keyword
MENU ▼
Read by QxMD icon Read
search

monooxygenase

keyword
https://www.readbyqxmd.com/read/27913871/n2o-emission-characteristics-and-its-affecting-factors-in-rain-fed-potato-fields-in-wuchuan-county-china
#1
Liwei Wang, Cheng Wang, Zhihua Pan, Hui Xu, Lin Gao, Peiyi Zhao, Zhiqiang Dong, Jingting Zhang, Guohui Cui, Sen Wang, Guolin Han, Hui Zhao
Representing an important greenhouse gas, nitrous oxide (N2O) emission from cultivated land is a hot topic in current climate change research. This study examined the influences of nitrogen fertilisation, temperature and soil moisture on the ammonia monooxygenase subunit A (amoA) gene copy numbers and N2O emission characteristics. The experimental observation of N2O fluxes was based on the static chamber-gas chromatographic method. The ammonia-oxidising bacteria (AOB) and ammonia-oxidising archaea (AOA) gene copy numbers in different periods were measured by real-time polymerase chain reaction (PCR)...
December 3, 2016: International Journal of Biometeorology
https://www.readbyqxmd.com/read/27910771/induction-of-flavin-containing-monooxygenase-in-mice-by-oral-administration-of-phellinus-baumii-agaricomycetes-extract
#2
Batbayar Sainkhuu, Beom Su Park, Ha Won Kim
Phellinus baumii is a yellow mushroom long used in alternative medicine in Korea and other central Asian countries. To identify genes affected by a single or 7-day oral administration of a water extract of Ph. Baumii, mouse liver tissue was analyzed using microarrays. The results showed that 8 and 23 genes were upregulated and 3 and 11 genes downregulated more than 3-fold by single and multiple oral administrations of 100 mg/kg PBE, respectively. Among the upregulated genes, the expression of 3 flavin-containing monooxygenase (Fmo) family genes, Fmo2-4, was upregulated in a concentration-dependent manner...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27909346/copper-i-dioxygen-adducts-and-copper-enzyme-mechanisms
#3
Jeffrey J Liu, Daniel E Diaz, David A Quist, Kenneth D Karlin
Primary copper(I)-dioxygen (O2) adducts, cupric-superoxide complexes, have been proposed intermediates in copper-containing dioxygen-activating monooxygenase and oxidase enzymes. Here, mechanisms of C-H activation by reactive copper-(di)oxygen intermediates are discussed, with an emphasis on cupric-superoxide species. Over the past 25 years, many synthetically derived cupric-superoxide model complexes have been reported. Due to the thermal instability of these intermediates, early studies focused on increasing their stability and obtaining physical characterization...
October 2016: Israel Journal of Chemistry
https://www.readbyqxmd.com/read/27905614/methanobactins-from-genome-to-function
#4
Laura M K Dassama, Grace E Kenney, Amy C Rosenzweig
Methanobactins (Mbns) are ribosomally produced, post-translationally modified peptide (RiPP) natural products that bind copper with high affinity using nitrogen-containing heterocycles and thioamide groups. In some methanotrophic bacteria, Mbns are secreted under conditions of copper starvation and then re-internalized as a copper source for the enzyme particulate methane monooxygenase (pMMO). Genome mining studies have led to the identification and classification of operons encoding the Mbn precursor peptide (MbnA) as well as a number of putative transport, regulatory, and biosynthetic proteins...
December 1, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27904981/candida-guilliermondii-as-a-potential-biocatalyst-for-the-production-of-long-chain-%C3%AE-%C3%AF-dicarboxylic-acids
#5
Nicole Werner, Miriam Dreyer, Wenke Wagner, Nicolas Papon, Steffen Rupp, Susanne Zibek
OBJECTIVES: To explore Candida guilliermondii for the production of long-chain dicarboxylic acids (DCA), we performed metabolic pathway engineering aiming to prevent DCA consumption during β-oxidation, but also to increase its production via the ω-oxidation pathway. RESULTS: We identified the major β- and ω-oxidation pathway genes in C. guilliermondii and performed first steps in the strain improvement. A double pox disruption mutant was created that slowed growth with oleic acid but showed accelerated DCA degradation...
November 30, 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27900443/p450bm3-fused-to-phosphite-dehydrogenase-allows-phosphite-driven-selective-oxidations
#6
Nina Beyer, Justyna K Kulig, Anette Bartsch, Martin A Hayes, Dick B Janssen, Marco W Fraaije
To facilitate the wider application of the NADPH-dependent P450BM3, we fused the monooxygenase with a phosphite dehydrogenase (PTDH). The resulting monooxygenase-dehydrogenase fusion enzyme acts as a self-sufficient bifunctional catalyst, accepting phosphite as a cheap electron donor for the regeneration of NADPH.The well-expressed fusion enzyme was purified and analyzed in comparison to the parent enzymes. Using lauric acid as substrate for P450BM3, it was found that the fusion enzyme had similar substrate affinity and hydroxylation selectivity while it displayed a significantly higher activity than the non-fused monooxygenase...
November 29, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27898334/relative-contribution-of-ammonia-oxidizing-bacteria-and-other-members-of-nitrifying-activated-sludge-communities-to-micropollutant-biotransformation
#7
Yujie Men, Stefan Achermann, Damian E Helbling, David R Johnson, Kathrin Fenner
Improved micropollutant (MP) biotransformation during biological wastewater treatment has been associated with high ammonia oxidation activities, suggesting co-metabolic biotransformation by ammonia oxidizing bacteria as an underlying mechanism. The goal of this study was to clarify the contribution of ammonia oxidizing bacteria to increased MP degradation in nitrifying activated sludge (NAS) communities using a series of inhibition experiments. To this end, we treated a NAS community with two different ammonia oxidation inhibitors, namely octyne (OCT), a mechanistic inhibitor that covalently binds to ammonia monooxygenases, and allylthiourea (ATU), a copper chelator that depletes copper ions from the active center of ammonia monooxygenases...
November 22, 2016: Water Research
https://www.readbyqxmd.com/read/27894843/mir-509-5p-suppresses-the-proliferation-migration-and-invasion-of-non-small-cell-lung-cancer-by-targeting-ywhag
#8
Peng Wang, Yu Deng, XiangNing Fu
Dysregulation of microRNAs (miRNAs) expression is prevalent in human malignancy development and progression. As previously reported, miR-509-5p has been identified as a tumor suppressor in various cancers, but its role and functional mechanism in NSCLC remains unknown. In the present study, we found that miR-509-5p was significantly downregulated in non-small lung cancer (NSCLC) tissues and cell lines using Quantitative real-time PCR (qRT-PCR). In addition, overexpression of miR-509-5p markedly inhibited the proliferation, migration, invasion and phosphorylation of Akt of NSCLC cells...
November 25, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27889626/absence-of-kynurenine-3-monooxygenase-reduces-mortality-of-acute-viral-myocarditis-in-mice
#9
Hisako Kubo, Masato Hoshi, Akihiro Mouri, Chieko Tashita, Yasuko Yamamoto, Toshitaka Nabeshima, Kuniaki Saito
Infection of the encephalomyocarditis virus (EMCV) in mice is an established model for viral myocarditis. Previously, we have demonstrated that indoleamine 2,3-dioxygenase (IDO), an L-tryptophan - kynurenine pathway (KP) enzyme, affects acute viral myocarditis. However, the roles of KP metabolites in EMCV infection remain unclear. Kynurenine 3-monooxygenase (KMO) is one of the key regulatory enzymes, which metabolizes kynurenine to 3-hydroxykynurenine in the KP. Therefore, we examined the role of KMO in acute viral infection by comparing between KMO(-/-) mice and KMO(+/+) mice...
November 23, 2016: Immunology Letters
https://www.readbyqxmd.com/read/27883174/maximization-of-cell-viability-rather-than-biocatalyst-activity-improves-whole-cell-%C3%AF-oxyfunctionalization-performance
#10
Marvin Kadisch, Mattijs K Julsing, Manfred Schrewe, Nico Jehmlich, Benjamin Scheer, Martin von Bergen, Andreas Schmid, Bruno Bühler
It is a common misconception in whole-cell biocatalysis to refer to an enzyme as the biocatalyst, thereby neglecting the structural and metabolic framework provided by the cell. Here, the low whole-cell biocatalyst stability, that is, the stability of specific biocatalyst activity, in a process for the terminal oxyfunctionalization of renewable fatty acid methyl esters was investigated. This reaction, which is difficult to achieve by chemical means, is catalyzed by Escherichia coli featuring the monooxygenase system AlkBGT and the uptake facilitator AlkL from Pseudomonas putida GPo1...
November 7, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27878395/a-tale-of-two-methane-monooxygenases
#11
REVIEW
Matthew O Ross, Amy C Rosenzweig
Methane monooxygenase (MMO) enzymes activate O2 for oxidation of methane. Two distinct MMOs exist in nature, a soluble form that uses a diiron active site (sMMO) and a membrane-bound form with a catalytic copper center (pMMO). Understanding the reaction mechanisms of these enzymes is of fundamental importance to biologists and chemists, and is also relevant to the development of new biocatalysts. The sMMO catalytic cycle has been elucidated in detail, including O2 activation intermediates and the nature of the methane-oxidizing species...
November 22, 2016: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/27877090/genetic-variant-in-flavin-containing-monooxygenase-3-alters-lipid-metabolism-in-laying-hens-in-a-diet-specific-manner
#12
Jing Wang, Cheng Long, Haijun Zhang, Yanan Zhang, Hao Wang, Hongyuan Yue, Xiaocui Wang, Shugeng Wu, Guanghai Qi
Genetic variant T329S in flavin-containing monooxygenase 3 (FMO3) impairs trimethylamine (TMA) metabolism in birds. The TMA metabolism that under complex genetic and dietary regulation, closely linked to cardiovascular disease risk. We determined whether the genetic defects in TMA metabolism may change other metabolic traits in birds, determined whether the genetic effects depend on diets, and to identify genes or gene pathways that underlie the metabolic alteration induced by genetic and diet factors. We used hens genotyped as FMO3 c...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27873437/characterization-and-crystal-structure-of-a-robust-cyclohexanone-monooxygenase
#13
Elvira Romero, J Rubén Gómez Castellanos, Andrea Mattevi, Marco W Fraaije
Cyclohexanone monooxygenase (CHMO) is a promising biocatalyst for industrial reactions owing to its broad substrate spectrum and excellent regio-, chemo-, and enantioselectivity. However, the low stability of many Baeyer-Villiger monooxygenases is an obstacle for their exploitation in industry. Characterization and crystal structure determination of a robust CHMO from Thermocrispum municipale is reported. The enzyme efficiently converts a variety of aliphatic, aromatic, and cyclic ketones, as well as prochiral sulfides...
November 22, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27869336/use-of-the-synergist-piperonyl-butoxide-can-slow-the-development-of-alpha-cypermethrin-resistance-in-the-whitefly-bemisia-tabaci
#14
C T Zimmer, M Panini, K S Singh, E L Randall, L M Field, E Roditakis, E Mazzoni, C Bass
The development of insecticide resistance in insect pests of crops is a growing threat to sustainable food production, and strategies that slow the development of resistance are therefore urgently required. The insecticide synergist piperonyl butoxide (PBO) inhibits certain insect detoxification systems and so may delay the evolution of metabolic resistance. In the current study we characterized resistance development in the silverleaf whitefly, Bemisia tabaci, after selection with either a neonicotinoid (thiacloprid) or pyrethroid (alpha-cypermethrin) insecticide alone or in combination with PBO...
November 21, 2016: Insect Molecular Biology
https://www.readbyqxmd.com/read/27868112/uplc-ms-ms-investigation-of-%C3%AE-glucan-oligosaccharide-oxidation
#15
Samy Boulos, Laura Nyström
Polysaccharide degradation mediated by hydroxyl radicals (HO˙) or lytic monooxygenases (LPMOs) is relevant in various biological and industrial processes. Thereby, the Fenton-induced (H2O2/Fe(2+)) oxidation of mixed-linkage (1→3,1→4)-β-d-glucan (BG), a cereal dietary fibre with several well-established health promoting properties, shows potential for modulating BG functionality. The precise identification of oxidation products, however, is impeded by their diversity due to the indiscriminate nature of HO˙, the large molecular weight, and the corresponding low frequency of discrete alterations along the polymer chain...
November 21, 2016: Analyst
https://www.readbyqxmd.com/read/27863256/molecular-basis-of-thermostability-enhancement-of-renilla-luciferase-at-higher-temperatures-by-insertion-of-a-disulfide-bridge-into-the-structure
#16
Zahra Fanaei Kahrani, Rahman Emamzadeh, Mahboobeh Nazari, S Mohammad Mahdi Rasa
Renilla luciferase (RLuc), also known as Renilla-luciferin 2-monooxygenase, is a light producing enzyme used in many biotechnological applications such as bioreporters. However, its kinetics stability -especially at higher temperatures- is a limiting factor for developing thermostable bioreporters. The aim of this study was to improve the stability (at higher temperatures) of super Renilla luciferase 8 (SRLuc 8) which is a red-emitter variety of RLuc, by introduction of a disulfide bridge into its structure...
November 15, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27856636/gibepyrone-biosynthesis-in-the-rice-pathogen-fusarium-fujikuroi-is-facilitated-by-a-small-polyketide-synthase-gene-cluster
#17
Slavica Janevska, Birgit Arndt, Eva-Maria Niehaus, Immo Burkhardt, Sarah M Rösler, Nelson L Brock, Hans-Ulrich Humpf, Jeroen S Dickschat, Bettina Tudzynski
The 2H-pyran-2-one gibepyrone A and its oxidized derivatives gibepyrones B-F have been isolated from the rice pathogenic fungus Fusarium fujikuroi already more than 20 years ago. However, these products have not been linked to the respective biosynthetic genes and therefore, their biosynthesis has not been analyzed on a molecular level so far. Feeding experiments with isotopically labeled precursors clearly supported a polyketide origin for the formal monoterpenoid gibepyrone A, while the terpenoid pathway could be excluded...
November 17, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27851982/interdomain-electron-transfer-in-cellobiose-dehydrogenase-is-governed-by-surface-electrostatics
#18
Alan Kadek, Daniel Kavan, Julien Marcoux, Johann Stojko, Alfons K G Felice, Sarah Cianférani, Roland Ludwig, Petr Halada, Petr Man
BACKGROUND: Cellobiose dehydrogenase (CDH) is a fungal extracellular oxidoreductase which fuels lytic polysaccharide monooxygenase with electrons during cellulose degradation. Interdomain electron transfer between the flavin and cytochrome domain in CDH, preceding the electron flow to lytic polysaccharide monooxygenase, is known to be pH dependent, but the exact mechanism of this regulation has not been experimentally proven so far. METHODS: To investigate the structural aspects underlying the domain interaction in CDH, hydrogen/deuterium exchange (HDX-MS) with improved proteolytic setup (combination of nepenthesin-1 with rhizopuspepsin), native mass spectrometry with ion mobility and electrostatics calculations were used...
November 13, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27836848/investigation-of-the-role-played-by-cellobiose-dehydrogenases-from-podospora-anserina-during-lignocellulose-degradation
#19
Narumon Tangthirasunun, David Navarro, Sona Garajova, Didier Chevret, Laetitia Chan Ho Tong, Valérie Gautier, Kevin D Hyde, Philippe Silar, Jean-Guy Berrin
: Conversion of biomass into high-value products including biofuels is of great interest to develop sustainable biorefineries. Fungi are an inexhaustible source of enzymes to degrade plant biomass. Cellobiose dehydrogenases (CDHs) play an important role in the breakdown through synergistic action with fungal lytic polysaccharide monooxygenases (LPMOs). The three CDH genes of the model fungus Podospora anserina were inactivated resulting in singly and multiple CDH mutants. We detected almost no difference in the growth and fertility of the mutants on various lignocellulose sources, except on crystalline cellulose, where a two-fold decrease in fertility of the mutants lacking PaCDH1 and PaCDH2 was observed...
November 11, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27834801/trimethylamine-n-oxide-the-good-the-bad-and-the-unknown
#20
REVIEW
Manuel T Velasquez, Ali Ramezani, Alotaibi Manal, Dominic S Raj
Trimethylamine N-oxide (TMAO) is a small colorless amine oxide generated from choline, betaine, and carnitine by gut microbial metabolism. It accumulates in the tissue of marine animals in high concentrations and protects against the protein-destabilizing effects of urea. Plasma level of TMAO is determined by a number of factors including diet, gut microbial flora and liver flavin monooxygenase activity. In humans, a positive correlation between elevated plasma levels of TMAO and an increased risk for major adverse cardiovascular events and death is reported...
November 8, 2016: Toxins
keyword
keyword
52216
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"