Rendy Ruvindy, Penelope A Ajani, Sereena Ashlin, Gustaaf Hallegraeff, Kerstin Klemm, Christopher J Bolch, Sarah Ugalde, Mark Van Asten, Stephen Woodcock, Matthew Tesoriero, Shauna A Murray
Paralytic shellfish toxins (PSTs) produced by marine dinoflagellates significantly impact shellfish industries worldwide. Early detection on-farm and with minimal training would allow additional time for management decisions to minimize economic losses. Here, we describe and test a standardized workflow based on the detection of sxtA4 , an initial gene in the biosynthesis of PSTs. The workflow is simple and inexpensive and does not require a specialized laboratory. It consists of (1) water collection and filtration using a custom gravity sampler, (2) buffer selection for sample preservation and cell lysis for DNA, and (3) an assay based on a region of sxtA , DinoDtec lyophilized quantitative polymerase chain reaction (qPCR) assay...
April 12, 2024: Environmental Science & Technology