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https://www.readbyqxmd.com/read/28108411/production-and-characterization-of-medium-chain-length-polyhydroxyalkanoates-copolymer-from-arctic-psychrotrophic-bacterium-pseudomonas-sp-pamc-28620
#1
Ganesan Sathiyanarayanan, Shashi Kant Bhatia, Hun-Suk Song, Jong-Min Jeon, Jun-Young Kim, Yoo Kyung Lee, Yun-Gon Kim, Yung-Hun Yang
Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620 was found to produce a distinctive medium-chain-length polyhydroxyalkanoates (MCL-PHAs) copolymer when grown on structurally unrelated carbon sources including glycerol. The maximum MCL-PHAs copolymer yield was obtained about 52.18±4.12% from 7.95±0.66g/L of biomass at 144h of fermentation when 3% glycerol was used as sole carbon and energy source during the laboratory-scale bioreactor process. Characterization of the copolymer was carried out using fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC-MS), proton ((1)H) and carbon ((13)C) nuclear magnetic resonance spectroscopy (NMR), gel permeation chromatography (GPC), differential scanning calorimeter (DSC) and thermo-gravimetric analysis (TGA)...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108074/photo-fermentative-hydrogen-production-from-crop-residue-a-mini-review
#2
REVIEW
Quanguo Zhang, Yi Wang, Zhiping Zhang, Duu-Jong Lee, Xuehua Zhou, Yanyan Jing, Xumeng Ge, Danping Jiang, Jianjun Hu, Chao He
Photofermentative hydrogen production from crop residues, if feasible, can lead to complete conversion of organic substances to hydrogen (and carbon dioxide). This mini review lists the studies on photofermentative hydrogen production using crop residues as feedstock. Pretreatment methods, substrate structure, mechanism of photosynthetic bacteria growth and metabolism were discussed. Photofermentative hydrogen production from pure culture, consortia and mutants, and the geometry, light sources, mass transfer resistances and the operational strategies of the photo-bioreactor were herein reviewed...
January 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28107747/diversity-of-dna-viruses-in-effluents-of-membrane-bioreactors-in-traverse-city-mi-usa-and-la-grande-motte-france
#3
Evan O'Brien, Mariya Munir, Terence Marsh, Marc Heran, Geoffroy Lesage, Volodymyr V Tarabara, Irene Xagoraraki
This study assesses diversity of DNA viruses in the effluents of two membrane bioreactor (MBR) wastewater treatment plants (WWTPs): an MBR in the United States and an MBR in France. Viral diversity of these effluents is compared to that of a conventional activated sludge WWTP in the U.S. Diversity analysis indicates Herpesvirales to be the most abundant order of potentially pathogenic human DNA viruses in wastewater treated effluent in all utilities. Other potentially pathogenic human viruses detected include Adenoviridae, Parvoviridae, and Polyomaviridae...
January 8, 2017: Water Research
https://www.readbyqxmd.com/read/28106814/genome-analysis-of-a-novel-broad-host-range-proteobacteria-phage-isolated-from-a-bioreactor-treating-industrial-wastewater
#4
Marina de Leeuw, Maayan Baron, Asher Brenner, Ariel Kushmaro
Bacteriophages are viruses that infect bacteria, and consequently they have a major impact on the development of a microbial population. In this study, the genome of a novel broad host range bacteriophage, Aquamicrobium phage P14, isolated from a wastewater treatment plant, was analyzed. The Aquamicrobium phage P14 was found to infect members of different Proteobacteria classes (Alphaproteobacteria and Betaproteobacteria). This phage contains a 40,551 bp long genome and 60% of its genes had blastx hits. Furthermore, the bacteriophage was found to share more than 50% of its genes with several podoviruses and has the same gene order as other polyvalent bacteriophages...
January 18, 2017: Genes
https://www.readbyqxmd.com/read/28104552/a-novel-salt-tolerant-bacterial-consortium-for-biodegradation-of-saline-and-recalcitrant-petrochemical-wastewater
#5
Mehdi Ahmadi, Sahand Jorfi, Raheleh Kujlu, Shokouh Ghafari, Reza Darvishi Cheshmeh Soltani, Nematollah Jaafarzadeh Haghighifard
Treatment of a saline petrochemical wastewater with BOD5/COD ratio of less than 0.1 was investigated using a consortium consisted of three isolated salt-tolerant bacteria namely, Kocuria turfanesis, Halomonas alkaliphila and Pseudomonas balearica. Selected bacteria were isolated from petrochemical wastewater containing mineral salt mediums of 3% salinity. A lab-scale activated sludge bioreactor was used for startup in batch mode operation and after obtaining the MLSS concentration of about 3000 mg/L, the operation was changed to continuous flow mode to determine the biokinetic coefficients under different organic loading rates of 0...
January 16, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28104124/enhancing-survival-of-human-hepatocytes-by-neonatal-thymectomy-and-partial-hepatectomy-in-micro-miniature-pigs
#6
H C Hsu, S Enosawa, T Yamazaki, S Tohyama, J Fujita, K Fukuda, E Kobayashi
BACKGROUND: With the goal of in vivo cultivation of human hepatocytes that have not been sufficient in full differentiation in vitro, the advantage of neonatal thymectomy was verified on expansion of xenogeneic human hepatocyte in the micro-miniature pig (MMP). METHODS: The thymus was excised immediately after the birth of the MMPs via cesarean section. Newborns were fed by artificial feeding under specific pathogen-free conditions. The thymectomized and nonthymectomized littermates were transplanted with human hepatocytes via a portal vein with or without partial hepatectomy at the MMP adult stage...
January 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28102518/quercetin-glucoside-production-by-engineered-escherichia-coli
#7
REVIEW
Tian Xia, Mark A Eiteman
Escherichia coli strains expressing the O-glucosyltransferases UGT73B3 or UGT84B1 were compared for the production of glucosides from quercetin supplied into a defined medium. The formation of quercetin-3-glucoside (Q3G) by UGT73B3 showed a maximum at 33 °C, while the formation of quercetin-7-glucoside by UGT84B1 increased with increasing temperature to 37 °C. The highest concentrations of Q3G were attained by strains having a deletion in the pgi gene-coding phosphoglucose isomerase, which effectively blocked the entry of glucose-6P into the Embden-Meyerhof-Parnas pathway...
January 19, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28101787/a-recombinant-highly-thermostable-%C3%AE-mannanase-retman26-from-thermophilic-bacillus-subtilis-tbs2-expressed-in-pichia-pastoris-and-its-ph-and-temperature-stability
#8
Zhangcai Luo, Jing Miao, Guoying Li, Yao Du, Xiaobin Yu
A gene encoding a highly thermostable β-mannanase from a thermophilic Bacillus subtilis (TBS2) was successfully expressed in Pichia pastoris. The maximum activity of the recombinant thermostable β-mannanase (ReTMan26) was 5435 U/mL, which was obtained by high-density, fed-batch cultivation after 168-h induction with methanol in a 50-L bioreactor. The protein yield reached 3.29 mg/mL, and the protein had a molecular weight of ~42 kDa. After fermentation, ReTMan26 was purified using a 10-kDa cut-off membrane and Sephadex G-75 column...
January 18, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28097956/biohydrogen-production-from-used-diapers-evaluation-of-effect-of-temperature-and-substrate-conditioning
#9
P X Sotelo-Navarro, H M Poggi-Varaldo, S J Turpin-Marion, A Vázquez-Morillas, M Beltrán-Villavicencio, R M Espinosa-Valdemar
This research assessed the viability to use disposable diapers as a substrate for the production of biohydrogen, a valuable clean-energy source. The important content of cellulose of disposable diapers indicates that this waste could be an attractive substrate for biofuel production. Two incubation temperatures (35 °C and 55 °C) and three diaper conditioning methods (whole diapers with faeces, urine, and plastics, WD; diapers without plastic components, with urine and faeces, DWP; diapers with urine but without faeces and plastic, MSD) were tested in batch bioreactors...
January 1, 2017: Waste Management & Research
https://www.readbyqxmd.com/read/28095530/the-anti-aging-effects-of-low-oxygen-tension-generated-multipotent-growth-factor-containing-serum
#10
Zoe Diana Draelos, Jwala Karnik, Gail Naughton
Growth factors are a new category of ingredient found in modern cosmeceutical formulations. One novel method of obtaining cosmeceutical growth factors is the use of a bioreactor to culture neonatal broblasts on dextran microcarrier beads for 8 weeks under low oxygen tension (1-5%) mimicking embryonic conditions and eliminating the need for fetal bovine serum constituents in the final cosmetic material. This research evaluated the ingredient in a moisturizing vehicle on 40 females to determine its efficacy in improving overall facial skin appearance, as well as skin brightness, evenness, firmness, pore size, radiance, fine lines, coarse wrinkles, and blotchiness/ dispigmentation...
January 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28093887/collagen-hydrogel-scaffold-promotes-mesenchymal-stem-cell-and-endothelial-cell-coculture-for-bone-tissue-engineering
#11
Bao-Ngoc B Nguyen, Rebecca A Moriarty, Tim Kamalitdinov, Julie M Etheridge, John P Fisher
The generation of functional, vascularized tissues is a key challenge for the field of tissue engineering. Before clinical implantations of such tissue engineered bone constructs can succeed, tactics to promote neovascularization need to be strengthened. We have previously demonstrated that the tubular perfusion system (TPS) bioreactor is an effective culturing method to augment osteogenic differentiation and maintain viability of human mesenchymal stem cells (hMSC). Here, we devised a strategy to address the need for a functional microvasculature by designing an in vitro coculture system that simultaneously cultures osteogenic differentiating hMSCs with endothelial cells (ECs)...
January 17, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28093648/predicting-cell-viability-within-tissue-scaffolds-under-equiaxial-strain-multi-scale-finite-element-model-of-collagen-cardiomyocytes-constructs
#12
Mostafa Elsaadany, Karen Chang Yan, Eda Yildirim-Ayan
Successful tissue engineering and regenerative therapy necessitate having extensive knowledge about mechanical milieu in engineered tissues and the resident cells. In this study, we have merged two powerful analysis tools, namely finite element analysis and stochastic analysis, to understand the mechanical strain within the tissue scaffold and residing cells and to predict the cell viability upon applying mechanical strains. A continuum-based multi-length scale finite element model (FEM) was created to simulate the physiologically relevant equiaxial strain exposure on cell-embedded tissue scaffold and to calculate strain transferred to the tissue scaffold (macro-scale) and residing cells (micro-scale) upon various equiaxial strains...
January 16, 2017: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/28092500/a-model-of-a-synthetic-biological-communication-interface-between-mammalian-cells-and-mechatronic-systems
#13
Keith C Heyde, Warren C Ruder
The creation of communication interfaces between abiotic and biotic systems represents a significant research challenge. In this work, we design and model a system linking the biochemical signaling pathways of mammalian cells to the actions of a mobile robotic prosthesis. We envision this system as a robotic platform carrying an optically monitored bioreactor that harbors mammalian cells. The cellular, optical signal is captured by an onboard fluorescent microscope and converted into an electronic signal. We first present a design for the overall cell-robot system, with a specific focus on the design of the synthetic gene networks needed for the system...
December 2016: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/28089874/mathematical-modelling-of-cell-layer-growth-in-a-hollow-fibre-bioreactor
#14
Lloyd A C Chapman, Jonathan P Whiteley, Helen M Byrne, Sarah L Waters, Rebecca J Shipley
Generating autologous tissue grafts of a clinically useful volume requires efficient and controlled expansion of cell populations harvested from patients. Hollow fibre bioreactors show promise as cell expansion devices, owing to their potential for scale-up. However, further research is required to establish how to specify appropriate hollow fibre bioreactor operating conditions for expanding different cell types. In this study we develop a simple model for the growth of a cell layer seeded on the outer surface of a single fibre in a perfused hollow fibre bioreactor...
January 12, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28089204/effect-of-low-aeration-rate-on-simultaneous-nitrification-and-denitrification-in-an-intermittent-aeration-aged-refuse-bioreactor-treating-leachate
#15
Xiaojie Sun, Hongxia Zhang, Zhaowen Cheng, Siming Wang
Three intermittent aeration aged refuse bioreactors (ARBs), A, B, and C, with aeration rates of 670, 1340, and 2010L/m(3) aged refuse·d in stage 1, and 670, 503, and 335L/m(3) aged refuse·d in stage 2 were constructed to evaluate the effect of low aeration rate on leachate treatment by simultaneous nitrification and denitrification (SND). Results show that SND can be achieved and improved by reasonably adjusting the aeration rate of the ARB. In stage 1, the average chemical oxygen demand (COD) removal rates of ARBs A, B, and C were 91%, 92%, and 93%, respectively...
January 11, 2017: Waste Management
https://www.readbyqxmd.com/read/28089070/steric-exclusion-chromatography-for-purification-of-cell-culture-derived-influenza-a-virus-using-regenerated-cellulose-membranes-and-polyethylene-glycol
#16
Pavel Marichal-Gallardo, Michael M Pieler, Michael W Wolff, Udo Reichl
Steric exclusion chromatography has been used for the purification of proteins and bacteriophages using monoliths. The operation is carried out by mixing a crude sample containing the target species with a predetermined concentration and molecular weight of polyethylene glycol (PEG) and loading it onto a non-reactive hydrophilic surface. Product capture occurs by the mutual steric exclusion of PEG between the product and the matrix. Selectivity is significantly influenced by target product size. Product elution is achieved by decreasing the PEG concentration...
December 29, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28079174/alleviating-liver-failure-conditions-using-an-integrated-hybrid-cryogel-based-cellular-bioreactor-as-a-bioartificial-liver-support
#17
Apeksha Damania, Mohsin Hassan, Nana Shirakigawa, Hiroshi Mizumoto, Anupam Kumar, Shiv K Sarin, Hiroyuki Ijima, Masamichi Kamihira, Ashok Kumar
Conventionally, some bioartificial liver devices are used with separate plasmapheresis unit to separate out plasma from whole blood and adsorbent column to detoxify plasma before it passes through a hepatocytes-laden bioreactor. We aim to develop a hybrid bioreactor that integrates the separate modules in one compact design improving the efficacy of the cryogel based bioreactor as a bioartificial liver support. A plasma separation membrane and an activated carbon cloth are placed over a HepG2-loaded cryogel scaffold in a three-chambered bioreactor design...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28078826/the-impact-of-ph-inhomogeneities-on-cho-cell-physiology-and-fed-batch-process-performance-two-compartment-scale-down-modelling-and-intracellular-ph-excursion
#18
Matthias Brunner, Philipp Braun, Philipp Doppler, Christoph Posch, Dirk Behrens, Christoph Herwig, Jens Fricke
Due to high mixing times and base addition from top of the vessel, pH inhomogeneities are most likely to occur during large-scale mammalian processes. The goal of this study was to set-up a scale-down model of a 10-12 m(3) stirred tank bioreactor and to investigate the effect of pH perturbations on CHO cell physiology and process performance. Short-term changes in extracellular pH were hypothesized to affect intracellular pH and thus cell physiology. Therefore, batch fermentations, including pH shifts to 9...
January 12, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28078417/covalent-organic-framework-coated-magnetic-graphene-as-a-novel-support-for-trypsin-immobilization
#19
Heping Wang, Fenglong Jiao, Fangyuan Gao, Xinyuan Zhao, Yan Zhao, Yehua Shen, Yangjun Zhang, Xiaohong Qian
Deep and efficient proteolysis is the critical premise in mass spectrometry-based bottom-up proteomics. It is difficult for traditional in-solution digestion to meet the requirement unless prolonged digestion time and enhanced enzyme dosage are employed, which makes the whole workflow time-consuming and costly. The abovementioned problems could be effectively ameliorated by anchoring many proteases on solid supports. In this work, covalent organic framework-coated magnetic graphene (MG@TpPa-1) was designed and prepared as a novel enzyme carrier for the covalent immobilization of trypsin with a high degree of loading (up to 268 μg mg(-1))...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28074222/regulation-of-membrane-fixation-and-energy-production-conversion-for-adaptation-and-recovery-of-zno-nanoparticle-impacted-nitrosomonas-europaea
#20
Junkang Wu, Huijie Lu, Guangcan Zhu, Lianghui Chen, Yan Chang, Ran Yu
The ZnO nanoparticle (NP) effects on typical ammonia-oxidizing bacteria, Nitrosomonas europaea in a chemostat bioreactor, and the cells' toxicity adaptation and recovery potentials were explored. Hardly any inhibition was observed when the NP concentration was high up to 10 mg/L. The cells exposed to 50 mg/L ZnO NPs displayed time-dependent impairment and recovery potentials in terms of cell density, membrane integrity, nitrite production rate, and ammonia monooxygenase activity. The 6-h NP stress impaired cells were nearly completely restored during a 12-h recovery incubation, while the longer exposure time would cause irretrievable cell damage...
January 10, 2017: Applied Microbiology and Biotechnology
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