Read by QxMD icon Read


Jin-Lun Lai, Yu-Hui Liu, Chang Liu, Ming-Pu Qi, Rui-Ning Liu, Xi-Fang Zhu, Qiu-Ge Zhou, Ying-Yu Chen, Ai-Zhen Guo, Chang-Min Hu
Indirubin plays an important role in the treatment of many chronic diseases and exhibits strong anti-inflammatory activity. However, the molecular mode of action during mastitis prophylaxis remains poorly understood. In this study, a lipopolysaccharide (LPS)-induced mastitis mouse model showed that indirubin attenuated histopathological changes in the mammary gland, local tissue necrosis, and neutrophil infiltration. Moreover, indirubin significantly downregulated the production of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α)...
October 7, 2016: Inflammation
Zhu-Ling Guo, Des R Richardson, Danuta S Kalinowski, Zaklina Kovacevic, Kian Cheng Tan-Un, Godfrey Chi-Fung Chan
BACKGROUND: Neuroblastoma is a relatively common and highly belligerent childhood tumor with poor prognosis by current therapeutic approaches. A novel anti-cancer agent of the di-2-pyridylketone thiosemicarbazone series, namely di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT), demonstrates promising anti-tumor activity. Recently, a second-generation analogue, namely di-2-pyridylketone 4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC), has entered multi-center clinical trials for the treatment of advanced and resistant tumors...
September 27, 2016: Journal of Hematology & Oncology
Ching-Yi Tsai, Faith C H Li, Carol H Y Wu, Alice Y W Chang, Samuel H H Chan
BACKGROUND: Small ubiquitin-related modifier (SUMO) is a group of proteins that participates in post-translational modifications. One known SUMO target is the transcription factor nuclear factor-kB (NF-kB) that plays a pivotal role in many disease processes; sumoylation inactivates NF-kB by conjugation with inhibitors of NF-kB (IkB). Our laboratory demonstrated previously that transcriptional upregulation of nitric oxide synthase II (NOS II) by NF-kB, leading to nitrosative stress by the formation of peroxynitrite in the rostral ventrolateral medulla (RVLM), underpins the defunct brain stem cardiovascular regulation that precedes brain death...
2016: Journal of Biomedical Science
Sébastien Pinte, Bertrand Caetano, Alexandra Le Bras, Chantal Havet, Gaëlle Villain, Racha Dernayka, Catherine Duez, Virginie Mattot, Fabrice Soncin
Activation of the blood vessel endothelium is a critical step during inflammation. Endothelial cells stimulated by pro-inflammatory cytokines play an essential part in the adhesion and extravasation of circulating leucocytes into inflamed tissues. The endothelial egfl7 gene (VE-statin) represses endothelial cell activation in tumors, and prior observations suggested that it could also participate in the regulation of endothelial cell activation during inflammation. We show here that Egfl7 expression is strongly repressed in mouse lung endothelial cells during LPS- and TNFα-induced inflammation in vivo...
September 20, 2016: Journal of Biological Chemistry
Valérie Lang, Elisa Da Silva-Ferrada, Rosa Barrio, James D Sutherland, Manuel S Rodriguez
SUMO-interacting motifs (SIMs) recognize SUMOylated proteins with high specificity allowing to connect SUMO-modified proteins. Multiple SIMs fused to distinct tags have been used to increase their affinity and generate more efficient purification tools. Enrichment of SUMOylated proteins using SIMs arranged in tandem (SUMO-traps) facilitates the identification and characterization of protein targets in vitro and in vivo. Here a protocol to produce biotinylated SUMO-traps (bioSUBEs) to capture SUMO chains and typical SUMOylated proteins such as p53 or IkBα is presented...
2016: Methods in Molecular Biology
Giulia Castellani, Giovanna Paliuri, Genny Orso, Nicola Paccagnella, Claudio D'Amore, Laura Facci, Francesca Cima, Federico Caicci, Pietro Palatini, Sergio Bova, Sara De Martin
Thymic epithelial cells (TECs) play a key role in the regulation of central immune tolerance by expressing autoantigens and eliminating self-reactive T cells. In a previous paper we reported that adrenomedullin (ADM) and its co-receptor protein RAMP2 are located intracellularly in newborn human thymic epithelial cells (TECs). This work has two main aims: (1) to examine the cellular localization of ADM and its receptor in TECs of adult Wistar rats to validate this animal model for the study of the ADM system and its function(s) in thymus; (2) to investigate the potential modulating effect of ADM on the NF-kB pathway, which is involved through the production of cytokines such as IL-6, in the maturation of T-lymphocytes and immunological tolerance...
December 2016: Cytokine
Stefanie Trojandt, Iris Bellinghausen, Angelika B Reske-Kunz, Matthias Bros
Immature dendritic cells (iDCs) and tolerogenic DCs are essential for the induction and maintenance of peripheral tolerance. Tumors produce immuno-modulatory factors which imprint a pro-tolerogenic, maturation-resistant state in DCs. Here we asked for common markers of differentially tolerized human monocyte-derived DC populations. For this, PBMC-derived monocytes were differentiated to DCs in the presence of established immuno-modulators as released by tumors (IL-6, IL-10, TGF-ß, glucocorticoid [GC], prostaglandin E2 [PGE2])...
September 1, 2016: Human Immunology
Jeremy R Townsend, Jeffrey R Stout, Adam R Jajtner, David D Church, Kyle S Beyer, Leonardo P Oliveira, Michael B La Monica, Joshua J Riffe, Tyler W D Muddle, Kayla M Baker, David H Fukuda, Michael D Roberts, Jay R Hoffman
PURPOSE: The NF-κB signaling pathway regulates multiple cellular processes following exercise stress. This study aims to examine the effects of an acute lower-body resistance exercise protocol and subsequent recovery on intramuscular NF-κB signaling. METHODS: Twenty-eight untrained males were assigned to either a control (CON; n = 11) or exercise group (EX; n = 17) and completed a lower-body resistance exercise protocol consisting of the back squat, leg press, and leg extension exercises...
August 31, 2016: European Journal of Applied Physiology
Lang Cao, Rongtao Li, Xuanqin Chen, Yong Xue, Dan Liu
Neougonin A is a prenylated flavonoid isolated from the whole plants of Helminthostachys zeylanica (Ophioglossaceae), which was usually used as traditional Chinese herbal medicine for the treatment of fever and inflammation. In this study, the pharmacological effects and underlying molecular mechanisms of neougonin A on lipopolysaccharide (LPS)-induced inflammatory responses were investigated. We observed that neougonin A reduced the production of inflammatory mediators (TNFα, PGE2, NO, IL-1β, and IL-6; P < 0...
August 31, 2016: Inflammation
Cui Yang, Zhengtao Li, Saimei Yan, Yonghui He, Rong Dai, George Pek-Heng Leung, Shitian Pan, Jinyan Yang, Rong Yan, Guanhua Du
BACKGROUND AND PURPOSE: Vascular inflammation is a major factor contributing to the development of vascular diseases. The aim of this study was to investigate the role of α3-containing nicotinic acetylcholine receptors subtype (α3-nAChRs) in vascular inflammation. EXPERIMENTAL APPROACH: Vascular inflammation was studied in the apolipoprotein E knock-out (ApoE(-/-) ) mice fed with a high-fat diet. Inflammatory markers were detected in mouse aortic endothelial cells (MAECs) and macrophages after α3-nAChRs were antagonized pharmacologically or after the gene of α3-nAChRs was silenced...
August 29, 2016: British Journal of Pharmacology
Jian-Ming Zhou, Sha-Sha Gu, Wang Hong Mei, Jun Zhou, Zhen Zhong Wang, Wei Xiao
Ginkgolide and bilobalide are major trilactone constituent of Ginkgo biloba leaves and have been shown to exert powerful neuroprotective properties. The aims of this study were to observe the inhibitory effects of ginkgolide and bilobalide on the activation of microglial cells induced by oxygen-glucose deprivation and reoxygenation (OGD/R) and the specific mechanisms by which these effects are mediated. For detecting whether ginkgolide and bilobalide increased cell viability in a dose-dependent manner, BV2 cells were subjected to oxygen-glucose deprivation for 4 h followed by 3 h reoxygenation with various concentrations of drugs (6...
August 25, 2016: Cell Stress & Chaperones
Maria Lauda Tomasi, Komal Ramani, Minjung Ryoo
Lipopolysaccharide (LPS), a bacterial endotoxin, induces inflammation in macrophages via activation of NF-κB signaling. Sumoylation is a post-translational modification mediated by the small ubiquitin-like modifier, SUMO. Ubiquitin-conjugating enzyme 9 (UBC9) is the only known SUMO conjugating enzyme. LPS treatment lowers SUMO-1 and UBC9 mRNA levels in primary astrocytes. UBC9 can degrade NF-κB inhibitor α (Ikbα) via a SUMO2/3-ubiquitin pathway. However, UBC9 may also promote Ikbα stability by SUMO-1 conjugation that further regulates NF-κB signaling...
September 2016: American Journal of Pathology
Marcella Reale, Jole Fiorito
BACKGROUND: MRJF4, a novel haloperidol metabolite II prodrug, was obtained through the esterification of the secondary hydroxyl group of haloperidol metabolite II with 4-phenylbutyric acid. The activities of (±)-MRJF4 and its two enantiomers [(+)-MRJF4 and (-)-MRJF4] as tumor specific inducers of pro-apoptotic genes were evaluated on malignant C6 glioma cells. In particular, changes in Nf-kB signaling pathway, activity of nitric oxide synthases (NOS), metalloproteinases (MMPs), and membrane adhesion proteins were investigated...
August 23, 2016: Central Nervous System Agents in Medicinal Chemistry
Herwig P Moll, Andy Lee, Clayton R Peterson, Jesus Revuelta Cervantes, Brandon M Wojcik, Anshul Parulkhar, Alessandra Mele, Philip J LoGerfo, Jeffrey J Siracuse, Eva Csizmadia, Cleide G da Silva, Christiane Ferran
BACKGROUND: Inflammation is central to the pathogenesis of transplant arteriosclerosis (TA). We questioned whether physiologic levels of anti-inflammatory A20 influence TA severity. METHODS: We performed major histocompatibility complex (MHC) mismatched aorta to carotid artery interposition grafts, using wild type (WT) or A20 heterozygote (HET) C57BL/6 (H-2) donors and BALB/c (H-2) recipients, and conversely BALB/c donors and WT/HET recipients. We analyzed aortic allografts by histology, immunohistochemistry, immunofluorescence, and gene profiling (qPCR)...
August 3, 2016: Transplantation
Jian Liu, Danqing Li, Xuying Sun, Yuting Wang, Qiangbing Xiao, Anmin Chen
Bacteria-induced inflammatory responses cause excessive bone resorption in chronic inflammatory diseases such as septic arthritis, osteomyelitis, and orthopedic implant failure. Icariine has been reported to facilitate the bone healing and reduce the occurrence of osteoporosis in clinical, moreover, laboratory studies which have proved that Icariine promotes the proliferation and differentiation of osteoblasts in vitro. The present study aimed to evaluate the effects of Icariine on lipopolysaccharide (LPS)-induced bone loss via an osteogenic-in vitro model and to elucidate the underlying molecular mechanisms...
October 2016: Inflammation
Raman Saggu, Toni Schumacher, Florian Gerich, Cordula Rakers, Khalid Tai, Andrea Delekate, Gabor C Petzold
Vascular cognitive impairment is the second most common form of dementia. The pathogenic pathways leading to vascular cognitive impairment remain unclear but clinical and experimental data have shown that chronic reactive astrogliosis occurs within white matter lesions, indicating that a sustained pro-inflammatory environment affecting the white matter may contribute towards disease progression. To model vascular cognitive impairment, we induced prolonged mild cerebral hypoperfusion in mice by bilateral common carotid artery stenosis...
2016: Acta Neuropathologica Communications
Shun-Tian Liang, Yuan Li, Xiao-Wu Li, Jun-Jiang Wang, Fu-Xian Tan, Qin-Rui Han, Lu Li, Xue-Qing Yao, Xue-Gang Sun
OBJECTIVE: To investigate the effects of telocinobufagin on viability and apoptosis of colorectal cancer (CRC) cells and explore the mechanism of telocinobufagin-induced apoptosis. METHODS: MTT assay was performed to detect the viability of CRC cells exposed to telocinobufagin. Nuclear staining with Hoechst 33342 and flow cytometry were used to analyze the cell death of CRC cells. Expressions of proteins related with cell apoptosis and oxidative stress were determined with Western blotting...
June 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Shuyun Guo, Yanwu Liu, Rui Ma, Jun Li, Binxiao Su
Increasing evidence has demonstrated the role of endogenous cannabinoids system (ECS) on protecting brain injury caused by ischemia (IMI). Papers reported that microglia-mediated inflammation has become one of the most pivotal mechanisms for IMI. This study was aimed to investigate the potential roles of ECS on neuron protection under microglia-mediated inflammation. Inflammatory cytokines level both in vitro (BV-2 cells) and in vivo (brain tissue from constructed IMI model and brain-isolated microglia) was detected...
2016: American Journal of Translational Research
Jing-Jing Yang, Peng Li, Fu Wang, Wen-Jing Liang, Hui Ma, Yuan Chen, Zhi-Min Ma, Quan-Zhong Li, Qi-Sheng Peng, Yun Zhang, Shuang-Xi Wang
AIMS: Aspirin has been used for the secondary prevention and treatment of cardiovascular disease for several decades. We investigated the roles of transcriptional factor activator protein 2α (AP-2α) in the beneficial effects of aspirin in the growth and vulnerability of atherosclerotic plaque. METHODS AND RESULTS: In mice deficient of apolipoprotein E (Apoe-/-), aspirin (20, 50 mg/kg/day) suppressed the progression of atherosclerosis in aortic roots and increased the plaque stability in carotid atherosclerotic plaques induced by collar-placement...
July 4, 2016: Oncotarget
Wang Yan, Weinv Fan, Caijing Chen, Yunqin Wu, Zhenyi Fan, Jiaqi Chen, Zhaoying Chen, Huimin Chen
This study was aimed to research the effects of IL-15 on inducing inflammatory infiltration of macrophages in polymyositis (PM) through the NF-kB pathway, and whether IL-15 was able to further regulate MMP-9 expression levels. Prepared PM cells, collected from the patients suffering from PM, were administered to SD rats. Also, a group of healthy SD rats was undergoing the same treatment as the control group. The test animals were treated with either anti-IL-15, IL-15, MMP-9 siRNA or ERK1/2 inhibitor. The blood toxicological parameters creatine kinase (CK) and CD163 were tested by using ELISA and immunohistochemistry assay...
October 10, 2016: Gene
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"