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miR-34a

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https://www.readbyqxmd.com/read/28089712/effect-of-heme-oxygenase-1-on-ochratoxin-a-induced-nephrotoxicity-in-mice
#1
Agnieszka Loboda, Anna Stachurska, Paulina Podkalicka, Mateusz Sobczak, Olga Mucha, Agnieszka Witalisz-Siepracka, Alicja Jozkowicz, Jozef Dulak
Heme oxygenase-1 (HO-1), a heme-degrading enzyme, is suggested to play an important role in kidney pathophysiology, mostly due to its anti-fibrotic, anti-apoptotic and anti-oxidant properties. One of the mycotoxin, ochratoxin A (OTA) was previously shown to affect HO-1 expression, however, the mechanisms of OTA-induced nephrotoxicity during HO-1 deficiency are unknown. We have shown that OTA regulates the number of pro-fibrotic, pro-inflammatory, anti-oxidative and pro-apoptotic factors in HO-1 dependent manner, as the lack of HO-1 accelerates whereas the induction of HO-1 expression by cobalt protoporphyrin (CoPP) attenuates nephrotoxic effect of OTA...
January 9, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28086873/cecal-micrornaome-response-to-salmonella-enterica-serovar-enteritidis-infection-in-white-leghorn-layer
#2
Guixian Wu, Yukai Qi, Xiaoyi Liu, Ning Yang, Guiyun Xu, Liying Liu, Xianyao Li
BACKGROUND: Salmonella enterica serovar Enteritidis (SE) is a food-borne pathogen and of great threat to human health through consuming the contaminated poultry products. MicroRNAs (miRNAs) play an important role in different biological activities and have been shown to regulate the innate immunity in the bacterial infection. The objective of this study is to identify miRNAs associated with SE infection in laying chicken cecum. RESULTS: Average number of reads of three libraries constructed from infected and non-infected chickens was 12,476,156 and 10,866,976, respectively...
January 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28073349/the-mir-34a-5p-promotes-the-multi-chemoresistance-of-osteosarcoma-via-repression-of-the-agtr1-gene
#3
Youguang Pu, Fangfang Zhao, Yinpeng Li, Mingda Cui, Haiyan Wang, Xianghui Meng, Shanbao Cai
BACKGROUND: Chemoresistance hinders the curative cancer chemotherapy. MicroRNAs (miRNAs) are key players in diverse biological processes including the chemoresistance of cancers. METHODS: A RNA-seq-based miR-omic analysis of osteosarcoma (OS) cells was performed to detect the levels of miR-34a-5p. Bioinformatics analysis revealed that AGTR1 is one of the target genes of miR-34a-5p. The mRNA and protein levels of AGTR1 were detected in both the miR-34a-5p-mimic transfected G-292 and miR-34a-5p-antagomiR transfected SJSA-1 cells...
January 10, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28043137/pegylated-cationic-vectors-containing-a-protease-sensitive-peptide-as-a-mirna-delivery-system-for-treating-breast-cancer
#4
Ye Zeng, Zixuan Zhou, Minmin Fan, Tao Gong, Zhirong Zhang, Xun Sun
Several targeted drug delivery systems have recently been developed to increase the bioavailability of a drug at its site of action, allowing simultaneous reduction of the total necessary drug dose as well as side effects. Here, we designed a cationic gene vector containing matrix metalloproteinase-2 (MMP2)-cleavable substrate peptides that specifically target tumor sites where MMP2 levels are high. The targeted delivery system is fabricated by linking enzyme-cleavable polyethylene glycol (PEG) derivatives to cationic β-cyclodextrin-polyethylenimine conjugates, which reduce the toxicity of polyethylenimine and condense the therapeutic cargo...
January 3, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28035390/influence-of-microrna%C3%A2-34a-on-proliferation-invasion-and-metastasis-of-hct116-cells
#5
Chunyan Li, Shuming Lu, Yingyan Wang, Shibin Guo, Tianyu Zhao, Xiaosheng Wang, Bo Song
The aim of the present study was to investigate the role of microRNA (miR)‑34a expression in the proliferation, invasion and metastasis of colon cancer and its underlying mechanisms. HCT116 cells were cultured in high‑sugar Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum and 1000 U/ml penicillin‑streptomycin. Following digestion and resuspension, the cells were used for transfection, expression and western blot analysis. HCT116 cells from miR‑34a transfection, negative control and blank control groups were seeded into a 96‑well plate at a density of 105 cells/ml, and 200 µl complete DMEM was added...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28017891/self-immolative-nanoparticles-for-simultaneous-delivery-of-microrna-and-targeting-of-polyamine-metabolism-in-combination-cancer-therapy
#6
Ying Xie, Tracy Murray-Stewart, Yazhe Wang, Fei Yu, Jing Li, Laurence J Marton, Robert A Casero, David Oupický
Combination of anticancer drugs with therapeutic microRNA (miRNA) has emerged as a promising anticancer strategy. However, the promise is hampered by a lack of desirable delivery systems. We report on the development of self-immolative nanoparticles capable of simultaneously delivering miR-34a mimic and targeting dysregulated polyamine metabolism in cancer. The nanoparticles were prepared from a biodegradable polycationic prodrug, named DSS-BEN, which was synthesized from a polyamine analog N(1),N(11)-bisethylnorspermine (BENSpm)...
December 23, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28010900/emergence-of-mir-34a-in-radiation-therapy
#7
REVIEW
Jerome Lacombe, Frederic Zenhausern
Expressions of many microRNAs (miRNAs) in response to ionizing radiation (IR) have already been investigated and some of them seem to play an important role in the tumor radioresistance, normal tissue radiotoxicity or as predictive biomarkers to radiation. miR-34a is an emerging miRNA in recent radiobiology studies. Here, we review this miR-34 family member by detailing its different roles in radiation response and we will discuss about the role that it can play in radiation treatment. Thus, we will show that IR regulates miR-34a by increasing its expression...
January 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/27990356/microrna-34a-promoting-apoptosis-of-human-lens-epithelial-cells-through-down-regulation-of-b-cell-lymphoma-2-and-silent-information-regulator
#8
Qing-Lan Li, Hong-Yang Zhang, Yong-Jie Qin, Qian-Li Meng, Xiao-Lei Yao, Hai-Ke Guo
AIM: To investigate the role of microRNA-34a (miR-34a) in the induction of apoptosis of human lens epithelial (HLE-B3) cells. METHODS: The apoptosis of HLE-B3 cells was detected by Annexin V-PE apoptosis detection kit after the treatment with 200 µmol/L H2O2 for 24h and lentiviral miR-34a vector transfection. The expression of miR-34a in the cells was quantified by quantitative real time polymerase chain reaction (qRT-PCR) in response to H2O2 exposure and the vector transfection...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27981346/altered-expression-of-apoptosis-regulating-mirnas-in-salivary-gland-tumors-suggests-their-involvement-in-salivary-gland-tumorigenesis
#9
Bianca de Cássia Troncarelli de Campos Parra Flores, Silvia Vanessa Lourenço, Aline Santos Damascena, Luiz Paulo Kowaslki, Fernando Augusto Soares, Cláudia Malheiros Coutinho-Camillo
Salivary gland tumors comprise a heterogeneous group of lesions with different histological features and diverse clinical pathophysiology. They account for about 3% of all head and neck tumors. Apoptosis plays an important role during morphogenesis of glandular structures, including that of the salivary gland. Recent studies have demonstrated that several microRNAs (miRNAs) are involved in the control of apoptosis. The aim of the present study was to determine the expression of apoptosis-related miRNAs (miR-15a, miR-16, miR-17-5p, miR-20a, miR-21, miR-29, and miR-34) and their target mRNAs in 25 pleomorphic adenomas, 23 mucoepidermoid carcinomas, and 10 non-neoplastic salivary gland samples by real-time RT-PCR...
December 15, 2016: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/27979858/mir-34a-promotes-fibrosis-in-aged-lungs-by-inducing-alveolar-epithelial-dysfunctions
#10
Huachun Cui, Jing Ge, Na Xie, Sami Banerjee, Yong Zhou, Rui-Ming Liu, Victor J Thannickal, Gang Liu
Idiopathic pulmonary fibrosis (IPF) is a well-known age-related disease. However, much less recognized has been the aging associated pathogenesis of this disorder. As we and others previously showed that dysregulation of microRNAs (miRNAs) was an important mechanism involved in pulmonary fibrosis, the role of these molecules in this pathology in the aged population has not been investigated. In this study, by using lung fibrosis model established in old mice, we found that ablation of miR-34a protected aged animals from developing experimental lung fibrosis...
December 15, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27956809/disease-specific-mir-34a-as-diagnostic-marker-of-non-alcoholic-steatohepatitis-in-a-chinese-population
#11
Xiao-Lin Liu, Qin Pan, Rui-Nan Zhang, Feng Shen, Shi-Yan Yan, Chao Sun, Zheng-Jie Xu, Yuan-Wen Chen, Jian-Gao Fan
AIM: To assess disease-specific circulating microRNAs (miRNAs) in non-alcoholic steatohepatitis (NASH) patients. METHODS: A total of 111 biopsy-proven non-alcoholic fatty liver disease (NAFLD) or chronic hepatitis B (CHB) patients and healthy controls from mainland China were enrolled to measure their serum levels of miR-122, -125b, -146b, -16, -21, -192, -27b and -34a. The correlations between serum miRNAs and histological features of NAFLD were determined. The diagnostic value of miRNA in NASH and significant fibrosis was analyzed and compared with that of cytokeratin-18 (CK-18), fibrosis-4 (FIB-4), and aspartate aminotransferase to platelet ratio index (APRI), respectively...
November 28, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27939626/mir-34a-a-promising-novel-biomarker-for-benzene-toxicity-is-involved-in-cell-apoptosis-triggered-by-1-4-benzoquinone-through-targeting-bcl-2
#12
Yujiao Chen, Pengling Sun, Xiaoli Guo, Ai Gao
Exposure to benzene is inevitable, and concerns regarding the adverse health effects of benzene have been raised. Most investigators found that benzene exposure induced hematotoxicity. In this regard, Our study aimed to explore a novel potential biomarker of adverse health effects following benzene exposure and the toxic mechanisms of benzene metabolites in vitro. This study consisted of 314 benzene-exposed workers and 288 control workers, an air benzene concentration of who were 2.64 ± 1.60 mg/m(3) and 0...
February 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27924478/evaluating-synergistic-effects-of-mir-34a-mimics-in-combination-with-other-therapeutic-agents-in-cultured-non-small-cell-lung-cancer-cells
#13
Jane Zhao, Andreas G Bader
Tumor suppressor miRNAs such as miR-34a inhibit tumor growth by simultaneously regulating the expression of multiple important oncogenes across multiple oncogenic pathways and, therefore, provide a strong rationale for developing therapeutic miRNA mimics in combination with other therapeutic cancer agents to augment drug sensitivity. Here, we describe the experimental approach for evaluating miRNA and drug combinations using the "fixed ratio" method in cultured non-small cell lung cancer cells.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27917453/phase-i-study-of-mrx34-a-liposomal-mir-34a-mimic-administered-twice-weekly-in-patients-with-advanced-solid-tumors
#14
Muhammad S Beg, Andrew J Brenner, Jasgit Sachdev, Mitesh Borad, Yoon-Koo Kang, Jay Stoudemire, Susan Smith, Andreas G Bader, Sinil Kim, David S Hong
Purpose Naturally occurring tumor suppressor microRNA-34a (miR-34a) downregulates the expression of >30 oncogenes across multiple oncogenic pathways, as well as genes involved in tumor immune evasion, but is lost or under-expressed in many malignancies. This first-in-human, phase I study assessed the maximum tolerated dose (MTD), safety, pharmacokinetics, and clinical activity of MRX34, a liposomal miR-34a mimic, in patients with advanced solid tumors. Patients and Methods Adult patients with solid tumors refractory to standard treatment were enrolled in a standard 3 + 3 dose escalation trial...
December 5, 2016: Investigational New Drugs
https://www.readbyqxmd.com/read/27911398/evaluation-of-bioenergetic-function-in-cerebral-vascular-endothelial-cells
#15
Stephanie L Rellick, Heng Hu, James W Simpkins, Xuefang Ren
The integrity of the blood-brain-barrier (BBB) is critical to prevent brain injury. Cerebral vascular endothelial (CVE) cells are one of the cell types that comprise the BBB; these cells have a very high-energy demand, which requires optimal mitochondrial function. In the case of disease or injury, the mitochondrial function in these cells can be altered, resulting in disease or the opening of the BBB. In this manuscript, we introduce a method to measure mitochondrial function in CVE cells by using whole, intact cells and a bioanalyzer...
November 19, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27893432/microrna-34a-expression-levels-in-serum-and-intratumoral-tissue-can-predict-bone-metastasis-in-patients-with-hepatocellular-carcinoma
#16
Zuo-Lin Xiang, Xiao-Mei Zhao, Li Zhang, Ping Yang, Jia Fan, Zhao-You Tang, Zhao-Chong Zeng
Hepatocellular carcinoma (HCC) patients with bone metastasis (BM) suffer from pain and other symptoms that significantly reduce their quality of life. We screened a microRNA (miRNA) microarray to identify potential serum biomarkers for BM in HCC patients. A miRNA microarray was used to screen for BM-related miRNAs in paired serum samples from HCC patients with BM and from HCC patients without BM. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to quantify candidate miRNAs in serum samples from 106 independent HCC patients...
November 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/27889881/identification-of-reference-genes-and-mirnas-for-qrt-pcr-in-human-esophageal-squamous-cell-carcinoma
#17
Li Chen, Yulin Jin, Lin Wang, Fenghao Sun, Xiaodong Yang, Mengkun Shi, Cheng Zhan, Yu Shi, Qun Wang
It is important to select an appropriate reference gene and miRNA when using quantitative real-time polymerase chain reaction (qRT-PCR) to analyze gene and miRNA expression. However, many commonly used reference genes and miRNAs are not stably expressed and therefore not suitable for normalization or quantification of qRT-PCR data. This study aims to identify appropriate reference genes and miRNAs for use in human esophageal squamous carcinoma qRT-PCR analysis. Using data provided by The Cancer Genome Atlas, we identified DDX5, LAPTM4A, P4HB, RHOA, miR-28-5p, miR-34a-5p, and miR-186-5p as candidate reference genes and miRNAs...
January 2017: Medical Oncology
https://www.readbyqxmd.com/read/27889708/mir-34a-is-a-common-link-in-both-hiv-and-antiretroviral-therapy-induced-vascular-aging
#18
Jiaxin Zhan, Shanshan Qin, Lili Lu, Xiamin Hu, Jun Zhou, Yeying Sun, Jian Yang, Ying Liu, Zunzhe Wang, Ning Tan, Jiyan Chen, Chunxiang Zhang
Both HIV and antiretroviral therapy could induce vascular aging with unclear mechanisms. In this study, via microarray analysis, we identified, for the first time, that miR-34a expression was significantly increased in both HIV-infected, and antiretroviral agents-treated vessels and vascular endothelial cells (ECs) from these vessels. In cultured ECs, miR-34a expression was significantly increased by HIV-Tat protein and by the antiretroviral agents, lopinavir/ritonavir. Both HIV-Tat protein and antiretroviral agents could induce EC senescence, which was inhibited by miR-34a inhibition...
November 26, 2016: Aging
https://www.readbyqxmd.com/read/27885275/tdb-protects-vascular-endothelial-cells-against-oxygen-glucose-deprivation-reperfusion-induced-injury-by-targeting-mir-34a-to-increase-bcl-2-expression
#19
Li-Xi Liao, Ming-Bo Zhao, Xin Dong, Yong Jiang, Ke-Wu Zeng, Peng-Fei Tu
Prolonged ischemia can result in apoptotic death of vascular endothelial cells and lead to ischemic vascular diseases including vascular dementia, arteriosclerosis and brain oedema. Finding protective strategies to prevent this is therefore an urgent mission. Recent studies have shown that dysregulation of microRNAs (miRNAs) can lead to imbalance of Bcl-2 family proteins and mitochondrial dysfunction, leading to further damage of vascular cells under ischemic conditions. However, whether miRNAs can be used as a drug target for treating vascular diseases is not fully understood...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27879964/decreased-levels-of-mir-34a-and-mir-217-act-as-predictor-biomarkers-of-aggressive-progression-and-poor-prognosis-in-hepatocellular-carcinoma
#20
W Yu, Quan Shen, Qing F Jiang, Yao X Wang, Ke Li, Huan Z Xue
BACKGROUND: MicroRNAs (miRNAs) play key roles in tumor development and progression. The aim of this study was to explore the expression levels of miR-34a and miR-217 in hepatocellular carcinoma (HCC) and to further investigate the clinicopathological and prognostic value of miR-34a and miR-217. METHODS: The expression levels of miR-34a and miR-217 were evaluated using quantitative real-time PCR (qRT-PCR). Associations between these miRNAs expression and clinicopathological features were analyzed...
November 23, 2016: Minerva Medica
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