filip Szkodziak, Sławomir Woźniak
OBJECTIVES: Controlled ovarian hyperstimulation is an important step in infertility treatment. In some cases, however, ovar-ian hyperstimulation syndrome (OHSS) can occur. In its severe forms, ascites is likely to develop, associated with dyspnea. The aim of this study was to explore the usefulness of Ascites Index (AsI), a new tool for quantitative determination of ascites in patients with OHSS, to obtain data for planning further trials. MATERIAL AND METHODS: Twelve patients with OHSS and ascites were included in the study...
2018: Ginekologia Polska
https://www.readbyqxmd.com/read/29779470/ascites-derived-extracellular-micrornas-as-potential-biomarkers-for-ovarian-cancer
#2
Luděk Záveský, Eva Jandáková, Vít Weinberger, Luboš Minář, Veronika Hanzíková, Daniela Dušková, Lenka Záveská Drábková, Iveta Svobodová, Aleš Hořínek
Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, large-scale expression profiling of 754 human miRNAs was performed using real-time quantitative polymerase chain reaction and 384-well TaqMan array human miRNA A and B cards to identify differentially expressed miRNAs between extracellular fraction of the ascitic fluid associated with high-grade serous ovarian carcinomas and control plasma...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29767833/post-operative-care-of-interventional-therapy-for-40-liver-cancer-patients-with-obstructive-jaundice
#3
De-Ping Tong, Li-Qin Wu, Xiao-Ping Chen, Yi Li
The care of 40 patients with primary liver cancer with obstructive jaundice treated with liver puncture bile drainage or biliary stent implantation was reported. Treated with the interventional therapy, patients were observed closely to identify symptoms of hepatic encephalopathy and pain; diet care was well performed. Bile drainage tube and skin acre were performed carefully. Liver function, bilirubin and other biochemical indicators were monitored; occurrence of bleeding, acute pancreatitis, biliary tract infection, leakage of ascites around drainage tube and other complication were observed with good discharge instruction...
May 16, 2018: European Journal of Cancer Care
https://www.readbyqxmd.com/read/29767242/in-vivo-antitumor-activity-of-liposome%C3%A2-plasmid-dna-encoding-mutant-survivin%C3%A2-t34a-in-cervical-cancer
#4
Fang Qiu, Xia Zhao
The aim of the present study was to investigate the influence of liposome‑plasmid encoding mutant survivin‑T34A (PST34A) on tumor growth in cervical cancer in vivo. Liposome‑plasmid DNA encoding mutant survivin‑T34A was constructed and administered via an intraperitoneal injection in mice inoculated with cervical cancer cells. Following the establishment of the tumor model, the animals were randomly divided into four groups: i) The normal saline group (NS; 100 µl sterile saline once/3 days for 15 days); ii) the 1,2‑dioleoyl‑3‑trimethylammonium‑propane (DOTAP) control (100 µg DOTAP once/3 days for 15 days); iii) the plasmid PST34A (10 µg PST34A once/3 days for 15 days); and iv) the PST34A+DOTAP (10 µg PST34A+100 µg DOTAP once/3 days for 15 days)...
May 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29755076/-constrictive-pericarditis-with-intractable-ascites-after-aortic-valve-replacement-report-of-a-case
#5
Rie Kageyama, Kyouhei Ueno, Masatoshi Sunada, Hirofumi Midorikawa, Megumu Kanno, Takashi Takano, Kouichi Satou
We report a 68-year-old man, who developed refractory ascites of unknown cause after aortic valve replacement. He was diagnosed with constrictive pericarditis because of "dip-and-plateau" waveform findings via cardiac catheterization and operated with cardiopulmonary bypass. Following waffle procedure, we incised pericardium for decompression, so that pericardial mobility and diastolic dysfunction was improved. Postoperative computed tomography (CT) image also showed decrease of ascites fluid. We concluded that pericardiotomy is an established surgical procedure and is excellent indication to constrictive pericarditis...
March 2018: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/29753914/fluorescence-correlation-spectroscopy-to-find-the-critical-balance-between-extracellular-association-and-intracellular-dissociation-of-mrna-complexes
#6
Heyang Zhang, Stefaan C De Smedt, Katrien Remaut
Fluorescence Correlation Spectroscopy (FCS) is a promising tool to study interactions on a single molecule level. The diffusion of fluorescent molecules in and out of the excitation volume of a confocal microscope leads to the fluorescence fluctuations that give information on the average number of fluorescent molecules present in the excitation volume and their diffusion coefficients. In this context, we complexed mRNA into lipoplexes and polyplexes and explored the association/dissociation degree of complexes by using gel electrophoresis and FCS...
May 10, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29744812/analytical-scheme-leading-to-integrated-high-sensitivity-profiling-of-glycosphingolipids-together-with-n-and-o-glycans-from-one-sample
#7
John D Benktander, Solomon T Gizaw, Stefan Gaunitz, Milos V Novotny
Glycoconjugates are directly or indirectly involved in many biological processes. Due to their complex structures, the structural elucidation of glycans and the exploration of their role in biological systems have been challenging. Glycan pools generated through release from glycoprotein or glycolipid mixtures can often be very complex. For the sake of procedural simplicity, many glycan profiling studies choose to concentrate on a single class of glycoconjugates. In this paper, we demonstrate it feasible to cover glycosphingolipids, N-glycans, and O-glycans isolated from the same sample...
May 9, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29743795/prevalence-and-impact-of-bacterial-infections-in-children-with-liver-disease-a-prospective-study
#8
Rishi Bolia, Anshu Srivastava, Rungmei Marak, Surender K Yachha, Ujjal Poddar
Background and aims: Risk of infections is increased in patients with Acute Liver Failure (ALF) and Decompensated Chronic Liver Disease (DCLD). We evaluated the frequency, site, type and risk-factors for bacterial infections in children with ALF and DCLD and its effect on outcome. Methods: ALF or DCLD children were enrolled prospectively. Clinical and laboratory details were recorded. Cultures (blood, urine and ascites) and chest X-ray were done at admission followed by weekly surveillance cultures...
March 2018: Journal of Clinical and Experimental Hepatology
https://www.readbyqxmd.com/read/29736145/functional-activity-of-natural-killer-cells-in-biological-fluids-in-patients-with-colorectal-and-ovarian-cancers
#9
Natalia V Yunusova, Marina N Stakheyeva, Sergey V Molchanov, Sergey G Afanas'ev, Anastasia A Tsydenova, Larisa A Kolomiets, Nadejda V Cherdyntseva
Aim of the study: To compare the functional activity of natural killer cells depending on the presence of a malignant process and its dissemination. Material and methods: The study included 20 patients with Stage IIIB, C (FIGO, 2009) ovarian cancer, 10 patients with benign ovarian tumours (BOT), and 20 patients with colorectal cancer (T2-4N0-2M0). The control group consisted of 9 healthy donors. To evaluate the number and functional activity of NK cells, multicolour flow cytometry was performed...
2018: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/29728076/effective-preparation-of-a-monoclonal-antibody-against-human-chromogranin-a-for-immunohistochemical-diagnosis
#10
Danping Zhang, Chengjie Xie, Rongzhi Wang, Qinghai Yang, Huiling Chen, Sumei Ling, Shihua Wang, Kunzhi Jia
BACKGROUND: Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive tumor as a biomarker. RESULTS: The synthesized gene of CgA coding area was cloned and expressed as fusion protein CgA-His in procaryotic system. Then the purified CgA-His protein was mixed with QuickAntibody-Mouse5W adjuvant, and injected into mice...
May 4, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29727353/role-of-survivin-and-p53-expression-in-response-of-primary-culture-of-ovarian-cancer-cells-to-treatment-with-chemotherapeutic-agents
#11
Diwesh Chawla, Rajarshi Kar, Bindiya Gupta, Sumita Halder, Seema Garg, Mohit Mehndiratta, Neelam Wadhwa, Rachna Agarwal
BACKGROUND: Ovarian cancer is associated with a high relapse rate and is the fifth leading cause of cancer deaths in women. The genetic profile of a tumor is responsible for deciding response to chemotherapeutic agents. In this study, we investigate the relation between survivin and p53 expression and response to chemotherapeutic agents of primary cultures of ovarian cancer cells established from ascitic fluid. MATERIALS AND METHOD: Ascitic fluid and Dulbecco's modified Eagle medium was mixed in equal proportion in culture flasks and incubated to establish primary culture...
April 30, 2018: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/29717278/role-of-obesity-in-the-release-of-extracellular-nucleosomes-in-acute-pancreatitis-a-clinical-and-experimental-study
#12
Salvador Pérez, Isabela Finamor, Pablo Martí-Andrés, Javier Pereda, Ana Campos, Rosário Domingues, Fawaz Haj, Luis Sabater, Enrique de-Madaria, Juan Sastre
BACKGROUND/OBJECTIVES: A high body mass index increases the risk of severe pancreatitis and associated mortality. Our aims were: (1) To determine whether obesity affects the release of extracellular nucleosomes in patients with pancreatitis; (2) To determine whether pancreatic ascites confers lipotoxicity and triggers the release of extracellular nucleosomes in lean and obese rats. METHODS: DNA and nucleosomes were determined in plasma from patients with mild or moderately severe acute pancreatitis either with normal or high body mass index (BMI)...
May 1, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29702988/enteritis-as-initial-manifestation-of-systemic-lupus-erythematosus-in-early-pregnancy-a-case-report
#13
Anna Maria Bellou, Dominik Bös, Guido Kukuk, Ulrich Gembruch, Waltraut Maria Merz
RATIONAL: Lupus enteritis is a rare, severe complication of systemic lupus erythematosus (SLE). We report of a patient who presented with enteritis as manifestation of new-onset SLE during the first trimester of pregnancy. PATIENTS CONCERNS: The 23-year nulliparous patient was admitted to a district hospital with abdominal pain, nausea, vomiting and bloody diarrhea at a gestational age (GA) of 10 weeks. Her symptoms improved with symptomatic treatment and she was discharged a few days later...
April 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29701519/chylous-ascites-associated-with-abdominal-trauma-and-intestinal-resection-anastomosis-in-a-pet-ferret-mustela-putorius-furo
#14
Lucile Chassang, Isabelle Langlois, Pauline Loos, Mila Freire, Elizabeth O'Toole
CASE DESCRIPTION A 10-week-old 0.73-kg (1.6-lb) castrated male domestic ferret (Mustela putorius furo) was referred for exploratory laparotomy because of pneumoperitoneum and possible septic peritonitis after being bitten by the owner's dog. CLINICAL FINDINGS Abdominal exploration revealed a large laceration of the duodenum, tears of the jejunal mesentery, and 2 small tears in the abdominal wall. Chylous abdominal effusion developed 48 hours after surgery. TREATMENT AND OUTCOME Postoperative care included supportive treatment, analgesia, and antimicrobials...
May 15, 2018: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/29701391/diagnostic-challenges-in-chronic-constrictive-pericarditis
#15
Ana Braga, Afonso Oliveira, Kevin Domingues, Maria João Andrade, Miguel Abecacis, José Pedro Neves
INTRODUCTION: Chronic constrictive pericarditis (CCP) is a disease that has multiple possible causes and is associated with variable clinical findings, depending on its severity. It develops insidiously, and in many cases, particularly in developed countries, no antecedent diagnosis can be found. These cases are termed idiopathic. Tuberculosis is the leading cause of constrictive pericarditis in developing nations but represents only a small minority in developed countries. METHODS: Here the authors describe two different case reports where tuberculosis was the probable cause of CCP...
July 2017: Revista Portuguesa de Cirurgia Cardio-torácica e Vascular
https://www.readbyqxmd.com/read/29695699/-novel-glycyrrhetinic-acid-derivative-soloxolone-methyl-inhibits-the-inflammatory-response-and-tumor-growth-in-vivo
#16
A V Markov, A V Sen'kova, M A Zenkova, E B Logashenko
Due to wide spreading of inflammatory disease and imperfection of available anti-inflammatory drugs, mainly associated with their serious side effects, searching for new anti-inflammatory agents is a pressing problem. Natural triterpenoids and their synthetic analogs are a promising source of new drugs. In this study, we have investigated the anti-inflammatory and antitumor effects in vivo of the glycyrrhetinic acid derivative soloxolone methyl (SM), or methyl 2-cyano-3,12-dioxo-18βH-olean-9(ll),l(2)-dien-30-oate...
March 2018: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/29688031/gynecologic-serous-carcinoma-an-immunohistochemical-analysis-of-malignant-body-fluid-specimens
#17
Shuyue Ren, William Klump
CONTEXT: - Evaluation of fluid specimens involved by serous carcinoma might potentially include PAX8, GATA3, Uroplakin II, SOX2, and SALL4 antibodies. Those markers are commonly employed for diagnosing carcinomas of various types, including urothelial malignancies and germ cell tumors. There have been no comprehensive immunohistochemical studies, to our knowledge, for those markers on fluid specimens involved by serous carcinoma. OBJECTIVE: - To evaluate immunohistochemical markers PAX8, GATA3, SOX2, uroplakin II, and SALL4 in the diagnosis of high-grade serous carcinoma in fluid specimens...
April 24, 2018: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/29685110/wirsung-atraumatic-rupture-in-patient-with-pancreatic-pseudocysts-a-case-presentation
#18
Martino Gerosa, Marco Chiarelli, Angelo Guttadauro, Matilde De Simone, Fulvio Tagliabue, Melchiorre Costa, Sabina Terragni, Ugo Cioffi
BACKGROUND: Pancreatic duct disruption is a challenging condition leading to pancreatic juice leakage and consequently to pancreatic fluid collections. The manifestations of pancreatic main duct leak include pseudocysts, walled-off necrosis, pancreatic fistulas, ascites, pleural and pericardial effusions. Pseudocyst formation is the most frequent outcome of a pancreatic duct leak. CASE PRESENTATION: We describe a case of a 64-year old man with large multiple pancreatic cysts discovered for progressive jaundice and significant weight loss in the absence of a previous episode of acute pancreatitis...
April 23, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29684847/the-association-between-p53-protein-phosphorylation-at-serine-15-serine-20-and-sensitivity-of-cells-isolated-from-patients-with-ovarian-cancer-and-cell-lines-to-chemotherapy-in-in-vitro-study
#19
Iwona Kamińska, Julia K Bar
BACKGROUND: The association between p53 protein phosphorylated at serine 15 (Ser15), serine 20 (Ser20) and ovarian tumor cell sensitivity after chemotherapy was analyzed in order to define the influence of p53 activation on tumor cell sensitivity to chemotherapy. METHODS: The study was performed on ovarian cancer cell line (OvBH-1), colon adenocarcinoma metastasis to ovary (SW626) and on cells isolated from ascitic fluids from patients with ovarian cancer: with (p53+) or without (p53-) p53 nuclear protein accumulation...
December 15, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29684369/alpha-fetoprotein-in-pericardial-peritoneal-and-pleural-fluids-a-body-fluid-matrix-evaluation
#20
William E Owen, Joshua J H Hunsaker, Jonathan R Genzen
OBJECTIVES: Alpha-fetoprotein (AFP) measurement in pericardial, peritoneal (ascites), and pleural fluids is sometimes requested by clinicians as supportive evidence in the evaluation of suspected malignancy. As commercially available, Food and Drug Administration (FDA)-cleared AFP assays are not validated for these fluid types, laboratories must complete additional validation studies to comply with regulatory requirements for body fluid testing. The objective of this study was therefore to conduct a matrix evaluation for these body fluid types using the Beckman Access AFP assay on the UniCel DxI 800 immunoassay system...
April 20, 2018: Clinical Biochemistry
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