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https://www.readbyqxmd.com/read/29677036/coupled-opg-fc-on-decellularized-aortic-valves-by-edc-nhs-attenuates-rat-mscs-calcification-in-vitro
#1
Qiao Zhang, Si Chen, Jiawei Shi, Fei Li, Xucong Shi, Xingjian Hu, Cheng Deng, Feng Shi, Guichun Han, Nianguo Dong
Valve calcification commonly damages natural human heart valves and tissue-engineered heart valves (TEHVs), and no ideal intervention is available in clinical practice. It is increasingly considered that osteoprotegerin (OPG) inhibits vascular calcification. We aimed to explore whether free OPG-Fc fusion protein or coupled OPG-Fc on decellularized aortic valves attenuates calcification. Calcification of rat bone marrow-derived mesenchymal stromal cells (MSCs) was induced by osteogenic differentiation media, and the effects of free OPG-Fc or OPG-Fc coupled on the decellularized porcine aortic heart valve leaflet scaffolds by coupling agents 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) on calcification were observed...
April 18, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29675505/locking-dorsal-plate-in-four-bone-arthrodesis-in-slac-and-snac-3-wrist
#2
Simona Odella, Amos M Querenghi, Francesco M Locatelli, Ugo Dacatra, Elia Creta, Pierluigi Tos
Purpose  The aim of this study was to evaluate the effectiveness and the safety of performing a four-bone arthrodesis (FBA) with dorsal locking plate in patients suffering from stage III scapholunate advanced collapse/scaphoid nonunion advance collapse (SLAC/SNAC) wrist. Methods  We evaluated retrospectively 20 patients surgically treated by a FBA with the use of locking dorsal plate. All the patients were clinically evaluated at follow-up for grip strength, range of motion, and pain (visual analog scale), and with the Disability of the Arm, Shoulder and Hand score and the Mayo wrist score...
March 2018: Joints
https://www.readbyqxmd.com/read/29671379/enhanced-degradation-of-perfluorooctanoic-acid-by-a-genome-shuffling-modified-pseudomonas-parafulva-yab-1
#3
Langbo Yi, Qingzhong Peng, Deming Liu, Lulu Zhou, Chongjian Tang, Yaoyu Zhou, Liyuan Chai
Perfluorooctanoic acid (PFOA) as an emerging persistent organic pollutant is hard to be degraded by conventional methods because of its stable physical and chemical properties. Microbial transformation is an attractive remediation approach to prevent and clean up PFOA contamination. To date, several strains of wild microbes have been reported to have limited capacity to degrade PFOA, Selection of superior strains degrading PFOA become urgently necessary. Here, we report the application of genome shuffling to improve the PFOA-degrading bacterium Pseudomonas Parafulva YAB-1...
April 19, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29665815/a-comparison-of-zero-profile-anchored-spacer-roi-c-and-plate-fixation-in-2-level-noncontiguous-anterior-cervical-discectomy-and-fusion-a-retrospective-study
#4
Zongyu Zhang, Yawei Li, Weimin Jiang
BACKGROUND: Anterior cervical discectomy and fusion (ACDF) is the classic surgical treatment for symptomatic cervical degenerative disc disease (CDDD). However, there is controversy over the best surgical management in patients with two noncontiguous symptomatic levels of CDDD. METHODS: From April 2011 to May 2014, 44 patients with two noncontiguous symptomatic levels of CDDD underwent skip-level ACDFs. In Group NoPlate, 23 cases underwent 2 noncontiguous levels of ACDF using zero-profile anchored spacer; and in Group Plate, 21 cases underwent 2 noncontiguous levels of ACDF using cages and plates...
April 17, 2018: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29656519/character-evolution-and-missing-morphological-data-across-asteridae
#5
Gregory W Stull, Melanie Schori, Douglas E Soltis, Pamela S Soltis
PREMISE OF THE STUDY: Our current understanding of flowering plant phylogeny provides an excellent framework for exploring various aspects of character evolution through comparative analyses. However, attempts to synthesize this phylogenetic framework with extensive morphological data sets have been surprisingly rare. Here, we explore character evolution in Asteridae (asterids), a major angiosperm clade, using an extensive morphological data set and a well-resolved phylogeny. METHODS: We scored 15 phenotypic characters (spanning chemistry, vegetative anatomy, and floral, fruit, and seed features) across 248 species for ancestral state reconstruction using a phylogenetic framework based on 73 plastid genes and the same 248 species...
April 14, 2018: American Journal of Botany
https://www.readbyqxmd.com/read/29655650/surgical-treatment-of-lisfranc-injury-with-plantar-plate-approach
#6
Deepal Dalal, Christian Curry, Ryan Carter, Colin Zdenek, Nicholas W Todd, Shannon M Rush, Richard Jensen
Midfoot injuries are the second most common athletic foot injury documented in the published data. High-energy Lisfranc dislocations are commonly seen secondary to traumatic etiologies and disrupt the strong midfoot ligaments supporting the arch. These injuries require immediate surgical intervention to prevent serious complications such as compartment syndrome and amputation. The present case series reports a new Lapidus plate system used in 3 patients who underwent arthrodesis procedures for Lisfranc joint dislocation...
April 11, 2018: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29619844/comparison-of-screws-to-plate-and-screw-constructs-for-midfoot-arthrodesis
#7
Jamal Ahmad, Mary-Katherine Lynch, Mitchell Maltenfort
BACKGROUND: We performed a prospective comparison of screws versus plate-and-screws for midfoot arthrodesis. MATERIALS: Between 2010 and 2015, a total of 50 patients with midfoot arthritis received screws or plate-and-screws for their midfoot arthrodesis. Function and pain were graded with the Foot and Ankle Ability Measures (FAAM) and visual analog scale (VAS), respectively. Data regarding arthrodesis healing and complications were recorded. RESULTS: Twenty-five patients received screws for fusion, where 21 achieved full arthrodesis healing by 6 months from surgery...
April 1, 2018: Foot & Ankle International
https://www.readbyqxmd.com/read/29613929/long-term-outcomes-of-glenohumeral-arthrodesis
#8
Eric R Wagner, Richard McLaughlin, Shumaila Sarfani, Robert H Cofield, John W Sperling, Joaquin Sanchez-Sotelo, Bassem T Elhassan
BACKGROUND: Glenohumeral arthrodesis is associated with a high rate of complications. Although patients experience reasonable pain relief and shoulder stability, they experience marked limitations in their upper-extremity function. The purpose of this study was to examine the clinical outcomes of glenohumeral arthrodesis. METHODS: Twenty-nine patients with 29 affected shoulders underwent primary glenohumeral arthrodesis between 1992 and 2009. Surgical indications included rotator cuff arthropathy and pseudoparalysis (n = 7), neurologic injuries (n = 12), chronic infection (n = 3), recurrent dislocations (n = 3), and proximal humeral or shoulder girdle tumors (n = 4)...
April 4, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29609081/biomechanical-analysis-of-lateral-lumbar-interbody-fusion-constructs-with-various-fixation-options-based-on-a-validated-finite-element-model
#9
Zhenjun Zhang, Guy R Fogel, Zhenhua Liao, Yitao Sun, Weiqiang Liu
BACKGROUND: Lateral lumbar interbody fusion using cage supplemented with fixation has been widely used in the treatment of lumbar disease. A combined fixation of lateral plate and spinous process plate may provide multiplanar stability similar to that of bilateral pedicle screws, and may reduce morbidity. The biomechanical influence of the combined fixation on cage subsidence and facet joint stress has not been well described. The aim of this study was to compare biomechanics of various fixation options and to verify biomechanical effects of the combined fixation...
March 30, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29604552/biologically-synthesized-titanium-oxide-nanostructures-combined-with-morphogenetic-protein-as-wound-healing-agent-in-the-femoral-fracture-after-surgery
#10
Yushu Zhang, Chuanlian Zhang, Kemiao Liu, Xia Zhu, Fang Liu, Xiaofen Ge
The aim of the present study is to develop novel approach for the green synthesis of titanium oxide nanoparticles (TiO2 NPs) using Eichhornia crassipes extract and calcined at different temperatures for evaluate the wound healing activity in the femoral fracture. The synthesized TiO2 are formed different (plate and rod-like) nanostructures at various calcination temperatures. These samples were characterized by X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FTIR), Field emission scanning electron microscope (FE-SEM) and transmission electron microscope (TEM)...
March 6, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29588241/facet-joint-fixation-and-alif-dlif-or-tlif-for-treatment-of-degenerative-lumbar-disc-diseases-retrospective-cohort-study-of-a-new-minimally-invasive-technique
#11
Evgenii Belykh, Andrey A Kalinin, Nikolay L Martirosyan, Talgat Kerimbayev, Nicholas Theodore, Mark C Preul, Vadim A Byvaltsev
BACKGROUND: Anterior, direct lateral and transforaminal lumbar interbody fusions (ALIF, DLIF, and TLIF) are usually combined with posterior fixation to treat degenerative spinal diseases. Outcomes of ALIF, TLIF, or DLIF combined with a new wedge-shaped, interfacet cage-plate have not been reported. We assessed early clinical outcomes of patients treated with interbody fusion and facet fixation using a titanium wedge-shaped cage-plate. METHODS: This retrospective observational cohort study included patients (n=80) who underwent 1-level interbody fusion and facet joint fixation via ALIF (n=24) or DLIF (n=26) with bilateral facet fixation or TLIF with ipsilateral pedicle screws and contralateral facet fixation (n=30)...
March 24, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29576918/nonspanning-total-wrist-arthrodesis-with-a-low-profile-locking-plate
#12
Schneider K Rancy, Eugene T Ek, Sophia Paul, Robert N Hotchkiss, Scott W Wolfe
Purpose  This study aims to compare the outcomes and complications of our technique for nonspanning total wrist arthrodesis using a locking plate with the standard carpometacarpal spanning technique. Methods  A retrospective review of charts was performed to identify patients who underwent total wrist arthrodesis by the senior author (S.W.W.). We compared the outcomes of 15 cases of nonspanning wrist fusion with a 2.4/2.7 mm locking T plate to 11 cases of spanning wrist fusion with a 2.7/3.5 mm locking compression plate...
April 2018: Journal of Wrist Surgery
https://www.readbyqxmd.com/read/29572179/continuous-application-of-compressive-force-induces-fusion-of-osteoclast-like-raw264-7-cells-via-upregulation-of-rank-and-downregulation-of-lgr4
#13
Rieko Matsuike, Hideki Tanaka, Kumiko Nakai, Mai Kanda, Maki Nagasaki, Fumiko Murakami, Chika Shibata, Kotoe Mayahara, Akira Nakajima, Natsuko Tanabe, Takayuki Kawato, Masao Maeno, Noriyoshi Shimizu
AIMS: During orthodontic treatment, facilitating osteoclastic bone resorption in the alveolar bone exposed to the compressive force (CF) is an important factor for tooth movement. The present study investigated the effect of CF stimulation on the differentiation of RAW264.7 cells from precursors to mature osteoclasts. MAIN METHODS: The cells were continuously stimulated with 0.3, 0.6, or 1.1 g/cm2 CF-which was generated by increasing the volume of culture medium in the wells of a 96-well plate-in the presence or absence of receptor activator of nuclear factor κB (RANK) ligand (RANKL) for 4 days...
March 20, 2018: Life Sciences
https://www.readbyqxmd.com/read/29562329/regulation-of-expression-of-a-select-group-of-bacillus-anthracis-spore-coat-proteins
#14
Arthur Aronson
The spore coat of Bacilli is a relatively complex structure comprised of about 70 species of proteins in 2 or 3 layers. While some are involved in assembly or protection, the regulation of many are not well defined so lacZ transcriptional fusions were constructed to six Bacillus anthracis spore coat genes in order to gain insight into their possible functions. The genes were selected on the basis of the location of the encoded proteins within the coat and distribution among spore forming species. Conditions tested were temperature and media either as solid or liquid...
March 17, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29558996/management-of-irreducible-unilateral-facet-joint-dislocations-in-subaxial-cervical-spine-two-case-reports-and-a-review-of-the-literature
#15
Yu Zhou, Zhenyu Zhou, Lifeng Liu, Xuecheng Cao
BACKGROUND: Skeletal and soft tissue damage are often associated with unilateral facet dislocations, which undoubtedly lead to instability of the spine and further increase difficulties in cervical reduction. This type of irreducible facet dislocation is usually accompanied with potential catastrophic consequences including neurological deficit and severe disability. Therefore, a consistent and evidence-based treatment plan is imperative. CASE PRESENTATION: The literature regarding the management of traumatic unilateral locked cervical facet dislocations was reviewed...
March 21, 2018: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/29557926/stiffness-matters-part-ii-the-effects-of-plate-stiffness-on-load-sharing-and-the-progression-of-fusion-following-acdf-in-vivo
#16
Joshua M Peterson, Carolyn Chlebek, Ashley M Clough, Alexandra K Wells, Kathleen E Batzinger, John M Houston, Katerina Kradinova, Joseph C Glennon, Darryl J DiRisio, Eric H Ledet
STUDY DESIGN: Real time in vivo measurement of forces in the cervical spine of goats following anterior cervical discectomy and fusion (ACDF). OBJECTIVE: To measure interbody forces in the cervical spine during the time course of fusion following ACDF with plates of different stiffnesses. SUMMARY OF BACKGROUND DATA: Following ACDF, the biomechanics of the arthrodesis is largely dictated by the plate. The properties of the plate prescribe the extent of load-sharing through the disc space versus the extent of stress-shielding...
March 19, 2018: Spine
https://www.readbyqxmd.com/read/29547464/stiffness-matters-part-i-the-effects-of-plate-stiffness-on-the-biomechanics-of-acdf-in-vitro
#17
Joshua M Peterson, Carolyn Chlebek, Ashley M Clough, Alexandra K Wells, Eric H Ledet
STUDY DESIGN: In vitro biomechanical testing of human cadaveric cervical and goat cervical motion segments. OBJECTIVE: To measure the effects of plate stiffness on load-sharing, instantaneous axis of rotation (IAR), and posterior element loading after anterior cervical discectomy and fusion (ACDF). SUMMARY OF BACKGROUND DATA: ACDF is intended to create an environment which facilitates sufficient stability and biomechanical conditions to promote bone formation...
March 15, 2018: Spine
https://www.readbyqxmd.com/read/29534563/antibiotic-combinations-that-enable-one-step-targeted-mutagenesis-of-chromosomal-genes
#18
Wonsik Lee, Truc Do, Ge Zhang, Daniel Kahne, Timothy C Meredith, Suzanne Walker
Targeted modification of bacterial chromosomes is necessary to understand new drug targets, investigate virulence factors, elucidate cell physiology, and validate results of -omics-based approaches. For some bacteria, reverse genetics remains a major bottleneck to progress in research. Here we describe a compound-centric strategy that combines new negative selection markers with known positive selection markers to achieve simple, efficient one-step genome engineering of bacterial chromosomes. The method was inspired by the observation that certain non-essential metabolic pathways contain essential late steps, suggesting that antibiotics targeting a late step can be used to select for the absence of genes that control flux into the pathway...
March 13, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29533035/-comparison-of-clinical-effects-between-anterior-cervical-discectomy-combined-with-corpectomy-and-cervical-posterior-single-open-door-laminoplasty-in-treating-three-segment-cervical-spondylotic-myelopathy
#19
Jia-Fu Zhu, Ning Yan, Wei-Xing Xu, Tie-Sheng Hou, Shun-Zhi Yu, Hong Liu, Li-Feng Zhai
OBJECTIVE: To compare the clinical effects between anterior cervical discectomy and fusion(ACDF) combined with anterior cervical corpectomy and fusion(ACCF) and cervical posterior single open-door laminoplasty with mini-titanium plate fixation in treating three-segment cervical spondylotic myelopathy. METHODS: The clinical data of 63 patients (39 males and 24 females) with three-segment cervical spondylotic myelopathy underwent surgical treatment from March 2014 to March 2016 were retrospectively analyzed...
January 25, 2018: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29526639/the-effect-of-inter-body-fusion-cage-design-on-the-stability-of-the-instrumented-spine-in-response-to-cyclic-loading-an-experimental-study
#20
Ron N Alkalay, Robert Adamson, Michael W Groff
BACKGROUND CONTEXT: In the lumbar spine, end plate preparation for the interbody fusion cages may critically affect the cage's long term performance. This study investigated the effect of the interbody cage design on the compliance and cage subsidence of instrumented spines under cyclic compression. PURPOSE: To quantify the role of cage geometry and bone density on the stability of the spinal construct in response to cyclic compressive loads. STUDY DESIGN: Changes in the cage-bone interface and the effect of bone density on these changes were evaluated in a human cadaveric model for three intervertebral cage designs...
March 8, 2018: Spine Journal: Official Journal of the North American Spine Society
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