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https://www.readbyqxmd.com/read/28820476/biosynthesis-of-s-adenosylmethionine-by-magnetically-immobilized-escherichia-coli-cells-highly-expressing-a-methionine-adenosyltransferase-variant
#1
Chunli Yin, Tao Zheng, Xin Chang
S-Adenosylmethionine (SAM) is a natural metabolite having important uses in the treatment of various diseases. To develop a simple and effective way to produce SAM, immobilized Escherichia coli cells highly expressing an engineered variant of methionine adenosyltransferase (MAT) were employed to synthesize SAM. The recombinant I303V MAT variant was successfully produced at approximately 900 mg/L in a 10-L bioreactor and exhibited significantly less product inhibition and had a four-fold higher specific activity (14...
August 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28818711/the-influence-of-solid-retention-time-on-ifas-mbr-systems-assessment-of-nitrous-oxide-emission
#2
Giorgio Mannina, Marco Capodici, Alida Cosenza, Vito Armando Laudicina, Daniele Di Trapani
The aim of the present study was to investigate the nitrous oxide (N2O) emissions from a moving bed based Integrated Fixed Film Activated Sludge (IFAS) - membrane bioreactor (MBR) pilot plant, designed according to the University of Cape Town (UCT) layout. The experimental campaign had a duration of 110 days and was characterized by three different sludge retention time (SRT) values (∞, 30 d and 15 d). Results highlighted that N2O concentrations decreased when the biofilm concentrations increased within the aerobic reactor...
August 14, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28818103/enhancement-of-poly-3-hydroxybutyrate-co-3-hydroxyvalerate-accumulation-in-arxula-adeninivorans-by-stabilization-of-production
#3
Mateusz Biernacki, Marek Marzec, Thomas Roick, Reinhard Pätz, Kim Baronian, Rüdiger Bode, Gotthard Kunze
BACKGROUND: In recent years the production of biobased biodegradable plastics has been of interest of researchers partly due to the accumulation of non-biodegradable plastics in the environment and to the opportunity for new applications. Commonly investigated are the polyhydroxyalkanoates (PHAs) poly(hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHB-V). The latter has the advantage of being tougher and less brittle. The production of these polymers in bacteria is well established but production in yeast may have some advantages, e...
August 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28818028/investigating-the-performance-of-an-anaerobic-baffled-bioreactor-abr-for-the-biodegradation-of-alkaline-surfactant-polymer-asp-in-oilfield-water
#4
Changli Liu, Rongshu Zhang, Hongyi Yang, Jie Zhang, Hongwei Wang, Le Chang, Yuanlong Zhan, Qijiang Xu
The anaerobic baffled reactor (ABR) was used to treat Alkaline-surfactant-polymer (ASP) flooding wastewater in Daqing oilfield. With the ABR hydraulic retention time (HRT) was reduced from 72 hours decreased to 24 hours, the bioreactor purification capability improved gradually. After the ABR running of 100 days, the removal rate of raw oil, suspended solid and surfactant reached 99.8%, 94.4% and 50%, respectively; alkali, polymer and viscosity was removed at a rate of about 16%, 7% and 20%, respectively. There were 39 kinds of organic material detected by GCMS in the original water sample, while only 12 kinds of organics were left in the ABR outfall...
August 18, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28816520/emergence-of-two-distinct-subpopulations-from-klebsiella-pneumoniae-grown-in-the-stimulated-microgravity-environment
#5
Haili Wang, Wenliang Li, Lixiao Gu, Xiaofang Gao, Bin Ni, Haiteng Deng, Ruifu Yang, Yanping Han
AIM: To isolate and characterize the two phenotypically distinct subpopulations from Klebsiella pneumoniae clonal cultures grown in the simulate microgravity environment. MATERIALS & METHODS: Here clonal culture of K. pneumoniae strain ATCC BAA-1705 was grown within a vertically rotating wall vessel bioreactor. Microscopic, colony staining, biofilm assays and quantitative proteomics were used to define the features of subpopulations. RESULTS: Two subpopulations were isolated based on colony appearance and bacterial morphology and indicated the different capability of biofilm formation and antibiotics resistance...
August 17, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28814755/membrane-fouling-induced-by-ahl-mediated-soluble-microbial-product-smp-formation-by-fouling-causing-bacteria-co-cultured-with-fouling-enhancing-bacteria
#6
So Ishizaki, Ryoichi Sugiyama, Satoshi Okabe
Membrane fouling still remains a major obstacle for wider applications of membrane bioreactor (MBR), which is mainly caused by soluble microbial products (SMP). Identification of key bacteria responsible for SMP production is essential for mitigation of membrane fouling. Here, we investigated the effect of microbial interaction on membrane fouling. We measured the membrane fouling potentials of 13 bacterial strains isolated from a pilot-scale MBR treating domestic wastewater when they were cultivated as single-culture and co-culture...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28812881/predicting-microbial-fuel-cell-biofilm-communities-and-bioreactor-performance-using-artificial-neural-networks
#7
Keaton Larson Lesnik, Hong Liu
The complex interactions that occur in mixed-species bioelectrochemical reactors, like microbial fuel cells (MFCs), make accurate predictions of performance outcomes under untested conditions difficult. While direct correlations between any individual waste stream characteristic or microbial community structure and reactor performance have not been able to be directly established, the increase in sequencing data and readily available computational power enables the development of alternate approaches. In the current study, 33 MFCs were evaluated under a range of conditions including 8 separate substrates and 3 different wastewaters...
August 16, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28812146/engineering-rhodosporidium-toruloides-for-the-production-of-very-long-chain-monounsaturated-fatty-acid-rich-oils
#8
Sandy Fillet, Carmen Ronchel, Carla Callejo, María-José Fajardo, Helena Moralejo, José L Adrio
Erucic acid (cis-docosa-13-enoic acid, C22:1∆13) and nervonic acid (cis-tetracosa-15-enoic acid, C24:1 ∆15) are important renewable feedstocks in plastic, cosmetic, nylon, and lubricant industries. Furthermore, nervonic acid is also applied to the treatment of some neurological diseases. However, the production of these two very long-chain fatty acids (VLCFA) is very limited as both are not present in the main vegetable oils (e.g., soybean, rapeseed, sunflower, and palm). Ectopic integration and heterologous expression of fatty acid elongases (3-ketoacyl-CoA synthases, KCS) from different plants in Rhodosporidium toruloides resulted in the de novo synthesis of erucic acid and nervonic acid in this oleaginous yeast...
August 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28811974/ex-vivo-assessment-of-drug-response-on-breast-cancer-primary-tissue-with-preserved-microenvironments
#9
Manuele G Muraro, Simone Muenst, Valentina Mele, Luca Quagliata, Giandomenica Iezzi, Alexandar Tzankov, Walter P Weber, Giulio C Spagnoli, Savas D Soysal
Interaction between cancerous, non-transformed cells, and non-cellular components within the tumor microenvironment plays a key role in response to treatment. However, short-term culture or xenotransplantation of cancer specimens in immunodeficient animals results in dramatic modifications of the tumor microenvironment, thus preventing reliable assessment of compounds or biologicals of potential therapeutic relevance. We used a perfusion-based bioreactor developed for tissue engineering purposes to successfully maintain the tumor microenvironment of freshly excised breast cancer tissue obtained from 27 breast cancer patients and used this platform to test the therapeutic effect of antiestrogens as well as checkpoint-inhibitors on the cancer cells...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28811146/n2o-emissions-from-an-intermittently-aerated-semi-aerobic-aged-refuse-bioreactor-combined-effect-of-cod-and-nh4-n-in-influent-leachate
#10
Weihua Li, Yingjie Sun, Rongxing Bian, Huawei Wang, Dalei Zhang
The carbon-nitrogen ratio (COD/NH4(+)-N) is an important factor affecting nitrification and denitrification in wastewater treatment; this factor also influences nitrous oxide (N2O) emissions. This study investigated two simulated intermittently aerated semi-aerobic aged refuse bioreactors (SAARB) filled with 8-year old aged refuse (AR). The research analyzed how differences in and the combination of influent COD and NH4(+)-N impact N2O emissions in leachate treatment. Experimental results showed that N2O emissions increased as the influent COD/NH4(+)-N decreased...
August 12, 2017: Waste Management
https://www.readbyqxmd.com/read/28810505/denitrification-of-the-anaerobic-membrane-bioreactor-anmbr-effluent-with-alternative-electron-donors-in-domestic-wastewater-treatment
#11
L Pelaz, A Gómez, G Garralón, A Letona, M Fdz-Polanco
A fixed film bioreactor for the denitrification of the effluent from an anaerobic membrane bioreactor (AnMBR) treating domestic wastewater was designed, built and investigated. After anaerobic treatment, the wastewater usually has a low C/N ratio (∼1.3), and a remaining chemical oxygen demand of around 117mg O2/L, which is not enough to make conventional heterotrophic denitrification possible. That effluent also holds methane and sulfide dissolved and oversaturated after leaving the AnMBR. This paper demonstrates the feasibility of using these reduced compounds as electron donors in order to remove 80mg NOx(-)-N/L at 18°C and 2h of hydraulic retention time...
July 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28810222/bio-electro-oxidation-of-indigo-carmine-by-using-microporous-activated-carbon-fiber-felt-as-anode-and-bioreactor-support
#12
Luane Ferreira Garcia, Ana Claudia Rodrigues Siqueira, Germán Sanz Lobón, Jossano Saldanha Marcuzzo, Benevides Costa Pessela, Eduardo Mendez, Telma Alves Garcia, Eric de Souza Gil
The bioremediation and electro-oxidation (EO) processes are included among the most promising cleaning and decontamination mechanisms of water. The efficiency of bioremediation is dictated by the biological actuator for a specific substrate, its suitable immobilization and all involved biochemical concepts. The EO performance is defined by the anode efficiency to perform the complete mineralization of target compounds and is highlighted by the low or null use of reagent. Recently, the combination of both technologies has been proposed...
August 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28808937/high-performance-biogas-upgrading-using-a-biotrickling-filter-and-hydrogenotrophic-methanogens
#13
Trisha L Dupnock, Marc A Deshusses
This research reports the development of a biotrickling filter (BTF) to upgrade biogas, which is achieved by adding H2 to reduce CO2. H2 and CO2 (80:20% vol.) were fed to a bench-scale BTF packed with polyurethane foam (PUF) and inoculated with hydrogenotrophic methanogens. Maximum CH4 production rates recorded were as high as 38 m(3)CH4 m(-3)reactor day(-1), which is 5-30 times faster than earlier reports with other kinds of bioreactors. The high rates were attributed to the efficient mass transfer and high density of methanogens in the BTF...
August 14, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28805306/bioprocess-scale-up-down-as-integrative-enabling-technology-from-fluid-mechanics-to-systems-biology-and-beyond
#14
Frank Delvigne, Ralf Takors, Rob Mudde, Walter van Gulik, Henk Noorman
Efficient optimization of microbial processes is a critical issue for achieving a number of sustainable development goals, considering the impact of microbial biotechnology in agrofood, environment, biopharmaceutical and chemical industries. Many of these applications require scale-up after proof of concept. However, the behaviour of microbial systems remains unpredictable (at least partially) when shifting from laboratory-scale to industrial conditions. The need for robust microbial systems is thus highly needed in this context, as well as a better understanding of the interactions between fluid mechanics and cell physiology...
August 14, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28803423/oxygen-mass-transfer-and-shear-stress-effects-on-pseudomonas-putida-bcrc-14365-growth-to-improve-bioreactor-design-and-performance
#15
Hamed Moradkhani, Mir-Shahabeddin Izadkhah, Navideh Anarjan, Abolfazl Abdi
In this work, the experimental evidence is presented for two basic issues including oxygen mass transfer and shear analysis on the microorganism containing medium on the most prominent sections of the bioreactor. Computational fluid dynamics (CFD) methodology reproduces shear rate values for specific impeller designs using the commercial code (Fluent 6.2). CFD calculates volumetric mass transfer coefficient based on the Higbie's penetration theory. Four types of impeller are used. The spherical probe is used to measure flow hydrodynamic parameters to obtain shear rate by electro-diffusion (ED) method...
August 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28803106/effects-of-fractal-roughness-of-membrane-surfaces-on-interfacial-interactions-associated-with-membrane-fouling-in-a-membrane-bioreactor
#16
Shushu Feng, Genying Yu, Xiang Cai, Mahoro Eulade, Hongjun Lin, Jianrong Chen, Yong Liu, Bao-Qiang Liao
Fractal roughness is one of the most important properties of a fractal surface. In this study, it was found that, randomly rough membrane surface was a fractal surface, which could be digitally modeled by a modified two-variable Weierstrass-Mandelbrot (WM) function. Fractal roughness of membrane surfaces has a typical power function relation with the statistical roughness of the modeled surface. Assessment of interfacial interactions showed that an increase in fractal roughness of membrane surfaces will strengthen and prolong the interfacial interactions between membranes and foulants, and under conditions in this study, will significantly increase the adhesion propensity of a foulant particle on membrane surface...
July 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28802825/the-role-of-nuclear-mechanics-in-cell-deformation-under-creeping-flows
#17
Francisco Serrano-Alcalde, José Manuel García-Aznar, María José Gómez-Benito
Despite the relevant regulatory role that nuclear deformation plays in cell behaviour, a thorough understanding of how fluid flow modulates the deformation of the cell nucleus in non-confined environments is lacking. In this work, we investigated the dynamics of cell deformation under different creeping flows as a general simulation tool for predicting nuclear stresses and strains. Using this solid-fluid modelling interaction framework, we assessed the stress and strain levels that the cell nucleus experiences as a function of different microenvironmental conditions, such as physical constraints, fluid flows, cytosol properties, and nucleus properties and size...
August 9, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28802137/development-of-novel-polysulfone-membranes-with-embedded-zirconium-sulfate-surfactant-micelle-mesostructure-for-phosphate-recovery-from-water-through-membrane-filtration
#18
Kenji Furuya, Akira Hafuka, Miho Kuroiwa, Hisashi Satoh, Yoshimasa Watanabe, Hiroshi Yamamura
We prepared novel membranes that could adsorb phosphate from water through membrane filtration for use in a phosphate recovery system. Zirconium sulfate surfactant micelle mesostructure (ZS), which was the phosphate adsorbent, was embedded in a polysulfone matrix and flat sheet ultrafiltration membranes were made by nonsolvent induced phase separation. Scanning electron microscopy showed that the ZS particles existed on both the top surface and in the internal surface of the membrane. Increases in ZS content led to greater pure water flux because of increases in the surface porosity ratio...
August 5, 2017: Water Research
https://www.readbyqxmd.com/read/28801873/transgenic-silkworms-secrete-the-recombinant-glycosylated-mrjp1-protein-of-chinese-honeybee-apis-cerana-cerana
#19
Zhengying You, Qiujie Qian, Yiran Wang, Jiaqian Che, Lupeng Ye, Lirong Shen, Boxiong Zhong
Major royal jelly protein-1 (MRJP1) is the most abundant glycoprotein of royal jelly (RJ) and is considered a potential component of functional foods. In this study, we used silkworm transgenic technology to obtain five transgenic silkworm lineages expressing the exogenous recombinant Chinese honeybee, Apis cerana cerana, protein-1 (rAccMRJP1) under the control of a fibroin light chain (Fib-L) promoter in the posterior silk glands. The protein was successfully secreted into cocoons; specifically, the highest rAccMRJP1 protein content was 0...
August 11, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28800557/predicting-and-experimental-evaluating-bio-electrochemical-synthesis-a-case-study-with-clostridium-kluyveri
#20
Christin Koch, Anne Kuchenbuch, Frauke Kracke, Paul V Bernhardt, Jens Krömer, Falk Harnisch
Microbial electrosynthesis is a highly promising application of microbial electrochemical technologies for the sustainable production of organic compounds. At the same time a multitude of questions need to be answered and challenges to be met. Central for its further development is using appropriate electroactive microorganisms and their efficient extracellular electron transfer (EET) as well as wiring of the metabolism to EET. Among others, Clostridia are believed to represent electroactive microbes being highly promising for microbial electrosynthesis...
July 25, 2017: Bioelectrochemistry
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