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https://www.readbyqxmd.com/read/29679131/process-optimization-involving-critical-evaluation-of-oxygen-transfer-oxygen-uptake-and-nitrogen-limitation-for-enhanced-biomass-and-lipid-production-by-oleaginous-yeast-for-biofuel-application
#1
Jayita Chopra, Ramkrishna Sen
Lipid accumulation in oleaginous yeast is generally induced by nitrogen starvation, while oxygen saturation can influence biomass growth. Systematic shake flask studies that help in identifying the right nitrogen source and relate its uptake kinetics to lipid biosynthesis under varying oxygen saturation conditions are very essential for addressing the bioprocessing-related issues, which are envisaged to occur in the fermenter scale production. In the present study, lipid bioaccumulation by P. guilliermondii at varying C:N ratios and oxygen transfer conditions (assessed in terms of kL a) was investigated in shake flasks using a pre-optimized N-source and a two-stage inoculum formulated in a hybrid medium...
April 20, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29678192/engineering-in-vitro-stem-cell-based-vascularized-bone-models-for-drug-screening-and-predictive-toxicology
#2
REVIEW
Alessandro Pirosa, Riccardo Gottardi, Peter G Alexander, Rocky S Tuan
The production of veritable in-vitro models of bone tissue is essential to understand the biology of bone and its surrounding environment, to analyze the pathogenesis of bone diseases (e.g., osteoporosis, osteoarthritis, osteomyelitis, etc.), to develop effective therapeutic drug screening, and to test potential therapeutic strategies. Dysregulated interactions between vasculature and bone cells are often related to the aforementioned pathologies, underscoring the need for a bone model that contains engineered vasculature...
April 20, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29677650/pollutants-degradation-performance-and-microbial-community-structure-of-aerobic-granular-sludge-systems-using-inoculums-adapted-at-mild-and-low-temperature
#3
Barbara Muñoz-Palazon, Chiara Pesciaroli, Alejandro Rodriguez-Sanchez, Jesús Gonzalez-Lopez, Alejandro Gonzalez-Martinez
Three aerobic granular sequencing batch reactors were inoculated using different inocula from Finland, Spain and a mix of both in order to investigate the effect over the degradation performance and the microbial community structure. The Finnish inoculum achieved a faster granulation and a higher depollution performance within the first two month of operation. However, after 90 days of operation, similar physico-chemical values were observed. On the other hand, the Real-time PCR showed that Archaea diminished from inoculum to granular biomass, while Bacteria and Fungi numbers remained stable...
April 13, 2018: Chemosphere
https://www.readbyqxmd.com/read/29676764/distribution-of-human-fecal-marker-gb-124-bacteriophages-in-urban-sewage-and-reclaimed-water-of-s%C3%A3-o-paulo-city-brazil
#4
Tatiana Prado, Antônio de Castro Bruni, Mikaela Renata Funada Barbosa, Vilma Marques Santos Bonanno, Suzi Cristina Garcia, Maria Inês Zanoli Sato
Bacteriophages infecting Bacteroides fragilis GB-124 have been described as potential markers of human fecal contamination in water sources. The aim of this study was to evaluate the occurrence of GB-124 phages in raw sewage, secondary effluents and reclaimed water of the São Paulo city using a low-cost microbial source tracking method. Samples were collected monthly from April 2015 to March 2016 in four municipal wastewater treatment plants that operate with activated sludge processes followed by different tertiary treatments (sand-anthracite filtration, membrane bioreactor/reverse osmosis) and final chlorination...
April 2018: Journal of Water and Health
https://www.readbyqxmd.com/read/29676747/membrane-integrated-process-for-advanced-treatment-of-high-strength-opium-alkaloid-wastewaters
#5
Güçlü Insel, Ahmet Karagunduz, Murat Aksel, Emine Cokgor, Gokce Kor-Bicakci, Goksin Ozyildiz, Ismail Toroz, Bulent Keskinler
In this study, an integrated aerobic membrane bioreactor (MBR)-nanofiltration (NF) system has been applied for advanced treatment of Opium processing wastewaters to comply with strict discharge limits. Aerobic MBR treatment was successfully applied to high strength industrial wastewater. In aerobic MBR treatment, a non-fouling unique slot aeration system was designed using computational fluid dynamics techniques. The MBR was used to separate treated effluent from dispersed and non-settleable biomass. Respirometric modeling using MBR sludge indicated that the biomass exhibited similar kinetic parameters to that of municipal activated sludge systems...
April 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29675803/drosophila-s2-cell-culture-in-a-wave-bioreactor-potential-for-scaling-up-the-production-of-the-recombinant-rabies-virus-glycoprotein
#6
Monize Caiado Decarli, Diogo Peres Dos Santos, Renato Mancini Astray, Daniella Cristina Ventini-Monteiro, Soraia Attie Calil Jorge, Daniela Matilde Correia, Juliana de Sá da Silva, Mayra Pereira Rocca, Hélio Langoni, Benedito Donizete Menozzi, Carlos Augusto Pereira, Claudio Alberto Torres Suazo
The transmembrane rabies virus glycoprotein (RVGP) is the main antigen of vaccine formulations used around the world to prevent rabies, the most lethal preventable infectious disease known. The objective of this work was to evaluate the potential of a bioreactor using wave-induced agitation in the initial steps of scaling up the rRVGP production process by a Drosophila melanogaster S2 cell line to produce rRVGP in sufficient quantities for immunization and characterization studies. Taking advantage of some remarkable features recognized in Drosophila S2 cells for scaling the culture process, a robust recombinant lineage (S2MtRVGPH-His) engineered by our group for the expression of rRVGP using a copper-inducible promoter was used in the bioreactor cultures...
April 19, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29675615/a-modular-approach-for-high-flux-lactic-acid-production-from-methane-in-an-industrial-medium-using-engineered-methylomicrobium-buryatense-5gb1
#7
Shivani Garg, James M Clomburg, Ramon Gonzalez
Convergence of market drivers such as abundant availability of inexpensive natural gas and increasing awareness of its global warming effects have created new opportunities for the development of small-scale gas-to-liquid (GTL) conversion technologies that can efficiently utilize methane, the primary component of natural gas. Leveraging the unique ability of methanotrophs that use methane as carbon and energy source, biological GTL platforms can be envisioned that are readily deployable at remote petroleum drilling sites where large chemical GTL infrastructure is uneconomical to set-up...
April 19, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29674014/quantitative-proteomics-of-bombyx-mori-after-bmnpv-challenge
#8
Fuxiang Mao, Jihai Lei, Obeng Enoch, Ming Wei, Cui Zhao, Yanping Quan, Wei Yu
The domesticated silkworm is an ideal and economic insect model that plays crucial roles in sericulture and bioreactor. Bombyx mori nucleopolyhedrovirus (BmNPV) is not only an infectious pathogen to B. mori, but also an efficient vector expressing recombinant proteins. Although, the proteomics of silkworm and BmN cell membrane lipid raft towards BmNPV infection had been investigated, proteome results of BmN cells upon BmNPV challenge currently remain ambiguous. In order to explore the interaction between silkworm and BmNPV, we analyzed several pivotal processes of BmNPV infected BmN cell by quantitative mass spectrometry...
April 16, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29673996/production-of-r-r-2-3-butanediol-of-ultra-high-optical-purity-from-paenibacillus-polymyxa-zj-9-using-homologous-recombination
#9
Li Zhang, Can Cao, Ruifan Jiang, Hong Xu, Feng Xue, Weiwei Huang, Hao Ni, Jian Gao
The present study describes the use of metabolic engineering to achieve the production of R,R-2,3-butanediol (R,R-2,3-BD) of ultra-high optical purity (>99.99%). To this end, the diacetyl reductase (DAR) gene (dud A) of Paenibacillus polymyxa ZJ-9 was knocked out via homologous recombination between the genome and the previously constructed targeting vector pRN5101-L'C in a process based on homologous single-crossover. PCR verification confirmed the successful isolation of the dud A gene disruption mutant P...
April 11, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29673960/systematic-metabolic-engineering-of-methylomicrobium-alcaliphilum-20z-for-2-3-butanediol-production-from-methane
#10
Anh Duc Nguyen, In Yeub Hwang, Ok Kyung Lee, Donghyuk Kim, Marina G Kalyuzhnaya, Rina Mariyana, Susila Hadiyati, Min Sik Kim, Eun Yeol Lee
Methane is considered a next-generation feedstock, and methanotrophic cell-based biorefinery is attractive for production of a variety of high-value compounds from methane. In this work, we have metabolically engineered Methylomicrobium alcaliphilum 20Z for 2,3-butanediol (2,3-BDO) production from methane. The engineered strain 20Z/pBudK.p, harboring the 2,3-BDO synthesis gene cluster (budABC) from Klebsiella pneumoniae, accumulated 2,3-BDO in methane-fed shake flask cultures with a titer of 35.66mg/L. Expression of the most efficient gene cluster was optimized using selection of promoters, translation initiation rates (TIR), and the combination of 2,3-BDO synthesis genes from different sources...
April 16, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29673624/an-efficient-method-to-control-high-mannose-and-core-fucose-levels-in-glycosylated-antibody-production-using-deoxymannojirimycin
#11
Sagit Shalel Levanon, Orit Aharonovitz, Ayelet Maor-Shoshani, Gita Abraham, Dan Kenett, Yehoshua Aloni
Glycosylation on the Fc region of recombinant Immunoglobulin G (IgG) therapeutic antibodies is a critical protein quality attribute which may affect the efficacy and safety of the molecule. During the development of biosimilar therapeutics, adjustment of the glycosylation profile is required in order to match the reference innovator profile. Deoxymannojirimycin (DMJ), a known inhibitor of mannosidase, was used in this study to modulate the glycosylation pattern of antibodies. The effect of DMJ, at concentrations of 5 μM - 500 μM, on non-fucosylated glycoform levels was tested in the biosynthesis processes of two different IgG1 (IgG1 #A and IgG1 #B) using two Chinese hamster ovary (CHO) cell lines (CHO-DXB-11 and CHOK1SV, respectively) in Erlenmeyer flasks and in lab scale bioreactors...
April 16, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29671385/artificial-neural-network-modeling-for-organic-and-total-nitrogen-removal-of-aerobic-granulation-under-steady-state-condition
#12
H Gong, R Pishgar, J H Tay
Aerobic granulation is a recent technology with high level of complexity and sensitivity to environmental and operational conditions. Artificial neural networks (ANN), computational tools capable of describing complex nonlinear systems, are the best fit to simulate aerobic granular bioreactors. In this study, two feedforward backpropagation ANN models were developed to predict chemical oxygen demand (COD) (Model I) and total nitrogen (TN) removal efficiencies (Model II) of aerobic granulation technology under steady-state condition...
April 19, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29669940/generation-and-testing-of-clinical-grade-exosomes-for-pancreatic-cancer
#13
Mayela Mendt, Sushrut Kamerkar, Hikaru Sugimoto, Kathleen M McAndrews, Chia-Chin Wu, Mihai Gagea, Sujuan Yang, Elena V Rodriges Blanko, Qian Peng, Xiaoyan Ma, Joseph R Marszalek, Anirban Maitra, Cassian Yee, Katayoun Rezvani, Elizabeth Shpall, Valerie S LeBleu, Raghu Kalluri
Exosomes are extracellular vesicles produced by all cells with a remarkable ability to efficiently transfer genetic material, including exogenously loaded siRNA, to cancer cells. Here, we report on a bioreactor-based, large-scale production of clinical-grade exosomes employing good manufacturing practice (GMP) standards. A standard operating procedure was established to generate engineered exosomes with the ability to target oncogenic Kras (iExosomes). The clinical-grade GMP iExosomes were tested in multiple in vitro and in vivo studies to confirm suppression of oncogenic Kras and an increase in the survival of several mouse models with pancreatic cancer...
April 19, 2018: JCI Insight
https://www.readbyqxmd.com/read/29669530/small-cell-and-non-small-cell-lung-cancer-form-metastasis-on-cellular-4d-lung-model
#14
Dhruva K Mishra, Ross A Miller, Kristi A Pence, Min P Kim
BACKGROUND: Metastasis is the main cause of death for lung cancer patients. The ex vivo 4D acellular lung model has been shown to mimic this metastatic process. However, the main concern is the model's lack of cellular components of the tumor's microenvironment. In this study, we aim to determine if the intact lung microenvironment will still allow lung cancer metastasis to form. METHODS: We harvested a heart-lung block from a rat and placed it in a bioreactor after cannulating the pulmonary artery, trachea and tying the right main bronchus for 10-15 days without any tumor cells as a control group or with NSCLC (A549, H1299 or H460), SCLC (H69, H446 or SHP77) or breast cancer cell lines (MCF7 or MDAMB231) through the trachea...
April 18, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29665956/laccase-production-in-bioreactor-scale-under-saline-condition-by-the-marine-derived-basidiomycete-peniophora-sp-cbmai-1063
#15
Pedro H Mainardi, Valker A Feitosa, Livia B Brenelli de Paiva, Rafaella C Bonugli-Santos, Fabio M Squina, Adalberto Pessoa, Lara D Sette
Laccase production in saline conditions is still poorly studied. The aim of the present study was to investigate the production of laccase in two different types of bioreactors by the marine-derived basidiomycete Peniophora sp. CBMAI 1063. The highest laccase activity and productivity were obtained in the Stirred Tank (ST) bioreactor, while the highest biomass concentration in Air-lift (AL) bioreactor. The main laccase produced was purified by ion exchange and size exclusion chromatography and appeared to be monomeric with molecular weight of approximately 55 kDa...
May 2018: Fungal Biology
https://www.readbyqxmd.com/read/29663323/single-pass-diafiltration-integrated-into-a-fully-continuous-mab-purification-process
#16
Joanna Rucker-Pezzini, Lindsay Arnold, Kevin Hill-Byrne, Tom Sharp, Maksim Avazhanskiy, Chris Forespring
The concept of continuous manufacturing has gained significant interest from the biopharmaceutical industry over the past several years. Benefits include increased manufacturing productivity, improved quality control, reduction in plant footprint, and more flexible management of facility capacity. There are several technologies currently available that enable continuous processing for chromatography and ultrafiltration. However, a single pass diafiltration design that meets the required small molecule clearance and has been integrated into a fully continuous monoclonal antibody purification process has not been previously published...
April 16, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29662670/extended-bioreactor-conditioning-of-mononuclear-cell-seeded-heart-valve-scaffolds
#17
Mitchell VeDepo, Eric Buse, Rachael Quinn, Richard Hopkins, Gabriel Converse
The tissue-engineered heart valve may be the ideal valve replacement option but still must overcome challenges in leaflet recellularization. This study sought to investigate the potential for leaflet matrix restoration and repopulation following mononuclear cell seeding and extended periods of bioreactor conditioning. Human aortic heart valves were seeded with mononuclear cells and conditioned in a pulsatile bioreactor for 3 days, 3 weeks, or 6 weeks. The results of this study determined that a mononuclear cell population can be readily localized within the leaflet tissue in as little as 3 days...
January 2018: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29662147/a-methanotrophic-archaeon-couples-anaerobic-oxidation-of-methane-to-fe-iii-reduction
#18
Chen Cai, Andy O Leu, Guo-Jun Xie, Jianhua Guo, Yuexing Feng, Jian-Xin Zhao, Gene W Tyson, Zhiguo Yuan, Shihu Hu
Microbially mediated anaerobic oxidation of methane (AOM) is a key process in the regulation of methane emissions to the atmosphere. Iron can serve as an electron acceptor for AOM, and it has been suggested that Fe(III)-dependent AOM potentially comprises a major global methane sink. Although it has been proposed that anaerobic methanotrophic (ANME) archaea can facilitate this process, their active metabolic pathways have not been confirmed. Here we report the enrichment and characterisation of a novel archaeon in a laboratory-scale bioreactor fed with Fe(III) oxide (ferrihydrite) and methane...
April 16, 2018: ISME Journal
https://www.readbyqxmd.com/read/29661978/control-of-cell-behaviour-through-nanovibrational-stimulation-nanokicking
#19
REVIEW
Shaun N Robertson, Paul Campsie, Peter G Childs, Fiona Madsen, Hannah Donnelly, Fiona L Henriquez, William G Mackay, Manuel Salmerón-Sánchez, Monica P Tsimbouri, Craig Williams, Matthew J Dalby, Stuart Reid
Mechanical signals are ubiquitous in our everyday life and the process of converting these mechanical signals into a biological signalling response is known as mechanotransduction. Our understanding of mechanotransduction, and its contribution to vital cellular responses, is a rapidly expanding field of research involving complex processes that are still not clearly understood. The use of mechanical vibration as a stimulus of mechanotransduction, including variation of frequency and amplitude, allows an alternative method to control specific cell behaviour without chemical stimulation (e...
May 28, 2018: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/29661028/development-of-large-scale-manufacturing-of-adipose-derived-stromal-cells-for-clinical-applications-using-bioreactors-and-human-platelet-lysate
#20
Mandana Haack-Sørensen, Morten Juhl, Bjarke Follin, Rebekka Harary Søndergaard, Maria Kirchhoff, Jens Kastrup, Annette Ekblond
In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into a large-scale, allogeneic production process for clinical applications necessitates well-chosen raw materials and cell culture platform. We compare the use of clinical-grade human platelet lysate (hPL) and fetal bovine serum (FBS) as growth supplements for ASC expansion in the automated, closed hollow fibre quantum cell expansion system (bioreactor)...
April 17, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
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