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https://www.readbyqxmd.com/read/29241127/high-strength-n-methyl-2-pyrrolidone-containing-process-wastewater-treatment-using-sequencing-batch-reactor-and-membrane-bioreactor-a-feasibility-study
#1
Chun Heng Loh, Bing Wu, Liya Ge, Chaozhi Pan, Rong Wang
N-methyl-2-pyrrolidone (NMP) is widely used as a solvent in polymeric membrane fabrication process, its elimination from the process wastewater (normally at a high concentration > 1000 mg/L) prior to discharge is essential because of environmental concern. This study investigated the feasibility of treating high-strength NMP-containing process wastewater in a sequencing batch reactor (SBR; i.e., batch feeding and intermittent aerobic/anoxic condition) and a membrane bioreactor (MBR; i.e., continuous feeding and aeration), respectively...
December 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/29237993/-eukaryotic-microbiomes-of-membrane-attached-biofilms-in-membrane-bioreactors-analyzed-by-high-throughput-sequencing-and-microscopic-observations
#2
Tomohiro Inaba, Tomoyuki Hori, Yuya Sato, Tomo Aoyagi, Dai Hanajima, Atsushi Ogata, Hiroshi Habe
Limited information is currently available on the contribution of eukaryotes to the reactor performance of membrane bioreactors (MBRs). Using high-throughput Illumina sequencing of 18S rRNA genes and microscopic observations, we investigated eukaryotic microbiomes in membrane-attached biofilms in MBRs treating piggery wastewater. Protozoa preying on bacteria were frequently detected under stable conditions when membrane clogging was suppressed. However, the eukaryotes preying upon protozoa became predominant in biofilms when membrane fouling rapidly progressed...
December 12, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/29236012/organic-matter-degradation-in-a-greywater-recycling-system-using-a-multistage-moving-bed-biofilm-reactor-mbbr
#3
Assia Saidi, Khaoula Masmoudi, Erwin Nolde, Btissam El Amrani, Fouad Amraoui
Greywater is an important non-conventional water resource which can be treated and recycled in buildings. A decentralized greywater recycling system for 223 inhabitants started operating in 2006 in Berlin, Germany. High load greywater undergoes advanced treatment in a multistage moving bed biofilm reactor (MBBR) followed by sand filtration and UV disinfection. The treated water is used safely as service water for toilet flushing. Monitoring of the organic matter degradation was pursued to describe the degradation processes in each stage and optimize the system...
December 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29235931/impact-of-poly-dimethyldiallylammonium-chloride-on-membrane-fouling-mitigation-in-membrane-bioreactor
#4
Jia-Heng Zhou, Gao-Feng Cheng, Chang-Hua Wu, Qian-Kun Hong, Yao-Zhong Li, Hong-Yu Wang
Poly dimethyldiallylammonium chloride (PDMDAAC) was applied in membrane bioreactor (MBR) to study its effects on mitigation of MBR membrane fouling. Floc size, zeta potential, soluble microbial substances (SMP) and extracellular polymeric substances (EPS) secretion were studied with respect to PDMMAAC dosing operations. Results demonstrated that sustainable filtration cycle extended 3.3 times with the optimal PDMDAAC dosage of 90 mg L-1. The addition of PDMDAAC could increase zeta potential of sludge floc, which led to the decrease of repulsive electrostatic interactions between flocs, as well as the facilitation of flocs-to-flocs aggregation...
December 13, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29235926/effect-of-powdered-activated-carbon-dosage-on-sludge-properties-and-membrane-bioreactor-performance-in-a-hybrid-mbr-pac-system
#5
Shi Zhang, Xingtao Zuo, Juan Xiong, Cong Ma, Bo Hu
An improved insight into the effect of powdered activated carbon (PAC) on membrane fouling is crucial to the MBR performance. Sludge key property, soluble microbial products (SMP) and transmembrane pressure (TMP) were monitored. The membrane fouling rate in the MBRs was also analyzed based on TMP profile and resistance-in-series model. PAC reduced the membrane filtration resistance and significantly decreased the fouling rate. The sludge filterability was improved by extending the filtration time by almost two-fold...
December 13, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29235728/semicontinuous-sophorolipid-fermentation-using-a-novel-bioreactor-with-dual-ventilation-pipes-and-dual-sieve-plates-coupled-with-a-novel-separation-system
#6
Yaguang Zhang, Dan Jia, Wanqi Sun, Xue Yang, Chuanbo Zhang, Fanglong Zhao, Wenyu Lu
Sophorolipids (SLs) are biosurfactants with widespread applications. The yield and purity of SLs are two important factors to be considered during their commercial large-scale production. Notably, SL accumulation causes an increase in viscosity, decrease in dissolved oxygen and product inhibition in the fermentation medium. This inhibits the further production and purification of SLs. This describes the development of a novel integrated system for SL production using Candida albicans O-13-1. Semicontinuous fermentation was performed using a novel bioreactor with dual ventilation pipes and dual sieve-plates (DVDSB)...
December 13, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29235057/design-and-assessment-of-a-dynamic-perfusion-bioreactor-for-large-bone-tissue-engineering-scaffolds
#7
Birru Bhaskar, Robert Owen, Hossein Bahmaee, Parcha Sreenivasa Rao, Gwendolen C Reilly
Bioreactors can be used to apply fluid flow in vitro to scaffolds to improve mass transport of media and apply mechanical forces to cells. In this study, we developed and tested an autoclavable, modular perfusion bioreactor suitable for large scaffolds. We investigated the effects of fluid flow induced shear stress (FFSS) on osteogenic differentiation of human embryonic stem cell-derived mesenchymal progenitors (hES-MP cells) cultured on large polyurethane (PU) scaffolds (30 mm diameter × 5 mm thickness) in osteogenesis induction media (OIM)...
December 12, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29233554/accelerated-and-improved-differentiation-of-retinal-organoids-from-pluripotent-stem-cells-in-rotating-wall-vessel-bioreactors
#8
Tyler DiStefano, Holly Yu Chen, Christopher Panebianco, Koray Dogan Kaya, Matthew J Brooks, Linn Gieser, Nicole Y Morgan, Tom Pohida, Anand Swaroop
Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a polarized, laminated architecture. In static cultures, organoid development may be hindered by limitations in diffusion of oxygen and nutrients. Herein, we report a bioprocess using rotating-wall vessel (RWV) bioreactors to culture retinal organoids derived from mouse pluripotent stem cells. Organoids in RWV demonstrate enhanced proliferation, with well-defined morphology and improved differentiation of neurons including ganglion cells and S-cone photoreceptors...
December 1, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29233011/immobilized-inulinase-a-new-horizon-of-paramount-importance-driving-the-production-of-sweetener-and-prebiotics
#9
Gerard Neeraj, Shobana Ravi, Ravindran Somdutt, ShriAishvarya Kaliyur Ravi, Vaidyanathan Vinoth Kumar
In recent times, inulinase has emerged as one the most prominent and industrially upcoming enzymes applied to meet the ever increasing demand of d-fructose and fructooligosaccharides (FOS) as sweetener and prebiotics in the food and pharmaceutical industry, respectively. This review deals with types of inulinase and the attempts made to modify it for better thermal stability and shelf life. The ease of immobilization of inulinase has led us to the path of experimenting with different methods of enzyme immobilization since 1979...
December 12, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29232676/progressive-development-of-aberrant-smooth-muscle-cell-phenotype-in-abdominal-aortic-aneurysm-disease
#10
Kirsten Riches, Emily Clark, Rebecca J Helliwell, Timothy G Angelini, Karen E Hemmings, Marc A Bailey, Katherine I Bridge, D Julian A Scott, Karen E Porter
Abdominal aortic aneurysm (AAA) is a silent, progressive disease with a high mortality and an increasing prevalence with aging. Smooth muscle cell (SMC) dysfunction contributes to gradual dilatation and eventual rupture of the aorta. Here we studied phenotypic characteristics in SMC cultured from end-stage human AAA (≥5 cm) and cells cultured from a porcine carotid artery (PCA) model of early and end-stage aneurysm. Human AAA-SMC presented a secretory phenotype and expressed elevated levels of the differentiation marker miR-145 (2...
December 13, 2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/29230528/cofactor-f430-as-a-biomarker-for-methanogenic-activity-application-to-an-anaerobic-bioreactor-system
#11
I Passaris, P Van Gaelen, R Cornelissen, K Simoens, D Grauwels, L Vanhaecke, D Springael, I Smets
Over the last decades, anaerobic bioreactor technology proved to be a competitive technology for purifying wastewater while producing biogas. Methanogens perform the crucial final step in methane production, and monitoring their activity is of paramount importance for system understanding and management. Cofactor F430 is an essential prosthetic group of the methyl-coenzyme M reductase (MCR) enzyme catalysing this final step. This research investigates whether the quantification of cofactor F430 in bioreactor systems is a viable intermediate-complexity monitoring tool in comparison to the conventional biogas and volatile fatty acid (VFA) concentration follow-up and molecular genetic techniques targeting the mcrA gene encoding the MCR protein or its transcripts...
December 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29230389/cyclic-tensile-strain-can-play-a-role-in-directing-both-intramembranous-and-endochondral-ossification-of-mesenchymal-stem-cells
#12
Simon F Carroll, Conor T Buckley, Daniel J Kelly
Successfully regenerating damaged or diseased bone and other joint tissues will require a detailed understanding of how joint specific environmental cues regulate the fate of progenitor cells that are recruited or delivered to the site of injury. The goal of this study was to explore the role of cyclic tensile strain (CTS) in regulating the initiation of mesenchymal stem cell/multipotent stromal cell (MSC) differentiation, and specifically their progression along the endochondral pathway. To this end, we first explored the influence of CTS on the differentiation of MSCs in the absence of any specific growth factor, and secondly, we examined the influence of the long-term application of this mechanical stimulus on markers of endochondral ossification in MSCs maintained in chondrogenic culture conditions...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29230151/integrating-a-19f-mri-tracer-agent-into-the-clinical-scale-manufacturing-of-a-t-cell-immunotherapy
#13
Charles F O'Hanlon, Tamara Fedczyna, Shannon Eaker, William D Shingleton, Brooke M Helfer
Leukocyte immunotherapies have made great progress in the treatment of cancer. Recent reports on the treatment of B-cell malignancies using Chimeric Antigen Receptor and affinity enhanced T-Cell Receptor therapies have demonstrated encouraging clinical results. As investigators begin to explore the treatment of solid tumors with these cells, the hurdle of evaluating T-cell homing to and persistence at the site of disease remain. Significant challenges regarding the GMP manufacture and administration of a therapeutic dose of millions to billions of transduced T-cells remain...
2017: Contrast Media & Molecular Imaging
https://www.readbyqxmd.com/read/29228335/gram-scale-production-of-recombinant-microbial-enzymes-in-shake-flasks
#14
Lukas Chrast, Radka Chaloupkova, Jiri Damborsky
Heterologous production of recombinant proteins is a cornerstone of microbiological and biochemical research as well as various biotechnological processes. Yields and quality of produced proteins have a tremendous impact on structural and enzymology studies, development of new biopharmaceuticals and establishing new biocatalytic processes. Majority of current protocols for recombinant protein expression in Escherichia coli exploit batch cultures with complex media, often providing low yields of the target protein due to oxygen transfer limitation, rapid depletion of carbon sources, and pH changes during the cultivation...
December 5, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29227871/production-of-biogenic-manganese-oxides-coupled-with-methane-oxidation-in-a-bioreactor-for-removing-metals-from-wastewater
#15
Shuji Matsushita, Daisuke Komizo, Linh Thi Thuy Cao, Yoshiteru Aoi, Tomonori Kindaichi, Noriatsu Ozaki, Hiroyuki Imachi, Akiyoshi Ohashi
Biogenic manganese oxide (BioMnOx) can efficiently adsorb various minor metals. The production of BioMnOx in reactors to remove metals during wastewater treatment processes is a promising biotechnological method. However, it is difficult to preferentially enrich manganese-oxidizing bacteria (MnOB) to produce BioMnOx during wastewater treatment processes. A unique method of cultivating MnOB using methane-oxidizing bacteria (MOB) to produce soluble microbial products is proposed here. MnOB were successfully enriched in a methane-fed reactor containing MOB...
December 1, 2017: Water Research
https://www.readbyqxmd.com/read/29226613/large-scale-culture-of-a-megakaryocytic-progenitor-cell-line-with-a-single-use-bioreactor-system
#16
Retno Wahyu Nurhayati, Yoshihiro Ojima, Takeaki Dohda, Masahiro Kino-Oka
The increasing application of regenerative medicine has generated a growing demand for stem cells and their derivatives. Single-use bioreactors offer an attractive platform for stem cell expansion owing to their scalability for large-scale production and feasibility of meeting clinical-grade standards. The current work evaluated the capacity of a single-use bioreactor system (1 L working volume) for expanding Meg01 cells, a megakaryocytic (MK) progenitor cell line. Oxygen supply was provided by surface aeration to minimize foaming and orbital shaking was used to promote oxygen transfer...
December 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29226358/mechanical-evaluation-of-tracheal-grafts-on-different-scales
#17
REVIEW
Fabio Gava Aoki, Henrique Takachi Moriya
Tissue engineered (or bioengineered) tracheas are alternative options under investigation when the resection with end-to-end anastomosis cannot be performed. One approach to develop bioengineered tracheas is a complex process that involves the use of decellularized tissue scaffolds, followed by recellularization in custom-made tracheal bioreactors. Tracheas withstand pressure variations and their biomechanics are of great importance so that they do not collapse during respiration, although there has been no preferred method of mechanical assay of tracheas among several laboratories over the years...
December 11, 2017: Artificial Organs
https://www.readbyqxmd.com/read/29226181/a-bioreactor-method-to-generate-high-titer-genetically-stable-clinical-isolate-human-cytomegalovirus
#18
Victoria R Saykally, Luke I Rast, Jeff Sasaki, Seung-Yong Jung, Cynthia Bolovan-Fritts, Leor S Weinberger
Human cytomegalovirus (HCMV) infection is a major cause of morbidity and mortality in transplant patients and a leading cause of congenital birth defects (Saint Louis, 2016). Vaccination and therapeutic studies often require scalable cell culture production of wild type virus, represented by clinical isolates. Obtaining sufficient stocks of wild-type clinical HCMV is often labor intensive and inefficient due to low yield and genetic loss, presenting a barrier to studies of clinical isolates. Here we report a bioreactor method based on continuous infection, where retinal pigment epithelial (ARPE-19) cells adhered to microcarrier beads are infected in a bioreactor and used to produce high-titers of clinical isolate HCMV that maintain genetic integrity of key viral tropism factors and the viral genome...
November 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/29224272/-the-development-and-status-of-bioartificial-liver
#19
G L Li, S M Wang
Liver failure is a serious stage during liver disease development of which mobidity is high. There is no effective treatment at present.Artificial liver support system is one of the important methods to treat liver failure which includes non-biological artificial liver, biological artificial liver and hybrid artificial liver. Among the artificial liver devices. The bioartificial liver is the most similar artifical liver device to human liver in terms of detoxification, synthesis and metabolism currently.The complexity of human liver function makes the biological artificial liver facing great challenges in selection of liver seed cells, construction of bioreactor and the best combination with auxiliary device, which leads to the slow development of bioartificial liver...
December 1, 2017: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/29224081/electrochemical-applications-in-metal-bioleaching
#20
Christoph Kurt Tanne, Axel Schippers
Biohydrometallurgy comprises the recovery of metals by biologically catalyzed metal dissolution from solids in an aqueous solution. The application of this kind of bioprocessing is described as "biomining," referring to either bioleaching or biooxidation of sulfide metal ores. Acidophilic iron- and sulfur-oxidizing microorganisms are the key to successful biomining. However, minerals such as primary copper sulfides are recalcitrant to dissolution, which is probably due to their semiconductivity or passivation effects, resulting in low reaction rates...
December 10, 2017: Advances in Biochemical Engineering/biotechnology
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