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https://www.readbyqxmd.com/read/28330302/production-of-bioherbicide-by-phoma-sp-in-a-stirred-tank-bioreactor
#1
Thiarles Brun, Jéssica E Rabuske, Izelmar Todero, Thiago C Almeida, Jair J D Junior, Gustavo Ariotti, Tássia Confortin, Jonas A Arnemann, Raquel C Kuhn, Jerson V C Guedes, Marcio A Mazutti
The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for bioherbicide production. Fermented broth was fractioned with different solvents to identify the molecules produced by the fungus in a multi-dimensional gas chromatograph system...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330223/multi-response-optimization-for-enhanced-xylitol-production-by-debaryomyces-nepalensis-in-bioreactor
#2
J Sharon Mano Pappu, Sathyanarayana N Gummadi
In this study, the optimization of different process variables-pH (4-6), aeration rate (200-550 rpm) and agitation rate (0.6-1.8 vvm) were investigated using rotating simplex method and uniform design method to enhance xylitol production from xylose by D. nepalensis in a batch stirred tank bioreactor. Maximum xylitol productivity (0.576 g L(-1) h(-1)) was obtained at pH 4.0, agitation 300 rpm and aeration 1.5 vvm by rotating simplex method. Individual optimum values of pH, agitation and aeration are 4...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330209/biodegradation-of-real-petroleum-wastewater-by-immobilized-hyper-phenol-tolerant-strains-of-bacillus-cereus-in-a-fluidized-bed-bioreactor
#3
Aditi Banerjee, Aloke K Ghoshal
Microbial bioremediation of petroleum wastewater by phenol-degrading-bacteria holds promise in circumventing the issue of petroleum-spill related pollution. Herein, biodegradation of petroleum wastewater samples collected from oil refinery site was carried out in a fluidized bed bioreactor by Ca-alginate immobilized biomass of phenol-degrading strains of Bacillus cereus (AKG1 MTCC9817 and AKG2 MTCC9818). Degradation performance of the system was evaluated by measuring the changes in chemical oxygen demand (COD) and level of phenolic compounds in the wastewater samples during the microbial treatment...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330201/dual-action-of-chromium-reducing-and-nitrogen-fixing-bacillus-megaterium-asnf3-for-improved-agro-rehabilitation-of-chromium-stressed-soils
#4
Sumaira Aslam, Ali Hussain, Javed Iqbal Qazi
We conducted a study for enhanced biological rehabilitation of chromium-contaminated soils using a chromium-reducing and nitrogen-fixing bacterial species (Bacillus megaterium-ASNF3). The bacterial species was isolated from a chromium-rich land area, characterized, and employed under optimum conditions for the treatment of artificially prepared chromium-rich soil. The bacterium reduced Cr(VI) up to 86 % in a 60-day trial of incubation in the soil bioreactor. The nitrogenase activity of the bacterium yielded up to 486 nmol of ethylene/mL/h after an incubation period of 40 days when it was optimally cultured in growth medium at neutral pH and 30 °C...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330191/biodegradation-of-4-chlorophenol-in-an-airlift-inner-loop-bioreactor-with-mixed-consortium-effect-of-hrt-loading-rate-and-biogenic-substrate
#5
Bhishma P Patel, Arvind Kumar
In the present work, removal of 4-chlorophenol (4-CP) by the mixed microbial consortium was evaluated in an airlift inner loop bioreactor. During the study, the effect of various reactor parameters such as hydraulic retention time (HRT), biogenetic substrate concentration, loading rate, and initial substrate concentration on the removal efficiency of 4-CP was investigated. Bioreactor showed a maximum removal rate of 16.59 mg/L/h at the optimum conditions of 24 h HRT, 400 mg/L initial 4-CP, and 0.2 g/L peptone...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330166/a-molecular-phylogenetic-framework-for-bacillus-subtilis-using-genome-sequences-and-its-application-to-bacillus-subtilis-subspecies-stecoris-strain-d7xpn1-an-isolate-from-a-commercial-food-waste-degrading-bioreactor
#6
Joseph Adelskov, Bharat K C Patel
A thermophilic, heterotrophic and facultatively anaerobic bacterium designated strain D7XPN1 was isolated from Baku BakuKing™, a commercial food-waste degrading bioreactor (composter). The strain grew optimally at 45 °C (growth range between 24 and 50 °C) and pH 7 (growth pH range between pH 5 and 9) in Luria Broth supplemented with 0.3 % glucose. Strain D7XPN1 tolerated up to 7 % NaCl and showed amylolytic and xylanolytic activities. 16S rRNA gene analysis placed strain D7XPN1 in the cluster represented by Bacillus subtilis and the genome analysis of the 4...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330140/statistical-based-optimization-and-scale-up-of-siderophore-production-process-on-laboratory-bioreactor
#7
S S Shaikh, S J Wani, R Z Sayyed
We report the enhanced production of siderophore in succinate medium by applying two-stage statistical approach, i.e., Plackett-Burman design and response surface methodology (RSM) using central composite design (CCD). In the first stage of optimization, out of 11 variable components of succinate medium, succinic acid, pH and temperature were found as significant components that influenced the siderophore production in Pseudomonas aeruginosa RZS9. The second stage of RSM using CCD consisted of optimizing the concentrations of the variables...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327257/determining-the-mechanism-for-low-sludge-yields-in-the-cannibal%C3%A2-solids-reduction-system
#8
Steven W Van Ginkel Joseph Miceli Bi-O Kim Ziming Yang Michelle Young Andrew Marcus And Bruce E Rittmann
Extracellular polymeric substances (EPS) and solids concentrations in samples from the Interchange Bioreactor (IBR) andreturn activated sludge (RAS) from Cannibal® plants having low and high sludge yields were analyzed to understand the mechanisms behind low sludge production. Low sludge yields correlated to more EPS degradation, higher concentrations of iron, and reducing conditions in the IBR. In the low-yield plants, iron was reduced when the RAS passed through the anaerobic and reducing conditions of the IBR, and this led to more EPS solubilization and degradation...
March 16, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28327080/effect-of-inorganic-carbon-concentration-on-the-stability-and-nitrite-oxidizing-bacteria-community-structure-of-canon-process-in-an-membrane-bioreactor
#9
Xiaojing Zhang, Yue Zhou, Yongpeng Ma, Nan Zhang, Siyu Zhao, Rongrong Zhang, Jun Zhang, Hongzhong Zhang
In completely autotrophic nitrogen removal over nitrite (CANON) process, the nitrite-oxidizing bacteria (NOB) should be effectively suppressed or thoroughly washed out. In this study, the nitrate production and the structure of NOB community under different inorganic carbon (IC) concentrations were investigated using DGGE in an membrane bioreactor (MBR). Results showed that IC decrease correspondingly lowered the nitrogen removal, and simultaneously induced the nitrate production by NOB. DGGE results indicated the IC deficit led to the biodiversity increasing of both Nitrobacter-like NOB and Nitrospira-like NOB...
March 22, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28326344/osteogenic-stem-cell-selection-for-repair-and-regeneration
#10
Marcus Tillotson, Niall Logan, Peter Brett
The first osteogenic cells to attach to a titanium (Ti) implant after placement are the multipotent stromal cells (MSCs) that circulate in the bloodstream and are recruited to the site of tissue damage. The reservoirs of these cells are heterogeneous in nature, consisting of a mixture of cells with varying differentiation abilities. In order to utilise these cells and to reduce the chance of unwanted events during regenerative therapies, the selection of a subset of cells that is truly multipotent is required...
December 2016: Bone Reports
https://www.readbyqxmd.com/read/28325705/biochemical-hydrological-and-mechanical-behaviors-of-high-food-waste-content-msw-landfill-preliminary-findings-from-a-large-scale-experiment
#11
Liang-Tong Zhan, Hui Xu, Yun-Min Chen, Fan Lü, Ji-Wu Lan, Li-Min Shao, Wei-An Lin, Pin-Jing He
A large-scale bioreactor experiment lasting for 2years was presented in this paper to investigate the biochemical, hydrological and mechanical behaviors of high food waste content (HFWC) MSW. The experimental cell was 5m in length, 5m in width and 7.5m in depth, filled with unprocessed HFWC-MSWs of 91.3 tons. In the experiment, a surcharge loading of 33.4kPa was applied on waste surface, mature leachate refilling and warm leachate recirculation were performed to improve the degradation process. In this paper, the measurements of leachate quantity, leachate level, leachate biochemistry, gas composition, waste temperature, earth pressure and waste settlement were presented, and the following observations were made: (1) 26...
March 18, 2017: Waste Management
https://www.readbyqxmd.com/read/28325660/production-of-bioethanol-from-napier-grass-via-simultaneous-saccharification-and-co-fermentation-in-a-modified-bioreactor
#12
Yu-Kuo Liu, Wei-Chuan Chen, Yu-Ching Huang, Yu-Kaung Chang, I-Ming Chu, Shen-Long Tsai, Yu-Hong Wei
The aim of this study was to use a modified bioreactor system for simultaneous saccharification of cellulose and bioethanol production. We tested Aspergillus niger and Trichoderma reesei for cellulose saccharification and Zymomonas mobilis for bioethanol production simultaneously in this modified bioreactor. The results showed that various carboxymethylcellulose (CMC) concentrations (10, 15, or 20 g/L) as a substrate for A. niger and T. reesei yielded bioethanol production of 0.51, 0.78, and 0.89 g/L and a CMC conversion rate of 10...
March 18, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28324827/anaerobic-treatment-of-hydrothermally-solubilised-sugarcane-bagasse-and-its-kinetic-modelling
#13
Bing Liu, Van Anh Ngo, Mitsuharu Terashima, Hidenari Yasui
The aim of this study was the evaluation of anaerobic treatment for the soluble organics generated from a steam-explosion pre-treatment of sugarcane bagasse. The batch analysis revealed that about 50% of the organics was possible to be degraded into methane whilst the rest was biologically inert and composed of mostly lignin. Based on the experiment a kinetic model composed of 14 kinds of soluble substances and 5 kinds of anaerobic microorganisms was developed. The model was used to simulate the process performance of a continuous anaerobic bioreactor with MLSS concentration at 2500-15,000mg/L...
March 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28324709/improvement-of-virus-removal-using-ultrafiltration-membranes-modified-with-grafted-zwitterionic-polymer-hydrogels
#14
Ruiqing Lu, Chang Zhang, Maria Piatkovsky, Mathias Ulbricht, Moshe Herzberg, Thanh H Nguyen
Potable water reuse has been adopted by cities suffering water scarcity in recent years. The microbial safety in water reuse, especially with respect to pathogenic viruses, is still a concern for water consumers. Membrane filtration can achieve sufficient removal of pathogenic viruses without disinfection byproducts, but the required energy is intensive. In this study, we graft-polymerized zwitterionic SPP ([3-(methacryloylamino) propyl] dimethyl (3-sulfopropyl) ammonium hydroxide) on a 150 kDa ultrafiltration polyethersulfone membrane to achieve a significantly higher virus removal...
March 9, 2017: Water Research
https://www.readbyqxmd.com/read/28322954/characterization-of-high-yield-exopolysaccharide-produced-by-phyllobacterium-sp-921f-exhibiting-moisture-preserving-properties
#15
Yinping Li, Gaoli Zhang, Chunying Du, Haijin Mou, Jiefen Cui, Huashi Guan, Hueymin Hwang, Peng Wang
A new strain bacteria was isolated and named as Phyllobacterium sp. 921F, due to its high production capacity of exopolysaccharide (EPS). Characterization of physico-chemical properties of the EPS and optimization for high production were conducted to aim at industrial applications. The optimum pH and temperature were 7.0 and 30°C, respectively. The following scale-up fermentation was carried out in 30L bioreactor and amounts of EPS (21.9g/L) were produced. The EPS with a molecular mass of 1082kDa was composed of glucose, galactose, and pyruvate...
March 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28322056/preparation-of-ultrathin-robust-nanohybrid-capsules-through-a-beyond-biomineralization-method
#16
Shaohua Zhang, Zhongyi Jiang, Weilun Qian, Jiafu Shi, Xiaoli Wang, Lei Tang, Hongjian Zou, Hua Liu
Herein, a facile and generic method is developed to prepare ultrathin, robust nanohybrid capsules by manipulating the dynamic structure of supramolecular nanocoatings on CaCO3 sacrificial templates through incorporating multivalent-anion substitution process into biomineralization. Above biomineralization level, multivalent anions, e.g., phosphate, sulfate or citrate, are employed to initiate the assembly of polyamine into continuous (non-segregated) polyamine-anion supramolecular nanocoatings on CaCO3 sacrificial templates...
March 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28321412/recombinant-lactococcus-lactis-expressing-haemagglutinin-from-a-polish-avian-h5n1-isolate-and-its-immunological-effect-in-preliminary-animal-trials
#17
Agnieszka K Szczepankowska, Katarzyna Szatraj, Przemysław Sałański, Agnieszka Rózga, Roman K Górecki, Jacek K Bardowski
Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and examining its effect on animals...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28319768/quantitative-evaluation-of-the-interfacial-interactions-between-a-randomly-rough-sludge-floc-and-membrane-surface-in-a-membrane-bioreactor-based-on-fractal-geometry
#18
Meijia Zhang, Xiaoling Zhou, Liguo Shen, Xiang Cai, Fangyuan Wang, Jianrong Chen, Hongjun Lin, Renjie Li, Xilin Wu, Bao-Qiang Liao
In this paper, a new method for quantification of interfacial interactions between a randomly rough particle and membrane surface was proposed. It was found that sludge flocs in a membrane bioreactor were of apparent fractal characteristics, and could be modeled by the modified two-variable Weierstrass-Mandelbrot (WM) function. By combining the surface element integration (SEI) method, differential geometry and composite Simpson's rule, the quantitation method for calculating such interfacial interactions was further developed...
March 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319761/dynamic-changes-of-dissolved-organic-matter-in-membrane-bioreactors-at-different-organic-loading-rates-evidence-from-spectroscopic-and-chromatographic-methods
#19
Tahir Maqbool, Jinwoo Cho, Jin Hur
Excitation emission matrix-parallel factor analysis (EEM-PARAFAC) and size exclusion chromatography (SEC) were utilized to explore the dynamics in extracellular polymeric substances (EPS), soluble microbial products (SMP), and effluent for the membrane bioreactors at two different organic loading rates (OLRs). Combination of three different fluorescent components explained the compositional changes of dissolved organic matter. The lower OLR resulted in a higher production of tryptophan-like component (C1) in EPS, while the opposite trends were found for the other two components (humic-like C2 and tyrosine-like C3), signifying the role of C1 in the endogenous condition...
March 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319758/trace-metals-supplementation-in-anaerobic-membrane-bioreactors-treating-highly-saline-phenolic-wastewater
#20
Julian David Muñoz Sierra, Carlos Lafita, Carmen Gabaldón, Henri Spanjers, Jules B van Lier
Biomass requires trace metals (TM) for maintaining its growth and activity. This study aimed to determine the effect of TM supplementation and partitioning on the specific methanogenic activity (SMA), with a focus on cobalt and tungsten, during the start-up of two lab-scale Anaerobic Membrane Bioreactors (AnMBRs) treating saline phenolic wastewater. The TM partitioning revealed a strong accumulation of sodium in the biomass matrix and a wash-out of the majority of TM in the reactors, which led to an SMA decrease and a low COD removal of about 30%...
March 7, 2017: Bioresource Technology
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