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https://www.readbyqxmd.com/read/29164417/native-promoters-of-corynebacterium-glutamicum-and-its-application-in-l-lysine-production
#1
Xiuling Shang, Xin Chai, Xuemei Lu, Yuan Li, Yun Zhang, Guoqiang Wang, Chen Zhang, Shuwen Liu, Yu Zhang, Jiyin Ma, Tingyi Wen
OBJECTIVE: To identify useful native promoters of Corynebacterium glutamicum for fine-tuning of gene expression in metabolic engineering. RESULTS: Sixteen native promoters of C. glutamicum were characterized. These promoters covered a strength range of 31-fold with small increments and exhibited relatively stable activity during the whole growth phase using β-galactosidase as the reporter. The mRNA level and enzymatic activity of the lacZ reporter gene exhibited high correlation (R (2) = 0...
November 21, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29163459/crispr-cas9-system-as-a-valuable-genome-editing-tool-for-wine-yeasts-with-application-to-decrease-urea-production
#2
Ileana Vigentini, Marinella Gebbia, Alessandra Belotti, Roberto Foschino, Frederick P Roth
An extensive repertoire of molecular tools is available for genetic analysis in laboratory strains of S. cerevisiae. Although this has widely contributed to the interpretation of gene functionality within haploid laboratory isolates, the genetics of metabolism in commercially-relevant polyploid yeast strains is still poorly understood. Genetic engineering in industrial yeasts is undergoing major changes due to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) engineering approaches...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163398/phytohormones-and-beneficial-microbes-essential-components-for-plants-to-balance-stress-and-fitness
#3
REVIEW
Dilfuza Egamberdieva, Stephan J Wirth, Abdulaziz A Alqarawi, Elsayed F Abd Allah, Abeer Hashem
Plants are subjected to various abiotic stresses, such as drought, extreme temperature, salinity, and heavy metals. Abiotic stresses have negative impact on the physiology and morphology of plants through defects in the genetic regulation of cellular pathways. Plants employ several tolerance mechanisms and pathways to avert the effects of stresses that are triggered whenever alterations in metabolism are encountered. Phytohormones are among the most important growth regulators; they are known for having a prominent impact on plant metabolism, and additionally, they play a vital role in the stimulation of plant defense response mechanisms against stresses...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29162461/compressed-collagen-constructs-with-optimized-mechanical-properties-and-cell-interactions-for-tissue-engineering-applications
#4
Fatemeh Ajalloueian, Nikolaos Nikogeorgos, Ali Ajalloueian, Magdalena Fossum, Seunghwan Lee, Ioannis S Chronakis
In this study, we are introducing a simple, fast and reliable add-in to the technique of plastic compression (PC) to obtain collagen sheets with decreased fibrillar densities, representing improved cell-interactions and mechanical properties. Collagen hydrogels with different initial concentrations (1.64mg/mL-0.41mg/mL) were compressed around an electrospun sheet of PLGA. The scaffolds were then studied as non-seeded, or seeded with 3T3 fibroblast cells and cultured for 7 days. Confocal microscopy and TEM imaging of non-seeded scaffolds showed that by decreasing the share of collagen in the hydrogel formula, collagen sheets with similar thickness but lower fibrous densities were achieved...
November 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29160039/fine-tuning-the-p-pastoris-imt1026-genome-scale-metabolic-model-for-improved-prediction-of-growth-on-methanol-or-glycerol-as-sole-carbon-sources
#5
Màrius Tomàs-Gamisans, Pau Ferrer, Joan Albiol
The methylotrophic yeast Pichia pastoris (Komagataella spp.) is widely used as cell factory for recombinant protein production. In the past recent years, important breakthroughs in the systems-level quantitative analysis of its physiology have been achieved. This wealth of information has allowed the development of genome-scale metabolic models, which make new approaches possible for host cell and bioprocess engineering. Nevertheless, the predictive accuracy of the previous consensus model required to be upgraded and validated with new experimental data sets for P...
November 21, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29159585/kfoa-the-udp-glucose-4-epimerase-of-escherichia-coli-strain-o5-k4-h4-shows-preference-for-acetylated-substrates
#6
Hao-Miao Zhu, Bin Sun, Yi-Jun Li, Dan-Hua Meng, Shuang Zheng, Ting-Ting Wang, Feng-Shan Wang, Ju-Zheng Sheng
Capsule of Escherichia coli O5:K4:H4 is formed of a chondroitin-repeat disaccharide unit of glucuronic acid (GlcA)-N-acetylgalactosamine (GalNAc). This polysaccharide, commonly referred to as K4CP, is a potentially important source of precursors for chemoenzymatic or bioengineering synthesis of chondroitin sulfate. KfoA, encoded by a gene from region 2 of the K4 capsular gene cluster, shows high homology to the UDP-glucose-4-epimerase (GalE) from E. coli. KfoA is reputed to be responsible for uridine 5'-diphosphate-N-acetylgalactosamine (UDP-GalNAc) supply for K4CP biosynthesis in vivo, but it has not been biochemically characterized...
November 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29159508/comparative-genomics-and-transcriptome-analysis-of-lactobacillus-rhamnosus-atcc-11443-and-the-mutant-strain-sct-10-10-60-with-enhanced-l-lactic-acid-production-capacity
#7
Liang Sun, Zhilong Lu, Jianxiu Li, Feifei Sun, Ribo Huang
Mechanisms for high L-lactic acid production remain unclear in many bacteria. Lactobacillus rhamnosus SCT-10-10-60 was previously obtained from L. rhamnosus ATCC 11443 via mutagenesis and showed improved L-lactic acid production. In this study, the genomes of strains SCT-10-10-60 and ATCC 11443 were sequenced. Both genomes are a circular chromosome, 2.99 Mb in length with a GC content of approximately 46.8%. Eight split genes were identified in strain SCT-10-10-60, including two LytR family transcriptional regulators, two Rex redox-sensing transcriptional repressors, and four ABC transporters...
November 20, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29158974/whole-genome-sequencing-of-rhodotorula-mucilaginosa-isolated-from-the-chewing-stick-distemonanthus-benthamianus-insights-into-rhodotorula-phylogeny-mitogenome-dynamics-and-carotenoid-biosynthesis
#8
Han Ming Gan, Bolaji N Thomas, Nicole T Cavanaugh, Grace H Morales, Ashley N Mayers, Michael A Savka, André O Hudson
In industry, the yeast Rhodotorula mucilaginosa is commonly used for the production of carotenoids. The production of carotenoids is important because they are used as natural colorants in food and some carotenoids are precursors of retinol (vitamin A). However, the identification and molecular characterization of the carotenoid pathway/s in species belonging to the genus Rhodotorula is scarce due to the lack of genomic information thus potentially impeding effective metabolic engineering of these yeast strains for improved carotenoid production...
2017: PeerJ
https://www.readbyqxmd.com/read/29158189/development-of-an-oxygen-independent-flavin-mononucleotide-based-fluorescent-reporter-system-in-clostridium-beijerinckii-and-its-applications
#9
Seung-Oh Seo, Ting Lu, Yong-Su Jin, Hans P Blaschek
Clostridium beijerinckii is a predominant solventogenic clostridia with great attraction for renewable liquid biofuel and biochemical production. Metabolic engineering and synthetic biology can be employed to engineer the strain toward desirable phenotypes. However, current limited information such as promoter strength and gene regulation may hinder the efficient engineering of strain. To investigate genetic information and complex cellular bioprocesses of C. beijerinckii, in vivo fluorescence reporter system can be employed...
November 17, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29157236/monitoring-of-nutrient-limitation-in-growing-e-coli-a-mathematical-model-of-a-ppgpp-based-biosensor
#10
Alexandra Pokhilko
BACKGROUND: E. coli can be used as bacterial cell factories for production of biofuels and other useful compounds. The efficient production of the desired products requires careful monitoring of growth conditions and the optimization of metabolic fluxes. To avoid nutrient depletion and maximize product yields we suggest using a natural mechanism for sensing nutrient limitation, related to biosynthesis of an intracellular messenger - guanosine tetraphosphate (ppGpp). RESULTS: We propose a design for a biosensor, which monitors changes in the intracellular concentration of ppGpp by coupling it to a fluorescent output...
November 21, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/29155060/controlling-cell-free-metabolism-through-physiochemical-perturbations
#11
Ashty S Karim, Jacob T Heggestad, Samantha A Crowe, Michael C Jewett
Building biosynthetic pathways and engineering metabolic reactions in cells can be time-consuming due to complexities in cellular metabolism. These complexities often convolute the combinatorial testing of biosynthetic pathway designs needed to define an optimal biosynthetic system. To simplify the optimization of biosynthetic systems, we recently reported a new cell-free framework for pathway construction and testing. In this framework, multiple crude-cell extracts are selectively enriched with individual pathway enzymes, which are then mixed to construct full biosynthetic pathways on the time scale of a day...
November 15, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/29154907/an-integrated-mathematical-epithelial-cell-model-for-airway-surface-liquid-regulation-by-mechanical-forces
#12
Dan Wu, Richard C Boucher, Brian Button, Timothy Elston, Ching-Long Lin
A robust method based on reverse engineering was utilized to construct the ion-channel conductance functions for airway epithelial sodium channels (ENaC), the cystic fibrosis transmembrane conductance regulator (CFTR), and calcium-activated chloride channels (CaCC). The ion-channel conductance models for both normal (NL) and cystic fibrosis (CF) airway epithelia were developed and then coupled to an adenosine triphosphate (ATP) metabolism model and a fluid transport model (collectively called the integrated cell model) to investigate airway surface liquid (ASL) volume regulation and hence mucus concentration, by mechanical forces in NL and CF human airways...
November 15, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29153651/enhanced-l-ornithine-production-by-systematic-manipulation-of-l-ornithine-metabolism-in-engineered-corynebacterium-glutamicum-s9114
#13
Bin Zhang, Li-Quan Ren, Miao Yu, Ying Zhou, Bang-Ce Ye
l-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, l-ornithine is produced by microbial fermentation; however, this process needs to be further improved in terms of l-ornithine productivity and cost reduction. In this study, overexpression of LysE was observed to increase l-ornithine production in engineered Corynebacterium glutamicum S9114. To overcome the drawbacks of using a plasmid to express LysE, Ptac, a strong promoter, was inserted in the upstream region of lysE on the chromosome...
November 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29151887/an-accurate-description-of-aspergillus-niger-organic-acid-batch-fermentation-through-dynamic-metabolic-modelling
#14
Daniel J Upton, Simon J McQueen-Mason, A Jamie Wood
Background: Aspergillus niger fermentation has provided the chief source of industrial citric acid for over 50 years. Traditional strain development of this organism was achieved through random mutagenesis, but advances in genomics have enabled the development of genome-scale metabolic modelling that can be used to make predictive improvements in fermentation performance. The parent citric acid-producing strain of A. niger, ATCC 1015, has been described previously by a genome-scale metabolic model that encapsulates its response to ambient pH...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29151886/optogenetic-regulation-of-artificial-microrna-improves-h2-production-in-green-alga-chlamydomonas-reinhardtii
#15
Yuting Wang, Xinqin Jiang, Changxing Hu, Ting Sun, Zhiyong Zeng, Xiaoqi Cai, Hui Li, Zhangli Hu
Background: Chlamydomonas reinhardtii is an ideal model organism not only for the study of basic metabolic processes in both plants and animals but also the production of biofuels including hydrogen. Transgenic analysis of C. reinhardtii is now well established and very convenient, but inducible exogenous gene expression systems remain under-studied. The most commonly used heat shock-inducible system has serious effects on algal cell growth and is difficult and costly to control in large-scale culture...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29151638/polyhydroxyalkanoate-production-and-degradation-patterns-in-bacillus-species
#16
Subhasree Ray, Vipin Chandra Kalia
Bacteria under stress conditions of excess of carbon (C) and limitations of nutrients divert its metabolism towards C storage as energy reservoir-polyhydroxyalkanoate (PHA). Different Bacillus species-B. cereus and B. thuringiensis, were monitored to produce PHA from different C sources-glucose, crude glycerol and their combination at 37 °C for period up to 192 h. PHA production and its composition was found to vary with feed and bacterial strains. PHA production on crude glycerol continued to increase up to 120 h, reaching a maximum of 2725 mg/L with an effective yield of 71% of the dry cell mass...
December 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29151637/strategies-to-improve-saccharomyces-cerevisiae-technological-advancements-and-evolutionary-engineering
#17
REVIEW
Arun Kumar Dangi, Kashyap Kumar Dubey, Pratyoosh Shukla
Bakery industries are thriving to augment the diverse properties of Saccharomyces cerevisiae to increase its flavor, texture and nutritional parameters to attract the more consumers. The improved technologies adopted for quality improvement of baker's yeast are attracting the attention of industry and it is playing a pivotal role in redesigning the quality parameters. Modern yeast strain improvement tactics revolve around the use of several advanced technologies such as evolutionary engineering, systems biology, metabolic engineering, genome editing...
December 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29150723/a-grapevine-inducible-gene-vv-%C3%AE-gal-sip-confers-salt-and-desiccation-tolerance-in-escherichia-coli-and-tobacco-at-germinative-stage
#18
Samia Daldoul, Anis Ben Amar, Mahmoud Gargouri, Hajer Limam, Ahmed Mliki, Thierry Wetzel
Grapevine is an important fruit crop cultivated worldwide. Previously, we have reported the characterization of a salt stress-inducible gene Vv-α-gal/SIP isolated from the tolerant grapevine cultivar Razegui. In this study, we performed functional studies in both Escherichia coli and tobacco systems to gain more insights in the role of the Vv-α-gal/SIP gene. Our data revealed that the recombinant E. coli cells harboring the pET24b(+) expression vector with the Vv-α-gal/SIP showed higher tolerance to desiccation and salinity compared to E...
November 17, 2017: Biochemical Genetics
https://www.readbyqxmd.com/read/29150516/drna-seq-implicates-sulfide-as-master-regulator-of-s-0-metabolism-in-chlorobaculum-tepidum-and-other-green-sulfur-bacteria
#19
Jacob M Hilzinger, Vidhyavathi Raman, Kevin E Shuman, Brian J Eddie, Thomas E Hanson
The green sulfur bacteria (Chlorobiaceae) are anaerobes that use electrons from reduced sulfur compounds (sulfide, S(0), and thiosulfate) as electron donors for photoautotrophic growth. Chlorobaculum tepidum, the model system for the Chlorobiaceae, both produces and consumes extracellular S(0) globules depending on the availability of sulfide in the environment. These physiological changes imply significant changes in gene regulation, which has been observed when sulfide is added to Cba. tepidum growing on thiosulfate...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150512/a-genetic-system-for-the-thermophilic-acetogenic-bacterium-thermoanaerobacter-kivui
#20
Mirko Basen, Irina Geiger, Laura Henke, Volker Müller
Thermoanaerobacter kivui is one of the very few thermophilic acetogenic microorganisms. It grows optimally at 66°C on sugars but also lithotrophically with H2 + CO2 or with CO, producing acetate as major product. While a genome-derived model of acetogenesis has been developed, only few physiological or biochemical experiments towards the function of important enzymes in carbon and energy metabolism have been carried out. To address this issue, we developed a method for targeted, markerless gene deletions and for integration of genes into the genome of T...
November 17, 2017: Applied and Environmental Microbiology
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