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https://www.readbyqxmd.com/read/28535613/expression-and-purification-of-biologically-active-human-bone-morphogenetic-protein-4-in-recombinant-chinese-hamster-ovary-cells
#1
Minyub Cha, Nara Han, Jia Pi, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon
Bone morphogenetic protein-4 (BMP-4) is considered to have therapeutic potential for various diseases including cancers; however, the high expression of biologically active recombinant human BMP-4 (rhBMP-4) needed to manufacture rhBMP-4 for therapeutic purposes has yet to be established. In the current study, we established a recombinant Chinese hamster ovary (rCHO) cell line overexpressing rhBMP-4 as well as a production process using 7.5-l bioreactor (5 l working volume). The expression of the mature rhBMP-4 was significantly enhanced by recombinant furin expression...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28534167/platforms-for-manufacturing-allogeneic-autologous-and-ipsc-cell-therapy-products-an-industry-perspective
#2
Eytan Abraham, Behnam Baghbaderani Ahmadian, Kathryn Holderness, Yonatan Levinson, Erika McAfee
As cell therapy processes mature from benchtop research protocols to industrial processes capable of manufacturing market-relevant numbers of doses, new cell manufacturing platforms are required. Here we give an overview of the platforms and technologies currently available to manufacture allogeneic cell products, such as mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs), and technologies for mass production of autologous cell therapies via scale-out. These technologies include bioreactors, microcarriers, cell separation and cryopreservation equipment, molecular biology tools for iPSC generation, and single-use controlled-environment systems for autologous cell production...
May 23, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28533815/efficient-itaconic-acid-production-from-glycerol-with-ustilago-vetiveriae-tz1
#3
Thiemo Zambanini, Hamed Hosseinpour Tehrani, Elena Geiser, Dorothee Merker, Sarah Schleese, Judith Krabbe, Joerg M Buescher, Guido Meurer, Nick Wierckx, Lars M Blank
BACKGROUND: The family of Ustilaginaceae is known for their capability to naturally produce industrially valuable chemicals from different carbon sources. Recently, several Ustilaginaceae were reported to produce organic acids from glycerol, which is the main side stream in biodiesel production. RESULTS: In this study, we present Ustilago vetiveriae as new production organism for itaconate synthesis from glycerol. In a screening of 126 Ustilaginaceae, this organism reached one of the highest titers for itaconate combined with a high-glycerol uptake rate...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28533063/photosynthetic-bacteria-based-membrane-bioreactor-as-post-treatment-of-an-anaerobic-membrane-bioreactor-effluent
#4
E González, O Díaz, I Ruigómez, C R de Vera, L E Rodríguez-Gómez, J Rodríguez-Sevilla, L Vera
Anaerobic membrane bioreactors have attracted increasing interest in the field of wastewater treatment. However, significant amounts of organic matter, nitrogen and sulphide in the effluent may limit its reuse. A photosynthetic bacteria-based membrane bioreactor is proposed for the further treatment of this effluent. A pilot-scale unit was run outdoor for over 900h to assess the process performance at short hydraulic retention time. After an initial biomass development, simultaneous removal of soluble organic matter and nitrogen was achieved (65% and 39%, respectively)...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28532145/mini-photobioreactors-for-in-vivo-real-time-characterization-and-evolutionary-tuning-of-bacterial-optogenetic-circuit
#5
Hsinkai Wang, Ya-Tang Yang
The current standard protocols for characterizing the optogenetic circuit of bacterial cells using flow cytometry in light tubes and light exposure of culture plates are tedious, labor-intensive, and cumbersome. In this work, we engineer a bioreactor with working volume of ~10 mL for in vivo real-time optogenetic characterization of E. coli with a CcaS-CcaR light-sensing system. In the bioreactor, optical density measurements, reporter protein fluorescence detection, and light input stimuli are provided by four light-emitting diode sources and two photodetectors...
May 22, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28531959/occurrence-and-removal-of-pharmaceuticals-hormones-personal-care-products-and-endocrine-disrupters-in-a-full-scale-water-reclamation-plant
#6
Ngoc Han Tran, Karina Yew-Hoong Gin
This study provided the first comprehensive data on the occurrence and removal of twenty-five target emerging contaminants (ECs) in a full-scale water reclamation plant (WRP) in the Southeast Asian region. Nineteen out of the twenty-five ECs were ubiquitously detected in raw influent samples. Concentrations of the detected ECs in raw influent samples ranged substantially from 44.3 to 124,966ng/L, depending upon the compound and sampling date. The elimination of ECs in full-scale conventional activated sludge (CAS) and membrane bioreactor (MBR) systems at a local WRP was evaluated and compared...
May 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28531862/unravelling-the-suitability-of-biological-induction-for-halophilic-lipase-production-by-halomonas-sp-lm1c-cultures
#7
Esther Gutiérrez-Arnillas, María Arellano, Francisco J Deive, Ana Rodríguez, María Ángeles Sanromán
In this study, the viability of using biological induction as an alternative to the conventional chemical induction in lipase production by a novel halophilic microorganism, Halomonas sp. LM1C, has been demonstrated. Thus, a 9-times increase of lipase activity (3000U/L) was recorded when Staphylococcus equorum sp. AMC7 was present in the medium, which is competitive with the results obtained when Triton X-100 was added as chemical inducer. The GC-MS data allowed concluding the true nature of the biological inducer effect, as the existence of high percentages of isomeric forms of pentadecanoic acid were detected...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531861/greenhouse-gases-from-membrane-bioreactors-mathematical-modelling-sensitivity-and-uncertainty-analysis
#8
Giorgio Mannina, Alida Cosenza, George A Ekama
In this study a new mathematical model to quantify greenhouse gas emissions (namely, carbon dioxide and nitrous oxide) from membrane bioreactors (MBRs) is presented. The model has been adopted to predict the key processes of a pilot plant with pre-denitrification MBR scheme, filled with domestic and saline wastewater. The model was calibrated by adopting an advanced protocol based on an extensive dataset. In terms of nitrous oxide, the results show that an important role is played by the half saturation coefficients related to nitrogen removal processes and the model factors affecting the oxygen transfer rate in the aerobic and MBR tanks...
May 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531853/methane-production-in-an-anaerobic-osmotic-membrane-bioreactor-using-forward-osmosis-effect-of-reverse-salt-flux
#9
Sheng Li, Youngjin Kim, Sherub Phuntsho, Laura Chekli, Ho Kyong Shon, TorOve Leiknes, Noreddine Ghaffour
This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO3 and KH2PO4 as draw solutes. Results showed that KH2PO4 exhibited the lowest RSF in terms of molar concentration 19.1mM/(m(2).h), while for KCl and KNO3 it was 32.2 and 120.8mM/(m(2).h), respectively. Interestingly, bio-methane production displayed an opposite order with KH2PO4, followed by KCl and KNO3. Pyrosequencing results revealed the presence of different bacterial communities among the tested fertilizers...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28531557/characterization-of-dissolved-organic-carbon-leached-from-a-woodchip-bioreactor
#10
Ibrahim Abusallout, Guanghui Hua
Woodchip bioreactors are increasingly being applied to remove nitrate from agricultural subsurface drainage. However, dissolved organic carbon (DOC) released from woodchips may negatively affect the aquatic ecosystems and drinking water supplies. The objective of this study was to evaluate the leaching characteristics, disinfection byproduct (DBP) formation potentials, and treatability of DOC derived from a laboratory woodchip bioreactor. Initial flush of woodchips resulted in the release of high organic content from woodchips...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28529639/overexpression-of-insulin-like-growth-factor-1-enhanced-the-osteogenic-capability-of-aging-bone-marrow-mesenchymal-stem-cells
#11
Ching-Yun Chen, Kuo-Yun Tseng, Yen-Liang Lai, Yo-Shen Chen, Feng-Huei Lin, Shankung Lin
Many studies have indicated that loss of the osteoblastogenic potential in bone marrow mesenchymal stem cells (bmMSCs) is the major component in the etiology of the aging-related bone deficit. But how the bmMSCs lose osteogenic capability in aging is unclear. Using 2-dimentional cultures, we examined the dose response of human bmMSCs, isolated from adult and aged donors, to exogenous insulin-like growth factor 1 (IGF-1), a growth factor regulating bone formation. The data showed that the mitogenic activity and the osteoblastogenic potential of bmMSCs in response to IGF-1 were impaired with aging, whereas higher doses of IGF-1 increased the proliferation rate and osteogenic potential of aging bmMSCs...
2017: Theranostics
https://www.readbyqxmd.com/read/28528699/steps-toward-maturation-of-embryonic-stem-cell-derived-cardiomyocytes-by-defined-physical-signals
#12
Nian Shen, Anne Knopf, Claas Westendorf, Udo Kraushaar, Julia Riedl, Hannah Bauer, Simone Pöschel, Shannon Lee Layland, Monika Holeiter, Stefan Knolle, Eva Brauchle, Ali Nsair, Svenja Hinderer, Katja Schenke-Layland
Cardiovascular disease remains a leading cause of mortality and morbidity worldwide. Embryonic stem cell-derived cardiomyocytes (ESC-CMs) may offer significant advances in creating in vitro cardiac tissues for disease modeling, drug testing, and elucidating developmental processes; however, the induction of ESCs to a more adult-like CM phenotype remains challenging. In this study, we developed a bioreactor system to employ pulsatile flow (1.48 mL/min), cyclic strain (5%), and extended culture time to improve the maturation of murine and human ESC-CMs...
May 18, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28528488/enhanced-production-of-para-hydroxybenzoic-acid-by-genetically-engineered-saccharomyces-cerevisiae
#13
Nils J H Averesch, Alex Prima, Jens O Krömer
Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized...
May 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28527827/in%C3%A2-vitro-bioconversion-of-chitin-to-pyruvate-with-thermophilic-enzymes
#14
Kohsuke Honda, Keisuke Kimura, Pham Huynh Ninh, Hironori Taniguchi, Kenji Okano, Hisao Ohtake
Chitin is the second most abundant organic compound on the planet and thus has been regarded as an alternative resource to petroleum feedstocks. One of the key challenges in the biological conversion of biomass-derived polysaccharides, such as cellulose and chitin, is to close the gap between optimum temperatures for enzymatic saccharification and microbial fermentation and to implement them in a single bioreactor. To address this issue, in the present study, we aimed to perform an in vitro, one-pot bioconversion of chitin to pyruvate, which is a precursor of a wide range of useful metabolites...
May 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28526091/a-pre-therapeutic-coating-for-medical-devices-that-prevents-the-attachment-of-candida-albicans
#15
Diego Vargas-Blanco, Aung Lynn, Jonah Rosch, Rony Noreldin, Anthony Salerni, Christopher Lambert, Reeta P Rao
BACKGROUND: Hospital acquired fungal infections are defined as "never events"-medical errors that should never have happened. Systemic Candida albicans infections results in 30-50% mortality rates. Typically, adhesion to abiotic medical devices and implants initiates such infections. Efficient adhesion initiates formation of aggressive biofilms that are difficult to treat. Therefore, inhibitors of adhesion are important for drug development and likely to have a broad spectrum efficacy against many fungal pathogens...
May 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28525785/denitrifying-woodchip-bioreactor-and-phosphorus-filter-pairing-to-minimize-pollution-swapping
#16
Laura E Christianson, Christine Lepine, Philip L Sibrell, Chad Penn, Steven T Summerfelt
Pairing denitrifying woodchip bioreactors and phosphorus-sorbing filters provides a unique, engineered approach for dual nutrient removal from waters impaired with both nitrogen (N) and phosphorus (P). This column study aimed to test placement of two P-filter media (acid mine drainage treatment residuals and steel slag) relative to a denitrifying system to maximize N and P removal and minimize pollution swapping under varying flow conditions (i.e., woodchip column hydraulic retention times (HRTs) of 7.2, 18, and 51 h; P-filter HRTs of 7...
May 11, 2017: Water Research
https://www.readbyqxmd.com/read/28523446/in-vitro-quantification-of-luminal-denudation-after-crimping-and-balloon-dilatation-of-endothelialized-covered-stents
#17
Shigeo Ichihashi, Frederic Wolf, Thomas Schmitz-Rode, Kimihiko Kichikawa, Stefan Jockenhoevel, Petra Mela
PURPOSE: Covered stents have been demonstrated to reduce restenosis; however, the membrane's limited biocompatibility can still lead to thrombus formation. To obtain optimal surface hemocompatibility, endothelialization of the luminal surface has been proposed. However, the effect of delivery procedures, such as crimping and balloon dilatation, on the endothelial layer has not been quantified. This study investigated the impact of such procedures on endothelialized covered stents in vitro...
May 18, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28522999/fermentation-ph-modulates-the-size-distributions-and-functional-properties-of-gluconobacter-albidus-tmw-2-1191-levan
#18
Tharalinee Ua-Arak, Frank Jakob, Rudi F Vogel
Bacterial levan has gained an increasing interest over the last decades due to its unique characteristics and multiple possible applications. Levan and other exopolysaccharides (EPSs) production are usually optimized to obtain the highest concentration or yield while a possible change of the molecular size and mass during the production process is mostly neglected. In this study, the molar mass and radius of levan samples were monitored during fermentations with the food-grade, levan-producing acetic acid bacterium Gluconobacter (G...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28521233/fouling-reduction-using-adsorbents-flocculants-in-a-submerged-anaerobic-membrane-bioreactor
#19
Qiaoyun Zhang, Suniti Singh, David C Stuckey
Using adsorbents/flocculants in anaerobic membrane bioreactors (AnMBRs) to reduce membrane fouling is comparatively rare. This study evaluated 8 additives: 3 powdered activated carbons, 2 granular activated carbons, 1 cationic polymer, and 2 metal salts to identify the best additive and dose to minimise membrane fouling. Small cross flow filtration tests showed 400mg/L PAC SAE2, or 150mg/L FeCl3, reduced the transmembrane pressure (TMP) rise from 0.94 to 0.06kPa/h, indicating excellent fouling reduction. The best filtration performance correlated with a significant reduction in supernatant supracolloidal particles, colloids and SMPs...
May 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521227/start-up-and-operation-of-an-aerobic-granular-sludge-system-under-low-working-temperature-inoculated-with-cold-adapted-activated-sludge-from-finland
#20
Alejandro Gonzalez-Martinez, Barbara Muñoz-Palazon, Alejandro Rodriguez-Sanchez, Paula Maza-Márquez, Anna Mikola, Jesus Gonzalez-Lopez, Riku Vahala
An aerobic granular sludge system has been started-up and operated at 7°C temperature using cold-adapted activated sludge as inoculum. The system could form granular biomass due to batch operation allowing for just 5-3min of biomass sedimentation. Scanning electron microscopy showed that fungi helped in the granular biomass formation in the early stages of the granule formation. The removal performance of the system was of 92-95% in BOD5, 75-80% in COD, 70-76% in total nitrogen and 50-60% in total phosphorous...
May 10, 2017: Bioresource Technology
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