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https://www.readbyqxmd.com/read/29350404/gastrointestinal-protists-and-helminths-of-habituated-agile-mangabeys-cercocebus-agilis-at-bai-hokou-central-african-republic
#1
Barbora Pafčo, Zuzana Tehlárová, Kateřina Jirků Pomajbíková, Angelique Todd, Hideo Hasegawa, Klára J Petrželková, David Modrý
Infectious diseases including those caused by parasites can be a major threat to the conservation of endangered species. There is thus a great need for studies describing parasite infections of these species in the wild. Here we present data on parasite diversity in an agile mangabey (Cercocebus agilis) group in Bai Hokou, Dzanga-Sangha Protected Areas (DSPA), Central African Republic. We coproscopically analyzed 140 mangabey fecal samples by concentration techniques (flotation and sedimentation). Agile mangabeys hosted a broad diversity of protistan parasites/commensals, namely amoebas (Entamoeba spp...
January 19, 2018: American Journal of Primatology
https://www.readbyqxmd.com/read/29348580/the-epibiotic-life-of-the-cosmopolitan-diatom-fragilariopsis-doliolus-on-heterotrophic-ciliates-in-the-open-ocean
#2
Flora J Vincent, Sébastien Colin, Sarah Romac, Eleonora Scalco, Lucie Bittner, Yonara Garcia, Rubens M Lopes, John R Dolan, Adriana Zingone, Colomban de Vargas, Chris Bowler
Diatoms are a diverse and ecologically important group of phytoplankton. Although most species are considered free living, several are known to interact with other organisms within the plankton. Detailed imaging and molecular characterization of any such partnership is, however, limited, and an appraisal of the large-scale distribution and ecology of such consortia was never attempted. Here, observation of Tara Oceans samples from the Benguela Current led to the detection of an epibiotic association between a pennate diatom and a tintinnid ciliate...
January 18, 2018: ISME Journal
https://www.readbyqxmd.com/read/29339818/development-and-optimization-of-a-differentiated-airway-epithelial-cell-model-of-the-bovine-respiratory-tract
#3
Daniel Cozens, Edward Grahame, Erin Sutherland, Geraldine Taylor, Catherine C Berry, Robert L Davies
Cattle are subject to economically-important respiratory tract infections by various bacterial and viral pathogens and there is an urgent need for the development of more realistic in vitro models of the bovine respiratory tract to improve our knowledge of disease pathogenesis. In the present study, we have optimized the culture conditions in serum-free medium that allow bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface to differentiate into a three-dimensional epithelium that is highly representative of the bovine airway...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29335229/ciliated-cyst-of-the-gallbladder-a-new-case-and-literature-review
#4
Triki Wissem, Baraket Oussema, Itami Ahmed, Baccar Abdelmajid, Marzouk Ines, Bouchoucha Sami
INTRODUCTION: The ciliated cyst is a rare anomaly that develops from the anterior primitive intestine. Its localization is essentially supra diaphragmatic. It's localization in the gallbladder is very uncommon. CASE REPORT: We report the first case in Tunisia of a ciliated cyst of the gallbladder in a 34 years old woman who was operated for a gallbladder stone and in whom the discovery of a cystic mass attached to the neck of the gallbladder was preoperatively. The pathologic study did conclude to a ciliated cyst...
December 21, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29331864/taxonomic-studies-on-aegyria-apoliva-sp-nov-and-trithigmostoma-cucullulus-m%C3%A3-ller-1786-jankowski-1967-ciliophora-cyrtophoria-with-phylogenetic-analyses
#5
Xumiao Chen, Lifang Li, Saleh A Al-Farraj, Honggang Ma, Hongbo Pan
The morphology, including the ciliary pattern, of two cyrtophorid ciliates, namely Aegyria apoliva sp. nov. and Trithigmostoma cucullulus (Müller, 1786) Jankowski, 1967, were investigated. They were isolated from coastal water off Qingdao and from an estuarine habitat in south China, respectively. Aegyria apoliva sp. nov. is characterized as following: body size 100-120 × 50-70 μm in vivo with dark pigment spot on anterior left part of cell; 48-69 somatic kineties; one preoral and four or five circumoral kineties; 9-13 transpodial segments; 26-40 nematodesmal rods; 6-10 contractile vacuoles...
December 28, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/29330013/a-functional-splicing-variant-associated-with-decreased-asthma-risk-abolishes-the-ability-of-gasdermin-b-gsmdb-to-induce-epithelial-cell-pyroptosis
#6
Ronald A Panganiban, Maoyun Sun, Amber Dahlin, Hae-Ryung Park, Mengyuan Kan, Blanca E Himes, Jennifer A Mitchel, Carlos Iribarren, Eric Jorgenson, Scott H Randell, Elliot Israel, Kelan Tantisira, Stephanie Shore, Jin-Ah Park, Scott T Weiss, Ann Chen Wu, Quan Lu
BACKGROUND: Genetic variants in the chromosomal region 17q21 are consistently associated with asthma. However, mechanistic studies have not yet linked any of the associated variants to a function that could influence asthma, and as a result, the identity of the asthma gene(s) remains elusive. OBJECTIVES: We sought to identify and characterize functional variants in the 17q21 locus. METHODS: We used the Exome Aggregation Consortium (ExAC) browser to identify coding (amino acid-changing) variants in the 17q21 locus...
January 9, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29324899/the-effects-of-high-dose-of-two-manganese-supplements-organic-and-inorganic-on-the-rumen-microbial-ecosystem
#7
Svetlana Kišidayová, Peter Pristaš, Michaela Zimovčáková, Monika Blanár Wencelová, Lucia Homol'ová, Katarína Mihaliková, Klaudia Čobanová, Ľubomíra Grešáková, Zora Váradyová
Little is known about the effects of the high dose and types of manganese supplements on rumen environment at manganese intake level close above the limit of 150 mg/kg of dry feed matter. The effects of high dose of two manganese supplements (organic and inorganic) on rumen microbial ecosystem after four months of treatment of 18 lambs divided into three treatment groups were studied. We examined the enzyme activities (α-amylase, xylanase, and carboxymethyl cellulase), total and differential microscopic counts of rumen ciliates, total microscopic counts of bacteria, and fingerprinting pattern of the eubacterial and ciliates population analyzed by PCR-DGGE...
2018: PloS One
https://www.readbyqxmd.com/read/29321663/lpa-signaling-is-regulated-through-the-primary-cilium-a-novel-target-in-glioblastoma
#8
Yuriy V Loskutov, Caryn L Griffin, Kristina M Marinak, Andrey Bobko, Naira V Margaryan, Werner J Geldenhuys, Jann N Sarkaria, Elena N Pugacheva
The primary cilium is a ubiquitous organelle presented on most human cells. It is a crucial signaling hub for multiple pathways including growth factor and G-protein coupled receptors. Loss of primary cilia, observed in various cancers, has been shown to affect cell proliferation. Primary cilia formation is drastically decreased in glioblastoma (GBM), however, the role of cilia in normal astrocyte or glioblastoma proliferation has not been explored. Here, we report that loss of primary cilia in human astrocytes stimulates growth rate in a lysophosphatidic acid (LPA)-dependent manner...
January 11, 2018: Oncogene
https://www.readbyqxmd.com/read/29317511/exploration-of-the-germline-genome-of-the-ciliate-chilodonella-uncinata-through-single-cell-omics-transcriptomics-and-genomics
#9
Xyrus X Maurer-Alcalá, Rob Knight, Laura A Katz
Separate germline and somatic genomes are found in numerous lineages across the eukaryotic tree of life, often separated into distinct tissues (e.g., in plants, animals, and fungi) or distinct nuclei sharing a common cytoplasm (e.g., in ciliates and some foraminifera). In ciliates, germline-limited (i.e., micronuclear-specific) DNA is eliminated during the development of a new somatic (i.e., macronuclear) genome in a process that is tightly linked to large-scale genome rearrangements, such as deletions and reordering of protein-coding sequences...
January 9, 2018: MBio
https://www.readbyqxmd.com/read/29316045/redescription-of-tintinnopsis-everta-kofoid-campbell-1929-alveolata-ciliophora-tintinnina-based-on-taxonomic-and-genetic-analyses-discovery-of-a-new-complex-ciliary-pattern
#10
Michael S Gruber, Michaela Strüder-Kypke, Sabine Agatha
The about 1,000 species of tintinnid ciliates are identified and classified almost exclusively based on their lorica features, although the shortcomings of this structure are well-known, e.g., causing uncertain species limitations and non-monophyletic taxa. Hence, the present redescription of Tintinnopsis everta Kofoid and Campbell, 1929 considers not only the lorica characteristics, but focuses on cell and genetic features. The species is redescribed from the North Atlantic and adjacent sea areas, namely the east coast of the USA, using live observation, protargol-stained material, scanning electron microscopy, and genetic analyses...
January 5, 2018: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/29315959/morphological-and-molecular-redefinition-of-euplotes-platystoma-dragesco-dragesco-kern%C3%A3-is-1986-and-aspidisca-lynceus-m%C3%A3-ller-1773-ehrenberg-1830-with-reconsideration-of-a-well-known-euplotes-ciliate-euplotes-harpa-stein-1859-ciliophora-euplotida
#11
Chunyu Lian, Xiaotian Luo, Xinpeng Fan, Jie Huang, Yuhe Yu, William Bourland, Weibo Song
We documented the morphology, infraciliature, silverline system and molecular data of two euplotid species isolated from China, including two populations of the poorly known Euplotes platystoma Dragesco & Dragesco-Kernéis, 1986 and the previously well described Aspidisca lynceus (Müller, 1773) Ehrenberg, 1830. Based on the information available, an improved diagnosis of Euplotes platystoma is given, including: a narrow adoral zone with 44-68 membranelles, ten frontoventral, five transverse, two left marginal and two caudal cirri, 11-13 dorsal kineties with 17-25 dikinetids in the mid-dorsal row, and dorsal silverline system of the double-eurystomus type...
January 9, 2018: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/29315711/histological-histochemical-and-ultrastructural-investigation-of-the-male-copulatory-apparatus-of-haminoea-navicula-gastropoda-cephalaspidea
#12
Alexandre Lobo-da-Cunha, Ângela Alves, Elsa Oliveira, Manuel António E Malaquias
Due to its biological and systematic importance, the morphology and function of the male copulatory apparatus of Haminoea navicula, a Cephalaspidea gastropod mollusk, was investigated by light and electron microscopy. These systems are poorly understood in haminoids, but are often used in the taxonomy of the genus. In H. navicula, the male copulatory apparatus comprises the penis within a penial sheath, a seminal duct and the prostate with two lobes. The penis is a muscular structure with a tip covered by spikes formed by muscular cells...
January 8, 2018: Journal of Morphology
https://www.readbyqxmd.com/read/29314766/gpsit-an-automated-method-for-evolutionary-analysis-of-nonculturable-ciliated-microeukaryotes
#13
Xiao Chen, Yurui Wang, Yalan Sheng, Alan Warren, Shan Gao
Microeukaryotes are among the most important components of the microbial food web in almost all aquatic and terrestrial ecosystems worldwide. In order to gain a better understanding their roles and functions in ecosystems, sequencing coupled with phylogenomic analyses of entire genomes or transcriptomes are increasingly used to reconstruct the evolutionary history and classification of these microeukaryotes and thus provide a more robust framework for determining their systematics and diversity. More importantly, phylogenomic research usually requires high levels of hands-on bioinformatics experience...
January 4, 2018: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29312762/ciliated-muconodular-papillary-tumor-of-the-lung-harboring-braf-v600e-mutation-and-p16ink4a-overexpression-without-proliferative-activity-may-represent-an-example-of-oncogene-induced-senescence
#14
Lucia Kim, Young Sam Kim, Jin Soo Lee, Suk Jin Choi, In Suh Park, Jee Young Han, Joon Mee Kim, Young Chae Chu
Ciliated muconodular papillary tumor (CMPT) is a rare peripheral lung tumor that shows puzzling histologic features encompassing metaplastic and neoplastic nature. This type of tumor is occasionally misdiagnosed as lung adenocarcinoma clinically and pathologically, and its pathogenic mechanism has not been well characterized. We experienced a case of CMPT in a 73-year-old male and performed targeted deep sequencing to characterize its molecular features. The tumor was an ill-defined, subpleural, and non-endobronchial nodule showing glandular and papillary proliferation of mucous cells, ciliated columnar cells, and basal cells without any cytologic atypia...
December 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29311494/immunohistochemical-study-on-the-distribution-of-%C3%AE-defensin-1-and-%C3%AE-defensin-2-throughout-the-respiratory-tract-of-healthy-rats
#15
Natsumi Masuda, Youhei Mantani, Hideto Yuasa, Chiaki Yoshitomi, Masaya Arai, Miho Nishida, Wang-Mei Qi, Junichi Kawano, Toshifumi Yokoyama, Nobuhiko Hoshi, Hiroshi Kitagawa
The distributions of β-defensin 1 and 2 in secretory host defense system throughout respiratory tract of healthy rats were immunohistochemically investigated. In the nasal epithelium, a large number of non-ciliated and non-microvillous cells (NCs) were immunopositive for both β-defensin 1 and 2, whereas a small number of goblet cells (GCs) were immunopositive only for β-defensin 1. Beta-defensin 2-immunopositive GCs were few. In the nasal glands, a small number of acinar cells and a large number of ductal epithelial cells were immunopositive for both β-defensins...
January 1, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29305615/primary-cilia-proteins-ciliary-and-extraciliary-sites-and-functions
#16
REVIEW
Kiet Hua, Russell J Ferland
Primary cilia are immotile organelles known for their roles in development and cell signaling. Defects in primary cilia result in a range of disorders named ciliopathies. Because this organelle can be found singularly on almost all cell types, its importance extends to most organ systems. As such, elucidating the importance of the primary cilium has attracted researchers from all biological disciplines. As the primary cilia field expands, caution is warranted in attributing biological defects solely to the function of this organelle, since many of these "ciliary" proteins are found at other sites in cells and likely have non-ciliary functions...
January 5, 2018: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29303074/olfactory-loss-and-dysfunction-in-ciliopathies-molecular-mechanisms-and-potential-therapies
#17
Cedric R Uytingco, Warren W Green, Jeffrey R Martens
Ciliopathies are a class of inherited pleiotropic genetic disorders in which alterations in cilia assembly, maintenance, and/or function exhibit penetrance in the multiple organ systems. Olfactory dysfunction is one such clinical manifestation that has been shown in both patients and model organisms. Existing therapies for ciliopathies are limited to the treatment or management of symptoms. The last decade has seen an increase in potential curative therapeutic options including small molecules and biologics...
January 4, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29301909/an-expanded-role-for-the-rfx-transcription-factor-daf-19-with-dual-functions-in-ciliated-and-non-ciliated-neurons
#18
Elizabeth A De Stasio, Katherine P Mueller, Rosemary J Bauer, Alexander J Hurlburt, Sophie A Bice, Sophie L Scholtz, Prasad Phirke, Debora Sugiaman-Trapman, Loraina A Stinson, Haili B Olson, Savannah L Vogel, Zabdiel Ek-Vazquez, Yagmur Esemen, Jessica Korzynski, Kelsey Wolfe, Bonnie N Arbuckle, He Zhang, Gaelen Lombard-Knapp, Brian P Piasecki, Peter Swoboda
Regulatory Factor X transcription factors (RFX TFs) are best known for activating genes required for ciliogenesis in both vertebrates and invertebrates. In humans, eight RFX TFs have a variety of tissue-specific functions, while in the worm Caenorhabditis elegans, the sole RFX gene, daf-19, encodes a set of nested isoforms. Null alleles of daf-19 confer pleiotropic effects including altered development with a dauer constitutive phenotype, complete absence of cilia and ciliary proteins, and defects in synaptic protein maintenance...
January 3, 2018: Genetics
https://www.readbyqxmd.com/read/29298171/expression-of-%C3%AE-v-tubulin-in-secretory-cells-of-the-fallopian-tube-epithelium-marks-cellular-atypia
#19
Deepti Mathew, Yanhua Wang, Anne Van Arsdale, Susan Band Horwitz, Hayley McDaid
OBJECTIVES: Class V Beta tubulin isotype (βV-tubulin) was recently found to have tissue-specific expression patterns in epithelial tissues with secretory function and aberrant expression in tumors. The aims of this pilot study were (a) to examine expression of βV-tubulin in the fallopian tube epithelium (FTE) of patients who underwent salpingectomy, (b) to characterize FTE atypia in high-risk patients with BRCA mutations, and (c) to determine expression of βV-tubulin in serous ovarian neoplasms...
January 3, 2018: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/29295610/effect-of-rhodophyta-extracts-on-in-vitro-ruminal-fermentation-characteristics-methanogenesis-and-microbial-populations
#20
Shin Ja Lee, Nyeon Hak Shin, Jin Suk Jeong, Eun Tae Kim, Su Kyoung Lee, Sung Sill Lee
OBJECTIVE: Due to the threat of global warming, the livestock industry is increasingly interested in exploring how feed additives may reduce anthropogenic greenhouse gas emissions, especially from ruminants. This study investigated the effect of Rhodophyta supplemented bovine diets on in vitro rumen fermentation and rumen microbial diversity. METHODS: Cannulated Holstein cows were used as rumen fluid donors. Rumen fluid:buffer (1:2; 15 mL) solution was incubated for up to 72 h in six treatments: a control (timothy hay only), along with substrates containing 5% extracts from five Rhodophyta species (Grateloupia lanceolata [Okamura] Kawaguchi, Hypnea japonica Tanaka, Pterocladia capillacea [Gmelin] Bornet, Chondria crassicaulis Harvey, or Gelidium amansii [Lam...
January 2018: Asian-Australasian Journal of Animal Sciences
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