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https://www.readbyqxmd.com/read/28215046/genome-resolved-metagenomics-of-a-bioremediation-system-for-degradation-of-thiocyanate-in-mine-water-containing-suspended-solid-tailings
#1
Sumayah F Rahman, Rose S Kantor, Robert Huddy, Brian C Thomas, Andries W van Zyl, Susan T L Harrison, Jillian F Banfield
Thiocyanate (SCN(-) ) is a toxic compound that forms when cyanide (CN(-) ), used to recover gold, reacts with sulfur species. SCN(-) -degrading microbial communities have been studied, using bioreactors fed synthetic wastewater. The inclusion of suspended solids in the form of mineral tailings, during the development of the acclimatized microbial consortium, led to the selection of an active planktonic microbial community. Preliminary analysis of the community composition revealed reduced microbial diversity relative to the laboratory-based reactors operated without suspended solids...
February 19, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28209146/heterologous-expression-of-aspergillus-aculeatus-endo-polygalacturonase-in-pichia-pastoris-by-high-cell-density-fermentation-and-its-application-in-textile-scouring
#2
Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua, Duriya Chantasingh
BACKGROUND: Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. RESULTS: The recombinant EndoPG exhibited a specific activity of 1892...
February 16, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28203403/clinic-application-of-tissue-engineered-bronchus-for-lung-cancer-treatment
#3
Qiang Tan, Ruijun Liu, Xiaoke Chen, Jingxiang Wu, Yinggen Pan, Shun Lu, Walter Weder, Qingquan Luo
BACKGROUND: Delayed revascularization process and substitute infection remain to be key challenges in tissue engineered (TE) airway reconstruction. We propose an "in-vivo bioreactor" design, defined as an implanted TE substitutes perfused with an intra-scaffold medium flow created by an extracorporeal portable pump system for in situ organ regeneration. The perfusate keeps pre-seeded cells alive before revascularization. Meanwhile the antibiotic inside the perfusate controls topical infection...
January 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28197690/improvement-of-levan-production-in-bacillus-amyloliquefaciens-through-metabolic-optimization-of-regulatory-elements
#4
Yanyan Gu, Jiayi Zheng, Jun Feng, Mingfeng Cao, Weixia Gao, Yufen Quan, Yulei Dang, Yi Wang, Shufang Wang, Cunjiang Song
Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous promoters were evaluated for sacB expression, and the Pgrac promoter led to the highest level for both sacB transcription and levansucrase enzyme activity. The levan production in the corresponding recombinant strain ΔLP-pHTPgrac reached 30...
February 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28196102/inoculum-composition-determines-microbial-community-and-function-in-an-anaerobic-sequential-batch-reactor
#5
Allison R Perrotta, Rajkumari Kumaraswamy, Juan R Bastidas-Oyanedel, Eric J Alm, Jorge Rodríguez
The sustainable recovery of resources from wastewater streams can provide many social and environmental benefits. A common strategy to recover valuable resources from wastewater is to harness the products of fermentation by complex microbial communities. In these fermentation bioreactors high microbial community diversity within the inoculum source is commonly assumed as sufficient for the selection of a functional microbial community. However, variability of the product profile obtained from these bioreactors is a persistent challenge in this field...
2017: PloS One
https://www.readbyqxmd.com/read/28192217/the-relation-between-xyr1-overexpression-in-trichoderma-harzianum-and-sugarcane-bagasse-saccharification-performance
#6
Priscila da Silva Delabona, Gisele Nunes Rodrigues, Mariane Paludetti Zubieta, Jonas Ramoni, Carla Aloia Codima, Deise Juliana Lima, Cristiane Sanchez Farinas, José Geraldo da Cruz Pradella, Bernhard Seiboth
This work investigates the influence of the positive regulator XYR1 of Trichoderma harzianum on the production of cellulolytic enzymes, using sugarcane bagasse as carbon source. Constitutive expression of xyr1 was achieved under the control of the strong Trichoderma reesei pki1 promoter. Five clones with xyr1 overexpression achieved higher xyr1 expression and greater enzymatic productivity when cultivated under submerged fermentation, hence validating the genetic construction for T. harzianum. Clone 5 presented a relative expression of xyr1 26-fold higher than the parent strain and exhibited 66, 37, and 36% higher values for filter paper activity, xylanase activity, and β-glucosidase activity, respectively, during cultivation in a stirred-tank bioreactor...
February 9, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28191902/dynamic-regulation-of-metabolic-flux-in-engineered-bacteria-using-a-pathway-independent-quorum-sensing-circuit
#7
Apoorv Gupta, Irene M Brockman Reizman, Christopher R Reisch, Kristala L J Prather
Metabolic engineering of microorganisms to produce desirable products on an industrial scale can result in unbalanced cellular metabolic networks that reduce productivity and yield. Metabolic fluxes can be rebalanced using dynamic pathway regulation, but few broadly applicable tools are available to achieve this. We present a pathway-independent genetic control module that can be used to dynamically regulate the expression of target genes. We apply our module to identify the optimal point to redirect glycolytic flux into heterologous engineered pathways in Escherichia coli, resulting in titers of myo-inositol increased 5...
February 13, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28191785/impact-of-the-secretome-of-human-mesenchymal-stem-cells-on-brain-structure-and-animal-behavior-in-a-rat-model-of-parkinson-s-disease
#8
Fábio G Teixeira, Miguel M Carvalho, Krishna M Panchalingam, Ana J Rodrigues, Bárbara Mendes-Pinheiro, Sandra Anjo, Bruno Manadas, Leo A Behie, Nuno Sousa, António J Salgado
Research in the last decade strongly suggests that mesenchymal stem cell (MSC)-mediated therapeutic benefits are mainly due to their secretome, which has been proposed as a possible therapeutic tool for the treatment of Parkinson's disease (PD). Indeed, it has been shown that the MSC secretome increases neurogenesis and cell survival, and has numerous neuroprotective actions under different conditions. Additionally, using dynamic culturing conditions (through computer-controlled bioreactors) can further modulate the MSC secretome, thereby generating a more potent neurotrophic factor cocktail (i...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191779/development-of-a-three-dimensional-bioengineering-technology-to-generate-lung-tissue-for-personalized-disease-modeling
#9
Dan C Wilkinson, Jackelyn A Alva-Ornelas, Jennifer M S Sucre, Preethi Vijayaraj, Abdo Durra, Wade Richardson, Steven J Jonas, Manash K Paul, Saravanan Karumbayaram, Bruce Dunn, Brigitte N Gomperts
Stem cell technologies, especially patient-specific, induced stem cell pluripotency and directed differentiation, hold great promise for changing the landscape of medical therapies. Proper exploitation of these methods may lead to personalized organ transplants, but to regenerate organs, it is necessary to develop methods for assembling differentiated cells into functional, organ-level tissues. The generation of three-dimensional human tissue models also holds potential for medical advances in disease modeling, as full organ functionality may not be necessary to recapitulate disease pathophysiology...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191777/enhanced-expansion-and-sustained-inductive-function-of-skin-derived-precursor-cells-in-computer-controlled-stirred-suspension-bioreactors
#10
Natacha A Agabalyan, Breanna S Borys, Holly D Sparks, Kathryn Boon, Eko W Raharjo, Sepideh Abbasi, Michael S Kallos, Jeff Biernaskie
Endogenous dermal stem cells (DSCs) reside in the adult hair follicle mesenchyme and can be isolated and grown in vitro as self-renewing colonies called skin-derived precursors (SKPs). Following transplantation into skin, SKPs can generate new dermis and reconstitute the dermal papilla and connective tissue sheath, suggesting they could have important therapeutic value for the treatment of skin disease (alopecia) or injury. Controlled cell culture processes must be developed to efficiently and safely generate sufficient stem cell numbers for clinical use...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28188692/bioreactor-scale-up-and-protein-product-quality-characterization-of-piggybac-transposon-derived-cho-pools
#11
Yashas Rajendra, Sowmya Balasubramanian, Robert B Peery, James R Swartling, Neil A McCracken, Dawn L Norris, Christopher C Frye, Gavin C Barnard
Chinese hamster ovary (CHO) cells remain the most popular host for the production of biopharmaceutical drugs, particularly monoclonal antibodies (mAbs), bispecific antibodies and Fc-fusion proteins. Creating and characterizing the stable CHO clonally-derived cell lines (CDCLs) needed to manufacture these therapeutic proteins is a lengthy and laborious process. Therefore, CHO pools have increasingly been used to rapidly produce protein to support and enable preclinical drug development. We recently described the generation of CHO pools yielding mAb titers as high as 7...
February 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28188657/a-substance-exchanger-based-bioreactor-culture-of-pig-discs-for-studying-the-immature-nucleus-pulposus
#12
Pei Li, Yibo Gan, Haoming Wang, Yuan Xu, Lei Song, Liyuan Wang, Bin Ouyang, Qiang Zhou
Various research models have been developed to study the biology of disc cells. Recently, the adult disc nucleus pulposus (NP) has been well studied. However, the immature NP is underinvestigated due to a lack of a suitable model. This study aimed to establish an organ culture of immature porcine disc by optimizing culture conditions and using a self-developed substance exchanger-based bioreactor. Immature porcine discs were first cultured in the bioreactor for 7 days at various levels of glucose (low, medium, high), osmolarity (hypo-, iso-, hyper-) and serum (5, 10, 20%) to determine the respective optimal level...
February 11, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28188331/urnula-sp-an-endophyte-of-dicksonia-antarctica-making-a-fragrant-mixture-of-biologically-active-volatile-organic-compounds
#13
Gary Strobel, Amy Ericksen, Joe Sears, Jie Xie, Brad Geary, Bryan Blatt
Urnula sp. was isolated as an endophyte of Dicksonia antarctica and identified primarily on the basis of its ITS sequence and morphological features. The anamorphic state of the fungus appeared as a hyphomyceteous-like fungus as based on its features in culture and scanning electron microscopy examination of its spores. On potato dextrose agar (PDA), the organism makes a characteristic fragrance resembling peach pie with vanilla overtones. A GC/MS analysis done on the volatile organic compounds (VOCs) of this organism, trapped by carbotrap methodology, revealed over 150 compounds with high MS matching quality being noted for 44 of these...
February 10, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28187342/statistical-monitoring-and-dynamic-simulation-of-a-wastewater-treatment-plant-a-combined-approach-to-achieve-model-predictive-control
#14
Xiaodong Wang, Harsha Ratnaweera, Johan Abdullah Holm, Vibeke Olsbu
The on-line monitoring of Chemical oxygen demand (COD) and total phosphorus (TP) restrains wastewater treatment plants to achieve better control of aeration and chemical dosing. In this study, we applied principal components analysis (PCA) to find out significant variables for COD and TP prediction. Multiple regression method applied the variables suggested by PCA to predict influent COD and TP. Moreover, a model of full-scale wastewater treatment plant with moving bed bioreactor (MBBR) and ballasted separation process was developed to simulate the performance of wastewater treatment...
February 7, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28186124/algal-cell-response-to-pulsed-waved-stimulation-and-its-application-to-increase-algal-lipid-production
#15
Oleksandra Savchenko, Jida Xing, Xiaoyan Yang, Quanrong Gu, Mohamed Shaheen, Min Huang, Xiaojian Yu, Robert Burrell, Prabir Patra, Jie Chen
Generating renewable energy while sequestering CO2 using algae has recently attracted significant research attention, mostly directing towards biological methods such as systems biology, genetic engineering and bio-refining for optimizing algae strains. Other approaches focus on chemical screening to adjust culture conditions or culture media. We report for the first time the physiological changes of algal cells in response to a novel form of mechanical stimulation, or a pulsed wave at the frequency of 1.5 MHz and the duty cycle of 20%...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28185267/regulation-of-adipose-tissue-derived-stromal-cell-orientation-and-motility-in-2d-and-3d-cultures-by-direct-current-electrical-field
#16
Gang Yang, Haiyan Long, Xiaomei Ren, Kunlong Ma, Zhenghua Xiao, Ying Wang, Yingqiang Guo
Cell alignment and motility play a critical role in a variety of cell behaviors, including cytoskeleton reorganization, membrane-protein relocation, nuclear gene expression, and extracellular matrix remodeling. Direct current electric field (EF) in vitro can direct many types of cells to align vertically to EF vector. In this work, we investigated the effects of EF stimulation on rat adipose-tissue-derived stromal cells (ADSCs) in 2D-culture on plastic culture dishes and in 3D-culture on various scaffold materials, including collagen hydrogels, chitosan hydrogels and poly(L-lactic acid)/gelatin electrospinning fibers...
February 10, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28181412/aligned-nanofibrous-cell-derived-extracellular-matrix-for-anisotropic-vascular-graft-construction
#17
Qi Xing, Zichen Qian, Mitchell Tahtinen, Ai Hui Yap, Keegan Yates, Feng Zhao
There is a large demand for tissue engineered vascular grafts for the application of vascular reconstruction surgery or in vitro drug screening tissue model. The extracellular matrix (ECM) composition along with the structural and mechanical anisotropy of native blood vessels is critical to their functional performance. The objective of this study is to develop a biomimetic vascular graft recapitulating the anisotropic features of native blood vessels by employing nanofibrous aligned fibroblast-derived ECM and human mesenchymal stem cells (hMSCs)...
February 9, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28179160/tubular-collagen-scaffolds-with-radial-elasticity-for-hollow-organ-regeneration
#18
Luuk R Versteegden, Kenny A van Kampen, Heinz P Janke, Dorien M Tiemessen, Theo G Hafmans, Edwin A Roozen, Roger M Lomme, Harry van Goor, Egbert Oosterwijk, Wout F Feitz, Toin H van Kuppevelt, Willeke F Daamen
: Tubular collagen scaffolds have been used for the repair of damaged hollow organs in regenerative medicine, but they generally lack the ability to reversibly expand in radial direction, a physiological characteristic seen in many native tubular organs. In this study, tubular collagen scaffolds were prepared that display a shape recovery effect and therefore exhibit radial elasticity. Scaffolds were constructed by compression of fibrillar collagen around a star-shaped mandrel, mimicking folds in a lumen, a typical characteristic of empty tubular hollow organs, such as ureter or urethra...
February 5, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28178610/removal-of-pharmaceuticals-from-municipal-wastewater-by-aerated-submerged-attached-growth-reactors
#19
Y Flores Velázquez, P Mijaylova Nacheva
The performance of four aerated submerged attached growth bioreactors was studied for the removal of three pharmaceutical micro-pollutants (fluoxetine, mefenamic acid and metoprolol) from municipal wastewater. Two packing materials (polyethylene tapes and polyurethane cubes) were compared and the effects of different organic loads (3.0, 6.0, 9.0 and 12 gCOD m(-2) d(-1)) and of the effluent recirculation were investigated. The obtained solid retention times were in the range of 4-37 d. The reactors packed with polyurethane cubes allowed 11-26% higher biomass accumulation than the ones with polyethylene tapes and higher solid retention times...
February 5, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28178596/automated-2d-hplc-method-for-characterization-of-protein-aggregation-with-in-line-fraction-collection-device
#20
Abasha Williams, Erik K Read, Cyrus D Agarabi, Scott Lute, Kurt A Brorson
Monoclonal antibodies are mainly produced by mammalian cell culture, which due to its complexity, results in a wide range of product variants/isoforms. With the growing implementation of Quality by Design (QbD) and Process Analytical Technology (PAT) in drug manufacturing, monitoring and controlling quality attributes within a predefined range during manufacturing may provide added consistency to product quality. To implement these concepts, more robust analytical tools could reduce the time needed for monitoring quality attributes during upstream processing...
January 25, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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