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Laam Li, Sébastien P Faucher
The water-borne pathogen Legionella pneumophila (Lp) strongly expresses the lpg1659 gene in water. This gene encodes a hypothetical protein predicted to be a membrane protein using in silico analysis. While no conserved domains were identified in Lpg1659, similar proteins are found in many Legionella species and other aquatic bacteria. RT-qPCR showed that lpg1659 is positively regulated by the alternative sigma factor RpoS, which is essential for Lp to survive in water. These observations suggest an important role of this novel protein in the survival of Lp in water...
2016: Frontiers in Cellular and Infection Microbiology
W Bradford DeLong, Nayak L Polissar, Moni B Neradilek, Leslie A Laam
No abstract text is available yet for this article.
September 2016: World Neurosurgery
Kang Jin, Iek Hou Sam, Kathy Hiu Laam Po, Du'an Lin, Ebrahim H Ghazvini Zadeh, Sheng Chen, Yu Yuan, Xuechen Li
To cope with the global bacterial multidrug resistance, scientific communities have devoted significant efforts to develop novel antibiotics, particularly those with new modes of actions. Teixobactin, recently isolated from uncultured bacteria, is considered as a promising first-in-class drug candidate for clinical development. Herein, we report its total synthesis by a highly convergent Ser ligation approach and this strategy allows us to prepare several analogues of the natural product.
2016: Nature Communications
Suzanne Nielsen, Briony Larance, Louisa Degenhardt, Linda Gowing, Chyanne Kehler, Nicholas Lintzeris
BACKGROUND: There are increasing concerns regarding pharmaceutical opioid harms including overdose and dependence, with an associated increase in treatment demand. People dependent on pharmaceutical opioids appear to differ in important ways from people who use heroin, yet most opioid agonist treatment research has been conducted in people who use heroin. OBJECTIVES: To assess the effects of maintenance agonist pharmacotherapy for the treatment of pharmaceutical opioid dependence...
May 9, 2016: Cochrane Database of Systematic Reviews
Jennifer R Tanner, Laam Li, Sébastien P Faucher, Ann Karen C Brassinga
The bacterium Legionella pneumophila is capable of intracellular replication within freshwater protozoa as well as human macrophages, the latter of which results in the serious pneumonia Legionnaires' disease. A primary factor involved in these host cell interactions is the Dot/Icm Type IV secretion system responsible for translocating effector proteins needed to establish and maintain the bacterial replicative niche. Several regulatory factors have been identified to control the expression of the Dot/Icm system and effectors, one of which is the CpxRA two-component system, suggesting essentiality for virulence...
June 2016: Molecular Microbiology
Kathy Hiu Laam Po, Edward Wai Chi Chan, Sheng Chen
This study assessed the functional importance of residues located at the i(-2) position of face 4 of the tandem repeat loops of the quinolone resistance protein QnrVC7 through mutagenesis studies. The i(-2) position of face 4 on different coils required residues with different natures. Some substitutions reduced the protective activity of QnrVC7, while some of them increased it. These findings advanced our understanding on the detailed structural organization and functional requirements of Qnr proteins.
March 2016: Antimicrobial Agents and Chemotherapy
Laam Li, Nilmini Mendis, Hana Trigui, Sébastien P Faucher
BACKGROUND: Legionella pneumophila (Lp) is a water-borne opportunistic pathogen. In water, Lp can survive for an extended period of time until it encounters a permissive host. Therefore, identifying genes that are required for survival in water may help develop strategies to prevent Legionella outbreaks. RESULTS: We compared the global transcriptomic response of Lp grown in a rich medium to that of Lp exposed to an artificial freshwater medium (Fraquil) for 2, 6 and 24 hours...
2015: BMC Genomics
Manuel Grana, Darya Chyzhyk
Multivariate mathematical morphology (MMM) aims to extend the mathematical morphology from gray scale images to images whose pixels are high-dimensional vectors, such as remote sensing hyperspectral images and functional magnetic resonance images (fMRIs). Defining an ordering over the multidimensional image data space is a fundamental issue MMM, to ensure that ensuing morphological operators and filters are mathematically consistent. Recent approaches use the outputs of two-class classifiers to build such reduced orderings...
September 2016: IEEE Transactions on Neural Networks and Learning Systems
Brittany B Dennis, Monica Bawor, James Paul, Michael Varenbut, Jeff Daiter, Carolyn Plater, Guillaume Pare, David C Marsh, Andrew Worster, Dipika Desai, Lehana Thabane, Zainab Samaan
BACKGROUND: The consequences of opioid relapse among patients being treated with opioid substitution treatment (OST) are serious and can result in abnormal cardiovascular function, overdose, and mortality. Chronic pain is a major risk factor for opioid relapse within the addiction treatment setting. There exist a number of opioid maintenance therapies including methadone, buprenorphine, naltrexone, and levomethadyl acetate (LAAM), of which the mediating effects of pain on treatment attrition, substance use behavior, and social functioning may differ across therapies...
2015: Systematic Reviews
Darya Chyzhyk, Manuel Graña, Döst Öngür, Ann K Shinn
Auditory hallucinations (AH) are a symptom that is most often associated with schizophrenia, but patients with other neuropsychiatric conditions, and even a small percentage of healthy individuals, may also experience AH. Elucidating the neural mechanisms underlying AH in schizophrenia may offer insight into the pathophysiology associated with AH more broadly across multiple neuropsychiatric disease conditions. In this paper, we address the problem of classifying schizophrenia patients with and without a history of AH, and healthy control (HC) subjects...
May 2015: International Journal of Neural Systems
Yeow Kuan Chong, Chloe Miu Mak, Hiu Laam Lam, Man Han Lau, Daniel Cheuk Wa Leung
BACKGROUND: It is well known that enzymatic assays for acetaminophen are positively interfered by bilirubin. The effect on acetaminophen not only depends on the concentration of bilirubin but also on that of acetaminophen. We demonstrated a negative interference instead of a positive one in a commonly used routine analyzer and investigated the recovery of acetaminophen in an enzymatic assay by a bi-variate regression. METHODS: Commercially available blank serum specimens were spiked with acetaminophen and bilirubin at various concentrations, and were analyzed in the Beckman Coulter AU5822 analyzer...
February 2015: Clinical Biochemistry
Carmen K M Wong, Jun Liang, Man L Chan, Yin H Chan, Laam Chan, Kwong Y Wan, Ming S Ng, Dicken C C Chan, Samuel Y S Wong, Martin C S Wong
OBJECTIVE: Depression is common in women with much research focusing on hormonal changes and menopausal symptoms but with little exploration of psychosocial problems in midlife. This study investigates the prevalence of clinically relevant depressive symptoms in midlife Chinese women and its association with psychosocial factors. METHODS: A cross-sectional, community-based household survey of women aged 45 to 64 years of age was conducted in Hong Kong from September 2010 to March 2011...
2014: PloS One
David S Maxwell, Duoli Sun, Zhenghong Peng, Diana V Martin, Basvoju A Bhanu Prasad, William G Bornmann
We report the synthesis of a macrocycle utilizing a novel framework of standard amino acids in combination with subunits that we have named as Linked Amino Acid Mimetics (LAAM's). Macrocycles based on the LAAM concept provide both a peptide targeting region and two independently variable functional regions. In the prototype structure, the commonly known Arg-Gly-Asp (RGD) sequence was used for the targeting region. The functional regions contain a phenyl group, and the linkage was formed via a Ring-Closing Metathesis (RCM) reaction...
October 23, 2013: Tetrahedron Letters
Silvia Minozzi, Laura Amato, Cristina Bellisario, Marina Davoli
BACKGROUND: The scientific literature examining effective treatments for opioid-dependent adults clearly indicates that pharmacotherapy is a necessary and acceptable component. Nevertheless, no reviews have been published that systematically assess the effectiveness of pharmacological maintenance treatment in adolescents. OBJECTIVES: To assess the effectiveness of any maintenance treatment alone or in combination with psychosocial intervention compared to no intervention, other pharmacological intervention or psychosocial interventions for retaining adolescents in treatment, reducing the use of substances and improving health and social status...
2014: Cochrane Database of Systematic Reviews
Kiran Kumar Solingapuram Sai, Jinda Fan, Zhude Tu, Patrick Zerkel, Robert H Mach, Evan D Kharasch
Long-acting opioid agonists methadone and l-α-acetylmethadol (LAAM) prevent withdrawal in opioid-dependent persons. Attempts to synthesize [(11)C]-methadone for PET evaluation of brain disposition were unsuccessful. Owing, however, to structural and pharmacologic similarities, we aimed to develop [(11)C]LAAM as a PET ligand to probe the brain exposure of long-lasting opioids in humans. This manuscript describes [(11)C]LAAM synthesis and its biodistribution in mice. The radiochemical synthetic strategy afforded high radiochemical yield, purity and specific activity, thereby making the synthesis adaptable to automated modules...
September 2014: Applied Radiation and Isotopes
Laam Li, Nilmini Mendis, Hana Trigui, James D Oliver, Sebastien P Faucher
Many bacterial species have been found to exist in a viable but non-culturable (VBNC) state since its discovery in 1982. VBNC cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques. This leads to an underestimation of total viable cells in environmental or clinical samples, and thus poses a risk to public health. In this review, we present recent findings on the VBNC state of human bacterial pathogens. The characteristics of VBNC cells, including the similarities and differences to viable, culturable cells and dead cells, and different detection methods are discussed...
2014: Frontiers in Microbiology
Zahra Zamani, Nassir-Ud-Din, Haleemeh Kabini Kohan, Mehdi Kadivar, Zahra Kalyee, Behzad Laame Rad, Ayda Iravani, Nourooz Ali Rahimi, Farideh Wahabi, Sedigheh Sadeghi, Fatemeh Pourfallah, Mohammad Arjmand
Protein glycosylation is associated with the development and progression of specific diseases, including cancers. The ginger rhizome is known to have anti-cancer and anti-fungal properties. This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells. A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C. Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software...
January 15, 2014: Pakistan Journal of Biological Sciences: PJBS
Kathryn L Hamilton, Andrew C Harris, Jonathan C Gewirtz
Although the long-acting opiate methadone is commonly used to treat drug addiction, relatively little is known about the effects of withdrawal from this drug in preclinical models. The current study examined affective, neuroendocrine, and somatic signs of withdrawal from the longer-acting methadone derivative l-alpha-acetylmethydol (LAAM) in rats. Anxiety-like behavior during both spontaneous and antagonist-precipitated withdrawal was measured by potentiation of the startle reflex. Withdrawal elevated corticosterone and somatic signs and blunted circadian variations in baseline startle responding...
November 13, 2013: Brain Research
Emily H M Wong, Chun-Laam Ng, Vincent Chi-Hang Lui, Man-ting So, Stacey S Cherny, Pak-Chung Sham, Paul Kwong-Hang Tam, Maria-Mercè Garcia-Barceló
Anorectal malformations (ARMs) are birth defects that require surgery and carry significant chronic morbidity. Our earlier genome-wide copy number variation (CNV) study had provided a wealth of candidate loci. To find out whether these candidate loci are related to important developmental pathways, we have performed an extensive literature search coupled with the currently available bioinformatics tools. This has allowed us to assign both genic and non-genic CNVs to interrelated pathways known to govern the development of the anorectal region...
2013: PloS One
Hana Trigui, Nilmini Mendis, Laam Li, Mariam Saad, Sebastien P Faucher
Legionella pneumophila is a water-borne pathogen that causes a severe lung infection in humans. It is able to replicate inside amoeba in the water environment, and inside lung macrophages in humans. Efficient regulation of gene expression is critical for responding to the conditions that L. pneumophila encounters and for intracellular multiplication in host cells. In the last two decades, many reports have contributed to our understanding of the critical importance of small regulatory RNAs (sRNAs) in the regulatory network of bacterial species...
2013: Current Topics in Microbiology and Immunology
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