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https://www.readbyqxmd.com/read/29452282/load-sharing-through-elastic-micro-motion-accelerates-bone-formation-and-interbody-fusion
#1
Eric H Ledet, Glenn P Sanders, Darryl J DiRisio, Joseph C Glennon
BACKGROUND: Context: Achieving a successful spinal fusion requires the proper biologic and biomechanical environment. Optimizing load-sharing in the interbody space can enhance bone formation. For anterior cervical discectomy and fusion (ACDF), loading and motion are largely dictated by the stiffness of the plate which can facilitate a balance between stability and load-sharing. The advantages of load-sharing may be substantial for patients with comorbidities and in multi-level procedures where pseudarthrosis rates are significant...
February 13, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29443092/single-cell-quantification-of-protein-degradation-rates-by-time-lapse-fluorescence-microscopy-in-adherent-cell-culture
#2
Andrea Brigitta Alber, David Michael Suter
Proteins are in a dynamic state of synthesis and degradation and their half-lives can be adjusted under various circumstances. However, most commonly used approaches to determine protein half-life are either limited to population averages from lysed cells or require the use of protein synthesis inhibitors. This protocol describes a method to measure protein half-lives in single living adherent cells, using SNAP-tag fusion proteins in combination with fluorescence time-lapse microscopy. Any protein of interest fused to a SNAP-tag can be covalently bound by a fluorescent, cell permeable dye that is coupled to a benzylguanine derivative, and the decay of the labeled protein population can be monitored after washout of the residual dye...
February 4, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29432408/a-randomized-trial-comparing-clinical-outcomes-between-zero-profile-and-traditional-multilevel-anterior-cervical-discectomy-and-fusion-surgery-for-cervical-myelopathy
#3
Shenghua He, Hualong Feng, Zhiming Lan, Juyi Lai, Zhitao Sun, Yeguang Wang, Jian Wang, Zhiqiang Ren, Feiqiang Huang, Fuguang Xu
STUDY DESIGN: Prospective randomized study from a single center OBJECTIVE.: The purpose of this study was to compare outcomes between a zero-profile (ZP) anterior cervical discectomy and fusion (ACDF) construct to a traditional ACDF with anterior cervical plate (ACP) in the treatment of multilevel cervical spondylotic myelopathy. SUMMARY OF BACKGROUND DATA: Multilevel cervical spondylotic myelopathy can be treated by a variety of techniques, most commonly with corpectomy, ACDF, posterior cervical fusion, or a combination of the three...
March 1, 2018: Spine
https://www.readbyqxmd.com/read/29417771/-comparison-of-clinical-effects-between-anterior-cervical-zero-incision-fusion-system-and-traditional-nail-plate-system-in-the-treatment-of-cervical-spondylotic-myelopathy
#4
Bu-Qing Chang, Hu Feng, Chao-Jiang Yu, Kai Huang, Xiao Gao, Hao Tang, Yun-Chang Jiang
OBJECTIVE: To compare the short-term efficacy of anterior cervical discectomy and fusion(ACDF) with traditional nail plate system and Zero-profile device in the treatment of cervical spondylotic myelopathy(CSM). METHODS: The clinical data of 45 patients with CSM treated from July 2014 to August 2015 was retrospectively analyzed. There were 23 males and 22 females with an average age of 53.7 years old(range, 32 to 71 years old). The course of disease was 5 months to 2 years...
May 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29417047/a-computed-microtomography-method-for-understanding-epiphyseal-growth-plate-fusion
#5
Katherine A Staines, Kamel Madi, Behzad Javaheri, Peter D Lee, Andrew A Pitsillides
The epiphyseal growth plate is a developmental region responsible for linear bone growth, in which chondrocytes undertake a tightly regulated series of biological processes. Concomitant with the cessation of growth and sexual maturation, the human growth plate undergoes progressive narrowing, and ultimately disappears. Despite the crucial role of this growth plate fusion "bridging" event, the precise mechanisms by which it is governed are complex and yet to be established. Progress is hindered by the current methods for growth plate visualization; these are invasive and largely rely on histological procedures...
January 23, 2018: Frontiers in Materials
https://www.readbyqxmd.com/read/29414245/bifunctional-fusion-proteins-containing-the-sequence-of-the-bradykinin-homologue-maximakinin-activities-at-the-rat-bradykinin-b-sub-2-sub-receptor
#6
Xavier Charest-Morin, Robert Lodge, François Marceau
To support bradykinin (BK) B2 receptor (B2R) detection and therapeutic stimulation, we developed and characterized fusion proteins consisting of the BK homolog maximakinin (MK), or variants, positioned at the C-terminus of functional proteins (enhanced green fluorescent protein (EGFP), the peroxidase APEX2 or human serum albumin (HSA)). EGFP-MK loses its reactivity with anti-BK antibodies and molecular mass as it progresses in the endosomal tract of cells expressing rat B2Rs (immunoblots, epifluorescence microscopy)...
February 7, 2018: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/29409296/first-metatarsophalangeal-fusion-using-joint-specific-dorsal-plate-with-interfragmentary-screw-augmentation-clinical-and-radiological-outcomes
#7
Ahmed Latif, Baljinder S Dhinsa, Benjamin Lau, Ali Abbasian
BACKGROUND: This study reports the outcome of a plating system for arthrodesis of the first metatarsophalangeal joint (1st MTPJ) that incorporates a lag compression screw within a low profile titanium plate with a predetermined contour. This is the first report of the outcomes of this implant from a non-affiliated centre. PATIENT AND METHODS: This is a prospective cohort study of 40 consecutive primary 1st MTPJ arthrodesis procedures. The mean age of the cohort was 56 years (range, 20-74 years)...
October 14, 2017: Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29409218/use-of-masquelet-s-technique-for-treating-the-first-metatarsophalangeal-joint-in-cases-of-gout-combined-with-a-massive-bone-defect
#8
Feng Liu, Ruo-Kun Huang, Ming Xie, Hao Pan, Jing-Jing Zhao, Bo Lei
BACKGROUND: To examine the safety and efficacy of Masquelet's technique as a surgical method for treating the first metatarsophalangeal joint in cases of gout accompanied by a massive bone defect. METHODS: From January 2010 to January 2016, eleven patients (7 males and 4 females; mean age 33.1 years; range, 23-43 years) received surgical treatment for a first metatarsophalangeal joint tophus which caused a serious bone defect. The first metatarsophalangeal bone defects ranged from 3-6cm, or nearly 50% of the length of normal bone...
February 17, 2017: Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29409198/result-of-iofix-intra-osseous-fixation-device-for-first-metatarsophalangeal-joint-arthrodesis-a-single-surgeon-s-series
#9
Rohit Singhal, Tariq Kwaees, Mohamed Mohamed, Miltiadis Argyropoulos, Pradeep Amarasinghe, E M Toh
BACKGROUND: Arthrodesis of the first metatarsophalangeal joint (MTPJ) is a common procedure to treat significant first MTPJ arthritis. However, dorsal plates used for this have been associated with high incidence of metalwork removal. The IOFIX (Intra-Osseous FIXation) is a fixed angle device that is noted to provide a more uniform compression over a larger aspect of the fusion surfaces than a screw construct alone with the advantage of minimizing soft tissue irritation which can reduce the need for subsequent implant removal...
June 1, 2017: Foot and Ankle Surgery: Official Journal of the European Society of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/29407072/poor-outcomes-of-fusion-with-trabecular-metal-implants-after-failed-total-ankle-replacement-early-results-in-11-patients
#10
S Aubret, L Merlini, M Fessy, J L Besse
INTRODUCTION: One of the reasons for revision of total ankle replacement (TAR) implants is loosening due to subchondral cysts. Reconstruction and fusion of the ankle is often the first choice for revision procedures due to the large bone defects, which are typically filled with autograft and/or allograft. Filling the defect with a trabecular metal tantalum implant is a potential alternative given the biomechanical properties of this component. HYPOTHESIS: Using tantalum as a spacer provides primary stability and contributes to fusion of the ankle joint after removal of failed TAR implants...
January 30, 2018: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/29403251/transoral-screw-and-wire-fixation-for-unstable-anterior-%C3%A2-atlas-fracture
#11
Semih Keskil, Murat Göksel, Ulas Yüksel
Study Design: Atlas fractures are evaluated according to the fracture type and ligamentous injury. External immobilization may result in fracture nonunion. Objective: The ideal treatment method for non-stabilized atlas fractures is limited fixation without restricting the range of motion of the atlantoaxial and atlantooccipital joints. Summary of Background Data: Such a result can be established by using either anterior fixation or posterior lateral mass fixation...
October 2017: Journal of Craniovertebral Junction and Spine
https://www.readbyqxmd.com/read/29403246/surgical-management-of-c-type-subaxial-cervical-fractures-using-cervical-traction-followed-by-anterior-cervical-discectomy-and-fusion-within-12-h-after-the-trauma
#12
Pasquale Donnarumma, Vincenzo Bozzini, Gaetano Rizzi, Arturo Berardi, Gaetano Merlicco
Study Design: This was a retrospective cohort study. Objective: To report our 10-year experience of closed reduction using Crutchfield traction followed by anterior cervical discectomy and fusion within 12 h from injury for C-type subaxial cervical fractures (according to the AOSpine classification system). Methods: Clinical records and neuroimaging were retrospectively reviewed. Surgical details were provided. Results: A total of 22 patients were included in the study...
October 2017: Journal of Craniovertebral Junction and Spine
https://www.readbyqxmd.com/read/29397412/resection-arthrodesis-using-distraction-osteogenesis-then-plating-as-a-hybrid-surgical-technique-for-the-management-of-bone-sarcomas-of-the-distal-tibia
#13
Teng-Fei Lou, Hua Li, Yi-Min Chai, Chun-Yang Wang, Sheng-He Liu, Musha Hamushan, Fan Wu, Wei-Jie Cai, Pei Han
PURPOSE: We report the oncological and functional results of limb salvage for bone sarcomas involving the distal tibia using hybrid surgical technique of resection arthrodesis by bone transport then plating. METHODS: Five patients (mean age 18.6 years) with primary distal tibial sarcomas (two Ewing's sarcomas and three osteosarcomas) were treated by this method. The average duration of follow-up is 53 months. All patients accepted distraction osteogenesis with a standard technique using external fixator after wide (four cases) or marginal (one case) resection in the first operation...
February 3, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29394936/risk-factors-for-non-fusion-segment-disease-after-anterior-cervical-spondylosis-surgery-a-retrospective-study-with-long-term-follow-up-of-171-patients
#14
Ziqiang Wang, Liangliang Zhou, Bin Lin, Keran Song, Qinghe Niu, Dongfeng Ren, Jiaguang Tang
BACKGROUND: The purpose of this study was to investigate the incidence and causes of non-fusion segment disease (NFSD), both adjacent and non-adjacent to a fused segment, after anterior cervical arthrodesis. METHODS: This is a single-center study. Between January 1998 and January 2011, two surgeons' 171 patients who had an anterior cervical decompression and fusion were followed clinically for more than 5 years. The correlation between the incidence of symptomatic non-fusion segment disease and the following clinical parameters (age at operation, fusion levels,) and radiological parameters (number of patients who had a plate, anterior cervical decompression and fusion (ACDF) or corpectomies, preoperative and postoperative cervical spine alignment, Pavlov's ratio at the C5 level, and preoperative existence of a non-fusion segment degeneration on magnetic resonance imaging) was evaluated...
February 2, 2018: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29384883/comparison-of-the-clinical-effects-of-zero-profile-anchored-spacer-roi-c-and-conventional-cage-plate-construct-for-the-treatment-of-noncontiguous-bilevel-of-cervical-degenerative-disc-disease-cddd-a-minimum-2-year-follow-up
#15
Yingjie Lu, Weiguo Bao, Zongyi Wang, Feng Zhou, Jun Zou, Weimin Jiang, Huilin Yang, Zhiming Zhang, Xuesong Zhu
Comparing the clinical and radiographic outcomes in anterior cervical discectomy and fusion (ACDF) using a zero-profile anchored spacer (ROI-C) or a conventional cage-plate construct (CPC) for treating noncontiguous bilevel of cervical degenerative disc disease (CDDD).Overall, 46 patients with 2 noncontiguous segments of CDDD, treated with ACDF from January 2011 to October 2015, were included in this study. ROI-C was used in 22 patients (group A) and CPC in 24 patients (group B). The clinical and radiographic outcomes and complications were compared pre- and postoperatively...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29384855/anterior-corpectomy-and-reconstruction-using-dynamic-cervical-plate-and-titanium-mesh-cage-for-cervical-spondylotic-myelopathy-a-minimum-5-year-follow-up-study
#16
Junfeng Zeng, Yuchen Duan, Yi Yang, Beiyu Wang, Ying Hong, Jigang Lou, Ning Ning, Hao Liu
Anterior cervical corpectomy and fusion (ACCF) is an effective surgical technique for cervical spondylotic myelopathy (CSM). However, no data exist regarding long-term outcomes after ACCF with the dynamic cervical plate for CSM. This study aimed to provide minimum 5-year clinical and radiographic outcomes of anterior corpectomy and reconstruction using dynamic cervical plate and titanium mesh cage (TMC) for CSM.Thirty-five patients who underwent single- or 2-level ACCF with dynamic cervical plate and TMC for the treatment of CSM were retrospectively investigated...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29382028/intraoperative-conversion-of-artificial-cervical-disc-replacement-to-anterior-cervical-discectomy-and-fusion-for-iatrogenic-fracture-a-rare-case-report
#17
Bei-Yu Wang, Ting-Kui Wu, Hao Liu, Yu-Xiao Deng, Chen Ding
RATIONALE: Artificial cervical disc replacement (ACDR) has been reported to be an effective and safe surgical treatment for cervical spondylosis. However, iatrogenic fracture during ACDR surgery has rarely been reported. Considering its infrequency, we report a rare clinical case, with the aim of sharing our experience and exploring when to convert ACDR to fusion intraoperatively. PATIENTS CONCERNS: A 47-year-old woman felt neck pain with weakness and numbness in both upper limbs for 5 months...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29368613/comparison-among-perfect-c%C3%A2-zero-p%C3%A2-and-plates-with-a-cage-in-single-level-cervical-degenerative-disc-disease
#18
Sung Hyun Noh, Ho Yeol Zhang
BACKGROUND: We intended to analyze the efficacy of a new integrated cage and plate device called Perfect-C for anterior cervical discectomy and fusion (ACDF) to cure single-level cervical degenerative disc disease. METHODS: We enrolled 148 patients who were subjected to single-level ACDF with one of the following three surgical devices: a Perfect-C implant (41 patients), a Zero-P implant (36 patients), or a titanium plate with a polyetheretherketone (PEEK) cage (71 patients)...
January 25, 2018: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29366638/3-4-diaminopyridine-reverses-paralysis-in-botulinum-neurotoxin-intoxicated-diaphragms-through-two-functionally-distinct-mechanisms
#19
Aaron B Bradford, James B Machamer, Trisha M Russo, Patrick M McNutt
Botulinum neurotoxins (BoNTs) are exceedingly potent neurological poisons that prevent neurotransmitter release from peripheral nerve terminals by cleaving presynaptic proteins required for synaptic vesicle fusion. The ensuing neuromuscular paralysis causes death by asphyxiation. Although no antidotal treatments exist to block toxin activity within the nerve terminal, aminopyridine antagonists of voltage-gated potassium channels have been proposed as symptomatic treatments for botulism toxemia. However, clinical evaluation of aminopyridines as symptomatic treatments for botulism has been inconclusive, in part because mechanisms responsible for reversal of paralysis in BoNT-poisoned nerve terminals are not understood...
January 20, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29354737/a-radiographic-follow-up-study-of-stand-alone-cage-and-graft-plate-constructs-for-single-level-anterior-cervical-discectomy-and-fusion
#20
Joseph F Baker, Jaime Gomez, Kartik Shenoy, Sarah Kim, Afshin Razi, Yong Kim
Background: Anterior cervical discectomy and fusion (ACDF) may be performed using an interbody cage or graft with an anterior plate or with a stand-alone (SA) interbody device without the anterior plate. The pros and cons of each vary. This study examined the radiographic outcome of the two techniques with a focus on implant subsidence. Methods: A retrospective review of cases of singe level ACDF by a single surgeon was undertaken. Medical and radiographic records were reviewed to determine subsidence, pre- and post-operative segmental and total lordosis in cohorts of both stand-alone and graft-and-plate constructs...
December 2017: Journal of Spine Surgery (Hong Kong)
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