keyword
MENU ▼
Read by QxMD icon Read
search

Proteomics and blood biomarkers

keyword
https://www.readbyqxmd.com/read/29466886/proteomic-approaches-to-identify-blood-based-biomarkers-for-depression-and-bipolar-disorders
#1
Rhian Lauren Preece, Sung Yeon Sarah Han, Sabine Bahn
Major depressive disorder (MDD) and bipolar disorder (BD) are leading causes of disability worldwide, yet many people remain undiagnosed, are misdiagnosed, and/or ineffectively treated. Diagnosis relies on the clinical assessment of symptoms, and there is currently no molecular or brain-imaging diagnostic test available. Identifying and validating protein biomarkers could provide a more accurate and objective means of diagnosis. Areas covered: Proteomics is a powerful tool that enables the identification and quantification of novel candidate biomarkers of disease...
February 22, 2018: Expert Review of Proteomics
https://www.readbyqxmd.com/read/29462970/approaches-for-reducing-the-risk-of-early-life-iron-deficiency-induced-brain-dysfunction-in-children
#2
REVIEW
Sarah E Cusick, Michael K Georgieff, Raghavendra Rao
Iron deficiency is the most common micronutrient deficiency in the world. Women of reproductive age and young children are particularly vulnerable. Iron deficiency in late prenatal and early postnatal periods can lead to long-term neurobehavioral deficits, despite iron treatment. This may occur because screening and treatment of iron deficiency in children is currently focused on detection of anemia and not neurodevelopment. Anemia is the end-stage state of iron deficiency. The brain becomes iron deficient before the onset of anemia due to prioritization of the available iron to the red blood cells (RBCs) over other organs...
February 17, 2018: Nutrients
https://www.readbyqxmd.com/read/29451788/a-systematic-evaluation-of-the-use-of-human-plasma-and-serum-for-mass-spectrometry-based-shotgun-proteomics
#3
Jiayi Lan, Antonio Núñez Galindo, James Doecke, Christopher Fowler, Ralph N Martins, Stephanie R Rainey-Smith, Ornella Cominetti, Loïc Dayon
Over the last two decades, EDTA-plasma has been used as the preferred sample matrix for human blood proteomic profiling. Serum has also been employed widely. Only a few studies have assessed the difference and relevance of the proteome profiles obtained from plasma samples, such as EDTA-plasma or lithium-heparin-plasma, and serum. A more complete evaluation of the use of EDTA-plasma, heparin-plasma, and serum would greatly expand the comprehensiveness of shotgun proteomics of blood samples. In this study, we evaluated the use of heparin-plasma with respect to EDTA-plasma and serum to profile blood proteomes using a scalable automated proteomic pipeline (ASAP2)...
February 16, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29449599/red-blood-cells-are-dynamic-reservoirs-of-cytokines
#4
Elisabeth Karsten, Edmond Breen, Benjamin R Herbert
Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex ® technology...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29448998/cardiac-amyloidosis-from-clinical-suspicion-to-morphological-diagnosis
#5
REVIEW
Pavla Flodrova, Patrik Flodr, Tomas Pika, Jiri Vymetal, Dusan Holub, Petr Dzubak, Marian Hajduch, Vlastimil Scudla
Amyloidosis is a heterogeneous group of diseases characterised by extracellular accumulation of amyloid in various tissues and organs of the body, leading to alteration and destruction of tissues. Heart involvement is the most important prognostic factor in patients with systemic amyloidosis and the diagnosis and typing of amyloid must be made properly. The clinical picture shows congestive heart failure with predominant right-sided heart failure symptoms in fully developed disease, various types of arrhythmias and characteristic electrocardiography and echocardiography findings...
February 12, 2018: Pathology
https://www.readbyqxmd.com/read/29441425/detailed-analysis-of-the-plasma-extracellular-vesicle-proteome-after-separation-from-lipoproteins
#6
Nasibeh Karimi, Aleksander Cvjetkovic, Su Chul Jang, Rossella Crescitelli, Mohammad Ali Hosseinpour Feizi, Rienk Nieuwland, Jan Lötvall, Cecilia Lässer
The isolation of extracellular vesicles (EVs) from blood is of great importance to understand the biological role of circulating EVs and to develop EVs as biomarkers of disease. Due to the concurrent presence of lipoprotein particles, however, blood is one of the most difficult body fluids to isolate EVs from. The aim of this study was to develop a robust method to isolate and characterise EVs from blood with minimal contamination by plasma proteins and lipoprotein particles. Plasma and serum were collected from healthy subjects, and EVs were isolated by size-exclusion chromatography (SEC), with most particles being present in fractions 8-12, while the bulk of the plasma proteins was present in fractions 11-28...
February 13, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29432918/exploration-of-the-platelet-proteome-in-patients-with-early-stage-cancer
#7
Siamack Sabrkhany, Marijke J E Kuijpers, Jaco C Knol, Steven W M Olde Damink, Anne-Marie C Dingemans, Henk M Verheul, Sander R Piersma, Thang V Pham, Arjan W Griffioen, Mirjam G A Oude Egbrink, Connie R Jimenez
Platelets play an important role in tumor growth and, at the same time, platelet characteristics are affected by cancer presence. Therefore, we investigated whether the platelet proteome harbors differentially expressed proteins associated with early-stage cancer. For this proof-of-concept study, patients with early-stage lung (n = 8) or head of pancreas cancer (n = 4) were included, as were healthy sex- and age-matched controls for both subgroups. Blood samples were collected from controls and from patients before surgery...
February 9, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29427919/the-building-blocks-of-successful-translation-of-proteomics-to-the-clinic
#8
REVIEW
Paul Kearney, J Jay Boniface, Nathan D Price, Leroy Hood
Recently, the first two multiplexed tests using selective reaction monitoring (SRM-MS) mass spectrometry have entered clinical practice. Despite different areas of indication, risk stratification in lung cancer and preterm birth, they share multiple steps in their development strategies. Here we review these strategies and their implications for successful translation of biomarkers to clinical practice. We believe that the identification of blood protein panels for the identification of disease phenotypes is now a reproducible and standard (albeit complex) process...
February 6, 2018: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/29425228/kshv-oral-shedding-and-plasma-viremia-result-in-significant-changes-in-the-extracellular-tumorigenic-mirna-expression-profile-in-individuals-infected-with-the-malaria-parasite
#9
Minako Ikoma, Soren Gantt, Corey Casper, Yuko Ogata, Qing Zhang, Ryan Basom, Michael R Dyen, Timothy M Rose, Serge Barcy
Kaposi's sarcoma herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma (KS). Both KSHV and HIV infections are endemic in Uganda, where KS is among the most common cancers in HIV-infected individuals. Recent studies examined the use of small RNAs as biomarkers of disease, including microRNAs (miRNAs), with viral and tumor-derived miRNAs being detected in exosomes from individuals with KSHV-associated malignancies. In the current study, the host and viral extracellular mature miRNA expression profiles were analyzed in blood of KS-negative individuals in Uganda, comparing those with or without KSHV detectable from the oropharynx...
2018: PloS One
https://www.readbyqxmd.com/read/29414888/mass-spectrometry-based-proteomic-profiling-of-thrombotic-material-obtained-by-endovascular-thrombectomy-in-patients-with-ischemic-stroke
#10
Roberto Muñoz, Enrique Santamaría, Idoya Rubio, Karina Ausín, Aiora Ostolaza, Alberto Labarga, Miren Roldán, Beatriz Zandio, Sergio Mayor, Rebeca Bermejo, Mónica Mendigaña, María Herrera, Nuria Aymerich, Jorge Olier, Jaime Gállego, Maite Mendioroz, Joaquín Fernández-Irigoyen
Thrombotic material retrieved from acute ischemic stroke (AIS) patients represents a valuable source of biological information. In this study, we have developed a clinical proteomics workflow to characterize the protein cargo of thrombi derived from AIS patients. To analyze the thrombus proteome in a large-scale format, we developed a workflow that combines the isolation of thrombus by endovascular thrombectomy and peptide chromatographic fractionation coupled to mass-spectrometry. Using this workflow, we have characterized a specific proteomic expression profile derived from four AIS patients included in this study...
February 7, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29413716/proteomic-profiling-of-epileptogenesis-in-a-rat-model-focus-on-cell-stress-extracellular-matrix-and-angiogenesis
#11
Michael Keck, Roelof Maarten van Dijk, Cornelia A Deeg, Katharina Kistler, Andreas Walker, Eva-Lotta von Rüden, Vera Russmann, Stefanie M Hauck, Heidrun Potschka
Information about epileptogenesis-associated changes in protein expression patterns is of particular interest for future selection of target and biomarker candidates. Bioinformatic analysis of proteomic data sets can increase our knowledge about molecular alterations characterizing the different phases of epilepsy development following an initial epileptogenic insult. Here, we report findings from a focused analysis of proteomic data obtained for the hippocampus and parahippocampal cortex samples collected during the early post-insult phase, latency phase, and chronic phase of a rat model of epileptogenesis...
April 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29412517/plasma-proteomic-signatures-in-early-chronic-obstructive-pulmonary-disease
#12
Angela Baralla, Alessandro G Fois, Elisabetta Sotgiu, Elisabetta Zinellu, Arduino A Mangoni, Salvatore Sotgia, Angelo Zinellu, Pietro Pirina, Ciriaco Carru
PURPOSE: Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation and abnormal inflammatory response of the lungs to inhaled noxious particles or gases. We used a proteomic approach with 2-DE followed by MALDI TOF-MS analyses in order to identify potential biomarkers in the early stages of the disease: GOLD stage mild and moderate. EXPERIMENTAL DESIGN: Blood plasma was collected from 43 patients with mild and moderate COPD as well as from 43 age and sex matched control subjects...
February 7, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29385837/verification-of-a-proteomic-biomarker-panel-to-diagnose-minor-stroke-and-transient-ischaemic-attack-phase-1-of-spectra-a-large-scale-translational-study
#13
Andrew M Penn, Maximilian B Bibok, Viera K Saly, Shelagh B Coutts, Mary L Lesperance, Robert F Balshaw, Kristine Votova, Nicole S Croteau, Anurag Trivedi, Angela M Jackson, Janka Hegedus, Evgenia Klourfeld, Amy Y X Yu, Charlotte Zerna, Christoph H Borchers
OBJECTIVE: To derive a plasma biomarker protein panel from a list of 141 candidate proteins which can differentiate transient ischaemic attack (TIA)/minor stroke from non-cerebrovascular ('mimic') conditions in emergency department (ED) settings. DESIGN: Prospective clinical study (#NCT03050099) with up to three timed blood draws no more than 36 hours following symptom onset. Plasma samples analysed by multiple reaction monitoring-mass spectrometry (MRM-MS). PARTICIPANTS: 545 participants suspected of TIA enrolled in the EDs of two urban medical centres...
February 1, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/29382491/omics-techniques-and-biobanks-to-find-new-biomarkers-for-the-early-detection-of-acute-lymphoblastic-leukemia-in-middle-income-countries-a-perspective-from-mexico
#14
REVIEW
William Alejandro Aguirre-Guillén, Tania Angeles-Floriano, Briceida López-Martínez, Hortensia Reyes-Morales, Albert Zlotnik, Ricardo Valle-Rios
Acute lymphoblastic leukemia (ALL) affects the quality of life of many children in the world and particularly in Mexico, where a high incidence has been reported. With a proper financial investment and with well-organized institutions caring for those patients, together with solid platforms to perform high-throughput analyses, we propose the creation of a Mexican repository system of serum and cells from bone marrow and blood samples derived from tissues of pediatric patients with ALL diagnosis. This resource, in combination with omics technologies, particularly proteomics and metabolomics, would allow longitudinal studies, offering an opportunity to design and apply personalized ALL treatments...
May 2017: Boletín Médico del Hospital Infantil de México
https://www.readbyqxmd.com/read/29380307/proteomic-studies-of-psychiatric-disorders
#15
Paul C Guest
Many diseases result from programming effects in utero. This chapter describes recent advances in proteomic studies which have improved our understanding of the underlying pathophysiological pathways in the major psychiatric disorders, resulting in the development of potential novel biomarker tests. Such tests should be based on measurement of blood-based proteins given the ease of accessibility of this medium and the known connections between the periphery and the central nervous system. Most importantly, emerging biomarker tests should be developed on lab-on-a-chip and other handheld devices to enable point-of-care use...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29378352/atherothrombosis-and-thromboembolism-position-paper-from-the-second-maastricht-consensus-conference-on-thrombosis
#16
H M H Spronk, T Padro, J E Siland, J H Prochaska, J Winters, A C van der Wal, J J Posthuma, G Lowe, E d'Alessandro, P Wenzel, D M Coenen, P H Reitsma, W Ruf, R H van Gorp, R R Koenen, T Vajen, N A Alshaikh, A S Wolberg, F L Macrae, N Asquith, J Heemskerk, A Heinzmann, M Moorlag, N Mackman, P van der Meijden, J C M Meijers, M Heestermans, T Renné, S Dólleman, W Chayouâ, R A S Ariëns, C C Baaten, M Nagy, A Kuliopulos, J J Posma, P Harrison, M J Vries, H J G M Crijns, E A M P Dudink, H R Buller, Y M C Henskens, A Själander, S Zwaveling, O Erküner, J W Eikelboom, A Gulpen, F E C M Peeters, J Douxfils, R H Olie, T Baglin, A Leader, U Schotten, B Scaf, H M M van Beusekom, L O Mosnier, L van der Vorm, P Declerck, M Visser, D W J Dippel, V J Strijbis, K Pertiwi, A J Ten Cate-Hoek, H Ten Cate
Atherothrombosis is a leading cause of cardiovascular mortality and long-term morbidity. Platelets and coagulation proteases, interacting with circulating cells and in different vascular beds, modify several complex pathologies including atherosclerosis. In the second Maastricht Consensus Conference on Thrombosis, this theme was addressed by diverse scientists from bench to bedside. All presentations were discussed with audience members and the results of these discussions were incorporated in the final document that presents a state-of-the-art reflection of expert opinions and consensus recommendations regarding the following five topics: 1...
February 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29368175/differential-proteomics-for-distinguishing-ischemic-stroke-from-controls-a-pilot-study-of-the-spectra-project
#17
A M Penn, V Saly, A Trivedi, M L Lesperance, K Votova, A M Jackson, N S Croteau, R F Balshaw, M B Bibok, D S Smith, K K Lam, J Morrison, L Lu, S B Coutts, C H Borchers
A diagnostic blood test for stroke is desirable but will likely require multiple proteins rather than a single "troponin." Validating large protein panels requires large patient numbers. Mass spectrometry (MS) is a cost-effective tool for this task. We compared differences in the abundance of 147 protein markers to distinguish 20 acute cerebrovascular syndrome (ACVS) patients who presented to the Emergency Department of one urban hospital within < 24 h from onset) and from 20 control patients who were enrolled via an outpatient neurology clinic...
January 24, 2018: Translational Stroke Research
https://www.readbyqxmd.com/read/29362827/calorimetric-markers-for-monitoring-of-multiple-myeloma-and-waldenstr%C3%A3-m-s-macroglobulinemia-patients
#18
Svetla Todinova, Sashka Krumova, Avgustina Danailova, Violeta Petkova, Margarita Guenova, Georgi Mihaylov, Lidia Gartcheva, Stefka Germanova Taneva
The blood proteome has been studied extensively for identification of novel reliable disease biomarkers. In recent years, differential scanning calorimetry has emerged as a new tool for characterization of the thermodynamic properties of the major serum/plasma proteins and for the establishment of calorimetric markers for a variety of diseases. Here we applied calorimetry to monitor the effect of treatment of patients diagnosed with multiple myeloma and Waldenström's macroglobulinemia on the calorimetric profiles of patients' blood sera...
January 23, 2018: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/29353230/a-model-of-dormant-emergent-metastatic-breast-cancer-progression-enabling-exploration-of-biomarker-signatures
#19
Amanda M Clark, Manu Kumar, Sarah Wheeler, Carissa Young, Raman Venkataramanan, Donna Stolz, Linda Griffith, Douglas A Lauffenburger, Alan Wells
Breast cancer mortality predominantly results from dormant micrometastases that emerge as fatal outgrowths years after initial diagnosis. In order to gain insights concerning factors associated with emergence of liver metastases, we recreated spontaneous dormancy in an all-human ex vivo hepatic microphysiological system (MPS). Seeding this MPS with small numbers (<0.05% by cell count) of the aggressive MDA-MB-231 breast cancer cell line, two populations formed: actively proliferating ('growing'; EdU+), and spontaneously quiescent ('dormant'; EdU-)...
January 20, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29348854/acid-ceramidase-and-its-inhibitors-a-de-novo-drug-target-and-a-new-class-of-drugs-for-killing-glioblastoma-cancer-stem-cells-with-high-efficiency
#20
Ninh B Doan, Hisham Alhajala, Mona M Al-Gizawiy, Wade M Mueller, Scott D Rand, Jennifer M Connelly, Elizabeth J Cochran, Christopher R Chitambar, Paul Clark, John Kuo, Kathleen M Schmainda, Shama P Mirza
Glioblastoma remains the most common, malignant primary cancer of the central nervous system with a low life expectancy and an overall survival of less than 1.5 years. The treatment options are limited and there is no cure. Moreover, almost all patients develop recurrent tumors, which typically are more aggressive. Therapeutically resistant glioblastoma or glioblastoma stem-like cells (GSCs) are hypothesized to cause this inevitable recurrence. Identifying prognostic biomarkers of glioblastoma will potentially advance knowledge about glioblastoma tumorigenesis and enable discovery of more effective therapies...
December 22, 2017: Oncotarget
keyword
keyword
52277
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"