keyword
MENU ▼
Read by QxMD icon Read
search

Stepping

keyword
https://www.readbyqxmd.com/read/28941410/the-contribution-of-human-non-human-animal-chimeras-to-stem-cell-research
#1
REVIEW
Sonya Levine, Laura Grabel
Chimeric animals are made up of cells from two separate zygotes. Human/non-human animal chimeras have been used for a number of research purposes, including human disease modeling. Pluripotent stem cell (PSC) research has relied upon the chimera approach to examine the developmental potential of stem cells, to determine the efficacy of cell replacement therapies, and to establish a means of producing human organs. Based on ethical issues, this work has faced pushback from various sources including funding agencies...
September 14, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28941404/development-of-a-simple-multi-residue-determination-method-of-80-veterinary-drugs-in-oplegnathus-punctatus-by-liquid-chromatography-coupled-to-quadrupole-orbitrap-mass-spectrometry
#2
Fei Zhao, Xin Gao, Zhixu Tang, Xin Luo, Miaomiao Wu, Jiachao Xu, Xiaoting Fu
A simple, rapid and sensitive multi-residue analytical method was developed and validated for 80 veterinary drugs in Oplegnathus punctatus using ultrahigh performance liquid chromatography-Orbitrap high resolution mass spectrometry (LC-HRMS). The analytes belong to 12 different families include benzimidazoles, β-lactams, lincosamides, macrolides, nitromidazoles, quinolones, sulfonamides and trimethoprim, tetracyclines, triphenylmethane dyes, amphenicols, nonsteroidal estrogens and steroid hormones. The sample preparation was optimized base on QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure...
September 18, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28938744/an-improved-ivm-method-for-cumulus-oocyte-complexes-from-small-follicles-in-polycystic-ovary-syndrome-patients-enhances-oocyte-competence-and-embryo-yield
#3
F Sánchez, F Lolicato, S Romero, M De Vos, H Van Ranst, G Verheyen, E Anckaert, J E J Smitz
STUDY QUESTION: Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence of C-Type Natriuretic Peptide (CNP) followed by a maturation step in presence of FSH and Amphiregulin (AREG)? SUMMARY ANSWER: A strategy involving prematuration culture (PMC) in the presence of CNP followed by IVM using FSH + AREG increases oocyte maturation potential leading to a higher availability of Day 3 embryos and good-quality blastocysts for single embryo transfer...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938442/angiotensin-ii-causes-beta-cell-dysfunction-through-an-er-stress-induced-pro-inflammatory-response
#4
Stanley M H Chan, Yeh-Siang Lau, Alyson A Miller, Jacqueline M Ku, Simon Potocnik, Ji-Ming Ye, Owen L Woodman, Terence P Herbert
The metabolic syndrome is associated with an increase in the activation of renin angiotensin system (RAS) and inhibition of RAS reduces the incidence of new onset diabetes. Importantly, angiotensin II (AngII), independently of its vasoconstrictor action causes beta-cell inflammation and dysfunction, which may be an early step in the development of type-2 diabetes. The aim of this study was to determine how AngII causes beta cell dysfunction. Islets of Langerhans were isolated from C57BL/6J mice that had been infused with AngII in the presence or absence of taurine-conjugated ursodeoxycholic acid (TUDCA) and effects on ER stress, inflammation and beta cell function determined...
August 17, 2017: Endocrinology
https://www.readbyqxmd.com/read/28938389/shortening-and-angulation-strategies-to-address-composite-bone-and-soft-tissue-defects
#5
Sarah N Pierrie, Joseph R Hsu
Gustilo-Anderson IIIB tibial fractures and infected tibial nonunions represent particular challenges for the orthopaedic trauma surgeon. Debridement of dysvascular bone and soft tissues can create composite bone and soft tissue defects. Restoring the soft tissue envelope is a critical step in preventing or treating ongoing infection and in restoring local blood supply. Shortening and angulation techniques, including the gradual expansion muscle flap, rely on distraction histogenesis to address composite bone and soft tissue loss...
October 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28938375/difficult-implant-removals
#6
Amy Voedisch, Michele Hugin
PURPOSE OF REVIEW: Subdermal contraceptive implant insertions have rapidly increased worldwide. These devices are usually removed 3-5 years after insertion. Although removals are generally straightforward, difficult removals can occur. This review discusses the overall approach to potentially problematic removals and the appropriate steps to avoid complications and safely remove implants. RECENT FINDINGS: Given the rapid uptake of contraceptive implants and subsequent need for removals, an Implants Removal Task Force was formed to create standard protocols for difficult implant removals worldwide...
September 21, 2017: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28938328/bulla-down-endoscopic-sphenoidotomy
#7
Mohannad Al-Qudah
Surgical approach to the sphenoid sinus is still one of the most challenging steps in sinus surgery. The author describes a novel, simple, and safe technique to access sphenoid sinus to manage isolated sphenoid pathologies or approach skull base by removing the bulla ethmoidalis and temporarily lateralizing the middle turbinate during endoscopic sinus surgery. The current approach provides an effective method to access the sphenoid sinus without increasing morbidity and avoids unnecessary possible complications associated with turbinates' resection...
September 21, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28938313/a-novel-3-step-method-for-correction-of-type-1-stahl-ear
#8
Mimi R Borrelli, Edward H Davidson, Anand R Kumar
BACKGROUND: Stahl ear, a rare congenital auricular anomaly, occurs when a third crus crosses the scaphoid fossa. Optimal elimination of the third crus and reconstruction the superior crus in type 1 Stahl ear remain undercharacterized. We present a novel 3-step technique to reliably correct a type 1 Stahl ear as a case report. METHODS: A 10-year-old male with bilateral prominent type 1 Stahl ears is presented. An anterior curvilinear helical fold incision and posterior V wedge excision was used for exposure...
September 21, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28938232/best-practice-guidelines-for-the-measurement-of-physical-activity-levels-in-stroke-survivors-a-secondary-analysis-of-an-observational-study
#9
Louise Tinlin, Natalie Fini, Julie Bernhardt, Lucy K Lewis, Tim Olds, Coralie English
The aim of this study was to investigate the phenomenon of activity (increased activity in response to monitor wear) and determine the minimum wear time of accelerometers when objectively measuring habitual physical activity levels of community dwelling stroke survivors. Exploratory, secondary analyses of cross-sectional data were carried out. Physical activity variables [sitting, standing and stepping time, step count, light physical activity and moderate-to-vigorous physical activity (MVPA)] were measured with two activity monitors for 7 days...
September 21, 2017: International Journal of Rehabilitation Research. Revue Internationale de Recherches de Réadaptation
https://www.readbyqxmd.com/read/28938126/the-need-for-theory-evaluation-in-global-citizenship-programmes-the-case-of-the-gcsa-programme
#10
Sarah Goodier, Carren Field, Suki Goodman
Many education programmes lack a documented programme theory. This is a problem for programme planners and evaluators as the ability to measure programme success is grounded in the plausibility of the programme's underlying causal logic. Where the programme theory has not been documented, conducting a theory evaluation offers a foundational evaluation step as it gives an indication of whether the theory behind a programme is sound. This paper presents a case of a theory evaluation of a Global Citizenship programme at a top-ranking university in South Africa, subsequently called the GCSA Programme...
August 24, 2017: Evaluation and Program Planning
https://www.readbyqxmd.com/read/28938077/one-step-for-a-hospital-ten-steps-for-women-african-american-women-s-experiences-in-a-newly-accredited-baby-friendly-hospital
#11
Elizabeth M Miller, Adetola F Louis-Jacques, Tara F Deubel, Ivonne Hernandez
BACKGROUND: Despite strides made by the Baby-Friendly Hospital Initiative to improve and normalize breastfeeding, considerable racial inequality persists in breastfeeding rates. Few studies have explored African American women's experience in a Baby-Friendly Hospital Initiative system to understand sources of this inequality. Research aim: This study aimed to explore African American women's experiences of the Ten Steps to Successful Breastfeeding at a women's center associated with a university-affiliated hospital that recently achieved Baby-Friendly status...
September 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/28938068/direct-patterning-of-zinc-sulfide-on-a-sub-10-nanometer-scale-via-electron-beam-lithography
#12
Mohammad S M Saifullah, Mohamed Asbahi, Maryam Binti-Kamran Kiyani, Sudhiranjan Tripathy, Esther A H Ong, Asadullah Ibn Saifullah, Hui Ru Tan, Tanmay Dutta, Ramakrishnan Ganesan, Suresh Valiyaveettil, Karen Siew-Ling Chong
Nanostructures of metal sulfides are conventionally prepared via chemical techniques and patterned using self-assembly. This poses a considerable amount of challenge when arbitrary shapes and sizes of nanostructures are desired to be placed at precise locations. Here we describe an alternative approach of nanoscale patterning of zinc sulfide (ZnS) directly using a spin-coatable and electron beam-sensitive zinc butylxanthate resist without the lift-off or etching step. Time-resolved electron beam damage studies using micro-Raman and micro-FTIR spectroscopies suggest that exposure to a beam of electrons lead to quick disappearance of xanthate moieties most likely via the Chugaev elimination, and further increase of electron dose results in the appearance of ZnS, thereby making the exposed resist insoluble in organic solvents...
September 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28938062/news-from-the-editors-roberto-romero-editor-of-ultrasound-in-obstetrics-and-gynecology-from-2003-to-2017-steps-down
#13
Stuart Campbell
No abstract text is available yet for this article.
September 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28938017/a-highly-conserved-amino-acid-in-vp1-regulates-maturation-of-enterovirus-71
#14
Yong-Xin Zhang, Yu-Ming Huang, Quan-Jie Li, Xiao-Yu Li, Yong-Dong Zhou, Fei Guo, Jin-Ming Zhou, Shan Cen
Enterovirus 71 (EV71) is the major causative agent of hand, foot and mouth disease (HFMD) in children, causing severe clinical outcomes and even death. Here, we report an important role of the highly conserved alanine residue at position 107 in the capsid protein VP1 (VP1A107) in the efficient replication of EV71. Substitutional mutations of VP1A107 significantly diminish viral growth kinetics without significant effect on viral entry, expression of viral genes and viral production. The results of mechanistic studies reveal that VP1A107 regulates the efficient cleavage of the VP0 precursor during EV71 assembly, which is required, in the next round of infection, for the transformation of the mature virion (160S) into an intermediate or A-particle (135S), a key step of virus uncoating...
September 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28937917/head-impact-exposure-measured-in-a-single-youth-football-team-during-practice-drills
#15
Mireille E Kelley, Joeline M Kane, Mark A Espeland, Logan E Miller, Alexander K Powers, Joel D Stitzel, Jillian E Urban
OBJECTIVE This study evaluated the frequency, magnitude, and location of head impacts in practice drills within a youth football team to determine how head impact exposure varies among different types of drills. METHODS On-field head impact data were collected from athletes participating in a youth football team for a single season. Each athlete wore a helmet instrumented with a Head Impact Telemetry (HIT) System head acceleration measurement device during all preseason, regular season, and playoff practices...
September 12, 2017: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/28937911/characterization-and-application-of-the-enzyme-peroxidase-to-the-degradation-of-the-mycotoxin-don
#16
Ana Carla P Feltrin, Sabrina De O Garcia, Sergiane S Caldas, Ednei G Primel, Eliana Badiale-Furlong, Jaqueline Garda-Buffon
Deoxynivalenol (DON), one of the main mycotoxins found in food matrices, has high level of toxicity. This study aimed to characterize the peroxidase enzyme extracted from rice bran to be applied to the biodegradation of DON in order to evaluate the potential peroxidase (PO) from rice bran (RB) has to degrade DON in optimal conditions. Purification and recovery factors of PO extracted from RB and purified by three-phase partitioning were 5.7% and 50%, respectively. PO had the highest level of activity in the phosphate buffer 5 mM pH 5...
September 22, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28937899/microbiological-evaluation-of-ozone-on-dentinal-lesions-in-young-permanent-molars-using-the-stepwise-excavation
#17
Osama Safwat, Mona Elkateb, Karin Dowidar, Hala Abdel Salam, Omar El Meligy
AIM: To assess the microbial effect of ozone gas on dentinal lesions in young permanent molars using the stepwise excavation. STUDY DESIGN: An experimental, controlled clinical trial was performed. The sample included 80 immature first permanent molars, showing deep occlusal carious cavities that were indicated for stepwise excavation. Following first step of dentin excavation, the sample was divided into test (ozone gas) and control (calcium hydroxide (Ca(OH)2) base material) groups...
September 22, 2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28937854/probiotics-from-isolation-to-application
#18
Parisa Shokryazdan, Mohammad Faseleh Jahromi, Juan Boo Liang, Yin Wan Ho
Probiotics have become highly recognized as supplements for humans and animals because of their beneficial effects on health and well-being. The present review aims to provide an overview of different steps through which microbial strains become applicable probiotics in food and/or feed industries. Isolation of potential probiotic strains is the first step. Lactic acid bacteria are the most frequently used microorganisms as probiotics, which can be isolated from human, animal, plant, and environment. The next steps are identification of the isolates and characterization of them based on the main selection criteria for any potential probiotic microorganism, including resistance to gastric acidity and bile salt, adherence to mucus and/or intestinal epithelial cells and cell lines, and antimicrobial and antagonism activity against potentially pathogenic microbes...
September 22, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28937809/effects-of-clonal-reproduction-on-evolutionary-lag-and-evolutionary-rescue
#19
Maria E Orive, Michael Barfield, Carlos Fernandez, Robert D Holt
Evolutionary lag-the difference between mean and optimal phenotype in the current environment-is of keen interest in light of rapid environmental change. Many ecologically important organisms have life histories that include stage structure and both sexual and clonal reproduction, yet how stage structure and clonality interplay to govern a population's rate of evolution and evolutionary lag is unknown. Effects of clonal reproduction on mean phenotype partition into two portions: one that is phenotype dependent, and another that is genotype dependent...
October 2017: American Naturalist
https://www.readbyqxmd.com/read/28937776/4-c-methoxy-2-deoxy-2-fluoro-modified-ribonucleotides-improve-metabolic-stability-and-elicit-efficient-rnai-mediated-gene-silencing
#20
Elise Malek-Adamian, Dale Guenther, Shigeo Matsuda, Saúl Martínez-Montero, Ivan Zlatev, Joel Harp, Mihai Burai Patrascu, Donald J Foster, Johans J Fakhoury, Lydia Perkins, Nicolas Moitessier, Rajar Manoharan, Nate Taneja, Anna Bisbe, Klaus Charisse, Martin A Maier, Kallanthottathil G Rajeev, Martin Egli, Muthiah Manoharan, Masad J Damha
We designed novel 4'-modified 2'-deoxy-2'-fluorouridine (2'-F U) analogues with the aim to improve nuclease resistance and potency of therapeutic siRNAs by introducing 4'-C-methoxy (4'-OMe) as the alpha (C4'α) or beta (C4'β) epimers. The C4'α epimer was synthesized by a stereoselective route in six steps; however, both α and β epimers could be obtained by a non-stereoselective approach starting from 2'-F U. (1)H-NMR analysis and computational investigation of the α-epimer revealed that the 4'-OMe imparts a conformational bias towards the North-East sugar pucker, due to intramolecular hydrogen bonding and hyperconjugation effects...
September 22, 2017: Journal of the American Chemical Society
keyword
keyword
52392
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"