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Kuo-Hwa Wang, Richard Wan, Li-Hsuan Chiu, Yu-Hui Tsai, Chia-Lang Fang, John F Bowley, Kuan-Chou Chen, Hsin-Nung Shih, Wen-Fu Thomas Lai
[This corrects the article DOI: 10.1371/journal.pone.0196779.].
2018: PloS One
N Lopez-Ramirez, T Volke-Sepulveda, I Gaime-Perraud, G Saucedo-Castañeda, E Favela-Torres
A novel bench-scale stirred bioreactor for solid-state fermentation was used to determine the effect of the stirring rate on growth and enzymes production by Trichoderma harzianum PBLA. Lab-scale static tubular bioreactors were first used to assess the effect of bioreactor diameter on heat accumulation, growth, and production of cellulases and xylanases. The increased diameters (1.8-4.2 cm) led to increases in temperature up to 36 °C (at a rate of 1.08 °C/cm), which negatively affected the growth and enzyme production...
June 14, 2018: Bioresource Technology
Nabin Aryal, Torben Kvist, Fariza Ammam, Deepak Pant, Lars D M Ottosen
Biogas upgrading technologies have received widespread attention recently and are researched extensively. Microbial biogas upgrading (biomethanation) relies on the microbial performance in enriched H2 and CO2 environments. In this review, recent developments and applications of CH4 enrichment in microbial methanation processes are systematically reviewed. During biological methanation, either H2 can be injected directly inside the anaerobic digester to enrich CH4 by a consortium of mixed microbial species or H2 can be injected into a separate bioreactor, where CO2 contained in biogas is coupled with H2 and converted to CH4 , or a combination hereof...
June 14, 2018: Bioresource Technology
Abdallah R Ismail, Samy B El-Henawy, Sherif A Younis, Mohamed A Betiha, Nour Sh El-Gendy, Mohamed S Azab, Nagwa M Sedky
THE AIM: of this study was to isolate and identify lipolytic bacteria. Perform a statistical stepwise physicochemical optimization for maximum production of extracellular lipase and its validation in a bioreactor. METHODS AND RESULTS: Several lipolytic bacteria were isolated from petroleum hydrocarbon polluted soil. The strain expressing the highest lipase activity (47 U ml-1 ) was genetically identified as Gram +ve Bacillus stratosphericus PSP8 (NCBI GenBank Accession no...
June 16, 2018: Journal of Applied Microbiology
Qianwen Sui, Chao Jiang, Junya Zhang, Dawei Yu, Meixue Chen, Yawei Wang, Yuansong Wei
Swine wastes are the reservoir of antibiotic resistance genes (ARGs), which can potentially spread from swine farms to the environment. This study establishes a sequencing-batch membrane bioreactor (SMBR) for ARG removal from swine wastewater, and analyzes the effect of biological treatment and membrane separation on the ARG removal at different solid retention times (SRTs). The SMBR removed 2.91 logs (copy number) of ARGs at a short SRT (12 days). Raising the SRT reduced the removal rates of the detected genes by the biological treatment...
June 12, 2018: Environment International
Martina M De Santis, Deniz A Bölükbas, Sandra Lindstedt, Darcy E Wagner
Chronic respiratory diseases remain a major cause of morbidity and mortality worldwide. The only option at end-stage disease is lung transplantation, but there are not enough donor lungs to meet clinical demand. Alternative options to increase tissue availability for lung transplantation are urgently required to close the gap on this unmet clinical need. A growing number of tissue engineering approaches are exploring the potential to generate lung tissue ex vivo for transplantation. Both biologically derived and manufactured scaffolds seeded with cells and grown ex vivo have been explored in pre-clinical studies, with the eventual goal of generating functional pulmonary tissue for transplantation...
June 14, 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Yani Dai, Xuezhen Yang, Shen Liu, Jianwu Wang, Qixian Zhou, Rui Fang, Tian Yu
OBJECTIVE: To study the edible safety of recombinant human lactoferrin( rh LF) expressed from transgenic cow mammary gland bioreactor. METHODS: According to the food additive safety toxicology evaluation procedures and method, acute oral toxicity in rats and 90 day subchronic toxicity test in mice were done to evaluate the edible safety of rh LF. RESULTS: Acute oral toxicity test indicated that rh LF was no toxic effect during the observation period, mouse acute oral LD50 of recombinant human lactoferrin was greater than 20 000 mg/kg...
March 2018: Wei Sheng Yan Jiu, Journal of Hygiene Research
Yongjun Xia, Yan Chen, Xiaofeng Liu, Xuan Zhou, Zhaochu Wang, Guangqiang Wang, Zhiqiang Xiong, Lianzhong Ai
BACKGROUND: Antroquinonol, a ubiquinone derivative that shows anticancer and anti-inflammatory activities, is produced during solid-state fermentation of A. camphorata, however, it cannot be biosynthesized via conventional submerged fermentation. RESULTS: A method for enhancing the biosynthesis of Antroquinonol by controlling pH and adding oxygen vector in 7L bioreactor was studied. In shake flask experiments, a maximum Antroquinonol production of 31.39 ± 0.78 mg/L was obtained by fermentation with adding 0...
June 14, 2018: Journal of the Science of Food and Agriculture
Qiuzhen Wang, Huike Ye, Biswarup Sen, Yunxuan Xie, Yaodong He, Sunghoon Park, Guangyi Wang
Thraustochytrids, rich in docosahexaenoic acid (DHA, C22:6ω3), represent a potential source of dietary fatty acids. Yet, the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains. Two different thraustochytrid strains, Schizochytrium sp. PKU#Mn4 and Thraustochytriidae sp. PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions. Although they exhibited similar fatty acid profiles, PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions...
June 2018: Synthetic and Systems Biotechnology
Daniel Bello-Gil, Emma Roig-Molina, Jennifer Fonseca, María Dolores Sarmiento-Ferrández, Marcela Ferrándiz, Esther Franco, Elena Mira, Beatriz Maestro, Jesús M Sanz
The presence of synthetic dyes in wastewaters generated by the textile industry constitutes a serious environmental and health problem that urges the scientific community on an appropriate action. As a proof-of-concept, we have developed a novel approach to design enzymatic bioreactors with the ability to decolorize dye solutions through the immobilization of the bacterial CueO laccase-like multicopper oxidase from Escherichia coli on polyhydroxybutyrate (PHB) beads by making use of the BioF affinity tag. The decolorization efficiency of the system was characterized by a series of parameters, namely maximum enzyme adsorption capacity, pH profile, kinetic constants, substrate range, temperature and bioreactor recycling...
June 12, 2018: Microbial Biotechnology
Patrick Ovando-Roche, Emma L West, Matthew J Branch, Robert D Sampson, Milan Fernando, Peter Munro, Anastasios Georgiadis, Matteo Rizzi, Magdalena Kloc, Arifa Naeem, Joana Ribeiro, Alexander J Smith, Anai Gonzalez-Cordero, Robin R Ali
BACKGROUND: The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies and disease modelling relies on the ability to obtain healthy and organised retinal tissue in sufficient quantities. Generating such tissue is a lengthy process, often taking over 6 months of cell culture, and current approaches do not always generate large quantities of the major retinal cell types required. METHODS: We adapted our previously described differentiation protocol to investigate the use of stirred-tank bioreactors...
June 13, 2018: Stem Cell Research & Therapy
Olusegun K Abass, Fang Fang, Maoshui Zhuo, Kaisong Zhang
Studies on membrane fouling during treatment of oil refinery wastewater (ORW) via membrane bioreactor (MBR) are currently lacking, and associated fouling challenges are largely undocumented. Using advanced chemical and Illumina sequencing approach, we investigated the complex bio-physiochemical interactions responsible for foulant-membrane interactions during treatment of ORW. After nearly 2 months of the MBR operation, COD removal reached maximal of 97.15 ± 1.85%, while oil and grease removal was maintained at 96...
June 9, 2018: Science of the Total Environment
Xuemeng He, Lili Ding, Wenbo Su, Haijun Ma, Hui Huang, Yanru Wang, Hongqiang Ren
Endotoxins are potential toxics impacting human health through respiration derived in wastewater treatment plants (WWTPs), yet the formation of endotoxins during wastewater treatment processes is still lacking research. In our study, the distribution of endotoxins and bacterial community structure in the wastewater of three full scale pharmaceutical WWTPs were explored using the limulus amebocyte lysate (LAL) test and MiSeq technique. Results showed that higher endotoxin activities in the influent of Plant A and Plant C (560 and 1140 EU/mL), stemming from the fermentation process, were found compared to that of Plant B (135 EU/mL), coming from the process of chemical synthesis...
June 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Youhong Lin, Xiaying Cheng, Dongfeng Yang, Zongsuo Liang, Zongqi Yang
In recent years, gene engineering is developing rapidly and many recombinant proteins have been expressed. The use of plant bioreactor to express specific pharmaceutical proteins provides a new way for the prevention and treatment of some important diseases in human beings. Nowadays, chloroplast genetic transformation and expression system has become a research hotspot in plant bioreactor. Higher plant chloroplasts have unique advantages in the expression of recombinant proteins due to their special structures and inherited characteristics: such as high expression, site-specific integration, and the maternal inheritance characteristics of exogenous genes...
May 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Szymon Skoneczny
The paper presents a mathematical model and steady-state characteristics of a continuous-flow two-phase fluidized-bed bioreactor with external aeration. The quantitative description includes biofilm growth on inert carriers, distributions of density and diffusion coefficients in biofilm, decay of microorganisms, gas-liquid and liquid-biofilm mass transfer, biofilm detachment, biomass thickening and recirculation. The method for the determination of steady-states and the assessment of stability based on the orthogonal collocation method was presented...
June 12, 2018: Biotechnology Progress
Chang Liu, Lin Chen, Liang Zhu
This study was carried out by applying the direct contact membrane distillation (DCMD) into the treatment of effluent from anaerobic membrane bioreactor. The treatment efficiency of DCMD was highly emphasized, which was expected to be improved through the optimization of operating conditions. Three operating conditions, including temperature difference, cross-flow velocity and membrane pore size, were considered. The relative flux (the ratio of actual flux to initial flux) increased from 0.50 to 0.98 as the operating conditions changed and that was enhanced by the increment of temperature difference and cross-flow velocity...
June 4, 2018: Chemosphere
Jennifer E Schollée, Marc Bourgin, Urs von Gunten, Christa S McArdell, Juliane Hollender
Ozonation and subsequent post-treatments are increasingly implemented in wastewater treatment plants (WWTPs) for enhanced micropollutant abatement. While this technology is effective, micropollutant oxidation leads to the formation of ozonation transformation products (OTPs). Target and suspect screening provide information about known parent compounds and known OTPs, but for a more comprehensive picture, non-target screening is needed. Here, sampling was conducted at a full-scale WWTP to investigate OTP formation at four ozone doses (2, 3, 4, and 5 mg/L, ranging from 0...
May 25, 2018: Water Research
Nadia Rbia, Liselotte F Bulstra, Allen T Bishop, Andre J van Wijnen, Alexander Y Shin
BACKGROUND: The addition of adipose-derived Mesenchymal Stromal Cells (MSCs) to decellularized nerve allografts may improve outcomes of nerve reconstruction. Prior techniques used for cell seeding are traumatic to both the MSCs and nerve graft. An adequate, reliable and validated cell seeding technique is an essential step for evaluating the translational utility of MSC-enhanced decellularized nerve grafts. The purpose of this study was to develop a simple seeding strategy with an optimal seeding duration...
May 22, 2018: Plastic and Reconstructive Surgery
Lan Anh Thi Hoang, Ha Cam Nguyen, Thom Thi Le, Thi Huong Quynh Hoang, Van Nhat Pham, Minh Hien Thi Hoang, Hoai Thu Thi Ngo, Dang Diem Hong
Schizochytrium mangrovei strain PQ6 was investigated for co-production of docosahexaenoic acid (C22: 6ω-3, DHA) and squalene using a 30 L bioreactor with a working volume of 15 L under various batch and fed-batch fermentation process regimes. The fed-batch process was a more efficient cultivation strategy for achieving higher biomass production rich in DHA and squalene. The final biomass, total lipid, unsaponifiable lipid content and DHA productivity were 105.25 g · L-1 , 43.40% of dry cell weight, 8.58% total lipid and 61...
June 11, 2018: Journal of Phycology
Brandon G Wong, Christopher P Mancuso, Szilvia Kiriakov, Caleb J Bashor, Ahmad S Khalil
Precise control over microbial cell growth conditions could enable detection of minute phenotypic changes, which would improve our understanding of how genotypes are shaped by adaptive selection. Although automated cell-culture systems such as bioreactors offer strict control over liquid culture conditions, they often do not scale to high-throughput or require cumbersome redesign to alter growth conditions. We report the design and validation of eVOLVER, a scalable do-it-yourself (DIY) framework, which can be configured to carry out high-throughput growth experiments in molecular evolution, systems biology, and microbiology...
June 11, 2018: Nature Biotechnology
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