José Vicente Gil, Alberto Miralles, Sandra de Las Heras, Esperanza Such, Gayane Avetisyan, Álvaro Díaz-González, Marta Santiago, Carolina Fuentes, José María Fernández, Pilar Lloret, Irene Navarro, Pau Montesinos, Marta Llop, Eva Barragán
Introduction: Acute lymphoblastic leukemia (ALL) is a prevalent childhood cancer with high cure rate, but poses a significant medical challenge in adults and relapsed patients. Philadelphia-like acute lymphoblastic leukemia (Ph-like ALL) is a high-risk subtype, with approximately half of cases characterized by CRLF2 overexpression and frequent concomitant IKZF1 deletions. Methods: To address the need for efficient, rapid, and cost-effective detection of CRLF2 alterations, we developed a novel RT-qPCR technique combining SYBR Green and highresolution melting analysis on a single plate...
2024: Frontiers in Molecular Biosciences