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Intracellular calcium

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https://www.readbyqxmd.com/read/28641207/ros-and-calcium-signaling-mediated-pathways-involved-in-stress-responses-of-the-marine-microalgae-dunaliella-salina-to-enhanced-uv-b-radiation
#1
Xinxin Zhang, Xuexi Tang, Ming Wang, Wei Zhang, Bin Zhou, You Wang
UV-B ray has been addressed to trigger common metabolic responses on marine microalgae, however, the upstream events responsible for these changes in marine microalgae are poorly understood. In the present study, a species of marine green microalgae Dunaliella salina was exposed to a series of enhanced UV-B radiation ranging from 0.25 to 1.00 KJ·m(-2) per day. The role of ROS and calcium signaling in the D. salina responses to UV-B was discussed. Results showed that enhanced UV-B radiation markedly decreased the cell density in a dose-dependent manner, but the contents of protein and glycerol that were essential for cell growth increased...
May 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28640982/mechanisms-of-sphingosine-1-phosphate-mediated-vasoconstriction-of-rat-afferent-arterioles
#2
Zhengrong Guan, Fuchenchu Wang, Xiangqin Cui, Edward W Inscho
AIM: Sphingosine-1-phosphate (S1P) influences resistance vessel function and is implicated in renal pathological processes. Previous studies revealed that S1P evoked potent vasoconstriction of the preglomerular microvasculature, but the underlying mechanisms remain incompletely defined. We postulated that S1P-mediated preglomerular microvascular vasoconstriction involves activation of voltage-dependent L-type calcium channels (L-VDCC) and the rho/rho kinase pathway. METHODS: Afferent arteriolar reactivity was assessed in vitro using the blood-perfused rat juxtamedullary nephron preparation and diameter was measured during exposure to physiological and pharmacological agents...
June 22, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28640887/epidermal-growth-factor-promotes-cyclin-g2-degradation-via-calpain-mediated-proteolysis-in-gynaecological-cancer-cells
#3
Stefanie Bernaudo, Shahin Khazai, Eilyad Honarparvar, Alina Kopteva, Chun Peng
Cyclin G2 (CCNG2) is an atypical cyclin that functions to inhibit cell cycle progression and is often dysregulated in human cancers. We have previously shown that cyclin G2 is highly unstable and can be degraded through the ubiquitin/proteasome pathway. Furthermore, cyclin G2 contains a PEST domain, which has been suggested to act as a signal for degradation by multiple proteases. In this study, we determined if calpains, a family of calcium-dependent proteases, are also involved in cyclin G2 degradation. The addition of calpain inhibitors or silencing of calpain expression by siRNAs strongly enhanced cyclin G2 levels...
2017: PloS One
https://www.readbyqxmd.com/read/28636748/e-cadherin-mediates-uvr-and-calcium-induced-melanin-transfer-in-human-skin-cells
#4
Suman K Singh, Richard Baker, Stephen K Sikkink, Carine Nizard, Sylvianne Schnebert, Robin Kurfurst, Desmond J Tobin
Skin pigmentation is directed by epidermal-melanin units, characterized by long-lived and dendritic epidermal melanocytes (MC) that interact with viable keratinocytes (KC) to contribute melanin to the epidermis. Previously we reported that MC:KC contact is required for melanosome transfer, that this can be enhanced by filopodial and by UVR/UVA irradiation, which can up-regulate melanosome transfer via Myosin X-mediated control of MC filopodia. Both MC and KC express Ca(2+) -dependent E-cadherins. These homophilic adhesion contacts induce transient increases in intra-KC Ca(2+) , while ultraviolet radiation (UVR) raises intra-MC Ca(2+) via calcium selective ORAI1 ion channels; both are associated with regulating melanogenesis...
June 21, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28635397/induction-of-apoptosis-and-autophagy-by-calcifying-nanoparticles-in-human-bladder-cancer-cells
#5
Ji-Hua Wu, Yao-Liang Deng, Quan Liu, Jun-Chuan Yu, Yun-Long Liu, Zi-Qi He, Xiao-Feng Guan
Calcifying nanoparticles have been linked to various types of human disease, but how they contribute to disease processes is unclear. Here, we examined whether and how calcifying nanoparticles isolated from patients with kidney stones are cytotoxic to human bladder cancer cells. Calcifying nanoparticles were isolated from midstream urine of patients with renal calcium oxalate stones and examined by electron microscopy. Human bladder cancer cells (EJ cells) were cultured in the presence of calcifying nanoparticles or nanohydroxyapatites for 12 and 72 h and examined for toxicity using the Cell Counting Kit-8, for autophagy using transmission electron microscopy and confocal microscopy, and for apoptosis using fluorescence microscopy, transmission electron microscopy, and flow cytometry...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28635329/lead-induces-siberian-tiger-fibroblast-apoptosis-by-interfering-with-intracellular-homeostasis
#6
Zheng Liu, Hui Wang, Wenxiu Zhang, Ziao Yuan, Hongyi Yuan, Xueting Liu, Minghai Zhang, Xuesong Guo, Weijun Guan
Lead (Pb(2+)) is a poisonous heavy metal that causes many pathophysiological effects in living systems. Its toxicological effects are well known as it causes apoptosis of several cell types and tissues. This study aimed to determine the criteria required for early diagnosis of Pb(2+) poisoning in the Siberian tiger using a tiger population in China, to identify a safety Pb(2+) concentration threshold, and to provide suggestions for preventing Pb(2+) poisoning in Siberian tigers. We investigated the apoptotic effects of Pb(2+) (0, 32, 64, and 125 μM) for 12-48 h on Siberian tiger fibroblasts in vitro...
June 21, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28634074/distinct-regulation-of-activity-dependent-transcription-of-immediate-early-genes-in-cultured-rat-cortical-neurons
#7
Mamoru Fukuchi, Tomofumi Sanabe, Toshifumi Watanabe, Takane Kubota, Akiko Tabuchi, Masaaki Tsuda
The activity-regulated expression of immediate early genes (IEGs) contributes to long-lasting neuronal functions underlying long-term memory. However, their response properties following neuronal activity are unique and remain poorly understood. To address this knowledge gap, here we further investigated the response properties of two representative IEGs, c-fos and brain-derived neurotrophic factor (Bdnf). Treatment of cultured cortical cells with KCl produces a depolarization process that results in the increase of intracellular calcium concentration in a KCl concentration-dependent manner...
June 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28631947/predominant-contribution-of-l-type-cav1-2-channel-stimulation-to-impaired-intracellular-calcium-and-cerebral-artery-vasoconstriction-in-diabetic-hyperglycemia
#8
Stefano Morotti, Madeline Nieves-Cintrón, Matthew A Nystoriak, Manuel F Navedo, Eleonora Grandi
Enhanced L-type Ca(2+) channel (LTCC) activity in arterial myocytes contributes to vascular dysfunction during diabetes. Modulation of LTCC activity under hyperglycemic conditions could result from membrane potential-dependent and independent mechanisms. We have demonstrated that elevations in extracellular glucose (HG), similar to hyperglycemic conditions during diabetes, stimulate LTCC activity through phosphorylation of CaV1.2 at serine 1928. Prior studies have also shown that HG can suppress the activity of K(+) channels in arterial myocytes, which may contribute to vasoconstriction via membrane depolarization...
February 10, 2017: Channels
https://www.readbyqxmd.com/read/28631094/post-translational-remodeling-of-ryanodine-receptor-induces-calcium-leak-leading-to-alzheimer-s-disease-like-pathologies-and-cognitive-deficits
#9
Alain Lacampagne, Xiaoping Liu, Steven Reiken, Renaud Bussiere, Albano C Meli, Inger Lauritzen, Andrew F Teich, Ran Zalk, Nathalie Saint, Ottavio Arancio, Charlotte Bauer, Fabrice Duprat, Clark A Briggs, Shreaya Chakroborty, Grace E Stutzmann, Michael L Shelanski, Frederic Checler, Mounia Chami, Andrew R Marks
The mechanisms underlying ryanodine receptor (RyR) dysfunction associated with Alzheimer disease (AD) are still not well understood. Here, we show that neuronal RyR2 channels undergo post-translational remodeling (PKA phosphorylation, oxidation, and nitrosylation) in brains of AD patients, and in two murine models of AD (3 × Tg-AD, APP (+/-) /PS1 (+/-)). RyR2 is depleted of calstabin2 (KFBP12.6) in the channel complex, resulting in endoplasmic reticular (ER) calcium (Ca(2+)) leak. RyR-mediated ER Ca(2+) leak activates Ca(2+)-dependent signaling pathways, contributing to AD pathogenesis...
June 19, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28630064/pneumolysin-dependent-calpain-activation-and-il-1%C3%AE-secretion-in-macrophages-infected-with-streptococcus-pneumoniae
#10
Rendong Fang, Rui Wu, Huihui Du, Meilan Jin, Yajing Liu, Guihua Lei, Bing Jiang, Zehui Lei, Yuanyi Peng, Kui Nie, Kohsuke Tsuchiya
Pneumolysin (PLY), a major virulence factor of Streptococcus pneumoniae, is a pore-forming cytolysin that modulates host innate responses contributing to host defense against and pathogenesis of pneumococcal infections. Interleukin-1α (IL-1α) has been shown to be involved in tissue damage in a pneumococcal pneumonia model, however, the mechanism by which this cytokine is produced during S. pneumoniae infection remains unclear. In this study, we examined the role of PLY in IL-1α production. Although the strains induced similar levels of pro-IL-1α expression, wild-type S...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28629039/time-dependent-subcellular-structure-injuries-induced-by-nano-micron-sized-calcium-oxalate-monohydrate-and-dihydrate-crystals
#11
Xin-Yuan Sun, Kai Yu, Jian-Ming Ouyang
Comparative studies were conducted to investigate the time effect of cell injury induced by nano-sized (50nm) and micron-sized (10μm) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals in African green monkey renal epithelial (Vero) cells. The effects of nano-/micron-sized COM and COD exposure on Vero cells were investigated by detecting the cell viability, cell morphology, LDH release, reactive oxygen species, mitochondrial membrane potential, cell cycle, and cell apoptosis, as well as the intracellular and extracellular crystal distribution...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627733/response-of-renal-tubular-cells-to-differential-types-and-doses-of-calcium-oxalate-crystals-integrative-proteome-network-analysis-and-functional-investigations
#12
Arada Vinaiphat, Siripat Aluksanasuwan, Juthatip Manissorn, Suchitra Sutthimethakorn, Visith Thongboonkerd
We have previously identified changes in the cellular proteome of renal tubular cells induced by low-dose (100 μg/ml) and high-dose (1,000 μg/ml) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals. However, the functional significance of such expression data remained unclear. In this study, we performed comparative analyses and functional investigations of four proteomic datasets to define potential mechanisms by which renal tubular cells responded to differential crystal types and doses. The data showed that high-dose induced greater changes than low-dose, whereas COM induced more changes than COD...
June 19, 2017: Proteomics
https://www.readbyqxmd.com/read/28627017/functional-expression-of-calcium-permeable-canonical-transient-receptor-potential-4-containing-channels-promotes-migration-of-medulloblastoma-cells
#13
Wei-Chun Wei, Wan-Chen Huang, Yu-Ping Lin, Esther B E Becker, Olaf Ansorge, Veit Flockerzi, Daniele Conti, Giovanna Cenacchi, Maike D Glitsch
Aberrant intracellular Ca(2+) signalling contributes to the formation and progression of a range of distinct pathologies including cancers. Rises in intracellular Ca(2+) concentration occur in response to Ca(2+) influx through plasma membrane channels and Ca(2+) release from intracellular Ca(2+) stores, which can be mobilised in response to activation of cell surface receptors. OGR1 (Ovarian cancer G protein coupled Receptor 1, aka GPR68) is a proton-sensing Gq -coupled receptor that is most highly expressed in cerebellum...
June 19, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28625263/-bacterial-endotoxin-induced-endothelial-cell-injury-and-calcium-overload-associated-with-toll-like-receptor-and-calcium-signal
#14
Jianguo Xiao, Qing Song, Tanshi Li, Rongju Sun
OBJECTIVE: To explore the effect of toll-like receptor 4 (TLR4), myeloid differentiation protein-2 (MD2), and stromal interaction molecular 1 (STIM1) for regulating human vascular endothelial calcium overload injury and inflammatory reaction induced by bacterial endotoxin (LPS). METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured in Dulbecco's modification of Eagle's medium (DMEM). (1) The levels of TLR4, MD2 and nuclear factor-κB (NF-κB) were detected by reverse transcriotion-polymerase chain reaction (RT-PCR) before and 0...
February 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28624457/effect-of-calcium-carbonate-particle-shape-on-phagocytosis-and-pro-inflammatory-response-in-differentiated-thp-1-macrophages
#15
Yosuke Tabei, Sakiko Sugino, Kenichiro Eguchi, Masahiko Tajika, Hiroko Abe, Yoshihiro Nakajima, Masanori Horie
Phagocytosis is a physiological process used by immune cells such as macrophages to actively ingest and destroy foreign pathogens and particles. It is the cellular process that leads to the failure of drug delivery carriers because the drug carriers are cleared by immune cells before reaching their target. Therefore, clarifying the mechanism of particle phagocytosis would have a significant implication for both fundamental understanding and biomedical engineering. As far as we know, the effect of particle shape on biological response has not been fully investigated...
June 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28623807/hiv-tat-excites-d1-receptor-like-expressing-neurons-from-rat-nucleus-accumbens
#16
G Cristina Brailoiu, Elena Deliu, Jeffrey L Barr, Linda M Console-Bram, Alexandra M Ciuciu, Mary E Abood, Ellen M Unterwald, Eugen Brailoiu
BACKGROUND: HIV-1 infection and drug abuse are frequently co-morbid and their association greatly increases the severity of HIV-1-induced neuropathology. While nucleus accumbens (NAcc) function is severely perturbed by drugs of abuse, little is known about how HIV-1 infection affects NAcc. METHODS: We used calcium and voltage imaging to investigate the effect of HIV-1 trans-activator of transcription (Tat) on rat NAcc. Based on previous neuronal studies, we hypothesized that Tat modulates intracellular Ca(2+) homeostasis of NAcc neurons...
June 8, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28623463/hypotonicity-induced-cell-swelling-activates-trpa1
#17
Fumitaka Fujita, Kunitoshi Uchida, Yasunori Takayama, Yoshiro Suzuki, Masayuki Takaishi, Makoto Tominaga
Hypotonic solutions can cause painful sensations in nasal and ocular mucosa through molecular mechanisms that are not entirely understood. We clarified the ability of human TRPA1 (hTRPA1) to respond to physical stimulus, and evaluated the response of hTRPA1 to cell swelling under hypotonic conditions. Using a Ca(2+)-imaging method, we found that modulation of AITC-induced hTRPA1 activity occurred under hypotonic conditions. Moreover, cell swelling in hypotonic conditions evoked single-channel activation of hTRPA1 in a cell-attached mode when the patch pipette was attached after cell swelling under hypotonic conditions, but not before swelling...
June 16, 2017: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/28623271/decoy-peptide-targeted-to-toll-il-1r-domain-inhibits-lps-and-tlr4-active-metabolite-morphine-3-glucuronide-sensitization-of-sensory-neurons
#18
Yohance M Allette, Youngsook Kim, Aaron L Randolph, Jared A Smith, Matthew S Ripsch, Fletcher A White
Accumulating evidence indicates that Toll-like receptor (TLR) signaling adapter protein interactions with Toll/Interleukin-1 Receptor (TIR) domains present in sensory neurons may modulate neuropathic pain states. Following ligand interaction with TLRs, TIR serves to both initiate intracellular signaling and facilitate recruitment of signaling adapter proteins to the intracytoplasmic domain. Although TLR TIR is central to a number of TLR signaling cascades, its role in sensory neurons is poorly understood. In this study we investigated the degree to which TLR TIR decoy peptide modified to include a TAT sequence (Trans-Activator of Transcription gene in HIV; TAT-4BB) affected LPS-induced intracellular calcium flux and excitation in sensory neurons, and behavioral changes due to TLR4 active metabolite, morphine-3-glucuronide (M3G) exposure in vivo...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622523/intracellular-ca-2-sensing-its-role-in-calcium-homeostasis-and-signaling
#19
REVIEW
Rafaela Bagur, György Hajnóczky
Ca(2+) is a ubiquitous intracellular messenger that controls diverse cellular functions but can become toxic and cause cell death. Selective control of specific targets depends on spatiotemporal patterning of the calcium signal and decoding it by multiple, tunable, and often strategically positioned Ca(2+)-sensing elements. Ca(2+) is detected by specialized motifs on proteins that have been biochemically characterized decades ago. However, the field of Ca(2+) sensing has been reenergized by recent progress in fluorescent technology, genetics, and cryo-EM...
June 15, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28622300/the-als-linked-e102q-mutation-in-sigma-receptor-1-leads-to-er-stress-mediated-defects-in-protein-homeostasis-and-dysregulation-of-rna-binding-proteins
#20
Alice Dreser, Jan Tilmann Vollrath, Antonio Sechi, Sonja Johann, Andreas Roos, Alfred Yamoah, Istvan Katona, Saeed Bohlega, Dominik Wiemuth, Yuemin Tian, Axel Schmidt, Jörg Vervoorts, Marc Dohmen, Cordian Beyer, Jasper Anink, Eleonora Aronica, Dirk Troost, Joachim Weis, Anand Goswami
Amyotrophic lateral sclerosis (ALS) is characterized by the selective degeneration of motor neurons (MNs) and their target muscles. Misfolded proteins which often form intracellular aggregates are a pathological hallmark of ALS. Disruption of the functional interplay between protein degradation (ubiquitin proteasome system and autophagy) and RNA-binding protein homeostasis has recently been suggested as an integrated model that merges several ALS-associated proteins into a common pathophysiological pathway...
June 16, 2017: Cell Death and Differentiation
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