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https://www.readbyqxmd.com/read/29219180/laminar-shear-stress-promotes-mitochondrial-homeostasis-in-endothelial-cells
#1
Li-Hong Wu, Hao-Chun Chang, Pei-Ching Ting, Danny Ling Wang
Vascular endothelial cells (ECs) are constantly subjected to flow-induced shear stress that is crucial for endothelial functions. Laminar shear stress (LSS) exerts atheroprotection to ECs. Mitochondrial homeostasis is essential for cellular survival. However, the effects of LSS on mitochondrial homeostasis in ECs remain unclear. Mitochondrial homeostasis in ECs exposed to LSS was examined. Cultured human umbilical vein ECs were subjected to LSS (12 dynes/cm2 ) generated by a parallel-plate flow chamber system...
December 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29199637/automated-3d-bioassembly-of-micro-tissues-for-biofabrication-of-hybrid-tissue-engineered-constructs
#2
Naveen Vijayan Mekhileri, Khoon Lim, Gabriella C J Brown, Isha Mutreja, Ben S Schon, Gary J Hooper, Tim B F Woodfield
Bottom-up biofabrication approaches combining micro-tissue fabrication techniques with extrusion-based 3D printing of thermoplastic polymer scaffolds are emerging strategies in tissue engineering. These biofabrication strategies support native self-assembly mechanisms observed in developmental stages of tissue or organoid growth as well as promoting cell-cell interactions and cell differentiation capacity. Few technologies have been developed to automate the precise assembly of micro-tissues or tissue modules into structural scaffolds...
December 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/29191246/munc18-1-gene-abnormalities-are-involved-in-neurodevelopmental-disorders-through-defective-cortical-architecture-during-brain-development
#3
Nanako Hamada, Ikuko Iwamoto, Hidenori Tabata, Koh-Ichi Nagata
While Munc18-1 interacts with Syntaxin1 and controls the formation of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) complex to regulate presynaptic vesicle fusion in developed neurons, this molecule is likely to be involved in brain development since its gene abnormalities cause early infantile epileptic encephalopathy with suppression-burst (Ohtahara syndrome), neonatal epileptic encephalopathy and other neurodevelopmental disorders. We thus analyzed physiological significance of Munc18-1 during cortical development...
November 30, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/29189218/adjacent-level-degeneration-bryan-total-disc-arthroplasty-versus-anterior-cervical-discectomy-and-fusion
#4
Justin Miller, Rick Sasso, Paul Anderson, K Daniel Riew, Austin McPhilamy, Thomas Gianaris
STUDY DESIGN: Prospective randomized, 2-center, clinical trial. OBJECTIVE: To prospectively compare adjacent level degeneration between the Bryan Cervical Disc Prosthesis and anterior cervical discectomy and fusion (ACDF), and assess the reliability of our measurements utilizing a method not previously described. SUMMARY OF BACKGROUND DATA: ACDF is a reliable and proven procedure for the treatment of radiculopathy and/or myelopathy. Despite a successful track record several limitations including adjacent segment degeneration/disease, loss of viscoelastic disc properties, perioperative immobilization, graft site morbidity, pseudarthrosis, and plating complications have been identified...
November 16, 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/29184051/clinical-efficacy-of-anterior-partial-corpectomy-and-titanium-mesh-fusion-and-internal-fixation-for-treatment-of-old-fracture-dislocation-of-the-lower-cervical-spine
#5
De-Chao Miao, Bao-Yang Zhang, Tao Lei, Yong Shen
BACKGROUND The aim of this study was to analyze the clinical features and to evaluate the efficacy of anterior partial corpectomy and titanium mesh fusion and internal fixation of old fracture dislocation of the lower cervical spine. MATERIAL AND METHODS We retrospectively analyzed the clinical data of 52 patients with old lower cervical fracture and dislocation treated with anterior partial corpectomy and titanium mesh fusion fixation between January 2008 and December 2013, with a mean follow-up period of 4...
November 29, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29182141/360%C3%A2-fixation-with-modern-instrumentations-of-segment-separation-cervical-spine-injury-in-a-23-month-old
#6
V Zs Kovari, J Vajda, R Veres
In this case report clinical and technical lessons including seven years follow up learned from a flexion-distraction, highly unstable cervical spine injury causing a complete separation of C6-7 cervical segment with tetraparesis in a 23-month-old boy, are presented. To our knowledge this is the only documented case in medical literature where adult size implants (cage, plate and lateral mass screw-rod system) were utilized for cervical combined anterior and posterior internal fixation in a child under the age of two years without implant-size related problems...
December 2016: Acta Orthopaedica Belgica
https://www.readbyqxmd.com/read/29180835/laminated-and-infused-parafilm%C3%A2-paper-for-paper-based-analytical-devices
#7
Yong Shin Kim, Yuanyuan Yang, Charles S Henry
Numerous fabrication methods have been reported for microfluidic paper-based analytical devices (μPADs) using barrier materials ranging from photoresist to wax. While these methods have been used with wide success, consistently producing small, high-resolution features using materials and methods that are compatible with solvents and surfactants remains a challenge. Two new methods are presented here for generating μPADs with well-defined, high-resolution structures compatible with solvents and surfactant-containing solutions by partially or fully fusing paper with Parafilm® followed by cutting with a CO2 laser cutter...
February 2018: Sensors and Actuators. B, Chemical
https://www.readbyqxmd.com/read/29174537/flow-cytometry-based-method-for-rapid-and-high-throughput-screening-of-hybridoma-cells-secreting-monoclonal-antibody
#8
Sachi Akagi, Chika Nakajima, Yoichiro Tanaka, Yasuyuki Kurihara
Monoclonal antibodies (mAbs) are a valuable biomaterial for basic life sciences and industrial purposes. The production of the mAb is time and effort intensive. In this report, we established a time- and labor-saving method for the mAb production. Because membrane-type immunoglobulin on a hybridoma cell surface and its secreted form, called as antibody, share the same binding property to the antigen, the fluorescence-labeled antigen bound to membrane-type immunoglobulin can be used as a screening marker. In the method, a hybridoma labeled by a fluorescent antigen was selected and sorted singly into 96-well plate using flow cytometer...
November 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29171978/comparison-between-zero-profile-spacer-and-plate-with-cage-in-the-treatment-of-single-level-cervical-spondylosis
#9
Tao Lan, Jian-Ze Lin, Shi-Yu Hu, Xin-Jian Yang, Yang Chen
BACKGROUND: Retrospective study of 68 patients of symptomatic cervical spondylosis who were treated by anterior cervical discectomy and fusion (ACDF). OBJECTIVE: The purpose of this study was to compare the clinical and radiological outcomes of patients with single level cervical spondylosis using either zero-profile spacer (group A) or anterior cervical plate and cage (group B). METHODS: Clinical and radiological data of 68 patients undergoing ACDF from C3-C7 were collected retrospectively...
November 17, 2017: Journal of Back and Musculoskeletal Rehabilitation
https://www.readbyqxmd.com/read/29163577/combination-of-the-endogenous-lhcsr1-promoter-and-codon-usage-optimization-boosts-protein-expression-in-the-moss-physcomitrella-patens
#10
Manuel Hiss, Lucas Schneider, Christopher Grosche, Melanie A Barth, Christina Neu, Aikaterini Symeonidi, Kristian K Ullrich, Pierre-François Perroud, Mareike Schallenberg-Rüdinger, Stefan A Rensing
The moss Physcomitrella patens is used both as an evo-devo model and biotechnological production system for metabolites and pharmaceuticals. Strong in vivo expression of genes of interest is important for production of recombinant proteins, e.g., selectable markers, fluorescent proteins, or enzymes. In this regard, the choice of the promoter sequence as well as codon usage optimization are two important inside factors to consider in order to obtain optimum protein accumulation level. To reliably quantify fluorescence, we transfected protoplasts with promoter:GFP fusion constructs and measured fluorescence intensity of living protoplasts in a plate reader system...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29163485/a-novel-affinity-tag-abtag-and-its-application-to-the-affinity-screening-of-single-domain-antibodies-selected-by-phage-display
#11
Greg Hussack, Toya Nath Baral, Jason Baardsnes, Henk van Faassen, Shalini Raphael, Kevin A Henry, Jianbing Zhang, C Roger MacKenzie
ABTAG is a camelid single-domain antibody (sdAb) that binds to bovine serum albumin (BSA) with low picomolar affinity. In surface plasmon resonance (SPR) analyses using BSA surfaces, bound ABTAG can be completely dissociated from the BSA surfaces at low pH, over multiple cycles, without any reduction in the capacity of the BSA surfaces to bind ABTAG. A moderate throughput, SPR-based, antibody screening assay exploiting the unique features of ABTAG is described. Anti-carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) sdAbs were isolated from a phage-displayed sdAb library derived from the heavy chain antibody repertoire of a llama immunized with CEACAM6...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29142629/a-prospective-study-with-cage-only-or-cage-with-plate-fixation-in-anterior-cervical-discectomy-and-interbody-fusion-of-one-and-two-levels
#12
Sam Yeol Kim, Seung Hwan Yoon, Dokeun Kim, Chang Hyun Oh, Seyang Oh
Objective: The authors prospectively analyzed the effect of one-level or two-level anterior cervical discectomy and fusion (ACDF), comparing stand-alone cages and cage-with-plate fixation constructs with respect to clinical outcomes and radiologic changes. Methods: A total of 84 patients who underwent one-level (n=52) or two-level ACDF (n=32) for cervical disc disease and who completed 2 years of follow-up were included in this study. The patients were divided by cervical level and grouped into ACDF-Cage-only and ACDF-Cage-with-plate groups...
November 2017: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/29142619/the-formation-of-extragraft-bone-bridging-after-anterior-cervical-discectomy-and-fusion-a-finite-element-analysis
#13
Shin Won Kwon, Chi Heon Kim, Chun Kee Chung, Tae Hyun Park, Su Heon Woo, Sung-Jae Lee, Seung Heon Yang
Objective: In addition to bone bridging inside a cage or graft (intragraft bone bridging, InGBB), extragraft bone bridging (ExGBB) is commonly observed after anterior cervical discectomy and fusion (ACDF) with a stand-alone cage. However, solid bony fusion without the formation of ExGBB might be a desirable condition. We hypothesized that an insufficient contact area for InGBB might be a causative factor for ExGBB. The objective was to determine the minimal area of InGBB by finite element analysis...
November 2017: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/29126037/radiographic-evaluation-of-first-mtp-joint-arthrodesis-for-severe-hallux-valgus-does-the-introduction-of-a-lag-screw-improve-union-rates-and-correction-of-the-intermetatarsal-angle
#14
Zachariah Pinter, Parke Hudson, Brent Cone, Girish Motwani, Krishna Prasad, Ashish Shah
BACKGROUND: First metatarsophalangeal (MTP-1) joint fusion is a reliable method for the correction of various deformities including hallux valgus and hallux rigidus. Ideal constructs provide high rates of fusion in desired alignment. The present study examines the union rates, as well as the change in dorsiflexion angle during the follow up period in patients who underwent MTP-1 fusion with a dorsal locking plate and a lag screw, versus patients fused with a dorsal locking plate alone...
December 2017: Foot
https://www.readbyqxmd.com/read/29120672/an-electrostatic-and-probabilistic-simulation-model-to-describe-neurosecretion-at-the-synaptic-scale
#15
A E Macias-Medri, Jacinto A Liendo, Ricardo J Silva
A hybrid simulation model (macro-molecular dynamics and Monte Carlo method) is proposed to reproduce neurosecretion and exocytosis. A theory has been developed for vesicular dynamics based on quasi-static electric interactions and a simple transition-state model for the vesicular fusion. Under the non-equilibrium electric conditions in an electrolytic fluid, it is considered that the motion of each synaptic vesicle is influenced by electrostatic forces exerted by the membranes of the synaptic bouton, other vesicles, the intracellular and intravesicular fluids, and external elements to the neuron...
November 9, 2017: Network: Computation in Neural Systems
https://www.readbyqxmd.com/read/29107411/fracture-of-fusion-mass-following-anterior-cervical-plate-removal-case-report
#16
Jonathan N Sellin, S Shelby Burks, Allan D Levi
We present the case of a delayed pseudoarthrosis resulting from a fracture at the site of a radiographically confirmed anterior cervical fusion following plate removal. In this case, an anterior cervical plate was removed to allow for further surgery at a supra-adjacent level. A modicum of literature exists describing delayed fractures following hardware removal in thoracolumbar fusion constructs. The development of a fracture/pseudoarthrosis following hardware removal at the site of a radiographically confirmed anterior cervical fusion has not been previously reported...
October 26, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/29093784/sagittal-alignment-of-a-strut-graft-affects-graft-subsidence-and-clinical-outcomes-of-anterior-cervical-corpectomy-and-fusion
#17
Koun Yamauchi, Kazunari Fushimi, Kei Miyamoto, Akira Hioki, Katsuji Shimizu, Haruhiko Akiyama
Study Design: Retrospective study. Purpose: The purpose of this study was to investigate the influence of sagittal alignment of the strut graft on graft subsidence and clinical outcomes after anterior cervical corpectomy and fusion (ACCF). Overview of Literature: ACCF is a common technique for the treatment of various cervical pathologies. Although graft subsidence sometimes occurs after ACCF, it is one cause for poor clinical results. Malalignment of the strut graft is probably one of the factors associated with graft subsidence...
October 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/29082779/role-of-extra-articular-tibiotalocalcaneal-arthrodesis-and-posterior-approach-in-highly-complex-cases
#18
Sergio Eckholt, Ruben Garcia-Elvira, Nicolás Fontecilla, Alvaro Fernandez-Reinales, Daniel Poggio
BACKGROUND: Tibiotalocalcaneal (TTC) arthrodesis is a procedure commonly used as salvage surgery for various pathologic processes that compromise the ankle and subtalar joints. It is a reasonably standardized procedure when performed as a primary surgery in advanced stages of TTC arthritis. For such cases, there are several alternative approaches, fixation materials, and bone substitutes that can be used. Most represent valid options with similar results in the literature. However, in highly complex cases requiring TTC arthrodesis, the options for the approach and fixation material can be limited...
October 1, 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/29079235/outcomes-of-locking-plate-fixation-for-hindfoot-fusion-procedures-in%C3%A2-15-patients
#19
Kenneth Smith, Ibukunoluwa Araoye, Caleb Jones, Ashish Shah
Tibiotalocalcaneal arthrodesis is a salvage procedure for various end-stage foot and ankle pathologic entities. Several factors are known to influence the union rate after these procedures, including construct rigidity. The data on locked plates as a fixation technique have been inconclusive, with variable union rates reported. One recent study suggested that locking plates can lead to high nonunion rates owing to excessive rigidity. The purpose of the present study was to retrospectively examine the outcomes of locking plate fixation...
November 2017: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29079230/first-metatarsophalangeal-joint-fusion-with-use-of-crossed-kirschner-wires-and-intramedullary-steinmann-pin
#20
Lawrence G Karlock, Levi Berry, Seth T Craft, Rocco Petrozzi, Adam G Grahn, Michael L Casteel
Primary arthrodesis is a thoroughly studied treatment option for end-stage pathologic entities of the first metatarsophalangeal joint. It is a commonly accepted treatment of many pathologic conditions, including hallux rigidus, severe hallux valgus, hallux varus, and other conditions pertaining to the first ray. Numerous fixation techniques are available for this procedure. Fixation constructs range from simple crossing Kirschner wires to plate and screw fixation or, even, external fixation. We propose a simple and cost-effective fixation technique using an intramedullary Steinmann pin with crossing Kirschner wires...
November 2017: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
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